Heat shock protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 563, 989 |
| Acc16I | TGCGCA | 1 cut(s) | 977 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 837 |
| AccBSI | CCGCTC | 2 cut(s) | 1066, 1856 |
| AccI | GTMKAC | 2 cut(s) | 1178, 1977 |
| AccII | CGCG | 2 cut(s) | 102, 1078 |
| AccIII | TCCGGA | 1 cut(s) | 153 |
| AciI | CCGC | 4 cut(s) | 35, 100, 1064, 1854 |
| AclWI | GGATC | 2 cut(s) | 164, 1880 |
| AcsI | RAATTY | 1 cut(s) | 1184 |
| AcuI | CTGAAG | 1 cut(s) | 1508 |
| AfaI | GTAC | 4 cut(s) | 732, 1022, 1271, 1595 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 169, 837, 1216, 1243 |
| AflII | CTTAAG | 2 cut(s) | 530, 1409 |
| AflIII | ACRYGT | 1 cut(s) | 1728 |
| AgeI | ACCGGT | 1 cut(s) | 1361 |
| AgsI | TTSAA | 3 cut(s) | 892, 1153, 1477 |
| AhlI | ACTAGT | 1 cut(s) | 1963 |
| AjiI | CACGTC | 1 cut(s) | 301 |
| AjnI | CCWGG | 1 cut(s) | 30 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1199, 1231 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 135, 167 |
| Alw21I | GWGCWC | 2 cut(s) | 108, 922 |
| Alw26I | GTCTC | 6 cut(s) | 11, 138, 414, 1793, 1844, 1937 |
| AlwI | GGATC | 2 cut(s) | 164, 1880 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 52 |
| Ama87I | CYCGRG | 3 cut(s) | 128, 884, 1142 |
| Aor13HI | TCCGGA | 1 cut(s) | 153 |
| AoxI | GGCC | 3 cut(s) | 288, 877, 1336 |
| ApeKI | GCWGC | 3 cut(s) | 1087, 1837, 1840 |
| ApoI | RAATTY | 1 cut(s) | 1184 |
| AseI | ATTAAT | 1 cut(s) | 1755 |
| AsiGI | ACCGGT | 1 cut(s) | 1361 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 863, 1521 |
| AspLEI | GCGC | 2 cut(s) | 978, 1630 |
| AspS9I | GGNCC | 4 cut(s) | 255, 261, 471, 1336 |
| AsuC2I | CCSGG | 2 cut(s) | 259, 292 |
| AsuHPI | GGTGA | 9 cut(s) | 178, 241, 490, 671, 730, 1123, 1141, 1376, 1834 |
| AsuII | TTCGAA | 1 cut(s) | 794 |
| AvaI | CYCGRG | 3 cut(s) | 128, 884, 1142 |
| AvaII | GGWCC | 3 cut(s) | 255, 261, 471 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 722, 755 |
| BanII | GRGCYC | 1 cut(s) | 108 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1334, 1366 |
| BauI | CACGAG | 2 cut(s) | 940, 1321 |
| BbsI | GAAGAC | 4 cut(s) | 504, 849, 1607, 1748 |
| Bbv12I | GWGCWC | 2 cut(s) | 108, 922 |
| BbvCI | CCTCAGC | 2 cut(s) | 773, 1784 |
| BbvI | GCAGC | 3 cut(s) | 1074, 1824, 1827 |
| BccI | CCATC | 4 cut(s) | 197, 715, 1481, 2014 |
| BciT130I | CCWGG | 1 cut(s) | 32 |
| BciVI | GTATCC | 1 cut(s) | 1232 |
| BclI | TGATCA | 2 cut(s) | 60, 391 |
