Rh7BG162200

Heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000335
Physical Location & Seq
Reverse (-)
12533338 .. 12535549
2212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG162200.1

Sequence Viewer

Length: 1809 bp
ATGGTTGAAATTGAGATGGAAGCTGACGTTGAGACCTTCGCATTCCAGGCGGAGATCAACCAGCTTCTGAGTTTGATCATCAACACTTTCTACAGCAACAAAGAGATTTTCCTCCGCGAGCTTATCAGCAATGCCTCTGATGCATTGGACAAGATCCGATTCGAGAGCTTGACTGATCCCAGCAAGCTCGAGTCTCAACCCGAACTATGCATCCGGATCATCCCAGACAAGGAAGACCAGCTCGATTTCTTGGACGAGAGGAAGCTCAAGGAGCTTGTGAAGAAGCATTCCGAGTTTATAAGCTACCCGATTTACCTCTGGGTGGAGAAGACTGTGGAGAAGGAGATTAGTGATGATGAAGATGATGCAGGGGAAGGCAAGAATACGGAAGAGGGGGATGTTGAGGAAGTTTATGAGGACAAAGACAAGGAAGGGAAGCCGAAGAAGAAGCAGGTGAAGGAGGTGGTACATGAGTGGGAGCTACTCAACAAGCAGAAGCCGATTTGGCTGAGGAAGCCGGAGGAGGTTTCGAAGGAGGAGTATTGCACTTTCTACAAGAGCCTTACCAATGATTGGGAAGACCCTCTTGCTTGGAAGCATTTCACTGTAGAAGGGCAGCTCGAGTTCAAGTCAATCCTCTTCTTGCCGAAAAGAGCACCTTTTGATCTGTTTGACACGAGGAAGAAGCCGAACAACATCAAGCTTTATGTGCGCAGGGTGTTTATAATGGACAATTGTGAGGAGCTAATGCCGGAGTACCTTGGATTTGTGAAGGGCGTTGTGGATTCTGATGATCTGCCGCTCAACATTTCGCGTGAGATGCTGCAACAGAACAAGATTCTCAAGGTGATTAGGAAGAATCTGGTGAAGAAGTGCCTCGAGATGTTCAATGAGGTTGCAGAGAATAAGGAGGAGTATACTAAATTCTATGAGGCTTTCTCGAAGAACCTCAAGTTGGGTATCCATGAGGACAGCCAGAACAGGGCGAAGCTGGCTGATTTGCTCAGGTACTACTCCACCAAAAGCACTGATGAGATGACCAGCTTTAAGGACTATGTCACTAGGATGAAGGAGGGCCAAAAGAACATCTACTACATTACTGGTGAGAGCAAGAAGGCTGTCGAGAACTCGCCGTTTCTGGAGAGGCTTAAGAAGAAGGGATATGAGGTGTTGTTTATGGTTGAGCCGATTGATGAGTATGCTGTTGGACAGTTGAAGGAGTATGATGGGAAGAAGCTTGTATCTGTAACGAAAGAAGGGCTGCAGCTGGAGGATGAGTCGGAGGAGGAGAAGAAGCAGAGAGAGGAGAAGAAGAAGTCGTTTGAGAATCTGTGCAAGAAGACCAAGGAGATTCTTGGGGATAGGGTGGAGAAGGTGGTGGTCTCTGACAGAGTTGTGGACTCACCGTGTGTCTTGGTGACTGGAGAGTATGGGTGGACAGCTAACATGGAGAGGATCATGAAAGCTCAGGCTTTGAGGGACAACAGCATGGGAGCTTACATGTCTAGCAAGAAGACTATGGAGATTAATCCCGACAATCCTATAGTGGAGGAGCTGAGGAAGAGGGTCGAGACTGATGAGAATGACAAGTCGGTGAAGGACCTTGTGCTGCTGCTCTATGAGACCGCCCTGTTGAGTTCGGGGTTCAGCCTTGATGATCCCAACAGGTTTGCTGGGAGGATTCACAGGATGCTGAAACTGGGTCTCAGCATTGATGAAGATGATGACCCTGAACTAGTTGATGATGTCGACATGCCTCCATCGGAGGAGAATAAGGTTGAGGAAAGCCAGATGGAAGAAGTTGACTGA

Protein Analysis

602

Amino Acids

70.4

Weight (kDa)

4.85

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP90 PF00183 82 - 594 2.7e-242 Hsp90 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000335)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52640
fragaria_vesca FvH4_2g04730 FvH4_2g04730 FvH4_2g04730 FvH4_2g13180 FvH4_7g30260 FvH4_7g30272
