Rmu_sc0001299.1_g000016

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001299.1
OG0000338
Physical Location & Seq
Reverse (-)
78735 .. 84685
5951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001299.1_g000016.1.cds

Sequence Viewer

Length: 2085 bp
atgtcgttaagtttgttacgcgcaccaggtggcagtcgttccttccatttccgtcagcagtatttgagctcggattccgttacagacaagtctgccatcgctttctcttctgcatgcgcctctaacctcttctccttctttttcttcttcaccgtcacatcacagaggtcctctgcattttcacctcaggatggaagtacttctggtgtagaagacaatgatattgattggaacacagaagatgagcttgaattggacaacttcactttatctccttgttctagtttgctacttactggtggcgaagctaatgccttctctggggaggcgagctcatgtccaagttcttccaactccaaagttcttgatcagtttatagggatgggattttctaagaagatggttgaaaaagcaattcaggaacatggagaagaaaatacagatgcaattctggaaatgctcctctcagattcagtgagttttaacataacttcagataccaatgcggttctagaaagttcttcccaagaactgcaacaaaccgattcagatcacttttcctctgattatgatggaagctttttggatgacttttcagatttggaaagctctggtgatgaggaaatcatgagtcctacatccgaggaggaaaagctactcatgtccttgatcaaaatggggtacacagaagaggaggcttccatagctatagaaagatgtggtcttagctcttcacttctggaattgactgattttttatctgctgctcatttggcaaaggcagaagatgcccttcttcctctagaagagaagcctggactaaaacatttgcacactgattatcctaagaagaaaaggaaacttttggttgataaagtgtcgaaaaggaaaaggcaaaataggctcgtaggcgaggataatgagacagttcgtctcccaaatccaatgattgggtttggtattccaactggaccatgccaatcttacagatatcttccaacggcagccataggacctccctatttttattatgagaatgtagccctcactcctaaaggggtttgggacactatatcgagattcctatatgatgtgccacctgaatttgttgactctaagtacttctgtgctgctgctagaaaaagaggttatgttcacaatcttccaatcgagaatagaatccctctcgttccacttcccccacaaaccatacatgaggcattgcccatgtcaagaaagtggtggccttcttgggacacacggacaaagctaaattgcttacaaacttgccattcaagtgcaaagttaacagagaggatcatgaaggctcttgagggctatgatgacattccaccagagagagtccagcggggtgtaatgcatgaatgccgtaagtggaatctagtgtgggttggaaaaaatagggtcgccccacttgagcctgatgaatttgaaatgcttttggggtttccaaaggaccacaccaggggaataagcaggactgatagatataaatcactcgggaatgcatttcagattgacaccgtcgcataccacttctctgttttgcgggacatgtttcccaatggcatcaatgtcctctcccttttctctgggataggtggggcagaaatagcccttcatcggcttggtatccgtatgaagaatgttgtgtctctagagatttcagaagttaacagaaacgttctgaggtgttggtgggagcaaacaaaccagagggggaatttgattcaccttcctgatgtgcaagagcttaatgctgaccgcttggaacagtttatgagttcatttggtggctttgatctagtagttggtggcagtccctgcaacaatcttacaggtagcaatcgatatcatagagatggactcgaggctatgttgtcactgaaaccctggacctgcttcgcttcgtacttcaagaactcgtcctccctgtgcttgattgagaacaagtgcattgcagttttcttgggcactgttagtattgtttgtgctgcaagttttaagcttcaggacttgttggtaggaatggtaggcatcttcaagtttgcagacttgcagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

