Rh2CG370600

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000338
Physical Location & Seq
Forward (+)
50291694 .. 50297305
5612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG370600.1

Sequence Viewer

Length: 1707 bp
ATGTGTTTTTACATTGCTAGGTTGAAATTTGGAGAAGTACAAGCGAAGCTTAGAGCTTACAGAGAAATGGATGGAAGTACTTCTGGTGTAGAAGACAATGATATTGATTGGAACACAGAAGATGAGCTTGAATTGGACAACTTCACTTTTTCTCCTTGTTCTAGTTTGCTACTTACTAGTGGCGAAGCTAATGCCGTCTCTGGGGAGTACCTTCTGTATTTTAGGTCTATTCGTCGTACTTGCCATGTTCTAGAAAGTTCTTCCCAAGAACTGCAACAAACCGATTCAGATCACTTTTCCTCTGATTATGATGGAAGCTTTTTGGATGACTTTTCAGATTTGGAAAGCTCTGGTGATGAGGAAATCATGAGTCCTACATCCGAGGAGGAAAAGCTACTCATGTCCTTGATCAAAATGGGGTACACAGAAGAGGAGGCTTCCATAGCTATAGAAAGATGTGGTCTTAGCTCTTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCATTTGGCAAAGGCAGAAGATGCCCTTCTTCCTCTAGAAGAGAAGCCTGGACTAAAACATTTGCACACTGATTATCCTAAGAAGAAAAGGAAACTTTTGGTTGATGAAGTGTCGAAAAGGAAAAGGCAAAATAGGCTCGTAGGCGAGGATAATGAGACAGTTCGTCTCCCAAATCCAATGATTGGGTTTGGTATTCCAACTGGACCATGCCAATCTTACAGAAATCTTCCAAAGGCAGCCATAGGACCTCCCTATTTTTATTATGAGAATGTAGCCCTCACTCCTAAAGGGGTTTGGGACACTATATCGAGATTCCTATATGATGTTCCACCTGAATTTGTTGACTCTAAGTACTTCTGTGCTGCTGCTAGAAAAAGAGGTTATGTTCACAATCTTCCAATCGAGAATAGAATCCCTCTCGTTCCACTTCCCCCACAAACCATAGATGAGGCATTGCCCATGTCAAGAAAGTGGTGGCCTTCTTGGGACACACGGACAAAGCTAAATTGCTTACAAACTTGCCATTCAAGTGCAAAGCTAACAGAGAGGATCATGAAGGCTCTTGAGGGCTATGATGACATTCCACCAGAGAGAGTCCAGCGGGGTGTAATGCATGAATGCCGTAAGTGGAATCTAGTGTGGGTTGGAAAAAATAGGGTCGCCCCACTTGAGCCTGATGAATTTGAAATGCTTTTGGGGTTTCCAAAGGACCACACCAGGGGAATAAGCAGGACTGATAGATATAAATCACTCGGGAATGCATTTCAGATTGACACCGTCGCATACCACTTCTCTGTTTTGCGAGACATGTTTCCCAATGGCATCAATGTCCTCTCCCTTTTCTCTGGGATAGGTGGGGCAGAAATAGCCCTTCATCGGCTTGGTATCCGTATGAAGAATGTTGTGTCTCTAGAGATTTCAGAAGTTAACAGAAACGTTCTGAGGTGTTGGTGGGAGCAAACAAACCAGAGGGGGAATTTGATTCACCTTCCTGATGTGCAAGAGCTTAATGCTGACCGGTTGGAACAGTTTATGAGTTCATTTGGTGGCTTTGATCTAGTAGTTGGTGGCAGTCCCTGCAACAATCTTACAGGTAGCAATCGATATCATAGAGATGGACTTGAGGGTAAAGAATCTTCCCTATTCTATCATTTCTTTCGTATACTGGACTTGGTTAAGTCTATTATGGCAGGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

568

Amino Acids

64.61

Weight (kDa)

