RchiOBHm_Chr6g0274441

Heat shock protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000335
Physical Location & Seq
Forward (+)
35930252 .. 35933430
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24619

Sequence Viewer

Length: 2106 bp
ATGGCGGATGCGGAGACCTTTGCGTTCCAGGCCGAGATCAACCAGCTTCTGAGTCTGATCATCAACACTTTTTACAGCAACAAGGAGATTTTCCTCCGCGAAATCATCAGCAATTCCTCTGATGCTTTGGACAAAATTCGATTCGAGAGCCTTACGGACAAAAGCAAGCTTGATGCTCAACCGGAGCTTTTCATCAGGCTTGTGCCTGATAAGGTCAACAACACGCTCTCCATCATTGATAGTGGCATTGGCATGACCAAAGCAGATTTGGTGAACAACTTGGGCACGATTGCAAGGTCCGGAACCAAGGAGTTTATGGAGGCCTTGCAGTCTGGGGTGTCTGATGTGAGCATGATTGGACAGTTTGGTGTGGGTTTCTATTCCGCTTATCTTGTTGCTGAGAAAGTCATTGTGACCACAAAACACAATGATGATGAACAATACATCTGGGAATCGCAAGCGGGTGGTTCCTTCACTGTCACTAGAGATGTAAACGGAGAGCAGCTAGGCAGGGGAACCAAGATCACTCTCTTCCTCAAGGAGGATCAGTTGGAATATCTGGAGGAAAGGAGGATCAAGGACCTTGTGAAGAAGCACTCTGAATTCATCAGCTATCCCATTTATCTTTGGACGGAGAAGACTACTGAGAAAGAGATTAGTGATGATGAGGAAGATGAGGAAGCCAAGAAGGAAGAGGAAGGAGAAGTTGAGGAAGTTAACGAGGACAAGGAGAAAGAATCAAAGAAGAAAAAGAAGATCAAGGAGGTTTCCCACGAGTGGGAGCTAATCAACAAGCAGAAACCTATCTGGCTGCGCAAGCCCGAGGAAATTACCAAGGATGAATATGCTGCATTCTACAAGAGCTTGACCAATGACTGGGAGGAACACCTTTCTGTCAAGCATTTCTCTGTTGAAGGACAGCTTGAATTCAAAGCCATTCTCTTTGTCCCAAAGAGAGCTCCGTTCGACCTGTTTGACACCAGGAAAAAGATGAATAACATTAAGCTCTATGTCAGGAGAGTGTTTATTATGGACAACTGTGAAGAGCTCATCCCAGAGTATCTTGGATTTGTTAAGGGTGTCGTGGACTCCGATGACTTGCCACTGAACATCTCTCGTGAGATGCTTCAACAGAACAAGATATTGAAGGTGATCAGGAAGAACCTTGTGAAGAAGTGCATTGAGATGTTCAATGAGATTGCTGAAAACAAGGAGGATTACACCAAGTTTTATGAGGCATTCTCCAAGAACTTGAAGTTGGGTATTCATGAAGACAGCCAGAACAGAGCCAAATTGGCTGACCTTCTCCGGTACCACTCCACCAAGAGTGGTGAGGACCTGACAAGCCTGAAGGACTATGTTACCAGAATGAAGGAGGGCCAAAAGGACATTTACTACATTACTGGTGAGAGCAAGAAGGCAGTGGAGAATTCTCCATTCTTGGAGCGCCTCAAGAAAAAGGGATACGAAGTGCTTTTCATGGTGGATGCGATCGATGAGTATGCTGTAGGTCAGTTGAAGGAGTATGATGGCAAGAAGTTGGTGTCTGCCACAAAGGAAGGATTGAAGCTTGATGATGAAACTGAGGAAGAGAAGCAGAAGAGAGAGGAGAAGAAGAAATCCTTTGAGAATCTTTGCAAGACAATCAAGGACATTTTGGGAGATAGGATTGAGAAGGTAGTAGTATCTGACAGAATTGTGGATTCGCCTTGCTGCTTGGTGACGGGTGAATATGGCTGGACTGCGAATATGGAGAGGATCATGAAGGCTCAGGCTCTCAGGGACAACAGCATGGGATCCTACATGTCTAGCAAGAAGACAATGGAAATCAATCCAGATAATGGAATCATGGAGGAGTTGAGGAAGAGGGCCGAAGCTGATAAGAATGACAAGTCTGTCAAGGACCTTGTGCTGCTGCTGTTCGAGACTTCCCTCTTGACTTCCGGATTCAGCTTGGATGATCCCAACACTTTTGCGGCAAGAATCAACAGGATGTTGAAGCTGGGCCTCAGCATTGATGAGGATGAGGCAGCTGGTGAAGATGCAGATATGCCTGCTTTGGAGGACGATACTGCTGAAGAGAGCAAGATGGAGGAGGTCGACTAA

