Rorug05G0529200

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000492
Physical Location & Seq
Forward (+)
71846877 .. 71850208
3332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0529200.1

Sequence Viewer

Length: 1995 bp
ATGGACTTAGACGTCGACGTTTCGCGGTGGGTCCTCGAGCTCCTCCTCCGCGACCGCGACAAGGAGAGCGTCGCCAAGCGAGTCCTCGCCGCCGTGCCGCTCTCGAACCAGGACTGGCGGTTGAAGAAGACCGTCCTTCTCAGAACGATCGAGTCCGAAGTCTTGGACGAGGCTTCGGTCACCGAAGCTATACTAGAGTCCTTGGAGTCGATCGAGGCTTTGGACCGCGGCCAAGGGATTCCGGTCACGGACTCCATGAGAGCGGCGTACTGCGCCGTGGCGACGGAGTGCACGGTGAAGTTTTTGGTTTGCTTTGGAGGGAAGCCGAGCGGGAAGTACATGGAGGCGGTTGATCGGATTTGGAGAGGTAGGGTTAGGGTTTTGGAGGAGTCTGAGGGTTGCGAGCTGGTCTCGGGTGAGTTGAAGGAGAGAAGGGATGAGGTGGAGGCTGGGATTTGGGATGTGAAGGTTTCGAAGAAGCTGGCGAGCATGAATAGTAGAAATGATGCGCTGAGATTGGTTGCGGCGTATGTGAAGGAAGCTTTTGCGATGCTGGGTCCTCCGTTTATTGCTTGGGCTGTTAGGTTTAATATGCAGAAGGGAAGTTTAGGGTTGGAAGGTGATGAGGTTGCGGTGGAGAAGGATGGTAATGTGGGTGATGGGTGTGAAGTGCAGGTTCGAACGGCAAATGGGGCTGATTTGGAGGTGCGAAGGGCGAATGGGGTTGAGTTGGGTGATGGCTCCAAATTGGAGGAGAGTGGGGCTAATGTGCATGATGGGTCTGAATTGGAAGCGCAGAGAGGGGCAAATGTCAATTCTGGGTCTGAACAGGAGGCGCAAAGGGCGAATGTGGTTACTGTTAATTCTGGGTCTGAACAAGAGGCACAAAGGGCTAATGGGGCTAATGTCAATTCTGGGTCTGAATTGGAGGCACAAAGAGTGAATGGGGCAAATGATGTTAGTGATAGGTCTGAACAGGAGGCGCAAAGGGTGAATGGGGCAAATGATGTTAGTGATAGGTCTGAACAGGAGGCACAAAGGGCGAATGGGGCTCATGTTAGTGACGCCTCTGAACAGGAGGCGCATGATACTAATGTTGTGGGGGCAGGGAGATCACAACGTAATGGACACCGTGGTGTCCTCAGCTTAGAACCTGCAACTAAAGAAAAAGAAACTCCAAGGAGCAGGGCACTTAGACGTAGGCATGGACCGGTTAAGATTAGAGATGCTGAAGATGTGGGTACGGAAGCATCAGCTGGCATATATAACTCAGTGTCTAGTGCTGAAGTTAGAAAAGTGCAAGAAGAACTTAAGTCCAGTGTTTTGGCTCTACAAGCTGTAGTGACAGACCCACTTCCCTATGCTTTGCAAGTGTCGGAAATTGTAAGATCTGAGTTAGGAATGAGACCTGTGGATCATGTAAGTGGAGAAAATGGAGGTGCAGCAAATCCATCTGTTGAAAAGAACTCTGAGCCTGATCAATCTAATAATGATAATCATGGAAACCAGACCAGTCATCAGAATGATGTTCCCCAACCAAGTGGAAAAGAAAATGATGCAACAAATCTGTCTGTCAACAAGGGTAAGGGCACAGAGTATGTTCGAACGGGTGATGTCAATCCTGGCAATCCGTCTTGCAGTTATCAGAATAATGTTTCCCGAGCTGGCTTGATGGAACGTAATAGTACTGCACATACTCTTGAGTGGGATGACTCGCTAGGTATGTCTCCTGAACGAACAAAGAGTAAGGGGAGGCTTCACCTACCTAGTCCAAAAAGGAACGCTGTTTCTCCACTGAAGAAGTATGAGGATAAAAGATTTTCTAAGAGGAGAAAAGTTAAGAGATGGAGCTTACATGAAGAAGATACGTTAAGGACTGGTGTGCAGAAGTATGGCAGAGGCAATTGGAAGTTTATCTTAGATACATACCGAGATGTATTTGAAGAGAGAACTGAGGTCGATCTGAAGGACAAGTGGAGAAACATGACGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

664

Amino Acids

72.96

Weight (kDa)

5.5

