Rh2DG407700

C-5 cytosine-specific DNA methylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000338
Physical Location & Seq
Forward (+)
59077395 .. 59081561
4167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG407700.1

Sequence Viewer

Length: 1545 bp
ATGGGATTTTCTAAGAAGATGGTTGCCAATGCAATTCAGAAACATGGAGAAGAAAATACAGATGCAATTTTGGAAATGCTCCTCACATATTCGGCTCTAGAAAGTTCAACTGATGAACAGAAACAAATGGATTCTGATCACTTCTCCTCTGATTATGTGGAAAGCTTTCTAGATGGTTTTTCAGATATGGATAGCTGTGATGATGAGGAAATCATAAGTCATACATCTGAGGAGGAAAAACTAATGTCCTTGATCACAATGGGGTACACAGAAGAGGAGGCTTCCACGGCAGTAGAAAGATGTGGTCATAACTCCTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCAATTGGCAAAGGAAGAAGATGCCCTTCTTCCTCTTGAAGAGAAGCCCAGACTAAAACATGTGCACACTGATCCTAAGAAGAAGAGGAAAATTATTGTATCTGATATATCGAAAAGGAAAAGGCAGATGGGGCACATAGACACAGATACTGAGACACTTTGCCTCCCAAATCCAATGATTGGGTTTGGGATTCCAACTGGAGCATGGAAATCTAACAGAATTCTTCCAAAGGCAGCCATAGGGCCTCCATATTTTTATTATGAGAATGTCGCCCTTACTCCTAAAGGGGTTTGGGACACTATATCCAAATTCCTATATGATGTTCCCCCTGAATTTGTTGATTCAAAACATTTCTGTTCTGCTGCTAGAAAAAGGGGTTACGTCCATAATCTTCCTACTGAGAACAGATTTCCTCTTGTTCCGCTCCCCCCACAAACCATACATGAGGCATTTCCCTTGACAAAAAAATGGTGGCCTTCTTGGGACATAAGAACAAAGCTAAACTGTATAGTAACTTGCTATTCTAGTGCAAAGTTATCAGAGAGGATAATGAAGCTTCTCGAGGGCTGTGATGGCATTCCACCAGAAAGAGTCCAGCGGGCTGTTATACATGAATGTCGTAAGTGGAATCTATTGTGGGTTGGAAGAAATAGGGTTGCCCCACTTGAGCCTGATGAAGTGGAAATGCTCATGGGGTTTCCAAAGGATCACACAAGGGGAATGAGCCGCACTGATAGATACAAATCGCTTGGCAATGCATTTCAGATTCACACTGTGGCATACCACTTCTCAGTCTTGCGAGACAAGTTTCCAAATGGTATAAATGTCCTCTCTCTATTCTCCGGGATAGGTGGAGCAGAAGTAGCCCTCCATCGTCTTGGAATCCATATGAAGAATGTGGTGTCCGTAGAGATATCAGAAGTGAACAGAAACGTTGTGAGGTGTTGGTGGGAGCAAACAAACCAGACGGGAAATTTGATTCACCTTGCTGATGTAAAGGAGCTAGATGCTAACAGATTGGAACAGTTGATGTTTTCATTCGGTGGTTTTGATCTTGTAGTGGGTGGGAGCCCCTGCAATAATCTAACAGGCAGCAATAGACATCATCGAGATGGACTCGAGGGCAAAGAATCTTCACTTTTCTATCACTACTTTCGAATTCTGGACTTGGTCAAGTCTATTATGGCAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

514

Amino Acids

58.3

Weight (kDa)

6.07

Isoelectric Point (pI)