Protein Analysis

701

Amino Acids

80.66

Weight (kDa)

4.95

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HATPase_c PF02518 28 - 182 1.7e-07 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
HATPase_c_3 PF13589 31 - 147 1.2e-07 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
HSP90 PF00183 185 - 696 6.6e-242 Hsp90 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000335)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52640
fragaria_vesca FvH4_2g04730 FvH4_2g04730 FvH4_2g04730 FvH4_2g13180 FvH4_7g30260 FvH4_7g30272
malus_domestica MD00G1081900.v1.1 MD01G1208700.v1.1 MD07G1279100.v1.1 MD07G1279200.v1.1 MD11G1152300.v1.1 MD14G1110500.v1.1 MD14G1110600.v1.1
prunus_persica Prupe.2G301300_v2.0.a1 Prupe.5G041100_v2.0.a1 Prupe.5G105600_v2.0.a1
pyrus_communis pycom01g21930 pycom07g25400 pycom09g11140 pycom14g10530
rosa_chinensis RchiOBHm_Chr1g0327061 RchiOBHm_Chr1g0378391 RchiOBHm_Chr1g0379711 RchiOBHm_Chr3g0488481 RchiOBHm_Chr4g0387881 RchiOBHm_Chr5g0021331 RchiOBHm_Chr5g0021341 RchiOBHm_Chr5g0021351 RchiOBHm_Chr6g0274441 RchiOBHm_Chr7g0196751 RchiOBHm_Chr7g0196771
rosa_laevigata RLG00000003992 RLG00000008392 RLG00000009018 RLG00000013542 RLG00000026364 RLG00000026365 RLG00000026438 RLG00000032605
rosa_multiflora Rmu_co8309643.1_g000001 Rmu_co8423093.1_g000001 Rmu_co8520529.1_g000002 Rmu_sc0000938.1_g000001 Rmu_sc0001083.1_g000031 Rmu_sc0001289.1_g000016 Rmu_sc0001777.1_g000013 Rmu_sc0003392.1_g000006 Rmu_sc0003600.1_g000010 Rmu_sc0004484.1_g000024 Rmu_sc0004847.1_g000017 Rmu_sc0009850.1_g000016 Rmu_sc0013411.1_g000009 Rmu_sc0024337.1_g000001 Rmu_sc0024338.1_g000001 Rmu_sc0029385.1_g000001 Rmu_sc0039495.1_g000001 Rmu_ssc0000259.1_g000042
rosa_roxburghii Rroxscaffold_1G00057180 Rroxscaffold_3G00258970 Rroxscaffold_3G00258980 Rroxscaffold_4G00280480 Rroxscaffold_7G00194220 Rroxscaffold_7G00197660
rosa_rugosa Rorug01G0408500 Rorug05G0066400 Rorug05G0066500 Rorug06G0081900 Rorug07G0036200
