Splicing factor 1 helix-hairpin domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 628 |
| AciI | CCGC | 1 cut(s) | 626 |
| AcoI | YGGCCR | 1 cut(s) | 27 |
| AcsI | RAATTY | 2 cut(s) | 20, 208 |
| AfaI | GTAC | 4 cut(s) | 14, 148, 215, 224 |
| AgsI | TTSAA | 4 cut(s) | 181, 362, 556, 670 |
| AjnI | CCWGG | 1 cut(s) | 436 |
| AluBI | AGCT | 4 cut(s) | 449, 571, 688, 743 |
| AluI | AGCT | 4 cut(s) | 449, 571, 688, 743 |
| Alw21I | GWGCWC | 1 cut(s) | 690 |
| Alw26I | GTCTC | 1 cut(s) | 438 |
| AoxI | GGCC | 1 cut(s) | 27 |
| ApeKI | GCWGC | 1 cut(s) | 353 |
| ApoI | RAATTY | 2 cut(s) | 20, 208 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 61, 93 |
| AspS9I | GGNCC | 3 cut(s) | 307, 440, 493 |
| AsuC2I | CCSGG | 1 cut(s) | 311 |
| AsuHPI | GGTGA | 1 cut(s) | 453 |
| AvaII | GGWCC | 3 cut(s) | 307, 440, 493 |
| BanII | GRGCYC | 2 cut(s) | 170, 690 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 96, 128 |
| BbsI | GAAGAC | 1 cut(s) | 677 |
| Bbv12I | GWGCWC | 1 cut(s) | 690 |
| BbvI | GCAGC | 1 cut(s) | 340 |
| BceAI | ACGGC | 1 cut(s) | 87 |
| BciT130I | CCWGG | 1 cut(s) | 438 |
| BciVI | GTATCC | 1 cut(s) | 495 |
| BcnI | CCSGG | 1 cut(s) | 311 |
| BcoDI | GTCTC | 1 cut(s) | 438 |
| BfaI | CTAG | 1 cut(s) | 158 |
| BfmI | CTRYAG | 1 cut(s) | 578 |
| BfuI | GTATCC | 1 cut(s) | 495 |
| BisI | GCNGC | 2 cut(s) | 354, 626 |
| BlsI | GCNGC | 2 cut(s) | 355, 627 |
| BmcAI | AGTACT | 1 cut(s) | 224 |
| Bme1390I | CCNGG | 2 cut(s) | 311, 438 |
| Bme18I | GGWCC | 3 cut(s) | 307, 440, 493 |
| BmgT120I | GGNCC | 3 cut(s) | 307, 440, 493 |
| BmrFI | CCNGG | 2 cut(s) | 311, 438 |
| BpiI | GAAGAC | 1 cut(s) | 677 |
| BpuMI | CCSGG | 1 cut(s) | 311 |
| BsaJI | CCNNGG | 3 cut(s) | 116, 189, 586 |
| Bse1I | ACTGG | 2 cut(s) | 322, 495 |
| BseBI | CCWGG | 1 cut(s) | 438 |
| BseDI | CCNNGG | 3 cut(s) | 116, 189, 586 |
| BseGI | GGATG | 2 cut(s) | 10, 607 |
| BseNI | ACTGG | 2 cut(s) | 322, 495 |
| BseXI | GCAGC | 1 cut(s) | 340 |
| BseYI | CCCAGC | 1 cut(s) | 131 |
| BshFI | GGCC | 1 cut(s) | 29 |
| BsiHKAI | GWGCWC | 1 cut(s) | 690 |
| BsiSI | CCGG | 1 cut(s) | 310 |
| BslFI | GGGAC | 1 cut(s) | 55 |
| BsmAI | GTCTC | 1 cut(s) | 438 |
| BsmFI | GGGAC | 1 cut(s) | 55 |
| BsmI | GAATGC | 1 cut(s) | 142 |
| BsnI | GGCC | 1 cut(s) | 29 |
| Bsp1286I | GDGCHC | 2 cut(s) | 170, 690 |
| Bsp1407I | TGTACA | 1 cut(s) | 213 |
| BspACI | CCGC | 1 cut(s) | 626 |
| BspANI | GGCC | 1 cut(s) | 29 |
| BsrBI | CCGCTC | 1 cut(s) | 628 |
| BsrGI | TGTACA | 1 cut(s) | 213 |
| BsrI | ACTGG | 2 cut(s) | 322, 495 |
| BssECI | CCNNGG | 3 cut(s) | 116, 189, 586 |
| BssT1I | CCWWGG | 3 cut(s) | 116, 189, 586 |
| Bst2UI | CCWGG | 1 cut(s) | 438 |
| Bst4CI | ACNGT | 3 cut(s) | 563, 676, 703 |
| BstAUI | TGTACA | 1 cut(s) | 213 |
