pycom16g09140

beta-mannosidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000492
Physical Location & Seq
Forward (+)
6126826 .. 6127929
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g09140.1

Sequence Viewer

Length: 1104 bp
ATGTTTCAAAATTGCTGGGATATCTTGAAAGTAGATATTTTGAAGGTGATGGAGGAGTTCTTTGAGAAAGGGATAATAAATGCGGTGACAAATGAAACATTCATTTGTCTTATTCTGAAAAAGTCAGATTCTTTGAACGTGACGGACTACAGGCCTATAAGCCTAGCTACTAGTTTATATGAGATTGTGGCGAAAGTCCTTGCATCAAGACTAGAGGACGTTATGGGTAGCACAATCTCTCTAAATCAAGGAGCTTTTGTTAAAGGTAGACAAATTTTGGATGTGGTCCTTGCAAATGAGGTTGTAGAAGAGGTGAGGCAAAAGAAAAAGGAGGGATTGATGTTCAAGATTGATTTTGAAAAGGCTTATGATCATGTGGAGTGGAGATTTTTGGATGAAGTCTTACAAAGAAAAGGCTTTGGCAATAGATGGAGGAGAAGGGTGCAAGGTTGTCTAAGTTCCGCAAATTTCTCAGTTTTGATCAATGGGAGGCCTAGAGGAAAATTTAAAGCTTCAAGAGGTTTGAGACAAGGTAATCCACTGTCACCTTTTCTTTTCACCTTATTAGTTGATGTCTTGAGTAGATTGCTGGAGAAGGCACAAGAGAATAATATGATCAAAGGTTTGTGCATTGGGCAAGAAAAGGTGGAGATTTTGCATCTTCAATTTGCTAATGACACCATTTTTTTCTTGGCGGGGGCTGAGGGAGTTTGGAAAAACTTATTAGAGTTGCCCAATTTATTTTGTACTGTTTCAAGTTTGAAAATTAACAAAGCAAAGTGCTCTTTGGCTGGAATAAACTGGGATTGTGAGAAAATTAAAAGGATGGTAGATTCTTGGGGTTGTGAGGTGGGTAGTTGGCCAATAACATATTTGTGTCTCCCTTTTGGGGGAAGGCCAAGAGCACTTACGTTTTGGGATCCAGTTGTAGAAAAGATGGAAAAAAGACTTCAAAGTTGTAAGAATGATTTCTTATCTAGAGGTGGTAGATTAACTTTGATTCAATCTGTTTTCGGAAGTTTGCCAATTTATTACATGTCTTTGTTCAAAATTCCTTGCGGGGTGACTGGACGGCTGGAAAAACTACTGAAATGGTTTCTTTAG

Protein Analysis

368

Amino Acids

41.93

Weight (kDa)