rosa_samantha Rh1AG083900 Rh1AG430000 Rh1AG438200 Rh1AG438300 Rh1BG387700 Rh1BG394700 Rh1BG394800 Rh1CG081800 Rh1CG401200 Rh1CG407700 Rh1CG407800 Rh1DG327400 Rh1DG418800 Rh1DG425100 Rh1DG425200 Rh2AG541400 Rh2CG384100 Rh2CG524100 Rh4DG107200 Rh5AG155400 Rh5AG155500 Rh5AG155600 Rh5BG155000 Rh5BG155100 Rh5CG168500 Rh5CG168600 Rh5CG168700 Rh5DG155600 Rh6AG198300 Rh6BG201600 Rh6BG491300 Rh6CG202400 Rh6DG192800 Rh7AG160000 Rh7AG160100 Rh7BG162200 Rh7CG168600 Rh7DG161300
rosa_wichuraiana Rw1G037630 Rw5G013860 Rw6G017240 Rw7G013990

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1895
Acc16I TGCGCA 1 cut(s) 815
Acc65I GGTACC 1 cut(s) 1311
AccB1I GGYRCC 1 cut(s) 1311
AccB7I CCANNNNNTGG 2 cut(s) 876, 1841
AccI GTMKAC 1 cut(s) 2100
AccII CGCG 1 cut(s) 99
AccIII TCCGGA 2 cut(s) 299, 1943
AciI CCGC 6 cut(s) 5, 11, 97, 384, 461, 1976
AclWI GGATC 6 cut(s) 552, 581, 1766, 1791, 1804, 1955
AcsI RAATTY 4 cut(s) 135, 602, 926, 1429
AcuI CTGAAG 2 cut(s) 1370, 2097
AfaI GTAC 1 cut(s) 1313
AfiI CCNNNNNNNGG 5 cut(s) 541, 777, 778, 876, 1841
AflIII ACRYGT 1 cut(s) 1803
AjnI CCWGG 2 cut(s) 27, 980
AjuI GAANNNNNNNTTGG 2 cut(s) 1241, 1273
Alw21I GWGCWC 2 cut(s) 961, 1050
Alw26I GTCTC 2 cut(s) 8, 1919
AlwI GGATC 6 cut(s) 552, 581, 1766, 1791, 1804, 1955
AlwNI CAGNNNCTG 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 821
Aor13HI TCCGGA 2 cut(s) 299, 1943
AoxI GGCC 5 cut(s) 30, 321, 1378, 1869, 2005
ApeKI GCWGC 7 cut(s) 502, 811, 848, 1713, 1912, 1915, 2030
ApoI RAATTY 4 cut(s) 135, 602, 926, 1429
Asp718I GGTACC 1 cut(s) 1311
AspLEI GCGC 2 cut(s) 816, 1449
AspS9I GGNCC 7 cut(s) 297, 580, 1336, 1378, 1869, 1903, 2005
AsuHPI GGTGA 7 cut(s) 283, 1162, 1343, 1418, 1732, 1739, 2048
AvaI CYCGRG 1 cut(s) 821
AvaII GGWCC 4 cut(s) 297, 580, 1336, 1903
BaeGI GKGCMC 1 cut(s) 287
BamHI GGATCC 1 cut(s) 1796
BanI GGYRCC 1 cut(s) 1311
BanII GRGCYC 2 cut(s) 961, 1050
BauI CACGAG 2 cut(s) 773, 1116
BbsI GAAGAC 3 cut(s) 644, 1278, 1823
Bbv12I GWGCWC 2 cut(s) 961, 1050
BbvCI CCTCAGC 1 cut(s) 2009
BbvI GCAGC 7 cut(s) 514, 798, 835, 1700, 1899, 1902, 2042
BccI CCATC 3 cut(s) 239, 1523, 2083
BcgI CGANNNNNNTGC 2 cut(s) 1484, 1518
