Heat shock protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 207 |
| AciI | CCGC | 4 cut(s) | 48, 158, 214, 339 |
| AclWI | GGATC | 2 cut(s) | 232, 612 |
| AcsI | RAATTY | 2 cut(s) | 725, 824 |
| AcuI | CTGAAG | 2 cut(s) | 455, 614 |
| AcyI | GRCGYC | 1 cut(s) | 328 |
| AfaI | GTAC | 1 cut(s) | 324 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 345, 459 |
| AflIII | ACRYGT | 1 cut(s) | 225 |
| AgsI | TTSAA | 8 cut(s) | 124, 345, 410, 467, 500, 524, 559, 568 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 226 |
| AjiI | CACGTC | 1 cut(s) | 850 |
| AjnI | CCWGG | 1 cut(s) | 803 |
| AluBI | AGCT | 4 cut(s) | 127, 537, 598, 737 |
| AluI | AGCT | 4 cut(s) | 127, 537, 598, 737 |
| Alw26I | GTCTC | 3 cut(s) | 335, 469, 681 |
| AlwI | GGATC | 2 cut(s) | 232, 612 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 478 |
| AoxI | GGCC | 2 cut(s) | 336, 801 |
| ApeKI | GCWGC | 5 cut(s) | 11, 481, 598, 691, 734 |
| ApoI | RAATTY | 2 cut(s) | 725, 824 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 150, 165, 356, 563 |
| AspLEI | GCGC | 1 cut(s) | 207 |
| AspS9I | GGNCC | 1 cut(s) | 336 |
| AsuHPI | GGTGA | 2 cut(s) | 302, 671 |
| AxyI | CCTNAGG | 1 cut(s) | 885 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 306, 338 |
| BbvCI | CCTCAGC | 1 cut(s) | 396 |
| BbvI | GCAGC | 5 cut(s) | 23, 493, 585, 703, 746 |
| BccI | CCATC | 3 cut(s) | 67, 707, 793 |
| BceAI | ACGGC | 1 cut(s) | 341 |
| BciT130I | CCWGG | 1 cut(s) | 805 |
| BciVI | GTATCC | 1 cut(s) | 115 |
| BclI | TGATCA | 1 cut(s) | 292 |
| BcoDI | GTCTC | 3 cut(s) | 335, 469, 681 |
| BfaI | CTAG | 1 cut(s) | 311 |
| BfmI | CTRYAG | 2 cut(s) | 479, 732 |
| BfuI | GTATCC | 1 cut(s) | 115 |
| BisI | GCNGC | 6 cut(s) | 12, 339, 482, 599, 692, 735 |
| BlsI | GCNGC | 6 cut(s) | 13, 340, 483, 600, 693, 736 |
| Bme1390I | CCNGG | 1 cut(s) | 805 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 226 |
| BmgBI | CACGTC | 1 cut(s) | 850 |
| BmgT120I | GGNCC | 1 cut(s) | 336 |
| BmrFI | CCNGG | 1 cut(s) | 805 |
| BmsI | GCATC | 3 cut(s) | 382, 536, 649 |
| Bpu10I | CCTNAGC | 1 cut(s) | 396 |
| BpuEI | CTTGAG | 1 cut(s) | 254 |
| Bsa29I | ATCGAT | 1 cut(s) | 189 |
| BsaHI | GRCGYC | 1 cut(s) | 328 |
| BsaI | GGTCTC | 1 cut(s) | 681 |
| BsaJI | CCNNGG | 1 cut(s) | 829 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 74, 104 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 345, 459 |
| Bse21I | CCTNAGG | 1 cut(s) | 885 |
| Bse3DI | GCAATG | 2 cut(s) | 281, 464 |
| BseBI | CCWGG | 1 cut(s) | 805 |
| BseCI | ATCGAT | 1 cut(s) | 189 |
| BseDI | CCNNGG | 1 cut(s) | 829 |
| BseGI | GGATG | 2 cut(s) | 59, 813 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 345, 459 |
| BseMI | GCAATG | 2 cut(s) | 281, 464 |
