Heat shock protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1907 |
| Acc16I | TGCGCA | 1 cut(s) | 827 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 888 |
| AccI | GTMKAC | 1 cut(s) | 1713 |
| AccII | CGCG | 1 cut(s) | 114 |
| AccIII | TCCGGA | 2 cut(s) | 220, 1066 |
| AciI | CCGC | 4 cut(s) | 5, 258, 413, 2054 |
| AclWI | GGATC | 4 cut(s) | 269, 567, 596, 1650 |
| AcsI | RAATTY | 3 cut(s) | 617, 938, 1995 |
| AcuI | CTGAAG | 4 cut(s) | 1124, 1598, 1905, 2030 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 556, 888, 1853 |
| AflIII | ACRYGT | 1 cut(s) | 1815 |
| AgsI | TTSAA | 7 cut(s) | 938, 943, 1159, 1204, 1267, 1363, 1531 |
| AjnI | CCWGG | 2 cut(s) | 42, 992 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1253, 1285 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1467, 1499 |
| Alw21I | GWGCWC | 2 cut(s) | 120, 1062 |
| Alw26I | GTCTC | 5 cut(s) | 23, 474, 1668, 1931, 2033 |
| AlwI | GGATC | 4 cut(s) | 269, 567, 596, 1650 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 64 |
| Ama87I | CYCGRG | 2 cut(s) | 1128, 1990 |
| Aor13HI | TCCGGA | 2 cut(s) | 220, 1066 |
| AoxI | GGCC | 2 cut(s) | 45, 1390 |
| ApeKI | GCWGC | 6 cut(s) | 517, 823, 1725, 1924, 1927, 2078 |
| ApoI | RAATTY | 3 cut(s) | 617, 938, 1995 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1930, 1962 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 914 |
| AspLEI | GCGC | 1 cut(s) | 828 |
| AspS9I | GGNCC | 2 cut(s) | 1390, 1915 |
| AsuC2I | CCSGG | 1 cut(s) | 1679 |
| AsuHPI | GGTGA | 8 cut(s) | 298, 524, 835, 1174, 1355, 1430, 1710, 1751 |
| AvaI | CYCGRG | 2 cut(s) | 1128, 1990 |
| AvaII | GGWCC | 1 cut(s) | 1915 |
| AxyI | CCTNAGG | 1 cut(s) | 834 |
| BaeGI | GKGCMC | 1 cut(s) | 302 |
| BanII | GRGCYC | 3 cut(s) | 120, 973, 1062 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1388, 1420 |
| BbsI | GAAGAC | 2 cut(s) | 659, 1835 |
| Bbv12I | GWGCWC | 2 cut(s) | 120, 1062 |
| BbvCI | CCTCAGC | 2 cut(s) | 1871, 2082 |
| BbvI | GCAGC | 6 cut(s) | 529, 810, 1712, 1911, 1914, 2090 |
| BccI | CCATC | 4 cut(s) | 10, 641, 956, 1535 |
| BciT130I | CCWGG | 2 cut(s) | 44, 994 |
| BclI | TGATCA | 2 cut(s) | 72, 1164 |
| BcnI | CCSGG | 1 cut(s) | 1679 |
| BcoDI | GTCTC | 5 cut(s) | 23, 474, 1668, 1931, 2033 |
| BfmI | CTRYAG | 1 cut(s) | 88 |
| BisI | GCNGC | 6 cut(s) | 518, 824, 1726, 1925, 1928, 2079 |
| BlpI | GCTNAGC | 2 cut(s) | 192, 2021 |
| BlsI | GCNGC | 6 cut(s) | 519, 825, 1727, 1926, 1929, 2080 |
| Bme1390I | CCNGG | 3 cut(s) | 44, 994, 1679 |
| Bme18I | GGWCC | 1 cut(s) | 1915 |
| BmeT110I | CYCGRG | 2 cut(s) | 1128, 1990 |
| BmgT120I | GGNCC | 2 cut(s) | 1390, 1915 |
| BmiI | GGNNCC | 2 cut(s) | 319, 972 |
| BmrFI | CCNGG | 3 cut(s) | 44, 994, 1679 |
| BpiI | GAAGAC | 2 cut(s) | 659, 1835 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1238, 1270 |
| BpmI | CTGGAG | 2 cut(s) | 369, 1771 |
