pycom15g20370

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000386
Physical Location & Seq
Forward (+)
14553757 .. 14554729
973 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 891 bp
ATGGCAAAAGAGCATGTTAGAGGTCGTAATTGGACCTTTGACGAAGATATTGCTTTATGTTTGGCATGGATTTCGATTAGTGAAGATGGTGCCGTCGGCACCAATCAAAATAGAAAGGTTTTATGGGGTAAAATCGTTGATAAGTTCCATGAAAACTCCAACGCCGGTCGAAGGGAAGTTGGTGGTGTTTATGATCGGTGGAAGATTATCAACAAAGCGTGCACTTTGTGGAAAGGAAGCTTGGAGAGAGCCATGGTTGACATGCCTAGTGGAAGGGGCGCCTCAGAAATTGGTGACAAAGCAATGGCAATTTACAAGACAAGAACTACACCAAAAAATCAAGCTTTTAAGTTGCATCATGCTTGGAACATCCTCAAGGATTGTCCGAGGTGGGGAACCGATGCGAACCAACAATGTGGAAGATTATTTCATAATGAAGCCCCACCCCCAAATGATGTCAATGAAGGTGTGAATTTTGCCGACAATGAAGGTGTCGAACAAATGACCCCAACTTCTTCTTTTGCAAGGCCCCCGGGTAGAGATAAGCAAAAGGAAGCAAAGAGAAAAGGGAAGTCCCAAGATCCGACACATGCACAATTTGCTAGCGAAATGGCAATAATGAAGGAAATCCAGTGCCGTCGGCAAGAAGAATCGGCCCAAATGCTTTTGGCCATGAAGGAACAAGGGGATAGGGAGCAAGAAAGGTACGAAACTAATTTGATAATGGAGGACCTCGACAAATACACTCCAGAGAGGAAGAGATACTTACGTGGTAAGCAAAAGGAAATTTTACGAAGGAATGCCACAAGGAGTATATTTCAAGATGATGATTCATCTCAAGACTATCACCCAAGTCCATCACCAAGTCAAGATGGTGAATATCATTATTAG

Protein Analysis

297

Amino Acids

34.03

Weight (kDa)

8.18

Isoelectric Point (pI)

