Rorug02G0180300

Plasma membrane ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000545
Physical Location & Seq
Forward (+)
16708897 .. 16711547
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0180300.1

Sequence Viewer

Length: 987 bp
ATGAAGAACGGCAACACCAAGCTGATTCTTCTCCACCCTTACATCCAAAAACAAGGCAGCTCCAACCGGCTATGGCTTCTCACCTTCATGTCCTTGTCCACCCTCGCCTTTCTCCTCACAATCATTTACACCAGCGGATTGTATGCATATTCGACAGCAACTACAACCACCACCACAACCGCCAATATAGCCGCCGCCACAATAACTGCCACCAACGCCCCAATGCCGACCACCGTGATCAACACGCTCCTCCACTACGCGTCGAAATCAAACGACACGTTCAAAATGTCATACTCCGAGATCAAACCCATCTCGGACGTACTAAGAAAATGTTCCTCCCCTTGTAACTTCCTGATCTTCGGCCTCACCCACGAGACCCTTCTCTGGAAAGCCCTAAACCACAACGGCCGGACCGTCTTCATCGACGAGAACCGCTACTACGCTGCCTACATGGAAGAAAAGCACCCAGAAATCGACGCCTACGACGTTCAGTACACCACCAAGACTAGAGAGATGACCGAGTTGATCAAAGTCGCCAAAGAACAGATCAGAAACGAGTGCCGGCCGGTTCAGAATCTCTTGTTCTCGGAATGCAAGCTTGGAATCAATGATCTTCCGAACCATGTCTACGAAGTCGACTGGGACGTAATCTTGATCGACGGGCCCAGAGGAGACTGGCCCGACTCGCCGGGCCGGATCTCTCCCATTTTCACCTCCGGCGTTCTGGCCAGGAGCAAAAAGGGCGGGAGCGGGAAGACACACGTGTTCGTTCATGACTTCAGGAGGGACGTGGAGAGGGTCTGTGGGGATGAGTTTCTGTGCAGAGAGAATTTGGTGGAGGAGAGTGAGACGCTGGGGCATTATGTGGTGGAGAGGATGGAGGAGACTAGCTTCCAGTTCTGCCGCAACAACAACCGTAAAGTGACGTCATCAACGTCGGCGGCGGCAAATAATTCTTCTTCTTCTTCTTCGTCCTTGTCTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00190 map00190
Pfam Domains
Protein Families
Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

328

Amino Acids

36.95

Weight (kDa)

6.76

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 100 - 288 1.7e-81 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000545)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g36571
pyrus_communis pycom02g01560 pycom08g10820 pycom09g05820 pycom13g21850 pycom14g16010
rosa_chinensis RchiOBHm_Chr2g0094861 RchiOBHm_Chr2g0124911 RchiOBHm_Chr2g0138581 RchiOBHm_Chr2g0160851 RchiOBHm_Chr4g0407411
rosa_laevigata RLG00000001652 RLG00000001872 RLG00000002037 RLG00000003076 RLG00000006419 RLG00000016771 RLG00000017383 RLG00000022851 RLG00000030563 RLG00000033869 RLG00000034176
rosa_multiflora Rmu_co8013424.1_g000001 Rmu_co8016716.1_g000001 Rmu_co8183472.1_g000001 Rmu_co8254047.1_g000001 Rmu_co8354891.1_g000001 Rmu_sc0000124.1_g000003 Rmu_sc0002132.1_g000027 Rmu_sc0003007.1_g000011 Rmu_sc0003221.1_g000096 Rmu_sc0003221.1_g000097 Rmu_sc0005119.1_g000004 Rmu_sc0008057.1_g000005 Rmu_sc0034532.1_g000001 Rmu_sc0036406.1_g000001 Rmu_ssc0000008.1_g000009
rosa_roxburghii Rroxscaffold_1G00030520 Rroxscaffold_2G00107360 Rroxscaffold_2G00153420 Rroxscaffold_5G00335750 Rroxscaffold_5G00351760 Rroxscaffold_5G00352080 Rroxscaffold_6G00419090
rosa_rugosa Rorug01G0341800 Rorug01G0341900 Rorug02G0180200 Rorug02G0180300 Rorug03G0186100 Rorug03G0186200 Rorug03G0284800 Rorug04G0104500 Rorug05G0246300 Rorug06G0108400 Rorug06G0471500 Rorug06G0510400
rosa_samantha Rh1AG159000 Rh1CG148200 Rh1CG189900 Rh2AG552800 Rh2BG473900 Rh2BG612100 Rh2BG612300 Rh2DG094500 Rh2DG482600 Rh3BG234000 Rh3CG320000 Rh4AG144200 Rh4BG127900 Rh4DG097300 Rh5AG128900 Rh5AG381200 Rh5BG363000 Rh5DG491900 Rh6AG048400 Rh6BG168100 Rh6CG005800 Rh6DG006000 Rh7AG255100 Rh7BG360600 Rh7CG271700 Rh7CG482300 Rh7DG451200
rosa_wichuraiana Rw4G004970

