FvH4_5g35851

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000492
Physical Location & Seq
Forward (+)
26283743 .. 26284880
1138 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35851.t1

Sequence Viewer

Length: 1050 bp
ATGAGTTTGTTTGGGTCCTACTGGCTGCTTCATGCTGATACTATTAATCATCCCAGACAAAAATTATTGCATACTACGTGGAAATTAAATGTTCCTCCTAAGGTCAAAATCTTTGCCTGGTTTTTAATTTTTAATCGTCTTAAAACCCGGGATCGATTACATGGATTTAATCCTACGATACCTTTACTTTGCCCCCTTTGTAGGACTCATTCTGAATCAGTTAATCATCTTTTTATGCATTTCCCTAGTACTTCTAATGCTTGGGCACTTTCTGGTTTTCCTGCTCCTCCTAGTAACTCGGCCATGTGTTTTTTAGATTGGATCAATACGTTTATCAATAACCCCCAGGGGCTTTCCAAATTTCTCATGTTTTGTTACACTTTATGGCGCTCTCGTAATAATGCTATTTTCCGTCATCCACCTACTTTTTCGGCTCAAATTGCCTTTGGTGCTGCTAAACAACTTGCTTTGTATCAACATGCTAATTCTGTGGTTCCTCACTCTCTAACTCATCCTTCTCTACAGGCTATTACTAATATTAAATGGCAGCCCCCTCCTCCTACTAAACTAAAAGTTAATTTTGATGGTTCTGTTATACCACCTTCTCGTGCAGCTGTTGGCTTTGTAGTTCGTCAGTCTGATGGTCTTCCGATCATTGCAACTAGTCAAAATTTAGGCCACATGGATATATTATTGGCTGAGGCGGTGGCTCTTAGAGATGGCCTTCAACTCCTTTCTCACCACACCTCCCAGGATTTACTTGTTGAAGGTGACTCTAAGCTCTTTATCGATGCTCTCACAGGAAGAACCCTTGTTCCTTGGCGCATTAAAAGGATTGTCTATGATATCATCACCTTATCAGCTCAGTTCCACTCGGTCTTTTTTAAACATGTTTGGAGAGAAGCAAACTTCTTGGCAGATAATTTTGCTAAATTAGAGCATTCTATTACTAGTCCTCAGTTGTGGGTTTCTTCTCTTCCTCTTTCCTCGACCTCTGCCTTCCACCTTGATCTTTTTAGGGGCAGTTGTAGTCGAGGTTTTTCGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

350

Amino Acids

39.41

Weight (kDa)

