Rmu_sc0003545.1_g000004

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003545.1
OG0000492
Physical Location & Seq
Forward (+)
10912 .. 13471
2560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003545.1_g000004.1.cds

Sequence Viewer

Length: 1056 bp
atggaggcgaggatttcagggcacataccgactaggcggcggaactcgggggtggagaagcccattgaggtgaggaaatggaaggtggatttgaggtcgaggagggaggcggagatggttggagggagctatgcctatgggtgcccaaatcagatcttggcctcgccggcctctgactcgtgggttgctgccgaatgtcatggaggctgcgttgaactcgtcgaaggagatgaggattctcgttcggggagcgcagggaggattctgaagctttcctgttgggagaaacccttgtggtggcggcgaagggttgaggatgctgtggagggagggggctctcctaaagctgctcggaatatcacaaggattctttctagatttgttgcttgctttgggcagcacattaattcagataagtcctctatctatttctcctctaacttaaatgcttcaactaaacaacagactgctaatatattgggtattcaacatagaactactataggcaaatatctaggtgttcataacattatattctggaaagatcccctgaatgctaaagagttgcttgttagaatttcaaaaaagcttagtggttggaagagttccactctatctagggctgggcgtctcactcttttaaagtcaagcgcttctgcggtgcctaaccatgttgtttcttgctttaaatgccctacttctgtttcaaagtccatagataaggaaatgaggaatttcttttggggcagctctagttcccctccggttgcttgggagcaagtttgcttgccgaagtctaaaggagggcttggtattagaccggtttcttgttttaataaagctgccttggccaagctaggttggaaagttattacagaccaaaataactggtgggtggagattgttaggaaaaaatacctttgtaggttctcattttttgaggttcctaagcaatctcatcagtcgtttgcttggaagggtattttggactctagagatattattctgaaaggaatgagatggattattgggaatggtcataacataaaattttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

351

Amino Acids

39.34

Weight (kDa)

9.98

Isoelectric Point (pI)