BciT130I CCWGG 2 cut(s) 29, 982
BciVI GTATCC 1 cut(s) 1457
BclI TGATCA 2 cut(s) 57, 1152
BcoDI GTCTC 2 cut(s) 8, 1919
BfaI CTAG 3 cut(s) 483, 506, 1809
BfmI CTRYAG 1 cut(s) 1506
BfoI RGCGCY 1 cut(s) 1450
BfuI GTATCC 1 cut(s) 1457
BglI GCCNNNNNGGC 1 cut(s) 1295
BisI GCNGC 8 cut(s) 503, 812, 849, 1714, 1913, 1916, 1977, 2031
BlsI GCNGC 8 cut(s) 504, 813, 850, 1715, 1914, 1917, 1978, 2032
Bme1390I CCNGG 2 cut(s) 29, 982
Bme18I GGWCC 4 cut(s) 297, 580, 1336, 1903
BmeT110I CYCGRG 1 cut(s) 821
BmgT120I GGNCC 7 cut(s) 297, 580, 1336, 1378, 1869, 1903, 2005
BmiI GGNNCC 5 cut(s) 304, 469, 517, 1313, 1798
BmrFI CCNGG 2 cut(s) 29, 982
BmrI ACTGGG 1 cut(s) 886
BmsI GCATC 5 cut(s) 112, 163, 1113, 1477, 2032
BmuI ACTGGG 1 cut(s) 886
BpiI GAAGAC 3 cut(s) 644, 1278, 1823
BplI GAGNNNNNCTC 2 cut(s) 1226, 1258
BpmI CTGGAG 1 cut(s) 581
Bpu10I CCTNAGC 2 cut(s) 1770, 2009
BpuEI CTTGAG 2 cut(s) 521, 1436
Bsa29I ATCGAT 1 cut(s) 1494
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 306, 822, 834
BsaWI WCCGGW 4 cut(s) 181, 299, 1308, 1943
BsaXI ACNNNNNCTCC 4 cut(s) 212, 242, 533, 563
Bsc4I CCNNNNNNNGG 5 cut(s) 541, 777, 778, 876, 1841
Bse1I ACTGG 2 cut(s) 881, 1408
BseAI TCCGGA 2 cut(s) 299, 1943
BseBI CCWGG 2 cut(s) 29, 982
BseCI ATCGAT 1 cut(s) 1494
BseDI CCNNGG 3 cut(s) 306, 822, 834
BseGI GGATG 7 cut(s) 13, 844, 1050, 1492, 1963, 1998, 2029
BseLI CCNNNNNNNGG 5 cut(s) 541, 777, 778, 876, 1841
BseMII CTCAG 7 cut(s) 41, 390, 636, 1575, 1784, 1792, 2023
BseNI ACTGG 2 cut(s) 881, 1408
BseRI GAGGAG 2 cut(s) 1622, 1868
BseSI GKGCMC 1 cut(s) 287
BseXI GCAGC 7 cut(s) 514, 798, 835, 1700, 1899, 1902, 2042
BseYI CCCAGC 1 cut(s) 2002
Bsh1236I CGCG 1 cut(s) 99
Bsh1285I CGRYCG 1 cut(s) 1494
BshFI GGCC 5 cut(s) 32, 323, 1380, 1871, 2007
BshNI GGYRCC 1 cut(s) 1311
BshVI ATCGAT 1 cut(s) 1494
BsiEI CGRYCG 1 cut(s) 1494
BsiHKAI GWGCWC 2 cut(s) 961, 1050
BsiHKCI CYCGRG 1 cut(s) 821
BsiSI CCGG 4 cut(s) 182, 300, 1309, 1944
BslFI GGGAC 2 cut(s) 932, 1796
BslI CCNNNNNNNGG 5 cut(s) 541, 777, 778, 876, 1841
BsmAI GTCTC 2 cut(s) 8, 1919
BsmFI GGGAC 2 cut(s) 932, 1796
BsmI GAATGC 2 cut(s) 851, 1238
BsnI GGCC 5 cut(s) 32, 323, 1380, 1871, 2007
Bso31I GGTCTC 1 cut(s) 8