41.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 392 - 506 2.9e-09 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 530
AccBSI CCGCTC 1 cut(s) 775
AciI CCGC 3 cut(s) 773, 949, 1078
AclI AACGTT 1 cut(s) 1284
AclWI GGATC 2 cut(s) 416, 1065
AcsI RAATTY 5 cut(s) 570, 659, 683, 1324, 1509
AdeI CACNNNGTG 1 cut(s) 1126
AfaI GTAC 1 cut(s) 266
AfiI CCNNNNNNNGG 1 cut(s) 530
AflIII ACRYGT 1 cut(s) 409
AgsI TTSAA 3 cut(s) 108, 389, 696
AluBI AGCT 5 cut(s) 165, 195, 850, 907, 1354
AluI AGCT 5 cut(s) 165, 195, 850, 907, 1354
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 497, 1146
Alw44I GTGCAC 1 cut(s) 413
AlwI GGATC 2 cut(s) 416, 1065
AlwNI CAGNNNCTG 1 cut(s) 500
Ama87I CYCGRG 2 cut(s) 911, 1469
AoxI GGCC 2 cut(s) 593, 824
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 4 cut(s) 347, 584, 713, 1443
ApoI RAATTY 5 cut(s) 570, 659, 683, 1324, 1509
ArsI GACNNNNNNTTYG 2 cut(s) 1309, 1341
Asp700I GAANNNNTTC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 593
AsuC2I CCSGG 1 cut(s) 1195
AsuHPI GGTGA 1 cut(s) 1325
AsuII TTCGAA 1 cut(s) 1507
AvaI CYCGRG 2 cut(s) 911, 1469
BaeGI GKGCMC 2 cut(s) 417, 486
BanII GRGCYC 1 cut(s) 1424
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 4 cut(s) 334, 596, 700, 1455
BccI CCATC 6 cut(s) 13, 167, 472, 917, 1230, 1457
BceAI ACGGC 1 cut(s) 303
BclI TGATCA 2 cut(s) 136, 252
BcnI CCSGG 1 cut(s) 1195
BcoDI GTCTC 2 cut(s) 497, 1146
BfaI CTAG 5 cut(s) 98, 170, 717, 876, 1355
BisI GCNGC 5 cut(s) 348, 585, 714, 1078, 1444
BlsI GCNGC 5 cut(s) 349, 586, 715, 1079, 1445
Bme1390I CCNGG 1 cut(s) 1195
BmeT110I CYCGRG 2 cut(s) 911, 1469
BmgT120I GGNCC 1 cut(s) 593
BmiI GGNNCC 1 cut(s) 1421
BmrFI CCNGG 1 cut(s) 1195
BmsI GCATC 3 cut(s) 52, 361, 1348
BplI GAGNNNNNCTC 2 cut(s) 1452, 1484
BpmI CTGGAG 1 cut(s) 570
Bpu14I TTCGAA 1 cut(s) 1507
BpuEI CTTGAG 1 cut(s) 1037
BpuMI CCSGG 1 cut(s) 1195
BsaBI GATNNNNATC 2 cut(s) 135, 1093
BsaJI CCNNGG 1 cut(s) 285
BsaXI ACNNNNNCTCC 4 cut(s) 498, 528, 1197, 1227
Bsc4I CCNNNNNNNGG 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 553
Bse3DI GCAATG 1 cut(s) 1111
Bse8I GATNNNNATC 2 cut(s) 135, 1093
BseDI CCNNGG 1 cut(s) 285
BseJI GATNNNNATC 2 cut(s) 135, 1093
BseLI CCNNNNNNNGG 1 cut(s) 530
BseMI GCAATG 1 cut(s) 1111
BseMII CTCAG 4 cut(s) 219, 492, 741, 1155
BseNI ACTGG 1 cut(s) 553
BseRI GAGGAG 5 cut(s) 71, 136, 245, 290, 304
BseSI GKGCMC 2 cut(s) 417, 486
BseXI GCAGC 4 cut(s) 334, 596, 700, 1455
BshFI GGCC 2 cut(s) 595, 826
BsiHKAI GWGCWC 1 cut(s) 417
BsiHKCI CYCGRG 2 cut(s) 911, 1469
BsiSI CCGG 1 cut(s) 1194
BslFI GGGAC 2 cut(s) 659, 848
BslI CCNNNNNNNGG 1 cut(s) 530
BsmAI GTCTC 2 cut(s) 497, 1146
BsmFI GGGAC 2 cut(s) 659, 848
BsmI GAATGC 1 cut(s) 927
BsnI GGCC 2 cut(s) 595, 826
BsoBI CYCGRG 2 cut(s) 911, 1469
Bsp119I TTCGAA 1 cut(s) 1507
Bsp1286I GDGCHC 3 cut(s) 417, 486, 1424
Bsp143I GATC 5 cut(s) 136, 252, 421, 1057, 1402
BspACI CCGC 3 cut(s) 773, 949, 1078
BspANI GGCC 2 cut(s) 595, 826
BspCNI CTCAG 4 cut(s) 220, 493, 742, 1154
BspLI GGNNCC 1 cut(s) 1421