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 614 - 662 7.3e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000492)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01250
fragaria_vesca FvH4_1g20711 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g28252 FvH4_3g31041 FvH4_4g00611 FvH4_5g15581 FvH4_5g21911 FvH4_5g35851 FvH4_6g24331 FvH4_6g34741
malus_domestica MD07G1291800.v1.1 MD09G1222500.v1.1
prunus_persica Prupe.1G162400_v2.0.a1 Prupe.1G210400_v2.0.a1 Prupe.6G308200_v2.0.a1
pyrus_communis pycom05g02580 pycom12g09210 pycom12g15310 pycom16g09140
rosa_chinensis RchiOBHm_Chr3g0484711 RchiOBHm_Chr4g0393411 RchiOBHm_Chr4g0397521 RchiOBHm_Chr4g0404571 RchiOBHm_Chr4g0418351 RchiOBHm_Chr5g0060311 RchiOBHm_Chr6g0301391 RchiOBHm_Chr7g0229871
rosa_laevigata RLG00000007836 RLG00000025145
rosa_multiflora Rmu_co8327795.1_g000001 Rmu_sc0000469.1_g000005 Rmu_sc0000498.1_g000050 Rmu_sc0000558.1_g000007 Rmu_sc0000693.1_g000082 Rmu_sc0000711.1_g000011 Rmu_sc0000814.1_g000039 Rmu_sc0001366.1_g000005 Rmu_sc0002205.1_g000004 Rmu_sc0002283.1_g000086 Rmu_sc0002406.1_g000011 Rmu_sc0002735.1_g000022 Rmu_sc0003545.1_g000004 Rmu_sc0004406.1_g000009 Rmu_sc0006301.1_g000009 Rmu_sc0006754.1_g000004 Rmu_sc0007324.1_g000010 Rmu_sc0008199.1_g000002 Rmu_sc0008563.1_g000009 Rmu_sc0009777.1_g000007 Rmu_sc0010560.1_g000011 Rmu_sc0011095.1_g000004 Rmu_sc0012101.1_g000004 Rmu_sc0014150.1_g000009 Rmu_sc0020270.1_g000004 Rmu_sc0021327.1_g000001 Rmu_sc0031326.1_g000003 Rmu_ssc0000116.1_g000050 Rmu_ssc0000368.1_g000056
rosa_roxburghii Rroxscaffold_2G00110080 Rroxscaffold_2G00141700 Rroxscaffold_2G00142400
rosa_rugosa Rorug01G0104400 Rorug01G0204500 Rorug02G0225600 Rorug02G0262000.1 Rorug02G0526700 Rorug03G0082000 Rorug03G0102500.1 Rorug04G0106400 Rorug04G0200200 Rorug05G0021900.1 Rorug05G0240000 Rorug05G0326500 Rorug05G0529200 Rorug07G0136100
rosa_samantha Rh1AG352100 Rh6DG494100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 11
AatII GACGTC 1 cut(s) 15
Acc36I ACCTGC 2 cut(s) 664, 1162
AccBSI CCGCTC 3 cut(s) 100, 263, 330
AccI GTMKAC 1 cut(s) 15
AccII CGCG 4 cut(s) 25, 51, 57, 228
AclWI GGATC 1 cut(s) 1422
AcoI YGGCCR 1 cut(s) 229
AcuI CTGAAG 4 cut(s) 1251, 1305, 1817, 1985
AcyI GRCGYC 2 cut(s) 12, 1065
AfaI GTAC 4 cut(s) 269, 338, 1243, 1687
AfiI CCNNNNNNNGG 1 cut(s) 61
AflII CTTAAG 1 cut(s) 1310
AgeI ACCGGT 1 cut(s) 1210
AgsI TTSAA 4 cut(s) 124, 424, 1460, 1943
AjnI CCWGG 2 cut(s) 108, 1621
