Heat shock protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 626 |
| AciI | CCGC | 2 cut(s) | 740, 764 |
| AclWI | GGATC | 1 cut(s) | 145 |
| AcuI | CTGAAG | 2 cut(s) | 176, 332 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 572, 701 |
| AflIII | ACRYGT | 1 cut(s) | 534 |
| AgsI | TTSAA | 3 cut(s) | 140, 202, 256 |
| AluBI | AGCT | 9 cut(s) | 16, 58, 286, 316, 589, 608, 662, 700, 754 |
| AluI | AGCT | 9 cut(s) | 16, 58, 286, 316, 589, 608, 662, 700, 754 |
| Alw21I | GWGCWC | 2 cut(s) | 60, 532 |
| Alw26I | GTCTC | 3 cut(s) | 387, 650, 719 |
| AlwI | GGATC | 1 cut(s) | 145 |
| ApeKI | GCWGC | 3 cut(s) | 444, 643, 646 |
| AspS9I | GGNCC | 1 cut(s) | 634 |
| AsuHPI | GGTGA | 3 cut(s) | 172, 429, 470 |
| AvaII | GGWCC | 1 cut(s) | 634 |
| BanII | GRGCYC | 1 cut(s) | 60 |
| BauI | CACGAG | 2 cut(s) | 126, 287 |
| BbsI | GAAGAC | 2 cut(s) | 554, 726 |
| Bbv12I | GWGCWC | 2 cut(s) | 60, 532 |
| BbvCI | CCTCAGC | 2 cut(s) | 590, 768 |
| BbvI | GCAGC | 3 cut(s) | 431, 630, 633 |
| BccI | CCATC | 2 cut(s) | 269, 462 |
| BclI | TGATCA | 1 cut(s) | 162 |
| BcoDI | GTCTC | 3 cut(s) | 387, 650, 719 |
| BisI | GCNGC | 4 cut(s) | 445, 644, 647, 765 |
| BlpI | GCTNAGC | 1 cut(s) | 707 |
| BlsI | GCNGC | 4 cut(s) | 446, 645, 648, 766 |
| Bme18I | GGWCC | 1 cut(s) | 634 |
| BmgT120I | GGNCC | 1 cut(s) | 634 |
| BmsI | GCATC | 4 cut(s) | 123, 585, 727, 751 |
| BpiI | GAAGAC | 2 cut(s) | 554, 726 |
| BpmI | CTGGAG | 1 cut(s) | 776 |
| Bpu10I | CCTNAGC | 4 cut(s) | 501, 590, 750, 768 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 707 |
| BsaJI | CCNNGG | 1 cut(s) | 552 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 384, 414 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 572, 701 |
| Bse1I | ACTGG | 1 cut(s) | 460 |
| BseDI | CCNNGG | 1 cut(s) | 552 |
| BseGI | GGATG | 3 cut(s) | 473, 742, 766 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 572, 701 |
| BseMII | CTCAG | 7 cut(s) | 204, 321, 515, 523, 581, 741, 759 |
| BseNI | ACTGG | 1 cut(s) | 460 |
| BseRI | GAGGAG | 4 cut(s) | 40, 68, 599, 785 |
| BseXI | GCAGC | 3 cut(s) | 431, 630, 633 |
| BseYI | CCCAGC | 1 cut(s) | 700 |
| BsiHKAI | GWGCWC | 2 cut(s) | 60, 532 |
| BslFI | GGGAC | 1 cut(s) | 527 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 572, 701 |
| BsmAI | GTCTC | 3 cut(s) | 387, 650, 719 |
| BsmFI | GGGAC | 1 cut(s) | 527 |
| Bsp1286I | GDGCHC | 2 cut(s) | 60, 532 |
| Bsp143I | GATC | 2 cut(s) | 150, 162 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 707 |
| Bsp19I | CCATGG | 1 cut(s) | 552 |
| BspACI | CCGC | 2 cut(s) | 740, 764 |
| BspCNI | CTCAG | 7 cut(s) | 205, 322, 514, 522, 582, 742, 760 |
| BspPI | GGATC | 1 cut(s) | 145 |
| BsrI | ACTGG | 1 cut(s) | 460 |