| BseMII | CTCAG | 3 cut(s) | 387, 420, 876 |
| BseRI | GAGGAG | 4 cut(s) | 82, 85, 413, 808 |
| BseXI | GCAGC | 5 cut(s) | 23, 493, 585, 703, 746 |
| Bsh1236I | CGCG | 1 cut(s) | 207 |
| BshFI | GGCC | 2 cut(s) | 338, 803 |
| BshVI | ATCGAT | 1 cut(s) | 189 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 345, 459 |
| BsmAI | GTCTC | 3 cut(s) | 335, 469, 681 |
| BsmBI | CGTCTC | 1 cut(s) | 335 |
| BsmI | GAATGC | 1 cut(s) | 694 |
| BsnI | GGCC | 2 cut(s) | 338, 803 |
| Bso31I | GGTCTC | 1 cut(s) | 681 |
| Bsp143I | GATC | 4 cut(s) | 190, 237, 292, 617 |
| BspACI | CCGC | 4 cut(s) | 48, 158, 214, 339 |
| BspANI | GGCC | 2 cut(s) | 338, 803 |
| BspCNI | CTCAG | 3 cut(s) | 388, 421, 877 |
| BspDI | ATCGAT | 1 cut(s) | 189 |
| BspFNI | CGCG | 1 cut(s) | 207 |
| BspMAI | CTGCAG | 2 cut(s) | 483, 736 |
| BspPI | GGATC | 2 cut(s) | 232, 612 |
| BspQI | GCTCTTC | 1 cut(s) | 495 |
| BspTNI | GGTCTC | 1 cut(s) | 681 |
| BsrDI | GCAATG | 2 cut(s) | 281, 464 |
| BssECI | CCNNGG | 1 cut(s) | 829 |
| BssMI | GATC | 4 cut(s) | 190, 237, 292, 617 |
| BssNI | GRCGYC | 1 cut(s) | 328 |
| BssT1I | CCWWGG | 1 cut(s) | 829 |
| Bst2UI | CCWGG | 1 cut(s) | 805 |
| Bst6I | CTCTTC | 3 cut(s) | 41, 312, 495 |
| BstACI | GRCGYC | 1 cut(s) | 328 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 392, 740 |
| BstC8I | GCNNGC | 1 cut(s) | 486 |
| BstDEI | CTNAG | 4 cut(s) | 396, 423, 429, 885 |
| BstEII | GGTNACC | 2 cut(s) | 415, 448 |
| BstF5I | GGATG | 2 cut(s) | 59, 813 |
| BstFNI | CGCG | 1 cut(s) | 207 |
| BstHHI | GCGC | 1 cut(s) | 207 |
| BstKTI | GATC | 4 cut(s) | 193, 240, 295, 620 |
| BstMAI | GTCTC | 3 cut(s) | 335, 469, 681 |
| BstMBI | GATC | 4 cut(s) | 190, 237, 292, 617 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 211, 392, 598, 740 |
| BstNI | CCWGG | 1 cut(s) | 805 |
| BstNSI | RCATGY | 1 cut(s) | 229 |
| BstPI | GGTNACC | 2 cut(s) | 415, 448 |
| BstSCI | CCNGG | 1 cut(s) | 803 |
| BstSFI | CTRYAG | 2 cut(s) | 479, 732 |
| BstUI | CGCG | 1 cut(s) | 207 |
| BstV1I | GCAGC | 5 cut(s) | 23, 493, 585, 703, 746 |
| BstX2I | RGATCY | 2 cut(s) | 237, 617 |
| BstYI | RGATCY | 2 cut(s) | 237, 617 |
| Bsu15I | ATCGAT | 1 cut(s) | 189 |
| Bsu36I | CCTNAGG | 1 cut(s) | 885 |
| BsuI | GTATCC | 1 cut(s) | 115 |
| BsuRI | GGCC | 2 cut(s) | 338, 803 |
| BsuTUI | ATCGAT | 1 cut(s) | 189 |
| BtrI | CACGTC | 1 cut(s) | 850 |
| BtsCI | GGATG | 2 cut(s) | 59, 813 |
| Cac8I | GCNNGC | 1 cut(s) | 486 |
| CaiI | CAGNNNCTG | 1 cut(s) | 478 |
| CfoI | GCGC | 1 cut(s) | 207 |
| Cfr13I | GGNCC | 1 cut(s) | 336 |
| ClaI | ATCGAT | 1 cut(s) | 189 |
| CseI | GACGC | 1 cut(s) | 317 |
| Csp6I | GTAC | 1 cut(s) | 323 |
| CviAII | CATG | 4 cut(s) | 226, 287, 368, 445 |
