pycom09g05820

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000545
Physical Location & Seq
Forward (+)
4334361 .. 4335095
735 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g05820.1

Sequence Viewer

Length: 735 bp
ATGATCCTCCCACAAGCGGCATGCATTCTCACTTCTCAGGACATCATCAGTATCAAATTGGGAGATATCGTCCCAGCTGATGCTCGTCTTCTCGAGGGTGATCCTTTAAAGATCGATCAATCTGCCCTCACAGGAGAATCACTTCCAGTGACCAAGCATCCAGGTGATGAGGTTTTCTCCGGTTCAACTTGCAAGCAGGGTGAAATTGAAGCTGTTGTTATTGCCACCGGTGTCCATACCTTCTTTGGGAAGGCTGCACATCTTGTGGACAGCACTAACCAAGTCGGACACTTCCAGAAGGTGCTCACTGCCATCGGGAACTTCTGTATCTGTTCCATTGCCATTGGAATGGTGATTGAAATCATAGTCATGTACCCAATTCAGCACCGCAAGTACAGGAGTGGAATTGACAATCTCTTGGTGCTCTTGATTGGAGGCATTCCCATTGCCATGCCCACTGTCTTGTCCGTGACAATGGCTATTGGTTCTCACAGGCTATCTCAGCAGGGTGCCATCACCAAGAGGATGACTGCCATTGAGGAAATGGCGGGTATGGATGTGCTTTGCAGTGATAAGACAGGAACACTAACTCTTAACATTTCGGGATTCCCGATTTGTCCCGAAATCCCGAAAATTTGGTTTGGGATTGGTATTGAATTTGGGATTCCCAAAAATTTCGGTTTGGGAATCGGGACAAGGGTTTCGGTATGGGATCCCATACCGAACCACCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

245

Amino Acids

26.04

Weight (kDa)

