FvH4_6g30300

C-5 cytosine-specific DNA methylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000338
Physical Location & Seq
Reverse (-)
23377219 .. 23381485
4267 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30300.t1

Sequence Viewer

Length: 1734 bp
ATGGATGAGAGTCATTCTAGTGTGGATGACAATGATACTGACTGGAACACAGATGACGAAATCGACAACTTCACTCTCTTGCCTTCTTCGGGTGTGATACATCCTTGTCCACAAGCTAATGTGCTCTCCTGGGAGGTGAGCTCATCACGTCCAAGCTCTTCCAACTCCAAAGTTCTTGATCAGTTTATAGGGATGGGATTTTCAAAGAAGATGGTTGAAAAAGCAATTGAAGAACATGGAGAAGAAAATACTGAGGCAGTTCTGGAAACGCTCCTCACCTATTCGGCTCTTGAAAGTTCACCTCATGAACAGCTACAAATGGATTCTGATCTCTTCTCCTCGGATGACGAAAGCTTTTTTGATGATTTTTCAGATGAGGATATTACAAGTCCTGCATCTGAGGAGGAAAATCTACTCATGTCTTTGATCACAATGGGGTACCAAAAAGAGGAGGCTACGACAGCAATAGAGAGATGTGGTCCTAGCTCCTCACTTGTGGAGTTGACTGATTTCTTATCTGCTGCTCAGTTGGTAAAGGAAGAAGATCTGGATGCCCTTTTTTCTCTTGAAGAGACGCCTAGACTAGAACATGTGCACACTCATGATCCTAAGAAGAAACGGAAAGTTACTGTATCTGATATATTGAAAAGGAAAAGGCAGAGGGGGCACATATGCACAGATGATGAGACACTTCGCCTCCCAAATCCGATGATTGGGTTTGGAATTCCAACTGGAGCATGCCAATCTAGGAGAATTCTTCCAAATGCAGCCATAGGGCCTCCATACTTTTATTACGAGAATGTTGCCCTTGCTCCTAAAGGGGTTTGGGAGACTATATCCAGATCTTTATACGATGTTCCACCAGAGTTTGTTGATTCAAAACATTTCTGTTGTGCAGCTAGAAAGAGGGGTTACGTCCACAATCTTCCAATTGCGAACAGATTTCCTCTAACTCCACTCCCCCCACAAACCATACATGAGGCATTTCCCTTGACAAAAGAATGGTGGCCTTCTTGGGACATAAGAACAAAGCTAAACTGTATAGTAACTTGCTATTCTAGTGCAAAGTTATCAGAGAGGATGCTGAAGCTTCTTCAGGGCTGTGATGGCATTCCACCAGAAAGAGTCCAGCGGGCTATCATGCATGAATGCCGCAAATGGAATCTATTGTGGGTTGGAAAAAATAAAGTTGCCCCACTTGAGCCTGGTGAAGTGGAAATCCTCATGGGGTTTCCAAAGGATCACACAAGAGGAGTCAGCCGCACTGATAGATACAAATCAATTGGCAATGCATTTCAGGTTCACACTGTTGCATACCACTTCTCGGTCTTGCGAGACAAGTTTCCCAATGGTATCAACGTCCTCTCTCTATTCTCTGGTATAGGTGGAGCAGAAGTAGCCCTTCATAGTCTAGGGATCCGTATGAAGAATGTGGTCTCTGTAGAGATATCAGAAGTGAACAGAGACATAGTGCAGTGTTGGTGGGAGCAAACAGAGCAAACTGGGAAATTGATTCACCTTGCTGATGTAAAAGAGCTGGATTCTGAGCGTCTGAAACAGTTTATCCATTCATTCGGAGGATTTGATCTTGTAGTGGGTGGGAGCCCCTGCAATAATCTAACCGGCAGCAACAGGTATCATCGAGATGGACTCGAGGGCAAAGAATCTTCACTTTTCTTTCACTACTGTCGAATTCTGGACTTGGTCAAGTCTATTATGGCAGGACTATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

578

Amino Acids

64.98

Weight (kDa)

5.31