5.41

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 445 - 559 3e-08 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 689
AccI GTMKAC 1 cut(s) 1669
AciI CCGC 1 cut(s) 1108
AclI AACGTT 1 cut(s) 1443
AclWI GGATC 1 cut(s) 1064
AcsI RAATTY 4 cut(s) 26, 842, 1187, 1483
AfaI GTAC 6 cut(s) 39, 79, 209, 238, 422, 860
AfiI CCNNNNNNNGG 4 cut(s) 201, 689, 1225, 1383
AflIII ACRYGT 1 cut(s) 1314
AgeI ACCGGT 1 cut(s) 1524
AgsI TTSAA 4 cut(s) 25, 131, 1035, 1193
AhdI GACNNNNNGTC 1 cut(s) 669
AhlI ACTAGT 1 cut(s) 176
AjnI CCWGG 2 cut(s) 553, 1223
Alw26I GTCTC 5 cut(s) 202, 656, 677, 1305, 1419
AlwI GGATC 1 cut(s) 1064
AlwNI CAGNNNCTG 1 cut(s) 1584
Ama87I CYCGRG 1 cut(s) 1259
AoxI GGCC 1 cut(s) 983
ApeKI GCWGC 4 cut(s) 503, 743, 869, 872
ApoI RAATTY 4 cut(s) 26, 842, 1187, 1483
AsiGI ACCGGT 1 cut(s) 1524
Asp700I GAANNNNTTC 1 cut(s) 79
AspS9I GGNCC 3 cut(s) 710, 752, 1216
AsuHPI GGTGA 2 cut(s) 365, 1484
AvaI CYCGRG 1 cut(s) 1259
AvaII GGWCC 3 cut(s) 710, 752, 1216
BarI GAAGNNNNNNTAC 2 cut(s) 1627, 1659
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 4 cut(s) 490, 755, 856, 859
BccI CCATC 3 cut(s) 65, 305, 1616
BceAI ACGGC 2 cut(s) 179, 1113
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 2 cut(s) 555, 1225
BciVI GTATCC 1 cut(s) 1403
BclI TGATCA 1 cut(s) 408
BcoDI GTCTC 5 cut(s) 202, 656, 677, 1305, 1419
BcuI ACTAGT 1 cut(s) 176
BfaI CTAG 9 cut(s) 18, 162, 177, 251, 542, 876, 1142, 1418, 1565
BfmI CTRYAG 1 cut(s) 447
BfuI GTATCC 1 cut(s) 1403
BisI GCNGC 4 cut(s) 504, 744, 870, 873
BlsI GCNGC 4 cut(s) 505, 745, 871, 874
BmcAI AGTACT 2 cut(s) 79, 860
Bme1390I CCNGG 2 cut(s) 555, 1225
Bme18I GGWCC 3 cut(s) 710, 752, 1216
BmeRI GACNNNNNGTC 1 cut(s) 669
BmeT110I CYCGRG 1 cut(s) 1259
BmgT120I GGNCC 3 cut(s) 710, 752, 1216
BmrFI CCNGG 2 cut(s) 555, 1225
BmsI GCATC 2 cut(s) 517, 1338
BpiI GAAGAC 1 cut(s) 99
BpuEI CTTGAG 3 cut(s) 1091, 1196, 1649
Bsa29I ATCGAT 1 cut(s) 1609
BsaBI GATNNNNATC 2 cut(s) 288, 1252
BsaJI CCNNGG 2 cut(s) 381, 1224
BsaWI WCCGGW 1 cut(s) 1524
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 4 cut(s) 201, 689, 1225, 1383
Bse118I RCCGGY 1 cut(s) 1524
Bse1I ACTGG 2 cut(s) 712, 1677
Bse3DI GCAATG 2 cut(s) 12, 959
Bse8I GATNNNNATC 2 cut(s) 288, 1252
BseBI CCWGG 2 cut(s) 555, 1225
BseCI ATCGAT 1 cut(s) 1609
