Heat shock protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 623, 1049 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1715 |
| Acc16I | TGCGCA | 1 cut(s) | 1037 |
| Acc65I | GGTACC | 1 cut(s) | 1329 |
| AccB1I | GGYRCC | 1 cut(s) | 1329 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 897 |
| AccBSI | CCGCTC | 2 cut(s) | 1126, 1949 |
| AccI | GTMKAC | 3 cut(s) | 1238, 1719, 2070 |
| AccII | CGCG | 2 cut(s) | 117, 1138 |
| AccIII | TCCGGA | 1 cut(s) | 213 |
| AciI | CCGC | 4 cut(s) | 50, 115, 1124, 1947 |
| AclWI | GGATC | 4 cut(s) | 148, 170, 224, 1973 |
| AcsI | RAATTY | 1 cut(s) | 1244 |
| AcuI | CTGAAG | 1 cut(s) | 1568 |
| AdeI | CACNNNGTG | 1 cut(s) | 1730 |
| AfaI | GTAC | 3 cut(s) | 792, 1082, 1331 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 229, 897, 1276, 1303 |
| AflII | CTTAAG | 1 cut(s) | 1469 |
| AflIII | ACRYGT | 1 cut(s) | 1821 |
| AgsI | TTSAA | 4 cut(s) | 8, 952, 1213, 1537 |
| AhlI | ACTAGT | 1 cut(s) | 2056 |
| AjiI | CACGTC | 2 cut(s) | 361, 1824 |
| AjnI | CCWGG | 1 cut(s) | 45 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1259, 1291 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 195, 227 |
| Alw21I | GWGCWC | 2 cut(s) | 123, 982 |
| Alw26I | GTCTC | 6 cut(s) | 26, 198, 474, 1886, 1937, 2030 |
| AlwI | GGATC | 4 cut(s) | 148, 170, 224, 1973 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 67 |
| Ama87I | CYCGRG | 3 cut(s) | 188, 944, 1202 |
| Aor13HI | TCCGGA | 1 cut(s) | 213 |
| AoxI | GGCC | 3 cut(s) | 348, 937, 1396 |
| ApeKI | GCWGC | 3 cut(s) | 1147, 1930, 1933 |
| ApoI | RAATTY | 1 cut(s) | 1244 |
| AseI | ATTAAT | 1 cut(s) | 1848 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 923, 1581 |
| Asp718I | GGTACC | 1 cut(s) | 1329 |
| AspLEI | GCGC | 2 cut(s) | 1038, 1690 |
| AspS9I | GGNCC | 3 cut(s) | 315, 531, 1396 |
| AsuC2I | CCSGG | 2 cut(s) | 319, 352 |
| AsuII | TTCGAA | 1 cut(s) | 854 |
| AvaI | CYCGRG | 3 cut(s) | 188, 944, 1202 |
| AvaII | GGWCC | 2 cut(s) | 315, 531 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 782, 815 |
| BanI | GGYRCC | 1 cut(s) | 1329 |
| BanII | GRGCYC | 1 cut(s) | 123 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1394, 1426 |
| BauI | CACGAG | 2 cut(s) | 1000, 1381 |
| BbsI | GAAGAC | 6 cut(s) | 244, 564, 659, 909, 1667, 1841 |
| Bbv12I | GWGCWC | 2 cut(s) | 123, 982 |
| BbvCI | CCTCAGC | 2 cut(s) | 833, 1877 |