694

Amino Acids

77.58

Weight (kDa)

5.24

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1928
AccB7I CCANNNNNTGG 1 cut(s) 950
AccII CGCG 1 cut(s) 21
AciI CCGC 4 cut(s) 506, 1369, 1570, 1786
AclI AACGTT 1 cut(s) 1704
AclWI GGATC 1 cut(s) 1325
AcsI RAATTY 3 cut(s) 1103, 1448, 1744
AcuI CTGAAG 2 cut(s) 477, 2015
AdeI CACNNNGTG 1 cut(s) 29
AfaI GTAC 4 cut(s) 199, 683, 1121, 1934
AfiI CCNNNNNNNGG 5 cut(s) 191, 321, 950, 1486, 1644
AflIII ACRYGT 1 cut(s) 1575
AgsI TTSAA 6 cut(s) 251, 407, 1296, 1454, 1939, 2065
AhdI GACNNNNNGTC 1 cut(s) 930
AjnI CCWGG 4 cut(s) 25, 814, 1484, 1913
AloI GAACNNNNNNTCC 2 cut(s) 1934, 1966
Alw21I GWGCWC 2 cut(s) 71, 335
Alw26I GTCTC 3 cut(s) 917, 938, 1680
AlwI GGATC 1 cut(s) 1325
AlwNI CAGNNNCTG 1 cut(s) 1845
Ama87I CYCGRG 2 cut(s) 1520, 1889
AoxI GGCC 1 cut(s) 1244
ApeKI GCWGC 5 cut(s) 764, 1004, 1130, 1133, 2015
ApoI RAATTY 3 cut(s) 1103, 1448, 1744
Asp700I GAANNNNTTC 1 cut(s) 199
AspLEI GCGC 2 cut(s) 23, 119
AspS9I GGNCC 5 cut(s) 168, 971, 1013, 1477, 1917
AsuHPI GGTGA 4 cut(s) 142, 174, 626, 1745
AvaI CYCGRG 2 cut(s) 1520, 1889
AvaII GGWCC 5 cut(s) 168, 971, 1013, 1477, 1917
AxyI CCTNAGG 1 cut(s) 186
BaeGI GKGCMC 1 cut(s) 1997
BanII GRGCYC 2 cut(s) 71, 335
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 2 cut(s) 71, 335
BbvI GCAGC 5 cut(s) 751, 1016, 1117, 1120, 2002
BccI CCATC 6 cut(s) 104, 185, 376, 394, 566, 1877
BceAI ACGGC 2 cut(s) 1017, 1374
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 4 cut(s) 27, 816, 1486, 1915
BciVI GTATCC 1 cut(s) 1664
BclI TGATCA 2 cut(s) 367, 669
BcoDI GTCTC 3 cut(s) 917, 938, 1680
BfaI CTAG 7 cut(s) 282, 512, 803, 1137, 1403, 1679, 1826
BfmI CTRYAG 1 cut(s) 708
BfuAI ACCTGC 1 cut(s) 1928
BfuI GTATCC 1 cut(s) 1664
BisI GCNGC 5 cut(s) 765, 1005, 1131, 1134, 2016
BlsI GCNGC 5 cut(s) 766, 1006, 1132, 1135, 2017
BmcAI AGTACT 2 cut(s) 199, 1121
Bme1390I CCNGG 4 cut(s) 27, 816, 1486, 1915
Bme18I GGWCC 5 cut(s) 168, 971, 1013, 1477, 1917
BmeRI GACNNNNNGTC 1 cut(s) 930
BmeT110I CYCGRG 2 cut(s) 1520, 1889
BmgT120I GGNCC 5 cut(s) 168, 971, 1013, 1477, 1917
BmrFI CCNGG 4 cut(s) 27, 816, 1486, 1915
BmsI GCATC 4 cut(s) 433, 778, 1599, 2067
BpiI GAAGAC 1 cut(s) 219
BplI GAGNNNNNCTC 4 cut(s) 317, 349, 1872, 1904
BpuEI CTTGAG 2 cut(s) 1352, 1457
Bsa29I ATCGAT 1 cut(s) 1870
BsaBI GATNNNNATC 2 cut(s) 549, 1513
