Heat shock protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 773 |
| Acc65I | GGTACC | 1 cut(s) | 189 |
| AccB1I | GGYRCC | 1 cut(s) | 189 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 719 |
| AccI | GTMKAC | 1 cut(s) | 978 |
| AccIII | TCCGGA | 1 cut(s) | 821 |
| AclWI | GGATC | 4 cut(s) | 644, 669, 682, 833 |
| AcsI | RAATTY | 1 cut(s) | 307 |
| AcuI | CTGAAG | 2 cut(s) | 248, 975 |
| AfaI | GTAC | 1 cut(s) | 191 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 719 |
| AflIII | ACRYGT | 1 cut(s) | 681 |
| AgsI | TTSAA | 7 cut(s) | 8, 25, 70, 133, 397, 445, 877 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 119, 151 |
| AluBI | AGCT | 5 cut(s) | 448, 755, 831, 880, 911 |
| AluI | AGCT | 5 cut(s) | 448, 755, 831, 880, 911 |
| Alw26I | GTCTC | 1 cut(s) | 797 |
| AlwI | GGATC | 4 cut(s) | 644, 669, 682, 833 |
| Aor13HI | TCCGGA | 1 cut(s) | 821 |
| AoxI | GGCC | 3 cut(s) | 256, 747, 883 |
| ApeKI | GCWGC | 6 cut(s) | 591, 790, 793, 854, 908, 950 |
| ApoI | RAATTY | 1 cut(s) | 307 |
| Asp718I | GGTACC | 1 cut(s) | 189 |
| AspLEI | GCGC | 1 cut(s) | 327 |
| AspS9I | GGNCC | 5 cut(s) | 214, 256, 747, 781, 883 |
| AsuHPI | GGTGA | 6 cut(s) | 40, 221, 296, 610, 617, 926 |
| AvaII | GGWCC | 2 cut(s) | 214, 781 |
| BamHI | GGATCC | 1 cut(s) | 674 |
| BanI | GGYRCC | 1 cut(s) | 189 |
| BbsI | GAAGAC | 2 cut(s) | 156, 701 |
| BbvCI | CCTCAGC | 1 cut(s) | 887 |
| BbvI | GCAGC | 6 cut(s) | 578, 777, 780, 866, 920, 937 |
| BccI | CCATC | 2 cut(s) | 401, 961 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 362, 396 |
| BclI | TGATCA | 1 cut(s) | 30 |
| BcoDI | GTCTC | 1 cut(s) | 797 |
| BfaI | CTAG | 1 cut(s) | 687 |
| BfmI | CTRYAG | 1 cut(s) | 384 |
| BfoI | RGCGCY | 1 cut(s) | 328 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 173 |
| BisI | GCNGC | 6 cut(s) | 592, 791, 794, 855, 909, 951 |
| BlsI | GCNGC | 6 cut(s) | 593, 792, 795, 856, 910, 952 |
| Bme18I | GGWCC | 2 cut(s) | 214, 781 |
| BmgT120I | GGNCC | 5 cut(s) | 214, 256, 747, 781, 883 |
| BmiI | GGNNCC | 2 cut(s) | 191, 676 |
| BmsI | GCATC | 3 cut(s) | 355, 910, 937 |
| BpiI | GAAGAC | 2 cut(s) | 156, 701 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 104, 136 |
| Bpu10I | CCTNAGC | 2 cut(s) | 648, 887 |
| BpuEI | CTTGAG | 1 cut(s) | 314 |
| Bsa29I | ATCGAT | 1 cut(s) | 372 |
| BsaWI | WCCGGW | 2 cut(s) | 186, 821 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 719 |
| Bse1I | ACTGG | 1 cut(s) | 286 |
| BseAI | TCCGGA | 1 cut(s) | 821 |
| BseCI | ATCGAT | 1 cut(s) | 372 |
