Rw5G009080

Glucan endo-1,3-beta-glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000486
Physical Location & Seq
Forward (+)
9777062 .. 9798934
21873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G009080.1

Sequence Viewer

Length: 468 bp
ATGGCTCAATCAAGTAGTGCTTCATCTCTTTCCATTCTGTTCCTTCTGCTCTTGTTCATCCACTTTCCTGGGCCTATAAAGCTTGTTAGTTGGGATGAGAATAAGACCTGGTGTGCTGCTAAGCCTTCAGCAACTGATTCAGAGCTTCTGCAAACATCCAGTTTGCCTAATACCTGGTGTGTGGCAAAACCAACTGCCGCTGATAGTTTGCTACAATTGAACATTGACTTTGCTTGTAGTAAATTCAACTGTAGTGTAATTGAACCCGGTGGTGAATGCCAATTACCAGACACTCTAATGAACCACGCGTCTGTTGCCATGAATCTTTACTACCAATCTTTTGGCAGAACAGATTTGAGCTGTTATTTTCAGTCGACCGGCATGGTTGTGATTGAAGATCCAACTGGTTATGGTAGCTGCAAATACAACCATAACAAATGCAGTATCGGGTGGTTTGTCGAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

155

Amino Acids

17.01

Weight (kDa)

4.95

Isoelectric Point (pI)