| BssECI | CCNNGG | 1 cut(s) | 552 |
| BssMI | GATC | 2 cut(s) | 150, 162 |
| BssSI | CACGAG | 2 cut(s) | 126, 287 |
| BssT1I | CCWWGG | 1 cut(s) | 552 |
| Bst2BI | CACGAG | 2 cut(s) | 126, 287 |
| Bst4CI | ACNGT | 1 cut(s) | 730 |
| Bst6I | CTCTTC | 2 cut(s) | 330, 590 |
| BstDEI | CTNAG | 8 cut(s) | 213, 330, 501, 509, 590, 707, 750, 768 |
| BstDSI | CCRYGG | 1 cut(s) | 552 |
| BstF5I | GGATG | 3 cut(s) | 473, 742, 766 |
| BstKTI | GATC | 2 cut(s) | 153, 165 |
| BstMAI | GTCTC | 3 cut(s) | 387, 650, 719 |
| BstMBI | GATC | 2 cut(s) | 150, 162 |
| BstNSI | RCATGY | 2 cut(s) | 538, 748 |
| BstV1I | GCAGC | 3 cut(s) | 431, 630, 633 |
| BstV2I | GAAGAC | 2 cut(s) | 554, 726 |
| BstX2I | RGATCY | 1 cut(s) | 150 |
| BstYI | RGATCY | 1 cut(s) | 150 |
| BtgI | CCRYGG | 1 cut(s) | 552 |
| BtsCI | GGATG | 3 cut(s) | 473, 742, 766 |
| BtsIMutI | CAGTG | 1 cut(s) | 453 |
| Cfr13I | GGNCC | 1 cut(s) | 634 |
| CviAII | CATG | 6 cut(s) | 481, 523, 535, 553, 580, 745 |
| DdeI | CTNAG | 8 cut(s) | 213, 330, 501, 509, 590, 707, 750, 768 |
| DpnI | GATC | 2 cut(s) | 152, 164 |
| DpnII | GATC | 2 cut(s) | 150, 162 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 626 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 626 |
| Eam1104I | CTCTTC | 2 cut(s) | 330, 590 |
| EarI | CTCTTC | 2 cut(s) | 330, 590 |
| Ecl136II | GAGCTC | 1 cut(s) | 58 |
| Eco130I | CCWWGG | 1 cut(s) | 552 |
| Eco24I | GRGCYC | 1 cut(s) | 60 |
| Eco47I | GGWCC | 1 cut(s) | 634 |
| Eco53kI | GAGCTC | 1 cut(s) | 58 |
| Eco57I | CTGAAG | 2 cut(s) | 176, 332 |
| EcoICRI | GAGCTC | 1 cut(s) | 58 |
| EcoO109I | RGGNCCY | 1 cut(s) | 634 |
| EcoT14I | CCWWGG | 1 cut(s) | 552 |
| EcoT38I | GRGCYC | 1 cut(s) | 60 |
| ErhI | CCWWGG | 1 cut(s) | 552 |
| FaeI | CATG | 6 cut(s) | 484, 526, 538, 556, 583, 748 |
| FaqI | GGGAC | 1 cut(s) | 527 |
| FatI | CATG | 6 cut(s) | 480, 522, 534, 552, 579, 744 |
| FbaI | TGATCA | 1 cut(s) | 162 |
| Fnu4HI | GCNGC | 4 cut(s) | 445, 644, 647, 765 |
| FokI | GGATG | 3 cut(s) | 480, 749, 773 |
| FriOI | GRGCYC | 1 cut(s) | 60 |
| Fsp4HI | GCNGC | 4 cut(s) | 445, 644, 647, 765 |
| GluI | GCNGC | 4 cut(s) | 445, 644, 647, 765 |
| GsaI | CCCAGC | 1 cut(s) | 704 |
| GsuI | CTGGAG | 1 cut(s) | 776 |
| Hin1II | CATG | 6 cut(s) | 484, 526, 538, 556, 583, 748 |
| HincII | GTYRAC | 1 cut(s) | 433 |
| HindII | GTYRAC | 1 cut(s) | 433 |
| HindIII | AAGCTT | 1 cut(s) | 314 |
| HinfI | GANTC | 4 cut(s) | 98, 245, 361, 678 |
| HphI | GGTGA | 3 cut(s) | 172, 429, 470 |
| Hpy166II | GTNNAC | 3 cut(s) | 97, 433, 793 |
| Hpy188I | TCNGA | 3 cut(s) | 103, 312, 421 |
| Hpy188III | TCNNGA | 5 cut(s) | 65, 128, 154, 166, 675 |
| Hpy8I | GTNNAC | 3 cut(s) | 97, 433, 793 |