| Bpu10I | CCTNAGC | 3 cut(s) | 1782, 1871, 2082 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 192, 2021 |
| BpuEI | CTTGAG | 2 cut(s) | 536, 1448 |
| BpuMI | CCSGG | 1 cut(s) | 1679 |
| BsaI | GGTCTC | 1 cut(s) | 1668 |
| BsaJI | CCNNGG | 3 cut(s) | 321, 846, 1833 |
| BsaWI | WCCGGW | 2 cut(s) | 220, 1066 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 548, 578, 1665, 1695 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 556, 888, 1853 |
| Bse1I | ACTGG | 2 cut(s) | 1420, 1741 |
| Bse21I | CCTNAGG | 1 cut(s) | 834 |
| BseAI | TCCGGA | 2 cut(s) | 220, 1066 |
| BseBI | CCWGG | 2 cut(s) | 44, 994 |
| BseDI | CCNNGG | 3 cut(s) | 321, 846, 1833 |
| BseGI | GGATG | 9 cut(s) | 13, 508, 694, 856, 948, 1504, 2010, 2056, 2080 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 556, 888, 1853 |
| BseNI | ACTGG | 2 cut(s) | 1420, 1741 |
| BseRI | GAGGAG | 6 cut(s) | 598, 1042, 1070, 1634, 1880, 2099 |
| BseSI | GKGCMC | 1 cut(s) | 302 |
| BseXI | GCAGC | 6 cut(s) | 529, 810, 1712, 1911, 1914, 2090 |
| BseYI | CCCAGC | 1 cut(s) | 2014 |
| Bsh1236I | CGCG | 1 cut(s) | 114 |
| BshFI | GGCC | 2 cut(s) | 47, 1392 |
| BsiHKAI | GWGCWC | 2 cut(s) | 120, 1062 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1128, 1990 |
| BsiSI | CCGG | 4 cut(s) | 197, 221, 1067, 1678 |
| BslFI | GGGAC | 2 cut(s) | 1536, 1808 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 556, 888, 1853 |
| BsmAI | GTCTC | 5 cut(s) | 23, 474, 1668, 1931, 2033 |
| BsmBI | CGTCTC | 1 cut(s) | 23 |
| BsmFI | GGGAC | 2 cut(s) | 1536, 1808 |
| BsmI | GAATGC | 3 cut(s) | 38, 610, 1250 |
| BsnI | GGCC | 2 cut(s) | 47, 1392 |
| Bso31I | GGTCTC | 1 cut(s) | 1668 |
| BsoBI | CYCGRG | 2 cut(s) | 1128, 1990 |
| Bsp1286I | GDGCHC | 4 cut(s) | 120, 302, 973, 1062 |
| Bsp13I | TCCGGA | 2 cut(s) | 220, 1066 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 192, 2021 |
| Bsp19I | CCATGG | 1 cut(s) | 1833 |
| Bsp68I | TCGCGA | 1 cut(s) | 114 |
| BspACI | CCGC | 4 cut(s) | 5, 258, 413, 2054 |
| BspANI | GGCC | 2 cut(s) | 47, 1392 |
| BspEI | TCCGGA | 2 cut(s) | 220, 1066 |
| BspFNI | CGCG | 1 cut(s) | 114 |
| BspHI | TCATGA | 2 cut(s) | 784, 1279 |
| BspLI | GGNNCC | 2 cut(s) | 319, 972 |
| BspPI | GGATC | 4 cut(s) | 269, 567, 596, 1650 |
| BspTNI | GGTCTC | 1 cut(s) | 1668 |
| BsrI | ACTGG | 2 cut(s) | 1420, 1741 |
| BssECI | CCNNGG | 3 cut(s) | 321, 846, 1833 |
| BssT1I | CCWWGG | 3 cut(s) | 321, 846, 1833 |
| Bst2UI | CCWGG | 2 cut(s) | 44, 994 |
| Bst4CI | ACNGT | 4 cut(s) | 378, 493, 1052, 2044 |
| Bst6I | CTCTTC | 5 cut(s) | 551, 573, 678, 1596, 1871 |
| BstC8I | GCNNGC | 3 cut(s) | 116, 182, 830 |
| BstDSI | CCRYGG | 1 cut(s) | 1833 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 554 |
| BstF5I | GGATG | 9 cut(s) | 13, 508, 694, 856, 948, 1504, 2010, 2056, 2080 |
| BstFNI | CGCG | 1 cut(s) | 114 |
| BstHHI | GCGC | 1 cut(s) | 828 |
