Rmu_sc0006656.1_g000001

squalene

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006656.1
OG0000386
Physical Location & Seq
Forward (+)
1 .. 2603
2603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006656.1_g000001.1.cds

Sequence Viewer

Length: 767 bp
acaaggtgttttgtgcatcacctgatccggcaagacaggaaatgcgtgaagcctgtttcggctatttgagccttggaggcatctgctcctatggaccagtatctcttctctctggtcttaaccctcgtccattacacttgtttctccatttctttgctgttgccatctatggtgtcggccgcttactgtttccattcccttcacctaaacgcatgtggcttgggattcgattgatcttgtcaaaagccaaaatggctttggcttttccggaagccatctccaacaactcactaagtttagccattttgacttctggagtcattttgacgaagggagctggaacgaaagccatattggctgaggaaggaatcggctgggccccagttgcacatgggttttctttggaacacagacacaacgcgcagagggagtctgaatccctcaaacgcgcaaaaggacaaaataacgtcttggagggggagaaaagcgccatacacgcgcaagactttggccgacactgggccccgccagtgatagaagtcgttggggcttccaacgacggctctaagggtactagtcaagagaaaaaaaaattgtgggagcgtaagtatacgagacttgcaagcctgccggaggtgtggttagattgggagaagggtgtgacggtcgttggaaaaagattagaccggcatgccaaagatggcgtcaagctcttaacaaagcggcacttcttcgaggaagtggtgataacgccaccgacgaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko00909 ko01100 ko01110 ko01130 map00100 map00909 map01100 map01110 map01130
Pfam Domains
Protein Families
SE

Protein Analysis

254

Amino Acids

28.04

Weight (kDa)