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 453 - 567 8.9e-10 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 438
AccB1I GGYRCC 1 cut(s) 438
AciI CCGC 3 cut(s) 1132, 1153, 1261
AclWI GGATC 4 cut(s) 599, 1248, 1411, 1424
AcsI RAATTY 3 cut(s) 723, 753, 1692
AcuI CTGAAG 2 cut(s) 1079, 1106
AcyI GRCGYC 1 cut(s) 575
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 4 cut(s) 89, 448, 713, 1324
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 7 cut(s) 204, 218, 230, 293, 569, 646, 879
AjiI CACGTC 1 cut(s) 149
AjnI CCWGG 2 cut(s) 128, 1204
Alw21I GWGCWC 3 cut(s) 126, 143, 597
Alw26I GTCTC 6 cut(s) 566, 680, 824, 1329, 1441, 1458
Alw44I GTGCAC 1 cut(s) 593
AlwI GGATC 4 cut(s) 599, 1248, 1411, 1424
Ama87I CYCGRG 1 cut(s) 1652
AoxI GGCC 2 cut(s) 776, 1007
ApaLI GTGCAC 1 cut(s) 593
ApeKI GCWGC 4 cut(s) 521, 767, 896, 1626
ApoI RAATTY 3 cut(s) 723, 753, 1692
Asp718I GGTACC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 479, 776
AsuHPI GGTGA 5 cut(s) 148, 268, 291, 1220, 1508
AvaI CYCGRG 1 cut(s) 1652
AvaII GGWCC 1 cut(s) 479
BaeGI GKGCMC 2 cut(s) 597, 669
BamHI GGATCC 1 cut(s) 1416
BanI GGYRCC 1 cut(s) 438
BanII GRGCYC 2 cut(s) 143, 1607
Bbv12I GWGCWC 3 cut(s) 126, 143, 597
BbvI GCAGC 4 cut(s) 508, 779, 908, 1638
BccI CCATC 4 cut(s) 187, 205, 1100, 1640
BcgI CGANNNNNNTGC 2 cut(s) 785, 819
BciT130I CCWGG 2 cut(s) 130, 1206
BclI TGATCA 2 cut(s) 178, 426
BcoDI GTCTC 6 cut(s) 566, 680, 824, 1329, 1441, 1458
BfaI CTAG 8 cut(s) 18, 483, 579, 584, 747, 900, 1059, 1412
BfmI CTRYAG 1 cut(s) 1440
BglII AGATCT 2 cut(s) 544, 842
BisI GCNGC 6 cut(s) 522, 768, 897, 1153, 1261, 1627
BlsI GCNGC 6 cut(s) 523, 769, 898, 1154, 1262, 1628
Bme1390I CCNGG 2 cut(s) 130, 1206
Bme18I GGWCC 1 cut(s) 479
BmeT110I CYCGRG 1 cut(s) 1652
BmgBI CACGTC 1 cut(s) 149
BmgT120I GGNCC 2 cut(s) 479, 776
BmiI GGNNCC 3 cut(s) 440, 1418, 1604
BmrFI CCNGG 2 cut(s) 130, 1206
BmrI ACTGGG 1 cut(s) 1512
BmsI GCATC 3 cut(s) 404, 541, 1071
BmuI ACTGGG 1 cut(s) 1512
BplI GAGNNNNNCTC 4 cut(s) 125, 157, 1635, 1667
BpmI CTGGAG 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 1220
BsaBI GATNNNNATC 2 cut(s) 327, 1276
BsaHI GRCGYC 1 cut(s) 575
BsaI GGTCTC 1 cut(s) 1441
BsaJI CCNNGG 2 cut(s) 129, 339