| CviQI | GTAC | 1 cut(s) | 323 |
| DdeI | CTNAG | 4 cut(s) | 396, 423, 429, 885 |
| DpnI | GATC | 4 cut(s) | 192, 239, 294, 619 |
| DpnII | GATC | 4 cut(s) | 190, 237, 292, 617 |
| DriI | GACNNNNNGTC | 1 cut(s) | 226 |
| Eam1104I | CTCTTC | 3 cut(s) | 41, 312, 495 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 226 |
| EarI | CTCTTC | 3 cut(s) | 41, 312, 495 |
| Eco130I | CCWWGG | 1 cut(s) | 829 |
| Eco31I | GGTCTC | 1 cut(s) | 681 |
| Eco57I | CTGAAG | 2 cut(s) | 455, 614 |
| Eco81I | CCTNAGG | 1 cut(s) | 885 |
| Eco91I | GGTNACC | 2 cut(s) | 415, 448 |
| EcoO65I | GGTNACC | 2 cut(s) | 415, 448 |
| EcoRII | CCWGG | 1 cut(s) | 803 |
| EcoT14I | CCWWGG | 1 cut(s) | 829 |
| ErhI | CCWWGG | 1 cut(s) | 829 |
| Esp3I | CGTCTC | 1 cut(s) | 335 |
| FaeI | CATG | 4 cut(s) | 229, 290, 371, 448 |
| FatI | CATG | 4 cut(s) | 225, 286, 367, 444 |
| FauI | CCCGC | 1 cut(s) | 55 |
| FbaI | TGATCA | 1 cut(s) | 292 |
| Fnu4HI | GCNGC | 6 cut(s) | 12, 339, 482, 599, 692, 735 |
| FokI | GGATG | 2 cut(s) | 46, 820 |
| Fsp4HI | GCNGC | 6 cut(s) | 12, 339, 482, 599, 692, 735 |
| FspBI | CTAG | 1 cut(s) | 311 |
| GlaI | GCGC | 1 cut(s) | 206 |
| GluI | GCNGC | 6 cut(s) | 12, 339, 482, 599, 692, 735 |
| HaeIII | GGCC | 2 cut(s) | 338, 803 |
| HgaI | GACGC | 1 cut(s) | 317 |
| HhaI | GCGC | 1 cut(s) | 207 |
| Hin1I | GRCGYC | 1 cut(s) | 328 |
| Hin1II | CATG | 4 cut(s) | 229, 290, 371, 448 |
| Hin6I | GCGC | 1 cut(s) | 205 |
| HinP1I | GCGC | 1 cut(s) | 205 |
| HinfI | GANTC | 6 cut(s) | 86, 146, 172, 186, 463, 654 |
| HphI | GGTGA | 2 cut(s) | 302, 671 |
| Hpy188I | TCNGA | 4 cut(s) | 70, 171, 177, 474 |
| Hpy188III | TCNNGA | 5 cut(s) | 311, 556, 578, 630, 884 |
| Hpy99I | CGWCG | 2 cut(s) | 86, 104 |
| HpyAV | CCTTC | 1 cut(s) | 584 |
| HpyCH4IV | ACGT | 2 cut(s) | 612, 849 |
| HpyCH4V | TGCA | 9 cut(s) | 250, 286, 376, 481, 549, 640, 691, 734, 862 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 211, 392, 598, 740 |
| HpyF3I | CTNAG | 4 cut(s) | 396, 423, 429, 885 |
| HpySE526I | ACGT | 2 cut(s) | 612, 849 |
| Hsp92I | GRCGYC | 1 cut(s) | 328 |
| Hsp92II | CATG | 4 cut(s) | 229, 290, 371, 448 |
| HspAI | GCGC | 1 cut(s) | 205 |
| Ksp22I | TGATCA | 1 cut(s) | 292 |
| Kzo9I | GATC | 4 cut(s) | 190, 237, 292, 617 |
| LguI | GCTCTTC | 1 cut(s) | 495 |
| LpnPI | CCDG | 5 cut(s) | 319, 470, 650, 790, 817 |
| Lsp1109I | GCAGC | 5 cut(s) | 23, 493, 585, 703, 746 |
| LweI | GCATC | 3 cut(s) | 382, 536, 649 |
| MaeI | CTAG | 1 cut(s) | 311 |
| MaeII | ACGT | 2 cut(s) | 612, 849 |
| MaeIII | GTNAC | 3 cut(s) | 415, 448, 850 |
| MalI | GATC | 4 cut(s) | 192, 239, 294, 619 |
| MboI | GATC | 4 cut(s) | 190, 237, 292, 617 |
| MboII | GAAGA | 9 cut(s) | 28, 247, 326, 329, 361, 445, 482, 556, 730 |