9.85

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 18 - 88 9.6e-16 zinc-binding in reverse transcriptase
RVT_3 PF13456 193 - 311 2.8e-19 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000492)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01250
fragaria_vesca FvH4_1g20711 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g28252 FvH4_3g31041 FvH4_4g00611 FvH4_5g15581 FvH4_5g21911 FvH4_5g35851 FvH4_6g24331 FvH4_6g34741
malus_domestica MD07G1291800.v1.1 MD09G1222500.v1.1
prunus_persica Prupe.1G162400_v2.0.a1 Prupe.1G210400_v2.0.a1 Prupe.6G308200_v2.0.a1
pyrus_communis pycom05g02580 pycom12g09210 pycom12g15310 pycom16g09140
rosa_chinensis RchiOBHm_Chr3g0484711 RchiOBHm_Chr4g0393411 RchiOBHm_Chr4g0397521 RchiOBHm_Chr4g0404571 RchiOBHm_Chr4g0418351 RchiOBHm_Chr5g0060311 RchiOBHm_Chr6g0301391 RchiOBHm_Chr7g0229871
rosa_laevigata RLG00000007836 RLG00000025145
rosa_multiflora Rmu_co8327795.1_g000001 Rmu_sc0000469.1_g000005 Rmu_sc0000498.1_g000050 Rmu_sc0000558.1_g000007 Rmu_sc0000693.1_g000082 Rmu_sc0000711.1_g000011 Rmu_sc0000814.1_g000039 Rmu_sc0001366.1_g000005 Rmu_sc0002205.1_g000004 Rmu_sc0002283.1_g000086 Rmu_sc0002406.1_g000011 Rmu_sc0002735.1_g000022 Rmu_sc0003545.1_g000004 Rmu_sc0004406.1_g000009 Rmu_sc0006301.1_g000009 Rmu_sc0006754.1_g000004 Rmu_sc0007324.1_g000010 Rmu_sc0008199.1_g000002 Rmu_sc0008563.1_g000009 Rmu_sc0009777.1_g000007 Rmu_sc0010560.1_g000011 Rmu_sc0011095.1_g000004 Rmu_sc0012101.1_g000004 Rmu_sc0014150.1_g000009 Rmu_sc0020270.1_g000004 Rmu_sc0021327.1_g000001 Rmu_sc0031326.1_g000003 Rmu_ssc0000116.1_g000050 Rmu_ssc0000368.1_g000056
rosa_roxburghii Rroxscaffold_2G00110080 Rroxscaffold_2G00141700 Rroxscaffold_2G00142400
rosa_rugosa Rorug01G0104400 Rorug01G0204500 Rorug02G0225600 Rorug02G0262000.1 Rorug02G0526700 Rorug03G0082000 Rorug03G0102500.1 Rorug04G0106400 Rorug04G0200200 Rorug05G0021900.1 Rorug05G0240000 Rorug05G0326500 Rorug05G0529200 Rorug07G0136100
rosa_samantha Rh1AG352100 Rh6DG494100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 704
AclWI GGATC 2 cut(s) 159, 329
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 2 cut(s) 359, 670
AfaI GTAC 1 cut(s) 250
AfiI CCNNNNNNNGG 1 cut(s) 201
AflIII ACRYGT 1 cut(s) 889
AgsI TTSAA 2 cut(s) 728, 767
AhlI ACTAGT 2 cut(s) 662, 950
AjnI CCWGG 3 cut(s) 116, 345, 750
AloI GAACNNNNNNTCC 2 cut(s) 799, 831
AluBI AGCT 3 cut(s) 614, 781, 863
AluI AGCT 3 cut(s) 614, 781, 863
AlwI GGATC 2 cut(s) 159, 329
Ama87I CYCGRG 1 cut(s) 147
AoxI GGCC 3 cut(s) 300, 676, 721
ApeKI GCWGC 4 cut(s) 25, 452, 547, 611
ApoI RAATTY 2 cut(s) 359, 670
AseI ATTAAT 1 cut(s) 45
AspLEI GCGC 2 cut(s) 390, 825
AspS9I GGNCC 1 cut(s) 15
AsuC2I CCSGG 2 cut(s) 148, 149
AsuHPI GGTGA 3 cut(s) 731, 782, 844
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 1 cut(s) 15
AxyI CCTNAGG 1 cut(s) 99
BaeGI GKGCMC 1 cut(s) 268
BauI CACGAG 1 cut(s) 606
BbsI GAAGAC 1 cut(s) 638
BbvCI CCTCAGC 1 cut(s) 699
BbvI GCAGC 4 cut(s) 12, 439, 559, 623
BccI CCATC 3 cut(s) 578, 635, 713
BciT130I CCWGG 3 cut(s) 118, 347, 752
BcnI CCSGG 2 cut(s) 148, 149
BcuI ACTAGT 2 cut(s) 662, 950
BfaI CTAG 4 cut(s) 246, 291, 663, 951
BfmI CTRYAG 1 cut(s) 521
BfoI RGCGCY 1 cut(s) 391
BisI GCNGC 4 cut(s) 26, 453, 548, 612
BlsI GCNGC 4 cut(s) 27, 454, 549, 613
BmcAI AGTACT 1 cut(s) 250
Bme1390I CCNGG 5 cut(s) 118, 148, 149, 347, 752
Bme18I GGWCC 1 cut(s) 15
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 15
