Rroxscaffold_1G00063200

Major pollen allergen Ole e 10-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000486
Physical Location & Seq
Reverse (-)
85149848 .. 85154171
4324 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063200.1

Sequence Viewer

Length: 342 bp
ATGGTCGAATATATCTCCTGGGATGAAGGTTGGGGAGTTTTTGCTGAAGTCAATGAAAACAGCCTTGTAATTAGTCTTCCAGGGGTTTTGAGTGGACTGGTTGTTAGTGGGGATGAGAATAAGACCTGGTGTGTTGCTAAGCCTTCAGCAACTGATTCAGAGCTCTCTGCAAACATTCAGTTTGCCTGCAACCAATTGAAGGATTGCAGGGTGATACAAGAAGGAGGCTCATGCTATAACCCCAACTCTCTCATTAATCATGCCTCGGTGGTGCAGGGCCGGTCCCGAGAATTCAGAGACCTAGTGCGAAAATTTGACAGAAGCCTTATGTTCATTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

113

Amino Acids

12.52

Weight (kDa)

4.86

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 42 - 91 2.4e-11 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29735 AT4G09090 AT4G09462 AT4G09464 AT4G09465 AT4G09466 AT4G09467 AT5G53600 AT5G53610 AT5G63225 AT5G63230
fragaria_vesca FvH4_3g06020 FvH4_3g06020 FvH4_3g06020 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06040 FvH4_4g08590 FvH4_6g37860
malus_domestica MD10G1265100.v1.1
prunus_persica Prupe.4G076700_v2.0.a1 Prupe.4G076900_v2.0.a1 Prupe.4G084600_v2.0.a1 Prupe.4G130100_v2.0.a1 Prupe.6G294200_v2.0.a1
pyrus_communis pycom05g26640 pycom10g22110
rosa_chinensis RchiOBHm_Chr5g0013281 RchiOBHm_Chr5g0013291 RchiOBHm_Chr5g0013321 RchiOBHm_Chr5g0013341 RchiOBHm_Chr5g0013351 RchiOBHm_Chr5g0013361 RchiOBHm_Chr5g0013371 RchiOBHm_Chr5g0013381 RchiOBHm_Chr5g0013391
rosa_laevigata RLG00000032004 RLG00000032005 RLG00000032008 RLG00000032012 RLG00000032013 RLG00000032014 RLG00000032015 RLG00000032016
rosa_multiflora Rmu_co8200260.1_g000001 Rmu_co8451143.1_g000001 Rmu_sc0003458.1_g000006 Rmu_sc0003458.1_g000008 Rmu_sc0003458.1_g000009 Rmu_sc0003458.1_g000012 Rmu_sc0003458.1_g000014 Rmu_sc0010713.1_g000001
rosa_roxburghii Rroxscaffold_1G00063160 Rroxscaffold_1G00063170 Rroxscaffold_1G00063180 Rroxscaffold_1G00063190 Rroxscaffold_1G00063200 Rroxscaffold_1G00063210
rosa_rugosa Rorug05G0008700 Rorug05G0008700 Rorug05G0008800 Rorug05G0008800 Rorug05G0008900.1 Rorug05G0009000 Rorug05G0009000 Rorug05G0009000
rosa_samantha Rh5AG103700 Rh5AG103800 Rh5AG104000 Rh5AG104300 Rh5AG104400 Rh5BG100300 Rh5BG100400 Rh5BG100500 Rh5BG100600 Rh5BG100700 Rh5CG111500 Rh5CG111600 Rh5CG112000 Rh5CG112200 Rh5CG112300 Rh5CG112500 Rh5DG099200 Rh5DG099300
rosa_wichuraiana Rw5G009040 Rw5G009050 Rw5G009080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 290, 311
AcuI CTGAAG 2 cut(s) 66, 129
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 1 cut(s) 199
AjnI CCWGG 3 cut(s) 17, 79, 125
AluBI AGCT 1 cut(s) 163
AluI AGCT 1 cut(s) 163
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 1 cut(s) 291
AlwNI CAGNNNCTG 1 cut(s) 152
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 1 cut(s) 277
ApoI RAATTY 2 cut(s) 290, 311
AseI ATTAAT 1 cut(s) 255
AspS9I GGNCC 2 cut(s) 277, 282
AsuHPI GGTGA 1 cut(s) 223
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 165
BarI GAAGNNNNNNTAC 2 cut(s) 60, 92
BbsI GAAGAC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 165
BciT130I CCWGG 3 cut(s) 19, 81, 127
BcoDI GTCTC 1 cut(s) 291
BfaI CTAG 1 cut(s) 302
BlpI GCTNAGC 1 cut(s) 138
Bme1390I CCNGG 3 cut(s) 19, 81, 127
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 2 cut(s) 277, 282
BmiI GGNNCC 1 cut(s) 284
BmrFI CCNGG 3 cut(s) 19, 81, 127
BpiI GAAGAC 1 cut(s) 68
Bpu1102I GCTNAGC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 3 cut(s) 18, 80, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse118I RCCGGY 1 cut(s) 279
Bse1I ACTGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 333
BseBI CCWGG 3 cut(s) 19, 81, 127