5.95

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1-E2_ATPase PF00122 12 - 89 3.4e-24 P-type ATPase actuator domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000545)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36571
pyrus_communis pycom02g01560 pycom08g10820 pycom09g05820 pycom13g21850 pycom14g16010
rosa_chinensis RchiOBHm_Chr2g0094861 RchiOBHm_Chr2g0124911 RchiOBHm_Chr2g0138581 RchiOBHm_Chr2g0160851 RchiOBHm_Chr4g0407411
rosa_laevigata RLG00000001652 RLG00000001872 RLG00000002037 RLG00000003076 RLG00000006419 RLG00000016771 RLG00000017383 RLG00000022851 RLG00000030563 RLG00000033869 RLG00000034176
rosa_multiflora Rmu_co8013424.1_g000001 Rmu_co8016716.1_g000001 Rmu_co8183472.1_g000001 Rmu_co8254047.1_g000001 Rmu_co8354891.1_g000001 Rmu_sc0000124.1_g000003 Rmu_sc0002132.1_g000027 Rmu_sc0003007.1_g000011 Rmu_sc0003221.1_g000096 Rmu_sc0003221.1_g000097 Rmu_sc0005119.1_g000004 Rmu_sc0008057.1_g000005 Rmu_sc0034532.1_g000001 Rmu_sc0036406.1_g000001 Rmu_ssc0000008.1_g000009
rosa_roxburghii Rroxscaffold_1G00030520 Rroxscaffold_2G00107360 Rroxscaffold_2G00153420 Rroxscaffold_5G00335750 Rroxscaffold_5G00351760 Rroxscaffold_5G00352080 Rroxscaffold_6G00419090
rosa_rugosa Rorug01G0341800 Rorug01G0341900 Rorug02G0180200 Rorug02G0180300 Rorug03G0186100 Rorug03G0186200 Rorug03G0284800 Rorug04G0104500 Rorug05G0246300 Rorug06G0108400 Rorug06G0471500 Rorug06G0510400
rosa_samantha Rh1AG159000 Rh1CG148200 Rh1CG189900 Rh2AG552800 Rh2BG473900 Rh2BG612100 Rh2BG612300 Rh2DG094500 Rh2DG482600 Rh3BG234000 Rh3CG320000 Rh4AG144200 Rh4BG127900 Rh4DG097300 Rh5AG128900 Rh5AG381200 Rh5BG363000 Rh5DG491900 Rh6AG048400 Rh6BG168100 Rh6CG005800 Rh6DG006000 Rh7AG255100 Rh7BG360600 Rh7CG271700 Rh7CG482300 Rh7DG451200
rosa_wichuraiana Rw4G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 509
AciI CCGC 3 cut(s) 17, 388, 548
AclWI GGATC 3 cut(s) 95, 707, 720
AcsI RAATTY 3 cut(s) 633, 656, 673
AfaI GTAC 2 cut(s) 374, 395
AfiI CCNNNNNNNGG 2 cut(s) 16, 246
AgeI ACCGGT 1 cut(s) 227
AgsI TTSAA 4 cut(s) 186, 209, 359, 656
AjnI CCWGG 1 cut(s) 160
AluBI AGCT 2 cut(s) 77, 212
AluI AGCT 2 cut(s) 77, 212
Alw21I GWGCWC 2 cut(s) 306, 426
AlwI GGATC 3 cut(s) 95, 707, 720
Ama87I CYCGRG 1 cut(s) 92
ApeKI GCWGC 1 cut(s) 254
ApoI RAATTY 3 cut(s) 633, 656, 673
ArsI GACNNNNNNTTYG 2 cut(s) 685, 717
AsiGI ACCGGT 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 141
AsuHPI GGTGA 5 cut(s) 110, 176, 212, 364, 508
AvaI CYCGRG 1 cut(s) 92
BaeI ACNNNNGTAYC 2 cut(s) 310, 343
BamHI GGATCC 1 cut(s) 712
BanI GGYRCC 1 cut(s) 509
BbsI GAAGAC 1 cut(s) 80
Bbv12I GWGCWC 2 cut(s) 306, 426
BbvI GCAGC 1 cut(s) 241
BccI CCATC 2 cut(s) 320, 521
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 1 cut(s) 162
BisI GCNGC 2 cut(s) 18, 255
BlsI GCNGC 2 cut(s) 19, 256
Bme1390I CCNGG 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 92
BmiI GGNNCC 2 cut(s) 511, 714
BmrFI CCNGG 1 cut(s) 162
BmsI GCATC 2 cut(s) 70, 166
BpiI GAAGAC 1 cut(s) 80
BplI GAGNNNNNCTC 2 cut(s) 161, 193
Bsa29I ATCGAT 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 359
