FvH4_3g28252

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000492
Physical Location & Seq
Forward (+)
21238608 .. 21239465
858 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28252.t1

Sequence Viewer

Length: 657 bp
ATGATGCTTTCATTTGGGGCCCCCTGCCCGAATGGTGAGTTCACTATAAAATCAACTACTTGGCTTCAATCTTCAGATCTTGATCCTCATGATCAATCTCCTCTTTTAAAGAAGTGCAATGACATCCATAATGCTGATAACCTGGTTCAAAGGTTGAATGATATTTTTGTTAATGCTCCTTTTGATGCTACTAAGTTTGAGAGAATTATAACCCTTTGCTGGCAGATTTGGCATAAGCGCAACAACCTAATCTTTAAAAATGAGGTTTTCTCTACCTCTGTTGTTGTTGTTGTTGTTGCTGATGCGTTTCAGAATCGTATTCATCTTCACCAAGAGATGGCTTCCCAGTCCCCCTTGGCTCCCTCTTCCATCAGATGGACTCCTCCTCCTCAAAATATAGTCAAAGTGAATTTTGATGGATCGGTGCTTCAACATGATTCTAGTGCTGCTGCAGATTTTATTTTTCGAGACTGCTCGGATTGCCCTTTGTTTGCTTCTGCTCGAAAGTTGGGGAAAGCCATTGTTCCCATTGCAGAAGCTATGGCCCTTAGGGACAGCCTTTTAAAAGCCAACGAGTTAAACTACTCTCATATTATGATTGAAGGGGACTCTGCCCTCATAATTAATTGTGTTACTGGGCGTTTCAAGTGCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

24.37

Weight (kDa)

