Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0000617

Physical Location & Seq

Genomic Coordinates Reverse (-)

44677783 .. 44683911

Gene Structure 6129 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0327200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1164 bp

ATGGGTCGTTTTTTCACGGCGGCGGCGTTGGTGGTGGTGTTGCTTCCGATCTGGGTTTTGGCGGCGGCGAAGCACCATGAAGAGGAACCAATGAGGCTGAGGTTTGGGGGAGATGGGCAGTTTAAGATACTGCAAGTGGCGGACATGCACTATGCCGATGGGAAAACGACGCCGTGTGAGGATGTGCTGCCGTCACAGGTGGCGGGTTGCTCTGACCTTAATACGACGGCGTTCGTTCAGCGGATGATTCAGGCTGAGAAGCCCAATTTCATTGTTTTCACCGGAGATAACATCTATGGGTTTGATGCTACGGATGCTGCTAAATCAATGGATGCAGCATTTGCCCCAGCAATCGCGTCCAACATTCCATGGGCAGCTGTTTTGGGGAACCATGATCAGGAGTCGGACCTTTCGAGGGAAGGGGTAATGAAGCACGTAGTTAAGTATAAAAGCACCCTGGCTCAACTCAATCCTTCGGATCAAGATGTTATTGATGGTTTCGGGAACTATAACCTGGAGGTAGGTGGAGTTCAGGGTACTGGTTTTGAAAACAAATCGGTTCTCAATCTATACTTCCTTGACAGTGGAGATTACTCCACAGTTCCCTCTATCCCTGGCTATGGTTGGATCAAACCCTCCCAGCAATTTTGGTTCCAACAAACTTCAGCAAAGCTAAAGAAAGCATACATGAAAAAGCCAGAGGCGCAGAAAACACCTGCTCCAGGGCTAGCATACTTTCACATACCATTACCAGAATATGCTAGTTTTGACTCATCAAACTTCACAGGAGTGAGGCAAGAAGGCATTAGCTCTGCCTCCGTGAACTCTGGCTTCTTCACAACCCTGGTTGAAGCAGGCGATGTAAAGGCAACTTTCACAGGCCATGATCACCTCAATGACTTCTGTGGGAAGCTAACTGGCATAAACCTTTGTTATGCTGGGGGATTCGGATACCATGCATATGGGAAGGCAGGATGGGATAGGAGAGCAAGGGTGGTAGCAGCAACCTTAGAGAAAACAAACACTGGAGGTTGGGGAGCAGTCAAGTCAATCAAAACATGGAAACGCCTCGACGATCAGCACCTCAGTACTATCAATAGTGAGGTTCTCTGGACCAAGAGTTCTGCGGGTGCACGTAGAAAGAAACCCATTGGCGGTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001504 GO:0001505 GO:0003006 GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006820 GO:0006835 GO:0006836 GO:0006855 GO:0006865 GO:0006868 GO:0007275 GO:0008150 GO:0008324 GO:0008509 GO:0008514 GO:0009791 GO:0010154 GO:0015075 GO:0015171 GO:0015175 GO:0015179 GO:0015186 GO:0015238 GO:0015318 GO:0015711 GO:0015800 GO:0015804 GO:0015807 GO:0015813 GO:0015849 GO:0015893 GO:0016020 GO:0022414 GO:0022857 GO:0032501 GO:0032502 GO:0034220 GO:0042221 GO:0042493 GO:0042592 GO:0043090 GO:0044464 GO:0046942 GO:0046943 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051938 GO:0055081 GO:0055085 GO:0061458 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071944 GO:0080144 GO:0089718 GO:0098655 GO:0098656 GO:0098657 GO:0098712 GO:0098739 GO:1902475 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

387

Amino Acids

42.13

Weight (kDa)

7.14

Isoelectric Point (pI)