63.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000492)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01250
fragaria_vesca FvH4_1g20711 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g28252 FvH4_3g31041 FvH4_4g00611 FvH4_5g15581 FvH4_5g21911 FvH4_5g35851 FvH4_6g24331 FvH4_6g34741
malus_domestica MD07G1291800.v1.1 MD09G1222500.v1.1
prunus_persica Prupe.1G162400_v2.0.a1 Prupe.1G210400_v2.0.a1 Prupe.6G308200_v2.0.a1
pyrus_communis pycom05g02580 pycom12g09210 pycom12g15310 pycom16g09140
rosa_chinensis RchiOBHm_Chr3g0484711 RchiOBHm_Chr4g0393411 RchiOBHm_Chr4g0397521 RchiOBHm_Chr4g0404571 RchiOBHm_Chr4g0418351 RchiOBHm_Chr5g0060311 RchiOBHm_Chr6g0301391 RchiOBHm_Chr7g0229871
rosa_laevigata RLG00000007836 RLG00000025145
rosa_multiflora Rmu_co8327795.1_g000001 Rmu_sc0000469.1_g000005 Rmu_sc0000498.1_g000050 Rmu_sc0000558.1_g000007 Rmu_sc0000693.1_g000082 Rmu_sc0000711.1_g000011 Rmu_sc0000814.1_g000039 Rmu_sc0001366.1_g000005 Rmu_sc0002205.1_g000004 Rmu_sc0002283.1_g000086 Rmu_sc0002406.1_g000011 Rmu_sc0002735.1_g000022 Rmu_sc0003545.1_g000004 Rmu_sc0004406.1_g000009 Rmu_sc0006301.1_g000009 Rmu_sc0006754.1_g000004 Rmu_sc0007324.1_g000010 Rmu_sc0008199.1_g000002 Rmu_sc0008563.1_g000009 Rmu_sc0009777.1_g000007 Rmu_sc0010560.1_g000011 Rmu_sc0011095.1_g000004 Rmu_sc0012101.1_g000004 Rmu_sc0014150.1_g000009 Rmu_sc0020270.1_g000004 Rmu_sc0021327.1_g000001 Rmu_sc0031326.1_g000003 Rmu_ssc0000116.1_g000050 Rmu_ssc0000368.1_g000056
rosa_roxburghii Rroxscaffold_2G00110080 Rroxscaffold_2G00141700 Rroxscaffold_2G00142400
rosa_rugosa Rorug01G0104400 Rorug01G0204500 Rorug02G0225600 Rorug02G0262000.1 Rorug02G0526700 Rorug03G0082000 Rorug03G0102500.1 Rorug04G0106400 Rorug04G0200200 Rorug05G0021900.1 Rorug05G0240000 Rorug05G0326500 Rorug05G0529200 Rorug07G0136100
rosa_samantha Rh1AG352100 Rh6DG494100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 141, 661
AciI CCGC 5 cut(s) 37, 40, 110, 301, 659
AclWI GGATC 1 cut(s) 539
AcoI YGGCCR 1 cut(s) 849
AcsI RAATTY 3 cut(s) 576, 733, 1049
AcuI CTGAAG 1 cut(s) 287
AcyI GRCGYC 1 cut(s) 628
AfeI AGCGCT 1 cut(s) 652
AfiI CCNNNNNNNGG 1 cut(s) 297
AgeI ACCGGT 1 cut(s) 820
AgsI TTSAA 5 cut(s) 215, 453, 488, 582, 708
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
AluBI AGCT 7 cut(s) 129, 271, 347, 589, 750, 842, 856
AluI AGCT 7 cut(s) 129, 271, 347, 589, 750, 842, 856
Alw26I GTCTC 1 cut(s) 635
AlwI GGATC 1 cut(s) 539
Ama87I CYCGRG 1 cut(s) 46
Aor51HI AGCGCT 1 cut(s) 652
AoxI GGCC 3 cut(s) 159, 168, 849
ApeKI GCWGC 6 cut(s) 188, 207, 347, 397, 747, 842
ApoI RAATTY 3 cut(s) 576, 733, 1049
AseI ATTAAT 1 cut(s) 405
AsiGI ACCGGT 1 cut(s) 820
AspLEI GCGC 2 cut(s) 254, 653
AsuHPI GGTGA 1 cut(s) 82
AvaI CYCGRG 1 cut(s) 46
BaeGI GKGCMC 2 cut(s) 24, 146
BalI TGGCCA 1 cut(s) 851
BanI GGYRCC 2 cut(s) 141, 661
BanII GRGCYC 1 cut(s) 338
BauI CACGAG 1 cut(s) 178
BbvI GCAGC 6 cut(s) 175, 194, 334, 409, 759, 829
BccI CCATC 2 cut(s) 109, 1014
BcoDI GTCTC 1 cut(s) 635
BfaI CTAG 7 cut(s) 33, 375, 515, 618, 753, 857, 993
BfmI CTRYAG 1 cut(s) 501
BfoI RGCGCY 1 cut(s) 654
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 1 cut(s) 153
