Rmu_sc0020270.1_g000004

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020270.1
OG0000492
Physical Location & Seq
Forward (+)
26726 .. 28174
1449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020270.1_g000004.1.cds

Sequence Viewer

Length: 723 bp
atgccaaaccatgctatgtcgtgttttaaatgctccaagaagataactaacaatattgacaagcaggccagatcctttttatggggatcagacatgacatgtcccccaattgcttggaaacaggtctgccaacccaaatcaatggggggtctagctataaggctgtctgctttcttcaacaatgcagctatagcaaaattggcgtggaagataatcaaagacccaaataactggtgggctcaaataatgagacaaaagtacttaaggaatgcctcttttttccaggccaagaagaagcagtcaaactctcttgtgtggaaaggagtactgaatgcacgagacctcattcttaaaggcataagatggatcttaggcaatgggaaatcaatccatatctggatatttaattgggtgtttgatcaccccttgcacatttatgttgcagaagacaaaaggcacctaatcaactgggatgaaactgttgacaactatatacaggacaaggcctggaacaaaggtggttttgttatacgtgatgttcatggcaagcccctggttgcagcaactgaacaagttggtacttctacagttcctttagcagaagccattgctttacgtaacagactcgtctgtgctttagaacgaggacacaacaaaattgaagttgagggagactccaaacttgtaatagatgctgtcaataaagttatacacccaccctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

240

Amino Acids

27.35

Weight (kDa)

9.77

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000492)