33.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Metallophos	PF00149	41 - 297	9.9e-14	Calcineurin-like phosphoesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000617)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39510
fragaria_vesca	FvH4_7g21440 FvH4_7g21790 FvH4_7g21820
malus_domestica	MD01G1122900.v1.1 MD01G1123000.v1.1 MD02G1281100.v1.1 MD02G1281200.v1.1 MD07G1192500.v1.1 MD07G1192600.v1.1
prunus_persica	Prupe.2G230500_v2.0.a1 Prupe.2G230600_v2.0.a1 Prupe.2G230600_v2.0.a1
pyrus_communis	pycom01g14990 pycom07g18200 pycom07g18220
rosa_chinensis	RchiOBHm_Chr1g0337261 RchiOBHm_Chr1g0337271 RchiOBHm_Chr1g0366051 RchiOBHm_Chr1g0366071 RchiOBHm_Chr1g0366081 RchiOBHm_Chr1g0366091 RchiOBHm_Chr1g0366101 RchiOBHm_Chr2g0101901 RchiOBHm_Chr7g0232741 RchiOBHm_Chr7g0232791 RchiOBHm_Chr7g0232801 RchiOBHm_Chr7g0232841
rosa_laevigata	RLG00000017109 RLG00000017110 RLG00000017111 RLG00000017114 RLG00000027368 RLG00000027369 RLG00000027370 RLG00000027371 RLG00000027373 RLG00000029288
rosa_multiflora	Rmu_sc0000067.1_g000001 Rmu_sc0003987.1_g000005 Rmu_sc0003987.1_g000006 Rmu_sc0003987.1_g000010 Rmu_sc0004469.1_g000021 Rmu_sc0005795.1_g000006 Rmu_sc0017606.1_g000009 Rmu_sc0026503.1_g000001
rosa_roxburghii	Rroxscaffold_3G00227870 Rroxscaffold_3G00227880 Rroxscaffold_4G00290500 Rroxscaffold_4G00290510 Rroxscaffold_4G00290520 Rroxscaffold_4G00290530 Rroxscaffold_4G00290560 Rroxscaffold_4G00314630
rosa_rugosa	Rorug01G0133800.1 Rorug01G0133900.1 Rorug01G0326800 Rorug01G0326900 Rorug01G0327100 Rorug01G0327200 Rorug01G0327300
rosa_samantha	Rh1AG153100 Rh1AG335500 Rh1BG117400 Rh1BG122400 Rh1BG295900 Rh1BG296200 Rh1BG296400 Rh1BG296500 Rh1BG296600 Rh1BG296700 Rh2BG156100 Rh2CG156200 Rh7CG449900
rosa_wichuraiana	Rw1G012530 Rw1G029740 Rw1G029750 Rw1G029760 Rw2G011770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	724
Acc36I	ACCTGC	1 cut(s)	724
AccII	CGCG	1 cut(s)	356
AciI	CCGC	9 cut(s)	20, 23, 62, 65, 140, 203, 241, 1127, 1155
AclWI	GGATC	2 cut(s)	486, 635
AcuI	CTGAAG	1 cut(s)	648
AcyI	GRCGYC	1 cut(s)	170
AfaI	GTAC	2 cut(s)	538, 1090
AfiI	CCNNNNNNNGG	6 cut(s)	82, 397, 415, 620, 722, 1154
AgsI	TTSAA	2 cut(s)	548, 851
AjnI	CCWGG	5 cut(s)	456, 513, 613, 721, 843
AleI	CACNNNNGTG	1 cut(s)	788
AloI	GAACNNNNNNTCC	2 cut(s)	1105, 1137