BsaXI ACNNNNNCTCC 4 cut(s) 681, 711, 1380, 1410
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 448, 713, 1324
Bse118I RCCGGY 1 cut(s) 1622
Bse1I ACTGG 3 cut(s) 47, 736, 1507
Bse3DI GCAATG 1 cut(s) 1294
Bse8I GATNNNNATC 2 cut(s) 327, 1276
BseBI CCWGG 2 cut(s) 130, 1206
BseDI CCNNGG 2 cut(s) 129, 339
BseGI GGATG 7 cut(s) 10, 31, 100, 198, 349, 556, 1086
BseJI GATNNNNATC 2 cut(s) 327, 1276
BseLI CCNNNNNNNGG 4 cut(s) 89, 448, 713, 1324
BseMI GCAATG 1 cut(s) 1294
BseMII CTCAG 4 cut(s) 243, 390, 539, 1536
BseNI ACTGG 3 cut(s) 47, 736, 1507
BseRI GAGGAG 6 cut(s) 263, 328, 416, 464, 478, 1266
BseSI GKGCMC 2 cut(s) 597, 669
BseXI GCAGC 4 cut(s) 508, 779, 908, 1638
BsgI GTGCAG 2 cut(s) 915, 1493
BshFI GGCC 2 cut(s) 778, 1009
BshNI GGYRCC 1 cut(s) 438
BsiHKAI GWGCWC 3 cut(s) 126, 143, 597
BsiHKCI CYCGRG 1 cut(s) 1652
BsiSI CCGG 1 cut(s) 1623
BslFI GGGAC 1 cut(s) 1031
BslI CCNNNNNNNGG 4 cut(s) 89, 448, 713, 1324
BsmAI GTCTC 6 cut(s) 566, 680, 824, 1329, 1441, 1458
BsmBI CGTCTC 1 cut(s) 566
BsmFI GGGAC 1 cut(s) 1031
BsmI GAATGC 2 cut(s) 1110, 1154
BsnI GGCC 2 cut(s) 778, 1009
Bso31I GGTCTC 1 cut(s) 1441
BsoBI CYCGRG 1 cut(s) 1652
Bsp1286I GDGCHC 5 cut(s) 126, 143, 597, 669, 1607
Bsp143I GATC 9 cut(s) 178, 328, 426, 544, 604, 842, 1240, 1416, 1585
BspACI CCGC 3 cut(s) 1132, 1153, 1261
BspANI GGCC 2 cut(s) 778, 1009
BspCNI CTCAG 4 cut(s) 244, 391, 538, 1537
BspHI TCATGA 2 cut(s) 304, 601
BspLI GGNNCC 3 cut(s) 440, 1418, 1604
BspPI GGATC 4 cut(s) 599, 1248, 1411, 1424
BspQI GCTCTTC 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 1441
BsrDI GCAATG 1 cut(s) 1294
BsrFI RCCGGY 1 cut(s) 1622
BsrI ACTGG 3 cut(s) 47, 736, 1507
BssAI RCCGGY 1 cut(s) 1622
BssECI CCNNGG 2 cut(s) 129, 339
BssMI GATC 9 cut(s) 178, 328, 426, 544, 604, 842, 1240, 1416, 1585
BssNI GRCGYC 1 cut(s) 575
Bst2UI CCWGG 2 cut(s) 130, 1206
Bst4CI ACNGT 5 cut(s) 631, 1040, 1309, 1560, 1688
Bst6I CTCTTC 3 cut(s) 163, 338, 564
BstACI GRCGYC 1 cut(s) 575
BstC8I GCNNGC 2 cut(s) 739, 1134
BstDEI CTNAG 5 cut(s) 252, 399, 525, 609, 1545
BstF5I GGATG 7 cut(s) 10, 31, 100, 198, 349, 556, 1086
BstKTI GATC 9 cut(s) 181, 331, 429, 547, 607, 845, 1243, 1419, 1588
