Rroxscaffold_6G00419090

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000545
Physical Location & Seq
Forward (+)
40614799 .. 40619843
5045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00419090.1

Sequence Viewer

Length: 462 bp
ATGGAAGAAGTTTTTGACCATCTTAAATGCACAAAAGATGGTTTGAGCTCCGATGAAGTTAAAGAGCGGTTGAATTTATTTGGTTATAATAAGCTTGAAGAAAAGAAGGTTTTACGTGACAGAAAATGGACAGAAAAAGATGCGGCTAGATTGGTTCCTGGAGACATAATTAGCATCAAACTTGGTGATATCGTTCTATCCGGTGCACGCCTTCTTGAAGGTGATCCTTTAAAGATTGATCAGTCTGCTCTTAACGGAGAATCGCTTCCTGTGACCAAAAATCCTGGTGATGTGGTTTACCGAGGATCAACATGCAAACAAGGAGAACTTGAAGGAGTTGTTATTACAACTGGTATCCATACTTTCTTTGGAAACTCAGCTGATCTTGTGGAGAAAACAATACATGTTAGCCGTTTCCAGAAGGGTACCTGCACTTCAAATTATACAAATCCCTTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

153

Amino Acids

16.88

Weight (kDa)

5.94

Isoelectric Point (pI)

