Rmu_sc0003458.1_g000014

X8 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003458.1
OG0000486
Physical Location & Seq
Forward (+)
67091 .. 69105
2015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003458.1_g000014.1.cds

Sequence Viewer

Length: 648 bp
atggcgaatgttcttgtccttcgcactgttgttatcgtggctttcctcgctctctcgaacctcgagctctgcgccattgctgatcttccaggacatggccatgccgctttcatggcaggcattgctaagaatacctggtgtgtggcaaaaccaactgccgctgatagtttgctacaattgaacattgactttgcttgtagtaaagtcaactgtagtgtaattgaacccggtggtgaataccaattaccagacactctaatgaaccacgcgtctgttgccatgaatctttactaccaatcttttggcagaacagatttgagctgttattttcagtcgaccggcatggttgtgattgaagatccaagtaagttctggaacccttgtgtctatgaaggggttgtccttgtgagggatgctgtccctgcaagtatcaaaataaagaagggggaaagaaaggccttttctgcaatatcaattggacttatggtggtgtggtcgatcactggttgtggactggtggcagtggctgtatatgttatgctctggcttcgtcagacccgcgcaccagtggtgggagttcctgtgcacacattgccagagccattgtattcgcggctggagccatcagctcccccgcttcctgtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

215

Amino Acids

23.21

Weight (kDa)