| BcnI | CCSGG | 2 cut(s) | 259, 292 |
| BcoDI | GTCTC | 6 cut(s) | 11, 138, 414, 1793, 1844, 1937 |
| BcuI | ACTAGT | 1 cut(s) | 1963 |
| BfaI | CTAG | 3 cut(s) | 438, 1734, 1964 |
| BfmI | CTRYAG | 2 cut(s) | 76, 1770 |
| BfrI | CTTAAG | 2 cut(s) | 530, 1409 |
| BfuI | GTATCC | 1 cut(s) | 1232 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 769 |
| BisI | GCNGC | 4 cut(s) | 1064, 1088, 1838, 1841 |
| BlsI | GCNGC | 4 cut(s) | 1065, 1089, 1839, 1842 |
| Bme1390I | CCNGG | 3 cut(s) | 32, 259, 292 |
| Bme18I | GGWCC | 3 cut(s) | 255, 261, 471 |
| BmeT110I | CYCGRG | 3 cut(s) | 128, 884, 1142 |
| BmgBI | CACGTC | 1 cut(s) | 301 |
| BmgT120I | GGNCC | 4 cut(s) | 255, 261, 471, 1336 |
| BmiI | GGNNCC | 2 cut(s) | 262, 472 |
| BmrFI | CCNGG | 3 cut(s) | 32, 259, 292 |
| BmrI | ACTGGG | 1 cut(s) | 1937 |
| BmsI | GCATC | 5 cut(s) | 159, 619, 1002, 1074, 1908 |
| BmuI | ACTGGG | 1 cut(s) | 1937 |
| BpiI | GAAGAC | 4 cut(s) | 504, 849, 1607, 1748 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 794, 826, 1184, 1216 |
| BpmI | CTGGAG | 1 cut(s) | 1421 |
| Bpu10I | CCTNAGC | 5 cut(s) | 773, 1265, 1499, 1695, 1784 |
| Bpu14I | TTCGAA | 1 cut(s) | 794 |
| BpuEI | CTTGAG | 4 cut(s) | 476, 1091, 1196, 1550 |
| BpuMI | CCSGG | 2 cut(s) | 259, 292 |
| BsaBI | GATNNNNATC | 1 cut(s) | 390 |
| BsaI | GGTCTC | 3 cut(s) | 11, 1844, 1937 |
| BsaJI | CCNNGG | 5 cut(s) | 291, 474, 597, 1024, 1605 |
| BsaWI | WCCGGW | 2 cut(s) | 153, 1361 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 169, 837, 1216, 1243 |
| Bse118I | RCCGGY | 2 cut(s) | 200, 1361 |
| Bse1I | ACTGG | 1 cut(s) | 1932 |
| Bse3DI | GCAATG | 1 cut(s) | 121 |
| Bse8I | GATNNNNATC | 1 cut(s) | 390 |
| BseAI | TCCGGA | 1 cut(s) | 153 |
| BseBI | CCWGG | 1 cut(s) | 32 |
| BseDI | CCNNGG | 5 cut(s) | 291, 474, 597, 1024, 1605 |
| BseGI | GGATG | 6 cut(s) | 150, 159, 448, 1332, 1540, 1923 |
| BseJI | GATNNNNATC | 1 cut(s) | 390 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 169, 837, 1216, 1243 |
| BseMI | GCAATG | 1 cut(s) | 121 |
| BseMII | CTCAG | 9 cut(s) | 44, 345, 425, 546, 764, 1279, 1709, 1775, 1948 |
| BseNI | ACTGG | 1 cut(s) | 1932 |
| BseRI | GAGGAG | 8 cut(s) | 668, 800, 815, 1019, 1562, 1580, 1793, 2009 |
| BseXI | GCAGC | 3 cut(s) | 1074, 1824, 1827 |
| BseYI | CCCAGC | 1 cut(s) | 1901 |
| Bsh1236I | CGCG | 2 cut(s) | 102, 1078 |
| BshFI | GGCC | 3 cut(s) | 290, 879, 1338 |
| BshTI | ACCGGT | 1 cut(s) | 1361 |
| BsiHKAI | GWGCWC | 2 cut(s) | 108, 922 |
| BsiHKCI | CYCGRG | 3 cut(s) | 128, 884, 1142 |