9.22

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 40 - 265 1.1e-30 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000492)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01250
fragaria_vesca FvH4_1g20711 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g28252 FvH4_3g31041 FvH4_4g00611 FvH4_5g15581 FvH4_5g21911 FvH4_5g35851 FvH4_6g24331 FvH4_6g34741
malus_domestica MD07G1291800.v1.1 MD09G1222500.v1.1
prunus_persica Prupe.1G162400_v2.0.a1 Prupe.1G210400_v2.0.a1 Prupe.6G308200_v2.0.a1
pyrus_communis pycom05g02580 pycom12g09210 pycom12g15310 pycom16g09140
rosa_chinensis RchiOBHm_Chr3g0484711 RchiOBHm_Chr4g0393411 RchiOBHm_Chr4g0397521 RchiOBHm_Chr4g0404571 RchiOBHm_Chr4g0418351 RchiOBHm_Chr5g0060311 RchiOBHm_Chr6g0301391 RchiOBHm_Chr7g0229871
rosa_laevigata RLG00000007836 RLG00000025145
rosa_multiflora Rmu_co8327795.1_g000001 Rmu_sc0000469.1_g000005 Rmu_sc0000498.1_g000050 Rmu_sc0000558.1_g000007 Rmu_sc0000693.1_g000082 Rmu_sc0000711.1_g000011 Rmu_sc0000814.1_g000039 Rmu_sc0001366.1_g000005 Rmu_sc0002205.1_g000004 Rmu_sc0002283.1_g000086 Rmu_sc0002406.1_g000011 Rmu_sc0002735.1_g000022 Rmu_sc0003545.1_g000004 Rmu_sc0004406.1_g000009 Rmu_sc0006301.1_g000009 Rmu_sc0006754.1_g000004 Rmu_sc0007324.1_g000010 Rmu_sc0008199.1_g000002 Rmu_sc0008563.1_g000009 Rmu_sc0009777.1_g000007 Rmu_sc0010560.1_g000011 Rmu_sc0011095.1_g000004 Rmu_sc0012101.1_g000004 Rmu_sc0014150.1_g000009 Rmu_sc0020270.1_g000004 Rmu_sc0021327.1_g000001 Rmu_sc0031326.1_g000003 Rmu_ssc0000116.1_g000050 Rmu_ssc0000368.1_g000056
rosa_roxburghii Rroxscaffold_2G00110080 Rroxscaffold_2G00141700 Rroxscaffold_2G00142400
rosa_rugosa Rorug01G0104400 Rorug01G0204500 Rorug02G0225600 Rorug02G0262000.1 Rorug02G0526700 Rorug03G0082000 Rorug03G0102500.1 Rorug04G0106400 Rorug04G0200200 Rorug05G0021900.1 Rorug05G0240000 Rorug05G0326500 Rorug05G0529200 Rorug07G0136100
rosa_samantha Rh1AG352100 Rh6DG494100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 268
AciI CCGC 4 cut(s) 83, 462, 695, 1059
AclWI GGATC 2 cut(s) 914, 927
AcoI YGGCCR 1 cut(s) 860
AcsI RAATTY 4 cut(s) 273, 466, 503, 1050
AfaI GTAC 1 cut(s) 748
AfiI CCNNNNNNNGG 2 cut(s) 889, 890
AflIII ACRYGT 1 cut(s) 1035
AhlI ACTAGT 1 cut(s) 170
AluBI AGCT 3 cut(s) 167, 254, 512
AluI AGCT 3 cut(s) 167, 254, 512
Alw21I GWGCWC 2 cut(s) 785, 907
Alw26I GTCTC 2 cut(s) 520, 884
AlwI GGATC 2 cut(s) 914, 927
AoxI GGCC 4 cut(s) 152, 491, 860, 896
ApoI RAATTY 4 cut(s) 273, 466, 503, 1050
Asp700I GAANNNNTTC 1 cut(s) 968
AspS9I GGNCC 1 cut(s) 286
AsuHPI GGTGA 6 cut(s) 58, 97, 325, 537, 550, 1075
AvaII GGWCC 1 cut(s) 286
BalI TGGCCA 1 cut(s) 862
BamHI GGATCC 1 cut(s) 919
Bbv12I GWGCWC 2 cut(s) 785, 907
BbvCI CCTCAGC 1 cut(s) 702
BccI CCATC 4 cut(s) 43, 423, 820, 931