malus_domestica MD00G1081900.v1.1 MD01G1208700.v1.1 MD07G1279100.v1.1 MD07G1279200.v1.1 MD11G1152300.v1.1 MD14G1110500.v1.1 MD14G1110600.v1.1
prunus_persica Prupe.2G301300_v2.0.a1 Prupe.5G041100_v2.0.a1 Prupe.5G105600_v2.0.a1
pyrus_communis pycom01g21930 pycom07g25400 pycom09g11140 pycom14g10530
rosa_chinensis RchiOBHm_Chr1g0327061 RchiOBHm_Chr1g0378391 RchiOBHm_Chr1g0379711 RchiOBHm_Chr3g0488481 RchiOBHm_Chr4g0387881 RchiOBHm_Chr5g0021331 RchiOBHm_Chr5g0021341 RchiOBHm_Chr5g0021351 RchiOBHm_Chr6g0274441 RchiOBHm_Chr7g0196751 RchiOBHm_Chr7g0196771
rosa_laevigata RLG00000003992 RLG00000008392 RLG00000009018 RLG00000013542 RLG00000026364 RLG00000026365 RLG00000026438 RLG00000032605
rosa_multiflora Rmu_co8309643.1_g000001 Rmu_co8423093.1_g000001 Rmu_co8520529.1_g000002 Rmu_sc0000938.1_g000001 Rmu_sc0001083.1_g000031 Rmu_sc0001289.1_g000016 Rmu_sc0001777.1_g000013 Rmu_sc0003392.1_g000006 Rmu_sc0003600.1_g000010 Rmu_sc0004484.1_g000024 Rmu_sc0004847.1_g000017 Rmu_sc0009850.1_g000016 Rmu_sc0013411.1_g000009 Rmu_sc0024337.1_g000001 Rmu_sc0024338.1_g000001 Rmu_sc0029385.1_g000001 Rmu_sc0039495.1_g000001 Rmu_ssc0000259.1_g000042
rosa_roxburghii Rroxscaffold_1G00057180 Rroxscaffold_3G00258970 Rroxscaffold_3G00258980 Rroxscaffold_4G00280480 Rroxscaffold_7G00194220 Rroxscaffold_7G00197660
rosa_rugosa Rorug01G0408500 Rorug05G0066400 Rorug05G0066500 Rorug06G0081900 Rorug07G0036200
rosa_samantha Rh1AG083900 Rh1AG430000 Rh1AG438200 Rh1AG438300 Rh1BG387700 Rh1BG394700 Rh1BG394800 Rh1CG081800 Rh1CG401200 Rh1CG407700 Rh1CG407800 Rh1DG327400 Rh1DG418800 Rh1DG425100 Rh1DG425200 Rh2AG541400 Rh2CG384100 Rh2CG524100 Rh4DG107200 Rh5AG155400 Rh5AG155500 Rh5AG155600 Rh5BG155000 Rh5BG155100 Rh5CG168500 Rh5CG168600 Rh5CG168700 Rh5DG155600 Rh6AG198300 Rh6BG201600 Rh6BG491300 Rh6CG202400 Rh6DG192800 Rh7AG160000 Rh7AG160100 Rh7BG162200 Rh7CG168600 Rh7DG161300
rosa_wichuraiana Rw1G037630 Rw5G013860 Rw6G017240 Rw7G013990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 299, 725
AarI CACCTGC 1 cut(s) 442
Acc16I TGCGCA 1 cut(s) 713
Acc36I ACCTGC 1 cut(s) 442
AccB7I CCANNNNNTGG 1 cut(s) 573
AccBSI CCGCTC 1 cut(s) 802
AccI GTMKAC 2 cut(s) 917, 1749
AccII CGCG 2 cut(s) 117, 814
AccIII TCCGGA 1 cut(s) 213
AciI CCGC 4 cut(s) 50, 115, 800, 1626
AclWI GGATC 5 cut(s) 148, 170, 224, 1463, 1652
AcsI RAATTY 1 cut(s) 923
AdeI CACNNNGTG 1 cut(s) 1409
AfaI GTAC 3 cut(s) 468, 758, 1010
AfiI CCNNNNNNNGG 5 cut(s) 229, 322, 573, 955, 982