| BbvI | GCAGC | 3 cut(s) | 1134, 1917, 1920 |
| BccI | CCATC | 5 cut(s) | 10, 775, 1541, 2089, 2107 |
| BceAI | ACGGC | 1 cut(s) | 1438 |
| BciT130I | CCWGG | 1 cut(s) | 47 |
| BciVI | GTATCC | 1 cut(s) | 1292 |
| BclI | TGATCA | 2 cut(s) | 75, 451 |
| BcnI | CCSGG | 2 cut(s) | 319, 352 |
| BcoDI | GTCTC | 6 cut(s) | 26, 198, 474, 1886, 1937, 2030 |
| BcuI | ACTAGT | 1 cut(s) | 2056 |
| BfaI | CTAG | 2 cut(s) | 1827, 2057 |
| BfmI | CTRYAG | 2 cut(s) | 91, 1863 |
| BfrI | CTTAAG | 1 cut(s) | 1469 |
| BfuI | GTATCC | 1 cut(s) | 1292 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 829 |
| BisI | GCNGC | 4 cut(s) | 1124, 1148, 1931, 1934 |
| BlsI | GCNGC | 4 cut(s) | 1125, 1149, 1932, 1935 |
| Bme1390I | CCNGG | 3 cut(s) | 47, 319, 352 |
| Bme18I | GGWCC | 2 cut(s) | 315, 531 |
| BmeT110I | CYCGRG | 3 cut(s) | 188, 944, 1202 |
| BmgBI | CACGTC | 2 cut(s) | 361, 1824 |
| BmgT120I | GGNCC | 3 cut(s) | 315, 531, 1396 |
| BmiI | GGNNCC | 2 cut(s) | 532, 1331 |
| BmrFI | CCNGG | 3 cut(s) | 47, 319, 352 |
| BmrI | ACTGGG | 1 cut(s) | 2030 |
| BmsI | GCATC | 6 cut(s) | 130, 219, 679, 1062, 1134, 2001 |
| BmuI | ACTGGG | 1 cut(s) | 2030 |
| BpiI | GAAGAC | 6 cut(s) | 244, 564, 659, 909, 1667, 1841 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 854, 886, 1244, 1276 |
| BpmI | CTGGAG | 2 cut(s) | 1481, 1764 |
| Bpu10I | CCTNAGC | 4 cut(s) | 833, 1325, 1788, 1877 |
| Bpu14I | TTCGAA | 1 cut(s) | 854 |
| BpuEI | CTTGAG | 5 cut(s) | 536, 575, 1151, 1256, 1610 |
| BpuMI | CCSGG | 2 cut(s) | 319, 352 |
| BsaBI | GATNNNNATC | 1 cut(s) | 450 |
| BsaI | GGTCTC | 3 cut(s) | 26, 1937, 2030 |
| BsaJI | CCNNGG | 5 cut(s) | 351, 534, 657, 1084, 1665 |
| BsaWI | WCCGGW | 1 cut(s) | 213 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 229, 897, 1276, 1303 |
| Bse118I | RCCGGY | 1 cut(s) | 260 |
| Bse1I | ACTGG | 3 cut(s) | 1426, 1747, 2025 |
| Bse3DI | GCAATG | 1 cut(s) | 136 |
| Bse8I | GATNNNNATC | 1 cut(s) | 450 |
| BseAI | TCCGGA | 1 cut(s) | 213 |
| BseBI | CCWGG | 1 cut(s) | 47 |
| BseDI | CCNNGG | 5 cut(s) | 351, 534, 657, 1084, 1665 |
| BseGI | GGATG | 6 cut(s) | 210, 219, 508, 1392, 1600, 2016 |
| BseJI | GATNNNNATC | 1 cut(s) | 450 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 229, 897, 1276, 1303 |
| BseMI | GCAATG | 1 cut(s) | 136 |
| BseMII | CTCAG | 8 cut(s) | 59, 405, 485, 824, 1339, 1802, 1868, 2041 |
| BseNI | ACTGG | 3 cut(s) | 1426, 1747, 2025 |