6.88

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29735 AT4G09090 AT4G09462 AT4G09464 AT4G09465 AT4G09466 AT4G09467 AT5G53600 AT5G53610 AT5G63225 AT5G63230
fragaria_vesca FvH4_3g06020 FvH4_3g06020 FvH4_3g06020 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06040 FvH4_4g08590 FvH4_6g37860
malus_domestica MD10G1265100.v1.1
prunus_persica Prupe.4G076700_v2.0.a1 Prupe.4G076900_v2.0.a1 Prupe.4G084600_v2.0.a1 Prupe.4G130100_v2.0.a1 Prupe.6G294200_v2.0.a1
pyrus_communis pycom05g26640 pycom10g22110
rosa_chinensis RchiOBHm_Chr5g0013281 RchiOBHm_Chr5g0013291 RchiOBHm_Chr5g0013321 RchiOBHm_Chr5g0013341 RchiOBHm_Chr5g0013351 RchiOBHm_Chr5g0013361 RchiOBHm_Chr5g0013371 RchiOBHm_Chr5g0013381 RchiOBHm_Chr5g0013391
rosa_laevigata RLG00000032004 RLG00000032005 RLG00000032008 RLG00000032012 RLG00000032013 RLG00000032014 RLG00000032015 RLG00000032016
rosa_multiflora Rmu_co8200260.1_g000001 Rmu_co8451143.1_g000001 Rmu_sc0003458.1_g000006 Rmu_sc0003458.1_g000008 Rmu_sc0003458.1_g000009 Rmu_sc0003458.1_g000012 Rmu_sc0003458.1_g000014 Rmu_sc0010713.1_g000001
rosa_roxburghii Rroxscaffold_1G00063160 Rroxscaffold_1G00063170 Rroxscaffold_1G00063180 Rroxscaffold_1G00063190 Rroxscaffold_1G00063200 Rroxscaffold_1G00063210
rosa_rugosa Rorug05G0008700 Rorug05G0008700 Rorug05G0008800 Rorug05G0008800 Rorug05G0008900.1 Rorug05G0009000 Rorug05G0009000 Rorug05G0009000
rosa_samantha Rh5AG103700 Rh5AG103800 Rh5AG104000 Rh5AG104300 Rh5AG104400 Rh5BG100300 Rh5BG100400 Rh5BG100500 Rh5BG100600 Rh5BG100700 Rh5CG111500 Rh5CG111600 Rh5CG112000 Rh5CG112200 Rh5CG112300 Rh5CG112500 Rh5DG099200 Rh5DG099300
rosa_wichuraiana Rw5G009040 Rw5G009050 Rw5G009080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AccI GTMKAC 1 cut(s) 335
AccII CGCG 3 cut(s) 269, 561, 613
AciI CCGC 5 cut(s) 105, 159, 559, 613, 635
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 1 cut(s) 97
AfiI CCNNNNNNNGG 3 cut(s) 95, 409, 572
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 3 cut(s) 181, 224, 356
AjnI CCWGG 2 cut(s) 88, 134
AluBI AGCT 3 cut(s) 67, 321, 629
AluI AGCT 3 cut(s) 67, 321, 629
Alw21I GWGCWC 2 cut(s) 69, 588
Alw44I GTGCAC 1 cut(s) 584
AlwI GGATC 1 cut(s) 353
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 2 cut(s) 97, 456
ApaLI GTGCAC 1 cut(s) 584
AspLEI GCGC 2 cut(s) 74, 563
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 62
BaeGI GKGCMC 1 cut(s) 588
BalI TGGCCA 1 cut(s) 99
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 69, 588
BccI CCATC 1 cut(s) 631
BciT130I CCWGG 2 cut(s) 90, 136
BcnI CCSGG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 211
BisI GCNGC 3 cut(s) 105, 159, 614
BlsI GCNGC 3 cut(s) 106, 160, 615
Bme1390I CCNGG 3 cut(s) 90, 136, 228
BmeT110I CYCGRG 1 cut(s) 62
BmiI GGNNCC 2 cut(s) 377, 621
BmrFI CCNGG 3 cut(s) 90, 136, 228
BmsI GCATC 1 cut(s) 403
BpmI CTGGAG 1 cut(s) 638
BpuMI CCSGG 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 409, 572
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 3 cut(s) 508, 519, 566
Bse3DI GCAATG 3 cut(s) 75, 120, 590
BseBI CCWGG 2 cut(s) 90, 136
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 3 cut(s) 95, 409, 572
BseMI GCAATG 3 cut(s) 75, 120, 590
BseNI ACTGG 3 cut(s) 508, 519, 566
BseSI GKGCMC 1 cut(s) 588
Bsh1236I CGCG 3 cut(s) 269, 561, 613
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 2 cut(s) 99, 458
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 2 cut(s) 69, 588
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 2 cut(s) 228, 339
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 3 cut(s) 95, 409, 572
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 2 cut(s) 99, 458
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 2 cut(s) 69, 588
Bsp143I GATC 3 cut(s) 82, 358, 498
BspACI CCGC 5 cut(s) 105, 159, 559, 613, 635
BspANI GGCC 2 cut(s) 99, 458
BspFNI CGCG 3 cut(s) 269, 561, 613
BspLI GGNNCC 2 cut(s) 377, 621
BspPI GGATC 1 cut(s) 353
BsrDI GCAATG 3 cut(s) 75, 120, 590
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 3 cut(s) 508, 519, 566
BssAI RCCGGY 1 cut(s) 338
BssMI GATC 3 cut(s) 82, 358, 498
Bst2UI CCWGG 2 cut(s) 90, 136
Bst4CI ACNGT 2 cut(s) 28, 212
BstAPI GCANNNNNTGC 2 cut(s) 122, 592
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 3 cut(s) 269, 561, 613
BstHHI GCGC 2 cut(s) 74, 563
BstKTI GATC 3 cut(s) 85, 361, 501
BstMBI GATC 3 cut(s) 82, 358, 498
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 8 cut(s) 47, 113, 122, 275, 422, 464, 592, 619
BstNI CCWGG 2 cut(s) 90, 136
BstSCI CCNGG 3 cut(s) 88, 134, 226