AflII CTTAAG 1 cut(s) 1148
AflIII ACRYGT 1 cut(s) 1500
AgsI TTSAA 4 cut(s) 8, 628, 889, 1216
AhlI ACTAGT 1 cut(s) 1735
AjnI CCWGG 1 cut(s) 45
AjuI GAANNNNNNNTTGG 2 cut(s) 938, 970
AloI GAACNNNNNNTCC 2 cut(s) 195, 227
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 6 cut(s) 26, 198, 1387, 1565, 1616, 1709
AlwI GGATC 5 cut(s) 148, 170, 224, 1463, 1652
AlwNI CAGNNNCTG 1 cut(s) 67
Ama87I CYCGRG 3 cut(s) 188, 620, 878
Aor13HI TCCGGA 1 cut(s) 213
AoxI GGCC 1 cut(s) 1075
ApeKI GCWGC 6 cut(s) 616, 823, 1261, 1264, 1609, 1612
ApoI RAATTY 1 cut(s) 923
AseI ATTAAT 1 cut(s) 1527
Asp700I GAANNNNTTC 1 cut(s) 599
AspLEI GCGC 1 cut(s) 714
AspS9I GGNCC 2 cut(s) 1075, 1600
AsuHPI GGTGA 7 cut(s) 466, 859, 877, 1115, 1395, 1429, 1606
AsuII TTCGAA 1 cut(s) 530
AvaI CYCGRG 3 cut(s) 188, 620, 878
AvaII GGWCC 1 cut(s) 1600
BaeI ACNNNNGTAYC 2 cut(s) 458, 491
BauI CACGAG 1 cut(s) 676
BbsI GAAGAC 5 cut(s) 240, 335, 585, 1346, 1520
Bbv12I GWGCWC 1 cut(s) 658
BbvCI CCTCAGC 2 cut(s) 509, 1556
BbvI GCAGC 6 cut(s) 628, 810, 1248, 1276, 1596, 1599
BccI CCATC 4 cut(s) 10, 1220, 1768, 1786
BceAI ACGGC 1 cut(s) 1117
BciT130I CCWGG 1 cut(s) 47
BciVI GTATCC 1 cut(s) 971
BclI TGATCA 1 cut(s) 75
BcoDI GTCTC 6 cut(s) 26, 198, 1387, 1565, 1616, 1709
BcuI ACTAGT 1 cut(s) 1735
BfaI CTAG 3 cut(s) 1062, 1506, 1736
BfmI CTRYAG 4 cut(s) 91, 606, 1262, 1542
BfrI CTTAAG 1 cut(s) 1148
BfuAI ACCTGC 1 cut(s) 442
BfuI GTATCC 1 cut(s) 971
BglI GCCNNNNNGGC 1 cut(s) 505
BisI GCNGC 7 cut(s) 617, 800, 824, 1262, 1265, 1610, 1613
BlsI GCNGC 7 cut(s) 618, 801, 825, 1263, 1266, 1611, 1614
Bme1390I CCNGG 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 1600
BmeT110I CYCGRG 3 cut(s) 188, 620, 878
BmgT120I GGNCC 2 cut(s) 1075, 1600
BmrFI CCNGG 1 cut(s) 47
BmrI ACTGGG 1 cut(s) 1709
BmsI GCATC 5 cut(s) 130, 219, 355, 810, 1680
BmuI ACTGGG 1 cut(s) 1709
BpiI GAAGAC 5 cut(s) 240, 335, 585, 1346, 1520
BplI GAGNNNNNCTC 2 cut(s) 923, 955
BpmI CTGGAG 3 cut(s) 1160, 1289, 1443
Bpu10I CCTNAGC 4 cut(s) 509, 1004, 1467, 1556
Bpu14I TTCGAA 1 cut(s) 530
BpuEI CTTGAG 3 cut(s) 251, 827, 935
BsaI GGTCTC 4 cut(s) 26, 1387, 1616, 1709
BsaJI CCNNGG 2 cut(s) 760, 1344
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 1262, 1292
Bsc4I CCNNNNNNNGG 5 cut(s) 229, 322, 573, 955, 982
Bse1I ACTGG 3 cut(s) 1105, 1426, 1704
Bse3DI GCAATG 1 cut(s) 136
BseAI TCCGGA 1 cut(s) 213
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 2 cut(s) 760, 1344