BsaJI CCNNGG 3 cut(s) 642, 1485, 1913
BsaXI ACNNNNNCTCC 6 cut(s) 116, 146, 256, 286, 1934, 1964
Bsc4I CCNNNNNNNGG 5 cut(s) 191, 321, 950, 1486, 1644
Bse1I ACTGG 2 cut(s) 301, 973
Bse21I CCTNAGG 1 cut(s) 186
Bse3DI GCAATG 2 cut(s) 1220, 1977
Bse8I GATNNNNATC 2 cut(s) 549, 1513
BseBI CCWGG 4 cut(s) 27, 816, 1486, 1915
BseCI ATCGAT 1 cut(s) 1870
BseDI CCNNGG 3 cut(s) 642, 1485, 1913
BseGI GGATG 4 cut(s) 196, 387, 592, 638
BseJI GATNNNNATC 2 cut(s) 549, 1513
BseLI CCNNNNNNNGG 5 cut(s) 191, 321, 950, 1486, 1644
BseMI GCAATG 2 cut(s) 1220, 1977
BseMII CTCAG 3 cut(s) 200, 480, 1700
BseNI ACTGG 2 cut(s) 301, 973
BseRI GAGGAG 3 cut(s) 452, 659, 707
BseSI GKGCMC 1 cut(s) 1997
BseXI GCAGC 5 cut(s) 751, 1016, 1117, 1120, 2002
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 1246
BshVI ATCGAT 1 cut(s) 1870
BsiHKAI GWGCWC 2 cut(s) 71, 335
BsiHKCI CYCGRG 2 cut(s) 1520, 1889
BslFI GGGAC 4 cut(s) 1079, 1268, 1586, 1827
BslI CCNNNNNNNGG 5 cut(s) 191, 321, 950, 1486, 1644
BsmAI GTCTC 3 cut(s) 917, 938, 1680
BsmBI CGTCTC 1 cut(s) 938
BsmFI GGGAC 4 cut(s) 1079, 1268, 1586, 1827
BsmI GAATGC 2 cut(s) 1391, 1531
BsnI GGCC 1 cut(s) 1246
BsoBI CYCGRG 2 cut(s) 1520, 1889
Bsp1286I GDGCHC 3 cut(s) 71, 335, 1997
Bsp143I GATC 5 cut(s) 367, 550, 669, 1317, 1822
BspACI CCGC 4 cut(s) 506, 1369, 1570, 1786
BspANI GGCC 1 cut(s) 1246
BspCNI CTCAG 3 cut(s) 199, 479, 1701
BspDI ATCGAT 1 cut(s) 1870
BspFNI CGCG 1 cut(s) 21
BspHI TCATGA 2 cut(s) 627, 1320
BspMI ACCTGC 1 cut(s) 1928
BspPI GGATC 1 cut(s) 1325
BspQI GCTCTTC 1 cut(s) 736
BsrDI GCAATG 2 cut(s) 1220, 1977
BsrI ACTGG 2 cut(s) 301, 973
BssECI CCNNGG 3 cut(s) 642, 1485, 1913
BssMI GATC 5 cut(s) 367, 550, 669, 1317, 1822
Bst2UI CCWGG 4 cut(s) 27, 816, 1486, 1915
Bst4CI ACNGT 5 cut(s) 154, 928, 1546, 1797, 1999
Bst6I CTCTTC 5 cut(s) 112, 134, 684, 736, 801
BstAPI GCANNNNNTGC 2 cut(s) 788, 1845
BstC8I GCNNGC 2 cut(s) 115, 331
BstDEI CTNAG 7 cut(s) 186, 393, 466, 725, 846, 1116, 1709
BstF5I GGATG 4 cut(s) 196, 387, 592, 638
BstFNI CGCG 1 cut(s) 21
BstHHI GCGC 2 cut(s) 23, 119
BstKTI GATC 5 cut(s) 370, 553, 672, 1320, 1825
BstMAI GTCTC 3 cut(s) 917, 938, 1680
BstMBI GATC 5 cut(s) 367, 550, 669, 1317, 1822
BstMWI GCNNNNNNNGC 6 cut(s) 704, 773, 788, 901, 1634, 1845
BstNI CCWGG 4 cut(s) 27, 816, 1486, 1915
BstNSI RCATGY 2 cut(s) 117, 1579
BstSCI CCNGG 4 cut(s) 25, 814, 1484, 1913