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 929
AccBSI CCGCTC 1 cut(s) 750
AccI GTMKAC 2 cut(s) 627, 636
AccII CGCG 1 cut(s) 260
AclWI GGATC 1 cut(s) 704
AcoI YGGCCR 3 cut(s) 406, 563, 726
AcsI RAATTY 1 cut(s) 829
AcuI CTGAAG 1 cut(s) 763
AcvI CACGTG 1 cut(s) 763
AcyI GRCGYC 2 cut(s) 477, 926
AfaI GTAC 2 cut(s) 321, 494
AfiI CCNNNNNNNGG 1 cut(s) 384
AflIII ACRYGT 4 cut(s) 258, 276, 760, 762
AgsI TTSAA 1 cut(s) 283
AjiI CACGTC 1 cut(s) 790
AjnI CCWGG 1 cut(s) 728
AjuI GAANNNNNNNTTGG 2 cut(s) 582, 614
AluBI AGCT 4 cut(s) 22, 60, 598, 891
AluI AGCT 4 cut(s) 22, 60, 598, 891
Alw26I GTCTC 4 cut(s) 368, 666, 842, 878
AlwI GGATC 1 cut(s) 704
AoxI GGCC 7 cut(s) 361, 406, 563, 662, 677, 691, 726
ApaI GGGCCC 1 cut(s) 666
ApeKI GCWGC 2 cut(s) 57, 443
ApoI RAATTY 1 cut(s) 829
Asp700I GAANNNNTTC 1 cut(s) 331
AspS9I GGNCC 5 cut(s) 411, 662, 663, 678, 691
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 3 cut(s) 73, 358, 703
AvaII GGWCC 1 cut(s) 411
BaeGI GKGCMC 1 cut(s) 666
BalI TGGCCA 1 cut(s) 728
BanII GRGCYC 1 cut(s) 666
BauI CACGAG 1 cut(s) 371
BbrPI CACGTG 1 cut(s) 763
BbsI GAAGAC 2 cut(s) 409, 761
BbvI GCAGC 2 cut(s) 69, 430
BccI CCATC 2 cut(s) 317, 871
BceAI ACGGC 2 cut(s) 25, 421
BciT130I CCWGG 1 cut(s) 730
BclI TGATCA 2 cut(s) 237, 525
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 4 cut(s) 368, 666, 842, 878
BfaI CTAG 2 cut(s) 507, 888
BisI GCNGC 7 cut(s) 58, 192, 195, 444, 904, 942, 945
BlsI GCNGC 7 cut(s) 59, 193, 196, 445, 905, 943, 946
Bme1390I CCNGG 2 cut(s) 690, 730
Bme18I GGWCC 1 cut(s) 411
BmgBI CACGTC 1 cut(s) 790
BmgT120I GGNCC 5 cut(s) 411, 662, 663, 678, 691
BmiI GGNNCC 1 cut(s) 664
BmrFI CCNGG 2 cut(s) 690, 730
BmrI ACTGGG 1 cut(s) 649
BmuI ACTGGG 1 cut(s) 649
BpiI GAAGAC 2 cut(s) 409, 761
BpuMI CCSGG 1 cut(s) 690
BsaAI YACGTR 1 cut(s) 763
BsaHI GRCGYC 2 cut(s) 477, 926
BsaI GGTCTC 1 cut(s) 368
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse118I RCCGGY 3 cut(s) 66, 561, 565
Bse1I ACTGG 3 cut(s) 644, 680, 895
BseBI CCWGG 1 cut(s) 730
BseGI GGATG 3 cut(s) 42, 814, 882
BseLI CCNNNNNNNGG 1 cut(s) 384
BseNI ACTGG 3 cut(s) 644, 680, 895
BseRI GAGGAG 5 cut(s) 104, 239, 684, 854, 896
BseSI GKGCMC 1 cut(s) 666
BseX3I CGGCCG 2 cut(s) 406, 563
BseXI GCAGC 2 cut(s) 69, 430
BseYI CCCAGC 1 cut(s) 853
BsgI GTGCAG 1 cut(s) 841
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 2 cut(s) 409, 566
BshFI GGCC 7 cut(s) 363, 408, 565, 664, 679, 693, 728
BsiEI CGRYCG 2 cut(s) 409, 566