AleI CACNNNNGTG 1 cut(s) 1134
Alw21I GWGCWC 2 cut(s) 42, 293
Alw26I GTCTC 3 cut(s) 415, 1399, 1731
Alw44I GTGCAC 1 cut(s) 289
AlwI GGATC 1 cut(s) 1422
Ama87I CYCGRG 3 cut(s) 35, 412, 1659
AoxI GGCC 1 cut(s) 229
ApaLI GTGCAC 1 cut(s) 289
ApeKI GCWGC 1 cut(s) 1442
AsiGI ACCGGT 1 cut(s) 1210
AspLEI GCGC 6 cut(s) 275, 511, 796, 838, 985, 1084
AspS9I GGNCC 4 cut(s) 31, 223, 557, 1208
AsuHPI GGTGA 9 cut(s) 172, 307, 428, 632, 668, 746, 1003, 1622, 1751
AsuII TTCGAA 3 cut(s) 473, 679, 1603
AvaI CYCGRG 3 cut(s) 35, 412, 1659
AvaII GGWCC 4 cut(s) 31, 223, 557, 1208
BaeGI GKGCMC 3 cut(s) 293, 1192, 1592
BaeI ACNNNNGTAYC 2 cut(s) 1080, 1113
BanII GRGCYC 2 cut(s) 42, 1054
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 2 cut(s) 42, 293
BbvCI CCTCAGC 1 cut(s) 1142
BbvI GCAGC 1 cut(s) 1454
BccI CCATC 7 cut(s) 638, 653, 731, 770, 1459, 1666, 1839
BceAI ACGGC 3 cut(s) 77, 260, 699
BciT130I CCWGG 2 cut(s) 110, 1623
BclI TGATCA 1 cut(s) 1477
BcoDI GTCTC 3 cut(s) 415, 1399, 1731
BfaI CTAG 4 cut(s) 194, 1278, 1718, 1767
BfmI CTRYAG 1 cut(s) 1338
BfrI CTTAAG 1 cut(s) 1310
BfuAI ACCTGC 2 cut(s) 664, 1162
BglII AGATCT 1 cut(s) 1388
BisI GCNGC 6 cut(s) 90, 98, 229, 264, 525, 1443
BlsI GCNGC 6 cut(s) 91, 99, 230, 265, 526, 1444
BmcAI AGTACT 1 cut(s) 1687
Bme1390I CCNGG 2 cut(s) 110, 1623
Bme18I GGWCC 4 cut(s) 31, 223, 557, 1208
BmeT110I CYCGRG 3 cut(s) 35, 412, 1659
BmgT120I GGNCC 4 cut(s) 31, 223, 557, 1208
BmiI GGNNCC 3 cut(s) 32, 558, 742
BmrFI CCNGG 2 cut(s) 110, 1623
BmsI GCATC 5 cut(s) 496, 540, 1216, 1259, 1546
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 395, 427
Bpu10I CCTNAGC 1 cut(s) 1142
Bpu14I TTCGAA 3 cut(s) 473, 679, 1603
BpuEI CTTGAG 1 cut(s) 1721
BsaBI GATNNNNATC 1 cut(s) 1617
BsaHI GRCGYC 2 cut(s) 12, 1065
BsaI GGTCTC 2 cut(s) 415, 1399
BsaJI CCNNGG 6 cut(s) 201, 226, 232, 276, 1132, 1178
BsaWI WCCGGW 2 cut(s) 241, 1210
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 1210
Bse1I ACTGG 4 cut(s) 119, 1317, 1512, 1882
Bse8I GATNNNNATC 1 cut(s) 1617
BseBI CCWGG 2 cut(s) 110, 1623
BseDI CCNNGG 6 cut(s) 201, 226, 232, 276, 1132, 1178
BseGI GGATG 4 cut(s) 442, 466, 649, 1714
BseJI GATNNNNATC 1 cut(s) 1617
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 8 cut(s) 154, 384, 503, 1156, 1284, 1383, 1461, 1944
BseNI ACTGG 4 cut(s) 119, 1317, 1512, 1882
BseRI GAGGAG 5 cut(s) 32, 35, 401, 767, 1843
BseSI GKGCMC 3 cut(s) 293, 1192, 1592
