Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0000617

Physical Location & Seq

Genomic Coordinates Reverse (-)

11037702 .. 11039412

Gene Structure 1711 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027373

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGCCTAAGGAGTCGGTTTCACGGATGCTCACTCAAGCAAAGCCATTTGTGGCTGTGATTCTGATGCAATTTGGGTTAGCAGGAATGTCCATAATTACAAAGTTTGCTCTAAACCAAGGGATGAGCCAACATGTATTAGTTGTCTATAGGCACGCTGTTGCCTTTGTTTTTATAACTCCTTTTGCCCTTGTCTTTGATAGGAAAATAAGGCCAAAGATGACCCTTTCAGTCTTTTTCAAGATTGTGATCCTCGGCCTATTAGAGCCTGTAATCGACGGGAACTTGTTCTATACTGGGATGAAGCTGACAACAGCAACATTTACAATGGCCATGTGCAATGTTGTTCCTGCCTTTGCATTCATAATGGCTTGGATTTTCAGGCTTGAGATGGTTGATATTAGAAGACTTCACAGCCAGGCAAAGATATTGGGGACCATAGTGACTGTGGGAGGAGCTATGCTTATGACTCTGGTCAATGGACCCATGTTGACTCTTCCATGGACTAGAAGCAGTAGCCATCATCAAACTACAAGTTCCACAAATAGCCAAGATCCCATAAAGGGTGCTCTCATGATTACAGCTGGATGTTTCTGCTGGGCTATATACCCTGCTGAGCTCTCCCTAACAGCTTGGATATGTTTGATGGGTGCGTTGGAAGGTACCGTGGCAGCTCTAGCTTTTGAGTGGCATAACCCTGCAGCTTGGGCCATACACTTGGATTCTAAGTTGTTAGCTGCTGTTTACGGTGGAGTAATATGTTCTGGAGTGACTTATTATATTCAGGGGATGGTAATGAAGGAAAGAGGACCTGTTTTTGTGACTGCATTTAATCCCCTATGCATGGTCATTGTAGCCATTTTGAGCTCCATCGCCTTGTCCGAAATACTGTACTTGGGAAGGGTAATGGGAGCAATGGTTATAGTAATTGGCCTGTATATGGTCCTCTGGGGTAAAAGTAAGGATCCTCTTCCACAACCAGATTCAGAAGCAGCTGAAGATAAAGTAGCTACAACAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001504 GO:0001505 GO:0003006 GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006835 GO:0006836 GO:0006855 GO:0006865 GO:0006868 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008324 GO:0008509 GO:0008514 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009791 GO:0009987 GO:0010033 GO:0010154 GO:0015075 GO:0015171 GO:0015175 GO:0015179 GO:0015186 GO:0015238 GO:0015318 GO:0015711 GO:0015800 GO:0015804 GO:0015807 GO:0015813 GO:0015849 GO:0015893 GO:0016020 GO:0016021 GO:0022414 GO:0022857 GO:0023052 GO:0031224 GO:0031226 GO:0032501 GO:0032502 GO:0032870 GO:0034220 GO:0042221 GO:0042493 GO:0042592 GO:0043090 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046942 GO:0046943 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0051938 GO:0055081 GO:0055085 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071702 GO:0071705 GO:0071944 GO:0080144 GO:0089718 GO:0098655 GO:0098656 GO:0098657 GO:0098712 GO:0098739 GO:0099568 GO:1902475 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

340

Amino Acids

37.07

Weight (kDa)

9.0

Isoelectric Point (pI)

