Rh5DG099300

X8 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000486
Physical Location & Seq
Forward (+)
9117206 .. 9125107
7902 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG099300.1

Sequence Viewer

Length: 561 bp
ATGGCGAATGTTCTTGTCCTTCGCACTGTTGTTATCGTGGCTTTCCTCGCTCTCTCGAACCTCGAGCTCTGCGCCATTGCTGATCTTCCAGGACATGGCCATGCCGCTTTCATGGCAGGCATTGCTAAGAATACCTGGTGTGTGGCAAAACCAACTGCCGCTGATAGTTTGCTACAATTGAACATTGACTTTGCTTGTAGTAAAGTCAACTGTAGTGTAATTGAACCCGGTGGTGAATACCAATTACCAGACACTCTAATGAACCACGCGTCTGTTGCCATGAATCTTTACTACCAATCTTTTGGCAGAACAGATTTGAGCTGTTATTTTCAGTCGACCGGCATGGTTGTGATTGAAGATCCAAGTTCTGGAACCCTTGTGTCTATGAAGGGGTTGTCCTTCTCACAAATCCGACAACCCAAGTCCGATCCTCTCAGAAAACATAGTGGGGGAGAACTGTGTTCGTTTGGTGGTGTTCTTCCGCGTCATACTGTAATTGTATTCACTCGGAGAAACTCTTTGGTTGTTGAAGAAATCTTATTTGGTGATTTTTACGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

186

Amino Acids

20.12

Weight (kDa)