BstMAI GTCTC 6 cut(s) 566, 680, 824, 1329, 1441, 1458
BstMBI GATC 9 cut(s) 178, 328, 426, 544, 604, 842, 1240, 1416, 1585
BstMWI GCNNNNNNNGC 5 cut(s) 461, 664, 1107, 1397, 1495
BstNI CCWGG 2 cut(s) 130, 1206
BstNSI RCATGY 2 cut(s) 593, 741
BstSCI CCNGG 2 cut(s) 128, 1204
BstSFI CTRYAG 1 cut(s) 1440
BstSLI GKGCMC 2 cut(s) 597, 669
BstV1I GCAGC 4 cut(s) 508, 779, 908, 1638
BstX2I RGATCY 3 cut(s) 544, 842, 1416
BstYI RGATCY 3 cut(s) 544, 842, 1416
BsuRI GGCC 2 cut(s) 778, 1009
BtrI CACGTC 1 cut(s) 149
BtsCI GGATG 7 cut(s) 10, 31, 100, 198, 349, 556, 1086
BtsI GCAGTG 1 cut(s) 1481
BtsIMutI CAGTG 3 cut(s) 1263, 1305, 1481
Cac8I GCNNGC 2 cut(s) 739, 1134
CciI TCATGA 2 cut(s) 304, 601
Cfr10I RCCGGY 1 cut(s) 1622
Cfr13I GGNCC 2 cut(s) 479, 776
CseI GACGC 2 cut(s) 583, 1538
Csp6I GTAC 1 cut(s) 439
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 5 cut(s) 252, 399, 525, 609, 1545
DpnI GATC 9 cut(s) 180, 330, 428, 546, 606, 844, 1242, 1418, 1587
DpnII GATC 9 cut(s) 178, 328, 426, 544, 604, 842, 1240, 1416, 1585
Eam1104I CTCTTC 3 cut(s) 163, 338, 564
EarI CTCTTC 3 cut(s) 163, 338, 564
Ecl136II GAGCTC 1 cut(s) 141
Eco24I GRGCYC 2 cut(s) 143, 1607
Eco31I GGTCTC 1 cut(s) 1441
Eco32I GATATC 1 cut(s) 1449
Eco47I GGWCC 1 cut(s) 479
Eco53kI GAGCTC 1 cut(s) 141
Eco57I CTGAAG 2 cut(s) 1079, 1106
Eco88I CYCGRG 1 cut(s) 1652
EcoICRI GAGCTC 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 776
EcoRI GAATTC 3 cut(s) 723, 753, 1692
EcoRII CCWGG 2 cut(s) 128, 1204
EcoRV GATATC 1 cut(s) 1449
EcoT22I ATGCAT 2 cut(s) 1146, 1294
EcoT38I GRGCYC 2 cut(s) 143, 1607
Esp3I CGTCTC 1 cut(s) 566
FalI AAGNNNNNCTT 2 cut(s) 1386, 1418
FaqI GGGAC 1 cut(s) 1031
FauI CCCGC 1 cut(s) 1125
FauNDI CATATG 1 cut(s) 671
FbaI TGATCA 2 cut(s) 178, 426
Fnu4HI GCNGC 6 cut(s) 522, 768, 897, 1153, 1261, 1627
FokI GGATG 7 cut(s) 17, 38, 87, 205, 356, 563, 1093
FriOI GRGCYC 2 cut(s) 143, 1607
Fsp4HI GCNGC 6 cut(s) 522, 768, 897, 1153, 1261, 1627
FspBI CTAG 8 cut(s) 18, 483, 579, 584, 747, 900, 1059, 1412
GluI GCNGC 6 cut(s) 522, 768, 897, 1153, 1261, 1627
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 2 cut(s) 778, 1009
HapII CCGG 1 cut(s) 1623
HgaI GACGC 2 cut(s) 583, 1538