20.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 1 - 36 1.8e-07 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 36 - 131 1.2e-24 P-type ATPase actuator domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000545)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36571
pyrus_communis pycom02g01560 pycom08g10820 pycom09g05820 pycom13g21850 pycom14g16010
rosa_chinensis RchiOBHm_Chr2g0094861 RchiOBHm_Chr2g0124911 RchiOBHm_Chr2g0138581 RchiOBHm_Chr2g0160851 RchiOBHm_Chr4g0407411
rosa_laevigata RLG00000001652 RLG00000001872 RLG00000002037 RLG00000003076 RLG00000006419 RLG00000016771 RLG00000017383 RLG00000022851 RLG00000030563 RLG00000033869 RLG00000034176
rosa_multiflora Rmu_co8013424.1_g000001 Rmu_co8016716.1_g000001 Rmu_co8183472.1_g000001 Rmu_co8254047.1_g000001 Rmu_co8354891.1_g000001 Rmu_sc0000124.1_g000003 Rmu_sc0002132.1_g000027 Rmu_sc0003007.1_g000011 Rmu_sc0003221.1_g000096 Rmu_sc0003221.1_g000097 Rmu_sc0005119.1_g000004 Rmu_sc0008057.1_g000005 Rmu_sc0034532.1_g000001 Rmu_sc0036406.1_g000001 Rmu_ssc0000008.1_g000009
rosa_roxburghii Rroxscaffold_1G00030520 Rroxscaffold_2G00107360 Rroxscaffold_2G00153420 Rroxscaffold_5G00335750 Rroxscaffold_5G00351760 Rroxscaffold_5G00352080 Rroxscaffold_6G00419090
rosa_rugosa Rorug01G0341800 Rorug01G0341900 Rorug02G0180200 Rorug02G0180300 Rorug03G0186100 Rorug03G0186200 Rorug03G0284800 Rorug04G0104500 Rorug05G0246300 Rorug06G0108400 Rorug06G0471500 Rorug06G0510400
rosa_samantha Rh1AG159000 Rh1CG148200 Rh1CG189900 Rh2AG552800 Rh2BG473900 Rh2BG612100 Rh2BG612300 Rh2DG094500 Rh2DG482600 Rh3BG234000 Rh3CG320000 Rh4AG144200 Rh4BG127900 Rh4DG097300 Rh5AG128900 Rh5AG381200 Rh5BG363000 Rh5DG491900 Rh6AG048400 Rh6BG168100 Rh6CG005800 Rh6DG006000 Rh7AG255100 Rh7BG360600 Rh7CG271700 Rh7CG482300 Rh7DG451200
rosa_wichuraiana Rw4G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 437
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 425
AccBSI CCGCTC 1 cut(s) 67
AciI CCGC 2 cut(s) 67, 143
AclWI GGATC 2 cut(s) 218, 313
AcsI RAATTY 1 cut(s) 73
AfaI GTAC 1 cut(s) 427
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 5 cut(s) 73, 98, 218, 332, 438
AjnI CCWGG 2 cut(s) 157, 283
AluBI AGCT 3 cut(s) 48, 94, 380
AluI AGCT 3 cut(s) 48, 94, 380
Alw21I GWGCWC 2 cut(s) 50, 208
Alw26I GTCTC 1 cut(s) 156
Alw44I GTGCAC 1 cut(s) 204
AlwI GGATC 2 cut(s) 218, 313
ApaLI GTGCAC 1 cut(s) 204
ApoI RAATTY 1 cut(s) 73
Asp700I GAANNNNTTC 1 cut(s) 264
Asp718I GGTACC 1 cut(s) 425
AsuHPI GGTGA 3 cut(s) 197, 233, 299
BaeGI GKGCMC 1 cut(s) 208
BanI GGYRCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 50
BarI GAAGNNNNNNTAC 2 cut(s) 418, 450
Bbv12I GWGCWC 2 cut(s) 50, 208
BccI CCATC 2 cut(s) 27, 32
BceAI ACGGC 1 cut(s) 396
BciT130I CCWGG 2 cut(s) 159, 285
BciVI GTATCC 1 cut(s) 365
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 1 cut(s) 147
BfuAI ACCTGC 1 cut(s) 437
BfuI GTATCC 1 cut(s) 365
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
Bme1390I CCNGG 2 cut(s) 159, 285
BmiI GGNNCC 2 cut(s) 156, 427
BmrFI CCNGG 2 cut(s) 159, 285
BmsI GCATC 2 cut(s) 130, 183
BpmI CTGGAG 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 301, 453
BsaWI WCCGGW 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 355
BseBI CCWGG 2 cut(s) 159, 285
BseDI CCNNGG 2 cut(s) 301, 453
BseMII CTCAG 1 cut(s) 390
BseNI ACTGG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 208
BsgI GTGCAG 1 cut(s) 415
BshNI GGYRCC 1 cut(s) 425
BsiHKAI GWGCWC 2 cut(s) 50, 208
BsiSI CCGG 1 cut(s) 201
BsmAI GTCTC 1 cut(s) 156
Bsp1286I GDGCHC 2 cut(s) 50, 208
Bsp143I GATC 4 cut(s) 223, 238, 305, 382
BspACI CCGC 2 cut(s) 67, 143
BspCNI CTCAG 1 cut(s) 389
BspLI GGNNCC 2 cut(s) 156, 427
BspMI ACCTGC 1 cut(s) 437
BspPI GGATC 2 cut(s) 218, 313
BspT107I GGYRCC 1 cut(s) 425
BsrBI CCGCTC 1 cut(s) 67
BsrI ACTGG 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 301, 453
BssMI GATC 4 cut(s) 223, 238, 305, 382
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 159, 285
BstBAI YACGTR 1 cut(s) 116
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 376
