Rroxscaffold_2G00153420

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000545
Physical Location & Seq
Reverse (-)
90207667 .. 90209753
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153420.1

Sequence Viewer

Length: 786 bp
ATGCACAAAAGATGGTTTGAGCTCGATGAAGTTAAAGAGCGGTTGAATTTATTTGGTTATAATAAGCTTGAAGAAAAGAAGGTCGTGATGCAAATGTTAAGCATGAATCCTAGATTACTACATGGTGACGTCTCCTTTTTTGTGCATATATATGATGCAAGTTATCATGATTTTGTTGGTATCATCATCCTACTTATTATTAATTCTACCATCAGTTTTATAGAGGAAAACAATGCAGGCAATGCTGCTGCTGCTCTTATGGCTCGGTCGGCCCCAAAAGCGAAGGTTTTACGTGACGGAAAATGGACAGAAAAAGATGCGGCTAGATTGGTTCCTGGAGACATAATTAGCATCAAACTTGGTGATATCGTTCTATCTCGTTTTATAGAGGAAAACAATGCAGGCAATGCTGCTGCTCTTATGGCTCGGTCGGCCCCAAAAGCGAAGGTTTTACGTGACGGAAAATGGACAGAAAAAGATGCGGCTAGATTGGTTCCTGGAGACATAATTAGCATCAAACTTGGTGATATCGTTCTATCCGGTGCACGCCTTCTTGAAGGTGATCCTTTAAAGATTGATCAGCTTTTAACATCAATTGGATACTTTTGCATCTATTCAATTGTTGTTGGAATGTTTATTGGAATTCGGAGGAGGGAGAGTGGTGGTGCTGCTGCTGTTATTGCAAACCTACTCGTCATCCTAATTGGTGGGATCCCTATTGCTATGCCAACTGTTCTTTCCATTATGATGACCAGTGGTTCACATCGCTTGTCTCGACGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

261

Amino Acids

28.67

Weight (kDa)

9.4

Isoelectric Point (pI)