Species Orthologous Gene IDs
arabidopsis_thaliana ATMG01250
fragaria_vesca FvH4_1g20711 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g24611 FvH4_3g28252 FvH4_3g31041 FvH4_4g00611 FvH4_5g15581 FvH4_5g21911 FvH4_5g35851 FvH4_6g24331 FvH4_6g34741
malus_domestica MD07G1291800.v1.1 MD09G1222500.v1.1
prunus_persica Prupe.1G162400_v2.0.a1 Prupe.1G210400_v2.0.a1 Prupe.6G308200_v2.0.a1
pyrus_communis pycom05g02580 pycom12g09210 pycom12g15310 pycom16g09140
rosa_chinensis RchiOBHm_Chr3g0484711 RchiOBHm_Chr4g0393411 RchiOBHm_Chr4g0397521 RchiOBHm_Chr4g0404571 RchiOBHm_Chr4g0418351 RchiOBHm_Chr5g0060311 RchiOBHm_Chr6g0301391 RchiOBHm_Chr7g0229871
rosa_laevigata RLG00000007836 RLG00000025145
rosa_multiflora Rmu_co8327795.1_g000001 Rmu_sc0000469.1_g000005 Rmu_sc0000498.1_g000050 Rmu_sc0000558.1_g000007 Rmu_sc0000693.1_g000082 Rmu_sc0000711.1_g000011 Rmu_sc0000814.1_g000039 Rmu_sc0001366.1_g000005 Rmu_sc0002205.1_g000004 Rmu_sc0002283.1_g000086 Rmu_sc0002406.1_g000011 Rmu_sc0002735.1_g000022 Rmu_sc0003545.1_g000004 Rmu_sc0004406.1_g000009 Rmu_sc0006301.1_g000009 Rmu_sc0006754.1_g000004 Rmu_sc0007324.1_g000010 Rmu_sc0008199.1_g000002 Rmu_sc0008563.1_g000009 Rmu_sc0009777.1_g000007 Rmu_sc0010560.1_g000011 Rmu_sc0011095.1_g000004 Rmu_sc0012101.1_g000004 Rmu_sc0014150.1_g000009 Rmu_sc0020270.1_g000004 Rmu_sc0021327.1_g000001 Rmu_sc0031326.1_g000003 Rmu_ssc0000116.1_g000050 Rmu_ssc0000368.1_g000056
rosa_roxburghii Rroxscaffold_2G00110080 Rroxscaffold_2G00141700 Rroxscaffold_2G00142400
rosa_rugosa Rorug01G0104400 Rorug01G0204500 Rorug02G0225600 Rorug02G0262000.1 Rorug02G0526700 Rorug03G0082000 Rorug03G0102500.1 Rorug04G0106400 Rorug04G0200200 Rorug05G0021900.1 Rorug05G0240000 Rorug05G0326500 Rorug05G0529200 Rorug07G0136100
rosa_samantha Rh1AG352100 Rh6DG494100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 456
AclWI GGATC 3 cut(s) 66, 94, 374
AfaI GTAC 3 cut(s) 260, 327, 580
AfiI CCNNNNNNNGG 1 cut(s) 81
AflII CTTAAG 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 98
AgsI TTSAA 2 cut(s) 178, 662
AjnI CCWGG 3 cut(s) 282, 506, 552
AluBI AGCT 2 cut(s) 155, 188
AluI AGCT 2 cut(s) 155, 188
Alw26I GTCTC 3 cut(s) 244, 333, 666
AlwI GGATC 3 cut(s) 66, 94, 374
AlwNI CAGNNNCTG 1 cut(s) 566
AoxI GGCC 3 cut(s) 66, 285, 504
ApeKI GCWGC 2 cut(s) 185, 560
AsuHPI GGTGA 1 cut(s) 413
BanI GGYRCC 1 cut(s) 456
BanII GRGCYC 1 cut(s) 241
BauI CACGAG 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 197, 572
BccI CCATC 1 cut(s) 357
BciT130I CCWGG 3 cut(s) 284, 508, 554
BclI TGATCA 1 cut(s) 418
BcoDI GTCTC 3 cut(s) 244, 333, 666
BfaI CTAG 2 cut(s) 152, 721
BfmI CTRYAG 2 cut(s) 189, 585
BfrI CTTAAG 1 cut(s) 262
BisI GCNGC 2 cut(s) 186, 561
BlsI GCNGC 2 cut(s) 187, 562
BmcAI AGTACT 2 cut(s) 260, 327
Bme1390I CCNGG 3 cut(s) 284, 508, 554
BmiI GGNNCC 1 cut(s) 458
BmrFI CCNGG 3 cut(s) 284, 508, 554
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 1 cut(s) 682
BmuI ACTGGG 1 cut(s) 478
BpiI GAAGAC 1 cut(s) 453