BsoBI CYCGRG 1 cut(s) 821
Bsp1286I GDGCHC 3 cut(s) 287, 961, 1050
Bsp13I TCCGGA 2 cut(s) 299, 1943
BspACI CCGC 6 cut(s) 5, 11, 97, 384, 461, 1976
BspANI GGCC 5 cut(s) 32, 323, 1380, 1871, 2007
BspCNI CTCAG 7 cut(s) 42, 391, 637, 1576, 1783, 1791, 2022
BspDI ATCGAT 1 cut(s) 1494
BspEI TCCGGA 2 cut(s) 299, 1943
BspFNI CGCG 1 cut(s) 99
BspHI TCATGA 2 cut(s) 1267, 1761
BspLI GGNNCC 5 cut(s) 304, 469, 517, 1313, 1798
BspPI GGATC 6 cut(s) 552, 581, 1766, 1791, 1804, 1955
BspQI GCTCTTC 1 cut(s) 1038
BspT107I GGYRCC 1 cut(s) 1311
BspTNI GGTCTC 1 cut(s) 8
BsrI ACTGG 2 cut(s) 881, 1408
BssECI CCNNGG 3 cut(s) 306, 822, 834
BssSI CACGAG 2 cut(s) 773, 1116
BssT1I CCWWGG 2 cut(s) 306, 834
Bst2BI CACGAG 2 cut(s) 773, 1116
Bst2UI CCWGG 2 cut(s) 29, 982
Bst4CI ACNGT 3 cut(s) 363, 478, 1040
Bst6I CTCTTC 7 cut(s) 536, 687, 1038, 1584, 1595, 1859, 2073
BstC8I GCNNGC 4 cut(s) 167, 459, 818, 2055
BstDEI CTNAG 7 cut(s) 50, 399, 645, 1584, 1770, 1778, 2009
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 7 cut(s) 13, 844, 1050, 1492, 1963, 1998, 2029
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 1450
BstHHI GCGC 2 cut(s) 816, 1449
BstMAI GTCTC 2 cut(s) 8, 1919
BstMCI CGRYCG 1 cut(s) 1494
BstMWI GCNNNNNNNGC 3 cut(s) 29, 817, 1295
BstNI CCWGG 2 cut(s) 29, 982
BstNSI RCATGY 1 cut(s) 1807
BstSCI CCNGG 2 cut(s) 27, 980
BstSFI CTRYAG 1 cut(s) 1506
BstSLI GKGCMC 1 cut(s) 287
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 7 cut(s) 514, 798, 835, 1700, 1899, 1902, 2042
BstV2I GAAGAC 3 cut(s) 644, 1278, 1823
BstX2I RGATCY 1 cut(s) 1796
BstYI RGATCY 1 cut(s) 1796
Bsu15I ATCGAT 1 cut(s) 1494
BsuI GTATCC 1 cut(s) 1457
BsuRI GGCC 5 cut(s) 32, 323, 1380, 1871, 2007
BsuTUI ATCGAT 1 cut(s) 1494
BtsCI GGATG 7 cut(s) 13, 844, 1050, 1492, 1963, 1998, 2029
BtsI GCAGTG 1 cut(s) 1428
BtsIMutI CAGTG 3 cut(s) 474, 1103, 1428
Cac8I GCNNGC 4 cut(s) 167, 459, 818, 2055
CaiI CAGNNNCTG 1 cut(s) 49
CciI TCATGA 2 cut(s) 1267, 1761
CfoI GCGC 2 cut(s) 816, 1449
Cfr13I GGNCC 7 cut(s) 297, 580, 1336, 1378, 1869, 1903, 2005
ClaI ATCGAT 1 cut(s) 1494
Csp6I GTAC 1 cut(s) 1312
CviAII CATG 8 cut(s) 253, 352, 1268, 1480, 1762, 1792, 1804, 1849