39.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E1-E2_ATPase PF00122 90 - 124 7.9e-07 P-type ATPase actuator domain
E1-E2_ATPase PF00122 144 - 194 8.9e-09 P-type ATPase actuator domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000545)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36571
pyrus_communis pycom02g01560 pycom08g10820 pycom09g05820 pycom13g21850 pycom14g16010
rosa_chinensis RchiOBHm_Chr2g0094861 RchiOBHm_Chr2g0124911 RchiOBHm_Chr2g0138581 RchiOBHm_Chr2g0160851 RchiOBHm_Chr4g0407411
rosa_laevigata RLG00000001652 RLG00000001872 RLG00000002037 RLG00000003076 RLG00000006419 RLG00000016771 RLG00000017383 RLG00000022851 RLG00000030563 RLG00000033869 RLG00000034176
rosa_multiflora Rmu_co8013424.1_g000001 Rmu_co8016716.1_g000001 Rmu_co8183472.1_g000001 Rmu_co8254047.1_g000001 Rmu_co8354891.1_g000001 Rmu_sc0000124.1_g000003 Rmu_sc0002132.1_g000027 Rmu_sc0003007.1_g000011 Rmu_sc0003221.1_g000096 Rmu_sc0003221.1_g000097 Rmu_sc0005119.1_g000004 Rmu_sc0008057.1_g000005 Rmu_sc0034532.1_g000001 Rmu_sc0036406.1_g000001 Rmu_ssc0000008.1_g000009
rosa_roxburghii Rroxscaffold_1G00030520 Rroxscaffold_2G00107360 Rroxscaffold_2G00153420 Rroxscaffold_5G00335750 Rroxscaffold_5G00351760 Rroxscaffold_5G00352080 Rroxscaffold_6G00419090
rosa_rugosa Rorug01G0341800 Rorug01G0341900 Rorug02G0180200 Rorug02G0180300 Rorug03G0186100 Rorug03G0186200 Rorug03G0284800 Rorug04G0104500 Rorug05G0246300 Rorug06G0108400 Rorug06G0471500 Rorug06G0510400
rosa_samantha Rh1AG159000 Rh1CG148200 Rh1CG189900 Rh2AG552800 Rh2BG473900 Rh2BG612100 Rh2BG612300 Rh2DG094500 Rh2DG482600 Rh3BG234000 Rh3CG320000 Rh4AG144200 Rh4BG127900 Rh4DG097300 Rh5AG128900 Rh5AG381200 Rh5BG363000 Rh5DG491900 Rh6AG048400 Rh6BG168100 Rh6CG005800 Rh6DG006000 Rh7AG255100 Rh7BG360600 Rh7CG271700 Rh7CG482300 Rh7DG451200
rosa_wichuraiana Rw4G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 60
AatII GACGTC 1 cut(s) 132
AccBSI CCGCTC 1 cut(s) 40
AciI CCGC 3 cut(s) 40, 320, 482
AclWI GGATC 3 cut(s) 557, 706, 719
AcsI RAATTY 2 cut(s) 46, 642
AcyI GRCGYC 1 cut(s) 129
AgsI TTSAA 4 cut(s) 46, 71, 557, 618
AjnI CCWGG 2 cut(s) 334, 496
AjuI GAANNNNNNNTTGG 2 cut(s) 721, 753
AluBI AGCT 3 cut(s) 22, 67, 583
AluI AGCT 3 cut(s) 22, 67, 583
Alw21I GWGCWC 2 cut(s) 24, 547
Alw26I GTCTC 4 cut(s) 136, 333, 495, 777
Alw44I GTGCAC 1 cut(s) 543
AlwI GGATC 3 cut(s) 557, 706, 719
AoxI GGCC 2 cut(s) 270, 432
ApaLI GTGCAC 1 cut(s) 543
ApeKI GCWGC 7 cut(s) 245, 248, 251, 410, 413, 668, 671
ApoI RAATTY 2 cut(s) 46, 642
AseI ATTAAT 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 271, 433
AsuHPI GGTGA 4 cut(s) 137, 374, 536, 572
BaeGI GKGCMC 1 cut(s) 547
BamHI GGATCC 1 cut(s) 711
BanII GRGCYC 1 cut(s) 24
Bbv12I GWGCWC 2 cut(s) 24, 547
BbvI GCAGC 7 cut(s) 232, 235, 238, 397, 400, 655, 658
BccI CCATC 2 cut(s) 6, 218
BciT130I CCWGG 2 cut(s) 336, 498
BciVI GTATCC 1 cut(s) 593
BclI TGATCA 1 cut(s) 577
BcoDI GTCTC 4 cut(s) 136, 333, 495, 777
BfaI CTAG 3 cut(s) 111, 324, 486
BfuI GTATCC 1 cut(s) 593
BisI GCNGC 9 cut(s) 246, 249, 252, 321, 411, 414, 483, 669, 672
BlsI GCNGC 9 cut(s) 247, 250, 253, 322, 412, 415, 484, 670, 673
Bme1390I CCNGG 2 cut(s) 336, 498
BmgT120I GGNCC 2 cut(s) 271, 433
BmiI GGNNCC 5 cut(s) 273, 333, 435, 495, 713
