Rorug03G0284800

ATPase 11, plasma

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000545
Physical Location & Seq
Reverse (-)
28621471 .. 28625618
4148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0284800.1

Sequence Viewer

Length: 1401 bp
ATGAGGAGGAGATCTTCTACTCTGCCTGTGGTGGATGTGTTTGTGGTACTTTTTGCATTGTTCACCGGTGCTCCGTTTCCATGGGCTGCTGTCAAGGGCAGTCATCCACATTGCAAGAAATTAAGGGAAAACATGGGCGAAATTCGACCTCACAGTGTCACCATCACTGAATTTGGCGCAGTTGGTGATGGGGTCACTCTCAACACCAAAGCCTTTCAGAATGCCATCTTCTATCTCAACTCTTTTTCTGACAAGGGTGGGGCCAAGCTTTTTGTCCCCGCTGGCCGGTGGTTGACAGGAAGCTTCCATCTCATCAGTCATCTAACCTTGTGGTTGGATGAAGATGCAGTCATTCTTGGATCAATGAACTCAGATGATTGGCCAGTTGTTGATCCCTTGCCATCATATGGTCGAGGAAGGGAGTTGATGGGGGAAAGACATCAAAGCCTCATCTATGGGCACAATTTGACCGATGTTATCATAACTGGTCATAATGGAACGATTGATGGTCAAGGCAGCATTTGGTGGAATTCGTTCCGTAACAAAACTCTGAATTATACAAGGCCCCATTTGGTGGAGTTGATGAACTCAACTGGGGTTGTCATCTCAAACCTGACTTTCTTAAATTCACCATTTTGGACCATTCACCCAGTATACTGCAGCCATGTTTCCGTCCAGAATCTCACAATCCTTGCTCCTCTCAATTCACCAAACACGGATGGGATCGATCCAGACTCTTCTGATAATGTCTGCATTGAAGACTGTTATATAAGCACCGGTGATGATCTGATCTCCATCAAAAGTGGGTGGGATGAGTATGGCATTTCATATGGTCGTCCCAGTAGAAACATTCTCATTCGCGGGATTACGGGGAAAACTCAAACAAGTGCAGGAATTTCAATTGGAAGTGAGATGTCTGGAGGTGTTTCAGAAGTTCATGCAGAAAATCTCCATTTTTTCGGTTCTCATACAGGTATCAGAATAAAGACATCTCCGGGAAGGGGTGGTTATGTCAGAAATATCTATGTGTCAAATATGACCTTGGATGGTGTATATATAGCTATAAGGTTCACCAGTCACTATGGAGAACACCCAGATGAGTTTTTCGACCCAAGTGCCCTCCCTATCGTAGAACAGATTACTTTTAAAGATGTCATAGGCGATAATATCAAAACTGCAGGCCTCCTAGAGGGTTTAGAAGGTGGCAGTTTTCAGAACATTTGCCTATCCAATATTATCCTCAATGTAACATCAAAGTCCCCATGGAACTGCTCTTCTATTCATGGATATTCTTACTTTGTTTCTCCAGAAATCTGTCAGCCTCTGAAGGAGAGTATCTCCCCTGAGCATTACTCGGATTGTTACCATCTATCAGATCAGAAATGGTTTTCCAGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

466

Amino Acids

51.39

Weight (kDa)