BisI GCNGC 8 cut(s) 38, 189, 208, 302, 348, 398, 748, 843
BlsI GCNGC 8 cut(s) 39, 190, 209, 303, 349, 399, 749, 844
BmeT110I CYCGRG 1 cut(s) 46
BmiI GGNNCC 3 cut(s) 143, 663, 945
BmsI GCATC 1 cut(s) 307
BplI GAGNNNNNCTC 2 cut(s) 595, 627
Bpu10I CCTNAGC 1 cut(s) 948
BsaHI GRCGYC 1 cut(s) 628
BsaJI CCNNGG 1 cut(s) 846
BsaWI WCCGGW 2 cut(s) 763, 820
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse118I RCCGGY 2 cut(s) 166, 820
Bse1I ACTGG 1 cut(s) 893
BseDI CCNNGG 1 cut(s) 846
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 893
BseRI GAGGAG 2 cut(s) 115, 424
BseSI GKGCMC 2 cut(s) 24, 146
BseXI GCAGC 6 cut(s) 175, 194, 334, 409, 759, 829
BseYI CCCAGC 1 cut(s) 623
BshFI GGCC 3 cut(s) 161, 170, 851
BshNI GGYRCC 2 cut(s) 141, 661
BshTI ACCGGT 1 cut(s) 820
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 3 cut(s) 167, 764, 821
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 635
BsmBI CGTCTC 1 cut(s) 635
BsmI GAATGC 1 cut(s) 559
BsnI GGCC 3 cut(s) 161, 170, 851
BsoBI CYCGRG 1 cut(s) 46
Bsp1286I GDGCHC 3 cut(s) 24, 146, 338
Bsp143I GATC 2 cut(s) 153, 544
BspACI CCGC 5 cut(s) 37, 40, 110, 301, 659
BspANI GGCC 3 cut(s) 161, 170, 851
BspLI GGNNCC 3 cut(s) 143, 663, 945
BspPI GGATC 1 cut(s) 539
BspT107I GGYRCC 2 cut(s) 141, 661
BsrFI RCCGGY 2 cut(s) 166, 820
BsrI ACTGG 1 cut(s) 893
BssAI RCCGGY 2 cut(s) 166, 820
BssECI CCNNGG 1 cut(s) 846
BssMI GATC 2 cut(s) 153, 544
BssNI GRCGYC 1 cut(s) 628
BssSI CACGAG 1 cut(s) 178
BssT1I CCWWGG 1 cut(s) 846
Bst2BI CACGAG 1 cut(s) 178
Bst6I CTCTTC 1 cut(s) 596
BstACI GRCGYC 1 cut(s) 628
BstC8I GCNNGC 3 cut(s) 168, 388, 788
BstDEI CTNAG 2 cut(s) 590, 948
BstF5I GGATG 1 cut(s) 322
BstH2I RGCGCY 1 cut(s) 654
BstHHI GCGC 2 cut(s) 254, 653
BstKTI GATC 2 cut(s) 156, 547
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 2 cut(s) 153, 544
BstMWI GCNNNNNNNGC 3 cut(s) 167, 690, 848
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 2 cut(s) 24, 146
BstV1I GCAGC 6 cut(s) 175, 194, 334, 409, 759, 829
BstX2I RGATCY 2 cut(s) 153, 544
BstYI RGATCY 2 cut(s) 153, 544
BsuRI GGCC 3 cut(s) 161, 170, 851
BtsCI GGATG 1 cut(s) 322
Cac8I GCNNGC 3 cut(s) 168, 388, 788
CfoI GCGC 2 cut(s) 254, 653
Cfr10I RCCGGY 2 cut(s) 166, 820
CseI GACGC 1 cut(s) 617
CspAI ACCGGT 1 cut(s) 820
CviAII CATG 2 cut(s) 200, 671
DdeI CTNAG 2 cut(s) 590, 948
DpnI GATC 2 cut(s) 155, 546
DpnII GATC 2 cut(s) 153, 544
DraI TTTAAA 2 cut(s) 642, 688
EaeI YGGCCR 1 cut(s) 849
Eam1104I CTCTTC 1 cut(s) 596
EarI CTCTTC 1 cut(s) 596
EciI GGCGGA 2 cut(s) 55, 125
Eco130I CCWWGG 1 cut(s) 846
Eco24I GRGCYC 1 cut(s) 338
Eco47III AGCGCT 1 cut(s) 652
Eco57I CTGAAG 1 cut(s) 287
Eco88I CYCGRG 1 cut(s) 46
EcoT14I CCWWGG 1 cut(s) 846
EcoT38I GRGCYC 1 cut(s) 338
ErhI CCWWGG 1 cut(s) 846
Esp3I CGTCTC 1 cut(s) 635
FaeI CATG 2 cut(s) 203, 674
FalI AAGNNNNNCTT 4 cut(s) 552, 584, 792, 824
FatI CATG 2 cut(s) 199, 670
Fnu4HI GCNGC 8 cut(s) 38, 189, 208, 302, 348, 398, 748, 843
FokI GGATG 1 cut(s) 329
FriOI GRGCYC 1 cut(s) 338
Fsp4HI GCNGC 8 cut(s) 38, 189, 208, 302, 348, 398, 748, 843
FspBI CTAG 7 cut(s) 33, 375, 515, 618, 753, 857, 993
GlaI GCGC 2 cut(s) 253, 652
GluI GCNGC 8 cut(s) 38, 189, 208, 302, 348, 398, 748, 843