33.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	18 - 156	2.5e-08	EamA-like transporter family
EamA	PF00892	187 - 315	7.9e-06	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000617)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39510
fragaria_vesca	FvH4_7g21440 FvH4_7g21790 FvH4_7g21820
malus_domestica	MD01G1122900.v1.1 MD01G1123000.v1.1 MD02G1281100.v1.1 MD02G1281200.v1.1 MD07G1192500.v1.1 MD07G1192600.v1.1
prunus_persica	Prupe.2G230500_v2.0.a1 Prupe.2G230600_v2.0.a1 Prupe.2G230600_v2.0.a1
pyrus_communis	pycom01g14990 pycom07g18200 pycom07g18220
rosa_chinensis	RchiOBHm_Chr1g0337261 RchiOBHm_Chr1g0337271 RchiOBHm_Chr1g0366051 RchiOBHm_Chr1g0366071 RchiOBHm_Chr1g0366081 RchiOBHm_Chr1g0366091 RchiOBHm_Chr1g0366101 RchiOBHm_Chr2g0101901 RchiOBHm_Chr7g0232741 RchiOBHm_Chr7g0232791 RchiOBHm_Chr7g0232801 RchiOBHm_Chr7g0232841
rosa_laevigata	RLG00000017109 RLG00000017110 RLG00000017111 RLG00000017114 RLG00000027368 RLG00000027369 RLG00000027370 RLG00000027371 RLG00000027373 RLG00000029288
rosa_multiflora	Rmu_sc0000067.1_g000001 Rmu_sc0003987.1_g000005 Rmu_sc0003987.1_g000006 Rmu_sc0003987.1_g000010 Rmu_sc0004469.1_g000021 Rmu_sc0005795.1_g000006 Rmu_sc0017606.1_g000009 Rmu_sc0026503.1_g000001
rosa_roxburghii	Rroxscaffold_3G00227870 Rroxscaffold_3G00227880 Rroxscaffold_4G00290500 Rroxscaffold_4G00290510 Rroxscaffold_4G00290520 Rroxscaffold_4G00290530 Rroxscaffold_4G00290560 Rroxscaffold_4G00314630
rosa_rugosa	Rorug01G0133800.1 Rorug01G0133900.1 Rorug01G0326800 Rorug01G0326900 Rorug01G0327100 Rorug01G0327200 Rorug01G0327300
rosa_samantha	Rh1AG153100 Rh1AG335500 Rh1BG117400 Rh1BG122400 Rh1BG295900 Rh1BG296200 Rh1BG296400 Rh1BG296500 Rh1BG296600 Rh1BG296700 Rh2BG156100 Rh2CG156200 Rh7CG449900
rosa_wichuraiana	Rw1G012530 Rw1G029740 Rw1G029750 Rw1G029760 Rw2G011770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	173
Acc65I	GGTACC	1 cut(s)	659
AccB1I	GGYRCC	1 cut(s)	659
AclWI	GGATC	4 cut(s)	241, 545, 956, 969
AcoI	YGGCCR	1 cut(s)	327
AcuI	CTGAAG	1 cut(s)	1014
AfaI	GTAC	2 cut(s)	661, 890
AfiI	CCNNNNNNNGG	3 cut(s)	560, 841, 937
AflIII	ACRYGT	1 cut(s)	130