Hin1I GRCGYC 1 cut(s) 575
HincII GTYRAC 1 cut(s) 504
HindII GTYRAC 1 cut(s) 504
HindIII AAGCTT 2 cut(s) 352, 1088
HpaII CCGG 1 cut(s) 1623
HphI GGTGA 5 cut(s) 148, 268, 291, 1220, 1508
Hpy166II GTNNAC 7 cut(s) 110, 299, 504, 595, 919, 1303, 1459
Hpy8I GTNNAC 7 cut(s) 110, 299, 504, 595, 919, 1303, 1459
HpyAV CCTTC 3 cut(s) 93, 1020, 1412
HpyCH4III ACNGT 5 cut(s) 631, 1040, 1309, 1560, 1688
HpyCH4IV ACGT 3 cut(s) 148, 915, 1359
HpyF10VI GCNNNNNNNGC 5 cut(s) 461, 664, 1107, 1397, 1495
HpyF3I CTNAG 5 cut(s) 252, 399, 525, 609, 1545
HpySE526I ACGT 3 cut(s) 148, 915, 1359
Hsp92I GRCGYC 1 cut(s) 575
KpnI GGTACC 1 cut(s) 442
Ksp22I TGATCA 2 cut(s) 178, 426
Kzo9I GATC 9 cut(s) 178, 328, 426, 544, 604, 842, 1240, 1416, 1585
LguI GCTCTTC 1 cut(s) 163
LmnI GCTCC 7 cut(s) 276, 491, 734, 817, 1388, 1486, 1602
Lsp1109I GCAGC 4 cut(s) 508, 779, 908, 1638
LweI GCATC 3 cut(s) 404, 541, 1071
MaeI CTAG 8 cut(s) 18, 483, 579, 584, 747, 900, 1059, 1412
MaeII ACGT 3 cut(s) 148, 915, 1359
MaeIII GTNAC 3 cut(s) 625, 911, 1045
MalI GATC 9 cut(s) 180, 330, 428, 546, 606, 844, 1242, 1418, 1587
MboI GATC 9 cut(s) 178, 328, 426, 544, 604, 842, 1240, 1416, 1585
MfeI CAATTG 3 cut(s) 225, 930, 1281
MflI RGATCY 3 cut(s) 544, 842, 1416
MhlI GDGCHC 5 cut(s) 126, 143, 597, 669, 1607
MluCI AATT 7 cut(s) 225, 723, 753, 930, 1281, 1508, 1692
MlyI GAGTC 4 cut(s) 19, 1134, 1263, 1644
MmeI TCCRAC 3 cut(s) 186, 752, 1156
Mph1103I ATGCAT 2 cut(s) 1146, 1294
MslI CAYNNNNRTG 3 cut(s) 18, 600, 1644
MspA1I CMGCKG 1 cut(s) 1132
MspI CCGG 1 cut(s) 1623
MspR9I CCNGG 2 cut(s) 130, 1206
MunI CAATTG 3 cut(s) 225, 930, 1281
Mva1269I GAATGC 2 cut(s) 1110, 1154
MvaI CCWGG 2 cut(s) 130, 1206
MwoI GCNNNNNNNGC 5 cut(s) 461, 664, 1107, 1397, 1495
NdeI CATATG 1 cut(s) 671
NdeII GATC 9 cut(s) 178, 328, 426, 544, 604, 842, 1240, 1416, 1585
NlaIV GGNNCC 3 cut(s) 440, 1418, 1604
NsiI ATGCAT 2 cut(s) 1146, 1294
NspI RCATGY 2 cut(s) 593, 741
PaeI GCATGC 1 cut(s) 741
PaeR7I CTCGAG 1 cut(s) 1652
PagI TCATGA 2 cut(s) 304, 601
PciI ACATGT 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 163
PctI GAATGC 2 cut(s) 1110, 1154
PfeI GAWTC 6 cut(s) 323, 875, 1162, 1513, 1541, 1664
