FvH4_1g20711

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000492
Physical Location & Seq
Forward (+)
12863720 .. 12864118
399 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20711.t1

Sequence Viewer

Length: 399 bp
ATGAGTAAAGCCTACGATAGGGTTGAGTGGGATTTTTTGGAGAGGGTTATGCAGAATATGGAGTTTAACAATCAGTGGATCAAGTGGATCATGGACTGTGTCTCTACGCTATCTTATTCTTTTATCTTAAACGGAGAACCAAGGGGCAGATTGGTACCCTCGAGGGGTATAAGGCAGAGGGATTCAATCTCACCCTATCTATTTTTGTTATGTGCTGAAGGTTTGTCGAGAATGTTGACTTATGCAGAGAGTCGGGGTTTTATTCATGGGGTGGTTGTAGCGCCAGGAGCTCCCTCCATAAACCACCTATTTTTCATGAATGATTCTTCGTGTTCTGAGGAAGAGGAATGTACTAGGTTGAAGAATATCCTCCAGGCATATGAAGAAGCAAGTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

133

Amino Acids

15.2

Weight (kDa)

5.17

Isoelectric Point (pI)

57.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 2 - 128 2.7e-09 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000492)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01250
fragaria_vesca FvH4_1g20711 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g28252 FvH4_3g31041 FvH4_4g00611 FvH4_5g15581 FvH4_5g21911 FvH4_5g35851 FvH4_6g24331 FvH4_6g34741
malus_domestica MD07G1291800.v1.1 MD09G1222500.v1.1
prunus_persica Prupe.1G162400_v2.0.a1 Prupe.1G210400_v2.0.a1 Prupe.6G308200_v2.0.a1
pyrus_communis pycom05g02580 pycom12g09210 pycom12g15310 pycom16g09140
rosa_chinensis RchiOBHm_Chr3g0484711 RchiOBHm_Chr4g0393411 RchiOBHm_Chr4g0397521 RchiOBHm_Chr4g0404571 RchiOBHm_Chr4g0418351 RchiOBHm_Chr5g0060311 RchiOBHm_Chr6g0301391 RchiOBHm_Chr7g0229871
rosa_laevigata RLG00000007836 RLG00000025145
rosa_multiflora Rmu_co8327795.1_g000001 Rmu_sc0000469.1_g000005 Rmu_sc0000498.1_g000050 Rmu_sc0000558.1_g000007 Rmu_sc0000693.1_g000082 Rmu_sc0000711.1_g000011 Rmu_sc0000814.1_g000039 Rmu_sc0001366.1_g000005 Rmu_sc0002205.1_g000004 Rmu_sc0002283.1_g000086 Rmu_sc0002406.1_g000011 Rmu_sc0002735.1_g000022 Rmu_sc0003545.1_g000004 Rmu_sc0004406.1_g000009 Rmu_sc0006301.1_g000009 Rmu_sc0006754.1_g000004 Rmu_sc0007324.1_g000010 Rmu_sc0008199.1_g000002 Rmu_sc0008563.1_g000009 Rmu_sc0009777.1_g000007 Rmu_sc0010560.1_g000011 Rmu_sc0011095.1_g000004 Rmu_sc0012101.1_g000004 Rmu_sc0014150.1_g000009 Rmu_sc0020270.1_g000004 Rmu_sc0021327.1_g000001 Rmu_sc0031326.1_g000003 Rmu_ssc0000116.1_g000050 Rmu_ssc0000368.1_g000056
rosa_roxburghii Rroxscaffold_2G00110080 Rroxscaffold_2G00141700 Rroxscaffold_2G00142400
rosa_rugosa Rorug01G0104400 Rorug01G0204500 Rorug02G0225600 Rorug02G0262000.1 Rorug02G0526700 Rorug03G0082000 Rorug03G0102500.1 Rorug04G0106400 Rorug04G0200200 Rorug05G0021900.1 Rorug05G0240000 Rorug05G0326500 Rorug05G0529200 Rorug07G0136100
rosa_samantha Rh1AG352100 Rh6DG494100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 160
Acc65I GGTACC 1 cut(s) 154
AccB1I GGYRCC 1 cut(s) 154
AclWI GGATC 2 cut(s) 86, 95
AcuI CTGAAG 1 cut(s) 237
AfaI GTAC 2 cut(s) 156, 352
AfiI CCNNNNNNNGG 2 cut(s) 18, 164
AgsI TTSAA 2 cut(s) 186, 361
AjnI CCWGG 2 cut(s) 283, 372
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
Alw21I GWGCWC 1 cut(s) 292
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 2 cut(s) 86, 95
Ama87I CYCGRG 1 cut(s) 160
Asp718I GGTACC 1 cut(s) 154
AspLEI GCGC 1 cut(s) 283
AsuHPI GGTGA 1 cut(s) 183
AvaI CYCGRG 1 cut(s) 160
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 292
BciT130I CCWGG 2 cut(s) 285, 374
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 1 cut(s) 354
BfoI RGCGCY 1 cut(s) 284
Bme1390I CCNGG 2 cut(s) 285, 374
BmeT110I CYCGRG 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 156
BmrFI CCNGG 2 cut(s) 285, 374
BpmI CTGGAG 1 cut(s) 356
BsaJI CCNNGG 1 cut(s) 140
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 164
BseBI CCWGG 2 cut(s) 285, 374
BseDI CCNNGG 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 18, 164
BseMII CTCAG 1 cut(s) 327
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 292
BsiHKCI CYCGRG 1 cut(s) 160
BslI CCNNNNNNNGG 2 cut(s) 18, 164
BsmAI GTCTC 1 cut(s) 106
BsoBI CYCGRG 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 292