AgsI	TTSAA	1 cut(s)	238
AjnI	CCWGG	1 cut(s)	414
Alw21I	GWGCWC	3 cut(s)	568, 618, 866
AlwI	GGATC	4 cut(s)	241, 545, 956, 969
AoxI	GGCC	5 cut(s)	209, 253, 327, 705, 928
ApeKI	GCWGC	4 cut(s)	668, 698, 734, 989
Asp700I	GAANNNNTTC	1 cut(s)	284
Asp718I	GGTACC	1 cut(s)	659
AspS9I	GGNCC	5 cut(s)	432, 479, 705, 806, 940
AvaII	GGWCC	4 cut(s)	432, 479, 806, 940
AxyI	CCTNAGG	1 cut(s)	6
BalI	TGGCCA	1 cut(s)	329
BamHI	GGATCC	1 cut(s)	961
BanI	GGYRCC	1 cut(s)	659
BanII	GRGCYC	2 cut(s)	618, 866
BbsI	GAAGAC	1 cut(s)	409
Bbv12I	GWGCWC	3 cut(s)	568, 618, 866
BbvI	GCAGC	4 cut(s)	680, 710, 721, 1001
BccI	CCATC	5 cut(s)	382, 525, 637, 781, 875
BciT130I	CCWGG	1 cut(s)	416
BfaI	CTAG	2 cut(s)	504, 674
BfmI	CTRYAG	2 cut(s)	145, 696
BisI	GCNGC	4 cut(s)	669, 699, 735, 990
BlpI	GCTNAGC	1 cut(s)	612
BlsI	GCNGC	4 cut(s)	670, 700, 736, 991
Bme1390I	CCNGG	1 cut(s)	416
Bme18I	GGWCC	4 cut(s)	432, 479, 806, 940
BmgT120I	GGNCC	5 cut(s)	432, 479, 705, 806, 940
BmiI	GGNNCC	4 cut(s)	433, 481, 661, 963
BmrFI	CCNGG	1 cut(s)	416
BmrI	ACTGGG	1 cut(s)	303
BmsI	GCATC	2 cut(s)	15, 54
BmuI	ACTGGG	1 cut(s)	303
BoxI	GACNNNNGTC	1 cut(s)	470
BpiI	GAAGAC	1 cut(s)	409
BpmI	CTGGAG	1 cut(s)	783
Bpu1102I	GCTNAGC	1 cut(s)	612
BpuEI	CTTGAG	2 cut(s)	18, 404
BsaBI	GATNNNNATC	1 cut(s)	245
BsaJI	CCNNGG	4 cut(s)	115, 250, 497, 663
BsaXI	ACNNNNNCTCC	2 cut(s)	900, 930
Bsc4I	CCNNNNNNNGG	3 cut(s)	560, 841, 937
Bse1I	ACTGG	1 cut(s)	298
Bse21I	CCTNAGG	1 cut(s)	6
Bse3DI	GCAATG	2 cut(s)	343, 918
Bse8I	GATNNNNATC	1 cut(s)	245
BseBI	CCWGG	1 cut(s)	416
BseDI	CCNNGG	4 cut(s)	115, 250, 497, 663
BseGI	GGATG	5 cut(s)	30, 126, 303, 590, 792
BseJI	GATNNNNATC	1 cut(s)	245
BseLI	CCNNNNNNNGG	3 cut(s)	560, 841, 937
BseMI	GCAATG	2 cut(s)	343, 918
BseMII	CTCAG	1 cut(s)	603
BseNI	ACTGG	1 cut(s)	298
BseRI	GAGGAG	1 cut(s)	465
BseXI	GCAGC	4 cut(s)	680, 710, 721, 1001
BseYI	CCCAGC	1 cut(s)	594
BshFI	GGCC	5 cut(s)	211, 255, 329, 707, 930
BshNI	GGYRCC	1 cut(s)	659
BsiHKAI	GWGCWC	3 cut(s)	568, 618, 866
BslFI	GGGAC	1 cut(s)	445
BslI	CCNNNNNNNGG	3 cut(s)	560, 841, 937
BsmFI	GGGAC	1 cut(s)	445
BsmI	GAATGC	1 cut(s)	356
BsnI	GGCC	5 cut(s)	211, 255, 329, 707, 930