| BseGI | GGATG | 4 cut(s) | 370, 841, 876, 907 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 719 |
| BseMII | CTCAG | 4 cut(s) | 453, 662, 670, 901 |
| BseNI | ACTGG | 1 cut(s) | 286 |
| BseRI | GAGGAG | 2 cut(s) | 500, 746 |
| BseXI | GCAGC | 6 cut(s) | 578, 777, 780, 866, 920, 937 |
| BseYI | CCCAGC | 1 cut(s) | 880 |
| BshFI | GGCC | 3 cut(s) | 258, 749, 885 |
| BshNI | GGYRCC | 1 cut(s) | 189 |
| BshVI | ATCGAT | 1 cut(s) | 372 |
| BsiSI | CCGG | 2 cut(s) | 187, 822 |
| BslFI | GGGAC | 1 cut(s) | 674 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 719 |
| BsmAI | GTCTC | 1 cut(s) | 797 |
| BsmFI | GGGAC | 1 cut(s) | 674 |
| BsmI | GAATGC | 1 cut(s) | 116 |
| BsnI | GGCC | 3 cut(s) | 258, 749, 885 |
| Bsp13I | TCCGGA | 1 cut(s) | 821 |
| Bsp143I | GATC | 4 cut(s) | 30, 636, 674, 838 |
| BspANI | GGCC | 3 cut(s) | 258, 749, 885 |
| BspCNI | CTCAG | 4 cut(s) | 454, 661, 669, 900 |
| BspDI | ATCGAT | 1 cut(s) | 372 |
| BspEI | TCCGGA | 1 cut(s) | 821 |
| BspHI | TCATGA | 2 cut(s) | 145, 639 |
| BspLI | GGNNCC | 2 cut(s) | 191, 676 |
| BspPI | GGATC | 4 cut(s) | 644, 669, 682, 833 |
| BspT107I | GGYRCC | 1 cut(s) | 189 |
| BsrI | ACTGG | 1 cut(s) | 286 |
| BssMI | GATC | 4 cut(s) | 30, 636, 674, 838 |
| Bst6I | CTCTTC | 4 cut(s) | 462, 473, 737, 951 |
| BstC8I | GCNNGC | 1 cut(s) | 933 |
| BstDEI | CTNAG | 5 cut(s) | 102, 462, 648, 656, 887 |
| BstF5I | GGATG | 4 cut(s) | 370, 841, 876, 907 |
| BstH2I | RGCGCY | 1 cut(s) | 328 |
| BstHHI | GCGC | 1 cut(s) | 327 |
| BstKTI | GATC | 4 cut(s) | 33, 639, 677, 841 |
| BstMAI | GTCTC | 1 cut(s) | 797 |
| BstMBI | GATC | 4 cut(s) | 30, 636, 674, 838 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 173 |
| BstNSI | RCATGY | 1 cut(s) | 685 |
| BstSFI | CTRYAG | 1 cut(s) | 384 |
| BstV1I | GCAGC | 6 cut(s) | 578, 777, 780, 866, 920, 937 |
| BstV2I | GAAGAC | 2 cut(s) | 156, 701 |
| BstX2I | RGATCY | 1 cut(s) | 674 |
| BstYI | RGATCY | 1 cut(s) | 674 |
| Bsu15I | ATCGAT | 1 cut(s) | 372 |
| BsuRI | GGCC | 3 cut(s) | 258, 749, 885 |
| BsuTUI | ATCGAT | 1 cut(s) | 372 |
| BtsCI | GGATG | 4 cut(s) | 370, 841, 876, 907 |
| BtsI | GCAGTG | 1 cut(s) | 306 |
| BtsIMutI | CAGTG | 1 cut(s) | 306 |
| Cac8I | GCNNGC | 1 cut(s) | 933 |
| CciI | TCATGA | 2 cut(s) | 145, 639 |
| CfoI | GCGC | 1 cut(s) | 327 |
| Cfr13I | GGNCC | 5 cut(s) | 214, 256, 747, 781, 883 |
| ClaI | ATCGAT | 1 cut(s) | 372 |
| Csp6I | GTAC | 1 cut(s) | 190 |
| CviAII | CATG | 6 cut(s) | 146, 358, 640, 670, 682, 727 |
| CviQI | GTAC | 1 cut(s) | 190 |
| DdeI | CTNAG | 5 cut(s) | 102, 462, 648, 656, 887 |