BmiI GGNNCC 2 cut(s) 16, 495
BmrFI CCNGG 5 cut(s) 118, 148, 149, 347, 752
BmsI GCATC 1 cut(s) 781
BpiI GAAGAC 1 cut(s) 638
Bpu10I CCTNAGC 1 cut(s) 699
BpuMI CCSGG 2 cut(s) 148, 149
Bsa29I ATCGAT 2 cut(s) 154, 789
BsaAI YACGTR 1 cut(s) 78
BsaJI CCNNGG 5 cut(s) 147, 345, 346, 750, 818
BsaXI ACNNNNNCTCC 2 cut(s) 731, 761
Bsc4I CCNNNNNNNGG 1 cut(s) 201
Bse1I ACTGG 1 cut(s) 26
Bse21I CCTNAGG 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 654
BseBI CCWGG 3 cut(s) 118, 347, 752
BseCI ATCGAT 2 cut(s) 154, 789
BseDI CCNNGG 5 cut(s) 147, 345, 346, 750, 818
BseGI GGATG 3 cut(s) 49, 415, 511
BseLI CCNNNNNNNGG 1 cut(s) 201
BseMI GCAATG 1 cut(s) 654
BseMII CTCAG 3 cut(s) 690, 878, 971
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 2 cut(s) 276, 546
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 4 cut(s) 12, 439, 559, 623
BsgI GTGCAG 1 cut(s) 630
BshFI GGCC 3 cut(s) 302, 678, 723
BshVI ATCGAT 2 cut(s) 154, 789
BsiHKCI CYCGRG 1 cut(s) 147
BsiSI CCGG 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 201
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 3 cut(s) 302, 678, 723
BsoBI CYCGRG 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 4 cut(s) 151, 321, 651, 1009
BspACI CCGC 1 cut(s) 704
BspANI GGCC 3 cut(s) 302, 678, 723
BspCNI CTCAG 3 cut(s) 691, 877, 970
BspDI ATCGAT 2 cut(s) 154, 789
BspLI GGNNCC 2 cut(s) 16, 495
BspPI GGATC 2 cut(s) 159, 329
BsrDI GCAATG 1 cut(s) 654
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 5 cut(s) 147, 345, 346, 750, 818
BssMI GATC 4 cut(s) 151, 321, 651, 1009
BssSI CACGAG 1 cut(s) 606
BssT1I CCWWGG 1 cut(s) 818
Bst2BI CACGAG 1 cut(s) 606
Bst2UI CCWGG 3 cut(s) 118, 347, 752
Bst6I CTCTTC 1 cut(s) 981
BstBAI YACGTR 1 cut(s) 78
BstDEI CTNAG 6 cut(s) 99, 699, 713, 777, 864, 957
BstF5I GGATG 3 cut(s) 49, 415, 511
BstH2I RGCGCY 1 cut(s) 391
BstHHI GCGC 2 cut(s) 390, 825
BstKTI GATC 4 cut(s) 154, 324, 654, 1012
BstMBI GATC 4 cut(s) 151, 321, 651, 1009
BstMWI GCNNNNNNNGC 2 cut(s) 440, 449
BstNI CCWGG 3 cut(s) 118, 347, 752
BstNSI RCATGY 2 cut(s) 482, 893
BstSCI CCNGG 5 cut(s) 116, 146, 147, 345, 750
BstSFI CTRYAG 1 cut(s) 521
BstSLI GKGCMC 1 cut(s) 268
BstV1I GCAGC 4 cut(s) 12, 439, 559, 623
BstV2I GAAGAC 1 cut(s) 638
Bsu15I ATCGAT 2 cut(s) 154, 789
Bsu36I CCTNAGG 1 cut(s) 99
BsuRI GGCC 3 cut(s) 302, 678, 723
BsuTUI ATCGAT 2 cut(s) 154, 789
BtsCI GGATG 3 cut(s) 49, 415, 511
CfoI GCGC 2 cut(s) 390, 825
Cfr13I GGNCC 1 cut(s) 15
Cfr9I CCCGGG 1 cut(s) 147
ClaI ATCGAT 2 cut(s) 154, 789
Csp6I GTAC 1 cut(s) 249
CviAII CATG 7 cut(s) 32, 161, 304, 367, 479, 682, 890
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 6 cut(s) 99, 699, 713, 777, 864, 957
DpnI GATC 4 cut(s) 153, 323, 653, 1011
DpnII GATC 4 cut(s) 151, 321, 651, 1009
DraI TTTAAA 1 cut(s) 886
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 1 cut(s) 981
EarI CTCTTC 1 cut(s) 981
Eco130I CCWWGG 1 cut(s) 818
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 1 cut(s) 15
Eco81I CCTNAGG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 15
EcoRII CCWGG 3 cut(s) 116, 345, 750
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 1 cut(s) 818
EcoT22I ATGCAT 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 818
FaeI CATG 7 cut(s) 35, 164, 307, 370, 482, 685, 893
FatI CATG 7 cut(s) 31, 160, 303, 366, 478, 681, 889
Fnu4HI GCNGC 4 cut(s) 26, 453, 548, 612
FokI GGATG 3 cut(s) 36, 402, 498
Fsp4HI GCNGC 4 cut(s) 26, 453, 548, 612
FspBI CTAG 4 cut(s) 246, 291, 663, 951