| BsiSI | CCGG | 7 cut(s) | 154, 201, 258, 291, 782, 1016, 1362 |
| BslFI | GGGAC | 4 cut(s) | 256, 274, 484, 1721 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 169, 837, 1216, 1243 |
| BsmAI | GTCTC | 6 cut(s) | 11, 138, 414, 1793, 1844, 1937 |
| BsmFI | GGGAC | 4 cut(s) | 256, 274, 484, 1721 |
| BsmI | GAATGC | 2 cut(s) | 26, 550 |
| BsnI | GGCC | 3 cut(s) | 290, 879, 1338 |
| Bso31I | GGTCTC | 3 cut(s) | 11, 1844, 1937 |
| BsoBI | CYCGRG | 3 cut(s) | 128, 884, 1142 |
| Bsp119I | TTCGAA | 1 cut(s) | 794 |
| Bsp1286I | GDGCHC | 2 cut(s) | 108, 922 |
| Bsp13I | TCCGGA | 1 cut(s) | 153 |
| Bsp1407I | TGTACA | 1 cut(s) | 1593 |
| Bsp143I | GATC | 9 cut(s) | 39, 60, 124, 156, 309, 391, 928, 1057, 1885 |
| BspACI | CCGC | 4 cut(s) | 35, 100, 1064, 1854 |
| BspANI | GGCC | 3 cut(s) | 290, 879, 1338 |
| BspCNI | CTCAG | 9 cut(s) | 45, 346, 424, 547, 765, 1278, 1708, 1776, 1947 |
| BspEI | TCCGGA | 1 cut(s) | 153 |
| BspFNI | CGCG | 2 cut(s) | 102, 1078 |
| BspLI | GGNNCC | 2 cut(s) | 262, 472 |
| BspPI | GGATC | 2 cut(s) | 164, 1880 |
| BspT104I | TTCGAA | 1 cut(s) | 794 |
| BspTI | CTTAAG | 2 cut(s) | 530, 1409 |
| BspTNI | GGTCTC | 3 cut(s) | 11, 1844, 1937 |
| BsrBI | CCGCTC | 2 cut(s) | 1066, 1856 |
| BsrDI | GCAATG | 1 cut(s) | 121 |
| BsrFI | RCCGGY | 2 cut(s) | 200, 1361 |
| BsrGI | TGTACA | 1 cut(s) | 1593 |
| BsrI | ACTGG | 1 cut(s) | 1932 |
| BssAI | RCCGGY | 2 cut(s) | 200, 1361 |
| BssECI | CCNNGG | 5 cut(s) | 291, 474, 597, 1024, 1605 |
| BssMI | GATC | 9 cut(s) | 39, 60, 124, 156, 309, 391, 928, 1057, 1885 |
| BssNAI | GTATAC | 1 cut(s) | 1179 |
| BssSI | CACGAG | 2 cut(s) | 940, 1321 |
| BssT1I | CCWWGG | 3 cut(s) | 474, 1024, 1605 |
| Bst1107I | GTATAC | 1 cut(s) | 1179 |
| Bst2BI | CACGAG | 2 cut(s) | 940, 1321 |
| Bst2UI | CCWGG | 1 cut(s) | 32 |
| Bst4CI | ACNGT | 3 cut(s) | 433, 598, 1473 |
| Bst6I | CTCTTC | 3 cut(s) | 908, 1539, 1784 |
| BstAFI | CTTAAG | 2 cut(s) | 530, 1409 |
| BstAUI | TGTACA | 1 cut(s) | 1593 |
| BstBI | TTCGAA | 1 cut(s) | 794 |
| BstC8I | GCNNGC | 4 cut(s) | 104, 288, 881, 1254 |
| BstDSI | CCRYGG | 1 cut(s) | 597 |
| BstF5I | GGATG | 6 cut(s) | 150, 159, 448, 1332, 1540, 1923 |
| BstFNI | CGCG | 2 cut(s) | 102, 1078 |
| BstHHI | GCGC | 2 cut(s) | 978, 1630 |
| BstKTI | GATC | 9 cut(s) | 42, 63, 127, 159, 312, 394, 931, 1060, 1888 |
| BstMAI | GTCTC | 6 cut(s) | 11, 138, 414, 1793, 1844, 1937 |
