RchiOBHm_Chr4g0393411

ribonuclease H protein At1g65750

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000492
Physical Location & Seq
Reverse (-)
8899746 .. 8907087
7342 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36601

Sequence Viewer

Length: 1791 bp
ATGTGGTTGGAACATCCTTCCTTCAAAGAAAACTTTAGAAAGTGGTGGGATGAAGGAAACTGTGATGGCTGGAAAGGCTTTAGATTCATGAGAAAGCTCAAGAATGTGAAAGGAAAATTAAAGATATGGAGTCGAGAGACTTTTGGTGATATTGGGAAAGAAAAGAAAGTGGTGGAAGCAAGAATAAACGAGCTGGATGAGGAAGAAAGGAGTGCAGGTATATGTGTGGTCAAAAAAAGGGAGAGGGAAGTTTTACGAGGGCGACTGGAGGAGTTAGCCCTGAGAGAAGAGATTTTCTGGAGGCAAAGAGCTAAGCTAAAATGGGCCAAAGAAGGAGACAACAACACAAGATTCTTCCACAAGCTAGTCAATGGCAGAAGAAAAAGAAATGTTATTGAAAAGCTGGAATTAAGCAACGGCATTGTGGTTGAAGAAGAGGATTTGATAGAGGAAGAGATCATCCGCTTCTACAAGAATCTCTATTCAAGCAAAGAGGAAGTGAGTTTCGGAATTGAAGGCCTCCATTGGAACCCAATAAGCGTAGAGAAGGCAAGATGGCTGGAGACACCATTTGAAGAAGAAGAAATTAAAAGAGCAGTGTTCGAGTGTGACACGGTTAAATCTCCGGGTCCAGACGGATTTTCATTCGCAGTTCTACAAAGAAATTGGGAAGTGGTCAAAAGAGAGGTATTAGATGTTATGGCAGAATTTCACACAAACGGAGTTGTGAATAAGGTGACGAATGAGACTTACATCTGCCTTATACCAAAGAAAGCAAATTCCCTAAAAGTTGGGGATTACAGACCTATTAGCTTGATAACAAGCTTATATAAAATTATTGCAAAGGTATTAGCTTGGAGATTGAGAGAGGTTTTAAGTGACACCATTTCTGGTGCCCAGGGCGCTTTCATAAAAGGGAGACAAATATTAGATGCAGTTTTGGTGGCTAATGAAGTGGTTGATGAGACAAGGAAGAAGAAGAAAGAGGGTCTGGTGTTCAAAATTGACTTTGAGAAAGCGTATGATCACGTAGAGTGGAACTTTTTGGATTATGCTATGGAAAGTAAAGGCTTTGGAGACAGATGGAGAAAATGGATAGGGGGTTGTCTCAGGTCAGCAAATTTTTCTATTCAGATAAATGGGAGACCAAGAGGAAAATTCGGTGCTTCTCGGGGATTAAGACAAGGTGACCCCTTATCATCTTTTCTTTTTACCCTAGTGGTGGATGTTCTGGATAGACTTATGGAGAGAGCAAGAGATTGTAGACTAATTGAAGGCCTGGTAGTCGGGAGGGAAGAGGTTGAAATCACTCATCTGCAATTCGCTGATGATACAATTTTCTTTTTGAAGGATGATGACCATAATTGGAATAATTTAAACATGGTCCTAGAAAGTTTCTGTTTGTTTTCGGGGCTGAAAATCAATAAATCAAAATGCTCCGTAGTTGGAATAAATACTGAGGCTGGGAGATTAGAAAGGATGGCTGATGATCAAGGCTGTGAGATTGGAGTTTGGCCTATGAAATACCTGGGCCTTCCCTTAGGTGGTAACCCAAAGCAAGCTTCATTTTGGAACCCAGTTGTAGAGAAAATTGAGAAGAGCTTGGAGGGTTGGAAAAAAGCTTTTTTGTCAAGAGGCGGTAGGCTAACATTGATTCAAGCAGTTCTAAGCAGCCTCCCAACATACTATTTATCTCTTTTCCAAATTCCGGTGGGAGTGGCAAAGAGATTAGAATCTCTTCTGAAAATTTTTTTCTGGGATGGAGTCGGAGAGGGTAAGAAAACCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