AluBI	AGCT	4 cut(s)	377, 673, 810, 913
AluI	AGCT	4 cut(s)	377, 673, 810, 913
Alw21I	GWGCWC	1 cut(s)	1135
Alw44I	GTGCAC	1 cut(s)	1131
AlwI	GGATC	2 cut(s)	486, 635
AoxI	GGCC	1 cut(s)	880
ApaLI	GTGCAC	1 cut(s)	1131
ApeKI	GCWGC	5 cut(s)	187, 317, 335, 374, 1001
AspLEI	GCGC	1 cut(s)	706
AspS9I	GGNCC	3 cut(s)	406, 1113, 1157
AsuHPI	GGTGA	2 cut(s)	271, 881
AsuNHI	GCTAGC	1 cut(s)	727
AvaII	GGWCC	3 cut(s)	406, 1113, 1157
BaeGI	GKGCMC	1 cut(s)	1135
Bbv12I	GWGCWC	1 cut(s)	1135
BbvCI	CCTCAGC	1 cut(s)	98
BbvI	GCAGC	5 cut(s)	174, 304, 347, 386, 1013
BccI	CCATC	4 cut(s)	107, 152, 488, 969
BceAI	ACGGC	4 cut(s)	33, 157, 175, 243
BciT130I	CCWGG	5 cut(s)	458, 515, 615, 723, 845
BciVI	GTATCC	1 cut(s)	944
BclI	TGATCA	2 cut(s)	394, 886
BfaI	CTAG	2 cut(s)	728, 762
BfuAI	ACCTGC	1 cut(s)	724
BfuI	GTATCC	1 cut(s)	944
BisI	GCNGC	9 cut(s)	21, 24, 63, 66, 188, 318, 336, 375, 1002
BlsI	GCNGC	9 cut(s)	22, 25, 64, 67, 189, 319, 337, 376, 1003
BmcAI	AGTACT	1 cut(s)	1090
Bme1390I	CCNGG	5 cut(s)	458, 515, 615, 723, 845
Bme18I	GGWCC	3 cut(s)	406, 1113, 1157
BmgT120I	GGNCC	3 cut(s)	406, 1113, 1157
BmiI	GGNNCC	3 cut(s)	87, 389, 653
BmrFI	CCNGG	5 cut(s)	458, 515, 615, 723, 845
BmsI	GCATC	3 cut(s)	295, 304, 322
BmtI	GCTAGC	1 cut(s)	731
BpmI	CTGGAG	3 cut(s)	536, 705, 1047
Bpu10I	CCTNAGC	1 cut(s)	98
BsaAI	YACGTR	2 cut(s)	436, 1136
BsaHI	GRCGYC	1 cut(s)	170
BsaJI	CCNNGG	5 cut(s)	368, 456, 613, 722, 843
BsaWI	WCCGGW	1 cut(s)	281
BsaXI	ACNNNNNCTCC	2 cut(s)	703, 733
Bsc4I	CCNNNNNNNGG	6 cut(s)	82, 397, 415, 620, 722, 1154
Bse1I	ACTGG	3 cut(s)	544, 922, 1030
BseBI	CCWGG	5 cut(s)	458, 515, 615, 723, 845
BseDI	CCNNGG	5 cut(s)	368, 456, 613, 722, 843
BseGI	GGATG	5 cut(s)	187, 249, 319, 337, 980
BseLI	CCNNNNNNNGG	6 cut(s)	82, 397, 415, 620, 722, 1154
BseMII	CTCAG	3 cut(s)	89, 246, 1099
BseNI	ACTGG	3 cut(s)	544, 922, 1030
BseSI	GKGCMC	1 cut(s)	1135
BseXI	GCAGC	5 cut(s)	174, 304, 347, 386, 1013
BseYI	CCCAGC	3 cut(s)	346, 639, 938
Bsh1236I	CGCG	1 cut(s)	356
BshFI	GGCC	1 cut(s)	882
BsiHKAI	GWGCWC	1 cut(s)	1135
BsiSI	CCGG	1 cut(s)	282
BslI	CCNNNNNNNGG	6 cut(s)	82, 397, 415, 620, 722, 1154
BsnI	GGCC	1 cut(s)	882
Bsp1286I	GDGCHC	1 cut(s)	1135
Bsp143I	GATC	6 cut(s)	48, 394, 478, 627, 886, 1075
Bsp19I	CCATGG	1 cut(s)	368
BspACI	CCGC	9 cut(s)	20, 23, 62, 65, 140, 203, 241, 1127, 1155