BmrFI CCNGG 2 cut(s) 336, 498
BmsI GCATC 7 cut(s) 78, 145, 307, 360, 469, 522, 618
BpmI CTGGAG 2 cut(s) 357, 519
BsaAI YACGTR 2 cut(s) 293, 455
BsaHI GRCGYC 1 cut(s) 129
BsaWI WCCGGW 1 cut(s) 539
BsaXI ACNNNNNCTCC 2 cut(s) 643, 673
Bse1I ACTGG 1 cut(s) 753
Bse3DI GCAATG 2 cut(s) 247, 412
BseBI CCWGG 2 cut(s) 336, 498
BseGI GGATG 2 cut(s) 186, 696
BseMI GCAATG 2 cut(s) 247, 412
BseNI ACTGG 1 cut(s) 753
BseRI GAGGAG 1 cut(s) 664
BseSI GKGCMC 1 cut(s) 547
BseXI GCAGC 7 cut(s) 232, 235, 238, 397, 400, 655, 658
Bsh1285I CGRYCG 2 cut(s) 269, 431
BshFI GGCC 2 cut(s) 272, 434
BsiEI CGRYCG 2 cut(s) 269, 431
BsiHKAI GWGCWC 2 cut(s) 24, 547
BsiSI CCGG 1 cut(s) 540
BsmAI GTCTC 4 cut(s) 136, 333, 495, 777
BsmBI CGTCTC 1 cut(s) 136
BsnI GGCC 2 cut(s) 272, 434
Bsp1286I GDGCHC 2 cut(s) 24, 547
Bsp143I GATC 3 cut(s) 562, 577, 711
BspACI CCGC 3 cut(s) 40, 320, 482
BspANI GGCC 2 cut(s) 272, 434
BspHI TCATGA 1 cut(s) 166
BspLI GGNNCC 5 cut(s) 273, 333, 435, 495, 713
BspPI GGATC 3 cut(s) 557, 706, 719
BsrBI CCGCTC 1 cut(s) 40
BsrDI GCAATG 2 cut(s) 247, 412
BsrI ACTGG 1 cut(s) 753
BssMI GATC 3 cut(s) 562, 577, 711
BssNI GRCGYC 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 336, 498
Bst4CI ACNGT 1 cut(s) 733
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 2 cut(s) 242, 407
BstBAI YACGTR 2 cut(s) 293, 455
BstC8I GCNNGC 3 cut(s) 238, 403, 547
BstF5I GGATG 2 cut(s) 186, 696
BstKTI GATC 3 cut(s) 565, 580, 714
BstMAI GTCTC 4 cut(s) 136, 333, 495, 777
BstMBI GATC 3 cut(s) 562, 577, 711
BstMCI CGRYCG 2 cut(s) 269, 431
BstNI CCWGG 2 cut(s) 336, 498
BstSCI CCNGG 2 cut(s) 334, 496
BstSLI GKGCMC 1 cut(s) 547
BstV1I GCAGC 7 cut(s) 232, 235, 238, 397, 400, 655, 658
BstX2I RGATCY 1 cut(s) 711
BstYI RGATCY 1 cut(s) 711
BsuI GTATCC 1 cut(s) 593
BsuRI GGCC 2 cut(s) 272, 434
BtgZI GCGATG 1 cut(s) 749
BtsCI GGATG 2 cut(s) 186, 696
BtsIMutI CAGTG 1 cut(s) 760
Cac8I GCNNGC 3 cut(s) 238, 403, 547
CciI TCATGA 1 cut(s) 166
Cfr13I GGNCC 2 cut(s) 271, 433
CviAII CATG 4 cut(s) 103, 122, 167, 783
CviJI RGCY 9 cut(s) 22, 67, 263, 272, 323, 425, 434, 485, 583
CviKI_1 RGCY 9 cut(s) 22, 67, 263, 272, 323, 425, 434, 485, 583
DpnI GATC 3 cut(s) 564, 579, 713
DpnII GATC 3 cut(s) 562, 577, 711
DraI TTTAAA 1 cut(s) 570
Ecl136II GAGCTC 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 24
Eco32I GATATC 2 cut(s) 367, 529
Eco53kI GAGCTC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 642
EcoRII CCWGG 2 cut(s) 334, 496
EcoRV GATATC 2 cut(s) 367, 529
EcoT38I GRGCYC 1 cut(s) 24
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 4 cut(s) 106, 125, 170, 786
FatI CATG 4 cut(s) 102, 121, 166, 782
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 9 cut(s) 246, 249, 252, 321, 411, 414, 483, 669, 672
FokI GGATG 2 cut(s) 173, 683
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 9 cut(s) 246, 249, 252, 321, 411, 414, 483, 669, 672
FspBI CTAG 3 cut(s) 111, 324, 486
GluI GCNGC 9 cut(s) 246, 249, 252, 321, 411, 414, 483, 669, 672
GsuI CTGGAG 2 cut(s) 357, 519
HaeIII GGCC 2 cut(s) 272, 434
HapII CCGG 1 cut(s) 540
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 4 cut(s) 106, 125, 170, 786
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 1 cut(s) 106
HpaII CCGG 1 cut(s) 540
HphI GGTGA 4 cut(s) 137, 374, 536, 572
