RchiOBHm_Chr5g0013371

X8 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000486
Physical Location & Seq
Forward (+)
9039266 .. 9041630
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29391

Sequence Viewer

Length: 519 bp
ATGATGAAGCCTCAAGCGCCGTCGTTCGAAAGTCCTCAACCCCAAGACTTGCTGGGAACACACAAGATTCACAGCTCAGGTGCTTGTGATGTCGAGTTCTCGTTCTGTGGACATTTTCATATTTCACCTTTCAAGGCATTTGCACAGACTTGGTGTGTAGCCAAACCATCATTAGGTCATGATACTCTGGTAAACGTGGAAAGCTATGCATGCAATTTCGCGGATTGCTTTTCAATTCATAGTGGAGATCCATGCTTTGTCACGTCGAACTTGTTTAGTCGTGCATCCTTTGCCATGAATGCTTACTACCAAGAGGAACATGATTGCAAATTCGGTGGCTCTGGACTCAAATCTATTACCGATCCAAGCTATGGGAACTGCATTTTTGTAGGTTCAGAAAAGATGATTTCTCCTCAAGCAGCTCTGGTAATGGATTTTAAGTTTAAATTATTCGCTTTTACTTCTACCAGCTGGTTTCTAACTCTTTGCAGAAGGGTGTTTACATTTGAGCTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

172

Amino Acids

19.17

Weight (kDa)