BseGI GGATG 6 cut(s) 210, 219, 403, 1071, 1279, 1695
BseLI CCNNNNNNNGG 5 cut(s) 229, 322, 573, 955, 982
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 6 cut(s) 59, 500, 1018, 1481, 1547, 1720
BseNI ACTGG 3 cut(s) 1105, 1426, 1704
BseRI GAGGAG 9 cut(s) 536, 551, 755, 926, 1298, 1301, 1319, 1565, 1781
BseXI GCAGC 6 cut(s) 628, 810, 1248, 1276, 1596, 1599
BseYI CCCAGC 2 cut(s) 179, 1673
Bsh1236I CGCG 2 cut(s) 117, 814
BshFI GGCC 1 cut(s) 1077
BsiHKAI GWGCWC 1 cut(s) 658
BsiHKCI CYCGRG 3 cut(s) 188, 620, 878
BsiSI CCGG 3 cut(s) 214, 518, 752
BslFI GGGAC 1 cut(s) 1493
BslI CCNNNNNNNGG 5 cut(s) 229, 322, 573, 955, 982
BsmAI GTCTC 6 cut(s) 26, 198, 1387, 1565, 1616, 1709
BsmFI GGGAC 1 cut(s) 1493
BsmI GAATGC 2 cut(s) 41, 286
BsnI GGCC 1 cut(s) 1077
Bso31I GGTCTC 4 cut(s) 26, 1387, 1616, 1709
BsoBI CYCGRG 3 cut(s) 188, 620, 878
Bsp119I TTCGAA 1 cut(s) 530
Bsp1286I GDGCHC 1 cut(s) 658
Bsp13I TCCGGA 1 cut(s) 213
Bsp143I GATC 9 cut(s) 54, 75, 153, 175, 216, 664, 793, 1455, 1657
BspACI CCGC 4 cut(s) 50, 115, 800, 1626
BspANI GGCC 1 cut(s) 1077
BspCNI CTCAG 6 cut(s) 60, 501, 1017, 1480, 1548, 1719
BspEI TCCGGA 1 cut(s) 213
BspFNI CGCG 2 cut(s) 117, 814
BspHI TCATGA 1 cut(s) 1458
BspMAI CTGCAG 1 cut(s) 1266
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 5 cut(s) 148, 170, 224, 1463, 1652
BspT104I TTCGAA 1 cut(s) 530
BspTI CTTAAG 1 cut(s) 1148
BspTNI GGTCTC 4 cut(s) 26, 1387, 1616, 1709
BsrBI CCGCTC 1 cut(s) 802
BsrDI GCAATG 1 cut(s) 136
BsrI ACTGG 3 cut(s) 1105, 1426, 1704
BssECI CCNNGG 2 cut(s) 760, 1344
BssMI GATC 9 cut(s) 54, 75, 153, 175, 216, 664, 793, 1455, 1657
BssNAI GTATAC 1 cut(s) 918
BssSI CACGAG 1 cut(s) 676
BssT1I CCWWGG 2 cut(s) 760, 1344
Bst1107I GTATAC 1 cut(s) 918
Bst2BI CACGAG 1 cut(s) 676
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 4 cut(s) 334, 607, 1212, 1407
Bst6I CTCTTC 3 cut(s) 384, 644, 1556
BstAFI CTTAAG 1 cut(s) 1148
BstBI TTCGAA 1 cut(s) 530
BstC8I GCNNGC 3 cut(s) 119, 185, 993
BstDEI CTNAG 6 cut(s) 68, 509, 1004, 1467, 1556, 1706
BstF5I GGATG 6 cut(s) 210, 219, 403, 1071, 1279, 1695
BstFNI CGCG 2 cut(s) 117, 814
BstHHI GCGC 1 cut(s) 714
BstKTI GATC 9 cut(s) 57, 78, 156, 178, 219, 667, 796, 1458, 1660
BstMAI GTCTC 6 cut(s) 26, 198, 1387, 1565, 1616, 1709
BstMBI GATC 9 cut(s) 54, 75, 153, 175, 216, 664, 793, 1455, 1657
BstMWI GCNNNNNNNGC 8 cut(s) 47, 140, 271, 505, 514, 709, 820, 992
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 2 cut(s) 1504, 1756
BstSCI CCNGG 1 cut(s) 45