BspPI GGATC 2 cut(s) 416, 1065
BspT104I TTCGAA 1 cut(s) 1507
BsrBI CCGCTC 1 cut(s) 775
BsrDI GCAATG 1 cut(s) 1111
BsrI ACTGG 1 cut(s) 553
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 5 cut(s) 136, 252, 421, 1057, 1402
Bst4CI ACNGT 3 cut(s) 857, 1126, 1377
Bst6I CTCTTC 3 cut(s) 267, 384, 428
BstBI TTCGAA 1 cut(s) 1507
BstC8I GCNNGC 1 cut(s) 951
BstDEI CTNAG 6 cut(s) 12, 228, 426, 501, 750, 1141
BstDSI CCRYGG 1 cut(s) 285
BstKTI GATC 5 cut(s) 139, 255, 424, 1060, 1405
BstMAI GTCTC 2 cut(s) 497, 1146
BstMBI GATC 5 cut(s) 136, 252, 421, 1057, 1402
BstMWI GCNNNNNNNGC 5 cut(s) 287, 356, 481, 924, 1214
BstNSI RCATGY 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 1193
BstSLI GKGCMC 2 cut(s) 417, 486
BstV1I GCAGC 4 cut(s) 334, 596, 700, 1455
BstXI CCANNNNNNTGG 1 cut(s) 1229
BsuRI GGCC 2 cut(s) 595, 826
BtgI CCRYGG 1 cut(s) 285
BtsIMutI CAGTG 3 cut(s) 417, 1080, 1122
Cac8I GCNNGC 1 cut(s) 951
CaiI CAGNNNCTG 1 cut(s) 500
Cfr13I GGNCC 1 cut(s) 593
Csp6I GTAC 1 cut(s) 265
CviAII CATG 6 cut(s) 44, 410, 555, 794, 962, 1042
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 6 cut(s) 12, 228, 426, 501, 750, 1141
DpnI GATC 5 cut(s) 138, 254, 423, 1059, 1404
DpnII GATC 5 cut(s) 136, 252, 421, 1057, 1402
DraIII CACNNNGTG 1 cut(s) 1126
Eam1104I CTCTTC 3 cut(s) 267, 384, 428
EarI CTCTTC 3 cut(s) 267, 384, 428
Eco24I GRGCYC 1 cut(s) 1424
Eco32I GATATC 1 cut(s) 1266
Eco88I CYCGRG 2 cut(s) 911, 1469
EcoO109I RGGNCCY 1 cut(s) 593
EcoRI GAATTC 2 cut(s) 570, 1509
EcoRV GATATC 1 cut(s) 1266
EcoT22I ATGCAT 1 cut(s) 1111
EcoT38I GRGCYC 1 cut(s) 1424
FaeI CATG 6 cut(s) 47, 413, 558, 797, 965, 1045
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FaqI GGGAC 2 cut(s) 659, 848
FatI CATG 6 cut(s) 43, 409, 554, 793, 961, 1041
FauI CCCGC 1 cut(s) 942
FauNDI CATATG 1 cut(s) 1239
FbaI TGATCA 2 cut(s) 136, 252
Fnu4HI GCNGC 5 cut(s) 348, 585, 714, 1078, 1444
FriOI GRGCYC 1 cut(s) 1424
Fsp4HI GCNGC 5 cut(s) 348, 585, 714, 1078, 1444
FspBI CTAG 5 cut(s) 98, 170, 717, 876, 1355
GluI GCNGC 5 cut(s) 348, 585, 714, 1078, 1444
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 2 cut(s) 595, 826
HapII CCGG 1 cut(s) 1194
Hin1II CATG 6 cut(s) 47, 413, 558, 797, 965, 1045
HindIII AAGCTT 2 cut(s) 163, 905
HpaII CCGG 1 cut(s) 1194
HphI GGTGA 1 cut(s) 1325
Hpy166II GTNNAC 3 cut(s) 267, 415, 1276
Hpy188I TCNGA 9 cut(s) 39, 136, 151, 184, 229, 454, 892, 1116, 1270
Hpy188III TCNNGA 7 cut(s) 98, 170, 323, 386, 911, 1460, 1514
Hpy8I GTNNAC 3 cut(s) 267, 415, 1276
HpyAV CCTTC 2 cut(s) 386, 837
HpyCH4III ACNGT 3 cut(s) 857, 1126, 1377
HpyCH4IV ACGT 2 cut(s) 732, 1284
HpyCH4V TGCA 6 cut(s) 32, 65, 415, 881, 1109, 1428
HpyF10VI GCNNNNNNNGC 5 cut(s) 287, 356, 481, 924, 1214
HpyF3I CTNAG 6 cut(s) 12, 228, 426, 501, 750, 1141
HpySE526I ACGT 2 cut(s) 732, 1284
Hsp92II CATG 6 cut(s) 47, 413, 558, 797, 965, 1045
Ksp22I TGATCA 2 cut(s) 136, 252
Kzo9I GATC 5 cut(s) 136, 252, 421, 1057, 1402
LmnI GCTCC 7 cut(s) 84, 551, 780, 1205, 1303, 1351, 1419
