Rh6BG168100

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000545
Physical Location & Seq
Reverse (-)
27581277 .. 27583044
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG168100.1

Sequence Viewer

Length: 747 bp
ATGGAAGAAGTTTTTGACCATCTTAAATGCACAAAAGATGGTTTGAGCTCTGATGAAATTAAAGAGCGGTTGAATTTATTTGGTTATAATAAGCTTGAAGAAAAGAAGGAAAGCAAACTTTTGAAGTTTTTGGGCTTTATGTGGAATCCTCTGTCCTGGTTCATGGAAGCTGCAGCTATCCTAGCCATTGTTTTTAGCCAAGGAGGGGCAAATCTACACTATAACCCTTGCTCCTCTTTTTTTTCCTTATCTTTCCCCTCTCTTCCTGTAGAGTACAAAGTGTTGAAAACAAGAAGTTTTATAGAGGAAAACAATGCAGGCAATGCTGCTGCTGCTCTTATGGCTCAGTCGGCCCCAAAAGCGAAGGTTTTACGTGACAGAAAATGGACAGAAAAAGATGCGGCTAGATTGGTTCCTGGAGACATAATTAGCATCAAACTTGGTGATATCGTTCTATCTGGTGCACGCCTTCTTGAAGGTGATCCTTTAAAGATTGATCAGCTTTTAACATCAATTGGATACTTCTGCATCTATTCAATTGTTGTTGGAATGTTTATTGGAATACCAGTTGGAATTCAGAGGAGGGAGAGTGGTGGTGCTGCTGCTGTTATTGCAAACCTACTCGTCATCCTAATTGGTGGGATCCCTATTGCTATGCCAACTGTTCTTTCCATTATGATGACCAGTGGTTCACATCGCTTGTCTCAGCCGGAACATGAGCTAATCTTGAGTCTCGAGTCAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

248

Amino Acids

27.13

Weight (kDa)