BseDI CCNNGG 2 cut(s) 381, 1224
BseGI GGATG 3 cut(s) 76, 331, 377
BseJI GATNNNNATC 2 cut(s) 288, 1252
BseLI CCNNNNNNNGG 4 cut(s) 201, 689, 1225, 1383
BseMI GCAATG 2 cut(s) 12, 959
BseMII CTCAG 1 cut(s) 1439
BseNI ACTGG 2 cut(s) 712, 1677
BseRI GAGGAG 2 cut(s) 398, 446
BseXI GCAGC 4 cut(s) 490, 755, 856, 859
BshFI GGCC 1 cut(s) 985
BshTI ACCGGT 1 cut(s) 1524
BshVI ATCGAT 1 cut(s) 1609
BsiHKCI CYCGRG 1 cut(s) 1259
BsiSI CCGG 1 cut(s) 1525
BslFI GGGAC 3 cut(s) 818, 1007, 1566
BslI CCNNNNNNNGG 4 cut(s) 201, 689, 1225, 1383
BsmAI GTCTC 5 cut(s) 202, 656, 677, 1305, 1419
BsmBI CGTCTC 2 cut(s) 202, 677
BsmFI GGGAC 3 cut(s) 818, 1007, 1566
BsmI GAATGC 2 cut(s) 1130, 1270
BsnI GGCC 1 cut(s) 985
BsoBI CYCGRG 1 cut(s) 1259
Bsp143I GATC 4 cut(s) 289, 408, 1056, 1561
BspACI CCGC 1 cut(s) 1108
BspANI GGCC 1 cut(s) 985
BspCNI CTCAG 1 cut(s) 1440
BspDI ATCGAT 1 cut(s) 1609
BspHI TCATGA 2 cut(s) 366, 1059
BspPI GGATC 1 cut(s) 1064
BspQI GCTCTTC 1 cut(s) 475
BsrDI GCAATG 2 cut(s) 12, 959
BsrFI RCCGGY 1 cut(s) 1524
BsrI ACTGG 2 cut(s) 712, 1677
BssAI RCCGGY 1 cut(s) 1524
BssECI CCNNGG 2 cut(s) 381, 1224
BssMI GATC 4 cut(s) 289, 408, 1056, 1561
BssNAI GTATAC 1 cut(s) 1670
Bst1107I GTATAC 1 cut(s) 1670
Bst2UI CCWGG 2 cut(s) 555, 1225
Bst4CI ACNGT 3 cut(s) 667, 1285, 1536
Bst6I CTCTTC 3 cut(s) 423, 475, 540
BstAPI GCANNNNNTGC 2 cut(s) 527, 1584
BstDEI CTNAG 5 cut(s) 50, 464, 585, 855, 1448
BstF5I GGATG 3 cut(s) 76, 331, 377
BstKTI GATC 4 cut(s) 292, 411, 1059, 1564
BstMAI GTCTC 5 cut(s) 202, 656, 677, 1305, 1419
BstMBI GATC 4 cut(s) 289, 408, 1056, 1561
BstMWI GCNNNNNNNGC 6 cut(s) 443, 512, 527, 640, 1373, 1584
BstNI CCWGG 2 cut(s) 555, 1225
BstNSI RCATGY 1 cut(s) 1318
BstSCI CCNGG 2 cut(s) 553, 1223
BstSFI CTRYAG 1 cut(s) 447
BstV1I GCAGC 4 cut(s) 490, 755, 856, 859
BstV2I GAAGAC 1 cut(s) 99
BstZ17I GTATAC 1 cut(s) 1670
Bsu15I ATCGAT 1 cut(s) 1609
BsuI GTATCC 1 cut(s) 1403
BsuRI GGCC 1 cut(s) 985
BsuTUI ATCGAT 1 cut(s) 1609
BtsCI GGATG 3 cut(s) 76, 331, 377
BtsIMutI CAGTG 1 cut(s) 573
CaiI CAGNNNCTG 1 cut(s) 1584
CciI TCATGA 2 cut(s) 366, 1059
Cfr10I RCCGGY 1 cut(s) 1524
Cfr13I GGNCC 3 cut(s) 710, 752, 1216
ClaI ATCGAT 1 cut(s) 1609
Csp6I GTAC 6 cut(s) 38, 78, 208, 237, 421, 859
CspAI ACCGGT 1 cut(s) 1524