| BstMAI | GTCTC | 5 cut(s) | 23, 474, 1668, 1931, 2033 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 44, 264, 829 |
| BstNI | CCWGG | 2 cut(s) | 44, 994 |
| BstNSI | RCATGY | 2 cut(s) | 1819, 2062 |
| BstSCI | CCNGG | 3 cut(s) | 42, 992, 1677 |
| BstSFI | CTRYAG | 1 cut(s) | 88 |
| BstSLI | GKGCMC | 1 cut(s) | 302 |
| BstUI | CGCG | 1 cut(s) | 114 |
| BstV1I | GCAGC | 6 cut(s) | 529, 810, 1712, 1911, 1914, 2090 |
| BstV2I | GAAGAC | 2 cut(s) | 659, 1835 |
| BstX2I | RGATCY | 1 cut(s) | 1642 |
| BstYI | RGATCY | 1 cut(s) | 1642 |
| Bsu36I | CCTNAGG | 1 cut(s) | 834 |
| BsuRI | GGCC | 2 cut(s) | 47, 1392 |
| BtgI | CCRYGG | 1 cut(s) | 1833 |
| BtsCI | GGATG | 9 cut(s) | 13, 508, 694, 856, 948, 1504, 2010, 2056, 2080 |
| BtsI | GCAGTG | 1 cut(s) | 1440 |
| BtsIMutI | CAGTG | 4 cut(s) | 489, 679, 1440, 1734 |
| BtuMI | TCGCGA | 1 cut(s) | 114 |
| Cac8I | GCNNGC | 3 cut(s) | 116, 182, 830 |
| CaiI | CAGNNNCTG | 1 cut(s) | 64 |
| CciI | TCATGA | 2 cut(s) | 784, 1279 |
| CfoI | GCGC | 1 cut(s) | 828 |
| Cfr13I | GGNCC | 2 cut(s) | 1390, 1915 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1907 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1907 |
| Eam1104I | CTCTTC | 5 cut(s) | 551, 573, 678, 1596, 1871 |
| EarI | CTCTTC | 5 cut(s) | 551, 573, 678, 1596, 1871 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Ecl136II | GAGCTC | 2 cut(s) | 118, 1060 |
| Eco130I | CCWWGG | 3 cut(s) | 321, 846, 1833 |
| Eco24I | GRGCYC | 3 cut(s) | 120, 973, 1062 |
| Eco31I | GGTCTC | 1 cut(s) | 1668 |
| Eco47I | GGWCC | 1 cut(s) | 1915 |
| Eco53kI | GAGCTC | 2 cut(s) | 118, 1060 |
| Eco57I | CTGAAG | 4 cut(s) | 1124, 1598, 1905, 2030 |
| Eco81I | CCTNAGG | 1 cut(s) | 834 |
| Eco88I | CYCGRG | 2 cut(s) | 1128, 1990 |
| EcoICRI | GAGCTC | 2 cut(s) | 118, 1060 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 554 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1915 |
| EcoRI | GAATTC | 3 cut(s) | 617, 938, 1995 |
| EcoRII | CCWGG | 2 cut(s) | 42, 992 |
| EcoT14I | CCWWGG | 3 cut(s) | 321, 846, 1833 |
| EcoT38I | GRGCYC | 3 cut(s) | 120, 973, 1062 |
| ErhI | CCWWGG | 3 cut(s) | 321, 846, 1833 |
| Esp3I | CGTCTC | 1 cut(s) | 23 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 918, 950 |
| FaqI | GGGAC | 2 cut(s) | 1536, 1808 |
| FauI | CCCGC | 1 cut(s) | 251 |
| FbaI | TGATCA | 2 cut(s) | 72, 1164 |
| FblI | GTMKAC | 1 cut(s) | 1713 |
| Fnu4HI | GCNGC | 6 cut(s) | 518, 824, 1726, 1925, 1928, 2079 |
| FokI | GGATG | 9 cut(s) | 20, 515, 701, 863, 935, 1511, 2017, 2063, 2087 |
| FriOI | GRGCYC | 3 cut(s) | 120, 973, 1062 |
| Fsp4HI | GCNGC | 6 cut(s) | 518, 824, 1726, 1925, 1928, 2079 |
| FspI | TGCGCA | 1 cut(s) | 827 |
| GlaI | GCGC | 1 cut(s) | 827 |
| GluI | GCNGC | 6 cut(s) | 518, 824, 1726, 1925, 1928, 2079 |
| GsaI | CCCAGC | 1 cut(s) | 2018 |
| GsuI | CTGGAG | 2 cut(s) | 369, 1771 |
| HaeIII | GGCC | 2 cut(s) | 47, 1392 |