BstSFI CTRYAG 1 cut(s) 708
BstSLI GKGCMC 1 cut(s) 1997
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 5 cut(s) 751, 1016, 1117, 1120, 2002
BstV2I GAAGAC 1 cut(s) 219
Bsu15I ATCGAT 1 cut(s) 1870
Bsu36I CCTNAGG 1 cut(s) 186
BsuI GTATCC 1 cut(s) 1664
BsuRI GGCC 1 cut(s) 1246
BsuTUI ATCGAT 1 cut(s) 1870
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 4 cut(s) 196, 387, 592, 638
BtsIMutI CAGTG 4 cut(s) 480, 834, 1904, 1995
BveI ACCTGC 1 cut(s) 1928
Cac8I GCNNGC 2 cut(s) 115, 331
CaiI CAGNNNCTG 1 cut(s) 1845
CciI TCATGA 2 cut(s) 627, 1320
CfoI GCGC 2 cut(s) 23, 119
Cfr13I GGNCC 5 cut(s) 168, 971, 1013, 1477, 1917
ClaI ATCGAT 1 cut(s) 1870
CsiI ACCWGGT 1 cut(s) 25
Csp6I GTAC 4 cut(s) 198, 682, 1120, 1933
CviQI GTAC 4 cut(s) 198, 682, 1120, 1933
DdeI CTNAG 7 cut(s) 186, 393, 466, 725, 846, 1116, 1709
DpnI GATC 5 cut(s) 369, 552, 671, 1319, 1824
DpnII GATC 5 cut(s) 367, 550, 669, 1317, 1822
DraIII CACNNNGTG 1 cut(s) 29
DriI GACNNNNNGTC 1 cut(s) 930
Eam1104I CTCTTC 5 cut(s) 112, 134, 684, 736, 801
Eam1105I GACNNNNNGTC 1 cut(s) 930
EarI CTCTTC 5 cut(s) 112, 134, 684, 736, 801
Ecl136II GAGCTC 2 cut(s) 69, 333
Eco24I GRGCYC 2 cut(s) 71, 335
Eco32I GATATC 2 cut(s) 992, 1874
Eco47I GGWCC 5 cut(s) 168, 971, 1013, 1477, 1917
Eco53kI GAGCTC 2 cut(s) 69, 333
Eco57I CTGAAG 2 cut(s) 477, 2015
Eco81I CCTNAGG 1 cut(s) 186
Eco88I CYCGRG 2 cut(s) 1520, 1889
EcoICRI GAGCTC 2 cut(s) 69, 333
EcoO109I RGGNCCY 2 cut(s) 168, 1013
EcoRII CCWGG 4 cut(s) 25, 814, 1484, 1913
EcoRV GATATC 2 cut(s) 992, 1874
EcoT22I ATGCAT 2 cut(s) 1383, 1531
EcoT38I GRGCYC 2 cut(s) 71, 335
Esp3I CGTCTC 1 cut(s) 938
FalI AAGNNNNNCTT 4 cut(s) 231, 263, 777, 809
FaqI GGGAC 4 cut(s) 1079, 1268, 1586, 1827
FauI CCCGC 2 cut(s) 1362, 1563
FbaI TGATCA 2 cut(s) 367, 669
Fnu4HI GCNGC 5 cut(s) 765, 1005, 1131, 1134, 2016
FokI GGATG 4 cut(s) 203, 394, 599, 625
FriOI GRGCYC 2 cut(s) 71, 335
Fsp4HI GCNGC 5 cut(s) 765, 1005, 1131, 1134, 2016
FspBI CTAG 7 cut(s) 282, 512, 803, 1137, 1403, 1679, 1826
GlaI GCGC 2 cut(s) 22, 118
GluI GCNGC 5 cut(s) 765, 1005, 1131, 1134, 2016
HaeIII GGCC 1 cut(s) 1246
HhaI GCGC 2 cut(s) 23, 119
Hin6I GCGC 2 cut(s) 21, 117
HinP1I GCGC 2 cut(s) 21, 117
HincII GTYRAC 3 cut(s) 1111, 1308, 1696
HindII GTYRAC 3 cut(s) 1111, 1308, 1696
HindIII AAGCTT 2 cut(s) 577, 2027
HpaI GTTAAC 2 cut(s) 1308, 1696
HphI GGTGA 4 cut(s) 142, 174, 626, 1745