6.13

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 50 - 115 1.1e-12 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29735 AT4G09090 AT4G09462 AT4G09464 AT4G09465 AT4G09466 AT4G09467 AT5G53600 AT5G53610 AT5G63225 AT5G63230
fragaria_vesca FvH4_3g06020 FvH4_3g06020 FvH4_3g06020 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06040 FvH4_4g08590 FvH4_6g37860
malus_domestica MD10G1265100.v1.1
prunus_persica Prupe.4G076700_v2.0.a1 Prupe.4G076900_v2.0.a1 Prupe.4G084600_v2.0.a1 Prupe.4G130100_v2.0.a1 Prupe.6G294200_v2.0.a1
pyrus_communis pycom05g26640 pycom10g22110
rosa_chinensis RchiOBHm_Chr5g0013281 RchiOBHm_Chr5g0013291 RchiOBHm_Chr5g0013321 RchiOBHm_Chr5g0013341 RchiOBHm_Chr5g0013351 RchiOBHm_Chr5g0013361 RchiOBHm_Chr5g0013371 RchiOBHm_Chr5g0013381 RchiOBHm_Chr5g0013391
rosa_laevigata RLG00000032004 RLG00000032005 RLG00000032008 RLG00000032012 RLG00000032013 RLG00000032014 RLG00000032015 RLG00000032016
rosa_multiflora Rmu_co8200260.1_g000001 Rmu_co8451143.1_g000001 Rmu_sc0003458.1_g000006 Rmu_sc0003458.1_g000008 Rmu_sc0003458.1_g000009 Rmu_sc0003458.1_g000012 Rmu_sc0003458.1_g000014 Rmu_sc0010713.1_g000001
rosa_roxburghii Rroxscaffold_1G00063160 Rroxscaffold_1G00063170 Rroxscaffold_1G00063180 Rroxscaffold_1G00063190 Rroxscaffold_1G00063200 Rroxscaffold_1G00063210
rosa_rugosa Rorug05G0008700 Rorug05G0008700 Rorug05G0008800 Rorug05G0008800 Rorug05G0008900.1 Rorug05G0009000 Rorug05G0009000 Rorug05G0009000
rosa_samantha Rh5AG103700 Rh5AG103800 Rh5AG104000 Rh5AG104300 Rh5AG104400 Rh5BG100300 Rh5BG100400 Rh5BG100500 Rh5BG100600 Rh5BG100700 Rh5CG111500 Rh5CG111600 Rh5CG112000 Rh5CG112200 Rh5CG112300 Rh5CG112500 Rh5DG099200 Rh5DG099300
rosa_wichuraiana Rw5G009040 Rw5G009050 Rw5G009080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 371
AccII CGCG 1 cut(s) 221
AciI CCGC 1 cut(s) 221
AclWI GGATC 2 cut(s) 242, 356
AcsI RAATTY 1 cut(s) 329
AfiI CCNNNNNNNGG 2 cut(s) 173, 371
AgsI TTSAA 2 cut(s) 133, 234
AjiI CACGTC 1 cut(s) 264
AluBI AGCT 6 cut(s) 75, 204, 369, 422, 471, 511
AluI AGCT 6 cut(s) 75, 204, 369, 422, 471, 511
AlwI GGATC 2 cut(s) 242, 356
ApeKI GCWGC 1 cut(s) 419
ApoI RAATTY 1 cut(s) 329
AspLEI GCGC 1 cut(s) 19
AsuHPI GGTGA 1 cut(s) 117
AsuII TTCGAA 1 cut(s) 27
BaeI ACNNNNGTAYC 2 cut(s) 174, 207
BarI GAAGNNNNNNTAC 2 cut(s) 484, 516
BbvI GCAGC 1 cut(s) 431
BccI CCATC 1 cut(s) 175
BceAI ACGGC 1 cut(s) 4
BfoI RGCGCY 1 cut(s) 20
BisI GCNGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 421
BmgBI CACGTC 1 cut(s) 264
BmsI GCATC 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 76
Bpu14I TTCGAA 1 cut(s) 27
BpuEI CTTGAG 1 cut(s) 399
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 371
BseGI GGATG 1 cut(s) 284
BseLI CCNNNNNNNGG 2 cut(s) 173, 371
BseMII CTCAG 1 cut(s) 90
BseRI GAGGAG 1 cut(s) 402
BseXI GCAGC 1 cut(s) 431
BseYI CCCAGC 1 cut(s) 52
Bsh1236I CGCG 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 173, 371
BsmI GAATGC 1 cut(s) 304
Bsp119I TTCGAA 1 cut(s) 27
Bsp143I GATC 2 cut(s) 247, 361
BspACI CCGC 1 cut(s) 221
BspCNI CTCAG 1 cut(s) 89
BspFNI CGCG 1 cut(s) 221
BspHI TCATGA 1 cut(s) 178
BspPI GGATC 2 cut(s) 242, 356
BspT104I TTCGAA 1 cut(s) 27
BssMI GATC 2 cut(s) 247, 361
BstAPI GCANNNNNTGC 1 cut(s) 290
BstBI TTCGAA 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 1 cut(s) 76
BstF5I GGATG 1 cut(s) 284
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 20
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 2 cut(s) 250, 364
BstMBI GATC 2 cut(s) 247, 361
