AT5G53600

X8 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000486
Physical Location & Seq
Forward (+)
21777107 .. 21777523
417 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53600.1

Sequence Viewer

Length: 336 bp
ATGTCTTTAAAACTTTTGACGTTCCTCTTTCTCCTATCTGCTGCTGTGATCTACCACATTCCCGTCGTGACTTGCAGAAGAACATGGTGCACGGCGATGCCTACTTCCACAACTGAGCAGTTACAATCCAACATTAACTTTGCTTGCAATCATGTGGATTGTGCACCGATCCAACCCGGTGGATTTTGCTATTACCCTAATACTTTACTAGACCATGCTGCGTTTGCCATGACCAGATACTACAGGAGTCAAGGTCACACATATGCTGCTTGTAGCTTCGGCAATACTGGTTACATTATATCTTCCGACCCAAGTGTTGGCACATGTATCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

111

Amino Acids

12.29

Weight (kDa)

7.6

Isoelectric Point (pI)

60.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 28 - 97 5.4e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29735 AT4G09090 AT4G09462 AT4G09464 AT4G09465 AT4G09466 AT4G09467 AT5G53600 AT5G53610 AT5G63225 AT5G63230
fragaria_vesca FvH4_3g06020 FvH4_3g06020 FvH4_3g06020 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06040 FvH4_4g08590 FvH4_6g37860
malus_domestica MD10G1265100.v1.1
prunus_persica Prupe.4G076700_v2.0.a1 Prupe.4G076900_v2.0.a1 Prupe.4G084600_v2.0.a1 Prupe.4G130100_v2.0.a1 Prupe.6G294200_v2.0.a1
pyrus_communis pycom05g26640 pycom10g22110
rosa_chinensis RchiOBHm_Chr5g0013281 RchiOBHm_Chr5g0013291 RchiOBHm_Chr5g0013321 RchiOBHm_Chr5g0013341 RchiOBHm_Chr5g0013351 RchiOBHm_Chr5g0013361 RchiOBHm_Chr5g0013371 RchiOBHm_Chr5g0013381 RchiOBHm_Chr5g0013391
rosa_laevigata RLG00000032004 RLG00000032005 RLG00000032008 RLG00000032012 RLG00000032013 RLG00000032014 RLG00000032015 RLG00000032016
rosa_multiflora Rmu_co8200260.1_g000001 Rmu_co8451143.1_g000001 Rmu_sc0003458.1_g000006 Rmu_sc0003458.1_g000008 Rmu_sc0003458.1_g000009 Rmu_sc0003458.1_g000012 Rmu_sc0003458.1_g000014 Rmu_sc0010713.1_g000001
rosa_roxburghii Rroxscaffold_1G00063160 Rroxscaffold_1G00063170 Rroxscaffold_1G00063180 Rroxscaffold_1G00063190 Rroxscaffold_1G00063200 Rroxscaffold_1G00063210
rosa_rugosa Rorug05G0008700 Rorug05G0008700 Rorug05G0008800 Rorug05G0008800 Rorug05G0008900.1 Rorug05G0009000 Rorug05G0009000 Rorug05G0009000
rosa_samantha Rh5AG103700 Rh5AG103800 Rh5AG104000 Rh5AG104300 Rh5AG104400 Rh5BG100300 Rh5BG100400 Rh5BG100500 Rh5BG100600 Rh5BG100700 Rh5CG111500 Rh5CG111600 Rh5CG112000 Rh5CG112200 Rh5CG112300 Rh5CG112500 Rh5DG099200 Rh5DG099300
rosa_wichuraiana Rw5G009040 Rw5G009050 Rw5G009080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 317
AclWI GGATC 1 cut(s) 163
AfiI CCNNNNNNNGG 1 cut(s) 317
AflIII ACRYGT 1 cut(s) 323
AluBI AGCT 1 cut(s) 276
AluI AGCT 1 cut(s) 276
Alw21I GWGCWC 2 cut(s) 92, 166
Alw44I GTGCAC 2 cut(s) 88, 162
AlwI GGATC 1 cut(s) 163
ApaLI GTGCAC 2 cut(s) 88, 162
ApeKI GCWGC 3 cut(s) 41, 218, 266
AsuC2I CCSGG 1 cut(s) 177
BaeGI GKGCMC 2 cut(s) 92, 166
Bbv12I GWGCWC 2 cut(s) 92, 166
BbvI GCAGC 3 cut(s) 28, 205, 253
BceAI ACGGC 1 cut(s) 108
BcnI CCSGG 1 cut(s) 177
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 241
BisI GCNGC 3 cut(s) 42, 219, 267
BlsI GCNGC 3 cut(s) 43, 220, 268
Bme1390I CCNGG 1 cut(s) 177
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 87
BpuMI CCSGG 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse1I ACTGG 1 cut(s) 292
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 1 cut(s) 292
BseSI GKGCMC 2 cut(s) 92, 166