BsiSI CCGG 7 cut(s) 67, 409, 562, 566, 689, 694, 717
BslFI GGGAC 2 cut(s) 656, 800
BslI CCNNNNNNNGG 1 cut(s) 384
BsmAI GTCTC 4 cut(s) 368, 666, 842, 878
BsmBI CGTCTC 1 cut(s) 842
BsmFI GGGAC 2 cut(s) 656, 800
BsmI GAATGC 1 cut(s) 596
BsnI GGCC 7 cut(s) 363, 408, 565, 664, 679, 693, 728
Bso31I GGTCTC 1 cut(s) 368
Bsp120I GGGCCC 1 cut(s) 662
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 8 cut(s) 237, 300, 354, 525, 546, 610, 654, 696
BspANI GGCC 7 cut(s) 363, 408, 565, 664, 679, 693, 728
BspFNI CGCG 1 cut(s) 260
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 1 cut(s) 664
BspPI GGATC 1 cut(s) 704
BspTNI GGTCTC 1 cut(s) 368
BsrBI CCGCTC 1 cut(s) 750
BsrFI RCCGGY 3 cut(s) 66, 561, 565
BsrI ACTGG 3 cut(s) 644, 680, 895
BssAI RCCGGY 3 cut(s) 66, 561, 565
BssMI GATC 8 cut(s) 237, 300, 354, 525, 546, 610, 654, 696
BssNI GRCGYC 2 cut(s) 477, 926
BssSI CACGAG 1 cut(s) 371
Bst2BI CACGAG 1 cut(s) 371
Bst2UI CCWGG 1 cut(s) 730
Bst4CI ACNGT 3 cut(s) 235, 415, 917
BstACI GRCGYC 2 cut(s) 477, 926
BstBAI YACGTR 1 cut(s) 763
BstC8I GCNNGC 2 cut(s) 563, 596
BstDEI CTNAG 2 cut(s) 323, 981
BstF5I GGATG 3 cut(s) 42, 814, 882
BstFNI CGCG 1 cut(s) 260
BstKTI GATC 8 cut(s) 240, 303, 357, 528, 549, 613, 657, 699
BstMAI GTCTC 4 cut(s) 368, 666, 842, 878
BstMBI GATC 8 cut(s) 237, 300, 354, 525, 546, 610, 654, 696
BstMCI CGRYCG 2 cut(s) 409, 566
BstMWI GCNNNNNNNGC 4 cut(s) 188, 215, 685, 741
BstNI CCWGG 1 cut(s) 730
BstSCI CCNGG 2 cut(s) 688, 728
BstSLI GKGCMC 1 cut(s) 666
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 2 cut(s) 69, 430
BstV2I GAAGAC 2 cut(s) 409, 761
BstX2I RGATCY 1 cut(s) 696
BstYI RGATCY 1 cut(s) 696
BstZI CGGCCG 2 cut(s) 406, 563
BsuRI GGCC 7 cut(s) 363, 408, 565, 664, 679, 693, 728
BtrI CACGTC 1 cut(s) 790
BtsCI GGATG 3 cut(s) 42, 814, 882
Cac8I GCNNGC 2 cut(s) 563, 596
CciI TCATGA 1 cut(s) 772
Cfr10I RCCGGY 3 cut(s) 66, 561, 565
Cfr13I GGNCC 5 cut(s) 411, 662, 663, 678, 691
CpoI CGGWCCG 1 cut(s) 411
CseI GACGC 3 cut(s) 249, 485, 859
Csp6I GTAC 2 cut(s) 320, 493
CspI CGGWCCG 1 cut(s) 411
CviAII CATG 4 cut(s) 88, 451, 623, 773
CviQI GTAC 2 cut(s) 320, 493
DdeI CTNAG 2 cut(s) 323, 981
DpnI GATC 8 cut(s) 239, 302, 356, 527, 548, 612, 656, 698
DpnII GATC 8 cut(s) 237, 300, 354, 525, 546, 610, 654, 696
EaeI YGGCCR 3 cut(s) 406, 563, 726
EagI CGGCCG 2 cut(s) 406, 563
EclXI CGGCCG 2 cut(s) 406, 563
Eco24I GRGCYC 1 cut(s) 666
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 411
Eco52I CGGCCG 2 cut(s) 406, 563