| BseRI | GAGGAG | 8 cut(s) | 728, 860, 875, 1079, 1622, 1640, 1886, 2102 |
| BseXI | GCAGC | 3 cut(s) | 1134, 1917, 1920 |
| BseYI | CCCAGC | 2 cut(s) | 179, 1994 |
| Bsh1236I | CGCG | 2 cut(s) | 117, 1138 |
| BshFI | GGCC | 3 cut(s) | 350, 939, 1398 |
| BshNI | GGYRCC | 1 cut(s) | 1329 |
| BsiHKAI | GWGCWC | 2 cut(s) | 123, 982 |
| BsiHKCI | CYCGRG | 3 cut(s) | 188, 944, 1202 |
| BsiSI | CCGG | 6 cut(s) | 214, 261, 318, 351, 842, 1076 |
| BslFI | GGGAC | 4 cut(s) | 316, 334, 544, 1814 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 229, 897, 1276, 1303 |
| BsmAI | GTCTC | 6 cut(s) | 26, 198, 474, 1886, 1937, 2030 |
| BsmFI | GGGAC | 4 cut(s) | 316, 334, 544, 1814 |
| BsmI | GAATGC | 2 cut(s) | 41, 610 |
| BsnI | GGCC | 3 cut(s) | 350, 939, 1398 |
| Bso31I | GGTCTC | 3 cut(s) | 26, 1937, 2030 |
| BsoBI | CYCGRG | 3 cut(s) | 188, 944, 1202 |
| Bsp119I | TTCGAA | 1 cut(s) | 854 |
| Bsp1286I | GDGCHC | 2 cut(s) | 123, 982 |
| Bsp13I | TCCGGA | 1 cut(s) | 213 |
| BspACI | CCGC | 4 cut(s) | 50, 115, 1124, 1947 |
| BspANI | GGCC | 3 cut(s) | 350, 939, 1398 |
| BspCNI | CTCAG | 8 cut(s) | 60, 406, 484, 825, 1338, 1801, 1869, 2040 |
| BspEI | TCCGGA | 1 cut(s) | 213 |
| BspFNI | CGCG | 2 cut(s) | 117, 1138 |
| BspLI | GGNNCC | 2 cut(s) | 532, 1331 |
| BspPI | GGATC | 4 cut(s) | 148, 170, 224, 1973 |
| BspT104I | TTCGAA | 1 cut(s) | 854 |
| BspT107I | GGYRCC | 1 cut(s) | 1329 |
| BspTI | CTTAAG | 1 cut(s) | 1469 |
| BspTNI | GGTCTC | 3 cut(s) | 26, 1937, 2030 |
| BsrBI | CCGCTC | 2 cut(s) | 1126, 1949 |
| BsrDI | GCAATG | 1 cut(s) | 136 |
| BsrFI | RCCGGY | 1 cut(s) | 260 |
| BsrI | ACTGG | 3 cut(s) | 1426, 1747, 2025 |
| BssAI | RCCGGY | 1 cut(s) | 260 |
| BssECI | CCNNGG | 5 cut(s) | 351, 534, 657, 1084, 1665 |
| BssNAI | GTATAC | 1 cut(s) | 1239 |
| BssSI | CACGAG | 2 cut(s) | 1000, 1381 |
| BssT1I | CCWWGG | 3 cut(s) | 534, 1084, 1665 |
| Bst1107I | GTATAC | 1 cut(s) | 1239 |
| Bst2BI | CACGAG | 2 cut(s) | 1000, 1381 |
| Bst2UI | CCWGG | 1 cut(s) | 47 |
| Bst4CI | ACNGT | 4 cut(s) | 250, 493, 658, 1533 |
| Bst6I | CTCTTC | 3 cut(s) | 968, 1599, 1877 |
| BstAFI | CTTAAG | 1 cut(s) | 1469 |
| BstBI | TTCGAA | 1 cut(s) | 854 |
| BstC8I | GCNNGC | 5 cut(s) | 119, 185, 348, 941, 1314 |
| BstDEI | CTNAG | 9 cut(s) | 68, 414, 435, 471, 833, 1325, 1788, 1877, 2027 |
| BstDSI | CCRYGG | 1 cut(s) | 657 |
| BstF5I | GGATG | 6 cut(s) | 210, 219, 508, 1392, 1600, 2016 |