6.44

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 1 - 61 2.1e-14 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 117 - 167 3.7e-08 P-type ATPase actuator domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000545)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36571
pyrus_communis pycom02g01560 pycom08g10820 pycom09g05820 pycom13g21850 pycom14g16010
rosa_chinensis RchiOBHm_Chr2g0094861 RchiOBHm_Chr2g0124911 RchiOBHm_Chr2g0138581 RchiOBHm_Chr2g0160851 RchiOBHm_Chr4g0407411
rosa_laevigata RLG00000001652 RLG00000001872 RLG00000002037 RLG00000003076 RLG00000006419 RLG00000016771 RLG00000017383 RLG00000022851 RLG00000030563 RLG00000033869 RLG00000034176
rosa_multiflora Rmu_co8013424.1_g000001 Rmu_co8016716.1_g000001 Rmu_co8183472.1_g000001 Rmu_co8254047.1_g000001 Rmu_co8354891.1_g000001 Rmu_sc0000124.1_g000003 Rmu_sc0002132.1_g000027 Rmu_sc0003007.1_g000011 Rmu_sc0003221.1_g000096 Rmu_sc0003221.1_g000097 Rmu_sc0005119.1_g000004 Rmu_sc0008057.1_g000005 Rmu_sc0034532.1_g000001 Rmu_sc0036406.1_g000001 Rmu_ssc0000008.1_g000009
rosa_roxburghii Rroxscaffold_1G00030520 Rroxscaffold_2G00107360 Rroxscaffold_2G00153420 Rroxscaffold_5G00335750 Rroxscaffold_5G00351760 Rroxscaffold_5G00352080 Rroxscaffold_6G00419090
rosa_rugosa Rorug01G0341800 Rorug01G0341900 Rorug02G0180200 Rorug02G0180300 Rorug03G0186100 Rorug03G0186200 Rorug03G0284800 Rorug04G0104500 Rorug05G0246300 Rorug06G0108400 Rorug06G0471500 Rorug06G0510400
rosa_samantha Rh1AG159000 Rh1CG148200 Rh1CG189900 Rh2AG552800 Rh2BG473900 Rh2BG612100 Rh2BG612300 Rh2DG094500 Rh2DG482600 Rh3BG234000 Rh3CG320000 Rh4AG144200 Rh4BG127900 Rh4DG097300 Rh5AG128900 Rh5AG381200 Rh5BG363000 Rh5DG491900 Rh6AG048400 Rh6BG168100 Rh6CG005800 Rh6DG006000 Rh7AG255100 Rh7BG360600 Rh7CG271700 Rh7CG482300 Rh7DG451200
rosa_wichuraiana Rw4G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 87
AccBSI CCGCTC 1 cut(s) 67
AciI CCGC 2 cut(s) 67, 401
AclWI GGATC 3 cut(s) 476, 637, 650
AcsI RAATTY 2 cut(s) 73, 573
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 205
AgsI TTSAA 6 cut(s) 73, 98, 124, 286, 476, 537
AjnI CCWGG 2 cut(s) 155, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 652, 684
AluBI AGCT 6 cut(s) 48, 94, 170, 176, 502, 721
AluI AGCT 6 cut(s) 48, 94, 170, 176, 502, 721
Alw21I GWGCWC 2 cut(s) 50, 466
Alw26I GTCTC 3 cut(s) 414, 708, 737
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 3 cut(s) 476, 637, 650
Ama87I CYCGRG 1 cut(s) 734
AoxI GGCC 1 cut(s) 351
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 7 cut(s) 170, 173, 326, 329, 332, 599, 602
ApoI RAATTY 2 cut(s) 73, 573
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 2 cut(s) 455, 491
AvaI CYCGRG 1 cut(s) 734
BaeGI GKGCMC 1 cut(s) 466
BamHI GGATCC 1 cut(s) 642
BanII GRGCYC 1 cut(s) 50
Bbv12I GWGCWC 2 cut(s) 50, 466
BbvI GCAGC 7 cut(s) 157, 185, 313, 316, 319, 586, 589
BccI CCATC 2 cut(s) 27, 32
BciT130I CCWGG 2 cut(s) 157, 417
BciVI GTATCC 1 cut(s) 512
BclI TGATCA 1 cut(s) 496
BcoDI GTCTC 3 cut(s) 414, 708, 737
BfaI CTAG 2 cut(s) 182, 405
BfmI CTRYAG 2 cut(s) 171, 267
BfuI GTATCC 1 cut(s) 512
BisI GCNGC 8 cut(s) 171, 174, 327, 330, 333, 402, 600, 603
BlsI GCNGC 8 cut(s) 172, 175, 328, 331, 334, 403, 601, 604
Bme1390I CCNGG 2 cut(s) 157, 417
BmeT110I CYCGRG 1 cut(s) 734
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 3 cut(s) 354, 414, 644
