pycom05g02580

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000492
Physical Location & Seq
Reverse (-)
3080636 .. 3080950
315 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g02580.1

Sequence Viewer

Length: 315 bp
ATGGAAAAGATGGGGTTTCATTCTTGTTGGCGCAAGATTATTATGGCGTGTGTGCATTTGGTTAACTTTGCGATACTTCTTAATGGCCAATTGGGAAACAACTTTGCTCCTCCCCGAGGATTACGCCAAGGGGATCCATTATCTCCGTATTTGTTCTTATTGGTAAGTGATGTCCTTTCTTGGAGACTTCAATCGGCAATTGATCGCAAACATTTGGAAGGCATTAAGATGAATCAGCATGCTCCCGTCATCTCCCATCTTTTCTTTGCTTATGATACACTTTTATTTTTGAAAGCTACTAGAAGGAATTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

105

Amino Acids

11.95

Weight (kDa)

9.73

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000492)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01250
fragaria_vesca FvH4_1g20711 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g28252 FvH4_3g31041 FvH4_4g00611 FvH4_5g15581 FvH4_5g21911 FvH4_5g35851 FvH4_6g24331 FvH4_6g34741
malus_domestica MD07G1291800.v1.1 MD09G1222500.v1.1
prunus_persica Prupe.1G162400_v2.0.a1 Prupe.1G210400_v2.0.a1 Prupe.6G308200_v2.0.a1
pyrus_communis pycom05g02580 pycom12g09210 pycom12g15310 pycom16g09140
rosa_chinensis RchiOBHm_Chr3g0484711 RchiOBHm_Chr4g0393411 RchiOBHm_Chr4g0397521 RchiOBHm_Chr4g0404571 RchiOBHm_Chr4g0418351 RchiOBHm_Chr5g0060311 RchiOBHm_Chr6g0301391 RchiOBHm_Chr7g0229871
rosa_laevigata RLG00000007836 RLG00000025145
rosa_multiflora Rmu_co8327795.1_g000001 Rmu_sc0000469.1_g000005 Rmu_sc0000498.1_g000050 Rmu_sc0000558.1_g000007 Rmu_sc0000693.1_g000082 Rmu_sc0000711.1_g000011 Rmu_sc0000814.1_g000039 Rmu_sc0001366.1_g000005 Rmu_sc0002205.1_g000004 Rmu_sc0002283.1_g000086 Rmu_sc0002406.1_g000011 Rmu_sc0002735.1_g000022 Rmu_sc0003545.1_g000004 Rmu_sc0004406.1_g000009 Rmu_sc0006301.1_g000009 Rmu_sc0006754.1_g000004 Rmu_sc0007324.1_g000010 Rmu_sc0008199.1_g000002 Rmu_sc0008563.1_g000009 Rmu_sc0009777.1_g000007 Rmu_sc0010560.1_g000011 Rmu_sc0011095.1_g000004 Rmu_sc0012101.1_g000004 Rmu_sc0014150.1_g000009 Rmu_sc0020270.1_g000004 Rmu_sc0021327.1_g000001 Rmu_sc0031326.1_g000003 Rmu_ssc0000116.1_g000050 Rmu_ssc0000368.1_g000056
rosa_roxburghii Rroxscaffold_2G00110080 Rroxscaffold_2G00141700 Rroxscaffold_2G00142400
rosa_rugosa Rorug01G0104400 Rorug01G0204500 Rorug02G0225600 Rorug02G0262000.1 Rorug02G0526700 Rorug03G0082000 Rorug03G0102500.1 Rorug04G0106400 Rorug04G0200200 Rorug05G0021900.1 Rorug05G0240000 Rorug05G0326500 Rorug05G0529200 Rorug07G0136100
rosa_samantha Rh1AG352100 Rh6DG494100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 128, 141
AcoI YGGCCR 1 cut(s) 85
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 2 cut(s) 191, 292
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
Alw26I GTCTC 1 cut(s) 178
AlwI GGATC 2 cut(s) 128, 141
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 1 cut(s) 85
AspLEI GCGC 1 cut(s) 33
AvaI CYCGRG 1 cut(s) 114
BalI TGGCCA 1 cut(s) 87
BamHI GGATCC 1 cut(s) 133
BccI CCATC 2 cut(s) 4, 264
BcoDI GTCTC 1 cut(s) 178
BfaI CTAG 1 cut(s) 300
BmeT110I CYCGRG 1 cut(s) 114
