Heat shock protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 527 |
| AccB1I | GGYRCC | 2 cut(s) | 317, 527 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 882 |
| AccBSI | CCGCTC | 1 cut(s) | 1335 |
| AccI | GTMKAC | 2 cut(s) | 1092, 2106 |
| AccII | CGCG | 1 cut(s) | 1496 |
| AciI | CCGC | 4 cut(s) | 5, 396, 1335, 1749 |
| AclWI | GGATC | 3 cut(s) | 593, 1772, 1961 |
| AcsI | RAATTY | 4 cut(s) | 150, 326, 614, 1989 |
| AcuI | CTGAAG | 2 cut(s) | 1376, 2024 |
| AfaI | GTAC | 4 cut(s) | 219, 301, 529, 1808 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 553, 882, 961, 1486, 1847 |
| AflIII | ACRYGT | 1 cut(s) | 1809 |
| AgsI | TTSAA | 6 cut(s) | 920, 937, 1153, 1198, 1261, 1525 |
| AjnI | CCWGG | 4 cut(s) | 42, 986, 1446, 1959 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1247, 1279 |
| Alw21I | GWGCWC | 3 cut(s) | 894, 1056, 1805 |
| Alw26I | GTCTC | 6 cut(s) | 23, 1555, 1580, 1696, 1925, 2027 |
| AlwI | GGATC | 3 cut(s) | 593, 1772, 1961 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 64, 1783, 1883 |
| AoxI | GGCC | 4 cut(s) | 228, 939, 1567, 1935 |
| ApeKI | GCWGC | 4 cut(s) | 787, 1719, 1918, 1982 |
| ApoI | RAATTY | 4 cut(s) | 150, 326, 614, 1989 |
| Asp718I | GGTACC | 1 cut(s) | 527 |
| AspS9I | GGNCC | 5 cut(s) | 556, 592, 1746, 1909, 1936 |
| AsuHPI | GGTGA | 6 cut(s) | 298, 521, 529, 1168, 1424, 1738 |
| AvaII | GGWCC | 4 cut(s) | 556, 592, 1746, 1909 |
| AxyI | CCTNAGG | 1 cut(s) | 828 |
| BaeGI | GKGCMC | 1 cut(s) | 320 |
| BanI | GGYRCC | 2 cut(s) | 317, 527 |
| BanII | GRGCYC | 3 cut(s) | 967, 1056, 1805 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1382, 1414 |
| BauI | CACGAG | 2 cut(s) | 596, 1122 |
| BbsI | GAAGAC | 4 cut(s) | 656, 687, 1284, 1829 |
| Bbv12I | GWGCWC | 3 cut(s) | 894, 1056, 1805 |
| BbvCI | CCTCAGC | 2 cut(s) | 1865, 2082 |
| BbvI | GCAGC | 4 cut(s) | 799, 1706, 1905, 1994 |
| BccI | CCATC | 6 cut(s) | 10, 254, 1529, 1658, 2069, 2089 |
| BceAI | ACGGC | 1 cut(s) | 1950 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2018, 2052 |
| BciT130I | CCWGG | 4 cut(s) | 44, 988, 1448, 1961 |
| BciVI | GTATCC | 1 cut(s) | 1280 |
| BclI | TGATCA | 2 cut(s) | 72, 1158 |
| BcoDI | GTCTC | 6 cut(s) | 23, 1555, 1580, 1696, 1925, 2027 |
| BfaI | CTAG | 2 cut(s) | 495, 1070 |
| BfmI | CTRYAG | 1 cut(s) | 88 |
| BfuI | GTATCC | 1 cut(s) | 1280 |
| BglII | AGATCT | 1 cut(s) | 1146 |
| BisI | GCNGC | 5 cut(s) | 788, 1336, 1720, 1919, 1983 |
| BlpI | GCTNAGC | 1 cut(s) | 2015 |
| BlsI | GCNGC | 5 cut(s) | 789, 1337, 1721, 1920, 1984 |
| Bme1390I | CCNGG | 4 cut(s) | 44, 988, 1448, 1961 |
| Bme18I | GGWCC | 4 cut(s) | 556, 592, 1746, 1909 |
| BmgT120I | GGNCC | 5 cut(s) | 556, 592, 1746, 1909, 1936 |
| BmiI | GGNNCC | 5 cut(s) | 319, 352, 481, 529, 966 |
| BmrFI | CCNGG | 4 cut(s) | 44, 988, 1448, 1961 |
