Rmu_sc0003458.1_g000009

X8 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003458.1
OG0000486
Physical Location & Seq
Forward (+)
38154 .. 40337
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003458.1_g000009.1.cds

Sequence Viewer

Length: 687 bp
atgcgcctctcaatggtgttcatcgccctccaattccaattctatgctgcaatggccaagtctatttccctctgcacacttgcagcctctctgctccttctgcttcatgccaatgctgcttctccaggagatggtcatactccatccactgaggagattgccaagagtacatggtgtgttgcaaagccaactgccactgacaatcttctacaattgaacattgacttcgcttgtagtcatgtcaactgtagtgttattgaaccccgtggtgaatgccaattaccagacgctataatgaaccacgcatctgttgccatgaatctttactatcaatcttttggcagaacagatatgagctgttattttcagtccacaggcatggttgtgattgaagatccaagttctggaacttgtgtctatgaaggggttttccttgacagcgaacctgtgactcctgtccctgcaaaagggaaaagtagggccttttctgcaacatcagttggagttatggtgatggggtcgatcattggttgtgcgctggtggcagtggctatatatgttatgctctggcttcgtcagccccgcccgccagtggtaaaggaagttcacatggacccattgccacagccattgtatcaaccaccagagccatcagcttccccagctcctgttgagggttcttattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

228

Amino Acids

24.51

Weight (kDa)

5.26

Isoelectric Point (pI)