BstSFI CTRYAG 4 cut(s) 91, 606, 1262, 1542
BstUI CGCG 2 cut(s) 117, 814
BstV1I GCAGC 6 cut(s) 628, 810, 1248, 1276, 1596, 1599
BstV2I GAAGAC 5 cut(s) 240, 335, 585, 1346, 1520
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BstZ17I GTATAC 1 cut(s) 918
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 1 cut(s) 1077
BtsCI GGATG 6 cut(s) 210, 219, 403, 1071, 1279, 1695
BtsIMutI CAGTG 2 cut(s) 603, 1026
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 3 cut(s) 119, 185, 993
CaiI CAGNNNCTG 1 cut(s) 67
CciI TCATGA 1 cut(s) 1458
CfoI GCGC 1 cut(s) 714
Cfr13I GGNCC 2 cut(s) 1075, 1600
Csp6I GTAC 3 cut(s) 467, 757, 1009
CviAII CATG 7 cut(s) 470, 965, 1447, 1459, 1489, 1501, 1753
CviQI GTAC 3 cut(s) 467, 757, 1009
DdeI CTNAG 6 cut(s) 68, 509, 1004, 1467, 1556, 1706
DpnI GATC 9 cut(s) 56, 77, 155, 177, 218, 666, 795, 1457, 1659
DpnII GATC 9 cut(s) 54, 75, 153, 175, 216, 664, 793, 1455, 1657
DraIII CACNNNGTG 1 cut(s) 1409
Eam1104I CTCTTC 3 cut(s) 384, 644, 1556
EarI CTCTTC 3 cut(s) 384, 644, 1556
EciI GGCGGA 1 cut(s) 65
Eco130I CCWWGG 2 cut(s) 760, 1344
Eco31I GGTCTC 4 cut(s) 26, 1387, 1616, 1709
Eco47I GGWCC 1 cut(s) 1600
Eco88I CYCGRG 3 cut(s) 188, 620, 878
EcoO109I RGGNCCY 1 cut(s) 1600
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 2 cut(s) 760, 1344
EcoT22I ATGCAT 2 cut(s) 145, 212
ErhI CCWWGG 2 cut(s) 760, 1344
FaeI CATG 7 cut(s) 473, 968, 1450, 1462, 1492, 1504, 1756
FalI AAGNNNNNCTT 4 cut(s) 570, 602, 643, 675
FaqI GGGAC 1 cut(s) 1493
FatI CATG 7 cut(s) 469, 964, 1446, 1458, 1488, 1500, 1752
FbaI TGATCA 1 cut(s) 75
FblI GTMKAC 2 cut(s) 917, 1749
Fnu4HI GCNGC 7 cut(s) 617, 800, 824, 1262, 1265, 1610, 1613
FokI GGATG 6 cut(s) 197, 206, 410, 1078, 1286, 1702
Fsp4HI GCNGC 7 cut(s) 617, 800, 824, 1262, 1265, 1610, 1613
FspBI CTAG 3 cut(s) 1062, 1506, 1736
FspI TGCGCA 1 cut(s) 713
GlaI GCGC 1 cut(s) 713
GluI GCNGC 7 cut(s) 617, 800, 824, 1262, 1265, 1610, 1613
GsaI CCCAGC 2 cut(s) 183, 1677
GsuI CTGGAG 3 cut(s) 1160, 1289, 1443
HaeIII GGCC 1 cut(s) 1077
HapII CCGG 3 cut(s) 214, 518, 752
HhaI GCGC 1 cut(s) 714
Hin1II CATG 7 cut(s) 473, 968, 1450, 1462, 1492, 1504, 1756
Hin6I GCGC 1 cut(s) 712
HinP1I GCGC 1 cut(s) 712
HincII GTYRAC 2 cut(s) 1750, 1804
HindII GTYRAC 2 cut(s) 1750, 1804
HindIII AAGCTT 2 cut(s) 701, 1235
HpaII CCGG 3 cut(s) 214, 518, 752
HphI GGTGA 7 cut(s) 466, 859, 877, 1115, 1395, 1429, 1606
Hpy166II GTNNAC 5 cut(s) 918, 1399, 1437, 1750, 1804