BspANI	GGCC	1 cut(s)	882
BspCNI	CTCAG	3 cut(s)	90, 247, 1098
BspFNI	CGCG	1 cut(s)	356
BspLI	GGNNCC	3 cut(s)	87, 389, 653
BspMI	ACCTGC	1 cut(s)	724
BspOI	GCTAGC	1 cut(s)	731
BspPI	GGATC	2 cut(s)	486, 635
BsrI	ACTGG	3 cut(s)	544, 922, 1030
BssECI	CCNNGG	5 cut(s)	368, 456, 613, 722, 843
BssMI	GATC	6 cut(s)	48, 394, 478, 627, 886, 1075
BssNI	GRCGYC	1 cut(s)	170
BssT1I	CCWWGG	1 cut(s)	368
Bst2UI	CCWGG	5 cut(s)	458, 515, 615, 723, 845
Bst4CI	ACNGT	2 cut(s)	584, 601
Bst6I	CTCTTC	1 cut(s)	75
BstACI	GRCGYC	1 cut(s)	170
BstAPI	GCANNNNNTGC	1 cut(s)	341
BstBAI	YACGTR	2 cut(s)	436, 1136
BstC8I	GCNNGC	2 cut(s)	729, 856
BstDEI	CTNAG	4 cut(s)	98, 255, 1009, 1085
BstDSI	CCRYGG	1 cut(s)	368
BstENI	CCTNNNNNAGG	1 cut(s)	720
BstF5I	GGATG	5 cut(s)	187, 249, 319, 337, 980
BstFNI	CGCG	1 cut(s)	356
BstHHI	GCGC	1 cut(s)	706
BstKTI	GATC	6 cut(s)	51, 397, 481, 630, 889, 1078
BstMBI	GATC	6 cut(s)	48, 394, 478, 627, 886, 1075
BstMWI	GCNNNNNNNGC	3 cut(s)	314, 341, 703
BstNI	CCWGG	5 cut(s)	458, 515, 615, 723, 845
BstNSI	RCATGY	1 cut(s)	148
BstSCI	CCNGG	5 cut(s)	456, 513, 613, 721, 843
BstSLI	GKGCMC	1 cut(s)	1135
BstUI	CGCG	1 cut(s)	356
BstV1I	GCAGC	5 cut(s)	174, 304, 347, 386, 1013
BstXI	CCANNNNNNTGG	1 cut(s)	962
BsuI	GTATCC	1 cut(s)	944
BsuRI	GGCC	1 cut(s)	882
BtgI	CCRYGG	1 cut(s)	368
BtgZI	GCGATG	1 cut(s)	873
BtsCI	GGATG	5 cut(s)	187, 249, 319, 337, 980
BtsIMutI	CAGTG	2 cut(s)	589, 1023
BveI	ACCTGC	1 cut(s)	724
Cac8I	GCNNGC	2 cut(s)	729, 856
CfoI	GCGC	1 cut(s)	706
Cfr13I	GGNCC	3 cut(s)	406, 1113, 1157
CseI	GACGC	2 cut(s)	178, 345
Csp6I	GTAC	2 cut(s)	537, 1089
CviAII	CATG	8 cut(s)	77, 145, 369, 392, 688, 884, 956, 1059
CviQI	GTAC	2 cut(s)	537, 1089
DdeI	CTNAG	4 cut(s)	98, 255, 1009, 1085
DpnI	GATC	6 cut(s)	50, 396, 480, 629, 888, 1077
DpnII	GATC	6 cut(s)	48, 394, 478, 627, 886, 1075
Eam1104I	CTCTTC	1 cut(s)	75
EarI	CTCTTC	1 cut(s)	75
EciI	GGCGGA	1 cut(s)	155
Eco130I	CCWWGG	1 cut(s)	368
Eco47I	GGWCC	3 cut(s)	406, 1113, 1157
Eco57I	CTGAAG	1 cut(s)	648
EcoNI	CCTNNNNNAGG	1 cut(s)	720
EcoRII	CCWGG	5 cut(s)	456, 513, 613, 721, 843
EcoT14I	CCWWGG	1 cut(s)	368
EcoT22I	ATGCAT	1 cut(s)	961
ErhI	CCWWGG	1 cut(s)	368
FaeI	CATG	8 cut(s)	80, 148, 372, 395, 691, 887, 959, 1062
FatI	CATG	8 cut(s)	76, 144, 368, 391, 687, 883, 955, 1058