60.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29735 AT4G09090 AT4G09462 AT4G09464 AT4G09465 AT4G09466 AT4G09467 AT5G53600 AT5G53610 AT5G63225 AT5G63230
fragaria_vesca FvH4_3g06020 FvH4_3g06020 FvH4_3g06020 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06040 FvH4_4g08590 FvH4_6g37860
malus_domestica MD10G1265100.v1.1
prunus_persica Prupe.4G076700_v2.0.a1 Prupe.4G076900_v2.0.a1 Prupe.4G084600_v2.0.a1 Prupe.4G130100_v2.0.a1 Prupe.6G294200_v2.0.a1
pyrus_communis pycom05g26640 pycom10g22110
rosa_chinensis RchiOBHm_Chr5g0013281 RchiOBHm_Chr5g0013291 RchiOBHm_Chr5g0013321 RchiOBHm_Chr5g0013341 RchiOBHm_Chr5g0013351 RchiOBHm_Chr5g0013361 RchiOBHm_Chr5g0013371 RchiOBHm_Chr5g0013381 RchiOBHm_Chr5g0013391
rosa_laevigata RLG00000032004 RLG00000032005 RLG00000032008 RLG00000032012 RLG00000032013 RLG00000032014 RLG00000032015 RLG00000032016
rosa_multiflora Rmu_co8200260.1_g000001 Rmu_co8451143.1_g000001 Rmu_sc0003458.1_g000006 Rmu_sc0003458.1_g000008 Rmu_sc0003458.1_g000009 Rmu_sc0003458.1_g000012 Rmu_sc0003458.1_g000014 Rmu_sc0010713.1_g000001
rosa_roxburghii Rroxscaffold_1G00063160 Rroxscaffold_1G00063170 Rroxscaffold_1G00063180 Rroxscaffold_1G00063190 Rroxscaffold_1G00063200 Rroxscaffold_1G00063210
rosa_rugosa Rorug05G0008700 Rorug05G0008700 Rorug05G0008800 Rorug05G0008800 Rorug05G0008900.1 Rorug05G0009000 Rorug05G0009000 Rorug05G0009000
rosa_samantha Rh5AG103700 Rh5AG103800 Rh5AG104000 Rh5AG104300 Rh5AG104400 Rh5BG100300 Rh5BG100400 Rh5BG100500 Rh5BG100600 Rh5BG100700 Rh5CG111500 Rh5CG111600 Rh5CG112000 Rh5CG112200 Rh5CG112300 Rh5CG112500 Rh5DG099200 Rh5DG099300
rosa_wichuraiana Rw5G009040 Rw5G009050 Rw5G009080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 131, 404
AciI CCGC 2 cut(s) 583, 587
AclWI GGATC 1 cut(s) 389
AcoI YGGCCR 1 cut(s) 54
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 6 cut(s) 13, 131, 404, 467, 592, 674
AgsI TTSAA 3 cut(s) 217, 260, 392
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 3 cut(s) 357, 656, 665
AluI AGCT 3 cut(s) 357, 656, 665
AlwI GGATC 1 cut(s) 389
AlwNI CAGNNNCTG 1 cut(s) 668
AoxI GGCC 2 cut(s) 54, 480
ApeKI GCWGC 3 cut(s) 47, 83, 116
AspLEI GCGC 2 cut(s) 6, 538
AspS9I GGNCC 2 cut(s) 480, 613
AsuHPI GGTGA 2 cut(s) 281, 523
AvaII GGWCC 1 cut(s) 613
BalI TGGCCA 1 cut(s) 56
BbvI GCAGC 3 cut(s) 34, 95, 103
BccI CCATC 4 cut(s) 125, 151, 508, 658
BciT130I CCWGG 1 cut(s) 126
BfmI CTRYAG 1 cut(s) 247
BisI GCNGC 3 cut(s) 48, 84, 117
BlsI GCNGC 3 cut(s) 49, 85, 118
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 1 cut(s) 613
BmgT120I GGNCC 2 cut(s) 480, 613
BmiI GGNNCC 1 cut(s) 615
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 1 cut(s) 314
BoxI GACNNNNGTC 1 cut(s) 455
BpmI CTGGAG 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 265
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 6 cut(s) 13, 131, 404, 467, 592, 674
Bse1I ACTGG 1 cut(s) 590
Bse3DI GCAATG 2 cut(s) 57, 617
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 1 cut(s) 143
BseLI CCNNNNNNNGG 6 cut(s) 13, 131, 404, 467, 592, 674
BseMI GCAATG 2 cut(s) 57, 617
BseMII CTCAG 1 cut(s) 141
BseNI ACTGG 1 cut(s) 590
BseRI GAGGAG 1 cut(s) 167
BseXI GCAGC 3 cut(s) 34, 95, 103
BseYI CCCAGC 1 cut(s) 661
BsgI GTGCAG 1 cut(s) 58
BshFI GGCC 2 cut(s) 56, 482
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 6 cut(s) 13, 131, 404, 467, 592, 674
BsmFI GGGAC 1 cut(s) 443
BsmI GAATGC 1 cut(s) 278
BsnI GGCC 2 cut(s) 56, 482
Bsp143I GATC 2 cut(s) 394, 522
BspACI CCGC 2 cut(s) 583, 587
BspANI GGCC 2 cut(s) 56, 482
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 615
BspPI GGATC 1 cut(s) 389
BsrDI GCAATG 2 cut(s) 57, 617
BsrI ACTGG 1 cut(s) 590
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 2 cut(s) 394, 522
Bst2UI CCWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 248
BstAPI GCANNNNNTGC 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 587
BstDEI CTNAG 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 465
BstF5I GGATG 1 cut(s) 143
BstHHI GCGC 2 cut(s) 6, 538
BstKTI GATC 2 cut(s) 397, 525
BstMBI GATC 2 cut(s) 394, 522
BstMWI GCNNNNNNNGC 9 cut(s) 53, 100, 116, 311, 488, 542, 577, 586, 662
BstNI CCWGG 1 cut(s) 126
BstPAI GACNNNNGTC 1 cut(s) 455
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 1 cut(s) 247
BstV1I GCAGC 3 cut(s) 34, 95, 103
BstX2I RGATCY 1 cut(s) 394