6.17

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 45 - 115 3.4e-17 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29735 AT4G09090 AT4G09462 AT4G09464 AT4G09465 AT4G09466 AT4G09467 AT5G53600 AT5G53610 AT5G63225 AT5G63230
fragaria_vesca FvH4_3g06020 FvH4_3g06020 FvH4_3g06020 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06040 FvH4_4g08590 FvH4_6g37860
malus_domestica MD10G1265100.v1.1
prunus_persica Prupe.4G076700_v2.0.a1 Prupe.4G076900_v2.0.a1 Prupe.4G084600_v2.0.a1 Prupe.4G130100_v2.0.a1 Prupe.6G294200_v2.0.a1
pyrus_communis pycom05g26640 pycom10g22110
rosa_chinensis RchiOBHm_Chr5g0013281 RchiOBHm_Chr5g0013291 RchiOBHm_Chr5g0013321 RchiOBHm_Chr5g0013341 RchiOBHm_Chr5g0013351 RchiOBHm_Chr5g0013361 RchiOBHm_Chr5g0013371 RchiOBHm_Chr5g0013381 RchiOBHm_Chr5g0013391
rosa_laevigata RLG00000032004 RLG00000032005 RLG00000032008 RLG00000032012 RLG00000032013 RLG00000032014 RLG00000032015 RLG00000032016
rosa_multiflora Rmu_co8200260.1_g000001 Rmu_co8451143.1_g000001 Rmu_sc0003458.1_g000006 Rmu_sc0003458.1_g000008 Rmu_sc0003458.1_g000009 Rmu_sc0003458.1_g000012 Rmu_sc0003458.1_g000014 Rmu_sc0010713.1_g000001
rosa_roxburghii Rroxscaffold_1G00063160 Rroxscaffold_1G00063170 Rroxscaffold_1G00063180 Rroxscaffold_1G00063190 Rroxscaffold_1G00063200 Rroxscaffold_1G00063210
rosa_rugosa Rorug05G0008700 Rorug05G0008700 Rorug05G0008800 Rorug05G0008800 Rorug05G0008900.1 Rorug05G0009000 Rorug05G0009000 Rorug05G0009000
rosa_samantha Rh5AG103700 Rh5AG103800 Rh5AG104000 Rh5AG104300 Rh5AG104400 Rh5BG100300 Rh5BG100400 Rh5BG100500 Rh5BG100600 Rh5BG100700 Rh5CG111500 Rh5CG111600 Rh5CG112000 Rh5CG112200 Rh5CG112300 Rh5CG112500 Rh5DG099200 Rh5DG099300
rosa_wichuraiana Rw5G009040 Rw5G009050 Rw5G009080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 95, 368
AccI GTMKAC 1 cut(s) 335
AccII CGCG 2 cut(s) 269, 484
AciI CCGC 3 cut(s) 105, 159, 482
AclWI GGATC 2 cut(s) 353, 422
AcoI YGGCCR 1 cut(s) 97
AfiI CCNNNNNNNGG 2 cut(s) 95, 368
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 4 cut(s) 181, 224, 356, 530
AjnI CCWGG 2 cut(s) 88, 134
AjuI GAANNNNNNNTTGG 2 cut(s) 525, 557
AluBI AGCT 2 cut(s) 67, 321
AluI AGCT 2 cut(s) 67, 321
Alw21I GWGCWC 1 cut(s) 69
AlwI GGATC 2 cut(s) 353, 422
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 1 cut(s) 97
AspLEI GCGC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 2 cut(s) 245, 557
AvaI CYCGRG 1 cut(s) 62
BalI TGGCCA 1 cut(s) 99
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 69
BciT130I CCWGG 2 cut(s) 90, 136
BcnI CCSGG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 211
BisI GCNGC 2 cut(s) 105, 159
BlsI GCNGC 2 cut(s) 106, 160
Bme1390I CCNGG 3 cut(s) 90, 136, 228
BmeT110I CYCGRG 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 3 cut(s) 90, 136, 228
BpuMI CCSGG 1 cut(s) 228
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 368
Bse118I RCCGGY 1 cut(s) 338
Bse3DI GCAATG 2 cut(s) 75, 120
BseBI CCWGG 2 cut(s) 90, 136
BseLI CCNNNNNNNGG 2 cut(s) 95, 368
BseMI GCAATG 2 cut(s) 75, 120
BseMII CTCAG 1 cut(s) 448
Bsh1236I CGCG 2 cut(s) 269, 484
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 1 cut(s) 99
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 69
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 2 cut(s) 228, 339
BslI CCNNNNNNNGG 2 cut(s) 95, 368
BsnI GGCC 1 cut(s) 99
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 3 cut(s) 82, 358, 427
BspACI CCGC 3 cut(s) 105, 159, 482
BspANI GGCC 1 cut(s) 99
BspCNI CTCAG 1 cut(s) 447
BspFNI CGCG 2 cut(s) 269, 484
BspLI GGNNCC 1 cut(s) 373
BspPI GGATC 2 cut(s) 353, 422
BsrDI GCAATG 2 cut(s) 75, 120
BsrFI RCCGGY 1 cut(s) 338
BssAI RCCGGY 1 cut(s) 338
BssMI GATC 3 cut(s) 82, 358, 427
Bst2UI CCWGG 2 cut(s) 90, 136
Bst4CI ACNGT 4 cut(s) 28, 212, 459, 493
BstAPI GCANNNNNTGC 1 cut(s) 122
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 2 cut(s) 126, 434
BstFNI CGCG 2 cut(s) 269, 484
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 3 cut(s) 85, 361, 430
BstMBI GATC 3 cut(s) 82, 358, 427
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 4 cut(s) 47, 113, 122, 275