| BstMBI | GATC | 9 cut(s) | 39, 60, 124, 156, 309, 391, 928, 1057, 1885 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 32, 287, 535, 638, 769, 778, 973, 1084, 1253 |
| BstNI | CCWGG | 1 cut(s) | 32 |
| BstNSI | RCATGY | 2 cut(s) | 1732, 1984 |
| BstSCI | CCNGG | 3 cut(s) | 30, 257, 290 |
| BstSFI | CTRYAG | 2 cut(s) | 76, 1770 |
| BstUI | CGCG | 2 cut(s) | 102, 1078 |
| BstV1I | GCAGC | 3 cut(s) | 1074, 1824, 1827 |
| BstV2I | GAAGAC | 4 cut(s) | 504, 849, 1607, 1748 |
| BstZ17I | GTATAC | 1 cut(s) | 1179 |
| BsuI | GTATCC | 1 cut(s) | 1232 |
| BsuRI | GGCC | 3 cut(s) | 290, 879, 1338 |
| BtgI | CCRYGG | 1 cut(s) | 597 |
| BtrI | CACGTC | 1 cut(s) | 301 |
| BtsCI | GGATG | 6 cut(s) | 150, 159, 448, 1332, 1540, 1923 |
| BtsIMutI | CAGTG | 1 cut(s) | 1287 |
| Cac8I | GCNNGC | 4 cut(s) | 104, 288, 881, 1254 |
| CaiI | CAGNNNCTG | 1 cut(s) | 52 |
| CfoI | GCGC | 2 cut(s) | 978, 1630 |
| Cfr10I | RCCGGY | 2 cut(s) | 200, 1361 |
| Cfr13I | GGNCC | 4 cut(s) | 255, 261, 471, 1336 |
| Csp6I | GTAC | 4 cut(s) | 731, 1021, 1270, 1594 |
| CspAI | ACCGGT | 1 cut(s) | 1361 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 383, 418 |
| CviAII | CATG | 8 cut(s) | 211, 307, 734, 1226, 1675, 1717, 1729, 1981 |
| CviQI | GTAC | 4 cut(s) | 731, 1021, 1270, 1594 |
| DpnI | GATC | 9 cut(s) | 41, 62, 126, 158, 311, 393, 930, 1059, 1887 |
| DpnII | GATC | 9 cut(s) | 39, 60, 124, 156, 309, 391, 928, 1057, 1885 |
| Eam1104I | CTCTTC | 3 cut(s) | 908, 1539, 1784 |
| EarI | CTCTTC | 3 cut(s) | 908, 1539, 1784 |
| EciI | GGCGGA | 1 cut(s) | 50 |
| Ecl136II | GAGCTC | 1 cut(s) | 106 |
| Eco130I | CCWWGG | 3 cut(s) | 474, 1024, 1605 |
| Eco24I | GRGCYC | 1 cut(s) | 108 |
| Eco31I | GGTCTC | 3 cut(s) | 11, 1844, 1937 |
| Eco47I | GGWCC | 3 cut(s) | 255, 261, 471 |
| Eco53kI | GAGCTC | 1 cut(s) | 106 |
| Eco57I | CTGAAG | 1 cut(s) | 1508 |
| Eco88I | CYCGRG | 3 cut(s) | 128, 884, 1142 |
| EcoICRI | GAGCTC | 1 cut(s) | 106 |
| EcoRII | CCWGG | 1 cut(s) | 30 |
| EcoT14I | CCWWGG | 3 cut(s) | 474, 1024, 1605 |
| EcoT22I | ATGCAT | 1 cut(s) | 152 |
| EcoT38I | GRGCYC | 1 cut(s) | 108 |
| ErhI | CCWWGG | 3 cut(s) | 474, 1024, 1605 |
| FaeI | CATG | 8 cut(s) | 214, 310, 737, 1229, 1678, 1720, 1732, 1984 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 834, 866, 907, 939, 1506, 1538 |
| FaqI | GGGAC | 4 cut(s) | 256, 274, 484, 1721 |
| FatI | CATG | 8 cut(s) | 210, 306, 733, 1225, 1674, 1716, 1728, 1980 |
| FbaI | TGATCA | 2 cut(s) | 60, 391 |
| FblI | GTMKAC | 2 cut(s) | 1178, 1977 |
| Fnu4HI | GCNGC | 4 cut(s) | 1064, 1088, 1838, 1841 |