| BstC8I | GCNNGC | 1 cut(s) | 385 |
| BstF5I | GGATG | 2 cut(s) | 10, 607 |
| BstMAI | GTCTC | 1 cut(s) | 438 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 80, 137, 694 |
| BstNI | CCWGG | 1 cut(s) | 438 |
| BstNSI | RCATGY | 1 cut(s) | 96 |
| BstSCI | CCNGG | 2 cut(s) | 309, 436 |
| BstSFI | CTRYAG | 1 cut(s) | 578 |
| BstV1I | GCAGC | 1 cut(s) | 340 |
| BstV2I | GAAGAC | 1 cut(s) | 677 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 476 |
| BsuI | GTATCC | 1 cut(s) | 495 |
| BsuRI | GGCC | 1 cut(s) | 29 |
| BtsCI | GGATG | 2 cut(s) | 10, 607 |
| BtsI | GCAGTG | 1 cut(s) | 732 |
| BtsIMutI | CAGTG | 2 cut(s) | 488, 732 |
| Cac8I | GCNNGC | 1 cut(s) | 385 |
| Cfr13I | GGNCC | 3 cut(s) | 307, 440, 493 |
| Csp6I | GTAC | 4 cut(s) | 13, 147, 214, 223 |
| CviAII | CATG | 3 cut(s) | 93, 270, 388 |
| CviQI | GTAC | 4 cut(s) | 13, 147, 214, 223 |
| EaeI | YGGCCR | 1 cut(s) | 27 |
| Ecl136II | GAGCTC | 1 cut(s) | 688 |
| Eco130I | CCWWGG | 3 cut(s) | 116, 189, 586 |
| Eco24I | GRGCYC | 2 cut(s) | 170, 690 |
| Eco47I | GGWCC | 3 cut(s) | 307, 440, 493 |
| Eco53kI | GAGCTC | 1 cut(s) | 688 |
| EcoICRI | GAGCTC | 1 cut(s) | 688 |
| EcoRII | CCWGG | 1 cut(s) | 436 |
| EcoT14I | CCWWGG | 3 cut(s) | 116, 189, 586 |
| EcoT38I | GRGCYC | 2 cut(s) | 170, 690 |
| ErhI | CCWWGG | 3 cut(s) | 116, 189, 586 |
| FaeI | CATG | 3 cut(s) | 96, 273, 391 |
| FaiI | YATR | 8 cut(s) | 94, 198, 271, 336, 351, 389, 431, 534 |
| FaqI | GGGAC | 1 cut(s) | 55 |
| FatI | CATG | 3 cut(s) | 92, 269, 387 |
| Fnu4HI | GCNGC | 2 cut(s) | 354, 626 |
| FokI | GGATG | 2 cut(s) | 17, 614 |
| FriOI | GRGCYC | 2 cut(s) | 170, 690 |
| Fsp4HI | GCNGC | 2 cut(s) | 354, 626 |
| FspBI | CTAG | 1 cut(s) | 158 |
| GluI | GCNGC | 2 cut(s) | 354, 626 |
| GsaI | CCCAGC | 1 cut(s) | 135 |
| HaeIII | GGCC | 1 cut(s) | 29 |
| HapII | CCGG | 1 cut(s) | 310 |
| Hin1II | CATG | 3 cut(s) | 96, 273, 391 |
| HincII | GTYRAC | 1 cut(s) | 124 |
| HindII | GTYRAC | 1 cut(s) | 124 |
| HindIII | AAGCTT | 1 cut(s) | 741 |
| HinfI | GANTC | 4 cut(s) | 32, 53, 591, 611 |
| HpaI | GTTAAC | 1 cut(s) | 124 |
| HpaII | CCGG | 1 cut(s) | 310 |
| HphI | GGTGA | 1 cut(s) | 453 |
| Hpy166II | GTNNAC | 3 cut(s) | 124, 549, 610 |
| Hpy188I | TCNGA | 4 cut(s) | 52, 401, 616, 680 |
| Hpy188III | TCNNGA | 3 cut(s) | 239, 538, 595 |
| Hpy8I | GTNNAC | 3 cut(s) | 124, 549, 610 |
| HpyAV | CCTTC | 2 cut(s) | 298, 592 |
| HpyCH4III | ACNGT | 3 cut(s) | 563, 676, 703 |
| HpyCH4V | TGCA | 3 cut(s) | 96, 294, 383 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 80, 137, 694 |
| Hsp92II | CATG | 3 cut(s) | 96, 273, 391 |
| KspAI | GTTAAC | 1 cut(s) | 124 |
| Lsp1109I | GCAGC | 1 cut(s) | 340 |
| MaeI | CTAG | 1 cut(s) | 158 |
| MaeIII | GTNAC | 1 cut(s) | 459 |
| MbiI | CCGCTC | 1 cut(s) | 628 |