BsaWI WCCGGW 2 cut(s) 179, 227
BsaXI ACNNNNNCTCC 2 cut(s) 54, 84
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 246
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 146
Bse3DI GCAATG 2 cut(s) 336, 444
Bse8I GATNNNNATC 1 cut(s) 359
BseBI CCWGG 1 cut(s) 162
BseCI ATCGAT 1 cut(s) 114
BseGI GGATG 3 cut(s) 157, 531, 562
BseJI GATNNNNATC 1 cut(s) 359
BseLI CCNNNNNNNGG 2 cut(s) 16, 246
BseMI GCAATG 2 cut(s) 336, 444
BseMII CTCAG 2 cut(s) 50, 515
BseNI ACTGG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 241
BseYI CCCAGC 1 cut(s) 73
BsgI GTGCAG 1 cut(s) 240
BshNI GGYRCC 1 cut(s) 509
BshTI ACCGGT 1 cut(s) 227
BshVI ATCGAT 1 cut(s) 114
BsiHKAI GWGCWC 2 cut(s) 306, 426
BsiHKCI CYCGRG 1 cut(s) 92
BsiSI CCGG 2 cut(s) 180, 228
BslFI GGGAC 3 cut(s) 56, 603, 706
BslI CCNNNNNNNGG 2 cut(s) 16, 246
BsmFI GGGAC 3 cut(s) 56, 603, 706
BsmI GAATGC 2 cut(s) 24, 438
BsoBI CYCGRG 1 cut(s) 92
Bsp1286I GDGCHC 2 cut(s) 306, 426
Bsp143I GATC 5 cut(s) 3, 100, 111, 115, 712
BspACI CCGC 3 cut(s) 17, 388, 548
BspCNI CTCAG 2 cut(s) 49, 514
BspDI ATCGAT 1 cut(s) 114
BspLI GGNNCC 2 cut(s) 511, 714
BspPI GGATC 3 cut(s) 95, 707, 720
BspT107I GGYRCC 1 cut(s) 509
BsrDI GCAATG 2 cut(s) 336, 444
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 146
BssAI RCCGGY 1 cut(s) 227
BssMI GATC 5 cut(s) 3, 100, 111, 115, 712
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 22, 194
BstDEI CTNAG 2 cut(s) 36, 501
BstF5I GGATG 3 cut(s) 157, 531, 562
BstKTI GATC 5 cut(s) 6, 103, 114, 118, 715
BstMBI GATC 5 cut(s) 3, 100, 111, 115, 712
BstMWI GCNNNNNNNGC 1 cut(s) 502
BstNI CCWGG 1 cut(s) 162
BstNSI RCATGY 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 160
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 1 cut(s) 712
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 1 cut(s) 712
Bsu15I ATCGAT 1 cut(s) 114
BsuTUI ATCGAT 1 cut(s) 114
BtsCI GGATG 3 cut(s) 157, 531, 562
BtsI GCAGTG 2 cut(s) 306, 574
BtsIMutI CAGTG 4 cut(s) 153, 306, 456, 574
Cac8I GCNNGC 2 cut(s) 22, 194
Cfr10I RCCGGY 1 cut(s) 227
ClaI ATCGAT 1 cut(s) 114
Csp6I GTAC 2 cut(s) 373, 394
CspAI ACCGGT 1 cut(s) 227
CviAII CATG 3 cut(s) 21, 370, 451
CviJI RGCY 5 cut(s) 77, 212, 254, 479, 496
CviKI_1 RGCY 5 cut(s) 77, 212, 254, 479, 496
CviQI GTAC 2 cut(s) 373, 394
DdeI CTNAG 2 cut(s) 36, 501
DpnI GATC 5 cut(s) 5, 102, 113, 117, 714
DpnII GATC 5 cut(s) 3, 100, 111, 115, 712
DraI TTTAAA 1 cut(s) 108
Eco32I GATATC 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 160
EcoRV GATATC 1 cut(s) 67
EcoT22I ATGCAT 1 cut(s) 26
FaeI CATG 3 cut(s) 24, 373, 454
FaiI YATR 8 cut(s) 22, 237, 365, 371, 452, 554, 709, 719
FaqI GGGAC 3 cut(s) 56, 603, 706
FatI CATG 3 cut(s) 20, 369, 450
FauI CCCGC 1 cut(s) 541
Fnu4HI GCNGC 2 cut(s) 18, 255
FokI GGATG 3 cut(s) 144, 538, 569
Fsp4HI GCNGC 2 cut(s) 18, 255
GluI GCNGC 2 cut(s) 18, 255
GsaI CCCAGC 1 cut(s) 77
HapII CCGG 2 cut(s) 180, 228
Hin1II CATG 3 cut(s) 24, 373, 454
HinfI GANTC 4 cut(s) 137, 606, 664, 687
HpaII CCGG 2 cut(s) 180, 228
HphI GGTGA 5 cut(s) 110, 176, 212, 364, 508
