Rh2BG391000

C-5 cytosine-specific DNA methylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000338
Physical Location & Seq
Forward (+)
55486920 .. 55492405
5486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG391000.1

Sequence Viewer

Length: 1116 bp
ATGAAATATCCAGGAGAAGAAAATACAGATGCAATTTTGGAAATGCTCCTCACATATTCGGCTCTAGAAAGTTCAACTCATGAACAGAAACAAATGGATTCTGATCACTTCTCAGATTATGTGGAAAGCTTTCTAGATGGTTTTTCAGATATGGATAGCTGTGATGATGAGGAAATCATAAGTCATACATCTGAGGAGGAAAAACTAATGTCCTTGATCACAATGGGGTACACAGAAGAGGAGGCTTCCATGGCAGTAGAAAGATGTGGTCATAACTCCTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCAATTGGCAAAGGAAGAAGATGCCCTTCTTCCTCTTGAAGAGAAGCCCAGACTAAAACACGTGCACACTGATCCTAAGAAGAAGAGGAAAATTATTGTATCTGATATATCGAAAAGGAAAAGGCAGATGGGGCACATAGACACAGATACTGAGACACTTTGCCTCCCAAATCCAATGATTGGGTTTGGGATTCCAACTGGAGCATGGAAATCTAACAGAATTCTTCCAAAGGCAGCCATAGGGCCTCCATATTTTTATTATGAGAATGTCGCCCTTACTCCTAAAGGGGTTTGGGACACTATATCCAAATTCCTATATGATGTTCCCCCTGAGTTTGTTGATTCAAAACATTTCTGTTCTGCTGCTAGAAAAAGGGGTTACGTCCATAATCTTCCTACTGAGAACAGATTTCCTCTTGTTCCGCTCCCCCCACAAACCATACATGAGGCATTTCCCTTGACAAAAAAATGGTGGCCTTCTTGGGACATAAGAACAAAGCTAAACTGTATAGTAACTTGCTATTCTAGTGCAAAGTTATCAGAGAGGATAATGAAGCTTCTCGAGGGCTGTGATGGCATTCCACCAGAACGAGTCCAGCGGGCTGTTATACATGAATGCCGTAAGTGGAATCTATTGTGGGTTGGAAGAAATAGGGTTGCCCCACTTGAGCCTGATGAAGTGGAAATGCTCATGGGGTTTCCAAAGGATCACACAAGGGGAATGAGCCGCACTGATAGATACAAATCACTTGGCAATGCATTTCAGGTACTGAGTTCTGTCTACTTTAGAGTTCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

371

Amino Acids

42.5

Weight (kDa)