CviAII CATG 8 cut(s) 245, 367, 400, 714, 967, 1060, 1121, 1315
CviQI GTAC 6 cut(s) 38, 78, 208, 237, 421, 859
DdeI CTNAG 5 cut(s) 50, 464, 585, 855, 1448
DpnI GATC 4 cut(s) 291, 410, 1058, 1563
DpnII GATC 4 cut(s) 289, 408, 1056, 1561
DriI GACNNNNNGTC 1 cut(s) 669
Eam1104I CTCTTC 3 cut(s) 423, 475, 540
Eam1105I GACNNNNNGTC 1 cut(s) 669
EarI CTCTTC 3 cut(s) 423, 475, 540
Eco32I GATATC 1 cut(s) 1613
Eco47I GGWCC 3 cut(s) 710, 752, 1216
Eco88I CYCGRG 1 cut(s) 1259
EcoO109I RGGNCCY 1 cut(s) 752
EcoRII CCWGG 2 cut(s) 553, 1223
EcoRV GATATC 1 cut(s) 1613
EcoT22I ATGCAT 2 cut(s) 1122, 1270
Esp3I CGTCTC 2 cut(s) 202, 677
FaeI CATG 8 cut(s) 248, 370, 403, 717, 970, 1063, 1124, 1318
FalI AAGNNNNNCTT 6 cut(s) 33, 65, 111, 143, 516, 548
FaqI GGGAC 3 cut(s) 818, 1007, 1566
FatI CATG 8 cut(s) 244, 366, 399, 713, 966, 1059, 1120, 1314
FauI CCCGC 1 cut(s) 1101
FbaI TGATCA 1 cut(s) 408
FblI GTMKAC 1 cut(s) 1669
Fnu4HI GCNGC 4 cut(s) 504, 744, 870, 873
FokI GGATG 3 cut(s) 83, 338, 364
Fsp4HI GCNGC 4 cut(s) 504, 744, 870, 873
FspBI CTAG 9 cut(s) 18, 162, 177, 251, 542, 876, 1142, 1418, 1565
GluI GCNGC 4 cut(s) 504, 744, 870, 873
HaeIII GGCC 1 cut(s) 985
HapII CCGG 1 cut(s) 1525
Hin1II CATG 8 cut(s) 248, 370, 403, 717, 970, 1063, 1124, 1318
HincII GTYRAC 2 cut(s) 850, 1435
HindII GTYRAC 2 cut(s) 850, 1435
HindIII AAGCTT 2 cut(s) 47, 316
HinfI GANTC 9 cut(s) 284, 370, 819, 851, 918, 1101, 1138, 1489, 1640
HpaI GTTAAC 1 cut(s) 1435
HpaII CCGG 1 cut(s) 1525
HphI GGTGA 2 cut(s) 365, 1484
Hpy166II GTNNAC 5 cut(s) 423, 850, 895, 1435, 1670
Hpy188I TCNGA 7 cut(s) 289, 304, 337, 382, 1275, 1429, 1449
Hpy8I GTNNAC 5 cut(s) 423, 850, 895, 1435, 1670
Hpy99I CGWCG 2 cut(s) 237, 1289
HpyAV CCTTC 6 cut(s) 221, 542, 996, 1057, 1388, 1505
HpyCH4III ACNGT 3 cut(s) 667, 1285, 1536
HpyCH4IV ACGT 1 cut(s) 1443
HpyCH4V TGCA 7 cut(s) 274, 571, 1040, 1120, 1268, 1507, 1587
HpyF10VI GCNNNNNNNGC 6 cut(s) 443, 512, 527, 640, 1373, 1584
HpyF3I CTNAG 5 cut(s) 50, 464, 585, 855, 1448
HpySE526I ACGT 1 cut(s) 1443
Hsp92II CATG 8 cut(s) 248, 370, 403, 717, 970, 1063, 1124, 1318
Ksp22I TGATCA 1 cut(s) 408
KspAI GTTAAC 1 cut(s) 1435
Kzo9I GATC 4 cut(s) 289, 408, 1056, 1561
LguI GCTCTTC 1 cut(s) 475
LmnI GCTCC 1 cut(s) 1462
Lsp1109I GCAGC 4 cut(s) 490, 755, 856, 859
LweI GCATC 2 cut(s) 517, 1338
