Rorug02G0180200

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000545
Physical Location & Seq
Forward (+)
16706602 .. 16707251
650 bp
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UTR
Exon/CDS
Intron
Rorug02G0180200.1

Sequence Viewer

Length: 567 bp
ATGCGCACTGCACTTATAACCGACTTGCTTTTACTTGAAAACCACCTTCCTTGGAGAGTTTTTAACTGTTTGCTTCACCTCACCTCCGAAGCTGATAAGTTTCCTCTTCCTAAGCTTACTCTAAAGTACTTTGAGGGGTACACAATGGGGACATATCCACAGGCTAATGCAGCATTAGAAAATAAACATTTGCTGGACTTTTTAGGTAATTGTTTACAAGGATCTTCTGCTGAATCTGCAGTGTTTGAGAACTATAGCCAGAGGCGCCCCTCGATCCCCTCTGTGACAGAGCTTGATTATTTTGGTATTCGGTTTAAGCTTGGAGGCAGTAATGACGTGTGCAATGGAACATTCAGGAATGGATTTCTGACGATTCCACCATTATCAGTTCAAGAGAATGGAGAATCTCTCTTTAGAAACCTCAGAGCCTATGCACAGTTGGAACCAAGTAATAAGAGTAAATTGATCTCTTATTTTGTGTTGTTAGATGACCTTATTAAGTGCGAAAAAGATCTGGCGTTACTTAATGAAAAGGAAATTATTGCAAACTTCCGGCATGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

188

Amino Acids

21.41

Weight (kDa)