5.79

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 494
AccBSI CCGCTC 1 cut(s) 739
AccI GTMKAC 1 cut(s) 1095
AciI CCGC 3 cut(s) 737, 913, 1042
AclWI GGATC 2 cut(s) 380, 1029
AcsI RAATTY 2 cut(s) 534, 623
AcvI CACGTG 1 cut(s) 376
AfaI GTAC 2 cut(s) 230, 1083
AfiI CCNNNNNNNGG 1 cut(s) 494
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 3 cut(s) 75, 353, 660
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 4 cut(s) 129, 159, 814, 871
AluI AGCT 4 cut(s) 129, 159, 814, 871
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 461
Alw44I GTGCAC 1 cut(s) 377
AlwI GGATC 2 cut(s) 380, 1029
AlwNI CAGNNNCTG 2 cut(s) 464, 1084
Ama87I CYCGRG 1 cut(s) 875
AoxI GGCC 2 cut(s) 557, 788
ApaLI GTGCAC 1 cut(s) 377
ApeKI GCWGC 3 cut(s) 311, 548, 677
ApoI RAATTY 2 cut(s) 534, 623
Asp700I GAANNNNTTC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 557
AvaI CYCGRG 1 cut(s) 875
BaeGI GKGCMC 2 cut(s) 381, 450
BbrPI CACGTG 1 cut(s) 376
Bbv12I GWGCWC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 298, 560, 664
BccI CCATC 3 cut(s) 131, 436, 881
BceAI ACGGC 1 cut(s) 918
BciT130I CCWGG 1 cut(s) 12
BclI TGATCA 2 cut(s) 103, 216
BcoDI GTCTC 1 cut(s) 461
BfaI CTAG 4 cut(s) 65, 134, 681, 840
BisI GCNGC 4 cut(s) 312, 549, 678, 1042
BlsI GCNGC 4 cut(s) 313, 550, 679, 1043
Bme1390I CCNGG 1 cut(s) 12
BmeT110I CYCGRG 1 cut(s) 875
BmgT120I GGNCC 1 cut(s) 557
BmrFI CCNGG 1 cut(s) 12
BmsI GCATC 2 cut(s) 19, 325
BpmI CTGGAG 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 1001
BsaAI YACGTR 1 cut(s) 376
BsaBI GATNNNNATC 2 cut(s) 102, 1057
BsaJI CCNNGG 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 1 cut(s) 494
Bse1I ACTGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 1075
Bse8I GATNNNNATC 2 cut(s) 102, 1057
BseBI CCWGG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 249
BseJI GATNNNNATC 2 cut(s) 102, 1057
BseLI CCNNNNNNNGG 1 cut(s) 494
BseMI GCAATG 1 cut(s) 1075
BseMII CTCAG 6 cut(s) 126, 183, 456, 636, 705, 1076
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 4 cut(s) 38, 209, 254, 268
BseSI GKGCMC 2 cut(s) 381, 450
BseXI GCAGC 3 cut(s) 298, 560, 664
BshFI GGCC 2 cut(s) 559, 790
BsiHKAI GWGCWC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 875
BslFI GGGAC 2 cut(s) 623, 812
BslI CCNNNNNNNGG 1 cut(s) 494
BsmAI GTCTC 1 cut(s) 461
BsmFI GGGAC 2 cut(s) 623, 812
BsmI GAATGC 2 cut(s) 891, 935
BsnI GGCC 2 cut(s) 559, 790
BsoBI CYCGRG 1 cut(s) 875
Bsp1286I GDGCHC 2 cut(s) 381, 450
Bsp143I GATC 4 cut(s) 103, 216, 385, 1021
Bsp19I CCATGG 1 cut(s) 249
BspACI CCGC 3 cut(s) 737, 913, 1042
BspANI GGCC 2 cut(s) 559, 790
BspCNI CTCAG 6 cut(s) 125, 184, 457, 637, 706, 1077
BspHI TCATGA 1 cut(s) 79
BspPI GGATC 2 cut(s) 380, 1029
BsrBI CCGCTC 1 cut(s) 739