Hpy166II GTNNAC 5 cut(s) 684, 1111, 1156, 1308, 1696
Hpy8I GTNNAC 5 cut(s) 684, 1111, 1156, 1308, 1696
Hpy99I CGWCG 1 cut(s) 1550
HpyAV CCTTC 8 cut(s) 52, 145, 325, 803, 1257, 1318, 1649, 1766
HpyCH4III ACNGT 5 cut(s) 154, 928, 1546, 1797, 1999
HpyCH4IV ACGT 1 cut(s) 1704
HpyF10VI GCNNNNNNNGC 6 cut(s) 704, 773, 788, 901, 1634, 1845
HpyF3I CTNAG 7 cut(s) 186, 393, 466, 725, 846, 1116, 1709
HpySE526I ACGT 1 cut(s) 1704
HspAI GCGC 2 cut(s) 21, 117
Ksp22I TGATCA 2 cut(s) 367, 669
KspAI GTTAAC 2 cut(s) 1308, 1696
Kzo9I GATC 5 cut(s) 367, 550, 669, 1317, 1822
LguI GCTCTTC 1 cut(s) 736
LmnI GCTCC 2 cut(s) 465, 1723
Lsp1109I GCAGC 5 cut(s) 751, 1016, 1117, 1120, 2002
LweI GCATC 4 cut(s) 433, 778, 1599, 2067
MabI ACCWGGT 1 cut(s) 25
MaeI CTAG 7 cut(s) 282, 512, 803, 1137, 1403, 1679, 1826
MaeII ACGT 1 cut(s) 1704
MaeIII GTNAC 4 cut(s) 15, 79, 154, 1902
MalI GATC 5 cut(s) 369, 552, 671, 1319, 1824
MboI GATC 5 cut(s) 367, 550, 669, 1317, 1822
MhlI GDGCHC 3 cut(s) 71, 335, 1997
MluCI AATT 8 cut(s) 251, 414, 447, 743, 1103, 1273, 1448, 1744
MlyI GAGTC 4 cut(s) 640, 1106, 1371, 1881
MmeI TCCRAC 4 cut(s) 375, 989, 1022, 1393
Mph1103I ATGCAT 2 cut(s) 1383, 1531
MroXI GAANNNNTTC 1 cut(s) 199
MseI TTAA 6 cut(s) 8, 483, 1307, 1695, 1776, 2025
MslI CAYNNNNRTG 2 cut(s) 1296, 1881
MspA1I CMGCKG 1 cut(s) 1369
MspR9I CCNGG 4 cut(s) 27, 816, 1486, 1915
Mva1269I GAATGC 2 cut(s) 1391, 1531
MvaI CCWGG 4 cut(s) 27, 816, 1486, 1915
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 6 cut(s) 704, 773, 788, 901, 1634, 1845
NdeII GATC 5 cut(s) 367, 550, 669, 1317, 1822
NmuCI GTSAC 2 cut(s) 154, 1902
NsiI ATGCAT 2 cut(s) 1383, 1531
NspI RCATGY 2 cut(s) 117, 1579
PaeI GCATGC 1 cut(s) 117
PaeR7I CTCGAG 1 cut(s) 1889
PagI TCATGA 2 cut(s) 627, 1320
PciI ACATGT 1 cut(s) 1575
PciSI GCTCTTC 1 cut(s) 736
PctI GAATGC 2 cut(s) 1391, 1531
PdmI GAANNNNTTC 1 cut(s) 199
PfeI GAWTC 7 cut(s) 74, 470, 545, 1080, 1179, 1399, 1750
PflFI GACNNNGTC 1 cut(s) 1544
PflMI CCANNNNNTGG 1 cut(s) 950
PkrI GCNGC 5 cut(s) 766, 1006, 1132, 1135, 2017
PleI GAGTC 4 cut(s) 639, 1106, 1370, 1881
PpsI GAGTC 4 cut(s) 639, 1106, 1370, 1881
PpuMI RGGWCCY 2 cut(s) 168, 1013
PscI ACATGT 1 cut(s) 1575
Psp124BI GAGCTC 2 cut(s) 71, 335
Psp1406I AACGTT 1 cut(s) 1704
Psp5II RGGWCCY 2 cut(s) 168, 1013
Psp6I CCWGG 4 cut(s) 25, 814, 1484, 1913
PspGI CCWGG 4 cut(s) 25, 814, 1484, 1913
