RchiOBHm_Chr5g0013391

X8 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000486
Physical Location & Seq
Forward (+)
9048007 .. 9050977
2971 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29393

Sequence Viewer

Length: 642 bp
ATGGCGAATGTTCTTGTCCTTCGCACTGTTGTTATCGTGGCCTTCCTCGCTCTCTCGAACCTCGAGCTCTGCGCCATTGCTGATCTTCCAGGACATGGCCATGCCGCTTTCATGGCAGACATTGCTAAGAATACCTGGTGTGTGGCAAAGCCAACTGCCGCTGATAGTTTGCTACAATTGAACATTGACTTTGCTTGTAGTAAAGTCAACTGTAGTGTAATTGAACCCGGTGGTGAATGCCAATTACCAGACACTATAATGAACCACGCGTCTGTTGCCATGAATCTTTACTACCAATCTTTTGGCAGAACAGATTTGAGCTGTTATTTTCAGTCGACCGGCATGGTTGTGATTGAAGATCCAAGTTCTGGAACTTGTGTCTATGAAGGGGTTGTCCTTGTGAGGGATGCTGTCCCTGCAAGTATCAAAATAAAGAAGGGGGAAAGTAAGGCCTTTTCTGCAACATCAATTGGACTTATGGTGGTGTGGTCGATCACTGGTTGTGGACTGGTGGCAGTGGCTGTATATGTTATGCTCTGGCTTCGTCAGAACCGCACACCAGTGGTGGGAGTTCCTGTGCACACATTGCCAGAGCCATTGTATTTGCGGCTGGAGCCATCAGCTCCCCCGCTTCCTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
X8

Protein Analysis

213

Amino Acids

22.77

Weight (kDa)