BstKTI GATC 4 cut(s) 226, 241, 308, 385
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 4 cut(s) 223, 238, 305, 382
BstNI CCWGG 2 cut(s) 159, 285
BstNSI RCATGY 2 cut(s) 315, 407
BstSCI CCNGG 2 cut(s) 157, 283
BstSLI GKGCMC 1 cut(s) 208
BsuI GTATCC 1 cut(s) 365
BveI ACCTGC 1 cut(s) 437
Cac8I GCNNGC 1 cut(s) 208
Csp6I GTAC 1 cut(s) 426
CviAII CATG 3 cut(s) 312, 404, 459
CviJI RGCY 5 cut(s) 48, 94, 146, 380, 411
CviKI_1 RGCY 5 cut(s) 48, 94, 146, 380, 411
CviQI GTAC 1 cut(s) 426
DdeI CTNAG 1 cut(s) 376
DpnI GATC 4 cut(s) 225, 240, 307, 384
DpnII GATC 4 cut(s) 223, 238, 305, 382
DraI TTTAAA 1 cut(s) 231
Ecl136II GAGCTC 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 50
Eco32I GATATC 1 cut(s) 190
Eco53kI GAGCTC 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 48
EcoRII CCWGG 2 cut(s) 157, 283
EcoRV GATATC 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 3 cut(s) 315, 407, 462
FaiI YATR 7 cut(s) 87, 167, 313, 360, 405, 444, 460
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FatI CATG 3 cut(s) 311, 403, 458
FbaI TGATCA 1 cut(s) 238
Fnu4HI GCNGC 1 cut(s) 144
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 1 cut(s) 147
GluI GCNGC 1 cut(s) 144
GsuI CTGGAG 1 cut(s) 180
HapII CCGG 1 cut(s) 201
Hin1II CATG 3 cut(s) 315, 407, 462
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 1 cut(s) 260
HpaII CCGG 1 cut(s) 201
HphI GGTGA 3 cut(s) 197, 233, 299
Hpy166II GTNNAC 2 cut(s) 206, 298
Hpy188I TCNGA 1 cut(s) 52
Hpy188III TCNNGA 2 cut(s) 215, 418
Hpy8I GTNNAC 2 cut(s) 206, 298
HpyAV CCTTC 5 cut(s) 100, 212, 221, 326, 415
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 4 cut(s) 30, 206, 315, 432
HpyF3I CTNAG 1 cut(s) 376
HpySE526I ACGT 1 cut(s) 115
Hsp92II CATG 3 cut(s) 315, 407, 462
KpnI GGTACC 1 cut(s) 429
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 4 cut(s) 223, 238, 305, 382
LmnI GCTCC 1 cut(s) 53
LpnPI CCDG 9 cut(s) 144, 171, 214, 270, 282, 297, 336, 431, 442
LweI GCATC 2 cut(s) 130, 183
MaeI CTAG 1 cut(s) 147
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 2 cut(s) 116, 271
MalI GATC 4 cut(s) 225, 240, 307, 384
MbiI CCGCTC 1 cut(s) 67
MboI GATC 4 cut(s) 223, 238, 305, 382
MboII GAAGA 2 cut(s) 17, 110
MhlI GDGCHC 2 cut(s) 50, 208
MluCI AATT 3 cut(s) 73, 168, 439
MnlI CCTC 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 264
MseI TTAA 4 cut(s) 24, 60, 230, 252
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 2 cut(s) 159, 285
MvaI CCWGG 2 cut(s) 159, 285
NdeII GATC 4 cut(s) 223, 238, 305, 382
NlaIII CATG 3 cut(s) 315, 407, 462
NlaIV GGNNCC 2 cut(s) 156, 427
NmuCI GTSAC 2 cut(s) 116, 271
NspI RCATGY 2 cut(s) 315, 407
PciI ACATGT 1 cut(s) 403
PdmI GAANNNNTTC 1 cut(s) 264
PfeI GAWTC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 157
PkrI GCNGC 1 cut(s) 145
Ppu21I YACGTR 1 cut(s) 116
PscI ACATGT 1 cut(s) 403
PsiI TTATAA 1 cut(s) 87
Psp124BI GAGCTC 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 157, 283
PspGI CCWGG 2 cut(s) 157, 283
PspN4I GGNNCC 2 cut(s) 156, 427
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 1 cut(s) 427
RsaNI GTAC 1 cut(s) 426
SacI GAGCTC 1 cut(s) 50
SaqAI TTAA 4 cut(s) 24, 60, 230, 252
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 4 cut(s) 223, 238, 305, 382
ScrFI CCNGG 2 cut(s) 159, 285
SduI GDGCHC 2 cut(s) 50, 208
SetI ASST 7 cut(s) 50, 96, 111, 118, 223, 382, 431
SfaNI GCATC 2 cut(s) 130, 183
Sse9I AATT 3 cut(s) 73, 168, 439
SsiI CCGC 2 cut(s) 67, 143
SspMI CTAG 1 cut(s) 147
SstI GAGCTC 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 157, 283
StyI CCWWGG 1 cut(s) 453
TaiI ACGT 1 cut(s) 118
TasI AATT 3 cut(s) 73, 168, 439
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 4 cut(s) 24, 60, 230, 252
Tru9I TTAA 4 cut(s) 24, 60, 230, 252
TseFI GTSAC 2 cut(s) 116, 271
Tsp45I GTSAC 2 cut(s) 116, 271
TspDTI ATGAA 1 cut(s) 69
TspGWI ACGGA 1 cut(s) 270
VneI GTGCAC 1 cut(s) 204
XapI RAATTY 1 cut(s) 73
XceI RCATGY 2 cut(s) 315, 407
XcmI CCANNNNNNNNNTGG 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 264
XspI CTAG 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.