CviQI GTAC 1 cut(s) 1312
DdeI CTNAG 7 cut(s) 50, 399, 645, 1584, 1770, 1778, 2009
DrdI GACNNNNNNGTC 1 cut(s) 1895
DseDI GACNNNNNNGTC 1 cut(s) 1895
Eam1104I CTCTTC 7 cut(s) 536, 687, 1038, 1584, 1595, 1859, 2073
EarI CTCTTC 7 cut(s) 536, 687, 1038, 1584, 1595, 1859, 2073
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 959, 1048
Eco130I CCWWGG 2 cut(s) 306, 834
Eco147I AGGCCT 1 cut(s) 323
Eco24I GRGCYC 2 cut(s) 961, 1050
Eco31I GGTCTC 1 cut(s) 8
Eco47I GGWCC 4 cut(s) 297, 580, 1336, 1903
Eco53kI GAGCTC 2 cut(s) 959, 1048
Eco57I CTGAAG 2 cut(s) 1370, 2097
Eco88I CYCGRG 1 cut(s) 821
EcoICRI GAGCTC 2 cut(s) 959, 1048
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoO109I RGGNCCY 3 cut(s) 580, 1336, 1903
EcoRI GAATTC 3 cut(s) 602, 926, 1429
EcoRII CCWGG 2 cut(s) 27, 980
EcoT14I CCWWGG 2 cut(s) 306, 834
EcoT38I GRGCYC 2 cut(s) 961, 1050
ErhI CCWWGG 2 cut(s) 306, 834
FaeI CATG 8 cut(s) 256, 355, 1271, 1483, 1765, 1795, 1807, 1852
FalI AAGNNNNNCTT 4 cut(s) 906, 938, 1607, 1639
FaqI GGGAC 2 cut(s) 932, 1796
FatI CATG 8 cut(s) 252, 351, 1267, 1479, 1761, 1791, 1803, 1848
FauI CCCGC 1 cut(s) 454
FbaI TGATCA 2 cut(s) 57, 1152
FblI GTMKAC 1 cut(s) 2100
Fnu4HI GCNGC 8 cut(s) 503, 812, 849, 1714, 1913, 1916, 1977, 2031
FokI GGATG 7 cut(s) 20, 851, 1037, 1499, 1970, 2005, 2036
FriOI GRGCYC 2 cut(s) 961, 1050
Fsp4HI GCNGC 8 cut(s) 503, 812, 849, 1714, 1913, 1916, 1977, 2031
FspBI CTAG 3 cut(s) 483, 506, 1809
FspI TGCGCA 1 cut(s) 815
GlaI GCGC 2 cut(s) 815, 1448
GluI GCNGC 8 cut(s) 503, 812, 849, 1714, 1913, 1916, 1977, 2031
GsaI CCCAGC 1 cut(s) 2006
GsuI CTGGAG 1 cut(s) 581
HaeII RGCGCY 1 cut(s) 1450
HaeIII GGCC 5 cut(s) 32, 323, 1380, 1871, 2007
HapII CCGG 4 cut(s) 182, 300, 1309, 1944
HhaI GCGC 2 cut(s) 816, 1449
Hin1II CATG 8 cut(s) 256, 355, 1271, 1483, 1765, 1795, 1807, 1852
Hin6I GCGC 2 cut(s) 814, 1447
HinP1I GCGC 2 cut(s) 814, 1447
HincII GTYRAC 3 cut(s) 217, 718, 2101
HindII GTYRAC 3 cut(s) 217, 718, 2101
HindIII AAGCTT 2 cut(s) 167, 1568
HpaI GTTAAC 1 cut(s) 718
HpaII CCGG 4 cut(s) 182, 300, 1309, 1944
HphI GGTGA 7 cut(s) 283, 1162, 1343, 1418, 1732, 1739, 2048
Hpy166II GTNNAC 6 cut(s) 217, 274, 493, 718, 1087, 2101