BstNI CCWGG 2 cut(s) 90, 136
BstSCI CCNGG 3 cut(s) 88, 134, 226
BstSFI CTRYAG 1 cut(s) 211
BstUI CGCG 2 cut(s) 269, 484
BstX2I RGATCY 1 cut(s) 358
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 1 cut(s) 118
CfoI GCGC 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 338
CseI GACGC 2 cut(s) 258, 473
CsiI ACCWGGT 1 cut(s) 134
CviAII CATG 5 cut(s) 95, 101, 112, 280, 343
CviJI RGCY 4 cut(s) 41, 67, 99, 321
CviKI_1 RGCY 4 cut(s) 41, 67, 99, 321
DdeI CTNAG 2 cut(s) 126, 434
DpnI GATC 3 cut(s) 84, 360, 429
DpnII GATC 3 cut(s) 82, 358, 427
EaeI YGGCCR 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 69
Eco53kI GAGCTC 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 88, 134
EcoT38I GRGCYC 1 cut(s) 69
FaeI CATG 5 cut(s) 98, 104, 115, 283, 346
FaiI YATR 8 cut(s) 96, 102, 113, 281, 344, 386, 444, 489
FatI CATG 5 cut(s) 94, 100, 111, 279, 342
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 2 cut(s) 105, 159
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 2 cut(s) 105, 159
GlaI GCGC 1 cut(s) 73
GluI GCNGC 2 cut(s) 105, 159
HaeIII GGCC 1 cut(s) 99
HapII CCGG 2 cut(s) 228, 339
HgaI GACGC 2 cut(s) 258, 473
HhaI GCGC 1 cut(s) 74
Hin1II CATG 5 cut(s) 98, 104, 115, 283, 346
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HincII GTYRAC 2 cut(s) 208, 336
HindII GTYRAC 2 cut(s) 208, 336
HinfI GANTC 1 cut(s) 283
HpaII CCGG 2 cut(s) 228, 339
HphI GGTGA 2 cut(s) 245, 557
Hpy166II GTNNAC 2 cut(s) 208, 336
Hpy188I TCNGA 4 cut(s) 413, 427, 437, 510
Hpy188III TCNNGA 2 cut(s) 55, 369
Hpy8I GTNNAC 2 cut(s) 208, 336
HpyAV CCTTC 3 cut(s) 29, 382, 409
HpyCH4III ACNGT 4 cut(s) 28, 212, 459, 493
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 113, 122, 275
HpyF3I CTNAG 2 cut(s) 126, 434
Hsp92II CATG 5 cut(s) 98, 104, 115, 283, 346
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 3 cut(s) 82, 358, 427
LpnPI CCDG 9 cut(s) 75, 102, 102, 121, 148, 241, 261, 352, 354
MabI ACCWGGT 1 cut(s) 134
MalI GATC 3 cut(s) 84, 360, 429
MboI GATC 3 cut(s) 82, 358, 427
MboII GAAGA 4 cut(s) 77, 368, 470, 542
MfeI CAATTG 1 cut(s) 176
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 69
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 4 cut(s) 176, 219, 242, 495
MluI ACGCGT 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 436
MnlI CCTC 3 cut(s) 56, 71, 441
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MslI CAYNNNNRTG 3 cut(s) 99, 257, 347
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 228, 339
MspR9I CCNGG 3 cut(s) 90, 136, 228
MunI CAATTG 1 cut(s) 176
MvaI CCWGG 2 cut(s) 90, 136
MvnI CGCG 2 cut(s) 269, 484
MwoI GCNNNNNNNGC 4 cut(s) 47, 113, 122, 275
NciI CCSGG 1 cut(s) 228
NdeII GATC 3 cut(s) 82, 358, 427
NlaIII CATG 5 cut(s) 98, 104, 115, 283, 346
NlaIV GGNNCC 1 cut(s) 373
PaeR7I CTCGAG 1 cut(s) 62
PfeI GAWTC 1 cut(s) 283
PflMI CCANNNNNTGG 2 cut(s) 95, 368
PfoI TCCNGGA 1 cut(s) 88
PkrI GCNGC 2 cut(s) 106, 160
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 88, 134
PspGI CCWGG 2 cut(s) 88, 134
PspN4I GGNNCC 1 cut(s) 373
PspXI VCTCGAGB 1 cut(s) 62
PsuI RGATCY 1 cut(s) 358
RseI CAYNNNNRTG 3 cut(s) 99, 257, 347
SacI GAGCTC 1 cut(s) 69
SalI GTCGAC 1 cut(s) 334
SatI GCNGC 2 cut(s) 105, 159
Sau3AI GATC 3 cut(s) 82, 358, 427
ScrFI CCNGG 3 cut(s) 90, 136, 228
SduI GDGCHC 1 cut(s) 69
SetI ASST 4 cut(s) 63, 69, 137, 323
SexAI ACCWGGT 1 cut(s) 134
SfcI CTRYAG 1 cut(s) 211
Sfr274I CTCGAG 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 62
SmiMI CAYNNNNRTG 3 cut(s) 99, 257, 347
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 4 cut(s) 176, 219, 242, 495
SsiI CCGC 3 cut(s) 105, 159, 482
SstI GAGCTC 1 cut(s) 69
StyD4I CCNGG 3 cut(s) 88, 134, 226
TaaI ACNGT 4 cut(s) 28, 212, 459, 493
TaqI TCGA 3 cut(s) 56, 63, 335
TasI AATT 4 cut(s) 176, 219, 242, 495
TauI GCSGC 2 cut(s) 107, 161
TfiI GAWTC 1 cut(s) 283
TscAI CASTG 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 100, 275, 296, 401
TspRI CASTG 1 cut(s) 31
Van91I CCANNNNNTGG 2 cut(s) 95, 368
XhoI CTCGAG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.