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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 271 |
| Acc36I | ACCTGC | 1 cut(s) | 271 |
| AccII | CGCG | 1 cut(s) | 204 |
| AciI | CCGC | 1 cut(s) | 155 |
| AclWI | GGATC | 4 cut(s) | 281, 294, 386, 431 |
| AcuI | CTGAAG | 1 cut(s) | 426 |
| AdeI | CACNNNGTG | 1 cut(s) | 446 |
| AfaI | GTAC | 1 cut(s) | 209 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 407 |
| AgsI | TTSAA | 2 cut(s) | 173, 369 |
| AleI | CACNNNNGTG | 1 cut(s) | 18 |
| AluBI | AGCT | 2 cut(s) | 76, 116 |
| AluI | AGCT | 2 cut(s) | 76, 116 |
| Alw26I | GTCTC | 1 cut(s) | 405 |
| AlwI | GGATC | 4 cut(s) | 281, 294, 386, 431 |
| AoxI | GGCC | 1 cut(s) | 160 |
| AsuHPI | GGTGA | 2 cut(s) | 292, 418 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 191, 224 |
| BamHI | GGATCC | 1 cut(s) | 286 |
| BbvCI | CCTCAGC | 1 cut(s) | 261 |
| BccI | CCATC | 2 cut(s) | 152, 298 |
| BcoDI | GTCTC | 1 cut(s) | 405 |
| BfuAI | ACCTGC | 1 cut(s) | 271 |
| BmiI | GGNNCC | 1 cut(s) | 288 |
| BmsI | GCATC | 4 cut(s) | 142, 256, 267, 381 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 65, 97 |
| Bpu10I | CCTNAGC | 1 cut(s) | 261 |
| BpuEI | CTTGAG | 2 cut(s) | 87, 372 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 407 |
| BseGI | GGATG | 7 cut(s) | 49, 76, 97, 163, 271, 372, 465 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 407 |
| BseMII | CTCAG | 3 cut(s) | 57, 131, 252 |
| BseRI | GAGGAG | 1 cut(s) | 328 |
| Bsh1236I | CGCG | 1 cut(s) | 204 |
| BshFI | GGCC | 1 cut(s) | 162 |
| BslFI | GGGAC | 1 cut(s) | 108 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 407 |
| BsmAI | GTCTC | 1 cut(s) | 405 |
| BsmFI | GGGAC | 1 cut(s) | 108 |
| BsnI | GGCC | 1 cut(s) | 162 |
| Bsp143I | GATC | 3 cut(s) | 286, 391, 436 |
| BspACI | CCGC | 1 cut(s) | 155 |
| BspANI | GGCC | 1 cut(s) | 162 |
| BspCNI | CTCAG | 3 cut(s) | 58, 130, 253 |
| BspFNI | CGCG | 1 cut(s) | 204 |
| BspLI | GGNNCC | 1 cut(s) | 288 |
| BspMI | ACCTGC | 1 cut(s) | 271 |
| BspPI | GGATC | 4 cut(s) | 281, 294, 386, 431 |
| BssMI | GATC | 3 cut(s) | 286, 391, 436 |
| Bst4CI | ACNGT | 1 cut(s) | 228 |
| BstC8I | GCNNGC | 1 cut(s) | 271 |
| BstDEI | CTNAG | 3 cut(s) | 66, 117, 261 |
| BstF5I | GGATG | 7 cut(s) | 49, 76, 97, 163, 271, 372, 465 |
| BstFNI | CGCG | 1 cut(s) | 204 |
| BstKTI | GATC | 3 cut(s) | 289, 394, 439 |
| BstMAI | GTCTC | 1 cut(s) | 405 |
| BstMBI | GATC | 3 cut(s) | 286, 391, 436 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 266 |
| BstUI | CGCG | 1 cut(s) | 204 |
| BstX2I | RGATCY | 2 cut(s) | 286, 391 |
| BstYI | RGATCY | 2 cut(s) | 286, 391 |
| BsuRI | GGCC | 1 cut(s) | 162 |
| BtsCI | GGATG | 7 cut(s) | 49, 76, 97, 163, 271, 372, 465 |
| BveI | ACCTGC | 1 cut(s) | 271 |
| Cac8I | GCNNGC | 1 cut(s) | 271 |
| Csp6I | GTAC | 1 cut(s) | 208 |
| CviAII | CATG | 1 cut(s) | 32 |
