Rh2BG648200

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000338
Physical Location & Seq
Forward (+)
86741642 .. 86744169
2528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG648200.1

Sequence Viewer

Length: 573 bp
ATGCTATTACTTGGCGTGGAGGATGGAAATACATCTGGGGTAAACGGCAATAATGTTGACTGGGATACTGAAGATGAGCTTGACGAGATTGAAAATTTCACTTTATCTTCTTCTTCAGCTAATCTGGGCTCTGGGGAGATTGATACAGTAGCCTATCATCTCTCTGTGTTGAAAGATCGGTTCCCCAATGGCATCAATCTCCTCTCTCTTTTCTCTGGAATTGGCGGTGCAGAGATAGCCCTCCATCGGCTTGGCATTCGAATGAAGAATGTTGTGTCTGTTGAGATTTCAGTGGTGAGCAGAACTGTTGTGAGGACTTGGTGGGAGCAAACCAACCAAAAAGGAAACTTGTATCACCTCGCTGATGTGCAAGAGTTGAATGCCGACCGGTTGGAGCATTACATCAATACATTTGGTGGGTTTGATCTCGTGGTTGGTGGGAGCCCGTGCAACAATCTTGCCGGTAGCAACAGACATCATAGGGATGGGCTTGAGGGAAAAGAATCTTCTCTATTTTATGATTATTTCTGTATATTAGACTTAGTGAAGGGTCTTATGGCAAGATATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

190

Amino Acids

20.95

Weight (kDa)

4.77

Isoelectric Point (pI)