56.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 115 - 263 3.1e-13 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000386)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g44301 FvH4_5g19180 FvH4_6g21610 FvH4_6g22213 FvH4_6g33100 FvH4_6g34882
malus_domestica MD00G1014200.v1.1 MD04G1097600.v1.1 MD13G1034300.v1.1
pyrus_communis pycom01g05740 pycom04g10190 pycom05g05910 pycom05g14840 pycom07g10040 pycom08g16560 pycom09g00370 pycom10g03940 pycom10g15460 pycom11g15220 pycom12g07590 pycom14g10750 pycom14g14610 pycom15g20370
rosa_chinensis RchiOBHm_Chr4g0392971 RchiOBHm_Chr4g0409971 RchiOBHm_Chr5g0059411 RchiOBHm_Chr6g0305091 RchiOBHm_Chr7g0202551 RchiOBHm_Chr7g0242211
rosa_laevigata RLG00000003630 RLG00000016594
rosa_multiflora Rmu_sc0000018.1_g000003 Rmu_sc0000288.1_g000031 Rmu_sc0000327.1_g000003 Rmu_sc0000361.1_g000009 Rmu_sc0000546.1_g000077 Rmu_sc0000616.1_g000024 Rmu_sc0000623.1_g000005 Rmu_sc0000704.1_g000009 Rmu_sc0000749.1_g000026 Rmu_sc0000965.1_g000012 Rmu_sc0001144.1_g000024 Rmu_sc0001144.1_g000026 Rmu_sc0001159.1_g000053 Rmu_sc0001348.1_g000017 Rmu_sc0001585.1_g000015 Rmu_sc0001651.1_g000017 Rmu_sc0001832.1_g000013 Rmu_sc0001838.1_g000003 Rmu_sc0001977.1_g000020 Rmu_sc0002983.1_g000011 Rmu_sc0003270.1_g000026 Rmu_sc0003317.1_g000014 Rmu_sc0003517.1_g000013 Rmu_sc0003543.1_g000011 Rmu_sc0003629.1_g000008 Rmu_sc0003731.1_g000005 Rmu_sc0004088.1_g000005 Rmu_sc0004088.1_g000006 Rmu_sc0004094.1_g000035 Rmu_sc0004324.1_g000006 Rmu_sc0005106.1_g000004 Rmu_sc0005198.1_g000006 Rmu_sc0005599.1_g000009 Rmu_sc0005762.1_g000008 Rmu_sc0006168.1_g000029 Rmu_sc0006273.1_g000014 Rmu_sc0006399.1_g000010 Rmu_sc0006632.1_g000010 Rmu_sc0006656.1_g000001 Rmu_sc0006875.1_g000002 Rmu_sc0007017.1_g000006 Rmu_sc0007034.1_g000017 Rmu_sc0007840.1_g000007 Rmu_sc0009945.1_g000003 Rmu_sc0010071.1_g000002 Rmu_sc0010503.1_g000005 Rmu_sc0011218.1_g000008 Rmu_sc0014846.1_g000007 Rmu_sc0015852.1_g000002 Rmu_sc0029270.1_g000003 Rmu_sc0036358.1_g000001 Rmu_ssc0000050.1_g000071 Rmu_ssc0000110.1_g000008 Rmu_ssc0000267.1_g000009
rosa_roxburghii Rroxscaffold_2G00100340 Rroxscaffold_2G00144280 Rroxscaffold_3G00221910
rosa_rugosa Rorug04G0175800
rosa_wichuraiana Rw0G012500 Rw0G013760 Rw0G014530 Rw0G021600 Rw2G019730 Rw2G045390 Rw3G001890 Rw3G025260 Rw3G029050 Rw4G000250 Rw5G014160 Rw5G031520 Rw5G036440 Rw6G023350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 89, 98, 278
AclWI GGATC 1 cut(s) 575
AcoI YGGCCR 1 cut(s) 669
AcsI RAATTY 2 cut(s) 472, 786
AcyI GRCGYC 1 cut(s) 279
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 1 cut(s) 707
AfiI CCNNNNNNNGG 2 cut(s) 171, 392
AgsI TTSAA 1 cut(s) 821
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 2 cut(s) 240, 344
AluI AGCT 2 cut(s) 240, 344
Alw21I GWGCWC 1 cut(s) 224
Alw44I GTGCAC 1 cut(s) 220
AlwI GGATC 1 cut(s) 575
Ama87I CYCGRG 1 cut(s) 532
AoxI GGCC 3 cut(s) 527, 654, 669
ApaLI GTGCAC 1 cut(s) 220
ApoI RAATTY 2 cut(s) 472, 786
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 4 cut(s) 33, 528, 655, 730
AsuC2I CCSGG 2 cut(s) 533, 534
AsuHPI GGTGA 4 cut(s) 305, 839, 852, 887
AsuNHI GCTAGC 1 cut(s) 602
AvaI CYCGRG 1 cut(s) 532
AvaII GGWCC 2 cut(s) 33, 730
BaeGI GKGCMC 1 cut(s) 224
BaeI ACNNNNGTAYC 2 cut(s) 754, 787
BalI TGGCCA 1 cut(s) 671
BanI GGYRCC 3 cut(s) 89, 98, 278
Bbv12I GWGCWC 1 cut(s) 224
BccI CCATC 3 cut(s) 80, 865, 866
BceAI ACGGC 2 cut(s) 77, 621
BcgI CGANNNNNNTGC 4 cut(s) 32, 54, 66, 88
BcnI CCSGG 2 cut(s) 533, 534
BfaI CTAG 2 cut(s) 267, 603
BfoI RGCGCY 1 cut(s) 282