55.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 58 - 128 5.1e-18 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29735 AT4G09090 AT4G09462 AT4G09464 AT4G09465 AT4G09466 AT4G09467 AT5G53600 AT5G53610 AT5G63225 AT5G63230
fragaria_vesca FvH4_3g06020 FvH4_3g06020 FvH4_3g06020 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06040 FvH4_4g08590 FvH4_6g37860
malus_domestica MD10G1265100.v1.1
prunus_persica Prupe.4G076700_v2.0.a1 Prupe.4G076900_v2.0.a1 Prupe.4G084600_v2.0.a1 Prupe.4G130100_v2.0.a1 Prupe.6G294200_v2.0.a1
pyrus_communis pycom05g26640 pycom10g22110
rosa_chinensis RchiOBHm_Chr5g0013281 RchiOBHm_Chr5g0013291 RchiOBHm_Chr5g0013321 RchiOBHm_Chr5g0013341 RchiOBHm_Chr5g0013351 RchiOBHm_Chr5g0013361 RchiOBHm_Chr5g0013371 RchiOBHm_Chr5g0013381 RchiOBHm_Chr5g0013391
rosa_laevigata RLG00000032004 RLG00000032005 RLG00000032008 RLG00000032012 RLG00000032013 RLG00000032014 RLG00000032015 RLG00000032016
rosa_multiflora Rmu_co8200260.1_g000001 Rmu_co8451143.1_g000001 Rmu_sc0003458.1_g000006 Rmu_sc0003458.1_g000008 Rmu_sc0003458.1_g000009 Rmu_sc0003458.1_g000012 Rmu_sc0003458.1_g000014 Rmu_sc0010713.1_g000001
rosa_roxburghii Rroxscaffold_1G00063160 Rroxscaffold_1G00063170 Rroxscaffold_1G00063180 Rroxscaffold_1G00063190 Rroxscaffold_1G00063200 Rroxscaffold_1G00063210
rosa_rugosa Rorug05G0008700 Rorug05G0008700 Rorug05G0008800 Rorug05G0008800 Rorug05G0008900.1 Rorug05G0009000 Rorug05G0009000 Rorug05G0009000
rosa_samantha Rh5AG103700 Rh5AG103800 Rh5AG104000 Rh5AG104300 Rh5AG104400 Rh5BG100300 Rh5BG100400 Rh5BG100500 Rh5BG100600 Rh5BG100700 Rh5CG111500 Rh5CG111600 Rh5CG112000 Rh5CG112200 Rh5CG112300 Rh5CG112500 Rh5DG099200 Rh5DG099300
rosa_wichuraiana Rw5G009040 Rw5G009050 Rw5G009080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 374
AccII CGCG 1 cut(s) 308
AciI CCGC 1 cut(s) 198
AclWI GGATC 1 cut(s) 392
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 111
AflIII ACRYGT 1 cut(s) 306
AgsI TTSAA 4 cut(s) 220, 247, 263, 395
AjnI CCWGG 3 cut(s) 67, 107, 173
AluBI AGCT 4 cut(s) 82, 145, 360, 417
AluI AGCT 4 cut(s) 82, 145, 360, 417
AlwI GGATC 1 cut(s) 392
AlwNI CAGNNNCTG 1 cut(s) 134
AoxI GGCC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 116, 417
ApoI RAATTY 1 cut(s) 242
AspS9I GGNCC 1 cut(s) 71
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 1 cut(s) 284
BbvI GCAGC 2 cut(s) 103, 404
BciT130I CCWGG 3 cut(s) 69, 109, 175
BcnI CCSGG 1 cut(s) 267
BfmI CTRYAG 1 cut(s) 250
BisI GCNGC 3 cut(s) 117, 198, 418
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 3 cut(s) 118, 199, 419
Bme1390I CCNGG 4 cut(s) 69, 109, 175, 267
BmgT120I GGNCC 1 cut(s) 71
BmrFI CCNGG 4 cut(s) 69, 109, 175, 267
Bpu1102I GCTNAGC 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 68
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 2 cut(s) 159, 409
BseBI CCWGG 3 cut(s) 69, 109, 175
BseDI CCNNGG 1 cut(s) 68
BseGI GGATG 3 cut(s) 57, 100, 155
BseNI ACTGG 2 cut(s) 159, 409
BseXI GCAGC 2 cut(s) 103, 404
Bsh1236I CGCG 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 378
BshFI GGCC 1 cut(s) 73
BsiEI CGRYCG 1 cut(s) 378
BsiSI CCGG 2 cut(s) 267, 378
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 1 cut(s) 73
Bsp143I GATC 1 cut(s) 397
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 1 cut(s) 198
BspANI GGCC 1 cut(s) 73
BspFNI CGCG 1 cut(s) 308
BspPI GGATC 1 cut(s) 392
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 2 cut(s) 159, 409
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 1 cut(s) 68
BssMI GATC 1 cut(s) 397
Bst2UI CCWGG 3 cut(s) 69, 109, 175
Bst4CI ACNGT 1 cut(s) 251
BstDEI CTNAG 1 cut(s) 120
BstF5I GGATG 3 cut(s) 57, 100, 155
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 1 cut(s) 400
BstMBI GATC 1 cut(s) 397
BstMCI CGRYCG 1 cut(s) 378
BstMWI GCNNNNNNNGC 2 cut(s) 79, 314
BstNI CCWGG 3 cut(s) 69, 109, 175
BstSCI CCNGG 4 cut(s) 67, 107, 173, 265
BstSFI CTRYAG 1 cut(s) 250
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 2 cut(s) 103, 404
BstX2I RGATCY 1 cut(s) 397
BstXI CCANNNNNNTGG 2 cut(s) 68, 341
BstYI RGATCY 1 cut(s) 397
BsuRI GGCC 1 cut(s) 73
BtsCI GGATG 3 cut(s) 57, 100, 155
CaiI CAGNNNCTG 1 cut(s) 134
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 1 cut(s) 71
CseI GACGC 1 cut(s) 297
CsiI ACCWGGT 2 cut(s) 107, 173
CviAII CATG 3 cut(s) 319, 382, 465
CviJI RGCY 7 cut(s) 5, 73, 82, 124, 145, 360, 417
CviKI_1 RGCY 7 cut(s) 5, 73, 82, 124, 145, 360, 417
DdeI CTNAG 1 cut(s) 120
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 111
EcoRII CCWGG 3 cut(s) 67, 107, 173
FaeI CATG 3 cut(s) 322, 385, 468
FaiI YATR 6 cut(s) 77, 320, 383, 411, 432, 466
FalI AAGNNNNNCTT 1 cut(s) 36
FatI CATG 3 cut(s) 318, 381, 464
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 3 cut(s) 117, 198, 418
FokI GGATG 3 cut(s) 44, 107, 142
Fsp4HI GCNGC 3 cut(s) 117, 198, 418
GluI GCNGC 3 cut(s) 117, 198, 418
HaeIII GGCC 1 cut(s) 73
HapII CCGG 2 cut(s) 267, 378
HgaI GACGC 1 cut(s) 297
Hin1II CATG 3 cut(s) 322, 385, 468
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 2 cut(s) 137, 322
HpaII CCGG 2 cut(s) 267, 378
HphI GGTGA 1 cut(s) 284
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 1 cut(s) 142
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 2 cut(s) 53, 135
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4V TGCA 3 cut(s) 151, 420, 441
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 314
HpyF3I CTNAG 1 cut(s) 120
Hsp92II CATG 3 cut(s) 322, 385, 468
Kzo9I GATC 1 cut(s) 397
Lsp1109I GCAGC 2 cut(s) 103, 404
MabI ACCWGGT 2 cut(s) 107, 173
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MboII GAAGA 1 cut(s) 407
MfeI CAATTG 1 cut(s) 215
MflI RGATCY 1 cut(s) 397
MluCI AATT 4 cut(s) 215, 242, 258, 281
MluI ACGCGT 1 cut(s) 306
MmeI TCCRAC 1 cut(s) 425
MslI CAYNNNNRTG 2 cut(s) 296, 386
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 2 cut(s) 267, 378
MspR9I CCNGG 4 cut(s) 69, 109, 175, 267
MunI CAATTG 1 cut(s) 215
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 3 cut(s) 69, 109, 175
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 2 cut(s) 79, 314
NciI CCSGG 1 cut(s) 267
NdeII GATC 1 cut(s) 397
NlaIII CATG 3 cut(s) 322, 385, 468
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 137, 322
PkrI GCNGC 3 cut(s) 118, 199, 419
Psp6I CCWGG 3 cut(s) 67, 107, 173
PspGI CCWGG 3 cut(s) 67, 107, 173
PspPI GGNCC 1 cut(s) 71
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 1 cut(s) 397
RseI CAYNNNNRTG 2 cut(s) 296, 386
SalI GTCGAC 1 cut(s) 373
SatI GCNGC 3 cut(s) 117, 198, 418
Sau3AI GATC 1 cut(s) 397
Sau96I GGNCC 1 cut(s) 71
ScrFI CCNGG 4 cut(s) 69, 109, 175, 267
SetI ASST 6 cut(s) 84, 110, 147, 176, 362, 419
SexAI ACCWGGT 2 cut(s) 107, 173
SfcI CTRYAG 1 cut(s) 250
SmiMI CAYNNNNRTG 2 cut(s) 296, 386
Sse9I AATT 4 cut(s) 215, 242, 258, 281
SsiI CCGC 1 cut(s) 198
StyD4I CCNGG 4 cut(s) 67, 107, 173, 265
TaaI ACNGT 1 cut(s) 251
TaqI TCGA 2 cut(s) 374, 459
TasI AATT 4 cut(s) 215, 242, 258, 281
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 2 cut(s) 137, 322
TseI GCWGC 2 cut(s) 116, 417
TspDTI ATGAA 4 cut(s) 12, 46, 314, 335
XapI RAATTY 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.