Hpy166II GTNNAC 2 cut(s) 545, 761
Hpy188I TCNGA 1 cut(s) 648
Hpy188III TCNNGA 4 cut(s) 85, 167, 554, 774
Hpy8I GTNNAC 2 cut(s) 545, 761
Hpy99I CGWCG 1 cut(s) 780
HpyAV CCTTC 5 cut(s) 73, 277, 439, 551, 560
HpyCH4III ACNGT 1 cut(s) 733
HpyCH4IV ACGT 3 cut(s) 129, 292, 454
HpyCH4V TGCA 9 cut(s) 4, 91, 145, 158, 236, 401, 545, 609, 683
HpySE526I ACGT 3 cut(s) 129, 292, 454
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 4 cut(s) 106, 125, 170, 786
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 3 cut(s) 562, 577, 711
LpnPI CCDG 8 cut(s) 222, 321, 348, 387, 483, 510, 553, 766
Lsp1109I GCAGC 7 cut(s) 232, 235, 238, 397, 400, 655, 658
LweI GCATC 7 cut(s) 78, 145, 307, 360, 469, 522, 618
MaeI CTAG 3 cut(s) 111, 324, 486
MaeII ACGT 3 cut(s) 129, 292, 454
MaeIII GTNAC 3 cut(s) 125, 293, 455
MalI GATC 3 cut(s) 564, 579, 713
MbiI CCGCTC 1 cut(s) 40
MboI GATC 3 cut(s) 562, 577, 711
MboII GAAGA 1 cut(s) 83
MfeI CAATTG 2 cut(s) 594, 618
MflI RGATCY 1 cut(s) 711
MhlI GDGCHC 2 cut(s) 24, 547
MluCI AATT 8 cut(s) 46, 202, 345, 507, 594, 618, 642, 702
MmeI TCCRAC 1 cut(s) 607
MnlI CCTC 4 cut(s) 217, 382, 642, 645
MseI TTAA 5 cut(s) 33, 98, 201, 569, 587
MslI CAYNNNNRTG 2 cut(s) 150, 746
MspI CCGG 1 cut(s) 540
MspR9I CCNGG 2 cut(s) 336, 498
MunI CAATTG 2 cut(s) 594, 618
MvaI CCWGG 2 cut(s) 336, 498
NdeII GATC 3 cut(s) 562, 577, 711
NlaIII CATG 4 cut(s) 106, 125, 170, 786
NlaIV GGNNCC 5 cut(s) 273, 333, 435, 495, 713
NmuCI GTSAC 3 cut(s) 125, 293, 455
PagI TCATGA 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 772
PfeI GAWTC 1 cut(s) 106
PfoI TCCNGGA 2 cut(s) 334, 496
PkrI GCNGC 9 cut(s) 247, 250, 253, 322, 412, 415, 484, 670, 673
Ppu21I YACGTR 2 cut(s) 293, 455
PshBI ATTAAT 1 cut(s) 201
PsiI TTATAA 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 334, 496
PspGI CCWGG 2 cut(s) 334, 496
PspN4I GGNNCC 5 cut(s) 273, 333, 435, 495, 713
PspPI GGNCC 2 cut(s) 271, 433
PsuI RGATCY 1 cut(s) 711
RseI CAYNNNNRTG 2 cut(s) 150, 746
SacI GAGCTC 1 cut(s) 24
SaqAI TTAA 5 cut(s) 33, 98, 201, 569, 587
SatI GCNGC 9 cut(s) 246, 249, 252, 321, 411, 414, 483, 669, 672
Sau3AI GATC 3 cut(s) 562, 577, 711
Sau96I GGNCC 2 cut(s) 271, 433
ScrFI CCNGG 2 cut(s) 336, 498
SduI GDGCHC 2 cut(s) 24, 547
SfaNI GCATC 7 cut(s) 78, 145, 307, 360, 469, 522, 618
SmiMI CAYNNNNRTG 2 cut(s) 150, 746
Sse9I AATT 8 cut(s) 46, 202, 345, 507, 594, 618, 642, 702
SsiI CCGC 3 cut(s) 40, 320, 482
SspMI CTAG 3 cut(s) 111, 324, 486
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 2 cut(s) 334, 496
TaaI ACNGT 1 cut(s) 733
TaiI ACGT 3 cut(s) 132, 295, 457
TaqI TCGA 2 cut(s) 24, 775
TaqII GACCGA 2 cut(s) 255, 417
TasI AATT 8 cut(s) 46, 202, 345, 507, 594, 618, 642, 702
TauI GCSGC 2 cut(s) 323, 485
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 5 cut(s) 33, 98, 201, 569, 587
Tru9I TTAA 5 cut(s) 33, 98, 201, 569, 587
TscAI CASTG 1 cut(s) 760
TseFI GTSAC 3 cut(s) 125, 293, 455
TseI GCWGC 7 cut(s) 245, 248, 251, 410, 413, 668, 671
Tsp45I GTSAC 3 cut(s) 125, 293, 455
TspDTI ATGAA 2 cut(s) 42, 119
TspGWI ACGGA 2 cut(s) 312, 474
TspRI CASTG 1 cut(s) 760
VneI GTGCAC 1 cut(s) 543
VspI ATTAAT 1 cut(s) 201
XapI RAATTY 2 cut(s) 46, 642
XspI CTAG 3 cut(s) 111, 324, 486
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.