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 1 cut(s) 287
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 3 cut(s) 244, 250, 292
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4V TGCA 4 cut(s) 24, 192, 257, 567
HpyF10VI GCNNNNNNNGC 1 cut(s) 502
HpyF3I CTNAG 2 cut(s) 36, 501
Hsp92II CATG 3 cut(s) 24, 373, 454
Kzo9I GATC 5 cut(s) 3, 100, 111, 115, 712
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 2 cut(s) 70, 166
MaeIII GTNAC 2 cut(s) 148, 469
MalI GATC 5 cut(s) 5, 102, 113, 117, 714
MboI GATC 5 cut(s) 3, 100, 111, 115, 712
MboII GAAGA 1 cut(s) 80
MflI RGATCY 1 cut(s) 712
MhlI GDGCHC 2 cut(s) 306, 426
MluCI AATT 7 cut(s) 56, 204, 378, 405, 633, 656, 673
MmeI TCCRAC 1 cut(s) 265
MnlI CCTC 7 cut(s) 17, 88, 137, 163, 428, 516, 532
Mph1103I ATGCAT 1 cut(s) 26
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 2 cut(s) 107, 594
MslI CAYNNNNRTG 4 cut(s) 162, 347, 368, 449
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 2 cut(s) 180, 228
MspR9I CCNGG 1 cut(s) 162
Mva1269I GAATGC 2 cut(s) 24, 438
MvaI CCWGG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 502
NdeII GATC 5 cut(s) 3, 100, 111, 115, 712
NlaIII CATG 3 cut(s) 24, 373, 454
NlaIV GGNNCC 2 cut(s) 511, 714
NmuCI GTSAC 2 cut(s) 148, 469
NsiI ATGCAT 1 cut(s) 26
NspI RCATGY 1 cut(s) 24
PaeI GCATGC 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 608
PctI GAATGC 2 cut(s) 24, 438
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 4 cut(s) 137, 606, 664, 687
PinAI ACCGGT 1 cut(s) 227
PkrI GCNGC 2 cut(s) 19, 256
Psp6I CCWGG 1 cut(s) 160
PspFI CCCAGC 1 cut(s) 73
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 2 cut(s) 511, 714
PsuI RGATCY 1 cut(s) 712
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 2 cut(s) 374, 395
RsaNI GTAC 2 cut(s) 373, 394
RseI CAYNNNNRTG 4 cut(s) 162, 347, 368, 449
SaqAI TTAA 2 cut(s) 107, 594
SatI GCNGC 2 cut(s) 18, 255
Sau3AI GATC 5 cut(s) 3, 100, 111, 115, 712
ScrFI CCNGG 1 cut(s) 162
SduI GDGCHC 2 cut(s) 306, 426
SetI ASST 6 cut(s) 79, 166, 174, 214, 242, 303
SfaNI GCATC 2 cut(s) 70, 166
Sfr274I CTCGAG 1 cut(s) 92
SgrAI CRCCGGYG 1 cut(s) 227
SlaI CTCGAG 1 cut(s) 92
SmiMI CAYNNNNRTG 4 cut(s) 162, 347, 368, 449
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SphI GCATGC 1 cut(s) 24
Sse9I AATT 7 cut(s) 56, 204, 378, 405, 633, 656, 673
SsiI CCGC 3 cut(s) 17, 388, 548
StyD4I CCNGG 1 cut(s) 160
TaaI ACNGT 1 cut(s) 460
TaqI TCGA 2 cut(s) 93, 114
TasI AATT 7 cut(s) 56, 204, 378, 405, 633, 656, 673
TatI WGTACW 1 cut(s) 393
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 4 cut(s) 137, 606, 664, 687
Tru1I TTAA 2 cut(s) 107, 594
Tru9I TTAA 2 cut(s) 107, 594
TscAI CASTG 4 cut(s) 153, 313, 463, 574
TseFI GTSAC 2 cut(s) 148, 469
TseI GCWGC 1 cut(s) 254
Tsp45I GTSAC 2 cut(s) 148, 469
TspGWI ACGGA 1 cut(s) 457
TspRI CASTG 4 cut(s) 153, 313, 463, 574
XapI RAATTY 3 cut(s) 633, 656, 673
XceI RCATGY 1 cut(s) 24
XcmI CCANNNNNNNNNTGG 2 cut(s) 242, 541
XhoI CTCGAG 1 cut(s) 92
XmnI GAANNNNTTC 1 cut(s) 141
Zsp2I ATGCAT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.