| FokI | GGATG | 6 cut(s) | 137, 146, 455, 1339, 1547, 1930 |
| FriOI | GRGCYC | 1 cut(s) | 108 |
| Fsp4HI | GCNGC | 4 cut(s) | 1064, 1088, 1838, 1841 |
| FspBI | CTAG | 3 cut(s) | 438, 1734, 1964 |
| FspI | TGCGCA | 1 cut(s) | 977 |
| GlaI | GCGC | 2 cut(s) | 977, 1629 |
| GluI | GCNGC | 4 cut(s) | 1064, 1088, 1838, 1841 |
| GsaI | CCCAGC | 1 cut(s) | 1905 |
| GsuI | CTGGAG | 1 cut(s) | 1421 |
| HaeIII | GGCC | 3 cut(s) | 290, 879, 1338 |
| HapII | CCGG | 7 cut(s) | 154, 201, 258, 291, 782, 1016, 1362 |
| HhaI | GCGC | 2 cut(s) | 978, 1630 |
| Hin1II | CATG | 8 cut(s) | 214, 310, 737, 1229, 1678, 1720, 1732, 1984 |
| Hin6I | GCGC | 2 cut(s) | 976, 1628 |
| HinP1I | GCGC | 2 cut(s) | 976, 1628 |
| HincII | GTYRAC | 3 cut(s) | 175, 1978, 2032 |
| HindII | GTYRAC | 3 cut(s) | 175, 1978, 2032 |
| HindIII | AAGCTT | 2 cut(s) | 527, 965 |
| HinfI | GANTC | 8 cut(s) | 131, 330, 407, 1049, 1102, 1123, 1538, 1909 |
| HpaII | CCGG | 7 cut(s) | 154, 201, 258, 291, 782, 1016, 1362 |
| HphI | GGTGA | 9 cut(s) | 178, 241, 490, 671, 730, 1123, 1141, 1376, 1834 |
| Hpy166II | GTNNAC | 6 cut(s) | 175, 232, 1179, 1665, 1978, 2032 |
| Hpy188I | TCNGA | 6 cut(s) | 54, 124, 556, 1054, 1543, 1648 |
| Hpy188III | TCNNGA | 9 cut(s) | 128, 154, 1144, 1201, 1321, 1384, 1400, 1760, 1798 |
| Hpy8I | GTNNAC | 6 cut(s) | 175, 232, 1179, 1665, 1978, 2032 |
| HpyCH4III | ACNGT | 3 cut(s) | 433, 598, 1473 |
| HpyCH4IV | ACGT | 2 cut(s) | 12, 300 |
| HpyCH4V | TGCA | 5 cut(s) | 150, 286, 632, 1090, 1163 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 32, 287, 535, 638, 769, 778, 973, 1084, 1253 |
| HpySE526I | ACGT | 2 cut(s) | 12, 300 |
| Hsp92II | CATG | 8 cut(s) | 214, 310, 737, 1229, 1678, 1720, 1732, 1984 |
| HspAI | GCGC | 2 cut(s) | 976, 1628 |
| Kpn2I | TCCGGA | 1 cut(s) | 153 |
| Ksp22I | TGATCA | 2 cut(s) | 60, 391 |
| Kzo9I | GATC | 9 cut(s) | 39, 60, 124, 156, 309, 391, 928, 1057, 1885 |
| LmnI | GCTCC | 5 cut(s) | 535, 742, 1006, 1721, 1780 |
| Lsp1109I | GCAGC | 3 cut(s) | 1074, 1824, 1827 |
| LweI | GCATC | 5 cut(s) | 159, 619, 1002, 1074, 1908 |
| MaeI | CTAG | 3 cut(s) | 438, 1734, 1964 |
| MaeII | ACGT | 2 cut(s) | 12, 300 |
| MaeIII | GTNAC | 4 cut(s) | 370, 433, 1318, 1507 |
| MalI | GATC | 9 cut(s) | 41, 62, 126, 158, 311, 393, 930, 1059, 1887 |
| MbiI | CCGCTC | 2 cut(s) | 1066, 1856 |
| MboI | GATC | 9 cut(s) | 39, 60, 124, 156, 309, 391, 928, 1057, 1885 |
| MfeI | CAATTG | 1 cut(s) | 997 |
| MhlI | GDGCHC | 2 cut(s) | 108, 922 |