| HapII | CCGG | 4 cut(s) | 197, 221, 1067, 1678 |
| HhaI | GCGC | 1 cut(s) | 828 |
| Hin6I | GCGC | 1 cut(s) | 826 |
| HinP1I | GCGC | 1 cut(s) | 826 |
| HincII | GTYRAC | 1 cut(s) | 1714 |
| HindII | GTYRAC | 1 cut(s) | 1714 |
| HindIII | AAGCTT | 4 cut(s) | 182, 336, 1357, 1550 |
| HinfI | GANTC | 9 cut(s) | 156, 163, 390, 467, 1100, 1276, 1592, 1715, 1959 |
| HpaII | CCGG | 4 cut(s) | 197, 221, 1067, 1678 |
| HphI | GGTGA | 8 cut(s) | 298, 524, 835, 1174, 1355, 1430, 1710, 1751 |
| Hpy166II | GTNNAC | 5 cut(s) | 289, 645, 1099, 1714, 2107 |
| Hpy188I | TCNGA | 9 cut(s) | 66, 136, 358, 616, 1105, 1322, 1578, 1597, 1702 |
| Hpy8I | GTNNAC | 5 cut(s) | 289, 645, 1099, 1714, 2107 |
| HpyCH4III | ACNGT | 4 cut(s) | 378, 493, 1052, 2044 |
| HpyCH4IV | ACGT | 3 cut(s) | 32, 83, 459 |
| HpyCH4V | TGCA | 7 cut(s) | 38, 308, 1191, 1502, 1650, 1757, 2078 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 44, 264, 829 |
| HpySE526I | ACGT | 3 cut(s) | 32, 83, 459 |
| HspAI | GCGC | 1 cut(s) | 826 |
| Kpn2I | TCCGGA | 2 cut(s) | 220, 1066 |
| Ksp22I | TGATCA | 2 cut(s) | 72, 1164 |
| LmnI | GCTCC | 4 cut(s) | 199, 976, 1057, 1867 |
| Lsp1109I | GCAGC | 6 cut(s) | 529, 810, 1712, 1911, 1914, 2090 |
| MaeII | ACGT | 3 cut(s) | 32, 83, 459 |
| MaeIII | GTNAC | 5 cut(s) | 427, 493, 1343, 1372, 1732 |
| MfeI | CAATTG | 3 cut(s) | 303, 1503, 1526 |
| MflI | RGATCY | 1 cut(s) | 1642 |
| MhlI | GDGCHC | 4 cut(s) | 120, 302, 973, 1062 |
| MluCI | AATT | 8 cut(s) | 127, 303, 617, 938, 1503, 1526, 1707, 1995 |
| MlyI | GAGTC | 4 cut(s) | 172, 476, 1094, 1709 |
| MmeI | TCCRAC | 3 cut(s) | 367, 546, 2049 |
| MroI | TCCGGA | 2 cut(s) | 220, 1066 |
| MroXI | GAANNNNTTC | 1 cut(s) | 914 |
| MseI | TTAA | 4 cut(s) | 12, 507, 1014, 1086 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1196 |
| MspA1I | CMGCKG | 1 cut(s) | 2081 |
| MspI | CCGG | 4 cut(s) | 197, 221, 1067, 1678 |
| MspR9I | CCNGG | 3 cut(s) | 44, 994, 1679 |
| MunI | CAATTG | 3 cut(s) | 303, 1503, 1526 |
| Mva1269I | GAATGC | 3 cut(s) | 38, 610, 1250 |
| MvaI | CCWGG | 2 cut(s) | 44, 994 |
| MvnI | CGCG | 1 cut(s) | 114 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 44, 264, 829 |
| NciI | CCSGG | 1 cut(s) | 1679 |
| NcoI | CCATGG | 1 cut(s) | 1833 |
| NlaIV | GGNNCC | 2 cut(s) | 319, 972 |
| NmeAIII | GCCGAG | 1 cut(s) | 73 |
| NmuCI | GTSAC | 4 cut(s) | 427, 493, 1343, 1732 |
| NruI | TCGCGA | 1 cut(s) | 114 |
| NsbI | TGCGCA | 1 cut(s) | 827 |
| NspI | RCATGY | 2 cut(s) | 1819, 2062 |
| PaeR7I | CTCGAG | 1 cut(s) | 1990 |
| PagI | TCATGA | 2 cut(s) | 784, 1279 |
| PciI | ACATGT | 1 cut(s) | 1815 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1905 |
| PctI | GAATGC | 3 cut(s) | 38, 610, 1250 |
| PdmI | GAANNNNTTC | 1 cut(s) | 914 |
| PfeI | GAWTC | 5 cut(s) | 156, 390, 1276, 1592, 1959 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 888 |