9.76

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000386)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g44301 FvH4_5g19180 FvH4_6g21610 FvH4_6g22213 FvH4_6g33100 FvH4_6g34882
malus_domestica MD00G1014200.v1.1 MD04G1097600.v1.1 MD13G1034300.v1.1
pyrus_communis pycom01g05740 pycom04g10190 pycom05g05910 pycom05g14840 pycom07g10040 pycom08g16560 pycom09g00370 pycom10g03940 pycom10g15460 pycom11g15220 pycom12g07590 pycom14g10750 pycom14g14610 pycom15g20370
rosa_chinensis RchiOBHm_Chr4g0392971 RchiOBHm_Chr4g0409971 RchiOBHm_Chr5g0059411 RchiOBHm_Chr6g0305091 RchiOBHm_Chr7g0202551 RchiOBHm_Chr7g0242211
rosa_laevigata RLG00000003630 RLG00000016594
rosa_multiflora Rmu_sc0000018.1_g000003 Rmu_sc0000288.1_g000031 Rmu_sc0000327.1_g000003 Rmu_sc0000361.1_g000009 Rmu_sc0000546.1_g000077 Rmu_sc0000616.1_g000024 Rmu_sc0000623.1_g000005 Rmu_sc0000704.1_g000009 Rmu_sc0000749.1_g000026 Rmu_sc0000965.1_g000012 Rmu_sc0001144.1_g000024 Rmu_sc0001144.1_g000026 Rmu_sc0001159.1_g000053 Rmu_sc0001348.1_g000017 Rmu_sc0001585.1_g000015 Rmu_sc0001651.1_g000017 Rmu_sc0001832.1_g000013 Rmu_sc0001838.1_g000003 Rmu_sc0001977.1_g000020 Rmu_sc0002983.1_g000011 Rmu_sc0003270.1_g000026 Rmu_sc0003317.1_g000014 Rmu_sc0003517.1_g000013 Rmu_sc0003543.1_g000011 Rmu_sc0003629.1_g000008 Rmu_sc0003731.1_g000005 Rmu_sc0004088.1_g000005 Rmu_sc0004088.1_g000006 Rmu_sc0004094.1_g000035 Rmu_sc0004324.1_g000006 Rmu_sc0005106.1_g000004 Rmu_sc0005198.1_g000006 Rmu_sc0005599.1_g000009 Rmu_sc0005762.1_g000008 Rmu_sc0006168.1_g000029 Rmu_sc0006273.1_g000014 Rmu_sc0006399.1_g000010 Rmu_sc0006632.1_g000010 Rmu_sc0006656.1_g000001 Rmu_sc0006875.1_g000002 Rmu_sc0007017.1_g000006 Rmu_sc0007034.1_g000017 Rmu_sc0007840.1_g000007 Rmu_sc0009945.1_g000003 Rmu_sc0010071.1_g000002 Rmu_sc0010503.1_g000005 Rmu_sc0011218.1_g000008 Rmu_sc0014846.1_g000007 Rmu_sc0015852.1_g000002 Rmu_sc0029270.1_g000003 Rmu_sc0036358.1_g000001 Rmu_ssc0000050.1_g000071 Rmu_ssc0000110.1_g000008 Rmu_ssc0000267.1_g000009
rosa_roxburghii Rroxscaffold_2G00100340 Rroxscaffold_2G00144280 Rroxscaffold_3G00221910
rosa_rugosa Rorug04G0175800
rosa_wichuraiana Rw0G012500 Rw0G013760 Rw0G014530 Rw0G021600 Rw2G019730 Rw2G045390 Rw3G001890 Rw3G025260 Rw3G029050 Rw4G000250 Rw5G014160 Rw5G031520 Rw5G036440 Rw6G023350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 610
AccII CGCG 3 cut(s) 421, 449, 499
AccIII TCCGGA 1 cut(s) 267
AciI CCGC 3 cut(s) 180, 526, 723
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 2 cut(s) 177, 510
AcyI GRCGYC 1 cut(s) 704
AfaI GTAC 1 cut(s) 573
AfiI CCNNNNNNNGG 2 cut(s) 519, 633
AhlI ACTAGT 1 cut(s) 574
AjuI GAANNNNNNNTTGG 2 cut(s) 337, 369
AluBI AGCT 2 cut(s) 337, 711
AluI AGCT 2 cut(s) 337, 711
Alw26I GTCTC 1 cut(s) 609
AlwI GGATC 1 cut(s) 19
Aor13HI TCCGGA 1 cut(s) 267
AoxI GGCC 4 cut(s) 177, 377, 510, 521
ApaI GGGCCC 2 cut(s) 381, 525
ArsI GACNNNNNNTTYG 2 cut(s) 689, 721
AspLEI GCGC 4 cut(s) 423, 451, 490, 501
AspS9I GGNCC 5 cut(s) 94, 377, 378, 521, 522
AsuHPI GGTGA 3 cut(s) 11, 194, 756
AvaII GGWCC 1 cut(s) 94
BaeGI GKGCMC 2 cut(s) 381, 525
BanII GRGCYC 2 cut(s) 381, 525
BbvCI CCTCAGC 1 cut(s) 359
BccI CCATC 3 cut(s) 172, 283, 694
BceAI ACGGC 1 cut(s) 576
BcoDI GTCTC 1 cut(s) 609
BcuI ACTAGT 1 cut(s) 574
BfaI CTAG 1 cut(s) 575
BfoI RGCGCY 1 cut(s) 491
BglI GCCNNNNNGGC 3 cut(s) 77, 253, 355
BisI GCNGC 2 cut(s) 180, 724
BlsI GCNGC 2 cut(s) 181, 725