| MluCI | AATT | 4 cut(s) | 235, 997, 1184, 1683 |
| MlyI | GAGTC | 3 cut(s) | 140, 416, 1547 |
| MmeI | TCCRAC | 2 cut(s) | 1447, 1521 |
| Mph1103I | ATGCAT | 1 cut(s) | 152 |
| MroI | TCCGGA | 1 cut(s) | 153 |
| MroXI | GAANNNNTTC | 2 cut(s) | 863, 1521 |
| MseI | TTAA | 5 cut(s) | 447, 531, 1308, 1410, 1755 |
| MslI | CAYNNNNRTG | 3 cut(s) | 368, 384, 1325 |
| MspCI | CTTAAG | 2 cut(s) | 530, 1409 |
| MspI | CCGG | 7 cut(s) | 154, 201, 258, 291, 782, 1016, 1362 |
| MspR9I | CCNGG | 3 cut(s) | 32, 259, 292 |
| MunI | CAATTG | 1 cut(s) | 997 |
| Mva1269I | GAATGC | 2 cut(s) | 26, 550 |
| MvaI | CCWGG | 1 cut(s) | 32 |
| MvnI | CGCG | 2 cut(s) | 102, 1078 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 32, 287, 535, 638, 769, 778, 973, 1084, 1253 |
| NciI | CCSGG | 2 cut(s) | 259, 292 |
| NdeII | GATC | 9 cut(s) | 39, 60, 124, 156, 309, 391, 928, 1057, 1885 |
| NlaIII | CATG | 8 cut(s) | 214, 310, 737, 1229, 1678, 1720, 1732, 1984 |
| NlaIV | GGNNCC | 2 cut(s) | 262, 472 |
| NmuCI | GTSAC | 2 cut(s) | 370, 1318 |
| NsbI | TGCGCA | 1 cut(s) | 977 |
| NsiI | ATGCAT | 1 cut(s) | 152 |
| NspI | RCATGY | 2 cut(s) | 1732, 1984 |
| NspV | TTCGAA | 1 cut(s) | 794 |
| PaeR7I | CTCGAG | 3 cut(s) | 128, 884, 1142 |
| PciI | ACATGT | 1 cut(s) | 1728 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 201 |
| PctI | GAATGC | 2 cut(s) | 26, 550 |
| PdmI | GAANNNNTTC | 2 cut(s) | 863, 1521 |
| PfeI | GAWTC | 5 cut(s) | 330, 1049, 1102, 1123, 1909 |
| PflFI | GACNNNGTC | 2 cut(s) | 170, 1316 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 837 |
| PfoI | TCCNGGA | 1 cut(s) | 257 |
| PinAI | ACCGGT | 1 cut(s) | 1361 |
| PkrI | GCNGC | 4 cut(s) | 1065, 1089, 1839, 1842 |
| PleI | GAGTC | 3 cut(s) | 139, 415, 1546 |
| PpsI | GAGTC | 3 cut(s) | 139, 415, 1546 |
| PscI | ACATGT | 1 cut(s) | 1728 |
| PshBI | ATTAAT | 1 cut(s) | 1755 |
| PsiI | TTATAA | 2 cut(s) | 563, 989 |
| Psp124BI | GAGCTC | 1 cut(s) | 108 |
| Psp6I | CCWGG | 1 cut(s) | 30 |
| PspFI | CCCAGC | 1 cut(s) | 1901 |
| PspGI | CCWGG | 1 cut(s) | 30 |
| PspN4I | GGNNCC | 2 cut(s) | 262, 472 |
| PspPI | GGNCC | 4 cut(s) | 255, 261, 471, 1336 |
| PspXI | VCTCGAGB | 1 cut(s) | 884 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1337, 1369 |
| PstNI | CAGNNNCTG | 1 cut(s) | 52 |
| PsyI | GACNNNGTC | 2 cut(s) | 170, 1316 |
| RsaI | GTAC | 4 cut(s) | 732, 1022, 1271, 1595 |
| RsaNI | GTAC | 4 cut(s) | 731, 1021, 1270, 1594 |
| RseI | CAYNNNNRTG | 3 cut(s) | 368, 384, 1325 |
| SacI | GAGCTC | 1 cut(s) | 108 |