| DpnI | GATC | 4 cut(s) | 32, 638, 676, 840 |
| DpnII | GATC | 4 cut(s) | 30, 636, 674, 838 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 773 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 773 |
| Eam1104I | CTCTTC | 4 cut(s) | 462, 473, 737, 951 |
| EarI | CTCTTC | 4 cut(s) | 462, 473, 737, 951 |
| Eco47I | GGWCC | 2 cut(s) | 214, 781 |
| Eco57I | CTGAAG | 2 cut(s) | 248, 975 |
| EcoO109I | RGGNCCY | 2 cut(s) | 214, 781 |
| EcoRI | GAATTC | 1 cut(s) | 307 |
| FaeI | CATG | 6 cut(s) | 149, 361, 643, 673, 685, 730 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 485, 517 |
| FaqI | GGGAC | 1 cut(s) | 674 |
| FatI | CATG | 6 cut(s) | 145, 357, 639, 669, 681, 726 |
| FbaI | TGATCA | 1 cut(s) | 30 |
| FblI | GTMKAC | 1 cut(s) | 978 |
| Fnu4HI | GCNGC | 6 cut(s) | 592, 791, 794, 855, 909, 951 |
| FokI | GGATG | 4 cut(s) | 377, 848, 883, 914 |
| Fsp4HI | GCNGC | 6 cut(s) | 592, 791, 794, 855, 909, 951 |
| FspBI | CTAG | 1 cut(s) | 687 |
| GlaI | GCGC | 1 cut(s) | 326 |
| GluI | GCNGC | 6 cut(s) | 592, 791, 794, 855, 909, 951 |
| GsaI | CCCAGC | 1 cut(s) | 884 |
| HaeII | RGCGCY | 1 cut(s) | 328 |
| HaeIII | GGCC | 3 cut(s) | 258, 749, 885 |
| HapII | CCGG | 2 cut(s) | 187, 822 |
| HhaI | GCGC | 1 cut(s) | 327 |
| Hin1II | CATG | 6 cut(s) | 149, 361, 643, 673, 685, 730 |
| Hin6I | GCGC | 1 cut(s) | 325 |
| HinP1I | GCGC | 1 cut(s) | 325 |
| HincII | GTYRAC | 1 cut(s) | 979 |
| HindII | GTYRAC | 1 cut(s) | 979 |
| HindIII | AAGCTT | 1 cut(s) | 446 |
| HinfI | GANTC | 5 cut(s) | 508, 581, 723, 825, 861 |
| HpaII | CCGG | 2 cut(s) | 187, 822 |
| HphI | GGTGA | 6 cut(s) | 40, 221, 296, 610, 617, 926 |
| Hpy166II | GTNNAC | 1 cut(s) | 979 |
| Hpy188I | TCNGA | 1 cut(s) | 568 |
| Hpy188III | TCNNGA | 8 cut(s) | 34, 146, 331, 640, 713, 802, 814, 822 |
| Hpy8I | GTNNAC | 1 cut(s) | 979 |
| HpyAV | CCTTC | 9 cut(s) | 19, 191, 223, 244, 289, 391, 431, 547, 637 |
| HpyCH4V | TGCA | 5 cut(s) | 57, 368, 516, 854, 923 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 173 |
| HpyF3I | CTNAG | 5 cut(s) | 102, 462, 648, 656, 887 |
| Hsp92II | CATG | 6 cut(s) | 149, 361, 643, 673, 685, 730 |
| HspAI | GCGC | 1 cut(s) | 325 |
| Kpn2I | TCCGGA | 1 cut(s) | 821 |
| KpnI | GGTACC | 1 cut(s) | 193 |
| Ksp22I | TGATCA | 1 cut(s) | 30 |
| Kzo9I | GATC | 4 cut(s) | 30, 636, 674, 838 |
| LmnI | GCTCC | 1 cut(s) | 322 |
| Lsp1109I | GCAGC | 6 cut(s) | 578, 777, 780, 866, 920, 937 |
| LweI | GCATC | 3 cut(s) | 355, 910, 937 |
| MaeI | CTAG | 1 cut(s) | 687 |
| MaeIII | GTNAC | 2 cut(s) | 238, 598 |