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 5 cut(s) 94, 377, 378, 521, 522
BmiI GGNNCC 4 cut(s) 379, 380, 523, 524
BmrI ACTGGG 2 cut(s) 376, 528
BmsI GCATC 2 cut(s) 25, 89
BmuI ACTGGG 2 cut(s) 376, 528
BpmI CTGGAG 1 cut(s) 335
Bpu10I CCTNAGC 1 cut(s) 359
BsaHI GRCGYC 1 cut(s) 704
BsaJI CCNNGG 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 267
BsaXI ACNNNNNCTCC 4 cut(s) 592, 622, 625, 655
Bsc4I CCNNNNNNNGG 2 cut(s) 519, 633
Bse118I RCCGGY 1 cut(s) 686
Bse1I ACTGG 4 cut(s) 97, 382, 523, 529
BseAI TCCGGA 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 72
BseLI CCNNNNNNNGG 2 cut(s) 519, 633
BseMII CTCAG 1 cut(s) 350
BseNI ACTGG 4 cut(s) 97, 382, 523, 529
BseSI GKGCMC 2 cut(s) 381, 525
BseX3I CGGCCG 1 cut(s) 177
BseYI CCCAGC 1 cut(s) 374
Bsh1236I CGCG 3 cut(s) 421, 449, 499
Bsh1285I CGRYCG 2 cut(s) 180, 668
BshFI GGCC 4 cut(s) 179, 379, 512, 523
BsiEI CGRYCG 2 cut(s) 180, 668
BsiSI CCGG 4 cut(s) 28, 268, 631, 687
BslI CCNNNNNNNGG 2 cut(s) 519, 633
BsmAI GTCTC 1 cut(s) 609
BsnI GGCC 4 cut(s) 179, 379, 512, 523
Bsp120I GGGCCC 2 cut(s) 377, 521
Bsp1286I GDGCHC 2 cut(s) 381, 525
Bsp13I TCCGGA 1 cut(s) 267
Bsp143I GATC 2 cut(s) 24, 233
BspACI CCGC 3 cut(s) 180, 526, 723
BspANI GGCC 4 cut(s) 179, 379, 512, 523
BspCNI CTCAG 1 cut(s) 351
BspEI TCCGGA 1 cut(s) 267
BspFNI CGCG 3 cut(s) 421, 449, 499
BspLI GGNNCC 4 cut(s) 379, 380, 523, 524
BspPI GGATC 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 686
BsrI ACTGG 4 cut(s) 97, 382, 523, 529
BssAI RCCGGY 1 cut(s) 686
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 2 cut(s) 24, 233
BssNAI GTATAC 1 cut(s) 611
BssNI GRCGYC 1 cut(s) 704
BssT1I CCWWGG 1 cut(s) 72
Bst1107I GTATAC 1 cut(s) 611
Bst4CI ACNGT 2 cut(s) 188, 666
Bst6I CTCTTC 1 cut(s) 110
BstACI GRCGYC 1 cut(s) 704
BstC8I GCNNGC 3 cut(s) 624, 628, 692
BstDEI CTNAG 3 cut(s) 292, 359, 566
BstENI CCTNNNNNAGG 1 cut(s) 631
BstFNI CGCG 3 cut(s) 421, 449, 499
BstH2I RGCGCY 1 cut(s) 491
BstHHI GCGC 4 cut(s) 423, 451, 490, 501
BstKTI GATC 2 cut(s) 27, 236
BstMAI GTCTC 1 cut(s) 609
BstMBI GATC 2 cut(s) 24, 233
BstMCI CGRYCG 2 cut(s) 180, 668
BstMWI GCNNNNNNNGC 6 cut(s) 68, 77, 253, 355, 385, 496
BstNSI RCATGY 2 cut(s) 216, 694
BstSLI GKGCMC 2 cut(s) 381, 525
BstUI CGCG 3 cut(s) 421, 449, 499
BstZ17I GTATAC 1 cut(s) 611
BstZI CGGCCG 1 cut(s) 177
BsuRI GGCC 4 cut(s) 179, 379, 512, 523
BtsIMutI CAGTG 2 cut(s) 516, 536
Cac8I GCNNGC 3 cut(s) 624, 628, 692
CfoI GCGC 4 cut(s) 423, 451, 490, 501
Cfr10I RCCGGY 1 cut(s) 686
Cfr13I GGNCC 5 cut(s) 94, 377, 378, 521, 522
CseI GACGC 1 cut(s) 693
Csp6I GTAC 1 cut(s) 572
CviAII CATG 3 cut(s) 213, 391, 691
CviQI GTAC 1 cut(s) 572
DdeI CTNAG 3 cut(s) 292, 359, 566
DpnI GATC 2 cut(s) 26, 235
DpnII GATC 2 cut(s) 24, 233
EaeI YGGCCR 2 cut(s) 177, 510
EagI CGGCCG 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EclXI CGGCCG 1 cut(s) 177
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 2 cut(s) 381, 525
Eco47I GGWCC 1 cut(s) 94
Eco52I CGGCCG 1 cut(s) 177
EcoNI CCTNNNNNAGG 1 cut(s) 631
EcoO109I RGGNCCY 2 cut(s) 378, 522
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 2 cut(s) 381, 525
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 3 cut(s) 216, 394, 694
FaiI YATR 8 cut(s) 92, 170, 214, 352, 392, 493, 611, 692
FalI AAGNNNNNCTT 2 cut(s) 712, 744
FatI CATG 3 cut(s) 212, 390, 690
FauI CCCGC 1 cut(s) 533
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 2 cut(s) 180, 724