5.85

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 148 - 428 5.9e-43 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000545)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36571
pyrus_communis pycom02g01560 pycom08g10820 pycom09g05820 pycom13g21850 pycom14g16010
rosa_chinensis RchiOBHm_Chr2g0094861 RchiOBHm_Chr2g0124911 RchiOBHm_Chr2g0138581 RchiOBHm_Chr2g0160851 RchiOBHm_Chr4g0407411
rosa_laevigata RLG00000001652 RLG00000001872 RLG00000002037 RLG00000003076 RLG00000006419 RLG00000016771 RLG00000017383 RLG00000022851 RLG00000030563 RLG00000033869 RLG00000034176
rosa_multiflora Rmu_co8013424.1_g000001 Rmu_co8016716.1_g000001 Rmu_co8183472.1_g000001 Rmu_co8254047.1_g000001 Rmu_co8354891.1_g000001 Rmu_sc0000124.1_g000003 Rmu_sc0002132.1_g000027 Rmu_sc0003007.1_g000011 Rmu_sc0003221.1_g000096 Rmu_sc0003221.1_g000097 Rmu_sc0005119.1_g000004 Rmu_sc0008057.1_g000005 Rmu_sc0034532.1_g000001 Rmu_sc0036406.1_g000001 Rmu_ssc0000008.1_g000009
rosa_roxburghii Rroxscaffold_1G00030520 Rroxscaffold_2G00107360 Rroxscaffold_2G00153420 Rroxscaffold_5G00335750 Rroxscaffold_5G00351760 Rroxscaffold_5G00352080 Rroxscaffold_6G00419090
rosa_rugosa Rorug01G0341800 Rorug01G0341900 Rorug02G0180200 Rorug02G0180300 Rorug03G0186100 Rorug03G0186200 Rorug03G0284800 Rorug04G0104500 Rorug05G0246300 Rorug06G0108400 Rorug06G0471500 Rorug06G0510400
rosa_samantha Rh1AG159000 Rh1CG148200 Rh1CG189900 Rh2AG552800 Rh2BG473900 Rh2BG612100 Rh2BG612300 Rh2DG094500 Rh2DG482600 Rh3BG234000 Rh3CG320000 Rh4AG144200 Rh4BG127900 Rh4DG097300 Rh5AG128900 Rh5AG381200 Rh5BG363000 Rh5DG491900 Rh6AG048400 Rh6BG168100 Rh6CG005800 Rh6DG006000 Rh7AG255100 Rh7BG360600 Rh7CG271700 Rh7CG482300 Rh7DG451200
rosa_wichuraiana Rw4G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 407, 574
AccI GTMKAC 1 cut(s) 654
AccII CGCG 1 cut(s) 861
AciI CCGC 2 cut(s) 279, 861
AclWI GGATC 4 cut(s) 367, 386, 722, 731
AcoI YGGCCR 2 cut(s) 283, 380
AcsI RAATTY 5 cut(s) 141, 170, 529, 625, 894
AcuI CTGAAG 1 cut(s) 1346
AfaI GTAC 1 cut(s) 48
AfiI CCNNNNNNNGG 5 cut(s) 285, 407, 574, 1001, 1189
AgeI ACCGGT 2 cut(s) 65, 776
AgsI TTSAA 2 cut(s) 758, 900
AluBI AGCT 3 cut(s) 268, 303, 1061
AluI AGCT 3 cut(s) 268, 303, 1061
Alw21I GWGCWC 1 cut(s) 73
AlwI GGATC 4 cut(s) 367, 386, 722, 731
AlwNI CAGNNNCTG 1 cut(s) 1324
AoxI GGCC 5 cut(s) 261, 283, 380, 563, 1180
ApeKI GCWGC 3 cut(s) 86, 516, 660
ApoI RAATTY 5 cut(s) 141, 170, 529, 625, 894
AsiGI ACCGGT 2 cut(s) 65, 776
Asp700I GAANNNNTTC 1 cut(s) 533
AspLEI GCGC 1 cut(s) 179
AspS9I GGNCC 3 cut(s) 261, 564, 639
AsuC2I CCSGG 1 cut(s) 996
AsuHPI GGTGA 8 cut(s) 55, 151, 197, 621, 638, 699, 791, 1063
AvaII GGWCC 1 cut(s) 639
BaeGI GKGCMC 2 cut(s) 462, 1120
BalI TGGCCA 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 765
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 3 cut(s) 73, 528, 672
BcgI CGANNNNNNTGC 2 cut(s) 1097, 1131
BcnI CCSGG 1 cut(s) 996
BfaI CTAG 1 cut(s) 1187