BseXI GCAGC 3 cut(s) 28, 205, 253
BsiHKAI GWGCWC 2 cut(s) 92, 166
BsiSI CCGG 1 cut(s) 177
BslI CCNNNNNNNGG 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 92, 166
Bsp143I GATC 2 cut(s) 48, 168
BspCNI CTCAG 1 cut(s) 106
BspPI GGATC 1 cut(s) 163
BsrI ACTGG 1 cut(s) 292
BssMI GATC 2 cut(s) 48, 168
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 1 cut(s) 114
BstKTI GATC 2 cut(s) 51, 171
BstMBI GATC 2 cut(s) 48, 168
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNSI RCATGY 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 175
BstSFI CTRYAG 1 cut(s) 241
BstSLI GKGCMC 2 cut(s) 92, 166
BstV1I GCAGC 3 cut(s) 28, 205, 253
BstXI CCANNNNNNTGG 1 cut(s) 179
BtgZI GCGATG 1 cut(s) 110
Cac8I GCNNGC 1 cut(s) 145
CviAII CATG 5 cut(s) 84, 152, 215, 229, 324
CviJI RGCY 1 cut(s) 276
CviKI_1 RGCY 1 cut(s) 276
DdeI CTNAG 1 cut(s) 114
DpnI GATC 2 cut(s) 50, 170
DpnII GATC 2 cut(s) 48, 168
DraI TTTAAA 1 cut(s) 9
FaeI CATG 5 cut(s) 87, 155, 218, 232, 327
FaiI YATR 8 cut(s) 85, 153, 216, 230, 262, 264, 299, 325
FatI CATG 5 cut(s) 83, 151, 214, 228, 323
FauNDI CATATG 1 cut(s) 262
Fnu4HI GCNGC 3 cut(s) 42, 219, 267
Fsp4HI GCNGC 3 cut(s) 42, 219, 267
FspBI CTAG 1 cut(s) 209
GluI GCNGC 3 cut(s) 42, 219, 267
HapII CCGG 1 cut(s) 177
Hin1II CATG 5 cut(s) 87, 155, 218, 232, 327
HinfI GANTC 1 cut(s) 247
HpaII CCGG 1 cut(s) 177
Hpy166II GTNNAC 2 cut(s) 90, 164
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 1 cut(s) 67
Hpy8I GTNNAC 2 cut(s) 90, 164
Hpy99I CGWCG 1 cut(s) 68
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 4 cut(s) 75, 90, 147, 164
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 1 cut(s) 20
Hsp92II CATG 5 cut(s) 87, 155, 218, 232, 327
Kzo9I GATC 2 cut(s) 48, 168
LpnPI CCDG 4 cut(s) 190, 229, 247, 273
Lsp1109I GCAGC 3 cut(s) 28, 205, 253
LweI GCATC 1 cut(s) 87
MaeI CTAG 1 cut(s) 209
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 4 cut(s) 67, 120, 254, 290
MalI GATC 2 cut(s) 50, 170
MboI GATC 2 cut(s) 48, 168
MboII GAAGA 2 cut(s) 90, 294
MhlI GDGCHC 2 cut(s) 92, 166
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 3 cut(s) 153, 196, 330
MnlI CCTC 1 cut(s) 35
MseI TTAA 3 cut(s) 8, 135, 334
MslI CAYNNNNRTG 2 cut(s) 95, 261
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 1 cut(s) 177
MwoI GCNNNNNNNGC 1 cut(s) 224
NciI CCSGG 1 cut(s) 177
NdeI CATATG 1 cut(s) 262
NdeII GATC 2 cut(s) 48, 168
NlaIII CATG 5 cut(s) 87, 155, 218, 232, 327
NmuCI GTSAC 2 cut(s) 67, 254
NspI RCATGY 1 cut(s) 327
PciI ACATGT 1 cut(s) 323
PflMI CCANNNNNTGG 1 cut(s) 317
PkrI GCNGC 3 cut(s) 43, 220, 268
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
PscI ACATGT 1 cut(s) 323
RseI CAYNNNNRTG 2 cut(s) 95, 261
SaqAI TTAA 3 cut(s) 8, 135, 334
SatI GCNGC 3 cut(s) 42, 219, 267
Sau3AI GATC 2 cut(s) 48, 168
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 2 cut(s) 92, 166
SetI ASST 3 cut(s) 23, 256, 278
SfaNI GCATC 1 cut(s) 87
SfcI CTRYAG 1 cut(s) 241
SmiMI CAYNNNNRTG 2 cut(s) 95, 261
SspMI CTAG 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 175
TaiI ACGT 1 cut(s) 23
Tru1I TTAA 3 cut(s) 8, 135, 334
Tru9I TTAA 3 cut(s) 8, 135, 334
TseFI GTSAC 2 cut(s) 67, 254
TseI GCWGC 3 cut(s) 41, 218, 266
Tsp45I GTSAC 2 cut(s) 67, 254
Van91I CCANNNNNTGG 1 cut(s) 317
VneI GTGCAC 2 cut(s) 88, 162
XceI RCATGY 1 cut(s) 327
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.