596

Amino Acids

68.9

Weight (kDa)

8.91

Isoelectric Point (pI)

34.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 255 - 513 1.1e-35 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000492)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01250
fragaria_vesca FvH4_1g20711 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g28252 FvH4_3g31041 FvH4_4g00611 FvH4_5g15581 FvH4_5g21911 FvH4_5g35851 FvH4_6g24331 FvH4_6g34741
malus_domestica MD07G1291800.v1.1 MD09G1222500.v1.1
prunus_persica Prupe.1G162400_v2.0.a1 Prupe.1G210400_v2.0.a1 Prupe.6G308200_v2.0.a1
pyrus_communis pycom05g02580 pycom12g09210 pycom12g15310 pycom16g09140
rosa_chinensis RchiOBHm_Chr3g0484711 RchiOBHm_Chr4g0393411 RchiOBHm_Chr4g0397521 RchiOBHm_Chr4g0404571 RchiOBHm_Chr4g0418351 RchiOBHm_Chr5g0060311 RchiOBHm_Chr6g0301391 RchiOBHm_Chr7g0229871
rosa_laevigata RLG00000007836 RLG00000025145
rosa_multiflora Rmu_co8327795.1_g000001 Rmu_sc0000469.1_g000005 Rmu_sc0000498.1_g000050 Rmu_sc0000558.1_g000007 Rmu_sc0000693.1_g000082 Rmu_sc0000711.1_g000011 Rmu_sc0000814.1_g000039 Rmu_sc0001366.1_g000005 Rmu_sc0002205.1_g000004 Rmu_sc0002283.1_g000086 Rmu_sc0002406.1_g000011 Rmu_sc0002735.1_g000022 Rmu_sc0003545.1_g000004 Rmu_sc0004406.1_g000009 Rmu_sc0006301.1_g000009 Rmu_sc0006754.1_g000004 Rmu_sc0007324.1_g000010 Rmu_sc0008199.1_g000002 Rmu_sc0008563.1_g000009 Rmu_sc0009777.1_g000007 Rmu_sc0010560.1_g000011 Rmu_sc0011095.1_g000004 Rmu_sc0012101.1_g000004 Rmu_sc0014150.1_g000009 Rmu_sc0020270.1_g000004 Rmu_sc0021327.1_g000001 Rmu_sc0031326.1_g000003 Rmu_ssc0000116.1_g000050 Rmu_ssc0000368.1_g000056
rosa_roxburghii Rroxscaffold_2G00110080 Rroxscaffold_2G00141700 Rroxscaffold_2G00142400
rosa_rugosa Rorug01G0104400 Rorug01G0204500 Rorug02G0225600 Rorug02G0262000.1 Rorug02G0526700 Rorug03G0082000 Rorug03G0102500.1 Rorug04G0106400 Rorug04G0200200 Rorug05G0021900.1 Rorug05G0240000 Rorug05G0326500 Rorug05G0529200 Rorug07G0136100
rosa_samantha Rh1AG352100 Rh6DG494100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 206
AccB1I GGYRCC 1 cut(s) 893
AccI GTMKAC 1 cut(s) 1264
AciI CCGC 2 cut(s) 463, 1638
AcsI RAATTY 6 cut(s) 707, 778, 1120, 1157, 1704, 1746