Bme1390I CCNGG 2 cut(s) 533, 534
Bme18I GGWCC 2 cut(s) 33, 730
BmeT110I CYCGRG 1 cut(s) 532
BmgT120I GGNCC 4 cut(s) 33, 528, 655, 730
BmiI GGNNCC 5 cut(s) 91, 100, 280, 397, 530
BmrFI CCNGG 2 cut(s) 533, 534
BmsI GCATC 2 cut(s) 364, 391
BmtI GCTAGC 1 cut(s) 606
BpmI CTGGAG 1 cut(s) 732
BpuEI CTTGAG 2 cut(s) 359, 822
BpuMI CCSGG 2 cut(s) 533, 534
BsaAI YACGTR 1 cut(s) 770
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 4 cut(s) 252, 386, 531, 532
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 392
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 1 cut(s) 631
Bse3DI GCAATG 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 252, 386, 531, 532
BseGI GGATG 1 cut(s) 369
BseLI CCNNNNNNNGG 2 cut(s) 171, 392
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 631
BseSI GKGCMC 1 cut(s) 224
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 3 cut(s) 529, 656, 671
BshNI GGYRCC 3 cut(s) 89, 98, 278
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 224
BsiHKCI CYCGRG 1 cut(s) 532
BsiSI CCGG 2 cut(s) 165, 533
BslFI GGGAC 1 cut(s) 559
BslI CCNNNNNNNGG 2 cut(s) 171, 392
BsmFI GGGAC 1 cut(s) 559
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 3 cut(s) 529, 656, 671
BsoBI CYCGRG 1 cut(s) 532
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 2 cut(s) 193, 580
Bsp19I CCATGG 1 cut(s) 252
BspANI GGCC 3 cut(s) 529, 656, 671
BspCNI CTCAG 1 cut(s) 296
BspLI GGNNCC 5 cut(s) 91, 100, 280, 397, 530
BspOI GCTAGC 1 cut(s) 606
BspPI GGATC 1 cut(s) 575
BspT107I GGYRCC 3 cut(s) 89, 98, 278
BsrDI GCAATG 1 cut(s) 309
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 1 cut(s) 631
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 252, 386, 531, 532
BssMI GATC 2 cut(s) 193, 580
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 252
Bst6I CTCTTC 1 cut(s) 752
BstACI GRCGYC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 599
BstBAI YACGTR 1 cut(s) 770
BstC8I GCNNGC 2 cut(s) 220, 604
BstDEI CTNAG 1 cut(s) 283
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 1 cut(s) 369
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 2 cut(s) 196, 583
BstMBI GATC 2 cut(s) 193, 580
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNSI RCATGY 3 cut(s) 17, 265, 593
BstSCI CCNGG 2 cut(s) 531, 532
BstSLI GKGCMC 1 cut(s) 224
BstX2I RGATCY 1 cut(s) 580
BstXI CCANNNNNNTGG 1 cut(s) 416
BstYI RGATCY 1 cut(s) 580
BsuRI GGCC 3 cut(s) 529, 656, 671
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 638
Cac8I GCNNGC 2 cut(s) 220, 604
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 4 cut(s) 33, 528, 655, 730
Cfr9I CCCGGG 1 cut(s) 532
Csp6I GTAC 1 cut(s) 706
CviAII CATG 8 cut(s) 14, 66, 149, 253, 262, 359, 590, 673
CviJI RGCY 7 cut(s) 240, 251, 344, 440, 529, 656, 671
CviKI_1 RGCY 7 cut(s) 240, 251, 344, 440, 529, 656, 671
CviQI GTAC 1 cut(s) 706
DdeI CTNAG 1 cut(s) 283
DinI GGCGCC 1 cut(s) 280
DpnI GATC 2 cut(s) 195, 582
DpnII GATC 2 cut(s) 193, 580
DraIII CACNNNGTG 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 669
Eam1104I CTCTTC 1 cut(s) 752
EarI CTCTTC 1 cut(s) 752
Eco130I CCWWGG 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 33, 730
Eco88I CYCGRG 1 cut(s) 532
EcoO109I RGGNCCY 2 cut(s) 528, 730
EcoT14I CCWWGG 1 cut(s) 252
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 8 cut(s) 17, 69, 152, 256, 265, 362, 593, 676
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FaqI GGGAC 1 cut(s) 559
FatI CATG 8 cut(s) 13, 65, 148, 252, 261, 358, 589, 672
FokI GGATG 1 cut(s) 356
FspBI CTAG 2 cut(s) 267, 603
GlaI GCGC 1 cut(s) 280