| HpyAV | CCTTC | 4 cut(s) | 151, 299, 400, 490 |
| HpyCH4III | ACNGT | 1 cut(s) | 730 |
| HpyCH4V | TGCA | 3 cut(s) | 189, 369, 476 |
| HpyF3I | CTNAG | 8 cut(s) | 213, 330, 501, 509, 590, 707, 750, 768 |
| Hsp92II | CATG | 6 cut(s) | 484, 526, 538, 556, 583, 748 |
| Ksp22I | TGATCA | 1 cut(s) | 162 |
| Kzo9I | GATC | 2 cut(s) | 150, 162 |
| LmnI | GCTCC | 2 cut(s) | 55, 586 |
| Lsp1109I | GCAGC | 3 cut(s) | 431, 630, 633 |
| LweI | GCATC | 4 cut(s) | 123, 585, 727, 751 |
| MaeIII | GTNAC | 1 cut(s) | 451 |
| MalI | GATC | 2 cut(s) | 152, 164 |
| MboI | GATC | 2 cut(s) | 150, 162 |
| MflI | RGATCY | 1 cut(s) | 150 |
| MhlI | GDGCHC | 2 cut(s) | 60, 532 |
| MluCI | AATT | 2 cut(s) | 375, 426 |
| MlyI | GAGTC | 2 cut(s) | 92, 254 |
| MseI | TTAA | 2 cut(s) | 12, 378 |
| MslI | CAYNNNNRTG | 1 cut(s) | 194 |
| NcoI | CCATGG | 1 cut(s) | 552 |
| NdeII | GATC | 2 cut(s) | 150, 162 |
| NlaIII | CATG | 6 cut(s) | 484, 526, 538, 556, 583, 748 |
| NmuCI | GTSAC | 1 cut(s) | 451 |
| NspI | RCATGY | 2 cut(s) | 538, 748 |
| PciI | ACATGT | 1 cut(s) | 534 |
| PfeI | GAWTC | 2 cut(s) | 361, 678 |
| PkrI | GCNGC | 4 cut(s) | 446, 645, 648, 766 |
| PleI | GAGTC | 2 cut(s) | 92, 253 |
| PpsI | GAGTC | 2 cut(s) | 92, 253 |
| PpuMI | RGGWCCY | 1 cut(s) | 634 |
| PscI | ACATGT | 1 cut(s) | 534 |
| Psp124BI | GAGCTC | 1 cut(s) | 60 |
| Psp5II | RGGWCCY | 1 cut(s) | 634 |
| PspFI | CCCAGC | 1 cut(s) | 700 |
| PspPI | GGNCC | 1 cut(s) | 634 |
| PspPPI | RGGWCCY | 1 cut(s) | 634 |
| PsuI | RGATCY | 1 cut(s) | 150 |
| RseI | CAYNNNNRTG | 1 cut(s) | 194 |
| SacI | GAGCTC | 1 cut(s) | 60 |
| SaqAI | TTAA | 2 cut(s) | 12, 378 |
| SatI | GCNGC | 4 cut(s) | 445, 644, 647, 765 |
| Sau3AI | GATC | 2 cut(s) | 150, 162 |
| Sau96I | GGNCC | 1 cut(s) | 634 |
| SchI | GAGTC | 2 cut(s) | 92, 254 |
| SduI | GDGCHC | 2 cut(s) | 60, 532 |
| SfaNI | GCATC | 4 cut(s) | 123, 585, 727, 751 |
| SinI | GGWCC | 1 cut(s) | 634 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 194 |
| Sse9I | AATT | 2 cut(s) | 375, 426 |
| SsiI | CCGC | 2 cut(s) | 740, 764 |
| SstI | GAGCTC | 1 cut(s) | 60 |
| StyI | CCWWGG | 1 cut(s) | 552 |
| TaaI | ACNGT | 1 cut(s) | 730 |
| TasI | AATT | 2 cut(s) | 375, 426 |
| TauI | GCSGC | 1 cut(s) | 767 |
| TfiI | GAWTC | 2 cut(s) | 361, 678 |
| Tru1I | TTAA | 2 cut(s) | 12, 378 |
| Tru9I | TTAA | 2 cut(s) | 12, 378 |
| TscAI | CASTG | 1 cut(s) | 460 |
| TseFI | GTSAC | 1 cut(s) | 451 |
| TseI | GCWGC | 3 cut(s) | 444, 643, 646 |
| Tsp45I | GTSAC | 1 cut(s) | 451 |
| TspDTI | ATGAA | 5 cut(s) | 17, 194, 339, 509, 732 |
| TspGWI | ACGGA | 1 cut(s) | 616 |
| TspRI | CASTG | 1 cut(s) | 460 |
| VpaK11BI | GGWCC | 1 cut(s) | 634 |
| XceI | RCATGY | 2 cut(s) | 538, 748 |