AfiI CCNNNNNNNGG 3 cut(s) 1222, 1544, 1708
AjnI CCWGG 3 cut(s) 897, 1278, 1527
AjuI GAANNNNNNNTTGG 2 cut(s) 1546, 1578
Alw26I GTCTC 9 cut(s) 131, 330, 557, 740, 913, 959, 1071, 1112, 1138
Ama87I CYCGRG 1 cut(s) 1170
AoxI GGCC 5 cut(s) 324, 517, 1276, 1514, 1531
ApeKI GCWGC 1 cut(s) 1671
ApoI RAATTY 6 cut(s) 707, 778, 1120, 1157, 1704, 1746
Asp700I GAANNNNTTC 1 cut(s) 1737
AspLEI GCGC 1 cut(s) 905
AspS9I GGNCC 4 cut(s) 324, 629, 1384, 1531
AsuC2I CCSGG 1 cut(s) 627
AsuHPI GGTGA 3 cut(s) 158, 748, 1199
AvaI CYCGRG 1 cut(s) 1170
AvaII GGWCC 2 cut(s) 629, 1384
AxyI CCTNAGG 1 cut(s) 1540
BaeGI GKGCMC 1 cut(s) 898
BanI GGYRCC 1 cut(s) 893
BbvI GCAGC 1 cut(s) 1683
BccI CCATC 5 cut(s) 59, 549, 1077, 1474, 1754
BceAI ACGGC 1 cut(s) 433
BciT130I CCWGG 3 cut(s) 899, 1280, 1529
BclI TGATCA 2 cut(s) 1024, 1489
BcnI CCSGG 1 cut(s) 627
BcoDI GTCTC 9 cut(s) 131, 330, 557, 740, 913, 959, 1071, 1112, 1138
BfaI CTAG 4 cut(s) 365, 1217, 1388, 1789
BfoI RGCGCY 1 cut(s) 906
BfuAI ACCTGC 1 cut(s) 206
BisI GCNGC 1 cut(s) 1672
BlpI GCTNAGC 1 cut(s) 312
BlsI GCNGC 1 cut(s) 1673
Bme1390I CCNGG 4 cut(s) 627, 899, 1280, 1529
Bme18I GGWCC 2 cut(s) 629, 1384
BmeT110I CYCGRG 1 cut(s) 1170
BmgT120I GGNCC 4 cut(s) 324, 629, 1384, 1531
BmiI GGNNCC 4 cut(s) 530, 630, 895, 1574
BmrFI CCNGG 4 cut(s) 627, 899, 1280, 1529
BmrI ACTGGG 1 cut(s) 1571
BmsI GCATC 1 cut(s) 922
BmuI ACTGGG 1 cut(s) 1571
BpmI CTGGAG 3 cut(s) 287, 319, 581
Bpu1102I GCTNAGC 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 627
BsaAI YACGTR 1 cut(s) 1030
BsaBI GATNNNNATC 1 cut(s) 1732
BsaI GGTCTC 1 cut(s) 1138
BsaJI CCNNGG 3 cut(s) 897, 898, 1528
BsaWI WCCGGW 1 cut(s) 1708
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 3 cut(s) 1222, 1544, 1708
Bse1I ACTGG 2 cut(s) 270, 1577
Bse21I CCTNAGG 1 cut(s) 1540
Bse8I GATNNNNATC 1 cut(s) 1732
BseBI CCWGG 3 cut(s) 899, 1280, 1529
BseDI CCNNGG 3 cut(s) 897, 898, 1528
BseGI GGATG 8 cut(s) 13, 55, 202, 459, 1231, 1357, 1485, 1765
BseJI GATNNNNATC 1 cut(s) 1732
BseLI CCNNNNNNNGG 3 cut(s) 1222, 1544, 1708
BseMII CTCAG 3 cut(s) 272, 1123, 1449
BseNI ACTGG 2 cut(s) 270, 1577
BseRI GAGGAG 1 cut(s) 284
BseSI GKGCMC 1 cut(s) 898
BseXI GCAGC 1 cut(s) 1683