BstMWI GCNNNNNNNGC 4 cut(s) 16, 210, 290, 299
BstNSI RCATGY 1 cut(s) 213
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 431
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
BtrI CACGTC 1 cut(s) 264
BtsCI GGATG 1 cut(s) 284
Cac8I GCNNGC 1 cut(s) 211
CciI TCATGA 1 cut(s) 178
CfoI GCGC 1 cut(s) 19
CspCI CAANNNNNGTGG 2 cut(s) 316, 351
CviAII CATG 5 cut(s) 179, 210, 252, 295, 320
CviJI RGCY 9 cut(s) 10, 75, 161, 204, 339, 369, 422, 471, 511
CviKI_1 RGCY 9 cut(s) 10, 75, 161, 204, 339, 369, 422, 471, 511
DdeI CTNAG 1 cut(s) 76
DpnI GATC 2 cut(s) 249, 363
DpnII GATC 2 cut(s) 247, 361
DraI TTTAAA 1 cut(s) 445
EcoT22I ATGCAT 1 cut(s) 211
FaeI CATG 5 cut(s) 182, 213, 255, 298, 323
FaiI YATR 9 cut(s) 120, 180, 207, 211, 240, 253, 296, 321, 372
FatI CATG 5 cut(s) 178, 209, 251, 294, 319
Fnu4HI GCNGC 1 cut(s) 420
FokI GGATG 1 cut(s) 271
Fsp4HI GCNGC 1 cut(s) 420
GlaI GCGC 1 cut(s) 18
GluI GCNGC 1 cut(s) 420
GsaI CCCAGC 1 cut(s) 56
HaeII RGCGCY 1 cut(s) 20
HhaI GCGC 1 cut(s) 19
Hin1II CATG 5 cut(s) 182, 213, 255, 298, 323
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HinfI GANTC 2 cut(s) 67, 345
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 3 cut(s) 110, 193, 501
Hpy188I TCNGA 1 cut(s) 397
Hpy188III TCNNGA 2 cut(s) 179, 342
Hpy8I GTNNAC 3 cut(s) 110, 193, 501
Hpy99I CGWCG 2 cut(s) 25, 268
HpyAV CCTTC 1 cut(s) 486
HpyCH4IV ACGT 2 cut(s) 195, 263
HpyCH4V TGCA 7 cut(s) 143, 209, 213, 284, 327, 381, 489
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 210, 290, 299
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 2 cut(s) 195, 263
Hsp92II CATG 5 cut(s) 182, 213, 255, 298, 323
HspAI GCGC 1 cut(s) 17
Kzo9I GATC 2 cut(s) 247, 361
LpnPI CCDG 7 cut(s) 38, 63, 173, 327, 410, 457, 481
Lsp1109I GCAGC 1 cut(s) 431
LweI GCATC 1 cut(s) 293
MaeII ACGT 2 cut(s) 195, 263
MaeIII GTNAC 1 cut(s) 259
MalI GATC 2 cut(s) 249, 363
MboI GATC 2 cut(s) 247, 361
MflI RGATCY 1 cut(s) 247
MluCI AATT 4 cut(s) 214, 234, 329, 446
MlyI GAGTC 1 cut(s) 339
MnlI CCTC 4 cut(s) 21, 45, 307, 423
Mph1103I ATGCAT 1 cut(s) 211
MseI TTAA 2 cut(s) 438, 444
MspA1I CMGCKG 1 cut(s) 471
Mva1269I GAATGC 1 cut(s) 304
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 4 cut(s) 16, 210, 290, 299
NdeII GATC 2 cut(s) 247, 361
NlaIII CATG 5 cut(s) 182, 213, 255, 298, 323
NmuCI GTSAC 1 cut(s) 259
NsiI ATGCAT 1 cut(s) 211
NspI RCATGY 1 cut(s) 213
NspV TTCGAA 1 cut(s) 27
PaeI GCATGC 1 cut(s) 213
PagI TCATGA 1 cut(s) 178
PctI GAATGC 1 cut(s) 304
PfeI GAWTC 1 cut(s) 67
PflMI CCANNNNNTGG 1 cut(s) 371
PkrI GCNGC 1 cut(s) 421
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
PspFI CCCAGC 1 cut(s) 52
PsuI RGATCY 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 471
SaqAI TTAA 2 cut(s) 438, 444
SatI GCNGC 1 cut(s) 420
Sau3AI GATC 2 cut(s) 247, 361
SchI GAGTC 1 cut(s) 339
SfaNI GCATC 1 cut(s) 293
SfuI TTCGAA 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 12, 414
SmoI CTYRAG 2 cut(s) 12, 414
SphI GCATGC 1 cut(s) 213
Sse9I AATT 4 cut(s) 214, 234, 329, 446
SsiI CCGC 1 cut(s) 221
TaiI ACGT 2 cut(s) 198, 266
TaqI TCGA 3 cut(s) 27, 93, 266
TasI AATT 4 cut(s) 214, 234, 329, 446
TfiI GAWTC 1 cut(s) 67
Tru1I TTAA 2 cut(s) 438, 444
Tru9I TTAA 2 cut(s) 438, 444
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 1 cut(s) 419
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 4 cut(s) 20, 107, 227, 311
Van91I CCANNNNNTGG 1 cut(s) 371
XapI RAATTY 1 cut(s) 329
XceI RCATGY 1 cut(s) 213
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.