Hpy188I TCNGA 7 cut(s) 51, 57, 121, 343, 601, 1093, 1690
Hpy8I GTNNAC 6 cut(s) 217, 274, 493, 718, 1087, 2101
HpyCH4III ACNGT 3 cut(s) 363, 478, 1040
HpyCH4V TGCA 6 cut(s) 293, 328, 851, 1179, 1638, 2045
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 817, 1295
HpyF3I CTNAG 7 cut(s) 50, 399, 645, 1584, 1770, 1778, 2009
Hsp92II CATG 8 cut(s) 256, 355, 1271, 1483, 1765, 1795, 1807, 1852
HspAI GCGC 2 cut(s) 814, 1447
Kpn2I TCCGGA 2 cut(s) 299, 1943
KpnI GGTACC 1 cut(s) 1315
Ksp22I TGATCA 2 cut(s) 57, 1152
KspAI GTTAAC 1 cut(s) 718
LguI GCTCTTC 1 cut(s) 1038
LmnI GCTCC 4 cut(s) 184, 781, 964, 1444
Lsp1109I GCAGC 7 cut(s) 514, 798, 835, 1700, 1899, 1902, 2042
LweI GCATC 5 cut(s) 112, 163, 1113, 1477, 2032
MaeI CTAG 3 cut(s) 483, 506, 1809
MaeIII GTNAC 4 cut(s) 412, 478, 1360, 1720
MflI RGATCY 1 cut(s) 1796
MhlI GDGCHC 3 cut(s) 287, 961, 1050
MluCI AATT 8 cut(s) 112, 135, 602, 828, 926, 1292, 1429, 1695
MlyI GAGTC 2 cut(s) 61, 1082
MmeI TCCRAC 1 cut(s) 531
MroI TCCGGA 2 cut(s) 299, 1943
MseI TTAA 3 cut(s) 717, 1002, 1074
MslI CAYNNNNRTG 3 cut(s) 251, 429, 1184
MspA1I CMGCKG 1 cut(s) 2033
MspI CCGG 4 cut(s) 182, 300, 1309, 1944
MspR9I CCNGG 2 cut(s) 29, 982
Mva1269I GAATGC 2 cut(s) 851, 1238
MvaI CCWGG 2 cut(s) 29, 982
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 3 cut(s) 29, 817, 1295
NlaIII CATG 8 cut(s) 256, 355, 1271, 1483, 1765, 1795, 1807, 1852
NlaIV GGNNCC 5 cut(s) 304, 469, 517, 1313, 1798
NmeAIII GCCGAG 1 cut(s) 58
NmuCI GTSAC 3 cut(s) 412, 478, 1720
NsbI TGCGCA 1 cut(s) 815
NspI RCATGY 1 cut(s) 1807
PagI TCATGA 2 cut(s) 1267, 1761
PceI AGGCCT 1 cut(s) 323
PciI ACATGT 1 cut(s) 1803
PciSI GCTCTTC 1 cut(s) 1038
PcsI WCGNNNNNNNCGW 1 cut(s) 1089
PctI GAATGC 2 cut(s) 851, 1238
PfeI GAWTC 8 cut(s) 141, 452, 737, 1630, 1703, 1845, 1947, 1983
PflMI CCANNNNNTGG 2 cut(s) 876, 1841
PkrI GCNGC 8 cut(s) 504, 813, 850, 1715, 1914, 1917, 1978, 2032
Ple19I CGATCG 1 cut(s) 1494
PleI GAGTC 2 cut(s) 60, 1082
PpsI GAGTC 2 cut(s) 60, 1082
PpuMI RGGWCCY 3 cut(s) 580, 1336, 1903
PscI ACATGT 1 cut(s) 1803
Psp124BI GAGCTC 2 cut(s) 961, 1050
Psp5II RGGWCCY 3 cut(s) 580, 1336, 1903
Psp6I CCWGG 2 cut(s) 27, 980