Bsp1286I	GDGCHC	3 cut(s)	568, 618, 866
Bsp143I	GATC	3 cut(s)	246, 550, 961
Bsp1720I	GCTNAGC	1 cut(s)	612
Bsp19I	CCATGG	1 cut(s)	497
BspANI	GGCC	5 cut(s)	211, 255, 329, 707, 930
BspCNI	CTCAG	1 cut(s)	604
BspHI	TCATGA	1 cut(s)	570
BspLI	GGNNCC	4 cut(s)	433, 481, 661, 963
BspMAI	CTGCAG	1 cut(s)	700
BspPI	GGATC	4 cut(s)	241, 545, 956, 969
BspT107I	GGYRCC	1 cut(s)	659
BsrDI	GCAATG	2 cut(s)	343, 918
BsrI	ACTGG	1 cut(s)	298
BssECI	CCNNGG	4 cut(s)	115, 250, 497, 663
BssMI	GATC	3 cut(s)	246, 550, 961
BssT1I	CCWWGG	2 cut(s)	115, 497
Bst2UI	CCWGG	1 cut(s)	416
Bst4CI	ACNGT	5 cut(s)	445, 664, 746, 888, 1015
Bst6I	CTCTTC	2 cut(s)	498, 972
BstC8I	GCNNGC	1 cut(s)	153
BstDEI	CTNAG	3 cut(s)	6, 612, 723
BstDSI	CCRYGG	2 cut(s)	497, 663
BstF5I	GGATG	5 cut(s)	30, 126, 303, 590, 792
BstKTI	GATC	3 cut(s)	249, 553, 964
BstMBI	GATC	3 cut(s)	246, 550, 961
BstMWI	GCNNNNNNNGC	2 cut(s)	674, 704
BstNI	CCWGG	1 cut(s)	416
BstNSI	RCATGY	1 cut(s)	134
BstPAI	GACNNNNGTC	1 cut(s)	470
BstSCI	CCNGG	1 cut(s)	414
BstSFI	CTRYAG	2 cut(s)	145, 696
BstV1I	GCAGC	4 cut(s)	680, 710, 721, 1001
BstV2I	GAAGAC	1 cut(s)	409
BstX2I	RGATCY	2 cut(s)	550, 961
BstXI	CCANNNNNNTGG	1 cut(s)	715
BstYI	RGATCY	2 cut(s)	550, 961
Bsu36I	CCTNAGG	1 cut(s)	6
BsuRI	GGCC	5 cut(s)	211, 255, 329, 707, 930
BtgI	CCRYGG	2 cut(s)	497, 663
BtgZI	GCGATG	1 cut(s)	853
BtsCI	GGATG	5 cut(s)	30, 126, 303, 590, 792
Cac8I	GCNNGC	1 cut(s)	153
CciI	TCATGA	1 cut(s)	570
Cfr13I	GGNCC	5 cut(s)	432, 479, 705, 806, 940
Csp6I	GTAC	2 cut(s)	660, 889
CviAII	CATG	6 cut(s)	131, 331, 484, 498, 571, 841
CviQI	GTAC	2 cut(s)	660, 889
DdeI	CTNAG	3 cut(s)	6, 612, 723
DpnI	GATC	3 cut(s)	248, 552, 963
DpnII	GATC	3 cut(s)	246, 550, 961
EaeI	YGGCCR	1 cut(s)	327
Eam1104I	CTCTTC	2 cut(s)	498, 972
EarI	CTCTTC	2 cut(s)	498, 972
Ecl136II	GAGCTC	2 cut(s)	616, 864
Eco130I	CCWWGG	2 cut(s)	115, 497
Eco24I	GRGCYC	2 cut(s)	618, 866
Eco47I	GGWCC	4 cut(s)	432, 479, 806, 940
Eco53kI	GAGCTC	2 cut(s)	616, 864
Eco57I	CTGAAG	1 cut(s)	1014
Eco81I	CCTNAGG	1 cut(s)	6
EcoICRI	GAGCTC	2 cut(s)	616, 864
EcoO109I	RGGNCCY	1 cut(s)	806
EcoRII	CCWGG	1 cut(s)	414
EcoT14I	CCWWGG	2 cut(s)	115, 497
EcoT22I	ATGCAT	1 cut(s)	842