| MboII | GAAGA | 4 cut(s) | 104, 523, 526, 682 |
| MfeI | CAATTG | 1 cut(s) | 691 |
| MhlI | GDGCHC | 2 cut(s) | 170, 690 |
| MluCI | AATT | 5 cut(s) | 20, 97, 208, 529, 691 |
| MlyI | GAGTC | 2 cut(s) | 41, 605 |
| MnlI | CCTC | 6 cut(s) | 195, 339, 432, 506, 534, 723 |
| MseI | TTAA | 1 cut(s) | 123 |
| MspI | CCGG | 1 cut(s) | 310 |
| MspR9I | CCNGG | 2 cut(s) | 311, 438 |
| MunI | CAATTG | 1 cut(s) | 691 |
| Mva1269I | GAATGC | 1 cut(s) | 142 |
| MvaI | CCWGG | 1 cut(s) | 438 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 80, 137, 694 |
| NciI | CCSGG | 1 cut(s) | 311 |
| NlaIII | CATG | 3 cut(s) | 96, 273, 391 |
| NmeAIII | GCCGAG | 1 cut(s) | 55 |
| NmuCI | GTSAC | 1 cut(s) | 459 |
| NspI | RCATGY | 1 cut(s) | 96 |
| PctI | GAATGC | 1 cut(s) | 142 |
| PfeI | GAWTC | 2 cut(s) | 53, 591 |
| PflFI | GACNNNGTC | 1 cut(s) | 46 |
| PfoI | TCCNGGA | 1 cut(s) | 436 |
| PkrI | GCNGC | 2 cut(s) | 355, 627 |
| PleI | GAGTC | 2 cut(s) | 40, 605 |
| PpsI | GAGTC | 2 cut(s) | 40, 605 |
| Psp124BI | GAGCTC | 1 cut(s) | 690 |
| Psp6I | CCWGG | 1 cut(s) | 436 |
| PspFI | CCCAGC | 1 cut(s) | 131 |
| PspGI | CCWGG | 1 cut(s) | 436 |
| PspPI | GGNCC | 3 cut(s) | 307, 440, 493 |
| PsyI | GACNNNGTC | 1 cut(s) | 46 |
| RsaI | GTAC | 4 cut(s) | 14, 148, 215, 224 |
| RsaNI | GTAC | 4 cut(s) | 13, 147, 214, 223 |
| SacI | GAGCTC | 1 cut(s) | 690 |
| SaqAI | TTAA | 1 cut(s) | 123 |
| SatI | GCNGC | 2 cut(s) | 354, 626 |
| Sau96I | GGNCC | 3 cut(s) | 307, 440, 493 |
| ScaI | AGTACT | 1 cut(s) | 224 |
| SchI | GAGTC | 2 cut(s) | 41, 605 |
| ScrFI | CCNGG | 2 cut(s) | 311, 438 |
| SduI | GDGCHC | 2 cut(s) | 170, 690 |
| SfcI | CTRYAG | 1 cut(s) | 578 |
| SinI | GGWCC | 3 cut(s) | 307, 440, 493 |
| Sse9I | AATT | 5 cut(s) | 20, 97, 208, 529, 691 |
| SsiI | CCGC | 1 cut(s) | 626 |
| SspI | AATATT | 1 cut(s) | 283 |
| SspMI | CTAG | 1 cut(s) | 158 |
| SstI | GAGCTC | 1 cut(s) | 690 |
| StyD4I | CCNGG | 2 cut(s) | 309, 436 |
| StyI | CCWWGG | 3 cut(s) | 116, 189, 586 |
| TaaI | ACNGT | 3 cut(s) | 563, 676, 703 |
| TaqI | TCGA | 2 cut(s) | 219, 452 |
| TasI | AATT | 5 cut(s) | 20, 97, 208, 529, 691 |
| TatI | WGTACW | 3 cut(s) | 146, 213, 222 |
| TauI | GCSGC | 1 cut(s) | 628 |
| TfiI | GAWTC | 2 cut(s) | 53, 591 |
| Tru1I | TTAA | 1 cut(s) | 123 |
| Tru9I | TTAA | 1 cut(s) | 123 |
| TscAI | CASTG | 2 cut(s) | 495, 732 |
| TseFI | GTSAC | 1 cut(s) | 459 |
| TseI | GCWGC | 1 cut(s) | 353 |
| Tsp45I | GTSAC | 1 cut(s) | 459 |
| TspDTI | ATGAA | 3 cut(s) | 258, 317, 533 |
| TspRI | CASTG | 2 cut(s) | 495, 732 |
| Tth111I | GACNNNGTC | 1 cut(s) | 46 |
| VpaK11BI | GGWCC | 3 cut(s) | 307, 440, 493 |
| XapI | RAATTY | 2 cut(s) | 20, 208 |
| XceI | RCATGY | 1 cut(s) | 96 |
| XspI | CTAG | 1 cut(s) | 158 |
| ZrmI | AGTACT | 1 cut(s) | 224 |