Hpy188I TCNGA 8 cut(s) 69, 139, 158, 292, 790, 1282, 1387, 1765
Hpy188III TCNNGA 9 cut(s) 163, 214, 880, 940, 1123, 1139, 1459, 1532, 1570
Hpy8I GTNNAC 5 cut(s) 918, 1399, 1437, 1750, 1804
HpyCH4III ACNGT 4 cut(s) 334, 607, 1212, 1407
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 8 cut(s) 143, 210, 368, 546, 826, 899, 1264, 1335
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 140, 271, 505, 514, 709, 820, 992
HpyF3I CTNAG 6 cut(s) 68, 509, 1004, 1467, 1556, 1706
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 7 cut(s) 473, 968, 1450, 1462, 1492, 1504, 1756
HspAI GCGC 1 cut(s) 712
Kpn2I TCCGGA 1 cut(s) 213
Ksp22I TGATCA 1 cut(s) 75
Kzo9I GATC 9 cut(s) 54, 75, 153, 175, 216, 664, 793, 1455, 1657
LmnI GCTCC 5 cut(s) 271, 478, 742, 1493, 1552
Lsp1109I GCAGC 6 cut(s) 628, 810, 1248, 1276, 1596, 1599
LweI GCATC 5 cut(s) 130, 219, 355, 810, 1680
MaeI CTAG 3 cut(s) 1062, 1506, 1736
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 3 cut(s) 1057, 1246, 1417
MalI GATC 9 cut(s) 56, 77, 155, 177, 218, 666, 795, 1457, 1659
MbiI CCGCTC 1 cut(s) 802
MboI GATC 9 cut(s) 54, 75, 153, 175, 216, 664, 793, 1455, 1657
MfeI CAATTG 1 cut(s) 733
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 658
MluCI AATT 3 cut(s) 9, 733, 923
MlyI GAGTC 3 cut(s) 200, 1286, 1394
MmeI TCCRAC 2 cut(s) 1186, 1260
Mph1103I ATGCAT 2 cut(s) 145, 212
MroI TCCGGA 1 cut(s) 213
MroXI GAANNNNTTC 1 cut(s) 599
MseI TTAA 3 cut(s) 1047, 1149, 1527
MslI CAYNNNNRTG 1 cut(s) 1064
MspA1I CMGCKG 1 cut(s) 1267
MspCI CTTAAG 1 cut(s) 1148
MspI CCGG 3 cut(s) 214, 518, 752
MspR9I CCNGG 1 cut(s) 47
MunI CAATTG 1 cut(s) 733
Mva1269I GAATGC 2 cut(s) 41, 286
MvaI CCWGG 1 cut(s) 47
MvnI CGCG 2 cut(s) 117, 814
MwoI GCNNNNNNNGC 8 cut(s) 47, 140, 271, 505, 514, 709, 820, 992
NdeII GATC 9 cut(s) 54, 75, 153, 175, 216, 664, 793, 1455, 1657
NlaIII CATG 7 cut(s) 473, 968, 1450, 1462, 1492, 1504, 1756
NmuCI GTSAC 2 cut(s) 1057, 1417
NsbI TGCGCA 1 cut(s) 713
NsiI ATGCAT 2 cut(s) 145, 212
NspI RCATGY 2 cut(s) 1504, 1756
NspV TTCGAA 1 cut(s) 530
PaeR7I CTCGAG 3 cut(s) 188, 620, 878
PagI TCATGA 1 cut(s) 1458
PaqCI CACCTGC 1 cut(s) 442
PciI ACATGT 1 cut(s) 1500
PctI GAATGC 2 cut(s) 41, 286
PdmI GAANNNNTTC 1 cut(s) 599
PfeI GAWTC 7 cut(s) 159, 785, 838, 859, 1327, 1351, 1681
PflFI GACNNNGTC 1 cut(s) 1055
PflMI CCANNNNNTGG 1 cut(s) 573
PkrI GCNGC 7 cut(s) 618, 801, 825, 1263, 1266, 1611, 1614
PleI GAGTC 3 cut(s) 199, 1285, 1394