| PkrI | GCNGC | 6 cut(s) | 519, 825, 1727, 1926, 1929, 2080 |
| PleI | GAGTC | 4 cut(s) | 171, 475, 1094, 1709 |
| PpsI | GAGTC | 4 cut(s) | 171, 475, 1094, 1709 |
| PpuMI | RGGWCCY | 1 cut(s) | 1915 |
| PscI | ACATGT | 1 cut(s) | 1815 |
| Psp124BI | GAGCTC | 2 cut(s) | 120, 1062 |
| Psp5II | RGGWCCY | 1 cut(s) | 1915 |
| Psp6I | CCWGG | 2 cut(s) | 42, 992 |
| PspFI | CCCAGC | 1 cut(s) | 2014 |
| PspGI | CCWGG | 2 cut(s) | 42, 992 |
| PspN4I | GGNNCC | 2 cut(s) | 319, 972 |
| PspPI | GGNCC | 2 cut(s) | 1390, 1915 |
| PspPPI | RGGWCCY | 1 cut(s) | 1915 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 523, 555 |
| PstNI | CAGNNNCTG | 1 cut(s) | 64 |
| PsuI | RGATCY | 1 cut(s) | 1642 |
| PvuII | CAGCTG | 1 cut(s) | 2081 |
| RruI | TCGCGA | 1 cut(s) | 114 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1196 |
| SacI | GAGCTC | 2 cut(s) | 120, 1062 |
| SalI | GTCGAC | 1 cut(s) | 1712 |
| SaqAI | TTAA | 4 cut(s) | 12, 507, 1014, 1086 |
| SatI | GCNGC | 6 cut(s) | 518, 824, 1726, 1925, 1928, 2079 |
| Sau96I | GGNCC | 2 cut(s) | 1390, 1915 |
| SchI | GAGTC | 4 cut(s) | 172, 476, 1094, 1709 |
| ScrFI | CCNGG | 3 cut(s) | 44, 994, 1679 |
| SduI | GDGCHC | 4 cut(s) | 120, 302, 973, 1062 |
| SfcI | CTRYAG | 1 cut(s) | 88 |
| Sfr274I | CTCGAG | 1 cut(s) | 1990 |
| SinI | GGWCC | 1 cut(s) | 1915 |
| SlaI | CTCGAG | 1 cut(s) | 1990 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1196 |
| SmlI | CTYRAG | 3 cut(s) | 551, 1463, 1990 |
| SmoI | CTYRAG | 3 cut(s) | 551, 1463, 1990 |
| Sse9I | AATT | 8 cut(s) | 127, 303, 617, 938, 1503, 1526, 1707, 1995 |
| SsiI | CCGC | 4 cut(s) | 5, 258, 413, 2054 |
| SstI | GAGCTC | 2 cut(s) | 120, 1062 |
| StyD4I | CCNGG | 3 cut(s) | 42, 992, 1677 |
| StyI | CCWWGG | 3 cut(s) | 321, 846, 1833 |
| TaaI | ACNGT | 4 cut(s) | 378, 493, 1052, 2044 |
| TaiI | ACGT | 3 cut(s) | 35, 86, 462 |
| TaqI | TCGA | 7 cut(s) | 159, 987, 1256, 1584, 1713, 1991, 2028 |
| TasI | AATT | 8 cut(s) | 127, 303, 617, 938, 1503, 1526, 1707, 1995 |
| TfiI | GAWTC | 5 cut(s) | 156, 390, 1276, 1592, 1959 |
| Tru1I | TTAA | 4 cut(s) | 12, 507, 1014, 1086 |
| Tru9I | TTAA | 4 cut(s) | 12, 507, 1014, 1086 |
| TscAI | CASTG | 4 cut(s) | 496, 679, 1440, 1741 |
| TseFI | GTSAC | 4 cut(s) | 427, 493, 1343, 1732 |
| TseI | GCWGC | 6 cut(s) | 517, 823, 1725, 1924, 1927, 2078 |
| Tsp45I | GTSAC | 4 cut(s) | 427, 493, 1343, 1732 |
| TspGWI | ACGGA | 2 cut(s) | 185, 1897 |
| TspRI | CASTG | 4 cut(s) | 496, 679, 1440, 1741 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 888 |
| VpaK11BI | GGWCC | 1 cut(s) | 1915 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 554 |
| XapI | RAATTY | 3 cut(s) | 617, 938, 1995 |
| XceI | RCATGY | 2 cut(s) | 1819, 2062 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 280, 328 |
| XhoI | CTCGAG | 1 cut(s) | 1990 |
| XmiI | GTMKAC | 1 cut(s) | 1713 |
| XmnI | GAANNNNTTC | 1 cut(s) | 914 |