5.75

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 45 - 115 2.6e-18 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29735 AT4G09090 AT4G09462 AT4G09464 AT4G09465 AT4G09466 AT4G09467 AT5G53600 AT5G53610 AT5G63225 AT5G63230
fragaria_vesca FvH4_3g06020 FvH4_3g06020 FvH4_3g06020 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06030 FvH4_3g06040 FvH4_4g08590 FvH4_6g37860
malus_domestica MD10G1265100.v1.1
prunus_persica Prupe.4G076700_v2.0.a1 Prupe.4G076900_v2.0.a1 Prupe.4G084600_v2.0.a1 Prupe.4G130100_v2.0.a1 Prupe.6G294200_v2.0.a1
pyrus_communis pycom05g26640 pycom10g22110
rosa_chinensis RchiOBHm_Chr5g0013281 RchiOBHm_Chr5g0013291 RchiOBHm_Chr5g0013321 RchiOBHm_Chr5g0013341 RchiOBHm_Chr5g0013351 RchiOBHm_Chr5g0013361 RchiOBHm_Chr5g0013371 RchiOBHm_Chr5g0013381 RchiOBHm_Chr5g0013391
rosa_laevigata RLG00000032004 RLG00000032005 RLG00000032008 RLG00000032012 RLG00000032013 RLG00000032014 RLG00000032015 RLG00000032016
rosa_multiflora Rmu_co8200260.1_g000001 Rmu_co8451143.1_g000001 Rmu_sc0003458.1_g000006 Rmu_sc0003458.1_g000008 Rmu_sc0003458.1_g000009 Rmu_sc0003458.1_g000012 Rmu_sc0003458.1_g000014 Rmu_sc0010713.1_g000001
rosa_roxburghii Rroxscaffold_1G00063160 Rroxscaffold_1G00063170 Rroxscaffold_1G00063180 Rroxscaffold_1G00063190 Rroxscaffold_1G00063200 Rroxscaffold_1G00063210
rosa_rugosa Rorug05G0008700 Rorug05G0008700 Rorug05G0008800 Rorug05G0008800 Rorug05G0008900.1 Rorug05G0009000 Rorug05G0009000 Rorug05G0009000
rosa_samantha Rh5AG103700 Rh5AG103800 Rh5AG104000 Rh5AG104300 Rh5AG104400 Rh5BG100300 Rh5BG100400 Rh5BG100500 Rh5BG100600 Rh5BG100700 Rh5CG111500 Rh5CG111600 Rh5CG112000 Rh5CG112200 Rh5CG112300 Rh5CG112500 Rh5DG099200 Rh5DG099300
rosa_wichuraiana Rw5G009040 Rw5G009050 Rw5G009080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 95, 368
AccI GTMKAC 1 cut(s) 335
AccII CGCG 1 cut(s) 269
AciI CCGC 5 cut(s) 105, 159, 553, 607, 629
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 1 cut(s) 97
AfiI CCNNNNNNNGG 4 cut(s) 95, 368, 403, 566
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 3 cut(s) 181, 224, 356
AjnI CCWGG 2 cut(s) 88, 134
AleI CACNNNNGTG 1 cut(s) 560
AluBI AGCT 3 cut(s) 67, 321, 623
AluI AGCT 3 cut(s) 67, 321, 623
Alw21I GWGCWC 2 cut(s) 69, 582
Alw44I GTGCAC 1 cut(s) 578
AlwI GGATC 1 cut(s) 353
AlwNI CAGNNNCTG 1 cut(s) 521
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 3 cut(s) 39, 97, 450
ApaLI GTGCAC 1 cut(s) 578
AspLEI GCGC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 1 cut(s) 245
AvaI CYCGRG 1 cut(s) 62
BaeGI GKGCMC 1 cut(s) 582
BalI TGGCCA 1 cut(s) 99
BanII GRGCYC 1 cut(s) 69
Bbv12I GWGCWC 2 cut(s) 69, 582
BccI CCATC 1 cut(s) 625
BciT130I CCWGG 2 cut(s) 90, 136
BcnI CCSGG 1 cut(s) 228
BfmI CTRYAG 1 cut(s) 211
BisI GCNGC 3 cut(s) 105, 159, 608
BlsI GCNGC 3 cut(s) 106, 160, 609
Bme1390I CCNGG 3 cut(s) 90, 136, 228
BmeT110I CYCGRG 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 615
BmrFI CCNGG 3 cut(s) 90, 136, 228
BmsI GCATC 1 cut(s) 397
BpmI CTGGAG 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 228
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 4 cut(s) 95, 368, 403, 566
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 3 cut(s) 502, 513, 560
Bse3DI GCAATG 3 cut(s) 75, 120, 584
BseBI CCWGG 2 cut(s) 90, 136
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 4 cut(s) 95, 368, 403, 566
BseMI GCAATG 3 cut(s) 75, 120, 584
BseNI ACTGG 3 cut(s) 502, 513, 560
BseSI GKGCMC 1 cut(s) 582
Bsh1236I CGCG 1 cut(s) 269
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 41, 99, 452
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 2 cut(s) 69, 582
BsiHKCI CYCGRG 1 cut(s) 62
BsiSI CCGG 2 cut(s) 228, 339
BslFI GGGAC 1 cut(s) 398
BslI CCNNNNNNNGG 4 cut(s) 95, 368, 403, 566
BsmFI GGGAC 1 cut(s) 398
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 3 cut(s) 41, 99, 452
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 2 cut(s) 69, 582
Bsp143I GATC 3 cut(s) 82, 358, 492
BspACI CCGC 5 cut(s) 105, 159, 553, 607, 629
BspANI GGCC 3 cut(s) 41, 99, 452
BspFNI CGCG 1 cut(s) 269
BspLI GGNNCC 1 cut(s) 615
BspPI GGATC 1 cut(s) 353
BsrDI GCAATG 3 cut(s) 75, 120, 584
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 3 cut(s) 502, 513, 560
BssAI RCCGGY 1 cut(s) 338
BssMI GATC 3 cut(s) 82, 358, 492
Bst2UI CCWGG 2 cut(s) 90, 136
Bst4CI ACNGT 2 cut(s) 28, 212
BstAPI GCANNNNNTGC 2 cut(s) 122, 586
BstDEI CTNAG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 412
BstFNI CGCG 1 cut(s) 269
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 3 cut(s) 85, 361, 495
BstMBI GATC 3 cut(s) 82, 358, 492
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 8 cut(s) 47, 113, 122, 275, 416, 458, 586, 613