FauI	CCCGC	2 cut(s)	196, 1120
FauNDI	CATATG	1 cut(s)	961
FbaI	TGATCA	2 cut(s)	394, 886
Fnu4HI	GCNGC	9 cut(s)	21, 24, 63, 66, 188, 318, 336, 375, 1002
FokI	GGATG	5 cut(s)	194, 256, 326, 344, 987
Fsp4HI	GCNGC	9 cut(s)	21, 24, 63, 66, 188, 318, 336, 375, 1002
FspBI	CTAG	2 cut(s)	728, 762
GlaI	GCGC	1 cut(s)	705
GluI	GCNGC	9 cut(s)	21, 24, 63, 66, 188, 318, 336, 375, 1002
GsaI	CCCAGC	3 cut(s)	350, 643, 942
GsuI	CTGGAG	3 cut(s)	536, 705, 1047
HaeIII	GGCC	1 cut(s)	882
HapII	CCGG	1 cut(s)	282
HgaI	GACGC	2 cut(s)	178, 345
HhaI	GCGC	1 cut(s)	706
Hin1I	GRCGYC	1 cut(s)	170
Hin1II	CATG	8 cut(s)	80, 148, 372, 395, 691, 887, 959, 1062
Hin6I	GCGC	1 cut(s)	704
HinP1I	GCGC	1 cut(s)	704
HinfI	GANTC	4 cut(s)	247, 401, 770, 945
HpaII	CCGG	1 cut(s)	282
HphI	GGTGA	2 cut(s)	271, 881
Hpy166II	GTNNAC	2 cut(s)	823, 1133
Hpy188I	TCNGA	5 cut(s)	48, 214, 406, 478, 950
Hpy188III	TCNNGA	4 cut(s)	398, 482, 502, 1111
Hpy8I	GTNNAC	2 cut(s)	823, 1133
Hpy99I	CGWCG	3 cut(s)	172, 229, 1076
HpyAV	CCTTC	4 cut(s)	413, 483, 794, 961
HpyCH4III	ACNGT	2 cut(s)	584, 601
HpyCH4IV	ACGT	2 cut(s)	435, 1135
HpyCH4V	TGCA	5 cut(s)	133, 148, 335, 959, 1133
HpyF10VI	GCNNNNNNNGC	3 cut(s)	314, 341, 703
HpyF3I	CTNAG	4 cut(s)	98, 255, 1009, 1085
HpySE526I	ACGT	2 cut(s)	435, 1135
Hsp92I	GRCGYC	1 cut(s)	170
Hsp92II	CATG	8 cut(s)	80, 148, 372, 395, 691, 887, 959, 1062
HspAI	GCGC	1 cut(s)	704
Ksp22I	TGATCA	2 cut(s)	394, 886
Kzo9I	GATC	6 cut(s)	48, 394, 478, 627, 886, 1075
LmnI	GCTCC	2 cut(s)	724, 1037
Lsp1109I	GCAGC	5 cut(s)	174, 304, 347, 386, 1013
LweI	GCATC	3 cut(s)	295, 304, 322
MaeI	CTAG	2 cut(s)	728, 762
MaeII	ACGT	2 cut(s)	435, 1135
MaeIII	GTNAC	1 cut(s)	192
MalI	GATC	6 cut(s)	50, 396, 480, 629, 888, 1077
MboI	GATC	6 cut(s)	48, 394, 478, 627, 886, 1075
MboII	GAAGA	2 cut(s)	92, 826
MhlI	GDGCHC	1 cut(s)	1135
MluCI	AATT	2 cut(s)	265, 644
MlyI	GAGTC	2 cut(s)	410, 764
MmeI	TCCRAC	4 cut(s)	384, 384, 605, 679
Mph1103I	ATGCAT	1 cut(s)	961
MseI	TTAA	3 cut(s)	123, 219, 441
MslI	CAYNNNNRTG	3 cut(s)	788, 894, 960
MspA1I	CMGCKG	2 cut(s)	241, 377
MspI	CCGG	1 cut(s)	282
MspR9I	CCNGG	5 cut(s)	458, 515, 615, 723, 845
MvaI	CCWGG	5 cut(s)	458, 515, 615, 723, 845
MvnI	CGCG	1 cut(s)	356
MwoI	GCNNNNNNNGC	3 cut(s)	314, 341, 703
NcoI	CCATGG	1 cut(s)	368
NdeI	CATATG	1 cut(s)	961