6.09

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 4 - 184 2.7e-35 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000545)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36571
pyrus_communis pycom02g01560 pycom08g10820 pycom09g05820 pycom13g21850 pycom14g16010
rosa_chinensis RchiOBHm_Chr2g0094861 RchiOBHm_Chr2g0124911 RchiOBHm_Chr2g0138581 RchiOBHm_Chr2g0160851 RchiOBHm_Chr4g0407411
rosa_laevigata RLG00000001652 RLG00000001872 RLG00000002037 RLG00000003076 RLG00000006419 RLG00000016771 RLG00000017383 RLG00000022851 RLG00000030563 RLG00000033869 RLG00000034176
rosa_multiflora Rmu_co8013424.1_g000001 Rmu_co8016716.1_g000001 Rmu_co8183472.1_g000001 Rmu_co8254047.1_g000001 Rmu_co8354891.1_g000001 Rmu_sc0000124.1_g000003 Rmu_sc0002132.1_g000027 Rmu_sc0003007.1_g000011 Rmu_sc0003221.1_g000096 Rmu_sc0003221.1_g000097 Rmu_sc0005119.1_g000004 Rmu_sc0008057.1_g000005 Rmu_sc0034532.1_g000001 Rmu_sc0036406.1_g000001 Rmu_ssc0000008.1_g000009
rosa_roxburghii Rroxscaffold_1G00030520 Rroxscaffold_2G00107360 Rroxscaffold_2G00153420 Rroxscaffold_5G00335750 Rroxscaffold_5G00351760 Rroxscaffold_5G00352080 Rroxscaffold_6G00419090
rosa_rugosa Rorug01G0341800 Rorug01G0341900 Rorug02G0180200 Rorug02G0180300 Rorug03G0186100 Rorug03G0186200 Rorug03G0284800 Rorug04G0104500 Rorug05G0246300 Rorug06G0108400 Rorug06G0471500 Rorug06G0510400
rosa_samantha Rh1AG159000 Rh1CG148200 Rh1CG189900 Rh2AG552800 Rh2BG473900 Rh2BG612100 Rh2BG612300 Rh2DG094500 Rh2DG482600 Rh3BG234000 Rh3CG320000 Rh4AG144200 Rh4BG127900 Rh4DG097300 Rh5AG128900 Rh5AG381200 Rh5BG363000 Rh5DG491900 Rh6AG048400 Rh6BG168100 Rh6CG005800 Rh6DG006000 Rh7AG255100 Rh7BG360600 Rh7CG271700 Rh7CG482300 Rh7DG451200
rosa_wichuraiana Rw4G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 264
AclWI GGATC 2 cut(s) 229, 268
AcyI GRCGYC 1 cut(s) 265
AfaI GTAC 2 cut(s) 128, 140
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 2 cut(s) 38, 392
AjiI CACGTC 1 cut(s) 337
AluBI AGCT 4 cut(s) 92, 115, 292, 319
AluI AGCT 4 cut(s) 92, 115, 292, 319
AlwI GGATC 2 cut(s) 229, 268
ApeKI GCWGC 1 cut(s) 170
AspLEI GCGC 2 cut(s) 6, 267
AsuHPI GGTGA 2 cut(s) 68, 73
BanI GGYRCC 1 cut(s) 264
BbvI GCAGC 1 cut(s) 182
BfmI CTRYAG 2 cut(s) 237, 253
BfoI RGCGCY 1 cut(s) 268
BglII AGATCT 1 cut(s) 511
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmcAI AGTACT 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 337
BmiI GGNNCC 2 cut(s) 266, 444
BplI GAGNNNNNCTC 2 cut(s) 393, 425
Bpu10I CCTNAGC 1 cut(s) 111
Bsa29I ATCGAT 1 cut(s) 562
BsaHI GRCGYC 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse3DI GCAATG 1 cut(s) 349
BseCI ATCGAT 1 cut(s) 562
BseDI CCNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 182
BshNI GGYRCC 1 cut(s) 264
BshVI ATCGAT 1 cut(s) 562
BsiSI CCGG 1 cut(s) 553
BslFI GGGAC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 163
Bsp143I GATC 5 cut(s) 221, 273, 465, 511, 559
BspCNI CTCAG 1 cut(s) 435
BspDI ATCGAT 1 cut(s) 562
BspLI GGNNCC 2 cut(s) 266, 444
BspMAI CTGCAG 1 cut(s) 241
BspPI GGATC 2 cut(s) 229, 268
BspT107I GGYRCC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 349
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 5 cut(s) 221, 273, 465, 511, 559
BssNI GRCGYC 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 68, 438
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 265
BstDEI CTNAG 2 cut(s) 111, 422
BstH2I RGCGCY 1 cut(s) 268
BstHHI GCGC 2 cut(s) 6, 267
BstKTI GATC 5 cut(s) 224, 276, 468, 514, 562
BstMBI GATC 5 cut(s) 221, 273, 465, 511, 559
BstMWI GCNNNNNNNGC 3 cut(s) 170, 236, 264
BstSFI CTRYAG 2 cut(s) 237, 253
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 2 cut(s) 221, 511