PpsI GAGTC 3 cut(s) 199, 1285, 1394
PpuMI RGGWCCY 1 cut(s) 1600
PscI ACATGT 1 cut(s) 1500
PshBI ATTAAT 1 cut(s) 1527
PsiI TTATAA 2 cut(s) 299, 725
Psp5II RGGWCCY 1 cut(s) 1600
Psp6I CCWGG 1 cut(s) 45
PspFI CCCAGC 2 cut(s) 179, 1673
PspGI CCWGG 1 cut(s) 45
PspPI GGNCC 2 cut(s) 1075, 1600
PspPPI RGGWCCY 1 cut(s) 1600
PspXI VCTCGAGB 2 cut(s) 188, 620
PsrI GAACNNNNNNTAC 2 cut(s) 1076, 1108
PstI CTGCAG 1 cut(s) 1266
PstNI CAGNNNCTG 1 cut(s) 67
PsuI RGATCY 1 cut(s) 153
PsyI GACNNNGTC 1 cut(s) 1055
PvuII CAGCTG 1 cut(s) 1267
RsaI GTAC 3 cut(s) 468, 758, 1010
RsaNI GTAC 3 cut(s) 467, 757, 1009
RseI CAYNNNNRTG 1 cut(s) 1064
SalI GTCGAC 1 cut(s) 1748
SaqAI TTAA 3 cut(s) 1047, 1149, 1527
SatI GCNGC 7 cut(s) 617, 800, 824, 1262, 1265, 1610, 1613
Sau3AI GATC 9 cut(s) 54, 75, 153, 175, 216, 664, 793, 1455, 1657
Sau96I GGNCC 2 cut(s) 1075, 1600
SchI GAGTC 3 cut(s) 200, 1286, 1394
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 1 cut(s) 658
SfaNI GCATC 5 cut(s) 130, 219, 355, 810, 1680
SfcI CTRYAG 4 cut(s) 91, 606, 1262, 1542
Sfr274I CTCGAG 3 cut(s) 188, 620, 878
SfuI TTCGAA 1 cut(s) 530
SinI GGWCC 1 cut(s) 1600
SlaI CTCGAG 3 cut(s) 188, 620, 878
SmiMI CAYNNNNRTG 1 cut(s) 1064
SmlI CTYRAG 7 cut(s) 188, 266, 620, 842, 878, 950, 1148
SmoI CTYRAG 7 cut(s) 188, 266, 620, 842, 878, 950, 1148
SpeI ACTAGT 1 cut(s) 1735
Sse9I AATT 3 cut(s) 9, 733, 923
SsiI CCGC 4 cut(s) 50, 115, 800, 1626
SspMI CTAG 3 cut(s) 1062, 1506, 1736
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 2 cut(s) 760, 1344
TaaI ACNGT 4 cut(s) 334, 607, 1212, 1407
TaiI ACGT 1 cut(s) 30
TasI AATT 3 cut(s) 9, 733, 923
TauI GCSGC 1 cut(s) 802
TfiI GAWTC 7 cut(s) 159, 785, 838, 859, 1327, 1351, 1681
Tru1I TTAA 3 cut(s) 1047, 1149, 1527
Tru9I TTAA 3 cut(s) 1047, 1149, 1527
TscAI CASTG 2 cut(s) 610, 1033
TseFI GTSAC 2 cut(s) 1057, 1417
TseI GCWGC 6 cut(s) 616, 823, 1261, 1264, 1609, 1612
Tsp45I GTSAC 2 cut(s) 1057, 1417
TspDTI ATGAA 4 cut(s) 372, 1082, 1475, 1731
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 2 cut(s) 610, 1033
Tth111I GACNNNGTC 1 cut(s) 1055
Van91I CCANNNNNTGG 1 cut(s) 573
Vha464I CTTAAG 1 cut(s) 1148
VpaK11BI GGWCC 1 cut(s) 1600
VspI ATTAAT 1 cut(s) 1527
XapI RAATTY 1 cut(s) 923
XceI RCATGY 2 cut(s) 1504, 1756
XhoI CTCGAG 3 cut(s) 188, 620, 878
XmiI GTMKAC 2 cut(s) 917, 1749
XmnI GAANNNNTTC 1 cut(s) 599
XspI CTAG 3 cut(s) 1062, 1506, 1736
Zsp2I ATGCAT 2 cut(s) 145, 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.