GsaI CCCAGC 1 cut(s) 627
HaeII RGCGCY 1 cut(s) 654
HaeIII GGCC 3 cut(s) 161, 170, 851
HapII CCGG 3 cut(s) 167, 764, 821
HgaI GACGC 1 cut(s) 617
HhaI GCGC 2 cut(s) 254, 653
Hin1I GRCGYC 1 cut(s) 628
Hin1II CATG 2 cut(s) 203, 674
Hin6I GCGC 2 cut(s) 252, 651
HinP1I GCGC 2 cut(s) 252, 651
HindIII AAGCTT 2 cut(s) 269, 587
HinfI GANTC 5 cut(s) 176, 236, 262, 367, 989
HpaII CCGG 3 cut(s) 167, 764, 821
HphI GGTGA 1 cut(s) 82
Hpy188I TCNGA 6 cut(s) 153, 175, 267, 354, 412, 1008
Hpy188III TCNNGA 3 cut(s) 375, 538, 993
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 4 cut(s) 76, 218, 300, 970
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 690, 848
HpyF3I CTNAG 2 cut(s) 590, 948
Hsp92I GRCGYC 1 cut(s) 628
Hsp92II CATG 2 cut(s) 203, 674
HspAI GCGC 2 cut(s) 252, 651
KroI GCCGGC 1 cut(s) 166
KroNI GCCGGC 1 cut(s) 168
Kzo9I GATC 2 cut(s) 153, 544
LmnI GCTCC 3 cut(s) 126, 249, 775
Lsp1109I GCAGC 6 cut(s) 175, 194, 334, 409, 759, 829
LweI GCATC 1 cut(s) 307
MaeI CTAG 7 cut(s) 33, 375, 515, 618, 753, 857, 993
MalI GATC 2 cut(s) 155, 546
MboI GATC 2 cut(s) 153, 544
MboII GAAGA 1 cut(s) 613
MflI RGATCY 2 cut(s) 153, 544
MhlI GDGCHC 3 cut(s) 24, 146, 338
MlsI TGGCCA 1 cut(s) 851
MluCI AATT 4 cut(s) 406, 576, 733, 1049
MluNI TGGCCA 1 cut(s) 851
MlyI GAGTC 2 cut(s) 170, 983
MmeI TCCRAC 3 cut(s) 100, 578, 842
Mox20I TGGCCA 1 cut(s) 851
MroNI GCCGGC 1 cut(s) 166
MscI TGGCCA 1 cut(s) 851
MseI TTAA 5 cut(s) 405, 443, 641, 687, 834
MslI CAYNNNNRTG 1 cut(s) 68
Msp20I TGGCCA 1 cut(s) 851
MspI CCGG 3 cut(s) 167, 764, 821
Mva1269I GAATGC 1 cut(s) 559
MwoI GCNNNNNNNGC 3 cut(s) 167, 690, 848
NaeI GCCGGC 1 cut(s) 168
NdeII GATC 2 cut(s) 153, 544
NgoMIV GCCGGC 1 cut(s) 166
NlaIII CATG 2 cut(s) 203, 674
NlaIV GGNNCC 3 cut(s) 143, 663, 945
PctI GAATGC 1 cut(s) 559
PdiI GCCGGC 1 cut(s) 168
PfeI GAWTC 3 cut(s) 236, 262, 367
PinAI ACCGGT 1 cut(s) 820
PkrI GCNGC 8 cut(s) 39, 190, 209, 303, 349, 399, 749, 844
PleI GAGTC 2 cut(s) 170, 983
PpsI GAGTC 2 cut(s) 170, 983
PshBI ATTAAT 1 cut(s) 405
PspFI CCCAGC 1 cut(s) 623
PspN4I GGNNCC 3 cut(s) 143, 663, 945
PsuI RGATCY 2 cut(s) 153, 544
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 5 cut(s) 405, 443, 641, 687, 834
SatI GCNGC 8 cut(s) 38, 189, 208, 302, 348, 398, 748, 843
Sau3AI GATC 2 cut(s) 153, 544
SchI GAGTC 2 cut(s) 170, 983
SduI GDGCHC 3 cut(s) 24, 146, 338
SfaNI GCATC 1 cut(s) 307
SfcI CTRYAG 1 cut(s) 501
SfiI GGCCNNNNNGGCC 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 68
Sse9I AATT 4 cut(s) 406, 576, 733, 1049
SsiI CCGC 5 cut(s) 37, 40, 110, 301, 659
SspMI CTAG 7 cut(s) 33, 375, 515, 618, 753, 857, 993
StyI CCWWGG 1 cut(s) 846
TaqI TCGA 2 cut(s) 98, 222
TasI AATT 4 cut(s) 406, 576, 733, 1049
TauI GCSGC 2 cut(s) 40, 304
TfiI GAWTC 3 cut(s) 236, 262, 367
Tru1I TTAA 5 cut(s) 405, 443, 641, 687, 834
Tru9I TTAA 5 cut(s) 405, 443, 641, 687, 834
TseI GCWGC 6 cut(s) 188, 207, 347, 397, 747, 842
TspDTI ATGAA 1 cut(s) 512
VspI ATTAAT 1 cut(s) 405
XapI RAATTY 3 cut(s) 576, 733, 1049
XbaI TCTAGA 2 cut(s) 374, 992
XspI CTAG 7 cut(s) 33, 375, 515, 618, 753, 857, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.