AT5G53610

X8 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000486
Physical Location & Seq
Forward (+)
21779023 .. 21779499
477 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53610.1

Sequence Viewer

Length: 333 bp
ATGTCTTTACAATTTTTGACGCTCATCTTTCTCCTTTCTGCTGCTGTGATCTATCACATCCCCGTCGTGACTTGCGAACCATGGTGCTCGGCGATGCCTTCTTCCACACCTGAGCAGTTACAAGCCAACATTCAGCTTGCTTGCAGTCGTGTGGATTGTACACCGATCCAACCCGGTGGATTTTGCTATTACCCTAATACTTTACTAGACCATGCTTCGTTTGTCATGAACTCTTACTACAAGAGTCAAGGTCGCACTTATGCTGCTTGTAGCTTCGGCAATACTGGTTACCTTATTTATTCCGACCCAAGTACTGGCACATGTGAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

110

Amino Acids

12.13

Weight (kDa)

4.92

Isoelectric Point (pI)

59.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 28 - 96 2.9e-22 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29735 AT4G09090 AT4G09462 AT4G09464 AT4G09465 AT4G09466 AT4G09467 AT5G53600 AT5G53610 AT5G63225 AT5G63230
fragaria_vesca FvH4_3g06020 FvH4_3g06020 FvH4_3g06020 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06040 FvH4_4g08590 FvH4_6g37860
malus_domestica MD10G1265100.v1.1
prunus_persica Prupe.4G076700_v2.0.a1 Prupe.4G076900_v2.0.a1 Prupe.4G084600_v2.0.a1 Prupe.4G130100_v2.0.a1 Prupe.6G294200_v2.0.a1
pyrus_communis pycom05g26640 pycom10g22110
rosa_chinensis RchiOBHm_Chr5g0013281 RchiOBHm_Chr5g0013291 RchiOBHm_Chr5g0013321 RchiOBHm_Chr5g0013341 RchiOBHm_Chr5g0013351 RchiOBHm_Chr5g0013361 RchiOBHm_Chr5g0013371 RchiOBHm_Chr5g0013381 RchiOBHm_Chr5g0013391
rosa_laevigata RLG00000032004 RLG00000032005 RLG00000032008 RLG00000032012 RLG00000032013 RLG00000032014 RLG00000032015 RLG00000032016
rosa_multiflora Rmu_co8200260.1_g000001 Rmu_co8451143.1_g000001 Rmu_sc0003458.1_g000006 Rmu_sc0003458.1_g000008 Rmu_sc0003458.1_g000009 Rmu_sc0003458.1_g000012 Rmu_sc0003458.1_g000014 Rmu_sc0010713.1_g000001
rosa_roxburghii Rroxscaffold_1G00063160 Rroxscaffold_1G00063170 Rroxscaffold_1G00063180 Rroxscaffold_1G00063190 Rroxscaffold_1G00063200 Rroxscaffold_1G00063210
rosa_rugosa Rorug05G0008700 Rorug05G0008700 Rorug05G0008800 Rorug05G0008800 Rorug05G0008900.1 Rorug05G0009000 Rorug05G0009000 Rorug05G0009000
rosa_samantha Rh5AG103700 Rh5AG103800 Rh5AG104000 Rh5AG104300 Rh5AG104400 Rh5BG100300 Rh5BG100400 Rh5BG100500 Rh5BG100600 Rh5BG100700 Rh5CG111500 Rh5CG111600 Rh5CG112000 Rh5CG112200 Rh5CG112300 Rh5CG112500 Rh5DG099200 Rh5DG099300
rosa_wichuraiana Rw5G009040 Rw5G009050 Rw5G009080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 314
AclWI GGATC 1 cut(s) 160
AcsI RAATTY 1 cut(s) 326
AfaI GTAC 2 cut(s) 160, 313
AfiI CCNNNNNNNGG 1 cut(s) 314
AflIII ACRYGT 1 cut(s) 320
AluBI AGCT 2 cut(s) 136, 273
AluI AGCT 2 cut(s) 136, 273
Alw21I GWGCWC 1 cut(s) 89
AlwI GGATC 1 cut(s) 160
ApeKI GCWGC 2 cut(s) 41, 263
ApoI RAATTY 1 cut(s) 326
AsuC2I CCSGG 1 cut(s) 174
Bbv12I GWGCWC 1 cut(s) 89
BbvI GCAGC 2 cut(s) 28, 250
BcnI CCSGG 1 cut(s) 174
BfaI CTAG 1 cut(s) 206
BisI GCNGC 2 cut(s) 42, 264
BlsI GCNGC 2 cut(s) 43, 265
BmcAI AGTACT 1 cut(s) 313
Bme1390I CCNGG 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 84
Bpu10I CCTNAGC 1 cut(s) 111
BpuMI CCSGG 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 80
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse1I ACTGG 2 cut(s) 289, 319
BseDI CCNNGG 1 cut(s) 80
BseGI GGATG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 2 cut(s) 289, 319
BseXI GCAGC 2 cut(s) 28, 250
BsiHKAI GWGCWC 1 cut(s) 89
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 89
Bsp1407I TGTACA 1 cut(s) 158
Bsp143I GATC 2 cut(s) 48, 165
Bsp19I CCATGG 1 cut(s) 80
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 1 cut(s) 225