| BstFNI | CGCG | 2 cut(s) | 117, 1138 |
| BstHHI | GCGC | 2 cut(s) | 1038, 1690 |
| BstMAI | GTCTC | 6 cut(s) | 26, 198, 474, 1886, 1937, 2030 |
| BstNI | CCWGG | 1 cut(s) | 47 |
| BstNSI | RCATGY | 1 cut(s) | 2077 |
| BstSCI | CCNGG | 3 cut(s) | 45, 317, 350 |
| BstSFI | CTRYAG | 2 cut(s) | 91, 1863 |
| BstUI | CGCG | 2 cut(s) | 117, 1138 |
| BstV1I | GCAGC | 3 cut(s) | 1134, 1917, 1920 |
| BstV2I | GAAGAC | 6 cut(s) | 244, 564, 659, 909, 1667, 1841 |
| BstX2I | RGATCY | 1 cut(s) | 153 |
| BstYI | RGATCY | 1 cut(s) | 153 |
| BstZ17I | GTATAC | 1 cut(s) | 1239 |
| BsuI | GTATCC | 1 cut(s) | 1292 |
| BsuRI | GGCC | 3 cut(s) | 350, 939, 1398 |
| BtgI | CCRYGG | 1 cut(s) | 657 |
| BtrI | CACGTC | 2 cut(s) | 361, 1824 |
| BtsCI | GGATG | 6 cut(s) | 210, 219, 508, 1392, 1600, 2016 |
| BtsIMutI | CAGTG | 2 cut(s) | 1347, 1740 |
| Cac8I | GCNNGC | 5 cut(s) | 119, 185, 348, 941, 1314 |
| CaiI | CAGNNNCTG | 1 cut(s) | 67 |
| CfoI | GCGC | 2 cut(s) | 1038, 1690 |
| Cfr10I | RCCGGY | 1 cut(s) | 260 |
| Cfr13I | GGNCC | 3 cut(s) | 315, 531, 1396 |
| Csp6I | GTAC | 3 cut(s) | 791, 1081, 1330 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 443, 478 |
| CviAII | CATG | 7 cut(s) | 271, 367, 794, 1286, 1768, 1810, 2074 |
| CviQI | GTAC | 3 cut(s) | 791, 1081, 1330 |
| DdeI | CTNAG | 9 cut(s) | 68, 414, 435, 471, 833, 1325, 1788, 1877, 2027 |
| DraIII | CACNNNGTG | 1 cut(s) | 1730 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1715 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1715 |
| Eam1104I | CTCTTC | 3 cut(s) | 968, 1599, 1877 |
| EarI | CTCTTC | 3 cut(s) | 968, 1599, 1877 |
| EciI | GGCGGA | 1 cut(s) | 65 |
| Ecl136II | GAGCTC | 1 cut(s) | 121 |
| Eco130I | CCWWGG | 3 cut(s) | 534, 1084, 1665 |
| Eco24I | GRGCYC | 1 cut(s) | 123 |
| Eco31I | GGTCTC | 3 cut(s) | 26, 1937, 2030 |
| Eco47I | GGWCC | 2 cut(s) | 315, 531 |
| Eco53kI | GAGCTC | 1 cut(s) | 121 |
| Eco57I | CTGAAG | 1 cut(s) | 1568 |
| Eco88I | CYCGRG | 3 cut(s) | 188, 944, 1202 |
| EcoICRI | GAGCTC | 1 cut(s) | 121 |
| EcoRII | CCWGG | 1 cut(s) | 45 |
| EcoT14I | CCWWGG | 3 cut(s) | 534, 1084, 1665 |
| EcoT22I | ATGCAT | 2 cut(s) | 145, 212 |
| EcoT38I | GRGCYC | 1 cut(s) | 123 |
| ErhI | CCWWGG | 3 cut(s) | 534, 1084, 1665 |
| FaeI | CATG | 7 cut(s) | 274, 370, 797, 1289, 1771, 1813, 2077 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 894, 926, 967, 999, 1566, 1598 |