BsrDI GCAATG 1 cut(s) 1075
BsrI ACTGG 1 cut(s) 517
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 4 cut(s) 103, 216, 385, 1021
BssT1I CCWWGG 1 cut(s) 249
Bst2UI CCWGG 1 cut(s) 12
Bst4CI ACNGT 1 cut(s) 821
Bst6I CTCTTC 3 cut(s) 231, 348, 392
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 915
BstDEI CTNAG 7 cut(s) 112, 192, 390, 465, 645, 714, 1085
BstDSI CCRYGG 1 cut(s) 249
BstKTI GATC 4 cut(s) 106, 219, 388, 1024
BstMAI GTCTC 1 cut(s) 461
BstMBI GATC 4 cut(s) 103, 216, 385, 1021
BstMWI GCNNNNNNNGC 4 cut(s) 251, 320, 445, 888
BstNI CCWGG 1 cut(s) 12
BstSCI CCNGG 1 cut(s) 10
BstSLI GKGCMC 2 cut(s) 381, 450
BstV1I GCAGC 3 cut(s) 298, 560, 664
BsuRI GGCC 2 cut(s) 559, 790
BtgI CCRYGG 1 cut(s) 249
BtsIMutI CAGTG 2 cut(s) 381, 1044
Cac8I GCNNGC 1 cut(s) 915
CaiI CAGNNNCTG 2 cut(s) 464, 1084
CciI TCATGA 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 557
Csp6I GTAC 2 cut(s) 229, 1082
CviAII CATG 6 cut(s) 80, 250, 519, 758, 926, 1006
CviQI GTAC 2 cut(s) 229, 1082
DdeI CTNAG 7 cut(s) 112, 192, 390, 465, 645, 714, 1085
DpnI GATC 4 cut(s) 105, 218, 387, 1023
DpnII GATC 4 cut(s) 103, 216, 385, 1021
Eam1104I CTCTTC 3 cut(s) 231, 348, 392
EarI CTCTTC 3 cut(s) 231, 348, 392
Eco130I CCWWGG 1 cut(s) 249
Eco72I CACGTG 1 cut(s) 376
Eco88I CYCGRG 1 cut(s) 875
EcoO109I RGGNCCY 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 534
EcoRII CCWGG 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 249
EcoT22I ATGCAT 1 cut(s) 1075
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 6 cut(s) 83, 253, 522, 761, 929, 1009
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FaqI GGGAC 2 cut(s) 623, 812
FatI CATG 6 cut(s) 79, 249, 518, 757, 925, 1005
FauI CCCGC 1 cut(s) 906
FbaI TGATCA 2 cut(s) 103, 216
FblI GTMKAC 1 cut(s) 1095
Fnu4HI GCNGC 4 cut(s) 312, 549, 678, 1042
Fsp4HI GCNGC 4 cut(s) 312, 549, 678, 1042
FspBI CTAG 4 cut(s) 65, 134, 681, 840
GluI GCNGC 4 cut(s) 312, 549, 678, 1042
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 2 cut(s) 559, 790
Hin1II CATG 6 cut(s) 83, 253, 522, 761, 929, 1009
HindIII AAGCTT 2 cut(s) 127, 869
HinfI GANTC 5 cut(s) 98, 505, 656, 906, 943
Hpy166II GTNNAC 3 cut(s) 231, 379, 1096
Hpy188I TCNGA 6 cut(s) 103, 115, 148, 193, 418, 856
Hpy188III TCNNGA 6 cut(s) 65, 80, 134, 287, 350, 875
Hpy8I GTNNAC 3 cut(s) 231, 379, 1096
HpyAV CCTTC 2 cut(s) 350, 801
HpyCH4III ACNGT 1 cut(s) 821
HpyCH4IV ACGT 2 cut(s) 375, 696
HpyCH4V TGCA 4 cut(s) 32, 379, 845, 1073
HpyF10VI GCNNNNNNNGC 4 cut(s) 251, 320, 445, 888
HpyF3I CTNAG 7 cut(s) 112, 192, 390, 465, 645, 714, 1085
HpySE526I ACGT 2 cut(s) 375, 696
Hsp92II CATG 6 cut(s) 83, 253, 522, 761, 929, 1009
Ksp22I TGATCA 2 cut(s) 103, 216
Kzo9I GATC 4 cut(s) 103, 216, 385, 1021
LmnI GCTCC 3 cut(s) 51, 515, 744