GsuI CTGGAG 1 cut(s) 732
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 3 cut(s) 529, 656, 671
HapII CCGG 2 cut(s) 165, 533
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 8 cut(s) 17, 69, 152, 256, 265, 362, 593, 676
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HindIII AAGCTT 2 cut(s) 238, 342
HinfI GANTC 2 cut(s) 650, 830
HpaII CCGG 2 cut(s) 165, 533
HphI GGTGA 4 cut(s) 305, 839, 852, 887
Hpy166II GTNNAC 2 cut(s) 222, 259
Hpy188I TCNGA 3 cut(s) 286, 387, 585
Hpy188III TCNNGA 4 cut(s) 749, 821, 839, 869
Hpy8I GTNNAC 2 cut(s) 222, 259
Hpy99I CGWCG 2 cut(s) 98, 642
HpyAV CCTTC 7 cut(s) 165, 267, 458, 482, 616, 670, 789
HpyCH4IV ACGT 1 cut(s) 769
HpyCH4V TGCA 4 cut(s) 222, 355, 524, 593
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpyF3I CTNAG 1 cut(s) 283
HpySE526I ACGT 1 cut(s) 769
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 8 cut(s) 17, 69, 152, 256, 265, 362, 593, 676
HspAI GCGC 1 cut(s) 279
KasI GGCGCC 1 cut(s) 278
Kzo9I GATC 2 cut(s) 193, 580
LmnI GCTCC 1 cut(s) 694
LpnPI CCDG 4 cut(s) 178, 546, 644, 762
LweI GCATC 2 cut(s) 364, 391
MaeI CTAG 2 cut(s) 267, 603
MaeII ACGT 1 cut(s) 769
MaeIII GTNAC 1 cut(s) 293
MalI GATC 2 cut(s) 195, 582
MboI GATC 2 cut(s) 193, 580
MboII GAAGA 7 cut(s) 56, 95, 214, 432, 507, 659, 769
MflI RGATCY 1 cut(s) 580
MhlI GDGCHC 1 cut(s) 224
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 7 cut(s) 28, 288, 309, 472, 596, 715, 786
MluNI TGGCCA 1 cut(s) 671
Mly113I GGCGCC 1 cut(s) 279
MmeI TCCRAC 2 cut(s) 183, 608
MnlI CCTC 7 cut(s) 14, 292, 381, 383, 721, 743, 747
Mox20I TGGCCA 1 cut(s) 671
MscI TGGCCA 1 cut(s) 671
MseI TTAA 1 cut(s) 348
Msp20I TGGCCA 1 cut(s) 671
MspI CCGG 2 cut(s) 165, 533
MspR9I CCNGG 2 cut(s) 533, 534
Mva1269I GAATGC 1 cut(s) 805
MwoI GCNNNNNNNGC 1 cut(s) 599
NarI GGCGCC 1 cut(s) 279
NciI CCSGG 2 cut(s) 533, 534
NcoI CCATGG 1 cut(s) 252
NdeII GATC 2 cut(s) 193, 580
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 8 cut(s) 17, 69, 152, 256, 265, 362, 593, 676
NlaIV GGNNCC 5 cut(s) 91, 100, 280, 397, 530
NmuCI GTSAC 1 cut(s) 293
NspI RCATGY 3 cut(s) 17, 265, 593
PctI GAATGC 1 cut(s) 805
PfeI GAWTC 2 cut(s) 650, 830
PluTI GGCGCC 1 cut(s) 282
Ppu21I YACGTR 1 cut(s) 770
PpuMI RGGWCCY 1 cut(s) 730
Psp5II RGGWCCY 1 cut(s) 730
PspN4I GGNNCC 5 cut(s) 91, 100, 280, 397, 530
PspPI GGNCC 4 cut(s) 33, 528, 655, 730
PspPPI RGGWCCY 1 cut(s) 730
PsuI RGATCY 1 cut(s) 580
RsaI GTAC 1 cut(s) 707
RsaNI GTAC 1 cut(s) 706
SaqAI TTAA 1 cut(s) 348
Sau3AI GATC 2 cut(s) 193, 580
Sau96I GGNCC 4 cut(s) 33, 528, 655, 730
ScrFI CCNGG 2 cut(s) 533, 534
SduI GDGCHC 1 cut(s) 224
SfaNI GCATC 2 cut(s) 364, 391
SfoI GGCGCC 1 cut(s) 280
SinI GGWCC 2 cut(s) 33, 730
SmaI CCCGGG 1 cut(s) 534
SmlI CTYRAG 2 cut(s) 374, 837
SmoI CTYRAG 2 cut(s) 374, 837
Sse9I AATT 7 cut(s) 28, 288, 309, 472, 596, 715, 786
SspDI GGCGCC 1 cut(s) 278
SspMI CTAG 2 cut(s) 267, 603
StyD4I CCNGG 2 cut(s) 531, 532
StyI CCWWGG 1 cut(s) 252
TaiI ACGT 1 cut(s) 772
TaqI TCGA 4 cut(s) 74, 169, 495, 735
TasI AATT 7 cut(s) 28, 288, 309, 472, 596, 715, 786
TfiI GAWTC 2 cut(s) 650, 830
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 1 cut(s) 638
TseFI GTSAC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 8 cut(s) 165, 419, 450, 477, 501, 635, 689, 822
TspMI CCCGGG 1 cut(s) 532
TspRI CASTG 1 cut(s) 638
VneI GTGCAC 1 cut(s) 220
VpaK11BI GGWCC 2 cut(s) 33, 730
XapI RAATTY 2 cut(s) 472, 786
XceI RCATGY 3 cut(s) 17, 265, 593
XmaI CCCGGG 1 cut(s) 532
XspI CTAG 2 cut(s) 267, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.