| FaqI | GGGAC | 4 cut(s) | 316, 334, 544, 1814 |
| FatI | CATG | 7 cut(s) | 270, 366, 793, 1285, 1767, 1809, 2073 |
| FbaI | TGATCA | 2 cut(s) | 75, 451 |
| FblI | GTMKAC | 3 cut(s) | 1238, 1719, 2070 |
| Fnu4HI | GCNGC | 4 cut(s) | 1124, 1148, 1931, 1934 |
| FokI | GGATG | 6 cut(s) | 197, 206, 515, 1399, 1607, 2023 |
| FriOI | GRGCYC | 1 cut(s) | 123 |
| Fsp4HI | GCNGC | 4 cut(s) | 1124, 1148, 1931, 1934 |
| FspBI | CTAG | 2 cut(s) | 1827, 2057 |
| FspI | TGCGCA | 1 cut(s) | 1037 |
| GlaI | GCGC | 2 cut(s) | 1037, 1689 |
| GluI | GCNGC | 4 cut(s) | 1124, 1148, 1931, 1934 |
| GsaI | CCCAGC | 2 cut(s) | 183, 1998 |
| GsuI | CTGGAG | 2 cut(s) | 1481, 1764 |
| HaeIII | GGCC | 3 cut(s) | 350, 939, 1398 |
| HapII | CCGG | 6 cut(s) | 214, 261, 318, 351, 842, 1076 |
| HhaI | GCGC | 2 cut(s) | 1038, 1690 |
| Hin1II | CATG | 7 cut(s) | 274, 370, 797, 1289, 1771, 1813, 2077 |
| Hin6I | GCGC | 2 cut(s) | 1036, 1688 |
| HinP1I | GCGC | 2 cut(s) | 1036, 1688 |
| HincII | GTYRAC | 4 cut(s) | 235, 1720, 2071, 2125 |
| HindII | GTYRAC | 4 cut(s) | 235, 1720, 2071, 2125 |
| HindIII | AAGCTT | 2 cut(s) | 1025, 1556 |
| HpaII | CCGG | 6 cut(s) | 214, 261, 318, 351, 842, 1076 |
| Hpy166II | GTNNAC | 7 cut(s) | 235, 292, 1239, 1720, 1758, 2071, 2125 |
| Hpy188I | TCNGA | 8 cut(s) | 69, 139, 158, 616, 1114, 1603, 1708, 2086 |
| Hpy188III | TCNNGA | 9 cut(s) | 163, 214, 1204, 1261, 1381, 1444, 1460, 1853, 1891 |
| Hpy8I | GTNNAC | 7 cut(s) | 235, 292, 1239, 1720, 1758, 2071, 2125 |
| HpyCH4III | ACNGT | 4 cut(s) | 250, 493, 658, 1533 |
| HpyCH4IV | ACGT | 3 cut(s) | 27, 360, 1823 |
| HpyCH4V | TGCA | 7 cut(s) | 143, 210, 346, 692, 1150, 1223, 1656 |
| HpyF3I | CTNAG | 9 cut(s) | 68, 414, 435, 471, 833, 1325, 1788, 1877, 2027 |
| HpySE526I | ACGT | 3 cut(s) | 27, 360, 1823 |
| Hsp92II | CATG | 7 cut(s) | 274, 370, 797, 1289, 1771, 1813, 2077 |
| HspAI | GCGC | 2 cut(s) | 1036, 1688 |
| Kpn2I | TCCGGA | 1 cut(s) | 213 |
| KpnI | GGTACC | 1 cut(s) | 1333 |
| Ksp22I | TGATCA | 2 cut(s) | 75, 451 |
| LmnI | GCTCC | 5 cut(s) | 595, 802, 1066, 1814, 1873 |
| Lsp1109I | GCAGC | 3 cut(s) | 1134, 1917, 1920 |
| LweI | GCATC | 6 cut(s) | 130, 219, 679, 1062, 1134, 2001 |
| MaeI | CTAG | 2 cut(s) | 1827, 2057 |
| MaeII | ACGT | 3 cut(s) | 27, 360, 1823 |
| MaeIII | GTNAC | 4 cut(s) | 430, 1378, 1567, 1738 |
| MbiI | CCGCTC | 2 cut(s) | 1126, 1949 |