LpnPI CCDG 9 cut(s) 24, 272, 376, 498, 657, 912, 923, 999, 1064
Lsp1109I GCAGC 3 cut(s) 298, 560, 664
LweI GCATC 2 cut(s) 19, 325
MaeI CTAG 4 cut(s) 65, 134, 681, 840
MaeII ACGT 2 cut(s) 375, 696
MaeIII GTNAC 2 cut(s) 692, 826
MalI GATC 4 cut(s) 105, 218, 387, 1023
MbiI CCGCTC 1 cut(s) 739
MboI GATC 4 cut(s) 103, 216, 385, 1021
MfeI CAATTG 1 cut(s) 317
MhlI GDGCHC 2 cut(s) 381, 450
MluCI AATT 6 cut(s) 33, 290, 317, 405, 534, 623
MlyI GAGTC 1 cut(s) 915
MmeI TCCRAC 2 cut(s) 533, 937
Mph1103I ATGCAT 1 cut(s) 1075
MroXI GAANNNNTTC 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 913
MspR9I CCNGG 1 cut(s) 12
MunI CAATTG 1 cut(s) 317
Mva1269I GAATGC 2 cut(s) 891, 935
MvaI CCWGG 1 cut(s) 12
MwoI GCNNNNNNNGC 4 cut(s) 251, 320, 445, 888
NcoI CCATGG 1 cut(s) 249
NdeII GATC 4 cut(s) 103, 216, 385, 1021
NlaIII CATG 6 cut(s) 83, 253, 522, 761, 929, 1009
NsiI ATGCAT 1 cut(s) 1075
PaeR7I CTCGAG 1 cut(s) 875
PagI TCATGA 1 cut(s) 79
PctI GAATGC 2 cut(s) 891, 935
PdmI GAANNNNTTC 1 cut(s) 129
PfeI GAWTC 4 cut(s) 98, 505, 656, 943
PflMI CCANNNNNTGG 1 cut(s) 494
PfoI TCCNGGA 1 cut(s) 10
PkrI GCNGC 4 cut(s) 313, 550, 679, 1043
PleI GAGTC 1 cut(s) 914
PmaCI CACGTG 1 cut(s) 376
PmlI CACGTG 1 cut(s) 376
PpsI GAGTC 1 cut(s) 914
Ppu21I YACGTR 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 10
PspCI CACGTG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 10
PspPI GGNCC 1 cut(s) 557
PstNI CAGNNNCTG 2 cut(s) 464, 1084
RsaI GTAC 2 cut(s) 230, 1083
RsaNI GTAC 2 cut(s) 229, 1082
SatI GCNGC 4 cut(s) 312, 549, 678, 1042
Sau3AI GATC 4 cut(s) 103, 216, 385, 1021
Sau96I GGNCC 1 cut(s) 557
SchI GAGTC 1 cut(s) 915
ScrFI CCNGG 1 cut(s) 12
SduI GDGCHC 2 cut(s) 381, 450
SetI ASST 7 cut(s) 131, 161, 378, 699, 816, 873, 1083
SfaNI GCATC 2 cut(s) 19, 325
Sfr274I CTCGAG 1 cut(s) 875
SlaI CTCGAG 1 cut(s) 875
SmlI CTYRAG 2 cut(s) 875, 980
SmoI CTYRAG 2 cut(s) 875, 980
Sse9I AATT 6 cut(s) 33, 290, 317, 405, 534, 623
SsiI CCGC 3 cut(s) 737, 913, 1042
SspMI CTAG 4 cut(s) 65, 134, 681, 840
StyD4I CCNGG 1 cut(s) 10
StyI CCWWGG 1 cut(s) 249
TaaI ACNGT 1 cut(s) 821
TaiI ACGT 2 cut(s) 378, 699
TaqI TCGA 2 cut(s) 425, 876
TasI AATT 6 cut(s) 33, 290, 317, 405, 534, 623
TauI GCSGC 1 cut(s) 1044
TfiI GAWTC 4 cut(s) 98, 505, 656, 943
TscAI CASTG 2 cut(s) 388, 1051
TseI GCWGC 3 cut(s) 311, 548, 677
TspDTI ATGAA 5 cut(s) 17, 96, 881, 942, 1005
TspRI CASTG 2 cut(s) 388, 1051
Van91I CCANNNNNTGG 1 cut(s) 494
VneI GTGCAC 1 cut(s) 377
XapI RAATTY 2 cut(s) 534, 623
XbaI TCTAGA 2 cut(s) 64, 133
XcmI CCANNNNNNNNNTGG 1 cut(s) 516
XhoI CTCGAG 1 cut(s) 875
XmiI GTMKAC 1 cut(s) 1095
XmnI GAANNNNTTC 1 cut(s) 129
XspI CTAG 4 cut(s) 65, 134, 681, 840
Zsp2I ATGCAT 1 cut(s) 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.