Lsp1109I GCAGC 4 cut(s) 334, 596, 700, 1455
LweI GCATC 3 cut(s) 52, 361, 1348
MaeI CTAG 5 cut(s) 98, 170, 717, 876, 1355
MaeII ACGT 2 cut(s) 732, 1284
MaeIII GTNAC 2 cut(s) 728, 862
MalI GATC 5 cut(s) 138, 254, 423, 1059, 1404
MbiI CCGCTC 1 cut(s) 775
MboI GATC 5 cut(s) 136, 252, 421, 1057, 1402
MfeI CAATTG 1 cut(s) 353
MhlI GDGCHC 3 cut(s) 417, 486, 1424
MlyI GAGTC 2 cut(s) 951, 1461
MmeI TCCRAC 2 cut(s) 569, 973
Mph1103I ATGCAT 1 cut(s) 1111
MroXI GAANNNNTTC 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 1461
MspA1I CMGCKG 1 cut(s) 949
MspI CCGG 1 cut(s) 1194
MspR9I CCNGG 1 cut(s) 1195
MunI CAATTG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 927
MwoI GCNNNNNNNGC 5 cut(s) 287, 356, 481, 924, 1214
NciI CCSGG 1 cut(s) 1195
NdeI CATATG 1 cut(s) 1239
NdeII GATC 5 cut(s) 136, 252, 421, 1057, 1402
NlaIII CATG 6 cut(s) 47, 413, 558, 797, 965, 1045
NlaIV GGNNCC 1 cut(s) 1421
NsiI ATGCAT 1 cut(s) 1111
NspI RCATGY 1 cut(s) 413
NspV TTCGAA 1 cut(s) 1507
PaeR7I CTCGAG 2 cut(s) 911, 1469
PciI ACATGT 1 cut(s) 409
PctI GAATGC 1 cut(s) 927
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 8 cut(s) 131, 541, 692, 979, 1117, 1233, 1330, 1481
PflFI GACNNNGTC 1 cut(s) 1520
PflMI CCANNNNNTGG 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 1193
PkrI GCNGC 5 cut(s) 349, 586, 715, 1079, 1445
PleI GAGTC 2 cut(s) 950, 1461
PpsI GAGTC 2 cut(s) 950, 1461
PscI ACATGT 1 cut(s) 409
Psp1406I AACGTT 1 cut(s) 1284
PspN4I GGNNCC 1 cut(s) 1421
PspPI GGNCC 1 cut(s) 593
PspXI VCTCGAGB 1 cut(s) 1469
PstNI CAGNNNCTG 1 cut(s) 500
PsyI GACNNNGTC 1 cut(s) 1520
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
RseI CAYNNNNRTG 1 cut(s) 1461
SatI GCNGC 5 cut(s) 348, 585, 714, 1078, 1444
Sau3AI GATC 5 cut(s) 136, 252, 421, 1057, 1402
Sau96I GGNCC 1 cut(s) 593
SchI GAGTC 2 cut(s) 951, 1461
ScrFI CCNGG 1 cut(s) 1195
SduI GDGCHC 3 cut(s) 417, 486, 1424
SfaNI GCATC 3 cut(s) 52, 361, 1348
Sfr274I CTCGAG 2 cut(s) 911, 1469
SfuI TTCGAA 1 cut(s) 1507
SlaI CTCGAG 2 cut(s) 911, 1469
SmiMI CAYNNNNRTG 1 cut(s) 1461
SmlI CTYRAG 3 cut(s) 911, 1016, 1469
SmoI CTYRAG 3 cut(s) 911, 1016, 1469
SsiI CCGC 3 cut(s) 773, 949, 1078
SspMI CTAG 5 cut(s) 98, 170, 717, 876, 1355
StyD4I CCNGG 1 cut(s) 1193
TaaI ACNGT 3 cut(s) 857, 1126, 1377
TaiI ACGT 2 cut(s) 735, 1287
TaqI TCGA 5 cut(s) 461, 912, 1459, 1470, 1507
TauI GCSGC 1 cut(s) 1080
TfiI GAWTC 8 cut(s) 131, 541, 692, 979, 1117, 1233, 1330, 1481
TscAI CASTG 3 cut(s) 424, 1087, 1129
TseI GCWGC 4 cut(s) 347, 584, 713, 1443
TspDTI ATGAA 6 cut(s) 129, 917, 978, 1041, 1256, 1377
TspGWI ACGGA 1 cut(s) 1246
TspRI CASTG 3 cut(s) 424, 1087, 1129
Tth111I GACNNNGTC 1 cut(s) 1520
Van91I CCANNNNNTGG 1 cut(s) 530
VneI GTGCAC 1 cut(s) 413
XapI RAATTY 5 cut(s) 570, 659, 683, 1324, 1509
XbaI TCTAGA 2 cut(s) 97, 169
XceI RCATGY 1 cut(s) 413
XcmI CCANNNNNNNNNTGG 1 cut(s) 552
XhoI CTCGAG 2 cut(s) 911, 1469
XmnI GAANNNNTTC 1 cut(s) 165
XspI CTAG 5 cut(s) 98, 170, 717, 876, 1355
Zsp2I ATGCAT 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.