BmrFI CCNGG 2 cut(s) 157, 417
BmsI GCATC 3 cut(s) 388, 441, 537
BpmI CTGGAG 1 cut(s) 438
BsaAI YACGTR 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 199
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 574, 604
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse1I ACTGG 2 cut(s) 566, 684
Bse3DI GCAATG 1 cut(s) 328
BseBI CCWGG 2 cut(s) 157, 417
BseDI CCNNGG 1 cut(s) 199
BseGI GGATG 1 cut(s) 627
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 2 cut(s) 359, 719
BseNI ACTGG 2 cut(s) 566, 684
BseRI GAGGAG 2 cut(s) 223, 595
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 7 cut(s) 157, 185, 313, 316, 319, 586, 589
BshFI GGCC 1 cut(s) 353
BsiHKAI GWGCWC 2 cut(s) 50, 466
BsiHKCI CYCGRG 1 cut(s) 734
BsiSI CCGG 1 cut(s) 710
BslI CCNNNNNNNGG 1 cut(s) 205
BsmAI GTCTC 3 cut(s) 414, 708, 737
BsnI GGCC 1 cut(s) 353
BsoBI CYCGRG 1 cut(s) 734
Bsp1286I GDGCHC 2 cut(s) 50, 466
Bsp143I GATC 3 cut(s) 481, 496, 642
BspACI CCGC 2 cut(s) 67, 401
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 2 cut(s) 358, 718
BspLI GGNNCC 3 cut(s) 354, 414, 644
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 3 cut(s) 476, 637, 650
BsrBI CCGCTC 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 328
BsrI ACTGG 2 cut(s) 566, 684
BssECI CCNNGG 1 cut(s) 199
BssMI GATC 3 cut(s) 481, 496, 642
BssT1I CCWWGG 1 cut(s) 199
Bst2UI CCWGG 2 cut(s) 157, 417
Bst4CI ACNGT 1 cut(s) 664
Bst6I CTCTTC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 319, 466
BstDEI CTNAG 2 cut(s) 345, 705
BstF5I GGATG 1 cut(s) 627
BstKTI GATC 3 cut(s) 484, 499, 645
BstMAI GTCTC 3 cut(s) 414, 708, 737
BstMBI GATC 3 cut(s) 481, 496, 642
BstMWI GCNNNNNNNGC 7 cut(s) 182, 323, 332, 341, 350, 359, 611
BstNI CCWGG 2 cut(s) 157, 417
BstSCI CCNGG 2 cut(s) 155, 415
BstSFI CTRYAG 2 cut(s) 171, 267
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 7 cut(s) 157, 185, 313, 316, 319, 586, 589
BstX2I RGATCY 1 cut(s) 642
BstYI RGATCY 1 cut(s) 642
BsuI GTATCC 1 cut(s) 512
BsuRI GGCC 1 cut(s) 353
BtgZI GCGATG 1 cut(s) 680
BtsCI GGATG 1 cut(s) 627
BtsIMutI CAGTG 1 cut(s) 691
Cac8I GCNNGC 2 cut(s) 319, 466
Cfr13I GGNCC 1 cut(s) 352
Csp6I GTAC 1 cut(s) 274
CviAII CATG 2 cut(s) 163, 716
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 2 cut(s) 345, 705
DpnI GATC 3 cut(s) 483, 498, 644
DpnII GATC 3 cut(s) 481, 496, 642
DraI TTTAAA 1 cut(s) 489
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 199
Eco24I GRGCYC 1 cut(s) 50
Eco32I GATATC 1 cut(s) 448
Eco53kI GAGCTC 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 734
EcoICRI GAGCTC 1 cut(s) 48
EcoRI GAATTC 1 cut(s) 573
EcoRII CCWGG 2 cut(s) 155, 415
EcoRV GATATC 1 cut(s) 448
EcoT14I CCWWGG 1 cut(s) 199
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 199
FaeI CATG 2 cut(s) 166, 719
FatI CATG 2 cut(s) 162, 715
FbaI TGATCA 1 cut(s) 496
Fnu4HI GCNGC 8 cut(s) 171, 174, 327, 330, 333, 402, 600, 603
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 8 cut(s) 171, 174, 327, 330, 333, 402, 600, 603
FspBI CTAG 2 cut(s) 182, 405
GluI GCNGC 8 cut(s) 171, 174, 327, 330, 333, 402, 600, 603
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 1 cut(s) 353
HapII CCGG 1 cut(s) 710
Hin1II CATG 2 cut(s) 166, 719
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 3 cut(s) 145, 730, 737
HpaII CCGG 1 cut(s) 710
HphI GGTGA 2 cut(s) 455, 491
Hpy166II GTNNAC 2 cut(s) 464, 692