BfmI CTRYAG 2 cut(s) 658, 1176
BglII AGATCT 1 cut(s) 11
BisI GCNGC 3 cut(s) 87, 517, 661
BlsI GCNGC 3 cut(s) 88, 518, 662
Bme1390I CCNGG 1 cut(s) 996
Bme18I GGWCC 1 cut(s) 639
BmgT120I GGNCC 3 cut(s) 261, 564, 639
BmiI GGNNCC 2 cut(s) 262, 566
BmrFI CCNGG 1 cut(s) 996
BmrI ACTGGG 3 cut(s) 603, 644, 834
BmsI GCATC 1 cut(s) 334
BmuI ACTGGG 3 cut(s) 603, 644, 834
BpiI GAAGAC 1 cut(s) 765
BplI GAGNNNNNCTC 4 cut(s) 1322, 1354, 1337, 1369
BpmI CTGGAG 2 cut(s) 939, 1290
Bpu10I CCTNAGC 1 cut(s) 1344
BpuMI CCSGG 1 cut(s) 996
Bsa29I ATCGAT 1 cut(s) 726
BsaBI GATNNNNATC 1 cut(s) 794
BsaJI CCNNGG 3 cut(s) 80, 1041, 1262
BsaWI WCCGGW 2 cut(s) 65, 776
BsaXI ACNNNNNCTCC 2 cut(s) 55, 85
Bsc4I CCNNNNNNNGG 5 cut(s) 285, 407, 574, 1001, 1189
Bse118I RCCGGY 3 cut(s) 65, 285, 776
Bse1I ACTGG 7 cut(s) 383, 490, 598, 650, 840, 1074, 1392
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 794
BseCI ATCGAT 1 cut(s) 726
BseDI CCNNGG 3 cut(s) 80, 1041, 1262
BseGI GGATG 6 cut(s) 40, 103, 343, 724, 817, 1051
BseJI GATNNNNATC 1 cut(s) 794
BseLI CCNNNNNNNGG 5 cut(s) 285, 407, 574, 1001, 1189
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 384, 1335
BseNI ACTGG 7 cut(s) 383, 490, 598, 650, 840, 1074, 1392
BseRI GAGGAG 3 cut(s) 19, 22, 687
BseSI GKGCMC 2 cut(s) 462, 1120
BseXI GCAGC 3 cut(s) 73, 528, 672
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 1 cut(s) 861
BshFI GGCC 5 cut(s) 263, 285, 382, 565, 1182
BshTI ACCGGT 2 cut(s) 65, 776
BshVI ATCGAT 1 cut(s) 726
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 4 cut(s) 66, 286, 777, 995
BslFI GGGAC 3 cut(s) 260, 822, 1243
BslI CCNNNNNNNGG 5 cut(s) 285, 407, 574, 1001, 1189
BsmFI GGGAC 3 cut(s) 260, 822, 1243
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 5 cut(s) 263, 285, 382, 565, 1182
Bsp1286I GDGCHC 3 cut(s) 73, 462, 1120
Bsp143I GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
Bsp19I CCATGG 2 cut(s) 80, 1262
BspACI CCGC 2 cut(s) 279, 861
BspANI GGCC 5 cut(s) 263, 285, 382, 565, 1182
BspCNI CTCAG 2 cut(s) 383, 1336
BspDI ATCGAT 1 cut(s) 726
BspFNI CGCG 1 cut(s) 861
BspLI GGNNCC 2 cut(s) 262, 566
BspMAI CTGCAG 2 cut(s) 662, 1180
BspPI GGATC 4 cut(s) 367, 386, 722, 731
BspQI GCTCTTC 1 cut(s) 1279
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 3 cut(s) 65, 285, 776
BsrI ACTGG 7 cut(s) 383, 490, 598, 650, 840, 1074, 1392
BssAI RCCGGY 3 cut(s) 65, 285, 776
BssECI CCNNGG 3 cut(s) 80, 1041, 1262
BssMI GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
BssNAI GTATAC 1 cut(s) 655
BssT1I CCWWGG 3 cut(s) 80, 1041, 1262
Bst1107I GTATAC 1 cut(s) 655
Bst4CI ACNGT 2 cut(s) 155, 764
Bst6I CTCTTC 2 cut(s) 742, 1279
BstC8I GCNNGC 2 cut(s) 283, 1180
BstDEI CTNAG 2 cut(s) 370, 1344
BstDSI CCRYGG 2 cut(s) 80, 1262
BstENI CCTNNNNNAGG 1 cut(s) 1187
BstF5I GGATG 6 cut(s) 40, 103, 343, 724, 817, 1051
BstFNI CGCG 1 cut(s) 861
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 8 cut(s) 14, 362, 394, 726, 730, 787, 792, 1378