EcoT38I	GRGCYC	2 cut(s)	618, 866
ErhI	CCWWGG	2 cut(s)	115, 497
FaeI	CATG	6 cut(s)	134, 334, 487, 501, 574, 844
FaqI	GGGAC	1 cut(s)	445
FatI	CATG	6 cut(s)	130, 330, 483, 497, 570, 840
Fnu4HI	GCNGC	4 cut(s)	669, 699, 735, 990
FokI	GGATG	5 cut(s)	37, 133, 310, 597, 799
FriOI	GRGCYC	2 cut(s)	618, 866
Fsp4HI	GCNGC	4 cut(s)	669, 699, 735, 990
FspBI	CTAG	2 cut(s)	504, 674
GluI	GCNGC	4 cut(s)	669, 699, 735, 990
GsaI	CCCAGC	1 cut(s)	598
GsuI	CTGGAG	1 cut(s)	783
HaeIII	GGCC	5 cut(s)	211, 255, 329, 707, 930
Hin1II	CATG	6 cut(s)	134, 334, 487, 501, 574, 844
HincII	GTYRAC	1 cut(s)	489
HindII	GTYRAC	1 cut(s)	489
HinfI	GANTC	6 cut(s)	11, 58, 466, 490, 719, 980
Hpy166II	GTNNAC	2 cut(s)	489, 742
Hpy188I	TCNGA	3 cut(s)	63, 880, 985
Hpy188III	TCNNGA	3 cut(s)	238, 571, 762
Hpy8I	GTNNAC	2 cut(s)	489, 742
Hpy99I	CGWCG	1 cut(s)	278
HpyAV	CCTTC	3 cut(s)	650, 790, 891
HpyCH4III	ACNGT	5 cut(s)	445, 664, 746, 888, 1015
HpyCH4V	TGCA	6 cut(s)	67, 336, 356, 698, 824, 840
HpyF10VI	GCNNNNNNNGC	2 cut(s)	674, 704
HpyF3I	CTNAG	3 cut(s)	6, 612, 723
Hsp92II	CATG	6 cut(s)	134, 334, 487, 501, 574, 844
KpnI	GGTACC	1 cut(s)	663
Kzo9I	GATC	3 cut(s)	246, 550, 961
LmnI	GCTCC	3 cut(s)	452, 869, 908
Lsp1109I	GCAGC	4 cut(s)	680, 710, 721, 1001
LweI	GCATC	2 cut(s)	15, 54
MaeI	CTAG	2 cut(s)	504, 674
MaeIII	GTNAC	3 cut(s)	439, 766, 817
MalI	GATC	3 cut(s)	248, 552, 963
MboI	GATC	3 cut(s)	246, 550, 961
MboII	GAAGA	4 cut(s)	414, 485, 959, 1007
MflI	RGATCY	2 cut(s)	550, 961
MhlI	GDGCHC	3 cut(s)	568, 618, 866
MlsI	TGGCCA	1 cut(s)	329
MluCI	AATT	3 cut(s)	68, 93, 924
MluNI	TGGCCA	1 cut(s)	329
MlyI	GAGTC	3 cut(s)	20, 460, 484
MmeI	TCCRAC	1 cut(s)	633
MnlI	CCTC	5 cut(s)	260, 443, 797, 953, 975
Mox20I	TGGCCA	1 cut(s)	329
Mph1103I	ATGCAT	1 cut(s)	842
MroXI	GAANNNNTTC	1 cut(s)	284
MscI	TGGCCA	1 cut(s)	329
MseI	TTAA	2 cut(s)	828, 1018
Msp20I	TGGCCA	1 cut(s)	329
MspA1I	CMGCKG	2 cut(s)	581, 992
MspR9I	CCNGG	1 cut(s)	416
Mva1269I	GAATGC	1 cut(s)	356
MvaI	CCWGG	1 cut(s)	416
MwoI	GCNNNNNNNGC	2 cut(s)	674, 704
NcoI	CCATGG	1 cut(s)	497
NdeII	GATC	3 cut(s)	246, 550, 961
NlaIII	CATG	6 cut(s)	134, 334, 487, 501, 574, 844
NlaIV	GGNNCC	4 cut(s)	433, 481, 661, 963