Hpy188I TCNGA 2 cut(s) 52, 579
Hpy188III TCNNGA 4 cut(s) 473, 727, 734, 741
Hpy8I GTNNAC 2 cut(s) 464, 692
HpyAV CCTTC 4 cut(s) 100, 358, 470, 479
HpyCH4III ACNGT 1 cut(s) 664
HpyCH4IV ACGT 1 cut(s) 373
HpyCH4V TGCA 6 cut(s) 30, 173, 317, 464, 528, 614
HpyF10VI GCNNNNNNNGC 7 cut(s) 182, 323, 332, 341, 350, 359, 611
HpyF3I CTNAG 2 cut(s) 345, 705
HpySE526I ACGT 1 cut(s) 373
Hsp92II CATG 2 cut(s) 166, 719
Ksp22I TGATCA 1 cut(s) 496
Kzo9I GATC 3 cut(s) 481, 496, 642
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 7 cut(s) 157, 185, 313, 316, 319, 586, 589
LweI GCATC 3 cut(s) 388, 441, 537
MaeI CTAG 2 cut(s) 182, 405
MaeII ACGT 1 cut(s) 373
MaeIII GTNAC 1 cut(s) 374
MalI GATC 3 cut(s) 483, 498, 644
MbiI CCGCTC 1 cut(s) 67
MboI GATC 3 cut(s) 481, 496, 642
MboII GAAGA 3 cut(s) 17, 110, 254
MfeI CAATTG 2 cut(s) 513, 537
MflI RGATCY 1 cut(s) 642
MhlI GDGCHC 2 cut(s) 50, 466
MluCI AATT 7 cut(s) 57, 73, 426, 513, 537, 573, 633
MlyI GAGTC 2 cut(s) 739, 746
MmeI TCCRAC 2 cut(s) 526, 550
MnlI CCTC 7 cut(s) 159, 197, 244, 268, 298, 573, 576
MseI TTAA 4 cut(s) 24, 60, 488, 506
MslI CAYNNNNRTG 1 cut(s) 677
MspI CCGG 1 cut(s) 710
MspR9I CCNGG 2 cut(s) 157, 417
MunI CAATTG 2 cut(s) 513, 537
MvaI CCWGG 2 cut(s) 157, 417
MwoI GCNNNNNNNGC 7 cut(s) 182, 323, 332, 341, 350, 359, 611
NdeII GATC 3 cut(s) 481, 496, 642
NlaIII CATG 2 cut(s) 166, 719
NlaIV GGNNCC 3 cut(s) 354, 414, 644
NmuCI GTSAC 1 cut(s) 374
PaeR7I CTCGAG 1 cut(s) 734
PfeI GAWTC 1 cut(s) 145
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 8 cut(s) 172, 175, 328, 331, 334, 403, 601, 604
PleI GAGTC 2 cut(s) 738, 745
PpsI GAGTC 2 cut(s) 738, 745
Ppu21I YACGTR 1 cut(s) 374
PsiI TTATAA 1 cut(s) 87
Psp124BI GAGCTC 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 155, 415
PspGI CCWGG 2 cut(s) 155, 415
PspN4I GGNNCC 3 cut(s) 354, 414, 644
PspPI GGNCC 1 cut(s) 352
PstI CTGCAG 1 cut(s) 175
PsuI RGATCY 1 cut(s) 642
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 677
SacI GAGCTC 1 cut(s) 50
SaqAI TTAA 4 cut(s) 24, 60, 488, 506
SatI GCNGC 8 cut(s) 171, 174, 327, 330, 333, 402, 600, 603
Sau3AI GATC 3 cut(s) 481, 496, 642
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 2 cut(s) 739, 746
ScrFI CCNGG 2 cut(s) 157, 417
SduI GDGCHC 2 cut(s) 50, 466
SfaNI GCATC 3 cut(s) 388, 441, 537
SfcI CTRYAG 2 cut(s) 171, 267
Sfr274I CTCGAG 1 cut(s) 734
SlaI CTCGAG 1 cut(s) 734
SmiMI CAYNNNNRTG 1 cut(s) 677
SmlI CTYRAG 2 cut(s) 727, 734
SmoI CTYRAG 2 cut(s) 727, 734
Sse9I AATT 7 cut(s) 57, 73, 426, 513, 537, 573, 633
SsiI CCGC 2 cut(s) 67, 401
SspMI CTAG 2 cut(s) 182, 405
SstI GAGCTC 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 155, 415
StyI CCWWGG 1 cut(s) 199
TaaI ACNGT 1 cut(s) 664
TaiI ACGT 1 cut(s) 376
TaqI TCGA 1 cut(s) 735
TasI AATT 7 cut(s) 57, 73, 426, 513, 537, 573, 633
TatI WGTACW 1 cut(s) 273
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 1 cut(s) 145
Tru1I TTAA 4 cut(s) 24, 60, 488, 506
Tru9I TTAA 4 cut(s) 24, 60, 488, 506
TscAI CASTG 1 cut(s) 691
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 7 cut(s) 170, 173, 326, 329, 332, 599, 602
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 2 cut(s) 69, 151
TspRI CASTG 1 cut(s) 691
VneI GTGCAC 1 cut(s) 462
XapI RAATTY 2 cut(s) 73, 573
XhoI CTCGAG 1 cut(s) 734
XspI CTAG 2 cut(s) 182, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.