BstXI CCANNNNNNTGG 1 cut(s) 379
BstYI RGATCY 1 cut(s) 394
BsuRI GGCC 2 cut(s) 56, 482
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 1 cut(s) 143
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 4 cut(s) 147, 195, 552, 597
Cac8I GCNNGC 1 cut(s) 587
CaiI CAGNNNCTG 1 cut(s) 668
CfoI GCGC 2 cut(s) 6, 538
Cfr13I GGNCC 2 cut(s) 480, 613
CseI GACGC 1 cut(s) 296
Csp6I GTAC 1 cut(s) 168
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviAII CATG 6 cut(s) 107, 171, 239, 316, 379, 610
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 1 cut(s) 150
DpnI GATC 2 cut(s) 396, 524
DpnII GATC 2 cut(s) 394, 522
EaeI YGGCCR 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 613
EcoNI CCTNNNNNAGG 1 cut(s) 465
EcoO109I RGGNCCY 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 124
FaeI CATG 6 cut(s) 110, 174, 242, 319, 382, 613
FaqI GGGAC 1 cut(s) 443
FatI CATG 6 cut(s) 106, 170, 238, 315, 378, 609
FauI CCCGC 2 cut(s) 590, 594
Fnu4HI GCNGC 3 cut(s) 48, 84, 117
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 3 cut(s) 48, 84, 117
GlaI GCGC 2 cut(s) 5, 537
GluI GCNGC 3 cut(s) 48, 84, 117
GsaI CCCAGC 1 cut(s) 665
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 2 cut(s) 56, 482
HgaI GACGC 1 cut(s) 296
HhaI GCGC 2 cut(s) 6, 538
Hin1II CATG 6 cut(s) 110, 174, 242, 319, 382, 613
Hin6I GCGC 2 cut(s) 4, 536
HinP1I GCGC 2 cut(s) 4, 536
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 2 cut(s) 319, 451
HphI GGTGA 2 cut(s) 281, 523
Hpy166II GTNNAC 3 cut(s) 244, 372, 607
Hpy188III TCNNGA 1 cut(s) 405
Hpy8I GTNNAC 3 cut(s) 244, 372, 607
HpyAV CCTTC 2 cut(s) 107, 416
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4V TGCA 6 cut(s) 50, 75, 83, 182, 464, 491
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 100, 116, 311, 488, 542, 577, 586, 662
HpyF3I CTNAG 1 cut(s) 150
Hsp92II CATG 6 cut(s) 110, 174, 242, 319, 382, 613
HspAI GCGC 2 cut(s) 4, 536
Kzo9I GATC 2 cut(s) 394, 522
LmnI GCTCC 2 cut(s) 99, 670
Lsp1109I GCAGC 3 cut(s) 34, 95, 103
LweI GCATC 1 cut(s) 314
MaeIII GTNAC 1 cut(s) 448
MalI GATC 2 cut(s) 396, 524
MboI GATC 2 cut(s) 394, 522
MboII GAAGA 2 cut(s) 197, 404
MfeI CAATTG 1 cut(s) 212
MflI RGATCY 1 cut(s) 394
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 4 cut(s) 32, 38, 212, 278
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 1 cut(s) 445
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 6 cut(s) 17, 38, 80, 97, 145, 667
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MslI CAYNNNNRTG 3 cut(s) 111, 377, 383
Msp20I TGGCCA 1 cut(s) 56
MspR9I CCNGG 1 cut(s) 126
MunI CAATTG 1 cut(s) 212
Mva1269I GAATGC 1 cut(s) 278
MvaI CCWGG 1 cut(s) 126
MwoI GCNNNNNNNGC 9 cut(s) 53, 100, 116, 311, 488, 542, 577, 586, 662
NdeII GATC 2 cut(s) 394, 522
NlaIII CATG 6 cut(s) 110, 174, 242, 319, 382, 613
NlaIV GGNNCC 1 cut(s) 615
NmuCI GTSAC 1 cut(s) 448
PctI GAATGC 1 cut(s) 278
PfeI GAWTC 1 cut(s) 319
PflMI CCANNNNNTGG 2 cut(s) 131, 404
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 3 cut(s) 49, 85, 118
PleI GAGTC 1 cut(s) 445
PpsI GAGTC 1 cut(s) 445
PshAI GACNNNNGTC 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 124
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 615
PspPI GGNCC 2 cut(s) 480, 613
PsrI GAACNNNNNNTAC 2 cut(s) 588, 620
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 1 cut(s) 394
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 3 cut(s) 111, 377, 383
SatI GCNGC 3 cut(s) 48, 84, 117
Sau3AI GATC 2 cut(s) 394, 522
Sau96I GGNCC 2 cut(s) 480, 613
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 1 cut(s) 126
SetI ASST 4 cut(s) 359, 448, 658, 667
SfaNI GCATC 1 cut(s) 314
SfcI CTRYAG 1 cut(s) 247
SinI GGWCC 1 cut(s) 613
SmiMI CAYNNNNRTG 3 cut(s) 111, 377, 383
Sse9I AATT 4 cut(s) 32, 38, 212, 278
SsiI CCGC 2 cut(s) 583, 587
StyD4I CCNGG 1 cut(s) 124
TaaI ACNGT 1 cut(s) 248
TaqI TCGA 1 cut(s) 521
TasI AATT 4 cut(s) 32, 38, 212, 278
TatI WGTACW 1 cut(s) 167
TfiI GAWTC 1 cut(s) 319
TscAI CASTG 4 cut(s) 154, 202, 552, 597
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 3 cut(s) 47, 83, 116
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 5 cut(s) 10, 95, 311, 332, 435
TspRI CASTG 4 cut(s) 154, 202, 552, 597
Van91I CCANNNNNTGG 2 cut(s) 131, 404
VpaK11BI GGWCC 1 cut(s) 613
XagI CCTNNNNNAGG 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.