BplI GAGNNNNNCTC 2 cut(s) 659, 691
BsaAI YACGTR 2 cut(s) 533, 617
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 1 cut(s) 552
Bsc4I CCNNNNNNNGG 1 cut(s) 81
Bse1I ACTGG 2 cut(s) 236, 473
Bse3DI GCAATG 2 cut(s) 382, 606
BseBI CCWGG 3 cut(s) 284, 508, 554
BseDI CCNNGG 1 cut(s) 552
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 1 cut(s) 81
BseMI GCAATG 2 cut(s) 382, 606
BseNI ACTGG 2 cut(s) 236, 473
BseXI GCAGC 2 cut(s) 197, 572
BshFI GGCC 3 cut(s) 68, 287, 506
BshNI GGYRCC 1 cut(s) 456
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 81
BsmAI GTCTC 3 cut(s) 244, 333, 666
BsmFI GGGAC 1 cut(s) 87
BsmI GAATGC 2 cut(s) 274, 337
BsnI GGCC 3 cut(s) 68, 287, 506
Bso31I GGTCTC 1 cut(s) 333
Bsp1286I GDGCHC 1 cut(s) 241
Bsp143I GATC 4 cut(s) 71, 86, 366, 418
BspANI GGCC 3 cut(s) 68, 287, 506
BspLI GGNNCC 1 cut(s) 458
BspPI GGATC 3 cut(s) 66, 94, 374
BspT107I GGYRCC 1 cut(s) 456
BspTI CTTAAG 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 333
BsrDI GCAATG 2 cut(s) 382, 606
BsrI ACTGG 2 cut(s) 236, 473
BssECI CCNNGG 1 cut(s) 552
BssMI GATC 4 cut(s) 71, 86, 366, 418
BssSI CACGAG 1 cut(s) 336
Bst2BI CACGAG 1 cut(s) 336
Bst2UI CCWGG 3 cut(s) 284, 508, 554
Bst4CI ACNGT 2 cut(s) 481, 589
BstAFI CTTAAG 1 cut(s) 262
BstBAI YACGTR 2 cut(s) 533, 617
BstC8I GCNNGC 2 cut(s) 66, 548
BstDEI CTNAG 1 cut(s) 370
BstF5I GGATG 1 cut(s) 478
BstKTI GATC 4 cut(s) 74, 89, 369, 421
BstMAI GTCTC 3 cut(s) 244, 333, 666
BstMBI GATC 4 cut(s) 71, 86, 366, 418
BstMWI GCNNNNNNNGC 2 cut(s) 191, 200
BstNI CCWGG 3 cut(s) 284, 508, 554
BstNSI RCATGY 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 282, 506, 552
BstSFI CTRYAG 2 cut(s) 189, 585
BstSNI TACGTA 1 cut(s) 617
BstV1I GCAGC 2 cut(s) 197, 572
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 2 cut(s) 71, 366
BstXI CCANNNNNNTGG 3 cut(s) 114, 142, 231
BstYI RGATCY 2 cut(s) 71, 366
BsuRI GGCC 3 cut(s) 68, 287, 506
BtsCI GGATG 1 cut(s) 478
Cac8I GCNNGC 2 cut(s) 66, 548
CaiI CAGNNNCTG 1 cut(s) 566
Csp6I GTAC 3 cut(s) 259, 326, 579
CviAII CATG 4 cut(s) 11, 94, 99, 542
CviJI RGCY 9 cut(s) 68, 155, 163, 188, 239, 287, 506, 550, 605
CviKI_1 RGCY 9 cut(s) 68, 155, 163, 188, 239, 287, 506, 550, 605
CviQI GTAC 3 cut(s) 259, 326, 579
DdeI CTNAG 1 cut(s) 370
DpnI GATC 4 cut(s) 73, 88, 368, 420
DpnII GATC 4 cut(s) 71, 86, 366, 418
DraI TTTAAA 1 cut(s) 28
Eco105I TACGTA 1 cut(s) 617
Eco147I AGGCCT 1 cut(s) 506
Eco24I GRGCYC 1 cut(s) 241
Eco31I GGTCTC 1 cut(s) 333
EcoRII CCWGG 3 cut(s) 282, 506, 552
EcoT38I GRGCYC 1 cut(s) 241
FaeI CATG 4 cut(s) 14, 97, 102, 545
FaqI GGGAC 1 cut(s) 87
FatI CATG 4 cut(s) 10, 93, 98, 541
FbaI TGATCA 1 cut(s) 418
Fnu4HI GCNGC 2 cut(s) 186, 561
FokI GGATG 1 cut(s) 485
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 2 cut(s) 186, 561
FspBI CTAG 2 cut(s) 152, 721
GluI GCNGC 2 cut(s) 186, 561
HaeIII GGCC 3 cut(s) 68, 287, 506
Hin1II CATG 4 cut(s) 14, 97, 102, 545
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 2 cut(s) 624, 674