BstMBI GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
BstSCI CCNGG 1 cut(s) 994
BstSFI CTRYAG 2 cut(s) 658, 1176
BstSLI GKGCMC 2 cut(s) 462, 1120
BstUI CGCG 1 cut(s) 861
BstV1I GCAGC 3 cut(s) 73, 528, 672
BstV2I GAAGAC 1 cut(s) 765
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
BstZ17I GTATAC 1 cut(s) 655
Bsu15I ATCGAT 1 cut(s) 726
BsuRI GGCC 5 cut(s) 263, 285, 382, 565, 1182
BsuTUI ATCGAT 1 cut(s) 726
BtgI CCRYGG 2 cut(s) 80, 1262
BtsCI GGATG 6 cut(s) 40, 103, 343, 724, 817, 1051
BtsIMutI CAGTG 3 cut(s) 160, 165, 1399
Cac8I GCNNGC 2 cut(s) 283, 1180
CaiI CAGNNNCTG 1 cut(s) 1324
CfoI GCGC 1 cut(s) 179
Cfr10I RCCGGY 3 cut(s) 65, 285, 776
Cfr13I GGNCC 3 cut(s) 261, 564, 639
ClaI ATCGAT 1 cut(s) 726
Csp6I GTAC 1 cut(s) 47
CspAI ACCGGT 2 cut(s) 65, 776
CviAII CATG 6 cut(s) 81, 133, 665, 938, 1263, 1283
CviQI GTAC 1 cut(s) 47
DdeI CTNAG 2 cut(s) 370, 1344
DpnI GATC 8 cut(s) 13, 361, 393, 725, 729, 786, 791, 1377
DpnII GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
DraI TTTAAA 1 cut(s) 1147
EaeI YGGCCR 2 cut(s) 283, 380
Eam1104I CTCTTC 2 cut(s) 742, 1279
EarI CTCTTC 2 cut(s) 742, 1279
Eco130I CCWWGG 3 cut(s) 80, 1041, 1262
Eco147I AGGCCT 1 cut(s) 1182
Eco47I GGWCC 1 cut(s) 639
Eco57I CTGAAG 1 cut(s) 1346
EcoNI CCTNNNNNAGG 1 cut(s) 1187
EcoO109I RGGNCCY 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 529
EcoT14I CCWWGG 3 cut(s) 80, 1041, 1262
ErhI CCWWGG 3 cut(s) 80, 1041, 1262
FaeI CATG 6 cut(s) 84, 136, 668, 941, 1266, 1286
FaqI GGGAC 3 cut(s) 260, 822, 1243
FatI CATG 6 cut(s) 80, 132, 664, 937, 1262, 1282
FauI CCCGC 2 cut(s) 286, 854
FauNDI CATATG 2 cut(s) 406, 829
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 3 cut(s) 87, 517, 661
FokI GGATG 6 cut(s) 47, 90, 350, 731, 824, 1058
Fsp4HI GCNGC 3 cut(s) 87, 517, 661
FspBI CTAG 1 cut(s) 1187
GlaI GCGC 1 cut(s) 178
GluI GCNGC 3 cut(s) 87, 517, 661
GsuI CTGGAG 2 cut(s) 939, 1290
HaeIII GGCC 5 cut(s) 263, 285, 382, 565, 1182
HapII CCGG 4 cut(s) 66, 286, 777, 995
HhaI GCGC 1 cut(s) 179
Hin1II CATG 6 cut(s) 84, 136, 668, 941, 1266, 1286
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HindIII AAGCTT 2 cut(s) 266, 301
HinfI GANTC 2 cut(s) 679, 734
HpaII CCGG 4 cut(s) 66, 286, 777, 995
HphI GGTGA 8 cut(s) 55, 151, 197, 621, 638, 699, 791, 1063
Hpy166II GTNNAC 4 cut(s) 63, 294, 655, 1071
Hpy188III TCNNGA 4 cut(s) 676, 731, 918, 1307
Hpy8I GTNNAC 4 cut(s) 63, 294, 655, 1071
HpyAV CCTTC 4 cut(s) 411, 993, 1193, 1321
HpyCH4III ACNGT 2 cut(s) 155, 764
HpyCH4V TGCA 9 cut(s) 56, 114, 347, 660, 753, 890, 941, 1178, 1397
HpyF3I CTNAG 2 cut(s) 370, 1344
Hsp92II CATG 6 cut(s) 84, 136, 668, 941, 1266, 1286
HspAI GCGC 1 cut(s) 177
Kzo9I GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
LguI GCTCTTC 1 cut(s) 1279
LmnI GCTCC 2 cut(s) 76, 700
Lsp1109I GCAGC 3 cut(s) 73, 528, 672
LweI GCATC 1 cut(s) 334
MaeI CTAG 1 cut(s) 1187
MaeIII GTNAC 6 cut(s) 157, 193, 539, 1076, 1246, 1361