BseDI CCNNGG 3 cut(s) 18, 80, 264
BseGI GGATG 2 cut(s) 28, 118
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMI GCAATG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 102
BsgI GTGCAG 1 cut(s) 293
BshFI GGCC 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 1 cut(s) 280
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 1 cut(s) 291
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 1 cut(s) 279
Bso31I GGTCTC 1 cut(s) 291
BsoBI CYCGRG 1 cut(s) 285
Bsp1286I GDGCHC 1 cut(s) 165
Bsp1720I GCTNAGC 1 cut(s) 138
BspANI GGCC 1 cut(s) 279
BspLI GGNNCC 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 279
BsrI ACTGG 1 cut(s) 102
BssAI RCCGGY 1 cut(s) 279
BssECI CCNNGG 3 cut(s) 18, 80, 264
Bst2UI CCWGG 3 cut(s) 19, 81, 127
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 1 cut(s) 138
BstF5I GGATG 2 cut(s) 28, 118
BstMAI GTCTC 1 cut(s) 291
BstNI CCWGG 3 cut(s) 19, 81, 127
BstSCI CCNGG 3 cut(s) 17, 79, 125
BstV2I GAAGAC 1 cut(s) 68
BsuRI GGCC 1 cut(s) 279
BtsCI GGATG 2 cut(s) 28, 118
Cac8I GCNNGC 1 cut(s) 187
CaiI CAGNNNCTG 1 cut(s) 152
Cfr10I RCCGGY 1 cut(s) 279
Cfr13I GGNCC 2 cut(s) 277, 282
CsiI ACCWGGT 1 cut(s) 125
CviAII CATG 2 cut(s) 231, 260
CviJI RGCY 6 cut(s) 63, 142, 163, 228, 279, 324
CviKI_1 RGCY 6 cut(s) 63, 142, 163, 228, 279, 324
DdeI CTNAG 1 cut(s) 138
Ecl136II GAGCTC 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 165
Eco31I GGTCTC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 163
Eco57I CTGAAG 2 cut(s) 66, 129
Eco88I CYCGRG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 163
EcoRI GAATTC 1 cut(s) 290
EcoRII CCWGG 3 cut(s) 17, 79, 125
EcoT38I GRGCYC 1 cut(s) 165
FaeI CATG 2 cut(s) 234, 263
FaiI YATR 6 cut(s) 12, 232, 237, 261, 329, 340
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 230, 259
FokI GGATG 2 cut(s) 35, 125
FriOI GRGCYC 1 cut(s) 165
FspBI CTAG 1 cut(s) 302
HaeIII GGCC 1 cut(s) 279
HapII CCGG 1 cut(s) 280
Hin1II CATG 2 cut(s) 234, 263
HinfI GANTC 1 cut(s) 155
HpaII CCGG 1 cut(s) 280
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 1 cut(s) 95
Hpy188I TCNGA 2 cut(s) 160, 296
Hpy188III TCNNGA 1 cut(s) 285
Hpy8I GTNNAC 1 cut(s) 95
HpyAV CCTTC 4 cut(s) 20, 153, 193, 215
HpyCH4V TGCA 5 cut(s) 170, 189, 207, 274, 338
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 2 cut(s) 234, 263
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 1 cut(s) 302
MboII GAAGA 1 cut(s) 68
MfeI CAATTG 1 cut(s) 194
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 4 cut(s) 69, 194, 290, 311
MnlI CCTC 2 cut(s) 218, 274
MseI TTAA 1 cut(s) 255
MspI CCGG 1 cut(s) 280
MspR9I CCNGG 3 cut(s) 19, 81, 127
MunI CAATTG 1 cut(s) 194
MvaI CCWGG 3 cut(s) 19, 81, 127
NlaIII CATG 2 cut(s) 234, 263
NlaIV GGNNCC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 155
PshBI ATTAAT 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 165
Psp6I CCWGG 3 cut(s) 17, 79, 125
PspGI CCWGG 3 cut(s) 17, 79, 125
PspN4I GGNNCC 1 cut(s) 284
PspPI GGNCC 2 cut(s) 277, 282
PstNI CAGNNNCTG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 165
SaqAI TTAA 1 cut(s) 255
Sau96I GGNCC 2 cut(s) 277, 282
ScrFI CCNGG 3 cut(s) 19, 81, 127
SduI GDGCHC 1 cut(s) 165
SetI ASST 4 cut(s) 31, 128, 165, 303
SexAI ACCWGGT 1 cut(s) 125
SinI GGWCC 1 cut(s) 282
Sse9I AATT 4 cut(s) 69, 194, 290, 311
SspMI CTAG 1 cut(s) 302
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 3 cut(s) 17, 79, 125
TaqI TCGA 1 cut(s) 6
TasI AATT 4 cut(s) 69, 194, 290, 311
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TspDTI ATGAA 3 cut(s) 39, 69, 322
VpaK11BI GGWCC 1 cut(s) 282
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 2 cut(s) 290, 311
XspI CTAG 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.