BseYI CCCAGC 1 cut(s) 1463
BsgI GTGCAG 1 cut(s) 234
BshFI GGCC 5 cut(s) 326, 519, 1278, 1516, 1533
BshNI GGYRCC 1 cut(s) 893
BsiHKCI CYCGRG 1 cut(s) 1170
BsiSI CCGG 2 cut(s) 626, 1709
BslI CCNNNNNNNGG 3 cut(s) 1222, 1544, 1708
BsmAI GTCTC 9 cut(s) 131, 330, 557, 740, 913, 959, 1071, 1112, 1138
BsnI GGCC 5 cut(s) 326, 519, 1278, 1516, 1533
Bso31I GGTCTC 1 cut(s) 1138
BsoBI CYCGRG 1 cut(s) 1170
Bsp1286I GDGCHC 1 cut(s) 898
Bsp143I GATC 3 cut(s) 456, 1024, 1489
Bsp1720I GCTNAGC 1 cut(s) 312
BspACI CCGC 2 cut(s) 463, 1638
BspANI GGCC 5 cut(s) 326, 519, 1278, 1516, 1533
BspCNI CTCAG 3 cut(s) 273, 1122, 1450
BspHI TCATGA 1 cut(s) 87
BspLI GGNNCC 4 cut(s) 530, 630, 895, 1574
BspMI ACCTGC 1 cut(s) 206
BspQI GCTCTTC 1 cut(s) 1592
BspT107I GGYRCC 1 cut(s) 893
BspTNI GGTCTC 1 cut(s) 1138
BsrI ACTGG 2 cut(s) 270, 1577
BssECI CCNNGG 3 cut(s) 897, 898, 1528
BssMI GATC 3 cut(s) 456, 1024, 1489
Bst2UI CCWGG 3 cut(s) 899, 1280, 1529
Bst4CI ACNGT 2 cut(s) 62, 616
Bst6I CTCTTC 6 cut(s) 282, 429, 447, 1290, 1592, 1743
BstBAI YACGTR 1 cut(s) 1030
BstC8I GCNNGC 1 cut(s) 1560
BstDEI CTNAG 6 cut(s) 281, 312, 1109, 1458, 1540, 1667
BstEII GGTNACC 2 cut(s) 1187, 1547
BstF5I GGATG 8 cut(s) 13, 55, 202, 459, 1231, 1357, 1485, 1765
BstH2I RGCGCY 1 cut(s) 906
BstHHI GCGC 1 cut(s) 905
BstKTI GATC 3 cut(s) 459, 1027, 1492
BstMAI GTCTC 9 cut(s) 131, 330, 557, 740, 913, 959, 1071, 1112, 1138
BstMBI GATC 3 cut(s) 456, 1024, 1489
BstMWI GCNNNNNNNGC 2 cut(s) 75, 902
BstNI CCWGG 3 cut(s) 899, 1280, 1529
BstPI GGTNACC 2 cut(s) 1187, 1547
BstSCI CCNGG 4 cut(s) 625, 897, 1278, 1527
BstSLI GKGCMC 1 cut(s) 898
BstV1I GCAGC 1 cut(s) 1683
Bsu36I CCTNAGG 1 cut(s) 1540
BsuRI GGCC 5 cut(s) 326, 519, 1278, 1516, 1533
BtsCI GGATG 8 cut(s) 13, 55, 202, 459, 1231, 1357, 1485, 1765
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 1 cut(s) 603
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 1 cut(s) 1560
CciI TCATGA 1 cut(s) 87
CfoI GCGC 1 cut(s) 905
Cfr13I GGNCC 4 cut(s) 324, 629, 1384, 1531
CviAII CATG 2 cut(s) 88, 1381
DdeI CTNAG 6 cut(s) 281, 312, 1109, 1458, 1540, 1667
DpnI GATC 3 cut(s) 458, 1026, 1491
DpnII GATC 3 cut(s) 456, 1024, 1489
DraI TTTAAA 1 cut(s) 1377