| SalI | GTCGAC | 1 cut(s) | 1976 |
| SaqAI | TTAA | 5 cut(s) | 447, 531, 1308, 1410, 1755 |
| SatI | GCNGC | 4 cut(s) | 1064, 1088, 1838, 1841 |
| Sau3AI | GATC | 9 cut(s) | 39, 60, 124, 156, 309, 391, 928, 1057, 1885 |
| Sau96I | GGNCC | 4 cut(s) | 255, 261, 471, 1336 |
| SchI | GAGTC | 3 cut(s) | 140, 416, 1547 |
| ScrFI | CCNGG | 3 cut(s) | 32, 259, 292 |
| SduI | GDGCHC | 2 cut(s) | 108, 922 |
| SfaNI | GCATC | 5 cut(s) | 159, 619, 1002, 1074, 1908 |
| SfcI | CTRYAG | 2 cut(s) | 76, 1770 |
| Sfr274I | CTCGAG | 3 cut(s) | 128, 884, 1142 |
| SfuI | TTCGAA | 1 cut(s) | 794 |
| SgrAI | CRCCGGYG | 1 cut(s) | 200 |
| SinI | GGWCC | 3 cut(s) | 255, 261, 471 |
| SlaI | CTCGAG | 3 cut(s) | 128, 884, 1142 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 368, 384, 1325 |
| SmlI | CTYRAG | 9 cut(s) | 128, 491, 530, 884, 1106, 1142, 1211, 1409, 1529 |
| SmoI | CTYRAG | 9 cut(s) | 128, 491, 530, 884, 1106, 1142, 1211, 1409, 1529 |
| SpeI | ACTAGT | 1 cut(s) | 1963 |
| Sse9I | AATT | 4 cut(s) | 235, 997, 1184, 1683 |
| SsiI | CCGC | 4 cut(s) | 35, 100, 1064, 1854 |
| SspMI | CTAG | 3 cut(s) | 438, 1734, 1964 |
| SstI | GAGCTC | 1 cut(s) | 108 |
| StyD4I | CCNGG | 3 cut(s) | 30, 257, 290 |
| StyI | CCWWGG | 3 cut(s) | 474, 1024, 1605 |
| TaaI | ACNGT | 3 cut(s) | 433, 598, 1473 |
| TaiI | ACGT | 2 cut(s) | 15, 303 |
| TasI | AATT | 4 cut(s) | 235, 997, 1184, 1683 |
| TatI | WGTACW | 1 cut(s) | 1593 |
| TauI | GCSGC | 1 cut(s) | 1066 |
| TfiI | GAWTC | 5 cut(s) | 330, 1049, 1102, 1123, 1909 |
| Tru1I | TTAA | 5 cut(s) | 447, 531, 1308, 1410, 1755 |
| Tru9I | TTAA | 5 cut(s) | 447, 531, 1308, 1410, 1755 |
| TscAI | CASTG | 1 cut(s) | 1294 |
| TseFI | GTSAC | 2 cut(s) | 370, 1318 |
| TseI | GCWGC | 3 cut(s) | 1087, 1837, 1840 |
| Tsp45I | GTSAC | 2 cut(s) | 370, 1318 |
| TspDTI | ATGAA | 4 cut(s) | 636, 1343, 1703, 1959 |
| TspRI | CASTG | 1 cut(s) | 1294 |
| Tth111I | GACNNNGTC | 2 cut(s) | 170, 1316 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 837 |
| Vha464I | CTTAAG | 2 cut(s) | 530, 1409 |
| VpaK11BI | GGWCC | 3 cut(s) | 255, 261, 471 |
| VspI | ATTAAT | 1 cut(s) | 1755 |
| XapI | RAATTY | 1 cut(s) | 1184 |
| XceI | RCATGY | 2 cut(s) | 1732, 1984 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 223, 271 |
| XhoI | CTCGAG | 3 cut(s) | 128, 884, 1142 |
| XmiI | GTMKAC | 2 cut(s) | 1178, 1977 |
| XmnI | GAANNNNTTC | 2 cut(s) | 863, 1521 |
| XspI | CTAG | 3 cut(s) | 438, 1734, 1964 |
| Zsp2I | ATGCAT | 1 cut(s) | 152 |