BseXI GCAGC 1 cut(s) 1454
BseYI CCCAGC 2 cut(s) 449, 553
BsgI GTGCAG 4 cut(s) 692, 1461, 1674, 1904
Bsh1236I CGCG 4 cut(s) 25, 51, 57, 228
Bsh1285I CGRYCG 3 cut(s) 55, 150, 213
BshFI GGCC 1 cut(s) 231
BshTI ACCGGT 1 cut(s) 1210
BsiEI CGRYCG 3 cut(s) 55, 150, 213
BsiHKAI GWGCWC 2 cut(s) 42, 293
BsiHKCI CYCGRG 3 cut(s) 35, 412, 1659
BsiSI CCGG 2 cut(s) 242, 1211
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 3 cut(s) 415, 1399, 1731
BsnI GGCC 1 cut(s) 231
Bso31I GGTCTC 2 cut(s) 415, 1399
BsoBI CYCGRG 3 cut(s) 35, 412, 1659
Bsp119I TTCGAA 3 cut(s) 473, 679, 1603
Bsp1286I GDGCHC 5 cut(s) 42, 293, 1054, 1192, 1592
Bsp143I GATC 8 cut(s) 147, 210, 352, 1112, 1388, 1414, 1477, 1960
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 8 cut(s) 153, 385, 504, 1155, 1283, 1384, 1462, 1945
BspFNI CGCG 4 cut(s) 25, 51, 57, 228
BspLI GGNNCC 3 cut(s) 32, 558, 742
BspMI ACCTGC 2 cut(s) 664, 1162
BspPI GGATC 1 cut(s) 1422
BspT104I TTCGAA 3 cut(s) 473, 679, 1603
BspTI CTTAAG 1 cut(s) 1310
BspTNI GGTCTC 2 cut(s) 415, 1399
BsrBI CCGCTC 3 cut(s) 100, 263, 330
BsrFI RCCGGY 1 cut(s) 1210
BsrI ACTGG 4 cut(s) 119, 1317, 1512, 1882
BssAI RCCGGY 1 cut(s) 1210
BssECI CCNNGG 6 cut(s) 201, 226, 232, 276, 1132, 1178
BssMI GATC 8 cut(s) 147, 210, 352, 1112, 1388, 1414, 1477, 1960
BssNI GRCGYC 2 cut(s) 12, 1065
BssT1I CCWWGG 3 cut(s) 201, 232, 1178
Bst2UI CCWGG 2 cut(s) 110, 1623
Bst4CI ACNGT 4 cut(s) 133, 295, 859, 1133
Bst6I CTCTTC 1 cut(s) 1938
BstACI GRCGYC 2 cut(s) 12, 1065
BstAFI CTTAAG 1 cut(s) 1310
BstBI TTCGAA 3 cut(s) 473, 679, 1603
BstC8I GCNNGC 5 cut(s) 404, 483, 487, 1258, 1666
BstDSI CCRYGG 3 cut(s) 226, 276, 1132
BstEII GGTNACC 1 cut(s) 178
BstF5I GGATG 4 cut(s) 442, 466, 649, 1714
BstFNI CGCG 4 cut(s) 25, 51, 57, 228
BstHHI GCGC 6 cut(s) 275, 511, 796, 838, 985, 1084
BstKTI GATC 8 cut(s) 150, 213, 355, 1115, 1391, 1417, 1480, 1963
BstMAI GTCTC 3 cut(s) 415, 1399, 1731
BstMBI GATC 8 cut(s) 147, 210, 352, 1112, 1388, 1414, 1477, 1960
BstMCI CGRYCG 3 cut(s) 55, 150, 213
BstMWI GCNNNNNNNGC 8 cut(s) 272, 692, 842, 890, 899, 1040, 1049, 1334
BstNI CCWGG 2 cut(s) 110, 1623
BstPI GGTNACC 1 cut(s) 178
BstSCI CCNGG 2 cut(s) 108, 1621
BstSFI CTRYAG 1 cut(s) 1338
BstSLI GKGCMC 3 cut(s) 293, 1192, 1592
BstUI CGCG 4 cut(s) 25, 51, 57, 228
BstV1I GCAGC 1 cut(s) 1454
BstV2I GAAGAC 1 cut(s) 134
BstX2I RGATCY 1 cut(s) 1388
BstXI CCANNNNNNTGG 2 cut(s) 1324, 1541