GlaI GCGC 2 cut(s) 389, 824
GluI GCNGC 4 cut(s) 26, 453, 548, 612
HaeII RGCGCY 1 cut(s) 391
HaeIII GGCC 3 cut(s) 302, 678, 723
HapII CCGG 1 cut(s) 148
HhaI GCGC 2 cut(s) 390, 825
Hin1II CATG 7 cut(s) 35, 164, 307, 370, 482, 685, 893
Hin6I GCGC 2 cut(s) 388, 823
HinP1I GCGC 2 cut(s) 388, 823
HinfI GANTC 3 cut(s) 205, 215, 773
HpaII CCGG 1 cut(s) 148
HphI GGTGA 3 cut(s) 731, 782, 844
Hpy188I TCNGA 3 cut(s) 214, 640, 651
HpyAV CCTTC 5 cut(s) 525, 612, 734, 761, 1009
HpyCH4IV ACGT 2 cut(s) 77, 329
HpyCH4V TGCA 4 cut(s) 70, 238, 611, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 449
HpyF3I CTNAG 6 cut(s) 99, 699, 713, 777, 864, 957
HpySE526I ACGT 2 cut(s) 77, 329
Hsp92II CATG 7 cut(s) 35, 164, 307, 370, 482, 685, 893
HspAI GCGC 2 cut(s) 388, 823
Kzo9I GATC 4 cut(s) 151, 321, 651, 1009
LmnI GCTCC 1 cut(s) 289
Lsp1109I GCAGC 4 cut(s) 12, 439, 559, 623
LweI GCATC 1 cut(s) 781
MaeI CTAG 4 cut(s) 246, 291, 663, 951
MaeII ACGT 2 cut(s) 77, 329
MaeIII GTNAC 3 cut(s) 293, 374, 770
MalI GATC 4 cut(s) 153, 323, 653, 1011
MboI GATC 4 cut(s) 151, 321, 651, 1009
MboII GAAGA 4 cut(s) 638, 816, 963, 968
MhlI GDGCHC 1 cut(s) 268
MlyI GAGTC 2 cut(s) 199, 767
Mph1103I ATGCAT 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 614
MspI CCGG 1 cut(s) 148
MspR9I CCNGG 5 cut(s) 118, 148, 149, 347, 752
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 3 cut(s) 118, 347, 752
MwoI GCNNNNNNNGC 2 cut(s) 440, 449
NciI CCSGG 2 cut(s) 148, 149
NdeII GATC 4 cut(s) 151, 321, 651, 1009
NlaIII CATG 7 cut(s) 35, 164, 307, 370, 482, 685, 893
NlaIV GGNNCC 2 cut(s) 16, 495
NmeAIII GCCGAG 1 cut(s) 278
NmuCI GTSAC 1 cut(s) 770
NsiI ATGCAT 1 cut(s) 240
NspI RCATGY 2 cut(s) 482, 893
PasI CCCWGGG 1 cut(s) 346
PciI ACATGT 1 cut(s) 889
PctI GAATGC 1 cut(s) 940
PfeI GAWTC 1 cut(s) 215
PkrI GCNGC 4 cut(s) 27, 454, 549, 613
PleI GAGTC 2 cut(s) 199, 767
PpsI GAGTC 2 cut(s) 199, 767
Ppu21I YACGTR 1 cut(s) 78
PpuMI RGGWCCY 1 cut(s) 15
PscI ACATGT 1 cut(s) 889
PshBI ATTAAT 1 cut(s) 45
Psp5II RGGWCCY 1 cut(s) 15
Psp6I CCWGG 3 cut(s) 116, 345, 750
PspGI CCWGG 3 cut(s) 116, 345, 750
PspN4I GGNNCC 2 cut(s) 16, 495
PspPI GGNCC 1 cut(s) 15
PspPPI RGGWCCY 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 614
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
SatI GCNGC 4 cut(s) 26, 453, 548, 612
Sau3AI GATC 4 cut(s) 151, 321, 651, 1009
Sau96I GGNCC 1 cut(s) 15
ScaI AGTACT 1 cut(s) 250
SchI GAGTC 2 cut(s) 199, 767
ScrFI CCNGG 5 cut(s) 118, 148, 149, 347, 752
SduI GDGCHC 1 cut(s) 268
SfaNI GCATC 1 cut(s) 781
SfcI CTRYAG 1 cut(s) 521
SinI GGWCC 1 cut(s) 15
SmaI CCCGGG 1 cut(s) 149
SpeI ACTAGT 2 cut(s) 662, 950
SsiI CCGC 1 cut(s) 704
SspI AATATT 1 cut(s) 538
SspMI CTAG 4 cut(s) 246, 291, 663, 951
StyD4I CCNGG 5 cut(s) 116, 146, 147, 345, 750
StyI CCWWGG 1 cut(s) 818
TaiI ACGT 2 cut(s) 80, 332
TaqI TCGA 4 cut(s) 154, 789, 989, 1033
TaqII GACCGA 1 cut(s) 865
TatI WGTACW 1 cut(s) 248
TfiI GAWTC 1 cut(s) 215
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 4 cut(s) 25, 452, 547, 611
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 1 cut(s) 20
TspGWI ACGGA 1 cut(s) 401
TspMI CCCGGG 1 cut(s) 147
VpaK11BI GGWCC 1 cut(s) 15
VspI ATTAAT 1 cut(s) 45
XapI RAATTY 2 cut(s) 359, 670
XceI RCATGY 2 cut(s) 482, 893
XmaI CCCGGG 1 cut(s) 147
XspI CTAG 4 cut(s) 246, 291, 663, 951
ZrmI AGTACT 1 cut(s) 250
Zsp2I ATGCAT 1 cut(s) 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.