| MalI | GATC | 4 cut(s) | 32, 638, 676, 840 |
| MboI | GATC | 4 cut(s) | 30, 636, 674, 838 |
| MflI | RGATCY | 1 cut(s) | 674 |
| MluCI | AATT | 3 cut(s) | 170, 307, 573 |
| MroI | TCCGGA | 1 cut(s) | 821 |
| MslI | CAYNNNNRTG | 1 cut(s) | 62 |
| MspA1I | CMGCKG | 1 cut(s) | 911 |
| MspI | CCGG | 2 cut(s) | 187, 822 |
| Mva1269I | GAATGC | 1 cut(s) | 116 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 173 |
| NdeII | GATC | 4 cut(s) | 30, 636, 674, 838 |
| NlaIII | CATG | 6 cut(s) | 149, 361, 643, 673, 685, 730 |
| NlaIV | GGNNCC | 2 cut(s) | 191, 676 |
| NmuCI | GTSAC | 1 cut(s) | 598 |
| NspI | RCATGY | 1 cut(s) | 685 |
| PagI | TCATGA | 2 cut(s) | 145, 639 |
| PciI | ACATGT | 1 cut(s) | 681 |
| PctI | GAATGC | 1 cut(s) | 116 |
| PfeI | GAWTC | 5 cut(s) | 508, 581, 723, 825, 861 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 719 |
| PkrI | GCNGC | 6 cut(s) | 593, 792, 795, 856, 910, 952 |
| PpuMI | RGGWCCY | 2 cut(s) | 214, 781 |
| PscI | ACATGT | 1 cut(s) | 681 |
| Psp5II | RGGWCCY | 2 cut(s) | 214, 781 |
| PspFI | CCCAGC | 1 cut(s) | 880 |
| PspN4I | GGNNCC | 2 cut(s) | 191, 676 |
| PspPI | GGNCC | 5 cut(s) | 214, 256, 747, 781, 883 |
| PspPPI | RGGWCCY | 2 cut(s) | 214, 781 |
| PsuI | RGATCY | 1 cut(s) | 674 |
| PvuII | CAGCTG | 1 cut(s) | 911 |
| RsaI | GTAC | 1 cut(s) | 191 |
| RsaNI | GTAC | 1 cut(s) | 190 |
| RseI | CAYNNNNRTG | 1 cut(s) | 62 |
| SalI | GTCGAC | 1 cut(s) | 977 |
| SatI | GCNGC | 6 cut(s) | 592, 791, 794, 855, 909, 951 |
| Sau3AI | GATC | 4 cut(s) | 30, 636, 674, 838 |
| Sau96I | GGNCC | 5 cut(s) | 214, 256, 747, 781, 883 |
| SfaNI | GCATC | 3 cut(s) | 355, 910, 937 |
| SfcI | CTRYAG | 1 cut(s) | 384 |
| SinI | GGWCC | 2 cut(s) | 214, 781 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 62 |
| SmlI | CTYRAG | 1 cut(s) | 329 |
| SmoI | CTYRAG | 1 cut(s) | 329 |
| Sse9I | AATT | 3 cut(s) | 170, 307, 573 |
| SspMI | CTAG | 1 cut(s) | 687 |
| TaqI | TCGA | 3 cut(s) | 372, 801, 978 |
| TasI | AATT | 3 cut(s) | 170, 307, 573 |
| TfiI | GAWTC | 5 cut(s) | 508, 581, 723, 825, 861 |
| TscAI | CASTG | 1 cut(s) | 306 |
| TseFI | GTSAC | 1 cut(s) | 598 |
| TseI | GCWGC | 6 cut(s) | 591, 790, 793, 854, 908, 950 |
| Tsp45I | GTSAC | 1 cut(s) | 598 |
| TspDTI | ATGAA | 7 cut(s) | 134, 162, 263, 346, 360, 471, 656 |
| TspRI | CASTG | 1 cut(s) | 306 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 719 |
| VpaK11BI | GGWCC | 2 cut(s) | 214, 781 |
| XapI | RAATTY | 1 cut(s) | 307 |
| XceI | RCATGY | 1 cut(s) | 685 |
| XmiI | GTMKAC | 1 cut(s) | 978 |
| XspI | CTAG | 1 cut(s) | 687 |