BstYI RGATCY 1 cut(s) 1388
BsuRI GGCC 1 cut(s) 231
BtgI CCRYGG 3 cut(s) 226, 276, 1132
BtgZI GCGATG 1 cut(s) 563
BtsCI GGATG 4 cut(s) 442, 466, 649, 1714
BtsIMutI CAGTG 3 cut(s) 1278, 1324, 1793
BveI ACCTGC 2 cut(s) 664, 1162
Cac8I GCNNGC 5 cut(s) 404, 483, 487, 1258, 1666
CfoI GCGC 6 cut(s) 275, 511, 796, 838, 985, 1084
Cfr10I RCCGGY 1 cut(s) 1210
Cfr13I GGNCC 4 cut(s) 31, 223, 557, 1208
Cfr42I CCGCGG 1 cut(s) 229
CseI GACGC 2 cut(s) 58, 1073
Csp6I GTAC 4 cut(s) 268, 337, 1242, 1686
CspAI ACCGGT 1 cut(s) 1210
CviQI GTAC 4 cut(s) 268, 337, 1242, 1686
DpnI GATC 8 cut(s) 149, 212, 354, 1114, 1390, 1416, 1479, 1962
DpnII GATC 8 cut(s) 147, 210, 352, 1112, 1388, 1414, 1477, 1960
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 1938
EarI CTCTTC 1 cut(s) 1938
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 3 cut(s) 201, 232, 1178
Eco24I GRGCYC 2 cut(s) 42, 1054
Eco31I GGTCTC 2 cut(s) 415, 1399
Eco47I GGWCC 4 cut(s) 31, 223, 557, 1208
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 4 cut(s) 1251, 1305, 1817, 1985
Eco88I CYCGRG 3 cut(s) 35, 412, 1659
Eco91I GGTNACC 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 40
EcoO109I RGGNCCY 2 cut(s) 31, 557
EcoO65I GGTNACC 1 cut(s) 178
EcoRII CCWGG 2 cut(s) 108, 1621
EcoT14I CCWWGG 3 cut(s) 201, 232, 1178
EcoT38I GRGCYC 2 cut(s) 42, 1054
ErhI CCWWGG 3 cut(s) 201, 232, 1178
FalI AAGNNNNNCTT 4 cut(s) 1293, 1325, 1901, 1933
FauI CCCGC 1 cut(s) 323
FbaI TGATCA 1 cut(s) 1477
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 6 cut(s) 90, 98, 229, 264, 525, 1443
FokI GGATG 4 cut(s) 449, 473, 656, 1721
FriOI GRGCYC 2 cut(s) 42, 1054
Fsp4HI GCNGC 6 cut(s) 90, 98, 229, 264, 525, 1443
FspBI CTAG 4 cut(s) 194, 1278, 1718, 1767
GlaI GCGC 6 cut(s) 274, 510, 795, 837, 984, 1083
GluI GCNGC 6 cut(s) 90, 98, 229, 264, 525, 1443
GsaI CCCAGC 2 cut(s) 453, 557
HaeIII GGCC 1 cut(s) 231
HapII CCGG 2 cut(s) 242, 1211
HgaI GACGC 2 cut(s) 58, 1073
HhaI GCGC 6 cut(s) 275, 511, 796, 838, 985, 1084
Hin1I GRCGYC 2 cut(s) 12, 1065
Hin6I GCGC 6 cut(s) 273, 509, 794, 836, 983, 1082
HinP1I GCGC 6 cut(s) 273, 509, 794, 836, 983, 1082
HincII GTYRAC 2 cut(s) 16, 1576
HindII GTYRAC 2 cut(s) 16, 1576
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 8 cut(s) 81, 152, 197, 206, 238, 251, 389, 1712
HpaII CCGG 2 cut(s) 242, 1211
HphI GGTGA 9 cut(s) 172, 307, 428, 632, 668, 746, 1003, 1622, 1751
Hpy166II GTNNAC 3 cut(s) 16, 291, 1576