Eco57I CTGAAG 1 cut(s) 763
Eco72I CACGTG 1 cut(s) 763
EcoRII CCWGG 1 cut(s) 728
EcoT22I ATGCAT 1 cut(s) 148
EcoT38I GRGCYC 1 cut(s) 666
Esp3I CGTCTC 1 cut(s) 842
FaeI CATG 4 cut(s) 91, 454, 626, 776
FaqI GGGAC 2 cut(s) 656, 800
FatI CATG 4 cut(s) 87, 450, 622, 772
FauI CCCGC 2 cut(s) 737, 743
FbaI TGATCA 2 cut(s) 237, 525
FblI GTMKAC 2 cut(s) 627, 636
Fnu4HI GCNGC 7 cut(s) 58, 192, 195, 444, 904, 942, 945
FokI GGATG 3 cut(s) 29, 821, 889
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 7 cut(s) 58, 192, 195, 444, 904, 942, 945
FspBI CTAG 2 cut(s) 507, 888
GluI GCNGC 7 cut(s) 58, 192, 195, 444, 904, 942, 945
GsaI CCCAGC 1 cut(s) 857
HaeIII GGCC 7 cut(s) 363, 408, 565, 664, 679, 693, 728
HapII CCGG 7 cut(s) 67, 409, 562, 566, 689, 694, 717
HgaI GACGC 3 cut(s) 249, 485, 859
Hin1I GRCGYC 2 cut(s) 477, 926
Hin1II CATG 4 cut(s) 91, 454, 626, 776
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HindIII AAGCTT 1 cut(s) 596
HinfI GANTC 4 cut(s) 25, 574, 603, 683
HpaII CCGG 7 cut(s) 67, 409, 562, 566, 689, 694, 717
HphI GGTGA 3 cut(s) 73, 358, 703
Hpy166II GTNNAC 4 cut(s) 99, 495, 628, 637
Hpy188I TCNGA 6 cut(s) 298, 316, 551, 573, 589, 618
Hpy188III TCNNGA 5 cut(s) 352, 385, 652, 773, 781
Hpy8I GTNNAC 4 cut(s) 99, 495, 628, 637
Hpy99I CGWCG 6 cut(s) 265, 428, 479, 488, 662, 940
HpyAV CCTTC 2 cut(s) 94, 389
HpyCH4III ACNGT 3 cut(s) 235, 415, 917
HpyCH4IV ACGT 8 cut(s) 278, 318, 486, 645, 762, 789, 926, 935
HpyCH4V TGCA 3 cut(s) 146, 594, 822
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 215, 685, 741
HpyF3I CTNAG 2 cut(s) 323, 981
HpySE526I ACGT 8 cut(s) 278, 318, 486, 645, 762, 789, 926, 935
Hsp92I GRCGYC 2 cut(s) 477, 926
Hsp92II CATG 4 cut(s) 91, 454, 626, 776
KroI GCCGGC 1 cut(s) 561
KroNI GCCGGC 1 cut(s) 563
Ksp22I TGATCA 2 cut(s) 237, 525
Kzo9I GATC 8 cut(s) 237, 300, 354, 525, 546, 610, 654, 696
LmnI GCTCC 4 cut(s) 65, 252, 732, 747
Lsp1109I GCAGC 2 cut(s) 69, 430
MaeI CTAG 2 cut(s) 507, 888
MaeII ACGT 8 cut(s) 278, 318, 486, 645, 762, 789, 926, 935
MaeIII GTNAC 2 cut(s) 344, 922
MalI GATC 8 cut(s) 239, 302, 356, 527, 548, 612, 656, 698
MbiI CCGCTC 1 cut(s) 750
MboI GATC 8 cut(s) 237, 300, 354, 525, 546, 610, 654, 696
MflI RGATCY 1 cut(s) 696
MhlI GDGCHC 1 cut(s) 666
MlsI TGGCCA 1 cut(s) 728
MluCI AATT 2 cut(s) 829, 952
MluI ACGCGT 1 cut(s) 258
MluNI TGGCCA 1 cut(s) 728
MlyI GAGTC 1 cut(s) 677
MmeI TCCRAC 1 cut(s) 87
Mox20I TGGCCA 1 cut(s) 728
Mph1103I ATGCAT 1 cut(s) 148