| BmrI | ACTGGG | 1 cut(s) | 892 |
| BmsI | GCATC | 6 cut(s) | 13, 127, 178, 1119, 1989, 2038 |
| BmuI | ACTGGG | 1 cut(s) | 892 |
| BpiI | GAAGAC | 4 cut(s) | 656, 687, 1284, 1829 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1232, 1264 |
| BpmI | CTGGAG | 1 cut(s) | 369 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1776, 1865, 2082 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2015 |
| BpuEI | CTTGAG | 3 cut(s) | 533, 950, 1556 |
| BsaI | GGTCTC | 3 cut(s) | 23, 1555, 1696 |
| BsaJI | CCNNGG | 2 cut(s) | 321, 1560 |
| BsaWI | WCCGGW | 1 cut(s) | 1730 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 342, 372, 545, 575 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 553, 882, 961, 1486, 1847 |
| Bse1I | ACTGG | 3 cut(s) | 559, 887, 1414 |
| Bse21I | CCTNAGG | 1 cut(s) | 828 |
| BseBI | CCWGG | 4 cut(s) | 44, 988, 1448, 1961 |
| BseDI | CCNNGG | 2 cut(s) | 321, 1560 |
| BseGI | GGATG | 5 cut(s) | 505, 850, 1056, 1969, 2004 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 553, 882, 961, 1486, 1847 |
| BseNI | ACTGG | 3 cut(s) | 559, 887, 1414 |
| BseSI | GKGCMC | 1 cut(s) | 320 |
| BseXI | GCAGC | 4 cut(s) | 799, 1706, 1905, 1994 |
| BseYI | CCCAGC | 2 cut(s) | 2008, 2039 |
| Bsh1236I | CGCG | 1 cut(s) | 1496 |
| BshFI | GGCC | 4 cut(s) | 230, 941, 1569, 1937 |
| BshNI | GGYRCC | 2 cut(s) | 317, 527 |
| BsiHKAI | GWGCWC | 3 cut(s) | 894, 1056, 1805 |
| BsiSI | CCGG | 1 cut(s) | 1731 |
| BslFI | GGGAC | 2 cut(s) | 938, 1802 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 553, 882, 961, 1486, 1847 |
| BsmAI | GTCTC | 6 cut(s) | 23, 1555, 1580, 1696, 1925, 2027 |
| BsmBI | CGTCTC | 1 cut(s) | 1925 |
| BsmFI | GGGAC | 2 cut(s) | 938, 1802 |
| BsmI | GAATGC | 1 cut(s) | 1244 |
| BsnI | GGCC | 4 cut(s) | 230, 941, 1569, 1937 |
| Bso31I | GGTCTC | 3 cut(s) | 23, 1555, 1696 |
| Bsp1286I | GDGCHC | 5 cut(s) | 320, 894, 967, 1056, 1805 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2015 |
| BspACI | CCGC | 4 cut(s) | 5, 396, 1335, 1749 |
| BspANI | GGCC | 4 cut(s) | 230, 941, 1569, 1937 |
| BspFNI | CGCG | 1 cut(s) | 1496 |
| BspHI | TCATGA | 2 cut(s) | 778, 1767 |
| BspLI | GGNNCC | 5 cut(s) | 319, 352, 481, 529, 966 |
| BspPI | GGATC | 3 cut(s) | 593, 1772, 1961 |
| BspT107I | GGYRCC | 2 cut(s) | 317, 527 |
| BspTNI | GGTCTC | 3 cut(s) | 23, 1555, 1696 |
| BsrBI | CCGCTC | 1 cut(s) | 1335 |
| BsrI | ACTGG | 3 cut(s) | 559, 887, 1414 |
| BssECI | CCNNGG | 2 cut(s) | 321, 1560 |
| BssSI | CACGAG | 2 cut(s) | 596, 1122 |
| BssT1I | CCWWGG | 2 cut(s) | 321, 1560 |
| Bst2BI | CACGAG | 2 cut(s) | 596, 1122 |
| Bst2UI | CCWGG | 4 cut(s) | 44, 988, 1448, 1961 |
| Bst4CI | ACNGT | 4 cut(s) | 257, 375, 490, 1046 |
| Bst6I | CTCTTC | 5 cut(s) | 548, 570, 696, 1590, 1865 |
| BstC8I | GCNNGC | 5 cut(s) | 182, 345, 824, 1302, 2061 |