Hpy188III TCNNGA 4 cut(s) 103, 1659, 1700, 1730
Hpy8I GTNNAC 3 cut(s) 16, 291, 1576
Hpy99I CGWCG 4 cut(s) 17, 20, 74, 286
HpyCH4III ACNGT 4 cut(s) 133, 295, 859, 1133
HpyCH4IV ACGT 6 cut(s) 12, 18, 1120, 1198, 1678, 1868
HpyF10VI GCNNNNNNNGC 8 cut(s) 272, 692, 842, 890, 899, 1040, 1049, 1334
HpySE526I ACGT 6 cut(s) 12, 18, 1120, 1198, 1678, 1868
Hsp92I GRCGYC 2 cut(s) 12, 1065
HspAI GCGC 6 cut(s) 273, 509, 794, 836, 983, 1082
Ksp22I TGATCA 1 cut(s) 1477
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 8 cut(s) 147, 210, 352, 1112, 1388, 1414, 1477, 1960
LmnI GCTCC 4 cut(s) 45, 746, 1182, 1848
Lsp1109I GCAGC 1 cut(s) 1454
LweI GCATC 5 cut(s) 496, 540, 1216, 1259, 1546
MaeI CTAG 4 cut(s) 194, 1278, 1718, 1767
MaeII ACGT 6 cut(s) 12, 18, 1120, 1198, 1678, 1868
MaeIII GTNAC 5 cut(s) 178, 244, 853, 1061, 1342
MalI GATC 8 cut(s) 149, 212, 354, 1114, 1390, 1416, 1479, 1962
MbiI CCGCTC 3 cut(s) 100, 263, 330
MboI GATC 8 cut(s) 147, 210, 352, 1112, 1388, 1414, 1477, 1960
MboII GAAGA 9 cut(s) 136, 139, 487, 1244, 1316, 1810, 1871, 1874, 1955
MfeI CAATTG 1 cut(s) 1903
MflI RGATCY 1 cut(s) 1388
MhlI GDGCHC 5 cut(s) 42, 293, 1054, 1192, 1592
MluCI AATT 8 cut(s) 746, 785, 814, 862, 910, 923, 1380, 1903
MlyI GAGTC 7 cut(s) 90, 161, 206, 215, 245, 398, 1706
MmeI TCCRAC 2 cut(s) 594, 1356
MseI TTAA 6 cut(s) 588, 861, 1215, 1311, 1839, 1871
MslI CAYNNNNRTG 4 cut(s) 1059, 1134, 1422, 1521
MspA1I CMGCKG 2 cut(s) 228, 1256
MspCI CTTAAG 1 cut(s) 1310
MspI CCGG 2 cut(s) 242, 1211
MspR9I CCNGG 2 cut(s) 110, 1623
MunI CAATTG 1 cut(s) 1903
MvaI CCWGG 2 cut(s) 110, 1623
MvnI CGCG 4 cut(s) 25, 51, 57, 228
MwoI GCNNNNNNNGC 8 cut(s) 272, 692, 842, 890, 899, 1040, 1049, 1334
NdeII GATC 8 cut(s) 147, 210, 352, 1112, 1388, 1414, 1477, 1960
NlaIV GGNNCC 3 cut(s) 32, 558, 742
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 4 cut(s) 178, 244, 1061, 1342
NspV TTCGAA 3 cut(s) 473, 679, 1603
OliI CACNNNNGTG 1 cut(s) 1134
PaeR7I CTCGAG 1 cut(s) 35
PfeI GAWTC 1 cut(s) 238
PinAI ACCGGT 1 cut(s) 1210
PkrI GCNGC 6 cut(s) 91, 99, 230, 265, 526, 1444
Ple19I CGATCG 2 cut(s) 150, 213
PleI GAGTC 7 cut(s) 89, 160, 205, 214, 245, 397, 1706
PpsI GAGTC 7 cut(s) 89, 160, 205, 214, 245, 397, 1706
PpuMI RGGWCCY 2 cut(s) 31, 557
Psp124BI GAGCTC 1 cut(s) 42
Psp5II RGGWCCY 2 cut(s) 31, 557
Psp6I CCWGG 2 cut(s) 108, 1621
PspEI GGTNACC 1 cut(s) 178
PspFI CCCAGC 2 cut(s) 449, 553