| MfeI | CAATTG | 1 cut(s) | 1057 |
| MflI | RGATCY | 1 cut(s) | 153 |
| MhlI | GDGCHC | 2 cut(s) | 123, 982 |
| MluCI | AATT | 6 cut(s) | 9, 284, 295, 1057, 1244, 1776 |
| MlyI | GAGTC | 4 cut(s) | 200, 476, 1607, 1715 |
| MmeI | TCCRAC | 2 cut(s) | 1507, 1581 |
| Mph1103I | ATGCAT | 2 cut(s) | 145, 212 |
| MroI | TCCGGA | 1 cut(s) | 213 |
| MroXI | GAANNNNTTC | 2 cut(s) | 923, 1581 |
| MseI | TTAA | 5 cut(s) | 495, 507, 1368, 1470, 1848 |
| MslI | CAYNNNNRTG | 3 cut(s) | 428, 444, 1385 |
| MspCI | CTTAAG | 1 cut(s) | 1469 |
| MspI | CCGG | 6 cut(s) | 214, 261, 318, 351, 842, 1076 |
| MspR9I | CCNGG | 3 cut(s) | 47, 319, 352 |
| MunI | CAATTG | 1 cut(s) | 1057 |
| Mva1269I | GAATGC | 2 cut(s) | 41, 610 |
| MvaI | CCWGG | 1 cut(s) | 47 |
| MvnI | CGCG | 2 cut(s) | 117, 1138 |
| NciI | CCSGG | 2 cut(s) | 319, 352 |
| NlaIII | CATG | 7 cut(s) | 274, 370, 797, 1289, 1771, 1813, 2077 |
| NlaIV | GGNNCC | 2 cut(s) | 532, 1331 |
| NmuCI | GTSAC | 3 cut(s) | 430, 1378, 1738 |
| NsbI | TGCGCA | 1 cut(s) | 1037 |
| NsiI | ATGCAT | 2 cut(s) | 145, 212 |
| NspI | RCATGY | 1 cut(s) | 2077 |
| NspV | TTCGAA | 1 cut(s) | 854 |
| PaeR7I | CTCGAG | 3 cut(s) | 188, 944, 1202 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 261 |
| PctI | GAATGC | 2 cut(s) | 41, 610 |
| PdmI | GAANNNNTTC | 2 cut(s) | 923, 1581 |
| PfeI | GAWTC | 6 cut(s) | 159, 390, 1109, 1162, 1183, 2002 |
| PflFI | GACNNNGTC | 2 cut(s) | 230, 1376 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 897 |
| PfoI | TCCNGGA | 1 cut(s) | 317 |
| PkrI | GCNGC | 4 cut(s) | 1125, 1149, 1932, 1935 |
| PleI | GAGTC | 4 cut(s) | 199, 475, 1606, 1715 |
| PpsI | GAGTC | 4 cut(s) | 199, 475, 1606, 1715 |
| PshBI | ATTAAT | 1 cut(s) | 1848 |
| PsiI | TTATAA | 2 cut(s) | 623, 1049 |
| Psp124BI | GAGCTC | 1 cut(s) | 123 |
| Psp6I | CCWGG | 1 cut(s) | 45 |
| PspFI | CCCAGC | 2 cut(s) | 179, 1994 |
| PspGI | CCWGG | 1 cut(s) | 45 |
| PspN4I | GGNNCC | 2 cut(s) | 532, 1331 |
| PspPI | GGNCC | 3 cut(s) | 315, 531, 1396 |
| PspXI | VCTCGAGB | 2 cut(s) | 188, 944 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1397, 1429 |
| PstNI | CAGNNNCTG | 1 cut(s) | 67 |
| PsuI | RGATCY | 1 cut(s) | 153 |
| PsyI | GACNNNGTC | 2 cut(s) | 230, 1376 |
| RsaI | GTAC | 3 cut(s) | 792, 1082, 1331 |
| RsaNI | GTAC | 3 cut(s) | 791, 1081, 1330 |
| RseI | CAYNNNNRTG | 3 cut(s) | 428, 444, 1385 |
| SacI | GAGCTC | 1 cut(s) | 123 |