26.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 66 - 179 4.3e-09 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 225
AcsI RAATTY 1 cut(s) 94
AcuI CTGAAG 2 cut(s) 90, 99
AfiI CCNNNNNNNGG 1 cut(s) 246
AgeI ACCGGT 1 cut(s) 387
AgsI TTSAA 3 cut(s) 92, 172, 379
AluBI AGCT 3 cut(s) 79, 119, 570
AluI AGCT 3 cut(s) 79, 119, 570
ApoI RAATTY 1 cut(s) 94
AsiGI ACCGGT 1 cut(s) 387
AsuHPI GGTGA 2 cut(s) 307, 347
AsuII TTCGAA 1 cut(s) 259
BanII GRGCYC 2 cut(s) 131, 446
BauI CACGAG 1 cut(s) 428
BccI CCATC 3 cut(s) 17, 252, 479
BceAI ACGGC 1 cut(s) 61
BciVI GTATCC 1 cut(s) 58
BfuI GTATCC 1 cut(s) 58
BmiI GGNNCC 2 cut(s) 182, 443
BmrI ACTGGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 70
Bpu14I TTCGAA 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 512
BsaWI WCCGGW 1 cut(s) 387
Bsc4I CCNNNNNNNGG 1 cut(s) 246
Bse118I RCCGGY 2 cut(s) 387, 461
Bse1I ACTGG 1 cut(s) 65
BseGI GGATG 2 cut(s) 28, 490
BseLI CCNNNNNNNGG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 65
BseRI GAGGAG 1 cut(s) 191
BsgI GTGCAG 1 cut(s) 249
Bsh1285I CGRYCG 1 cut(s) 388
BshTI ACCGGT 1 cut(s) 387
BsiEI CGRYCG 1 cut(s) 388
BsiSI CCGG 2 cut(s) 388, 462
BslI CCNNNNNNNGG 1 cut(s) 246
BsmI GAATGC 2 cut(s) 255, 385
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 2 cut(s) 131, 446
Bsp143I GATC 2 cut(s) 175, 424
BspACI CCGC 1 cut(s) 225
BspLI GGNNCC 2 cut(s) 182, 443
BspT104I TTCGAA 1 cut(s) 259
BsrFI RCCGGY 2 cut(s) 387, 461
BsrI ACTGG 1 cut(s) 65
BssAI RCCGGY 2 cut(s) 387, 461
BssMI GATC 2 cut(s) 175, 424
BssSI CACGAG 1 cut(s) 428
Bst2BI CACGAG 1 cut(s) 428
Bst4CI ACNGT 2 cut(s) 148, 307
BstBI TTCGAA 1 cut(s) 259
BstDEI CTNAG 1 cut(s) 541
BstF5I GGATG 2 cut(s) 28, 490
BstKTI GATC 2 cut(s) 178, 427
BstMBI GATC 2 cut(s) 175, 424
BstMCI CGRYCG 1 cut(s) 388
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstXI CCANNNNNNTGG 1 cut(s) 251
BsuI GTATCC 1 cut(s) 58
BtsCI GGATG 2 cut(s) 28, 490
BtsIMutI CAGTG 1 cut(s) 297
Cfr10I RCCGGY 2 cut(s) 387, 461
CspAI ACCGGT 1 cut(s) 387
CviJI RGCY 9 cut(s) 79, 119, 129, 152, 239, 250, 444, 490, 570
CviKI_1 RGCY 9 cut(s) 79, 119, 129, 152, 239, 250, 444, 490, 570
DdeI CTNAG 1 cut(s) 541
DpnI GATC 2 cut(s) 177, 426
DpnII GATC 2 cut(s) 175, 424
Eco24I GRGCYC 2 cut(s) 131, 446
Eco57I CTGAAG 2 cut(s) 90, 99
EcoT38I GRGCYC 2 cut(s) 131, 446
FaiI YATR 5 cut(s) 480, 519, 533, 557, 567
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FokI GGATG 2 cut(s) 35, 497
FriOI GRGCYC 2 cut(s) 131, 446
HapII CCGG 2 cut(s) 388, 462
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 1 cut(s) 503
HpaII CCGG 2 cut(s) 388, 462
HphI GGTGA 2 cut(s) 307, 347
Hpy166II GTNNAC 2 cut(s) 43, 58
Hpy188III TCNNGA 1 cut(s) 216
Hpy8I GTNNAC 2 cut(s) 43, 58
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 2 cut(s) 148, 307
HpyCH4V TGCA 3 cut(s) 230, 370, 450
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 1 cut(s) 541
Kzo9I GATC 2 cut(s) 175, 424
LmnI GCTCC 3 cut(s) 325, 394, 441
LpnPI CCDG 7 cut(s) 21, 46, 110, 117, 201, 401, 475
LweI GCATC 1 cut(s) 201
MalI GATC 2 cut(s) 177, 426
MboI GATC 2 cut(s) 175, 424
MboII GAAGA 6 cut(s) 83, 99, 102, 105, 277, 498
MhlI GDGCHC 2 cut(s) 131, 446
MluCI AATT 2 cut(s) 94, 219
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 6 cut(s) 13, 212, 251, 306, 368, 487
MslI CAYNNNNRTG 2 cut(s) 260, 483
MspI CCGG 2 cut(s) 388, 462
Mva1269I GAATGC 2 cut(s) 255, 385
MwoI GCNNNNNNNGC 1 cut(s) 236
NdeII GATC 2 cut(s) 175, 424
NlaIV GGNNCC 2 cut(s) 182, 443
NspV TTCGAA 1 cut(s) 259
PctI GAATGC 2 cut(s) 255, 385
PfeI GAWTC 1 cut(s) 503
PinAI ACCGGT 1 cut(s) 387
PspN4I GGNNCC 2 cut(s) 182, 443
RseI CAYNNNNRTG 2 cut(s) 260, 483
Sau3AI GATC 2 cut(s) 175, 424
SduI GDGCHC 2 cut(s) 131, 446
SetI ASST 4 cut(s) 81, 121, 360, 572
SfaNI GCATC 1 cut(s) 201
SfuI TTCGAA 1 cut(s) 259
SmiMI CAYNNNNRTG 2 cut(s) 260, 483
SmlI CTYRAG 1 cut(s) 491
SmoI CTYRAG 1 cut(s) 491
Sse9I AATT 2 cut(s) 94, 219
SsiI CCGC 1 cut(s) 225
TaaI ACNGT 2 cut(s) 148, 307
TaqI TCGA 1 cut(s) 259
TasI AATT 2 cut(s) 94, 219
TfiI GAWTC 1 cut(s) 503
TscAI CASTG 1 cut(s) 297
TspDTI ATGAA 1 cut(s) 278
TspRI CASTG 1 cut(s) 297
XapI RAATTY 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.