BceAI ACGGC 1 cut(s) 1088
BclI TGATCA 3 cut(s) 370, 480, 615
BcoDI GTCTC 2 cut(s) 520, 884
BcuI ACTAGT 1 cut(s) 170
BfaI CTAG 5 cut(s) 164, 171, 212, 495, 978
BfmI CTRYAG 1 cut(s) 148
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 921
BmrI ACTGGG 1 cut(s) 811
BmsI GCATC 2 cut(s) 212, 667
BmuI ACTGGG 1 cut(s) 811
BpmI CTGGAG 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 598
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 889, 890
Bse1I ACTGG 3 cut(s) 806, 923, 1072
BseGI GGATG 3 cut(s) 286, 400, 831
BseLI CCNNNNNNNGG 2 cut(s) 889, 890
BseMII CTCAG 2 cut(s) 486, 693
BseNI ACTGG 3 cut(s) 806, 923, 1072
BseRI GAGGAG 2 cut(s) 68, 448
BseYI CCCAGC 1 cut(s) 15
BshFI GGCC 4 cut(s) 154, 493, 862, 898
BsiHKAI GWGCWC 2 cut(s) 785, 907
BslI CCNNNNNNNGG 2 cut(s) 889, 890
BsmAI GTCTC 2 cut(s) 520, 884
BsnI GGCC 4 cut(s) 154, 493, 862, 898
Bsp1286I GDGCHC 2 cut(s) 785, 907
Bsp143I GATC 4 cut(s) 370, 480, 615, 919
BspACI CCGC 4 cut(s) 83, 462, 695, 1059
BspANI GGCC 4 cut(s) 154, 493, 862, 898
BspCNI CTCAG 2 cut(s) 485, 694
BspLI GGNNCC 1 cut(s) 921
BspPI GGATC 2 cut(s) 914, 927
BsrI ACTGG 3 cut(s) 806, 923, 1072
BssMI GATC 4 cut(s) 370, 480, 615, 919
Bst4CI ACNGT 2 cut(s) 543, 751
Bst6I CTCTTC 1 cut(s) 303
BstDEI CTNAG 3 cut(s) 455, 472, 702
BstF5I GGATG 3 cut(s) 286, 400, 831
BstKTI GATC 4 cut(s) 373, 483, 618, 922
BstMAI GTCTC 2 cut(s) 520, 884
BstMBI GATC 4 cut(s) 370, 480, 615, 919
BstNSI RCATGY 1 cut(s) 1039
BstSFI CTRYAG 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 919
BstYI RGATCY 1 cut(s) 919
BsuRI GGCC 4 cut(s) 154, 493, 862, 898
BtsCI GGATG 3 cut(s) 286, 400, 831
BtsIMutI CAGTG 1 cut(s) 539
Cfr13I GGNCC 1 cut(s) 286
Csp6I GTAC 1 cut(s) 747
CviAII CATG 2 cut(s) 374, 1036
CviQI GTAC 1 cut(s) 747
DdeI CTNAG 3 cut(s) 455, 472, 702
DpnI GATC 4 cut(s) 372, 482, 617, 921
DpnII GATC 4 cut(s) 370, 480, 615, 919
DraI TTTAAA 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 860
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
Eco147I AGGCCT 2 cut(s) 154, 493
Eco32I GATATC 1 cut(s) 22
Eco47I GGWCC 1 cut(s) 286
EcoRV GATATC 1 cut(s) 22
FaeI CATG 2 cut(s) 377, 1039
FaiI YATR 9 cut(s) 158, 178, 180, 224, 369, 375, 614, 871, 1037
FatI CATG 2 cut(s) 373, 1035
FauI CCCGC 2 cut(s) 688, 1052
FbaI TGATCA 3 cut(s) 370, 480, 615
FblI GTMKAC 1 cut(s) 268
FokI GGATG 3 cut(s) 293, 407, 838
FspBI CTAG 5 cut(s) 164, 171, 212, 495, 978
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 611
HaeIII GGCC 4 cut(s) 154, 493, 862, 898
Hin1II CATG 2 cut(s) 377, 1039
HindIII AAGCTT 1 cut(s) 510
HinfI GANTC 3 cut(s) 128, 833, 1000
HphI GGTGA 6 cut(s) 58, 97, 325, 537, 550, 1075
Hpy166II GTNNAC 1 cut(s) 269
Hpy188I TCNGA 3 cut(s) 117, 127, 1016