Eam1104I CTCTTC 6 cut(s) 282, 429, 447, 1290, 1592, 1743
EarI CTCTTC 6 cut(s) 282, 429, 447, 1290, 1592, 1743
Eco147I AGGCCT 2 cut(s) 519, 1278
Eco31I GGTCTC 1 cut(s) 1138
Eco47I GGWCC 2 cut(s) 629, 1384
Eco81I CCTNAGG 1 cut(s) 1540
Eco88I CYCGRG 1 cut(s) 1170
Eco91I GGTNACC 2 cut(s) 1187, 1547
EcoO65I GGTNACC 2 cut(s) 1187, 1547
EcoRII CCWGG 3 cut(s) 897, 1278, 1527
FaeI CATG 2 cut(s) 91, 1384
FatI CATG 2 cut(s) 87, 1380
FbaI TGATCA 2 cut(s) 1024, 1489
FblI GTMKAC 1 cut(s) 1264
Fnu4HI GCNGC 1 cut(s) 1672
FokI GGATG 7 cut(s) 62, 209, 446, 1238, 1364, 1492, 1772
Fsp4HI GCNGC 1 cut(s) 1672
FspBI CTAG 4 cut(s) 365, 1217, 1388, 1789
GlaI GCGC 1 cut(s) 904
GluI GCNGC 1 cut(s) 1672
GsaI CCCAGC 1 cut(s) 1467
GsuI CTGGAG 3 cut(s) 287, 319, 581
HaeII RGCGCY 1 cut(s) 906
HaeIII GGCC 5 cut(s) 326, 519, 1278, 1516, 1533
HapII CCGG 2 cut(s) 626, 1709
HhaI GCGC 1 cut(s) 905
Hin1II CATG 2 cut(s) 91, 1384
Hin6I GCGC 1 cut(s) 903
HinP1I GCGC 1 cut(s) 903
HindIII AAGCTT 3 cut(s) 823, 1560, 1620
HinfI GANTC 7 cut(s) 84, 130, 351, 475, 1654, 1733, 1764
HpaII CCGG 2 cut(s) 626, 1709
HphI GGTGA 3 cut(s) 158, 748, 1199
Hpy166II GTNNAC 1 cut(s) 1265
Hpy188I TCNGA 4 cut(s) 509, 1134, 1743, 1769
Hpy188III TCNNGA 8 cut(s) 88, 100, 134, 298, 632, 1232, 1288, 1632
Hpy8I GTNNAC 1 cut(s) 1265
HpyAV CCTTC 9 cut(s) 27, 31, 47, 326, 509, 541, 1268, 1342, 1544
HpyCH4III ACNGT 2 cut(s) 62, 616
HpyCH4IV ACGT 1 cut(s) 1029
HpyCH4V TGCA 4 cut(s) 215, 842, 935, 1318
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 902
HpyF3I CTNAG 6 cut(s) 281, 312, 1109, 1458, 1540, 1667
HpySE526I ACGT 1 cut(s) 1029
Hsp92II CATG 2 cut(s) 91, 1384
HspAI GCGC 1 cut(s) 903
Ksp22I TGATCA 2 cut(s) 1024, 1489
Kzo9I GATC 3 cut(s) 456, 1024, 1489
LguI GCTCTTC 1 cut(s) 1592
LmnI GCTCC 1 cut(s) 1442
Lsp1109I GCAGC 1 cut(s) 1683
LweI GCATC 1 cut(s) 922
MaeI CTAG 4 cut(s) 365, 1217, 1388, 1789
MaeII ACGT 1 cut(s) 1029
MaeIII GTNAC 5 cut(s) 608, 736, 878, 1187, 1547
MalI GATC 3 cut(s) 458, 1026, 1491
MboI GATC 3 cut(s) 456, 1024, 1489
MhlI GDGCHC 1 cut(s) 898
MlyI GAGTC 2 cut(s) 139, 1773
MmeI TCCRAC 3 cut(s) 1426, 1592, 1747
MroXI GAANNNNTTC 1 cut(s) 1737
MseI TTAA 7 cut(s) 119, 410, 588, 618, 875, 1178, 1376