6.36

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 137 - 215 6.5e-12 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000492)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01250
fragaria_vesca FvH4_1g20711 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g28252 FvH4_3g31041 FvH4_4g00611 FvH4_5g15581 FvH4_5g21911 FvH4_5g35851 FvH4_6g24331 FvH4_6g34741
malus_domestica MD07G1291800.v1.1 MD09G1222500.v1.1
prunus_persica Prupe.1G162400_v2.0.a1 Prupe.1G210400_v2.0.a1 Prupe.6G308200_v2.0.a1
pyrus_communis pycom05g02580 pycom12g09210 pycom12g15310 pycom16g09140
rosa_chinensis RchiOBHm_Chr3g0484711 RchiOBHm_Chr4g0393411 RchiOBHm_Chr4g0397521 RchiOBHm_Chr4g0404571 RchiOBHm_Chr4g0418351 RchiOBHm_Chr5g0060311 RchiOBHm_Chr6g0301391 RchiOBHm_Chr7g0229871
rosa_laevigata RLG00000007836 RLG00000025145
rosa_multiflora Rmu_co8327795.1_g000001 Rmu_sc0000469.1_g000005 Rmu_sc0000498.1_g000050 Rmu_sc0000558.1_g000007 Rmu_sc0000693.1_g000082 Rmu_sc0000711.1_g000011 Rmu_sc0000814.1_g000039 Rmu_sc0001366.1_g000005 Rmu_sc0002205.1_g000004 Rmu_sc0002283.1_g000086 Rmu_sc0002406.1_g000011 Rmu_sc0002735.1_g000022 Rmu_sc0003545.1_g000004 Rmu_sc0004406.1_g000009 Rmu_sc0006301.1_g000009 Rmu_sc0006754.1_g000004 Rmu_sc0007324.1_g000010 Rmu_sc0008199.1_g000002 Rmu_sc0008563.1_g000009 Rmu_sc0009777.1_g000007 Rmu_sc0010560.1_g000011 Rmu_sc0011095.1_g000004 Rmu_sc0012101.1_g000004 Rmu_sc0014150.1_g000009 Rmu_sc0020270.1_g000004 Rmu_sc0021327.1_g000001 Rmu_sc0031326.1_g000003 Rmu_ssc0000116.1_g000050 Rmu_ssc0000368.1_g000056
rosa_roxburghii Rroxscaffold_2G00110080 Rroxscaffold_2G00141700 Rroxscaffold_2G00142400
rosa_rugosa Rorug01G0104400 Rorug01G0204500 Rorug02G0225600 Rorug02G0262000.1 Rorug02G0526700 Rorug03G0082000 Rorug03G0102500.1 Rorug04G0106400 Rorug04G0200200 Rorug05G0021900.1 Rorug05G0240000 Rorug05G0326500 Rorug05G0529200 Rorug07G0136100
rosa_samantha Rh1AG352100 Rh6DG494100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 209
AccB7I CCANNNNNTGG 2 cut(s) 337, 375
AclWI GGATC 2 cut(s) 77, 427
AcsI RAATTY 1 cut(s) 409
AcuI CTGAAG 1 cut(s) 57
AfiI CCNNNNNNNGG 3 cut(s) 219, 337, 375
AgsI TTSAA 6 cut(s) 68, 149, 157, 431, 602, 646
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 1 cut(s) 539
AluI AGCT 1 cut(s) 539
Alw26I GTCTC 1 cut(s) 462
AlwI GGATC 2 cut(s) 77, 427
AoxI GGCC 2 cut(s) 18, 543
ApaI GGGCCC 1 cut(s) 22
ApeKI GCWGC 2 cut(s) 446, 449
ApoI RAATTY 1 cut(s) 409
AseI ATTAAT 1 cut(s) 624
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 3 cut(s) 18, 19, 544
AsuHPI GGTGA 2 cut(s) 47, 320
AxyI CCTNAGG 1 cut(s) 548
BaeGI GKGCMC 2 cut(s) 22, 653
BanII GRGCYC 1 cut(s) 22
BbvI GCAGC 2 cut(s) 433, 436
BccI CCATC 4 cut(s) 331, 369, 377, 410
BciT130I CCWGG 1 cut(s) 143
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 462
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 1 cut(s) 450
BglII AGATCT 1 cut(s) 76
BisI GCNGC 2 cut(s) 447, 450
BlsI GCNGC 2 cut(s) 448, 451
Bme1390I CCNGG 1 cut(s) 143
BmgT120I GGNCC 3 cut(s) 18, 19, 544
BmiI GGNNCC 4 cut(s) 19, 20, 21, 360
BmrFI CCNGG 1 cut(s) 143
BmrI ACTGGG 2 cut(s) 340, 645
BmsI GCATC 2 cut(s) 175, 292
BmuI ACTGGG 2 cut(s) 340, 645
BplI GAGNNNNNCTC 2 cut(s) 254, 286
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 354
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 3 cut(s) 219, 337, 375
Bse1I ACTGG 2 cut(s) 346, 640
Bse21I CCTNAGG 1 cut(s) 548
Bse3DI GCAATG 2 cut(s) 124, 528
Bse8I GATNNNNATC 1 cut(s) 81
BseBI CCWGG 1 cut(s) 143
BseDI CCNNGG 1 cut(s) 354
BseGI GGATG 1 cut(s) 123
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 3 cut(s) 219, 337, 375
BseMI GCAATG 2 cut(s) 124, 528
BseNI ACTGG 2 cut(s) 346, 640
BseRI GAGGAG 4 cut(s) 90, 372, 375, 378
BseSI GKGCMC 2 cut(s) 22, 653
BseXI GCAGC 2 cut(s) 433, 436
BshFI GGCC 2 cut(s) 20, 545
BslFI GGGAC 3 cut(s) 334, 566, 620
BslI CCNNNNNNNGG 3 cut(s) 219, 337, 375
BsmAI GTCTC 1 cut(s) 462
BsmFI GGGAC 3 cut(s) 334, 566, 620
BsnI GGCC 2 cut(s) 20, 545
Bsp120I GGGCCC 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 22, 653
Bsp143I GATC 4 cut(s) 76, 82, 91, 419
BspANI GGCC 2 cut(s) 20, 545
BspHI TCATGA 1 cut(s) 88
BspLI GGNNCC 4 cut(s) 19, 20, 21, 360
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 2 cut(s) 77, 427
BsrDI GCAATG 2 cut(s) 124, 528
BsrI ACTGG 2 cut(s) 346, 640
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 4 cut(s) 76, 82, 91, 419