MalI GATC 8 cut(s) 13, 361, 393, 725, 729, 786, 791, 1377
MboI GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
MboII GAAGA 6 cut(s) 6, 220, 353, 729, 770, 1266
MfeI CAATTG 1 cut(s) 900
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 3 cut(s) 73, 462, 1120
MlsI TGGCCA 1 cut(s) 382
MluNI TGGCCA 1 cut(s) 382
MlyI GAGTC 1 cut(s) 728
MmeI TCCRAC 1 cut(s) 315
Mox20I TGGCCA 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 533
MscI TGGCCA 1 cut(s) 382
MseI TTAA 3 cut(s) 122, 623, 1146
MslI CAYNNNNRTG 1 cut(s) 1095
Msp20I TGGCCA 1 cut(s) 382
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 4 cut(s) 66, 286, 777, 995
MspR9I CCNGG 1 cut(s) 996
MunI CAATTG 1 cut(s) 900
Mva1269I GAATGC 1 cut(s) 226
MvnI CGCG 1 cut(s) 861
NciI CCSGG 1 cut(s) 996
NcoI CCATGG 2 cut(s) 80, 1262
NdeI CATATG 2 cut(s) 406, 829
NdeII GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
NlaIII CATG 6 cut(s) 84, 136, 668, 941, 1266, 1286
NlaIV GGNNCC 2 cut(s) 262, 566
NmuCI GTSAC 3 cut(s) 157, 193, 1076
PceI AGGCCT 1 cut(s) 1182
PciSI GCTCTTC 1 cut(s) 1279
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 533
PfeI GAWTC 1 cut(s) 679
PflMI CCANNNNNTGG 2 cut(s) 407, 574
PfoI TCCNGGA 1 cut(s) 994
PinAI ACCGGT 2 cut(s) 65, 776
PkrI GCNGC 3 cut(s) 88, 518, 662
PleI GAGTC 1 cut(s) 728
PpsI GAGTC 1 cut(s) 728
PspN4I GGNNCC 2 cut(s) 262, 566
PspPI GGNCC 3 cut(s) 261, 564, 639
PstI CTGCAG 2 cut(s) 662, 1180
PstNI CAGNNNCTG 1 cut(s) 1324
PsuI RGATCY 1 cut(s) 11
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
RseI CAYNNNNRTG 1 cut(s) 1095
SapI GCTCTTC 1 cut(s) 1279
SaqAI TTAA 3 cut(s) 122, 623, 1146
SatI GCNGC 3 cut(s) 87, 517, 661
Sau3AI GATC 8 cut(s) 11, 359, 391, 723, 727, 784, 789, 1375
Sau96I GGNCC 3 cut(s) 261, 564, 639
SchI GAGTC 1 cut(s) 728
ScrFI CCNGG 1 cut(s) 996
SduI GDGCHC 3 cut(s) 73, 462, 1120
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 2 cut(s) 658, 1176
SgrAI CRCCGGYG 2 cut(s) 65, 776
SinI GGWCC 1 cut(s) 639
SmiMI CAYNNNNRTG 1 cut(s) 1095
SseBI AGGCCT 1 cut(s) 1182
SsiI CCGC 2 cut(s) 279, 861
SspI AATATT 1 cut(s) 1234
SspMI CTAG 1 cut(s) 1187
StuI AGGCCT 1 cut(s) 1182
StyD4I CCNGG 1 cut(s) 994
StyI CCWWGG 3 cut(s) 80, 1041, 1262
TaaI ACNGT 2 cut(s) 155, 764
TaqI TCGA 4 cut(s) 145, 412, 726, 1107
TaqII GACCGA 1 cut(s) 485
TfiI GAWTC 1 cut(s) 679
Tru1I TTAA 3 cut(s) 122, 623, 1146
Tru9I TTAA 3 cut(s) 122, 623, 1146
TscAI CASTG 3 cut(s) 160, 172, 1399
TseFI GTSAC 3 cut(s) 157, 193, 1076
TseI GCWGC 3 cut(s) 86, 516, 660
Tsp45I GTSAC 3 cut(s) 157, 193, 1076
TspDTI ATGAA 6 cut(s) 354, 380, 599, 816, 926, 1271
TspGWI ACGGA 4 cut(s) 63, 527, 661, 731
TspRI CASTG 3 cut(s) 160, 172, 1399
Van91I CCANNNNNTGG 2 cut(s) 407, 574
VpaK11BI GGWCC 1 cut(s) 639
XagI CCTNNNNNAGG 1 cut(s) 1187
XapI RAATTY 5 cut(s) 141, 170, 529, 625, 894
XmiI GTMKAC 1 cut(s) 654
XmnI GAANNNNTTC 1 cut(s) 533
XspI CTAG 1 cut(s) 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.