BspPI GGATC 1 cut(s) 160
BsrGI TGTACA 1 cut(s) 158
BsrI ACTGG 2 cut(s) 289, 319
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 2 cut(s) 48, 165
BssT1I CCWWGG 1 cut(s) 80
BstAUI TGTACA 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 138, 142
BstDEI CTNAG 1 cut(s) 111
BstDSI CCRYGG 1 cut(s) 80
BstEII GGTNACC 1 cut(s) 287
BstF5I GGATG 1 cut(s) 57
BstKTI GATC 2 cut(s) 51, 168
BstMBI GATC 2 cut(s) 48, 165
BstNSI RCATGY 1 cut(s) 324
BstPI GGTNACC 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 172
BstV1I GCAGC 2 cut(s) 28, 250
BstXI CCANNNNNNTGG 1 cut(s) 176
BtgI CCRYGG 1 cut(s) 80
BtgZI GCGATG 1 cut(s) 107
BtsCI GGATG 1 cut(s) 57
Cac8I GCNNGC 2 cut(s) 138, 142
CciI TCATGA 1 cut(s) 225
CseI GACGC 1 cut(s) 28
Csp6I GTAC 2 cut(s) 159, 312
CviAII CATG 4 cut(s) 81, 212, 226, 321
CviJI RGCY 3 cut(s) 125, 136, 273
CviKI_1 RGCY 3 cut(s) 125, 136, 273
CviQI GTAC 2 cut(s) 159, 312
DdeI CTNAG 1 cut(s) 111
DpnI GATC 2 cut(s) 50, 167
DpnII GATC 2 cut(s) 48, 165
Eco130I CCWWGG 1 cut(s) 80
Eco91I GGTNACC 1 cut(s) 287
EcoO65I GGTNACC 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 80
ErhI CCWWGG 1 cut(s) 80
FaeI CATG 4 cut(s) 84, 215, 229, 324
FaiI YATR 5 cut(s) 82, 213, 227, 261, 322
FatI CATG 4 cut(s) 80, 211, 225, 320
Fnu4HI GCNGC 2 cut(s) 42, 264
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 2 cut(s) 42, 264
FspBI CTAG 1 cut(s) 206
GluI GCNGC 2 cut(s) 42, 264
HapII CCGG 1 cut(s) 174
HgaI GACGC 1 cut(s) 28
Hin1II CATG 4 cut(s) 84, 215, 229, 324
HinfI GANTC 1 cut(s) 244
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 1 cut(s) 304
Hpy188III TCNNGA 2 cut(s) 67, 226
Hpy8I GTNNAC 1 cut(s) 161
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 1 cut(s) 108
HpyCH4V TGCA 1 cut(s) 144
HpyF3I CTNAG 1 cut(s) 111
Hsp92II CATG 4 cut(s) 84, 215, 229, 324
Kzo9I GATC 2 cut(s) 48, 165
LpnPI CCDG 4 cut(s) 123, 187, 270, 300
Lsp1109I GCAGC 2 cut(s) 28, 250
LweI GCATC 1 cut(s) 84
MaeI CTAG 1 cut(s) 206
MaeIII GTNAC 3 cut(s) 67, 117, 287
MalI GATC 2 cut(s) 50, 167
MboI GATC 2 cut(s) 48, 165
MboII GAAGA 1 cut(s) 93
MhlI GDGCHC 1 cut(s) 89
MluCI AATT 2 cut(s) 11, 326
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 2 cut(s) 193, 327
MseI TTAA 1 cut(s) 331
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 174
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 1 cut(s) 80
NdeII GATC 2 cut(s) 48, 165
NlaIII CATG 4 cut(s) 84, 215, 229, 324
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 67
NspI RCATGY 1 cut(s) 324
PagI TCATGA 1 cut(s) 225
PciI ACATGT 1 cut(s) 320
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PflMI CCANNNNNTGG 1 cut(s) 314
PkrI GCNGC 2 cut(s) 43, 265
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
PscI ACATGT 1 cut(s) 320
PspEI GGTNACC 1 cut(s) 287
PsrI GAACNNNNNNTAC 2 cut(s) 221, 253
RsaI GTAC 2 cut(s) 160, 313
RsaNI GTAC 2 cut(s) 159, 312
SaqAI TTAA 1 cut(s) 331
SatI GCNGC 2 cut(s) 42, 264
Sau3AI GATC 2 cut(s) 48, 165
ScaI AGTACT 1 cut(s) 313
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 1 cut(s) 89
SetI ASST 5 cut(s) 112, 138, 253, 275, 294
SfaNI GCATC 1 cut(s) 84
Sse9I AATT 2 cut(s) 11, 326
SspMI CTAG 1 cut(s) 206
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 1 cut(s) 80
TasI AATT 2 cut(s) 11, 326
TatI WGTACW 2 cut(s) 158, 311
Tru1I TTAA 1 cut(s) 331
Tru9I TTAA 1 cut(s) 331
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 2 cut(s) 41, 263
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 1 cut(s) 242
Van91I CCANNNNNTGG 1 cut(s) 314
XapI RAATTY 1 cut(s) 326
XceI RCATGY 1 cut(s) 324
XspI CTAG 1 cut(s) 206
ZrmI AGTACT 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.