PspFI CCCAGC 1 cut(s) 2002
PspGI CCWGG 2 cut(s) 27, 980
PspN4I GGNNCC 5 cut(s) 304, 469, 517, 1313, 1798
PspPI GGNCC 7 cut(s) 297, 580, 1336, 1378, 1869, 1903, 2005
PspPPI RGGWCCY 3 cut(s) 580, 1336, 1903
PstNI CAGNNNCTG 1 cut(s) 49
PsuI RGATCY 1 cut(s) 1796
PvuI CGATCG 1 cut(s) 1494
PvuII CAGCTG 1 cut(s) 2033
RsaI GTAC 1 cut(s) 1313
RsaNI GTAC 1 cut(s) 1312
RseI CAYNNNNRTG 3 cut(s) 251, 429, 1184
SacI GAGCTC 2 cut(s) 961, 1050
SalI GTCGAC 1 cut(s) 2099
SapI GCTCTTC 1 cut(s) 1038
SaqAI TTAA 3 cut(s) 717, 1002, 1074
SatI GCNGC 8 cut(s) 503, 812, 849, 1714, 1913, 1916, 1977, 2031
Sau96I GGNCC 7 cut(s) 297, 580, 1336, 1378, 1869, 1903, 2005
SchI GAGTC 2 cut(s) 61, 1082
ScrFI CCNGG 2 cut(s) 29, 982
SduI GDGCHC 3 cut(s) 287, 961, 1050
SfaNI GCATC 5 cut(s) 112, 163, 1113, 1477, 2032
SfcI CTRYAG 1 cut(s) 1506
SinI GGWCC 4 cut(s) 297, 580, 1336, 1903
SmiMI CAYNNNNRTG 3 cut(s) 251, 429, 1184
SmlI CTYRAG 2 cut(s) 536, 1451
SmoI CTYRAG 2 cut(s) 536, 1451
Sse9I AATT 8 cut(s) 112, 135, 602, 828, 926, 1292, 1429, 1695
SseBI AGGCCT 1 cut(s) 323
SsiI CCGC 6 cut(s) 5, 11, 97, 384, 461, 1976
SspMI CTAG 3 cut(s) 483, 506, 1809
SstI GAGCTC 2 cut(s) 961, 1050
StuI AGGCCT 1 cut(s) 323
StyD4I CCNGG 2 cut(s) 27, 980
StyI CCWWGG 2 cut(s) 306, 834
TaaI ACNGT 3 cut(s) 363, 478, 1040
TaqI TCGA 6 cut(s) 139, 144, 966, 1494, 1923, 2100
TasI AATT 8 cut(s) 112, 135, 602, 828, 926, 1292, 1429, 1695
TauI GCSGC 1 cut(s) 1979
TfiI GAWTC 8 cut(s) 141, 452, 737, 1630, 1703, 1845, 1947, 1983
Tru1I TTAA 3 cut(s) 717, 1002, 1074
Tru9I TTAA 3 cut(s) 717, 1002, 1074
TscAI CASTG 3 cut(s) 481, 1110, 1428
TseFI GTSAC 3 cut(s) 412, 478, 1720
TseI GCWGC 7 cut(s) 502, 811, 848, 1713, 1912, 1915, 2030
Tsp45I GTSAC 3 cut(s) 412, 478, 1720
TspGWI ACGGA 4 cut(s) 170, 510, 647, 951
TspRI CASTG 3 cut(s) 481, 1110, 1428
Van91I CCANNNNNTGG 2 cut(s) 876, 1841
VpaK11BI GGWCC 4 cut(s) 297, 580, 1336, 1903
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 4 cut(s) 135, 602, 926, 1429
XceI RCATGY 1 cut(s) 1807
XcmI CCANNNNNNNNNTGG 2 cut(s) 265, 313
XmiI GTMKAC 1 cut(s) 2100
XspI CTAG 3 cut(s) 483, 506, 1809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.