BstSFI CTRYAG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 588
BstUI CGCG 3 cut(s) 269, 561, 613
BstX2I RGATCY 1 cut(s) 358
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 2 cut(s) 99, 458
BtsCI GGATG 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 4 cut(s) 24, 501, 528, 573
Cac8I GCNNGC 1 cut(s) 118
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 2 cut(s) 74, 563
Cfr10I RCCGGY 1 cut(s) 338
CseI GACGC 1 cut(s) 258
CsiI ACCWGGT 1 cut(s) 134
CviAII CATG 5 cut(s) 95, 101, 112, 280, 343
DdeI CTNAG 1 cut(s) 126
DpnI GATC 3 cut(s) 84, 360, 500
DpnII GATC 3 cut(s) 82, 358, 498
EaeI YGGCCR 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 67
Eco147I AGGCCT 1 cut(s) 458
Eco24I GRGCYC 1 cut(s) 69
Eco53kI GAGCTC 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 88, 134
EcoT38I GRGCYC 1 cut(s) 69
FaeI CATG 5 cut(s) 98, 104, 115, 283, 346
FaqI GGGAC 1 cut(s) 404
FatI CATG 5 cut(s) 94, 100, 111, 279, 342
FauI CCCGC 2 cut(s) 566, 642
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 3 cut(s) 105, 159, 614
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 3 cut(s) 105, 159, 614
GlaI GCGC 2 cut(s) 73, 562
GluI GCNGC 3 cut(s) 105, 159, 614
GsuI CTGGAG 1 cut(s) 638
HaeIII GGCC 2 cut(s) 99, 458
HapII CCGG 2 cut(s) 228, 339
HgaI GACGC 1 cut(s) 258
HhaI GCGC 2 cut(s) 74, 563
Hin1II CATG 5 cut(s) 98, 104, 115, 283, 346
Hin6I GCGC 2 cut(s) 72, 561
HinP1I GCGC 2 cut(s) 72, 561
HincII GTYRAC 2 cut(s) 208, 336
HindII GTYRAC 2 cut(s) 208, 336
HinfI GANTC 1 cut(s) 283
HpaII CCGG 2 cut(s) 228, 339
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 4 cut(s) 208, 336, 512, 586
Hpy188I TCNGA 1 cut(s) 555
Hpy188III TCNNGA 2 cut(s) 55, 373
Hpy8I GTNNAC 4 cut(s) 208, 336, 512, 586
HpyAV CCTTC 3 cut(s) 29, 386, 436
HpyCH4III ACNGT 2 cut(s) 28, 212
HpyCH4V TGCA 3 cut(s) 425, 467, 586
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 113, 122, 275, 422, 464, 592, 619
HpyF3I CTNAG 1 cut(s) 126
Hsp92II CATG 5 cut(s) 98, 104, 115, 283, 346
HspAI GCGC 2 cut(s) 72, 561
Kzo9I GATC 3 cut(s) 82, 358, 498
LmnI GCTCC 2 cut(s) 619, 634
LweI GCATC 1 cut(s) 403
MabI ACCWGGT 1 cut(s) 134
MalI GATC 3 cut(s) 84, 360, 500
MboI GATC 3 cut(s) 82, 358, 498
MboII GAAGA 2 cut(s) 77, 368
MfeI CAATTG 2 cut(s) 176, 474
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 2 cut(s) 69, 588
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 4 cut(s) 176, 219, 242, 474
MluI ACGCGT 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 99
MnlI CCTC 3 cut(s) 56, 71, 402
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MslI CAYNNNNRTG 3 cut(s) 99, 257, 347
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 228, 339
MspR9I CCNGG 3 cut(s) 90, 136, 228
MunI CAATTG 2 cut(s) 176, 474
MvaI CCWGG 2 cut(s) 90, 136
MvnI CGCG 3 cut(s) 269, 561, 613
MwoI GCNNNNNNNGC 8 cut(s) 47, 113, 122, 275, 422, 464, 592, 619
NciI CCSGG 1 cut(s) 228
NdeII GATC 3 cut(s) 82, 358, 498
NlaIII CATG 5 cut(s) 98, 104, 115, 283, 346
NlaIV GGNNCC 2 cut(s) 377, 621
PaeR7I CTCGAG 1 cut(s) 62
PceI AGGCCT 1 cut(s) 458
PfeI GAWTC 1 cut(s) 283
PflMI CCANNNNNTGG 1 cut(s) 95
PfoI TCCNGGA 1 cut(s) 88
PkrI GCNGC 3 cut(s) 106, 160, 615
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 88, 134
PspGI CCWGG 2 cut(s) 88, 134
PspN4I GGNNCC 2 cut(s) 377, 621
PspXI VCTCGAGB 1 cut(s) 62
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 1 cut(s) 358
RseI CAYNNNNRTG 3 cut(s) 99, 257, 347
SacI GAGCTC 1 cut(s) 69
SalI GTCGAC 1 cut(s) 334
SatI GCNGC 3 cut(s) 105, 159, 614
Sau3AI GATC 3 cut(s) 82, 358, 498
ScrFI CCNGG 3 cut(s) 90, 136, 228
SduI GDGCHC 2 cut(s) 69, 588
SetI ASST 5 cut(s) 63, 69, 137, 323, 631
SexAI ACCWGGT 1 cut(s) 134
SfaNI GCATC 1 cut(s) 403
SfcI CTRYAG 1 cut(s) 211
Sfr274I CTCGAG 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 62
SmiMI CAYNNNNRTG 3 cut(s) 99, 257, 347
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 4 cut(s) 176, 219, 242, 474
SseBI AGGCCT 1 cut(s) 458
SsiI CCGC 5 cut(s) 105, 159, 559, 613, 635
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 458
StyD4I CCNGG 3 cut(s) 88, 134, 226
TaaI ACNGT 2 cut(s) 28, 212
TaqI TCGA 4 cut(s) 56, 63, 335, 497
TasI AATT 4 cut(s) 176, 219, 242, 474
TauI GCSGC 3 cut(s) 107, 161, 616
TfiI GAWTC 1 cut(s) 283
TscAI CASTG 4 cut(s) 31, 508, 528, 573
TspDTI ATGAA 4 cut(s) 100, 275, 296, 405
TspRI CASTG 4 cut(s) 31, 508, 528, 573
Van91I CCANNNNNTGG 1 cut(s) 95
VneI GTGCAC 1 cut(s) 584
XcmI CCANNNNNNNNNTGG 1 cut(s) 369
XhoI CTCGAG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.