| BstEII | GGTNACC | 1 cut(s) | 1726 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 551 |
| BstF5I | GGATG | 5 cut(s) | 505, 850, 1056, 1969, 2004 |
| BstFNI | CGCG | 1 cut(s) | 1496 |
| BstMAI | GTCTC | 6 cut(s) | 23, 1555, 1580, 1696, 1925, 2027 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 44, 344, 823, 1301 |
| BstNI | CCWGG | 4 cut(s) | 44, 988, 1448, 1961 |
| BstNSI | RCATGY | 2 cut(s) | 1813, 2059 |
| BstPI | GGTNACC | 1 cut(s) | 1726 |
| BstSCI | CCNGG | 4 cut(s) | 42, 986, 1446, 1959 |
| BstSFI | CTRYAG | 1 cut(s) | 88 |
| BstSLI | GKGCMC | 1 cut(s) | 320 |
| BstUI | CGCG | 1 cut(s) | 1496 |
| BstV1I | GCAGC | 4 cut(s) | 799, 1706, 1905, 1994 |
| BstV2I | GAAGAC | 4 cut(s) | 656, 687, 1284, 1829 |
| BstX2I | RGATCY | 1 cut(s) | 1146 |
| BstYI | RGATCY | 1 cut(s) | 1146 |
| Bsu36I | CCTNAGG | 1 cut(s) | 828 |
| BsuI | GTATCC | 1 cut(s) | 1280 |
| BsuRI | GGCC | 4 cut(s) | 230, 941, 1569, 1937 |
| BtsCI | GGATG | 5 cut(s) | 505, 850, 1056, 1969, 2004 |
| BtsI | GCAGTG | 1 cut(s) | 1434 |
| BtsIMutI | CAGTG | 5 cut(s) | 262, 486, 676, 1407, 1434 |
| Cac8I | GCNNGC | 5 cut(s) | 182, 345, 824, 1302, 2061 |
| CaiI | CAGNNNCTG | 3 cut(s) | 64, 1783, 1883 |
| CciI | TCATGA | 2 cut(s) | 778, 1767 |
| Cfr13I | GGNCC | 5 cut(s) | 556, 592, 1746, 1909, 1936 |
| CseI | GACGC | 2 cut(s) | 247, 1502 |
| Csp6I | GTAC | 4 cut(s) | 218, 300, 528, 1807 |
| CviQI | GTAC | 4 cut(s) | 218, 300, 528, 1807 |
| Eam1104I | CTCTTC | 5 cut(s) | 548, 570, 696, 1590, 1865 |
| EarI | CTCTTC | 5 cut(s) | 548, 570, 696, 1590, 1865 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Ecl136II | GAGCTC | 2 cut(s) | 1054, 1803 |
| Eco130I | CCWWGG | 2 cut(s) | 321, 1560 |
| Eco147I | AGGCCT | 1 cut(s) | 1569 |
| Eco24I | GRGCYC | 3 cut(s) | 967, 1056, 1805 |
| Eco31I | GGTCTC | 3 cut(s) | 23, 1555, 1696 |
| Eco47I | GGWCC | 4 cut(s) | 556, 592, 1746, 1909 |
| Eco53kI | GAGCTC | 2 cut(s) | 1054, 1803 |
| Eco57I | CTGAAG | 2 cut(s) | 1376, 2024 |
| Eco81I | CCTNAGG | 1 cut(s) | 828 |
| Eco91I | GGTNACC | 1 cut(s) | 1726 |
| EcoICRI | GAGCTC | 2 cut(s) | 1054, 1803 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 551 |
| EcoO109I | RGGNCCY | 2 cut(s) | 592, 1909 |
| EcoO65I | GGTNACC | 1 cut(s) | 1726 |
| EcoRI | GAATTC | 2 cut(s) | 614, 1989 |
| EcoRII | CCWGG | 4 cut(s) | 42, 986, 1446, 1959 |
| EcoT14I | CCWWGG | 2 cut(s) | 321, 1560 |
| EcoT38I | GRGCYC | 3 cut(s) | 967, 1056, 1805 |
| ErhI | CCWWGG | 2 cut(s) | 321, 1560 |
| Esp3I | CGTCTC | 1 cut(s) | 1925 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 912, 944 |
| FaqI | GGGAC | 2 cut(s) | 938, 1802 |
| FbaI | TGATCA | 2 cut(s) | 72, 1158 |
| FblI | GTMKAC | 2 cut(s) | 1092, 2106 |
| Fnu4HI | GCNGC | 5 cut(s) | 788, 1336, 1720, 1919, 1983 |
| FokI | GGATG | 5 cut(s) | 512, 857, 1043, 1976, 2011 |