NdeII	GATC	6 cut(s)	48, 394, 478, 627, 886, 1075
NheI	GCTAGC	1 cut(s)	727
NlaIII	CATG	8 cut(s)	80, 148, 372, 395, 691, 887, 959, 1062
NlaIV	GGNNCC	3 cut(s)	87, 389, 653
NmuCI	GTSAC	1 cut(s)	192
NsiI	ATGCAT	1 cut(s)	961
NspI	RCATGY	1 cut(s)	148
OliI	CACNNNNGTG	1 cut(s)	788
PaqCI	CACCTGC	1 cut(s)	724
PcsI	WCGNNNNNNNCGW	2 cut(s)	23, 410
PfeI	GAWTC	2 cut(s)	247, 945
PkrI	GCNGC	9 cut(s)	22, 25, 64, 67, 189, 319, 337, 376, 1003
PleI	GAGTC	2 cut(s)	409, 764
PpsI	GAGTC	2 cut(s)	409, 764
Ppu21I	YACGTR	2 cut(s)	436, 1136
Psp6I	CCWGG	5 cut(s)	456, 513, 613, 721, 843
PspFI	CCCAGC	3 cut(s)	346, 639, 938
PspGI	CCWGG	5 cut(s)	456, 513, 613, 721, 843
PspN4I	GGNNCC	3 cut(s)	87, 389, 653
PspPI	GGNCC	3 cut(s)	406, 1113, 1157
PsrI	GAACNNNNNNTAC	2 cut(s)	513, 545
PvuII	CAGCTG	1 cut(s)	377
RsaI	GTAC	2 cut(s)	538, 1090
RsaNI	GTAC	2 cut(s)	537, 1089
RseI	CAYNNNNRTG	3 cut(s)	788, 894, 960
SaqAI	TTAA	3 cut(s)	123, 219, 441
SatI	GCNGC	9 cut(s)	21, 24, 63, 66, 188, 318, 336, 375, 1002
Sau3AI	GATC	6 cut(s)	48, 394, 478, 627, 886, 1075
Sau96I	GGNCC	3 cut(s)	406, 1113, 1157
ScaI	AGTACT	1 cut(s)	1090
SchI	GAGTC	2 cut(s)	410, 764
ScrFI	CCNGG	5 cut(s)	458, 515, 615, 723, 845
SduI	GDGCHC	1 cut(s)	1135
SfaNI	GCATC	3 cut(s)	295, 304, 322
SinI	GGWCC	3 cut(s)	406, 1113, 1157
SmiMI	CAYNNNNRTG	3 cut(s)	788, 894, 960
Sse9I	AATT	2 cut(s)	265, 644
SsiI	CCGC	9 cut(s)	20, 23, 62, 65, 140, 203, 241, 1127, 1155
SspMI	CTAG	2 cut(s)	728, 762
StyD4I	CCNGG	5 cut(s)	456, 513, 613, 721, 843
StyI	CCWWGG	1 cut(s)	368
TaaI	ACNGT	2 cut(s)	584, 601
TaiI	ACGT	2 cut(s)	438, 1138
TaqI	TCGA	2 cut(s)	413, 1071
TasI	AATT	2 cut(s)	265, 644
TatI	WGTACW	1 cut(s)	1088
TauI	GCSGC	4 cut(s)	23, 26, 65, 68
TfiI	GAWTC	2 cut(s)	247, 945
Tru1I	TTAA	3 cut(s)	123, 219, 441
Tru9I	TTAA	3 cut(s)	123, 219, 441
TscAI	CASTG	2 cut(s)	589, 1030
TseFI	GTSAC	1 cut(s)	192
TseI	GCWGC	5 cut(s)	187, 317, 335, 374, 1001
Tsp45I	GTSAC	1 cut(s)	192
TspDTI	ATGAA	4 cut(s)	93, 259, 443, 704
TspGWI	ACGGA	2 cut(s)	326, 808
TspRI	CASTG	2 cut(s)	589, 1030
VneI	GTGCAC	1 cut(s)	1131
VpaK11BI	GGWCC	3 cut(s)	406, 1113, 1157
XagI	CCTNNNNNAGG	1 cut(s)	720
XceI	RCATGY	1 cut(s)	148
XspI	CTAG	2 cut(s)	728, 762
ZrmI	AGTACT	1 cut(s)	1090
Zsp2I	ATGCAT	1 cut(s)	961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.