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 1 cut(s) 397
Hpy8I GTNNAC 1 cut(s) 484
HpyCH4III ACNGT 2 cut(s) 481, 589
HpyCH4IV ACGT 2 cut(s) 532, 616
HpyCH4V TGCA 5 cut(s) 185, 335, 430, 443, 560
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 200
HpyF3I CTNAG 1 cut(s) 370
HpySE526I ACGT 2 cut(s) 532, 616
Hsp92II CATG 4 cut(s) 14, 97, 102, 545
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 4 cut(s) 71, 86, 366, 418
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 2 cut(s) 197, 572
LweI GCATC 1 cut(s) 682
MaeI CTAG 2 cut(s) 152, 721
MaeII ACGT 2 cut(s) 532, 616
MaeIII GTNAC 1 cut(s) 617
MalI GATC 4 cut(s) 73, 88, 368, 420
MboI GATC 4 cut(s) 71, 86, 366, 418
MboII GAAGA 5 cut(s) 52, 166, 220, 304, 458
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 2 cut(s) 71, 366
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 4 cut(s) 108, 197, 406, 657
MlyI GAGTC 2 cut(s) 618, 668
MnlI CCTC 4 cut(s) 283, 353, 638, 661
MseI TTAA 4 cut(s) 27, 263, 351, 405
MslI CAYNNNNRTG 1 cut(s) 435
MspCI CTTAAG 1 cut(s) 262
MspR9I CCNGG 3 cut(s) 284, 508, 554
MunI CAATTG 1 cut(s) 108
Mva1269I GAATGC 2 cut(s) 274, 337
MvaI CCWGG 3 cut(s) 284, 508, 554
MwoI GCNNNNNNNGC 2 cut(s) 191, 200
NdeII GATC 4 cut(s) 71, 86, 366, 418
NlaIII CATG 4 cut(s) 14, 97, 102, 545
NlaIV GGNNCC 1 cut(s) 458
NspI RCATGY 1 cut(s) 102
PceI AGGCCT 1 cut(s) 506
PciI ACATGT 1 cut(s) 98
PctI GAATGC 2 cut(s) 274, 337
PkrI GCNGC 2 cut(s) 187, 562
PleI GAGTC 2 cut(s) 618, 668
PpsI GAGTC 2 cut(s) 618, 668
Ppu21I YACGTR 2 cut(s) 533, 617
PscI ACATGT 1 cut(s) 98
Psp6I CCWGG 3 cut(s) 282, 506, 552
PspGI CCWGG 3 cut(s) 282, 506, 552
PspN4I GGNNCC 1 cut(s) 458
PstNI CAGNNNCTG 1 cut(s) 566
PsuI RGATCY 2 cut(s) 71, 366
RsaI GTAC 3 cut(s) 260, 327, 580
RsaNI GTAC 3 cut(s) 259, 326, 579
RseI CAYNNNNRTG 1 cut(s) 435
SaqAI TTAA 4 cut(s) 27, 263, 351, 405
SatI GCNGC 2 cut(s) 186, 561
Sau3AI GATC 4 cut(s) 71, 86, 366, 418
ScaI AGTACT 2 cut(s) 260, 327
SchI GAGTC 2 cut(s) 618, 668
ScrFI CCNGG 3 cut(s) 284, 508, 554
SduI GDGCHC 1 cut(s) 241
SetI ASST 8 cut(s) 126, 157, 190, 345, 462, 520, 535, 619
SfaNI GCATC 1 cut(s) 682
SfcI CTRYAG 2 cut(s) 189, 585
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 1 cut(s) 262
SmoI CTYRAG 1 cut(s) 262
SnaBI TACGTA 1 cut(s) 617
Sse9I AATT 4 cut(s) 108, 197, 406, 657
SseBI AGGCCT 1 cut(s) 506
SspI AATATT 1 cut(s) 55
SspMI CTAG 2 cut(s) 152, 721
StuI AGGCCT 1 cut(s) 506
StyD4I CCNGG 3 cut(s) 282, 506, 552
TaaI ACNGT 2 cut(s) 481, 589
TaiI ACGT 2 cut(s) 535, 619
TasI AATT 4 cut(s) 108, 197, 406, 657
TatI WGTACW 2 cut(s) 258, 325
Tru1I TTAA 4 cut(s) 27, 263, 351, 405
Tru9I TTAA 4 cut(s) 27, 263, 351, 405
TseI GCWGC 2 cut(s) 185, 560
TspDTI ATGAA 2 cut(s) 489, 530
Vha464I CTTAAG 1 cut(s) 262
XceI RCATGY 1 cut(s) 102
XcmI CCANNNNNNNNNTGG 1 cut(s) 231
XspI CTAG 2 cut(s) 152, 721
ZrmI AGTACT 2 cut(s) 260, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.