| SalI | GTCGAC | 2 cut(s) | 1718, 2069 |
| SaqAI | TTAA | 5 cut(s) | 495, 507, 1368, 1470, 1848 |
| SatI | GCNGC | 4 cut(s) | 1124, 1148, 1931, 1934 |
| Sau96I | GGNCC | 3 cut(s) | 315, 531, 1396 |
| SchI | GAGTC | 4 cut(s) | 200, 476, 1607, 1715 |
| ScrFI | CCNGG | 3 cut(s) | 47, 319, 352 |
| SduI | GDGCHC | 2 cut(s) | 123, 982 |
| SfaNI | GCATC | 6 cut(s) | 130, 219, 679, 1062, 1134, 2001 |
| SfcI | CTRYAG | 2 cut(s) | 91, 1863 |
| Sfr274I | CTCGAG | 3 cut(s) | 188, 944, 1202 |
| SfuI | TTCGAA | 1 cut(s) | 854 |
| SgrAI | CRCCGGYG | 1 cut(s) | 260 |
| SinI | GGWCC | 2 cut(s) | 315, 531 |
| SlaI | CTCGAG | 3 cut(s) | 188, 944, 1202 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 428, 444, 1385 |
| SmlI | CTYRAG | 9 cut(s) | 188, 551, 590, 944, 1166, 1202, 1271, 1469, 1589 |
| SmoI | CTYRAG | 9 cut(s) | 188, 551, 590, 944, 1166, 1202, 1271, 1469, 1589 |
| SpeI | ACTAGT | 1 cut(s) | 2056 |
| Sse9I | AATT | 6 cut(s) | 9, 284, 295, 1057, 1244, 1776 |
| SsiI | CCGC | 4 cut(s) | 50, 115, 1124, 1947 |
| SspMI | CTAG | 2 cut(s) | 1827, 2057 |
| SstI | GAGCTC | 1 cut(s) | 123 |
| StyD4I | CCNGG | 3 cut(s) | 45, 317, 350 |
| StyI | CCWWGG | 3 cut(s) | 534, 1084, 1665 |
| TaaI | ACNGT | 4 cut(s) | 250, 493, 658, 1533 |
| TaiI | ACGT | 3 cut(s) | 30, 363, 1826 |
| TasI | AATT | 6 cut(s) | 9, 284, 295, 1057, 1244, 1776 |
| TauI | GCSGC | 1 cut(s) | 1126 |
| TfiI | GAWTC | 6 cut(s) | 159, 390, 1109, 1162, 1183, 2002 |
| Tru1I | TTAA | 5 cut(s) | 495, 507, 1368, 1470, 1848 |
| Tru9I | TTAA | 5 cut(s) | 495, 507, 1368, 1470, 1848 |
| TscAI | CASTG | 2 cut(s) | 1354, 1747 |
| TseFI | GTSAC | 3 cut(s) | 430, 1378, 1738 |
| TseI | GCWGC | 3 cut(s) | 1147, 1930, 1933 |
| Tsp45I | GTSAC | 3 cut(s) | 430, 1378, 1738 |
| TspDTI | ATGAA | 4 cut(s) | 696, 1403, 1796, 2052 |
| TspRI | CASTG | 2 cut(s) | 1354, 1747 |
| Tth111I | GACNNNGTC | 2 cut(s) | 230, 1376 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 897 |
| Vha464I | CTTAAG | 1 cut(s) | 1469 |
| VpaK11BI | GGWCC | 2 cut(s) | 315, 531 |
| VspI | ATTAAT | 1 cut(s) | 1848 |
| XapI | RAATTY | 1 cut(s) | 1244 |
| XceI | RCATGY | 1 cut(s) | 2077 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 283 |
| XhoI | CTCGAG | 3 cut(s) | 188, 944, 1202 |
| XmiI | GTMKAC | 3 cut(s) | 1238, 1719, 2070 |
| XmnI | GAANNNNTTC | 2 cut(s) | 923, 1581 |
| XspI | CTAG | 2 cut(s) | 1827, 2057 |
| Zsp2I | ATGCAT | 2 cut(s) | 145, 212 |