Hpy188III TCNNGA 6 cut(s) 25, 207, 346, 516, 577, 978
Hpy8I GTNNAC 1 cut(s) 269
HpyAV CCTTC 4 cut(s) 37, 432, 589, 888
HpyCH4III ACNGT 2 cut(s) 543, 751
HpyCH4IV ACGT 3 cut(s) 138, 219, 911
HpyCH4V TGCA 5 cut(s) 203, 293, 445, 630, 658
HpyF3I CTNAG 3 cut(s) 455, 472, 702
HpySE526I ACGT 3 cut(s) 138, 219, 911
Hsp92II CATG 2 cut(s) 377, 1039
Ksp22I TGATCA 3 cut(s) 370, 480, 615
Kzo9I GATC 4 cut(s) 370, 480, 615, 919
LmnI GCTCC 1 cut(s) 251
LpnPI CCDG 7 cut(s) 136, 575, 777, 787, 936, 1053, 1061
LweI GCATC 2 cut(s) 212, 667
MaeI CTAG 5 cut(s) 164, 171, 212, 495, 978
MaeII ACGT 3 cut(s) 138, 219, 911
MaeIII GTNAC 4 cut(s) 85, 139, 543, 1063
MalI GATC 4 cut(s) 372, 482, 617, 921
MboI GATC 4 cut(s) 370, 480, 615, 919
MboII GAAGA 2 cut(s) 320, 653
MflI RGATCY 1 cut(s) 919
MhlI GDGCHC 2 cut(s) 785, 907
MlsI TGGCCA 1 cut(s) 862
MluNI TGGCCA 1 cut(s) 862
Mox20I TGGCCA 1 cut(s) 862
MroXI GAANNNNTTC 1 cut(s) 968
MscI TGGCCA 1 cut(s) 862
MseI TTAA 5 cut(s) 261, 507, 768, 819, 992
MslI CAYNNNNRTG 1 cut(s) 874
Msp20I TGGCCA 1 cut(s) 862
NdeII GATC 4 cut(s) 370, 480, 615, 919
NlaIII CATG 2 cut(s) 377, 1039
NlaIV GGNNCC 1 cut(s) 921
NmuCI GTSAC 4 cut(s) 85, 139, 543, 1063
NspI RCATGY 1 cut(s) 1039
PceI AGGCCT 2 cut(s) 154, 493
PciI ACATGT 1 cut(s) 1035
PdmI GAANNNNTTC 1 cut(s) 968
PfeI GAWTC 3 cut(s) 128, 833, 1000
PscI ACATGT 1 cut(s) 1035
PspFI CCCAGC 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 921
PspPI GGNCC 1 cut(s) 286
PsuI RGATCY 1 cut(s) 919
RsaI GTAC 1 cut(s) 748
RsaNI GTAC 1 cut(s) 747
RseI CAYNNNNRTG 1 cut(s) 874
SaqAI TTAA 5 cut(s) 261, 507, 768, 819, 992
Sau3AI GATC 4 cut(s) 370, 480, 615, 919
Sau96I GGNCC 1 cut(s) 286
SduI GDGCHC 2 cut(s) 785, 907
SfaNI GCATC 2 cut(s) 212, 667
SfcI CTRYAG 1 cut(s) 148
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 874
SmlI CTYRAG 1 cut(s) 577
SmoI CTYRAG 1 cut(s) 577
SpeI ACTAGT 1 cut(s) 170
SseBI AGGCCT 2 cut(s) 154, 493
SsiI CCGC 4 cut(s) 83, 462, 695, 1059
SspMI CTAG 5 cut(s) 164, 171, 212, 495, 978
StuI AGGCCT 2 cut(s) 154, 493
TaaI ACNGT 2 cut(s) 543, 751
TaiI ACGT 3 cut(s) 141, 222, 914
TatI WGTACW 1 cut(s) 746
TfiI GAWTC 3 cut(s) 128, 833, 1000
Tru1I TTAA 5 cut(s) 261, 507, 768, 819, 992
Tru9I TTAA 5 cut(s) 261, 507, 768, 819, 992
TscAI CASTG 1 cut(s) 546
TseFI GTSAC 4 cut(s) 85, 139, 543, 1063
Tsp45I GTSAC 4 cut(s) 85, 139, 543, 1063
TspDTI ATGAA 3 cut(s) 91, 108, 411
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 286
XapI RAATTY 4 cut(s) 273, 466, 503, 1050
XbaI TCTAGA 1 cut(s) 977
XceI RCATGY 1 cut(s) 1039
XcmI CCANNNNNNNNNTGG 1 cut(s) 688
XmiI GTMKAC 1 cut(s) 268
XmnI GAANNNNTTC 1 cut(s) 968
XspI CTAG 5 cut(s) 164, 171, 212, 495, 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.