PspGI CCWGG 2 cut(s) 108, 1621
PspN4I GGNNCC 3 cut(s) 32, 558, 742
PspPI GGNCC 4 cut(s) 31, 223, 557, 1208
PspPPI RGGWCCY 2 cut(s) 31, 557
PspXI VCTCGAGB 1 cut(s) 35
PsuI RGATCY 1 cut(s) 1388
PvuI CGATCG 2 cut(s) 150, 213
PvuII CAGCTG 1 cut(s) 1256
RsaI GTAC 4 cut(s) 269, 338, 1243, 1687
RsaNI GTAC 4 cut(s) 268, 337, 1242, 1686
RseI CAYNNNNRTG 4 cut(s) 1059, 1134, 1422, 1521
SacI GAGCTC 1 cut(s) 42
SacII CCGCGG 1 cut(s) 229
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 6 cut(s) 588, 861, 1215, 1311, 1839, 1871
SatI GCNGC 6 cut(s) 90, 98, 229, 264, 525, 1443
Sau3AI GATC 8 cut(s) 147, 210, 352, 1112, 1388, 1414, 1477, 1960
Sau96I GGNCC 4 cut(s) 31, 223, 557, 1208
ScaI AGTACT 1 cut(s) 1687
SchI GAGTC 7 cut(s) 90, 161, 206, 215, 245, 398, 1706
ScrFI CCNGG 2 cut(s) 110, 1623
SduI GDGCHC 5 cut(s) 42, 293, 1054, 1192, 1592
SfaNI GCATC 5 cut(s) 496, 540, 1216, 1259, 1546
SfcI CTRYAG 1 cut(s) 1338
Sfr274I CTCGAG 1 cut(s) 35
Sfr303I CCGCGG 1 cut(s) 229
SfuI TTCGAA 3 cut(s) 473, 679, 1603
SgrBI CCGCGG 1 cut(s) 229
SgrDI CGTCGACG 1 cut(s) 14
SinI GGWCC 4 cut(s) 31, 223, 557, 1208
SlaI CTCGAG 1 cut(s) 35
SmiMI CAYNNNNRTG 4 cut(s) 1059, 1134, 1422, 1521
SmlI CTYRAG 3 cut(s) 35, 1310, 1700
SmoI CTYRAG 3 cut(s) 35, 1310, 1700
Sse9I AATT 8 cut(s) 746, 785, 814, 862, 910, 923, 1380, 1903
SspMI CTAG 4 cut(s) 194, 1278, 1718, 1767
SstI GAGCTC 1 cut(s) 42
StyD4I CCNGG 2 cut(s) 108, 1621
StyI CCWWGG 3 cut(s) 201, 232, 1178
TaaI ACNGT 4 cut(s) 133, 295, 859, 1133
TaiI ACGT 6 cut(s) 15, 21, 1123, 1201, 1681, 1871
TaqII GACCGA 1 cut(s) 166
TasI AATT 8 cut(s) 746, 785, 814, 862, 910, 923, 1380, 1903
TatI WGTACW 2 cut(s) 336, 1685
TauI GCSGC 5 cut(s) 92, 100, 231, 266, 527
TfiI GAWTC 1 cut(s) 238
Tru1I TTAA 6 cut(s) 588, 861, 1215, 1311, 1839, 1871
Tru9I TTAA 6 cut(s) 588, 861, 1215, 1311, 1839, 1871
TscAI CASTG 3 cut(s) 1278, 1324, 1800
TseFI GTSAC 4 cut(s) 178, 244, 1061, 1342
TseI GCWGC 1 cut(s) 1442
Tsp45I GTSAC 4 cut(s) 178, 244, 1061, 1342
TspDTI ATGAA 2 cut(s) 506, 1872
TspGWI ACGGA 5 cut(s) 263, 299, 552, 1259, 1620
TspRI CASTG 3 cut(s) 1278, 1324, 1800
Vha464I CTTAAG 1 cut(s) 1310
VneI GTGCAC 1 cut(s) 289
VpaK11BI GGWCC 4 cut(s) 31, 223, 557, 1208
XhoI CTCGAG 1 cut(s) 35
XmiI GTMKAC 1 cut(s) 15
XspI CTAG 4 cut(s) 194, 1278, 1718, 1767
ZraI GACGTC 1 cut(s) 13
ZrmI AGTACT 1 cut(s) 1687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.