| MfeI | CAATTG | 1 cut(s) | 644 |
| MflI | RGATCY | 2 cut(s) | 237, 617 |
| MluCI | AATT | 7 cut(s) | 15, 119, 355, 644, 725, 752, 824 |
| MroXI | GAANNNNTTC | 4 cut(s) | 150, 165, 356, 563 |
| MseI | TTAA | 2 cut(s) | 303, 669 |
| MspR9I | CCNGG | 1 cut(s) | 805 |
| MunI | CAATTG | 1 cut(s) | 644 |
| Mva1269I | GAATGC | 1 cut(s) | 694 |
| MvaI | CCWGG | 1 cut(s) | 805 |
| MvnI | CGCG | 1 cut(s) | 207 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 211, 392, 598, 740 |
| NdeII | GATC | 4 cut(s) | 190, 237, 292, 617 |
| NlaIII | CATG | 4 cut(s) | 229, 290, 371, 448 |
| NmuCI | GTSAC | 1 cut(s) | 850 |
| NspI | RCATGY | 1 cut(s) | 229 |
| PciI | ACATGT | 1 cut(s) | 225 |
| PciSI | GCTCTTC | 1 cut(s) | 495 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 195, 204 |
| PctI | GAATGC | 1 cut(s) | 694 |
| PdmI | GAANNNNTTC | 4 cut(s) | 150, 165, 356, 563 |
| PfeI | GAWTC | 6 cut(s) | 86, 146, 172, 186, 463, 654 |
| PkrI | GCNGC | 6 cut(s) | 13, 340, 483, 600, 693, 736 |
| PscI | ACATGT | 1 cut(s) | 225 |
| Psp6I | CCWGG | 1 cut(s) | 803 |
| PspEI | GGTNACC | 2 cut(s) | 415, 448 |
| PspGI | CCWGG | 1 cut(s) | 803 |
| PspPI | GGNCC | 1 cut(s) | 336 |
| PstI | CTGCAG | 2 cut(s) | 483, 736 |
| PstNI | CAGNNNCTG | 1 cut(s) | 478 |
| PsuI | RGATCY | 2 cut(s) | 237, 617 |
| RsaI | GTAC | 1 cut(s) | 324 |
| RsaNI | GTAC | 1 cut(s) | 323 |
| SapI | GCTCTTC | 1 cut(s) | 495 |
| SaqAI | TTAA | 2 cut(s) | 303, 669 |
| SatI | GCNGC | 6 cut(s) | 12, 339, 482, 599, 692, 735 |
| Sau3AI | GATC | 4 cut(s) | 190, 237, 292, 617 |
| Sau96I | GGNCC | 1 cut(s) | 336 |
| ScrFI | CCNGG | 1 cut(s) | 805 |
| SfaNI | GCATC | 3 cut(s) | 382, 536, 649 |
| SfcI | CTRYAG | 2 cut(s) | 479, 732 |
| SmlI | CTYRAG | 1 cut(s) | 269 |
| SmoI | CTYRAG | 1 cut(s) | 269 |
| Sse9I | AATT | 7 cut(s) | 15, 119, 355, 644, 725, 752, 824 |
| SsiI | CCGC | 4 cut(s) | 48, 158, 214, 339 |
| SspMI | CTAG | 1 cut(s) | 311 |
| StyD4I | CCNGG | 1 cut(s) | 803 |
| StyI | CCWWGG | 1 cut(s) | 829 |
| TaiI | ACGT | 2 cut(s) | 615, 852 |
| TaqI | TCGA | 4 cut(s) | 189, 198, 360, 506 |
| TasI | AATT | 7 cut(s) | 15, 119, 355, 644, 725, 752, 824 |
| TauI | GCSGC | 1 cut(s) | 341 |
| TfiI | GAWTC | 6 cut(s) | 86, 146, 172, 186, 463, 654 |
| Tru1I | TTAA | 2 cut(s) | 303, 669 |
| Tru9I | TTAA | 2 cut(s) | 303, 669 |
| TseFI | GTSAC | 1 cut(s) | 850 |
| TseI | GCWGC | 5 cut(s) | 11, 481, 598, 691, 734 |
| Tsp45I | GTSAC | 1 cut(s) | 850 |
| TspDTI | ATGAA | 5 cut(s) | 8, 356, 452, 482, 738 |
| TspGWI | ACGGA | 1 cut(s) | 88 |
| XapI | RAATTY | 2 cut(s) | 725, 824 |
| XbaI | TCTAGA | 1 cut(s) | 310 |
| XceI | RCATGY | 1 cut(s) | 229 |
| XmnI | GAANNNNTTC | 4 cut(s) | 150, 165, 356, 563 |
| XspI | CTAG | 1 cut(s) | 311 |