PflFI GACNNNGTC 1 cut(s) 1703
PkrI GCNGC 6 cut(s) 523, 769, 898, 1154, 1262, 1628
PleI GAGTC 4 cut(s) 18, 1133, 1262, 1644
PpsI GAGTC 4 cut(s) 18, 1133, 1262, 1644
PscI ACATGT 1 cut(s) 589
Psp124BI GAGCTC 1 cut(s) 143
Psp6I CCWGG 2 cut(s) 128, 1204
PspGI CCWGG 2 cut(s) 128, 1204
PspN4I GGNNCC 3 cut(s) 440, 1418, 1604
PspPI GGNCC 2 cut(s) 479, 776
PspXI VCTCGAGB 1 cut(s) 1652
PsuI RGATCY 3 cut(s) 544, 842, 1416
PsyI GACNNNGTC 1 cut(s) 1703
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
RseI CAYNNNNRTG 3 cut(s) 18, 600, 1644
SacI GAGCTC 1 cut(s) 143
SapI GCTCTTC 1 cut(s) 163
SatI GCNGC 6 cut(s) 522, 768, 897, 1153, 1261, 1627
Sau3AI GATC 9 cut(s) 178, 328, 426, 544, 604, 842, 1240, 1416, 1585
Sau96I GGNCC 2 cut(s) 479, 776
SchI GAGTC 4 cut(s) 19, 1134, 1263, 1644
ScrFI CCNGG 2 cut(s) 130, 1206
SduI GDGCHC 5 cut(s) 126, 143, 597, 669, 1607
SfaNI GCATC 3 cut(s) 404, 541, 1071
SfcI CTRYAG 1 cut(s) 1440
Sfr274I CTCGAG 1 cut(s) 1652
SinI GGWCC 1 cut(s) 479
SlaI CTCGAG 1 cut(s) 1652
SmiMI CAYNNNNRTG 3 cut(s) 18, 600, 1644
SmlI CTYRAG 2 cut(s) 1199, 1652
SmoI CTYRAG 2 cut(s) 1199, 1652
SphI GCATGC 1 cut(s) 741
Sse9I AATT 7 cut(s) 225, 723, 753, 930, 1281, 1508, 1692
SsiI CCGC 3 cut(s) 1132, 1153, 1261
SspMI CTAG 8 cut(s) 18, 483, 579, 584, 747, 900, 1059, 1412
SstI GAGCTC 1 cut(s) 143
StyD4I CCNGG 2 cut(s) 128, 1204
TaaI ACNGT 5 cut(s) 631, 1040, 1309, 1560, 1688
TaiI ACGT 3 cut(s) 151, 918, 1362
TaqI TCGA 4 cut(s) 63, 1642, 1653, 1690
TaqII GACCGA 1 cut(s) 1315
TasI AATT 7 cut(s) 225, 723, 753, 930, 1281, 1508, 1692
TauI GCSGC 2 cut(s) 1155, 1263
TfiI GAWTC 6 cut(s) 323, 875, 1162, 1513, 1541, 1664
TscAI CASTG 3 cut(s) 1270, 1312, 1481
TseI GCWGC 4 cut(s) 521, 767, 896, 1626
TspDTI ATGAA 5 cut(s) 321, 1161, 1394, 1439, 1560
TspGWI ACGGA 2 cut(s) 634, 1409
TspRI CASTG 3 cut(s) 1270, 1312, 1481
Tth111I GACNNNGTC 1 cut(s) 1703
VneI GTGCAC 1 cut(s) 593
VpaK11BI GGWCC 1 cut(s) 479
XapI RAATTY 3 cut(s) 723, 753, 1692
XceI RCATGY 2 cut(s) 593, 741
XhoI CTCGAG 1 cut(s) 1652
XspI CTAG 8 cut(s) 18, 483, 579, 584, 747, 900, 1059, 1412
Zsp2I ATGCAT 2 cut(s) 1146, 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.