MroNI GCCGGC 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 331
MscI TGGCCA 1 cut(s) 728
MslI CAYNNNNRTG 1 cut(s) 86
Msp20I TGGCCA 1 cut(s) 728
MspA1I CMGCKG 1 cut(s) 135
MspI CCGG 7 cut(s) 67, 409, 562, 566, 689, 694, 717
MspR9I CCNGG 2 cut(s) 690, 730
Mva1269I GAATGC 1 cut(s) 596
MvaI CCWGG 1 cut(s) 730
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 4 cut(s) 188, 215, 685, 741
NaeI GCCGGC 1 cut(s) 563
NciI CCSGG 1 cut(s) 690
NdeII GATC 8 cut(s) 237, 300, 354, 525, 546, 610, 654, 696
NgoMIV GCCGGC 1 cut(s) 561
NlaIII CATG 4 cut(s) 91, 454, 626, 776
NlaIV GGNNCC 1 cut(s) 664
NmuCI GTSAC 1 cut(s) 922
NsiI ATGCAT 1 cut(s) 148
PagI TCATGA 1 cut(s) 772
PcsI WCGNNNNNNNCGW 5 cut(s) 411, 480, 483, 642, 932
PctI GAATGC 1 cut(s) 596
PdiI GCCGGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 331
PfeI GAWTC 3 cut(s) 25, 574, 603
PkrI GCNGC 7 cut(s) 59, 193, 196, 445, 905, 943, 946
PleI GAGTC 1 cut(s) 677
PmaCI CACGTG 1 cut(s) 763
PmlI CACGTG 1 cut(s) 763
PpsI GAGTC 1 cut(s) 677
Ppu21I YACGTR 1 cut(s) 763
Psp6I CCWGG 1 cut(s) 728
PspCI CACGTG 1 cut(s) 763
PspFI CCCAGC 1 cut(s) 853
PspGI CCWGG 1 cut(s) 728
PspN4I GGNNCC 1 cut(s) 664
PspOMI GGGCCC 1 cut(s) 662
PspPI GGNCC 5 cut(s) 411, 662, 663, 678, 691
PsrI GAACNNNNNNTAC 4 cut(s) 422, 454, 611, 643
PsuI RGATCY 1 cut(s) 696
RsaI GTAC 2 cut(s) 321, 494
RsaNI GTAC 2 cut(s) 320, 493
RseI CAYNNNNRTG 1 cut(s) 86
Rsr2I CGGWCCG 1 cut(s) 411
RsrII CGGWCCG 1 cut(s) 411
SalI GTCGAC 1 cut(s) 635
SatI GCNGC 7 cut(s) 58, 192, 195, 444, 904, 942, 945
Sau3AI GATC 8 cut(s) 237, 300, 354, 525, 546, 610, 654, 696
Sau96I GGNCC 5 cut(s) 411, 662, 663, 678, 691
SchI GAGTC 1 cut(s) 677
ScrFI CCNGG 2 cut(s) 690, 730
SduI GDGCHC 1 cut(s) 666
SinI GGWCC 1 cut(s) 411
SmiMI CAYNNNNRTG 1 cut(s) 86
Sse9I AATT 2 cut(s) 829, 952
SspMI CTAG 2 cut(s) 507, 888
StyD4I CCNGG 2 cut(s) 688, 728
TaaI ACNGT 3 cut(s) 235, 415, 917
TaiI ACGT 8 cut(s) 281, 321, 489, 648, 765, 792, 929, 938
TaqI TCGA 6 cut(s) 152, 263, 423, 474, 636, 657
TaqII GACCGA 1 cut(s) 533
TasI AATT 2 cut(s) 829, 952
TatI WGTACW 1 cut(s) 492
TauI GCSGC 5 cut(s) 194, 197, 906, 944, 947
TfiI GAWTC 3 cut(s) 25, 574, 603
TseFI GTSAC 1 cut(s) 922
TseI GCWGC 2 cut(s) 57, 443
Tsp45I GTSAC 1 cut(s) 922
TspDTI ATGAA 4 cut(s) 17, 76, 409, 761
VpaK11BI GGWCC 1 cut(s) 411
XapI RAATTY 1 cut(s) 829
XmiI GTMKAC 2 cut(s) 627, 636
XmnI GAANNNNTTC 1 cut(s) 331
XspI CTAG 2 cut(s) 507, 888
ZraI GACGTC 1 cut(s) 927
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.