NmeAIII	GCCGAG	1 cut(s)	231
NmuCI	GTSAC	3 cut(s)	439, 766, 817
NsiI	ATGCAT	1 cut(s)	842
NspI	RCATGY	1 cut(s)	134
PagI	TCATGA	1 cut(s)	570
PciI	ACATGT	1 cut(s)	130
PcsI	WCGNNNNNNNCGW	1 cut(s)	876
PctI	GAATGC	1 cut(s)	356
PdmI	GAANNNNTTC	1 cut(s)	284
PfeI	GAWTC	3 cut(s)	58, 719, 980
PkrI	GCNGC	4 cut(s)	670, 700, 736, 991
PleI	GAGTC	3 cut(s)	19, 460, 484
PpsI	GAGTC	3 cut(s)	19, 460, 484
PpuMI	RGGWCCY	1 cut(s)	806
PscI	ACATGT	1 cut(s)	130
PshAI	GACNNNNGTC	1 cut(s)	470
PsiI	TTATAA	1 cut(s)	173
Psp124BI	GAGCTC	2 cut(s)	618, 866
Psp5II	RGGWCCY	1 cut(s)	806
Psp6I	CCWGG	1 cut(s)	414
PspFI	CCCAGC	1 cut(s)	594
PspGI	CCWGG	1 cut(s)	414
PspN4I	GGNNCC	4 cut(s)	433, 481, 661, 963
PspPI	GGNCC	5 cut(s)	432, 479, 705, 806, 940
PspPPI	RGGWCCY	1 cut(s)	806
PstI	CTGCAG	1 cut(s)	700
PsuI	RGATCY	2 cut(s)	550, 961
PvuII	CAGCTG	2 cut(s)	581, 992
RsaI	GTAC	2 cut(s)	661, 890
RsaNI	GTAC	2 cut(s)	660, 889
SacI	GAGCTC	2 cut(s)	618, 866
SaqAI	TTAA	2 cut(s)	828, 1018
SatI	GCNGC	4 cut(s)	669, 699, 735, 990
Sau3AI	GATC	3 cut(s)	246, 550, 961
Sau96I	GGNCC	5 cut(s)	432, 479, 705, 806, 940
SchI	GAGTC	3 cut(s)	20, 460, 484
ScrFI	CCNGG	1 cut(s)	416
SduI	GDGCHC	3 cut(s)	568, 618, 866
SfaNI	GCATC	2 cut(s)	15, 54
SfcI	CTRYAG	2 cut(s)	145, 696
SinI	GGWCC	4 cut(s)	432, 479, 806, 940
SmlI	CTYRAG	2 cut(s)	33, 383
SmoI	CTYRAG	2 cut(s)	33, 383
Sse9I	AATT	3 cut(s)	68, 93, 924
SspMI	CTAG	2 cut(s)	504, 674
SstI	GAGCTC	2 cut(s)	618, 866
StyD4I	CCNGG	1 cut(s)	414
StyI	CCWWGG	2 cut(s)	115, 497
TaaI	ACNGT	5 cut(s)	445, 664, 746, 888, 1015
TaqI	TCGA	1 cut(s)	273
TasI	AATT	3 cut(s)	68, 93, 924
TatI	WGTACW	1 cut(s)	888
TfiI	GAWTC	3 cut(s)	58, 719, 980
Tru1I	TTAA	2 cut(s)	828, 1018
Tru9I	TTAA	2 cut(s)	828, 1018
TseFI	GTSAC	3 cut(s)	439, 766, 817
TseI	GCWGC	4 cut(s)	668, 698, 734, 989
Tsp45I	GTSAC	3 cut(s)	439, 766, 817
TspDTI	ATGAA	3 cut(s)	314, 349, 809
TspGWI	ACGGA	1 cut(s)	37
VpaK11BI	GGWCC	4 cut(s)	432, 479, 806, 940
XceI	RCATGY	1 cut(s)	134
XcmI	CCANNNNNNNNNTGG	1 cut(s)	442
XmnI	GAANNNNTTC	1 cut(s)	284
XspI	CTAG	2 cut(s)	504, 674
Zsp2I	ATGCAT	1 cut(s)	842

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.