BmiI GGNNCC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 115, 127
Bsc4I CCNNNNNNNGG 1 cut(s) 116
BseDI CCNNGG 2 cut(s) 115, 127
BseLI CCNNNNNNNGG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 99
BshFI GGCC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 178
BsnI GGCC 1 cut(s) 87
BsoBI CYCGRG 1 cut(s) 114
Bsp143I GATC 2 cut(s) 133, 202
BspANI GGCC 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 135
BspPI GGATC 2 cut(s) 128, 141
BssECI CCNNGG 2 cut(s) 115, 127
BssMI GATC 2 cut(s) 133, 202
BssT1I CCWWGG 1 cut(s) 127
BstC8I GCNNGC 1 cut(s) 240
BstENI CCTNNNNNAGG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 2 cut(s) 136, 205
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 2 cut(s) 133, 202
BstNSI RCATGY 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BsuRI GGCC 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 240
CfoI GCGC 1 cut(s) 33
CviAII CATG 1 cut(s) 239
CviJI RGCY 2 cut(s) 87, 296
CviKI_1 RGCY 2 cut(s) 87, 296
DpnI GATC 2 cut(s) 135, 204
DpnII GATC 2 cut(s) 133, 202
EaeI YGGCCR 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 127
Eco88I CYCGRG 1 cut(s) 114
EcoNI CCTNNNNNAGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 127
FaeI CATG 1 cut(s) 242
FaiI YATR 3 cut(s) 44, 240, 273
FatI CATG 1 cut(s) 238
FspBI CTAG 1 cut(s) 300
GlaI GCGC 1 cut(s) 32
HaeIII GGCC 1 cut(s) 87
HhaI GCGC 1 cut(s) 33
Hin1II CATG 1 cut(s) 242
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HinfI GANTC 1 cut(s) 232
HpaI GTTAAC 1 cut(s) 64
Hpy166II GTNNAC 1 cut(s) 64
Hpy8I GTNNAC 1 cut(s) 64
HpyAV CCTTC 2 cut(s) 212, 297
HpyCH4V TGCA 1 cut(s) 55
Hsp92II CATG 1 cut(s) 242
HspAI GCGC 1 cut(s) 31
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 2 cut(s) 133, 202
LmnI GCTCC 2 cut(s) 112, 247
MaeI CTAG 1 cut(s) 300
MalI GATC 2 cut(s) 135, 204
MboI GATC 2 cut(s) 133, 202
MfeI CAATTG 2 cut(s) 89, 198
MflI RGATCY 1 cut(s) 133
MlsI TGGCCA 1 cut(s) 87
MluCI AATT 3 cut(s) 89, 198, 307
MluNI TGGCCA 1 cut(s) 87
MnlI CCTC 2 cut(s) 110, 120
Mox20I TGGCCA 1 cut(s) 87
MscI TGGCCA 1 cut(s) 87
MseI TTAA 3 cut(s) 63, 81, 225
MslI CAYNNNNRTG 1 cut(s) 227
Msp20I TGGCCA 1 cut(s) 87
MunI CAATTG 2 cut(s) 89, 198
NdeII GATC 2 cut(s) 133, 202
NlaIII CATG 1 cut(s) 242
NlaIV GGNNCC 1 cut(s) 135
NspI RCATGY 1 cut(s) 242
PaeI GCATGC 1 cut(s) 242
PfeI GAWTC 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 135
PsuI RGATCY 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 227
SaqAI TTAA 3 cut(s) 63, 81, 225
Sau3AI GATC 2 cut(s) 133, 202
SetI ASST 1 cut(s) 298
SgeI CNNG 9 cut(s) 36, 46, 60, 126, 128, 140, 192, 251, 257
SmiMI CAYNNNNRTG 1 cut(s) 227
SphI GCATGC 1 cut(s) 242
Sse9I AATT 3 cut(s) 89, 198, 307
SspMI CTAG 1 cut(s) 300
StyI CCWWGG 1 cut(s) 127
TasI AATT 3 cut(s) 89, 198, 307
TfiI GAWTC 1 cut(s) 232
Tru1I TTAA 3 cut(s) 63, 81, 225
Tru9I TTAA 3 cut(s) 63, 81, 225
TspDTI ATGAA 2 cut(s) 8, 245
TspGWI ACGGA 1 cut(s) 135
XagI CCTNNNNNAGG 1 cut(s) 114
XceI RCATGY 1 cut(s) 242
XspI CTAG 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.