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 143
Bst6I CTCTTC 1 cut(s) 370
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 2 cut(s) 192, 548
BstF5I GGATG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 4 cut(s) 79, 85, 94, 422
BstMAI GTCTC 1 cut(s) 462
BstMBI GATC 4 cut(s) 76, 82, 91, 419
BstMWI GCNNNNNNNGC 2 cut(s) 229, 480
BstNI CCWGG 1 cut(s) 143
BstSCI CCNGG 1 cut(s) 141
BstSFI CTRYAG 1 cut(s) 450
BstSLI GKGCMC 2 cut(s) 22, 653
BstV1I GCAGC 2 cut(s) 433, 436
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
Bsu36I CCTNAGG 1 cut(s) 548
BsuRI GGCC 2 cut(s) 20, 545
BtsCI GGATG 1 cut(s) 123
Cac8I GCNNGC 1 cut(s) 221
CciI TCATGA 1 cut(s) 88
CfoI GCGC 1 cut(s) 240
Cfr13I GGNCC 3 cut(s) 18, 19, 544
CsiI ACCWGGT 1 cut(s) 141
CviAII CATG 2 cut(s) 89, 434
CviJI RGCY 9 cut(s) 20, 64, 341, 359, 518, 539, 545, 558, 569
CviKI_1 RGCY 9 cut(s) 20, 64, 341, 359, 518, 539, 545, 558, 569
DdeI CTNAG 2 cut(s) 192, 548
DpnI GATC 4 cut(s) 78, 84, 93, 421
DpnII GATC 4 cut(s) 76, 82, 91, 419
DraI TTTAAA 3 cut(s) 108, 256, 564
Eam1104I CTCTTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 370
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 548
EcoO109I RGGNCCY 2 cut(s) 18, 19
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 354
EcoT38I GRGCYC 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 2 cut(s) 92, 437
FaqI GGGAC 3 cut(s) 334, 566, 620
FatI CATG 2 cut(s) 88, 433
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 2 cut(s) 447, 450
FokI GGATG 1 cut(s) 110
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 2 cut(s) 447, 450
FspBI CTAG 1 cut(s) 441
GlaI GCGC 1 cut(s) 239
GluI GCNGC 2 cut(s) 447, 450
HaeIII GGCC 2 cut(s) 20, 545
HhaI GCGC 1 cut(s) 240
Hin1II CATG 2 cut(s) 92, 437
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HinfI GANTC 4 cut(s) 313, 379, 437, 608
HphI GGTGA 2 cut(s) 47, 320
Hpy166II GTNNAC 1 cut(s) 42
Hpy188I TCNGA 4 cut(s) 76, 312, 374, 478
Hpy188III TCNNGA 3 cut(s) 80, 89, 467
Hpy8I GTNNAC 1 cut(s) 42
HpyAV CCTTC 1 cut(s) 596
HpyCH4V TGCA 3 cut(s) 117, 452, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 480
HpyF3I CTNAG 2 cut(s) 192, 548
Hsp92II CATG 2 cut(s) 92, 437
HspAI GCGC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 4 cut(s) 76, 82, 91, 419
LmnI GCTCC 2 cut(s) 181, 364
LpnPI CCDG 6 cut(s) 37, 128, 155, 205, 359, 621
Lsp1109I GCAGC 2 cut(s) 433, 436
LweI GCATC 2 cut(s) 175, 292
MabI ACCWGGT 1 cut(s) 141
MaeI CTAG 1 cut(s) 441
MaeIII GTNAC 1 cut(s) 631
MalI GATC 4 cut(s) 78, 84, 93, 421
MboI GATC 4 cut(s) 76, 82, 91, 419
MboII GAAGA 3 cut(s) 63, 317, 357
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 2 cut(s) 22, 653
MluCI AATT 4 cut(s) 204, 409, 621, 625
MlyI GAGTC 2 cut(s) 373, 602
MnlI CCTC 9 cut(s) 96, 111, 256, 286, 373, 393, 396, 399, 626
MseI TTAA 6 cut(s) 107, 171, 255, 563, 578, 624
MspR9I CCNGG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 143
MwoI GCNNNNNNNGC 2 cut(s) 229, 480
NdeII GATC 4 cut(s) 76, 82, 91, 419
NlaIII CATG 2 cut(s) 92, 437
NlaIV GGNNCC 4 cut(s) 19, 20, 21, 360
PagI TCATGA 1 cut(s) 88
PfeI GAWTC 2 cut(s) 313, 437
PflMI CCANNNNNTGG 2 cut(s) 337, 375
PkrI GCNGC 2 cut(s) 448, 451
PleI GAGTC 2 cut(s) 373, 602
PpsI GAGTC 2 cut(s) 373, 602
PshBI ATTAAT 1 cut(s) 624
PsiI TTATAA 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspN4I GGNNCC 4 cut(s) 19, 20, 21, 360
PspOMI GGGCCC 1 cut(s) 18
PspPI GGNCC 3 cut(s) 18, 19, 544
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 76
SaqAI TTAA 6 cut(s) 107, 171, 255, 563, 578, 624
SatI GCNGC 2 cut(s) 447, 450
Sau3AI GATC 4 cut(s) 76, 82, 91, 419
Sau96I GGNCC 3 cut(s) 18, 19, 544
SchI GAGTC 2 cut(s) 373, 602
ScrFI CCNGG 1 cut(s) 143
SduI GDGCHC 2 cut(s) 22, 653
SetI ASST 6 cut(s) 144, 155, 249, 267, 278, 541
SexAI ACCWGGT 1 cut(s) 141
SfaNI GCATC 2 cut(s) 175, 292
SfcI CTRYAG 1 cut(s) 450
Sse9I AATT 4 cut(s) 204, 409, 621, 625
SspMI CTAG 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 141
StyI CCWWGG 1 cut(s) 354
TaqI TCGA 2 cut(s) 466, 502
TasI AATT 4 cut(s) 204, 409, 621, 625
TfiI GAWTC 2 cut(s) 313, 437
Tru1I TTAA 6 cut(s) 107, 171, 255, 563, 578, 624
Tru9I TTAA 6 cut(s) 107, 171, 255, 563, 578, 624
TseI GCWGC 2 cut(s) 446, 449
TspDTI ATGAA 1 cut(s) 311
Van91I CCANNNNNTGG 2 cut(s) 337, 375
VspI ATTAAT 1 cut(s) 624
XapI RAATTY 1 cut(s) 409
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.