| CviJI | RGCY | 4 cut(s) | 76, 116, 162, 423 |
| CviKI_1 | RGCY | 4 cut(s) | 76, 116, 162, 423 |
| CviQI | GTAC | 1 cut(s) | 208 |
| DdeI | CTNAG | 3 cut(s) | 66, 117, 261 |
| DpnI | GATC | 3 cut(s) | 288, 393, 438 |
| DpnII | GATC | 3 cut(s) | 286, 391, 436 |
| DraIII | CACNNNGTG | 1 cut(s) | 446 |
| Eco57I | CTGAAG | 1 cut(s) | 426 |
| FaeI | CATG | 1 cut(s) | 35 |
| FaiI | YATR | 3 cut(s) | 33, 189, 435 |
| FaqI | GGGAC | 1 cut(s) | 108 |
| FatI | CATG | 1 cut(s) | 31 |
| FokI | GGATG | 6 cut(s) | 56, 83, 84, 170, 278, 359 |
| HaeIII | GGCC | 1 cut(s) | 162 |
| Hin1II | CATG | 1 cut(s) | 35 |
| HincII | GTYRAC | 2 cut(s) | 37, 242 |
| HindII | GTYRAC | 2 cut(s) | 37, 242 |
| HphI | GGTGA | 2 cut(s) | 292, 418 |
| Hpy166II | GTNNAC | 2 cut(s) | 37, 242 |
| Hpy188I | TCNGA | 1 cut(s) | 120 |
| Hpy188III | TCNNGA | 2 cut(s) | 87, 389 |
| Hpy8I | GTNNAC | 2 cut(s) | 37, 242 |
| HpyAV | CCTTC | 2 cut(s) | 230, 401 |
| HpyCH4III | ACNGT | 1 cut(s) | 228 |
| HpyCH4IV | ACGT | 1 cut(s) | 256 |
| HpyCH4V | TGCA | 2 cut(s) | 178, 280 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 266 |
| HpyF3I | CTNAG | 3 cut(s) | 66, 117, 261 |
| HpySE526I | ACGT | 1 cut(s) | 256 |
| Hsp92II | CATG | 1 cut(s) | 35 |
| Kzo9I | GATC | 3 cut(s) | 286, 391, 436 |
| LpnPI | CCDG | 4 cut(s) | 266, 390, 405, 417 |
| LweI | GCATC | 4 cut(s) | 142, 256, 267, 381 |
| MaeII | ACGT | 1 cut(s) | 256 |
| MaeIII | GTNAC | 1 cut(s) | 448 |
| MalI | GATC | 3 cut(s) | 288, 393, 438 |
| MboI | GATC | 3 cut(s) | 286, 391, 436 |
| MflI | RGATCY | 2 cut(s) | 286, 391 |
| MluCI | AATT | 3 cut(s) | 246, 294, 345 |
| MnlI | CCTC | 8 cut(s) | 61, 91, 111, 226, 256, 306, 321, 349 |
| MslI | CAYNNNNRTG | 1 cut(s) | 18 |
| MvnI | CGCG | 1 cut(s) | 204 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 266 |
| NdeII | GATC | 3 cut(s) | 286, 391, 436 |
| NlaIII | CATG | 1 cut(s) | 35 |
| NlaIV | GGNNCC | 1 cut(s) | 288 |
| NmuCI | GTSAC | 1 cut(s) | 448 |
| OliI | CACNNNNGTG | 1 cut(s) | 18 |
| PaqCI | CACCTGC | 1 cut(s) | 271 |
| PspN4I | GGNNCC | 1 cut(s) | 288 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 46, 78 |
| PsuI | RGATCY | 2 cut(s) | 286, 391 |
| RsaI | GTAC | 1 cut(s) | 209 |
| RsaNI | GTAC | 1 cut(s) | 208 |
| RseI | CAYNNNNRTG | 1 cut(s) | 18 |
| Sau3AI | GATC | 3 cut(s) | 286, 391, 436 |
| SetI | ASST | 5 cut(s) | 78, 118, 241, 259, 285 |
| SfaNI | GCATC | 4 cut(s) | 142, 256, 267, 381 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 18 |
| SmlI | CTYRAG | 2 cut(s) | 102, 387 |
| SmoI | CTYRAG | 2 cut(s) | 102, 387 |
| Sse9I | AATT | 3 cut(s) | 246, 294, 345 |
| SsiI | CCGC | 1 cut(s) | 155 |
| SspI | AATATT | 1 cut(s) | 185 |
| TaaI | ACNGT | 1 cut(s) | 228 |
| TaiI | ACGT | 1 cut(s) | 259 |
| TasI | AATT | 3 cut(s) | 246, 294, 345 |
| TseFI | GTSAC | 1 cut(s) | 448 |
| Tsp45I | GTSAC | 1 cut(s) | 448 |
| TspDTI | ATGAA | 1 cut(s) | 204 |