BstNI CCWGG 2 cut(s) 90, 136
BstSCI CCNGG 3 cut(s) 88, 134, 226
BstSFI CTRYAG 1 cut(s) 211
BstSLI GKGCMC 1 cut(s) 582
BstUI CGCG 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 358
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 3 cut(s) 41, 99, 452
BtsCI GGATG 1 cut(s) 412
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 4 cut(s) 24, 495, 522, 567
CaiI CAGNNNCTG 1 cut(s) 521
CfoI GCGC 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 338
CseI GACGC 1 cut(s) 258
CsiI ACCWGGT 1 cut(s) 134
CviAII CATG 5 cut(s) 95, 101, 112, 280, 343
DdeI CTNAG 1 cut(s) 126
DpnI GATC 3 cut(s) 84, 360, 494
DpnII GATC 3 cut(s) 82, 358, 492
EaeI YGGCCR 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 67
Eco147I AGGCCT 1 cut(s) 452
Eco24I GRGCYC 1 cut(s) 69
Eco53kI GAGCTC 1 cut(s) 67
Eco88I CYCGRG 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 67
EcoRII CCWGG 2 cut(s) 88, 134
EcoT38I GRGCYC 1 cut(s) 69
FaeI CATG 5 cut(s) 98, 104, 115, 283, 346
FaqI GGGAC 1 cut(s) 398
FatI CATG 5 cut(s) 94, 100, 111, 279, 342
FauI CCCGC 1 cut(s) 636
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 3 cut(s) 105, 159, 608
FokI GGATG 1 cut(s) 419
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 3 cut(s) 105, 159, 608
GlaI GCGC 1 cut(s) 73
GluI GCNGC 3 cut(s) 105, 159, 608
GsuI CTGGAG 1 cut(s) 632
HaeIII GGCC 3 cut(s) 41, 99, 452
HapII CCGG 2 cut(s) 228, 339
HgaI GACGC 1 cut(s) 258
HhaI GCGC 1 cut(s) 74
Hin1II CATG 5 cut(s) 98, 104, 115, 283, 346
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HincII GTYRAC 2 cut(s) 208, 336
HindII GTYRAC 2 cut(s) 208, 336
HinfI GANTC 1 cut(s) 283
HpaII CCGG 2 cut(s) 228, 339
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 4 cut(s) 208, 336, 506, 580
Hpy188I TCNGA 1 cut(s) 549
Hpy188III TCNNGA 2 cut(s) 55, 369
Hpy8I GTNNAC 4 cut(s) 208, 336, 506, 580
HpyAV CCTTC 4 cut(s) 29, 52, 380, 430
HpyCH4III ACNGT 2 cut(s) 28, 212
HpyCH4V TGCA 3 cut(s) 419, 461, 580
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 113, 122, 275, 416, 458, 586, 613
HpyF3I CTNAG 1 cut(s) 126
Hsp92II CATG 5 cut(s) 98, 104, 115, 283, 346
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 3 cut(s) 82, 358, 492
LmnI GCTCC 2 cut(s) 613, 628
LweI GCATC 1 cut(s) 397
MabI ACCWGGT 1 cut(s) 134
MalI GATC 3 cut(s) 84, 360, 494
MboI GATC 3 cut(s) 82, 358, 492
MboII GAAGA 2 cut(s) 77, 368
MfeI CAATTG 2 cut(s) 176, 468
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 2 cut(s) 69, 582
MlsI TGGCCA 1 cut(s) 99
MluCI AATT 4 cut(s) 176, 219, 242, 468
MluI ACGCGT 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 99
MnlI CCTC 3 cut(s) 56, 71, 396
Mox20I TGGCCA 1 cut(s) 99
MscI TGGCCA 1 cut(s) 99
MslI CAYNNNNRTG 4 cut(s) 99, 257, 347, 560
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 2 cut(s) 228, 339
MspR9I CCNGG 3 cut(s) 90, 136, 228
MunI CAATTG 2 cut(s) 176, 468
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 2 cut(s) 90, 136
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 8 cut(s) 47, 113, 122, 275, 416, 458, 586, 613
NciI CCSGG 1 cut(s) 228
NdeII GATC 3 cut(s) 82, 358, 492
NlaIII CATG 5 cut(s) 98, 104, 115, 283, 346
NlaIV GGNNCC 1 cut(s) 615
OliI CACNNNNGTG 1 cut(s) 560
PaeR7I CTCGAG 1 cut(s) 62
PceI AGGCCT 1 cut(s) 452
PctI GAATGC 1 cut(s) 242
PfeI GAWTC 1 cut(s) 283
PflMI CCANNNNNTGG 2 cut(s) 95, 368
PfoI TCCNGGA 1 cut(s) 88
PkrI GCNGC 3 cut(s) 106, 160, 609
Psp124BI GAGCTC 1 cut(s) 69
Psp6I CCWGG 2 cut(s) 88, 134
PspGI CCWGG 2 cut(s) 88, 134
PspN4I GGNNCC 1 cut(s) 615
PspXI VCTCGAGB 1 cut(s) 62
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 358
RseI CAYNNNNRTG 4 cut(s) 99, 257, 347, 560
SacI GAGCTC 1 cut(s) 69
SalI GTCGAC 1 cut(s) 334
SatI GCNGC 3 cut(s) 105, 159, 608
Sau3AI GATC 3 cut(s) 82, 358, 492
ScrFI CCNGG 3 cut(s) 90, 136, 228
SduI GDGCHC 2 cut(s) 69, 582
SetI ASST 5 cut(s) 63, 69, 137, 323, 625
SexAI ACCWGGT 1 cut(s) 134
SfaNI GCATC 1 cut(s) 397
SfcI CTRYAG 1 cut(s) 211
Sfr274I CTCGAG 1 cut(s) 62
SlaI CTCGAG 1 cut(s) 62
SmiMI CAYNNNNRTG 4 cut(s) 99, 257, 347, 560
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 4 cut(s) 176, 219, 242, 468
SseBI AGGCCT 1 cut(s) 452
SsiI CCGC 5 cut(s) 105, 159, 553, 607, 629
SstI GAGCTC 1 cut(s) 69
StuI AGGCCT 1 cut(s) 452
StyD4I CCNGG 3 cut(s) 88, 134, 226
TaaI ACNGT 2 cut(s) 28, 212
TaqI TCGA 4 cut(s) 56, 63, 335, 491
TasI AATT 4 cut(s) 176, 219, 242, 468
TauI GCSGC 3 cut(s) 107, 161, 610
TfiI GAWTC 1 cut(s) 283
TscAI CASTG 4 cut(s) 31, 502, 522, 567
TspDTI ATGAA 4 cut(s) 100, 275, 296, 399
TspRI CASTG 4 cut(s) 31, 502, 522, 567
Van91I CCANNNNNTGG 2 cut(s) 95, 368
VneI GTGCAC 1 cut(s) 578
XhoI CTCGAG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.