| FriOI | GRGCYC | 3 cut(s) | 967, 1056, 1805 |
| Fsp4HI | GCNGC | 5 cut(s) | 788, 1336, 1720, 1919, 1983 |
| FspBI | CTAG | 2 cut(s) | 495, 1070 |
| GluI | GCNGC | 5 cut(s) | 788, 1336, 1720, 1919, 1983 |
| GsaI | CCCAGC | 2 cut(s) | 2012, 2043 |
| GsuI | CTGGAG | 1 cut(s) | 369 |
| HaeIII | GGCC | 4 cut(s) | 230, 941, 1569, 1937 |
| HapII | CCGG | 1 cut(s) | 1731 |
| HgaI | GACGC | 2 cut(s) | 247, 1502 |
| HincII | GTYRAC | 3 cut(s) | 1347, 1944, 2107 |
| HindII | GTYRAC | 3 cut(s) | 1347, 1944, 2107 |
| HindIII | AAGCTT | 3 cut(s) | 1010, 1298, 1574 |
| HinfI | GANTC | 6 cut(s) | 464, 1094, 1168, 1317, 1709, 1851 |
| HpaII | CCGG | 1 cut(s) | 1731 |
| HphI | GGTGA | 6 cut(s) | 298, 521, 529, 1168, 1424, 1738 |
| Hpy166II | GTNNAC | 8 cut(s) | 289, 512, 1093, 1347, 1492, 1708, 1944, 2107 |
| Hpy8I | GTNNAC | 8 cut(s) | 289, 512, 1093, 1347, 1492, 1708, 1944, 2107 |
| Hpy99I | CGWCG | 1 cut(s) | 689 |
| HpyCH4III | ACNGT | 4 cut(s) | 257, 375, 490, 1046 |
| HpyCH4IV | ACGT | 1 cut(s) | 9 |
| HpyCH4V | TGCA | 7 cut(s) | 26, 308, 343, 1556, 1644, 1982, 2063 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 44, 344, 823, 1301 |
| HpySE526I | ACGT | 1 cut(s) | 9 |
| KpnI | GGTACC | 1 cut(s) | 531 |
| Ksp22I | TGATCA | 2 cut(s) | 72, 1158 |
| LmnI | GCTCC | 5 cut(s) | 350, 889, 970, 1051, 1861 |
| Lsp1109I | GCAGC | 4 cut(s) | 799, 1706, 1905, 1994 |
| LweI | GCATC | 6 cut(s) | 13, 127, 178, 1119, 1989, 2038 |
| MaeI | CTAG | 2 cut(s) | 495, 1070 |
| MaeII | ACGT | 1 cut(s) | 9 |
| MaeIII | GTNAC | 4 cut(s) | 424, 490, 1366, 1726 |
| MbiI | CCGCTC | 1 cut(s) | 1335 |
| MfeI | CAATTG | 2 cut(s) | 303, 1520 |
| MflI | RGATCY | 1 cut(s) | 1146 |
| MhlI | GDGCHC | 5 cut(s) | 320, 894, 967, 1056, 1805 |
| MlyI | GAGTC | 2 cut(s) | 1088, 1703 |
| MmeI | TCCRAC | 1 cut(s) | 543 |
| MseI | TTAA | 1 cut(s) | 1008 |
| MslI | CAYNNNNRTG | 1 cut(s) | 441 |
| MspI | CCGG | 1 cut(s) | 1731 |
| MspR9I | CCNGG | 4 cut(s) | 44, 988, 1448, 1961 |
| MunI | CAATTG | 2 cut(s) | 303, 1520 |
| Mva1269I | GAATGC | 1 cut(s) | 1244 |
| MvaI | CCWGG | 4 cut(s) | 44, 988, 1448, 1961 |
| MvnI | CGCG | 1 cut(s) | 1496 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 44, 344, 823, 1301 |
| NlaIV | GGNNCC | 5 cut(s) | 319, 352, 481, 529, 966 |
| NmuCI | GTSAC | 4 cut(s) | 424, 490, 1366, 1726 |
| NspI | RCATGY | 2 cut(s) | 1813, 2059 |
| PagI | TCATGA | 2 cut(s) | 778, 1767 |
| PceI | AGGCCT | 1 cut(s) | 1569 |
| PciI | ACATGT | 1 cut(s) | 1809 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1500 |
| PctI | GAATGC | 1 cut(s) | 1244 |
| PfeI | GAWTC | 4 cut(s) | 464, 1168, 1317, 1851 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 882 |
| PfoI | TCCNGGA | 1 cut(s) | 1446 |
| PkrI | GCNGC | 5 cut(s) | 789, 1337, 1721, 1920, 1984 |