BstYI RGATCY 2 cut(s) 221, 511
Bsu15I ATCGAT 1 cut(s) 562
BsuTUI ATCGAT 1 cut(s) 562
BtrI CACGTC 1 cut(s) 337
BtsI GCAGTG 2 cut(s) 6, 246
BtsIMutI CAGTG 2 cut(s) 6, 246
CfoI GCGC 2 cut(s) 6, 267
ClaI ATCGAT 1 cut(s) 562
Csp6I GTAC 2 cut(s) 127, 139
CspCI CAANNNNNGTGG 2 cut(s) 32, 67
CviAII CATG 1 cut(s) 557
CviJI RGCY 7 cut(s) 92, 115, 164, 258, 292, 319, 428
CviKI_1 RGCY 7 cut(s) 92, 115, 164, 258, 292, 319, 428
CviQI GTAC 2 cut(s) 127, 139
DdeI CTNAG 2 cut(s) 111, 422
DinI GGCGCC 1 cut(s) 266
DpnI GATC 5 cut(s) 223, 275, 467, 513, 561
DpnII GATC 5 cut(s) 221, 273, 465, 511, 559
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 50
EcoT14I CCWWGG 1 cut(s) 50
EgeI GGCGCC 1 cut(s) 266
EheI GGCGCC 1 cut(s) 266
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 1 cut(s) 560
FaiI YATR 5 cut(s) 17, 154, 255, 432, 558
FaqI GGGAC 1 cut(s) 163
FatI CATG 1 cut(s) 556
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
FspAI RTGCGCAY 1 cut(s) 5
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 2 cut(s) 5, 266
GluI GCNGC 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 268
HapII CCGG 1 cut(s) 553
HhaI GCGC 2 cut(s) 6, 267
Hin1I GRCGYC 1 cut(s) 265
Hin1II CATG 1 cut(s) 560
Hin6I GCGC 2 cut(s) 4, 265
HinP1I GCGC 2 cut(s) 4, 265
HindIII AAGCTT 2 cut(s) 113, 317
HinfI GANTC 3 cut(s) 233, 373, 404
HpaII CCGG 1 cut(s) 553
HphI GGTGA 2 cut(s) 68, 73
Hpy166II GTNNAC 2 cut(s) 141, 215
Hpy188I TCNGA 3 cut(s) 88, 369, 425
Hpy188III TCNNGA 2 cut(s) 355, 392
Hpy8I GTNNAC 2 cut(s) 141, 215
HpyAV CCTTC 1 cut(s) 56
HpyCH4III ACNGT 2 cut(s) 68, 438
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 6 cut(s) 11, 170, 239, 342, 434, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 236, 264
HpyF3I CTNAG 2 cut(s) 111, 422
HpySE526I ACGT 1 cut(s) 336
Hsp92I GRCGYC 1 cut(s) 265
Hsp92II CATG 1 cut(s) 560
HspAI GCGC 2 cut(s) 4, 265
KasI GGCGCC 1 cut(s) 264
Kzo9I GATC 5 cut(s) 221, 273, 465, 511, 559
LpnPI CCDG 5 cut(s) 146, 179, 272, 340, 500
Lsp1109I GCAGC 1 cut(s) 182
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 283, 519
MalI GATC 5 cut(s) 223, 275, 467, 513, 561
MboI GATC 5 cut(s) 221, 273, 465, 511, 559
MboII GAAGA 2 cut(s) 98, 216
MflI RGATCY 2 cut(s) 221, 511
MluCI AATT 3 cut(s) 208, 461, 537
Mly113I GGCGCC 1 cut(s) 265
MmeI TCCRAC 1 cut(s) 420
MnlI CCTC 9 cut(s) 89, 94, 114, 127, 255, 280, 289, 317, 431
MseI TTAA 4 cut(s) 63, 315, 498, 525
MspI CCGG 1 cut(s) 553
MwoI GCNNNNNNNGC 3 cut(s) 170, 236, 264
NarI GGCGCC 1 cut(s) 265
NdeII GATC 5 cut(s) 221, 273, 465, 511, 559
NlaIII CATG 1 cut(s) 560
NlaIV GGNNCC 2 cut(s) 266, 444
NmuCI GTSAC 1 cut(s) 283
NsbI TGCGCA 1 cut(s) 5
PfeI GAWTC 3 cut(s) 233, 373, 404
PkrI GCNGC 1 cut(s) 172
PluTI GGCGCC 1 cut(s) 268
PsiI TTATAA 1 cut(s) 17
PspN4I GGNNCC 2 cut(s) 266, 444
PstI CTGCAG 1 cut(s) 241
PsuI RGATCY 2 cut(s) 221, 511
RsaI GTAC 2 cut(s) 128, 140
RsaNI GTAC 2 cut(s) 127, 139
SaqAI TTAA 4 cut(s) 63, 315, 498, 525
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 5 cut(s) 221, 273, 465, 511, 559
ScaI AGTACT 1 cut(s) 128
SfcI CTRYAG 2 cut(s) 237, 253
SfoI GGCGCC 1 cut(s) 266
Sse9I AATT 3 cut(s) 208, 461, 537
SspDI GGCGCC 1 cut(s) 264
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 68, 438
TaiI ACGT 1 cut(s) 339
TaqI TCGA 2 cut(s) 272, 562
TasI AATT 3 cut(s) 208, 461, 537
TatI WGTACW 1 cut(s) 126
TfiI GAWTC 3 cut(s) 233, 373, 404
Tru1I TTAA 4 cut(s) 63, 315, 498, 525
Tru9I TTAA 4 cut(s) 63, 315, 498, 525
TscAI CASTG 2 cut(s) 13, 246
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 543
TspRI CASTG 2 cut(s) 13, 246
ZrmI AGTACT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.