Bsp143I GATC 2 cut(s) 78, 87
BspCNI CTCAG 1 cut(s) 328
BspHI TCATGA 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 2 cut(s) 86, 95
BspT107I GGYRCC 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 2 cut(s) 78, 87
BssT1I CCWWGG 1 cut(s) 140
Bst2UI CCWGG 2 cut(s) 285, 374
Bst4CI ACNGT 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 336
BstDEI CTNAG 1 cut(s) 336
BstENI CCTNNNNNAGG 1 cut(s) 16
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 2 cut(s) 81, 90
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 2 cut(s) 78, 87
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 2 cut(s) 285, 374
BstSCI CCNGG 2 cut(s) 283, 372
BtsIMutI CAGTG 1 cut(s) 80
CciI TCATGA 1 cut(s) 315
CfoI GCGC 1 cut(s) 283
Csp6I GTAC 2 cut(s) 155, 351
CviAII CATG 3 cut(s) 91, 266, 316
CviJI RGCY 2 cut(s) 11, 290
CviKI_1 RGCY 2 cut(s) 11, 290
CviQI GTAC 2 cut(s) 155, 351
DdeI CTNAG 1 cut(s) 336
DpnI GATC 2 cut(s) 80, 89
DpnII GATC 2 cut(s) 78, 87
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Ecl136II GAGCTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 292
Eco53kI GAGCTC 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 290
EcoNI CCTNNNNNAGG 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 283, 372
EcoT14I CCWWGG 1 cut(s) 140
EcoT38I GRGCYC 1 cut(s) 292
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 3 cut(s) 94, 269, 319
FatI CATG 3 cut(s) 90, 265, 315
FauNDI CATATG 1 cut(s) 379
FriOI GRGCYC 1 cut(s) 292
FspBI CTAG 1 cut(s) 354
GlaI GCGC 1 cut(s) 282
GsuI CTGGAG 1 cut(s) 356
HaeII RGCGCY 1 cut(s) 284
HhaI GCGC 1 cut(s) 283
Hin1II CATG 3 cut(s) 94, 269, 319
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 237
HindII GTYRAC 1 cut(s) 237
HinfI GANTC 3 cut(s) 182, 250, 323
HphI GGTGA 1 cut(s) 183
Hpy166II GTNNAC 1 cut(s) 237
Hpy188I TCNGA 1 cut(s) 337
Hpy188III TCNNGA 2 cut(s) 228, 316
Hpy8I GTNNAC 1 cut(s) 237
HpyAV CCTTC 1 cut(s) 212
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4V TGCA 2 cut(s) 52, 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 1 cut(s) 336
Hsp92II CATG 3 cut(s) 94, 269, 319
HspAI GCGC 1 cut(s) 281
KpnI GGTACC 1 cut(s) 158
Kzo9I GATC 2 cut(s) 78, 87
LmnI GCTCC 2 cut(s) 287, 295
LpnPI CCDG 4 cut(s) 270, 297, 359, 386
MaeI CTAG 1 cut(s) 354
MalI GATC 2 cut(s) 80, 89
MboI GATC 2 cut(s) 78, 87
MboII GAAGA 4 cut(s) 318, 353, 373, 395
MhlI GDGCHC 1 cut(s) 292
MlyI GAGTC 1 cut(s) 259
MnlI CCTC 8 cut(s) 36, 156, 169, 171, 304, 331, 337, 380
MseI TTAA 2 cut(s) 66, 128
MspR9I CCNGG 2 cut(s) 285, 374
MvaI CCWGG 2 cut(s) 285, 374
MwoI GCNNNNNNNGC 1 cut(s) 287
NdeI CATATG 1 cut(s) 379
NdeII GATC 2 cut(s) 78, 87
NlaIII CATG 3 cut(s) 94, 269, 319
NlaIV GGNNCC 1 cut(s) 156
PaeR7I CTCGAG 1 cut(s) 160
PagI TCATGA 1 cut(s) 315
PfeI GAWTC 2 cut(s) 182, 323
PflFI GACNNNGTC 1 cut(s) 98
PleI GAGTC 1 cut(s) 258
PpsI GAGTC 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 292
Psp6I CCWGG 2 cut(s) 283, 372
PspGI CCWGG 2 cut(s) 283, 372
PspN4I GGNNCC 1 cut(s) 156
PspXI VCTCGAGB 1 cut(s) 160
PsyI GACNNNGTC 1 cut(s) 98
RsaI GTAC 2 cut(s) 156, 352
RsaNI GTAC 2 cut(s) 155, 351
SacI GAGCTC 1 cut(s) 292
SaqAI TTAA 2 cut(s) 66, 128
Sau3AI GATC 2 cut(s) 78, 87
SchI GAGTC 1 cut(s) 259
ScrFI CCNGG 2 cut(s) 285, 374
SduI GDGCHC 1 cut(s) 292
SetI ASST 4 cut(s) 223, 292, 309, 359
Sfr274I CTCGAG 1 cut(s) 160
SlaI CTCGAG 1 cut(s) 160
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
SspMI CTAG 1 cut(s) 354
SstI GAGCTC 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 283, 372
StyI CCWWGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 98
TaqI TCGA 2 cut(s) 161, 227
TatI WGTACW 1 cut(s) 350
TfiI GAWTC 2 cut(s) 182, 323
Tru1I TTAA 2 cut(s) 66, 128
Tru9I TTAA 2 cut(s) 66, 128
TscAI CASTG 1 cut(s) 80
TspDTI ATGAA 4 cut(s) 254, 304, 332, 396
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 1 cut(s) 80
Tth111I GACNNNGTC 1 cut(s) 98
XagI CCTNNNNNAGG 1 cut(s) 16
XhoI CTCGAG 1 cut(s) 160
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.