MspI CCGG 2 cut(s) 626, 1709
MspR9I CCNGG 4 cut(s) 627, 899, 1280, 1529
MvaI CCWGG 3 cut(s) 899, 1280, 1529
MwoI GCNNNNNNNGC 2 cut(s) 75, 902
NciI CCSGG 1 cut(s) 627
NdeII GATC 3 cut(s) 456, 1024, 1489
NlaIII CATG 2 cut(s) 91, 1384
NlaIV GGNNCC 4 cut(s) 530, 630, 895, 1574
NmuCI GTSAC 4 cut(s) 608, 736, 878, 1187
PagI TCATGA 1 cut(s) 87
PasI CCCWGGG 1 cut(s) 898
PceI AGGCCT 2 cut(s) 519, 1278
PciSI GCTCTTC 1 cut(s) 1592
PdmI GAANNNNTTC 1 cut(s) 1737
PfeI GAWTC 5 cut(s) 84, 351, 475, 1654, 1733
PkrI GCNGC 1 cut(s) 1673
PleI GAGTC 2 cut(s) 138, 1772
PpsI GAGTC 2 cut(s) 138, 1772
Ppu21I YACGTR 1 cut(s) 1030
Psp6I CCWGG 3 cut(s) 897, 1278, 1527
PspEI GGTNACC 2 cut(s) 1187, 1547
PspFI CCCAGC 1 cut(s) 1463
PspGI CCWGG 3 cut(s) 897, 1278, 1527
PspN4I GGNNCC 4 cut(s) 530, 630, 895, 1574
PspPI GGNCC 4 cut(s) 324, 629, 1384, 1531
SapI GCTCTTC 1 cut(s) 1592
SaqAI TTAA 7 cut(s) 119, 410, 588, 618, 875, 1178, 1376
SatI GCNGC 1 cut(s) 1672
Sau3AI GATC 3 cut(s) 456, 1024, 1489
Sau96I GGNCC 4 cut(s) 324, 629, 1384, 1531
SchI GAGTC 2 cut(s) 139, 1773
ScrFI CCNGG 4 cut(s) 627, 899, 1280, 1529
SduI GDGCHC 1 cut(s) 898
SfaNI GCATC 1 cut(s) 922
SinI GGWCC 2 cut(s) 629, 1384
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
SseBI AGGCCT 2 cut(s) 519, 1278
SsiI CCGC 2 cut(s) 463, 1638
SspI AATATT 1 cut(s) 927
SspMI CTAG 4 cut(s) 365, 1217, 1388, 1789
StuI AGGCCT 2 cut(s) 519, 1278
StyD4I CCNGG 4 cut(s) 625, 897, 1278, 1527
TaaI ACNGT 2 cut(s) 62, 616
TaiI ACGT 1 cut(s) 1032
TaqI TCGA 2 cut(s) 133, 603
TfiI GAWTC 5 cut(s) 84, 351, 475, 1654, 1733
Tru1I TTAA 7 cut(s) 119, 410, 588, 618, 875, 1178, 1376
Tru9I TTAA 7 cut(s) 119, 410, 588, 618, 875, 1178, 1376
TscAI CASTG 1 cut(s) 603
TseFI GTSAC 4 cut(s) 608, 736, 878, 1187
TseI GCWGC 1 cut(s) 1671
Tsp45I GTSAC 4 cut(s) 608, 736, 878, 1187
TspDTI ATGAA 7 cut(s) 66, 76, 633, 898, 966, 1535, 1554
TspGWI ACGGA 3 cut(s) 651, 735, 1429
TspRI CASTG 1 cut(s) 603
VpaK11BI GGWCC 2 cut(s) 629, 1384
XapI RAATTY 6 cut(s) 707, 778, 1120, 1157, 1704, 1746
XmiI GTMKAC 1 cut(s) 1264
XmnI GAANNNNTTC 1 cut(s) 1737
XspI CTAG 4 cut(s) 365, 1217, 1388, 1789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.