FriOI GRGCYC 2 cut(s) 381, 525
Fsp4HI GCNGC 2 cut(s) 180, 724
FspBI CTAG 1 cut(s) 575
GlaI GCGC 4 cut(s) 422, 450, 489, 500
GluI GCNGC 2 cut(s) 180, 724
GsaI CCCAGC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 335
HaeII RGCGCY 1 cut(s) 491
HaeIII GGCC 4 cut(s) 179, 379, 512, 523
HapII CCGG 4 cut(s) 28, 268, 631, 687
HgaI GACGC 1 cut(s) 693
HhaI GCGC 4 cut(s) 423, 451, 490, 501
Hin1I GRCGYC 1 cut(s) 704
Hin1II CATG 3 cut(s) 216, 394, 694
Hin6I GCGC 4 cut(s) 421, 449, 488, 499
HinP1I GCGC 4 cut(s) 421, 449, 488, 499
HinfI GANTC 5 cut(s) 225, 317, 368, 430, 436
HpaII CCGG 4 cut(s) 28, 268, 631, 687
HphI GGTGA 3 cut(s) 11, 194, 756
Hpy166II GTNNAC 1 cut(s) 611
Hpy188I TCNGA 1 cut(s) 435
Hpy188III TCNNGA 3 cut(s) 268, 314, 580
Hpy8I GTNNAC 1 cut(s) 611
Hpy99I CGWCG 2 cut(s) 562, 762
HpyAV CCTTC 4 cut(s) 209, 324, 358, 648
HpyCH4III ACNGT 2 cut(s) 188, 666
HpyCH4IV ACGT 1 cut(s) 467
HpyCH4V TGCA 3 cut(s) 16, 388, 622
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 77, 253, 355, 385, 496
HpyF3I CTNAG 3 cut(s) 292, 359, 566
HpySE526I ACGT 1 cut(s) 467
Hsp92I GRCGYC 1 cut(s) 704
Hsp92II CATG 3 cut(s) 216, 394, 694
HspAI GCGC 4 cut(s) 421, 449, 488, 499
Kpn2I TCCGGA 1 cut(s) 267
Kzo9I GATC 2 cut(s) 24, 233
LmnI GCTCC 3 cut(s) 91, 334, 600
LweI GCATC 2 cut(s) 25, 89
MaeI CTAG 1 cut(s) 575
MaeII ACGT 1 cut(s) 467
MaeIII GTNAC 1 cut(s) 660
MalI GATC 2 cut(s) 26, 235
MboI GATC 2 cut(s) 24, 233
MboII GAAGA 2 cut(s) 97, 723
MhlI GDGCHC 2 cut(s) 381, 525
MluCI AATT 1 cut(s) 592
MlyI GAGTC 2 cut(s) 326, 439
MmeI TCCRAC 3 cut(s) 305, 578, 651
MnlI CCTC 9 cut(s) 70, 134, 354, 419, 451, 468, 627, 729, 755
MroI TCCGGA 1 cut(s) 267
MseI TTAA 2 cut(s) 119, 715
MspI CCGG 4 cut(s) 28, 268, 631, 687
MvnI CGCG 3 cut(s) 421, 449, 499
MwoI GCNNNNNNNGC 6 cut(s) 68, 77, 253, 355, 385, 496
NdeII GATC 2 cut(s) 24, 233
NlaIII CATG 3 cut(s) 216, 394, 694
NlaIV GGNNCC 4 cut(s) 379, 380, 523, 524
NmuCI GTSAC 1 cut(s) 660
NspI RCATGY 2 cut(s) 216, 694
PaeI GCATGC 1 cut(s) 694
PcsI WCGNNNNNNNCGW 1 cut(s) 757
PfeI GAWTC 3 cut(s) 225, 368, 436
PkrI GCNGC 2 cut(s) 181, 725
PleI GAGTC 2 cut(s) 325, 438
PpsI GAGTC 2 cut(s) 325, 438
PspFI CCCAGC 1 cut(s) 374
PspN4I GGNNCC 4 cut(s) 379, 380, 523, 524
PspOMI GGGCCC 2 cut(s) 377, 521
PspPI GGNCC 5 cut(s) 94, 377, 378, 521, 522
RsaI GTAC 1 cut(s) 573
RsaNI GTAC 1 cut(s) 572
SaqAI TTAA 2 cut(s) 119, 715
SatI GCNGC 2 cut(s) 180, 724
Sau3AI GATC 2 cut(s) 24, 233
Sau96I GGNCC 5 cut(s) 94, 377, 378, 521, 522
SchI GAGTC 2 cut(s) 326, 439
SduI GDGCHC 2 cut(s) 381, 525
SetI ASST 8 cut(s) 8, 24, 207, 339, 470, 638, 713, 766
SfaNI GCATC 2 cut(s) 25, 89
SinI GGWCC 1 cut(s) 94
SpeI ACTAGT 1 cut(s) 574
SphI GCATGC 1 cut(s) 694
Sse9I AATT 1 cut(s) 592
SsiI CCGC 3 cut(s) 180, 526, 723
SspMI CTAG 1 cut(s) 575
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 188, 666
TaiI ACGT 1 cut(s) 470
TaqI TCGA 2 cut(s) 228, 734
TasI AATT 1 cut(s) 592
TauI GCSGC 2 cut(s) 182, 726
TfiI GAWTC 3 cut(s) 225, 368, 436
Tru1I TTAA 2 cut(s) 119, 715
Tru9I TTAA 2 cut(s) 119, 715
TscAI CASTG 2 cut(s) 523, 536
TseFI GTSAC 1 cut(s) 660
Tsp45I GTSAC 1 cut(s) 660
TspRI CASTG 2 cut(s) 523, 536
VpaK11BI GGWCC 1 cut(s) 94
XagI CCTNNNNNAGG 1 cut(s) 631
XceI RCATGY 2 cut(s) 216, 694
XcmI CCANNNNNNNNNTGG 1 cut(s) 255
XmiI GTMKAC 1 cut(s) 610
XspI CTAG 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.