| PleI | GAGTC | 2 cut(s) | 1088, 1703 |
| PpsI | GAGTC | 2 cut(s) | 1088, 1703 |
| PpuMI | RGGWCCY | 2 cut(s) | 592, 1909 |
| PscI | ACATGT | 1 cut(s) | 1809 |
| Psp124BI | GAGCTC | 2 cut(s) | 1056, 1805 |
| Psp5II | RGGWCCY | 2 cut(s) | 592, 1909 |
| Psp6I | CCWGG | 4 cut(s) | 42, 986, 1446, 1959 |
| PspEI | GGTNACC | 1 cut(s) | 1726 |
| PspFI | CCCAGC | 2 cut(s) | 2008, 2039 |
| PspGI | CCWGG | 4 cut(s) | 42, 986, 1446, 1959 |
| PspN4I | GGNNCC | 5 cut(s) | 319, 352, 481, 529, 966 |
| PspPI | GGNCC | 5 cut(s) | 556, 592, 1746, 1909, 1936 |
| PspPPI | RGGWCCY | 2 cut(s) | 592, 1909 |
| PstNI | CAGNNNCTG | 3 cut(s) | 64, 1783, 1883 |
| PsuI | RGATCY | 1 cut(s) | 1146 |
| RsaI | GTAC | 4 cut(s) | 219, 301, 529, 1808 |
| RsaNI | GTAC | 4 cut(s) | 218, 300, 528, 1807 |
| RseI | CAYNNNNRTG | 1 cut(s) | 441 |
| SacI | GAGCTC | 2 cut(s) | 1056, 1805 |
| SalI | GTCGAC | 1 cut(s) | 2105 |
| SaqAI | TTAA | 1 cut(s) | 1008 |
| SatI | GCNGC | 5 cut(s) | 788, 1336, 1720, 1919, 1983 |
| Sau96I | GGNCC | 5 cut(s) | 556, 592, 1746, 1909, 1936 |
| SchI | GAGTC | 2 cut(s) | 1088, 1703 |
| ScrFI | CCNGG | 4 cut(s) | 44, 988, 1448, 1961 |
| SduI | GDGCHC | 5 cut(s) | 320, 894, 967, 1056, 1805 |
| SfaNI | GCATC | 6 cut(s) | 13, 127, 178, 1119, 1989, 2038 |
| SfcI | CTRYAG | 1 cut(s) | 88 |
| SinI | GGWCC | 4 cut(s) | 556, 592, 1746, 1909 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 441 |
| SmlI | CTYRAG | 3 cut(s) | 548, 929, 1571 |
| SmoI | CTYRAG | 3 cut(s) | 548, 929, 1571 |
| SseBI | AGGCCT | 1 cut(s) | 1569 |
| SsiI | CCGC | 4 cut(s) | 5, 396, 1335, 1749 |
| SspMI | CTAG | 2 cut(s) | 495, 1070 |
| SstI | GAGCTC | 2 cut(s) | 1056, 1805 |
| StuI | AGGCCT | 1 cut(s) | 1569 |
| StyD4I | CCNGG | 4 cut(s) | 42, 986, 1446, 1959 |
| StyI | CCWWGG | 2 cut(s) | 321, 1560 |
| TaaI | ACNGT | 4 cut(s) | 257, 375, 490, 1046 |
| TaiI | ACGT | 1 cut(s) | 12 |
| TaqI | TCGA | 6 cut(s) | 112, 154, 186, 1188, 1929, 2106 |
| TauI | GCSGC | 1 cut(s) | 1338 |
| TfiI | GAWTC | 4 cut(s) | 464, 1168, 1317, 1851 |
| Tru1I | TTAA | 1 cut(s) | 1008 |
| Tru9I | TTAA | 1 cut(s) | 1008 |
| TscAI | CASTG | 5 cut(s) | 262, 493, 676, 1414, 1434 |
| TseFI | GTSAC | 4 cut(s) | 424, 490, 1366, 1726 |
| TseI | GCWGC | 4 cut(s) | 787, 1719, 1918, 1982 |
| Tsp45I | GTSAC | 4 cut(s) | 424, 490, 1366, 1726 |
| TspGWI | ACGGA | 3 cut(s) | 185, 1431, 1863 |
| TspRI | CASTG | 5 cut(s) | 262, 493, 676, 1414, 1434 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 882 |
| VpaK11BI | GGWCC | 4 cut(s) | 556, 592, 1746, 1909 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 551 |
| XapI | RAATTY | 4 cut(s) | 150, 326, 614, 1989 |
| XceI | RCATGY | 2 cut(s) | 1813, 2059 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 280, 328 |
| XmiI | GTMKAC | 2 cut(s) | 1092, 2106 |
| XspI | CTAG | 2 cut(s) | 495, 1070 |