PspPI GGNCC 5 cut(s) 168, 971, 1013, 1477, 1917
PspPPI RGGWCCY 2 cut(s) 168, 1013
PspXI VCTCGAGB 1 cut(s) 1889
PstNI CAGNNNCTG 1 cut(s) 1845
PsyI GACNNNGTC 1 cut(s) 1544
RsaI GTAC 4 cut(s) 199, 683, 1121, 1934
RsaNI GTAC 4 cut(s) 198, 682, 1120, 1933
RseI CAYNNNNRTG 2 cut(s) 1296, 1881
SacI GAGCTC 2 cut(s) 71, 335
SapI GCTCTTC 1 cut(s) 736
SaqAI TTAA 6 cut(s) 8, 483, 1307, 1695, 1776, 2025
SatI GCNGC 5 cut(s) 765, 1005, 1131, 1134, 2016
Sau3AI GATC 5 cut(s) 367, 550, 669, 1317, 1822
Sau96I GGNCC 5 cut(s) 168, 971, 1013, 1477, 1917
ScaI AGTACT 2 cut(s) 199, 1121
SchI GAGTC 4 cut(s) 640, 1106, 1371, 1881
ScrFI CCNGG 4 cut(s) 27, 816, 1486, 1915
SduI GDGCHC 3 cut(s) 71, 335, 1997
SexAI ACCWGGT 1 cut(s) 25
SfaNI GCATC 4 cut(s) 433, 778, 1599, 2067
SfcI CTRYAG 1 cut(s) 708
Sfr274I CTCGAG 1 cut(s) 1889
SinI GGWCC 5 cut(s) 168, 971, 1013, 1477, 1917
SlaI CTCGAG 1 cut(s) 1889
SmiMI CAYNNNNRTG 2 cut(s) 1296, 1881
SmlI CTYRAG 3 cut(s) 1331, 1436, 1889
SmoI CTYRAG 3 cut(s) 1331, 1436, 1889
SphI GCATGC 1 cut(s) 117
Sse9I AATT 8 cut(s) 251, 414, 447, 743, 1103, 1273, 1448, 1744
SsiI CCGC 4 cut(s) 506, 1369, 1570, 1786
SspMI CTAG 7 cut(s) 282, 512, 803, 1137, 1403, 1679, 1826
SstI GAGCTC 2 cut(s) 71, 335
StyD4I CCNGG 4 cut(s) 25, 814, 1484, 1913
TaaI ACNGT 5 cut(s) 154, 928, 1546, 1797, 1999
TaiI ACGT 1 cut(s) 1707
TaqI TCGA 5 cut(s) 881, 1076, 1170, 1870, 1890
TasI AATT 8 cut(s) 251, 414, 447, 743, 1103, 1273, 1448, 1744
TatI WGTACW 2 cut(s) 197, 1119
TfiI GAWTC 7 cut(s) 74, 470, 545, 1080, 1179, 1399, 1750
Tru1I TTAA 6 cut(s) 8, 483, 1307, 1695, 1776, 2025
Tru9I TTAA 6 cut(s) 8, 483, 1307, 1695, 1776, 2025
TscAI CASTG 4 cut(s) 480, 841, 1911, 2002
TseFI GTSAC 2 cut(s) 154, 1902
TseI GCWGC 5 cut(s) 764, 1004, 1130, 1133, 2015
Tsp45I GTSAC 2 cut(s) 154, 1902
TspDTI ATGAA 6 cut(s) 1337, 1398, 1461, 1631, 1676, 1797
TspGWI ACGGA 4 cut(s) 41, 67, 1276, 1646
TspRI CASTG 4 cut(s) 480, 841, 1911, 2002
Tth111I GACNNNGTC 1 cut(s) 1544
Van91I CCANNNNNTGG 1 cut(s) 950
VpaK11BI GGWCC 5 cut(s) 168, 971, 1013, 1477, 1917
XapI RAATTY 3 cut(s) 1103, 1448, 1744
XbaI TCTAGA 3 cut(s) 511, 802, 1678
XceI RCATGY 2 cut(s) 117, 1579
XhoI CTCGAG 1 cut(s) 1889
XmnI GAANNNNTTC 1 cut(s) 199
XspI CTAG 7 cut(s) 282, 512, 803, 1137, 1403, 1679, 1826
ZrmI AGTACT 2 cut(s) 199, 1121
Zsp2I ATGCAT 2 cut(s) 1383, 1531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.