MaeI CTAG 9 cut(s) 18, 162, 177, 251, 542, 876, 1142, 1418, 1565
MaeII ACGT 1 cut(s) 1443
MalI GATC 4 cut(s) 291, 410, 1058, 1563
MboI GATC 4 cut(s) 289, 408, 1056, 1561
MluCI AATT 7 cut(s) 26, 131, 482, 842, 1012, 1187, 1483
MlyI GAGTC 3 cut(s) 379, 845, 1110
MmeI TCCRAC 3 cut(s) 728, 1132, 1509
Mph1103I ATGCAT 2 cut(s) 1122, 1270
MroXI GAANNNNTTC 1 cut(s) 79
MseI TTAA 3 cut(s) 1434, 1515, 1683
MslI CAYNNNNRTG 2 cut(s) 1035, 1620
MspA1I CMGCKG 1 cut(s) 1108
MspI CCGG 1 cut(s) 1525
MspR9I CCNGG 2 cut(s) 555, 1225
Mva1269I GAATGC 2 cut(s) 1130, 1270
MvaI CCWGG 2 cut(s) 555, 1225
MwoI GCNNNNNNNGC 6 cut(s) 443, 512, 527, 640, 1373, 1584
NdeII GATC 4 cut(s) 289, 408, 1056, 1561
NlaIII CATG 8 cut(s) 248, 370, 403, 717, 970, 1063, 1124, 1318
NsiI ATGCAT 2 cut(s) 1122, 1270
NspI RCATGY 1 cut(s) 1318
PagI TCATGA 2 cut(s) 366, 1059
PciI ACATGT 1 cut(s) 1314
PciSI GCTCTTC 1 cut(s) 475
PctI GAATGC 2 cut(s) 1130, 1270
PdmI GAANNNNTTC 1 cut(s) 79
PfeI GAWTC 6 cut(s) 284, 819, 918, 1138, 1489, 1640
PflFI GACNNNGTC 1 cut(s) 1283
PflMI CCANNNNNTGG 1 cut(s) 689
PinAI ACCGGT 1 cut(s) 1524
PkrI GCNGC 4 cut(s) 505, 745, 871, 874
PleI GAGTC 3 cut(s) 378, 845, 1109
PpsI GAGTC 3 cut(s) 378, 845, 1109
PpuMI RGGWCCY 1 cut(s) 752
PscI ACATGT 1 cut(s) 1314
Psp1406I AACGTT 1 cut(s) 1443
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 2 cut(s) 553, 1223
PspGI CCWGG 2 cut(s) 553, 1223
PspPI GGNCC 3 cut(s) 710, 752, 1216
PspPPI RGGWCCY 1 cut(s) 752
PstNI CAGNNNCTG 1 cut(s) 1584
PsyI GACNNNGTC 1 cut(s) 1283
RsaI GTAC 6 cut(s) 39, 79, 209, 238, 422, 860
RsaNI GTAC 6 cut(s) 38, 78, 208, 237, 421, 859
RseI CAYNNNNRTG 2 cut(s) 1035, 1620
SapI GCTCTTC 1 cut(s) 475
SaqAI TTAA 3 cut(s) 1434, 1515, 1683
SatI GCNGC 4 cut(s) 504, 744, 870, 873
Sau3AI GATC 4 cut(s) 289, 408, 1056, 1561
Sau96I GGNCC 3 cut(s) 710, 752, 1216
ScaI AGTACT 2 cut(s) 79, 860
SchI GAGTC 3 cut(s) 379, 845, 1110
ScrFI CCNGG 2 cut(s) 555, 1225
SfaNI GCATC 2 cut(s) 517, 1338
SfcI CTRYAG 1 cut(s) 447
SinI GGWCC 3 cut(s) 710, 752, 1216
SmiMI CAYNNNNRTG 2 cut(s) 1035, 1620
SmlI CTYRAG 3 cut(s) 1070, 1175, 1628
SmoI CTYRAG 3 cut(s) 1070, 1175, 1628
SpeI ACTAGT 1 cut(s) 176
Sse9I AATT 7 cut(s) 26, 131, 482, 842, 1012, 1187, 1483
SsiI CCGC 1 cut(s) 1108
SspMI CTAG 9 cut(s) 18, 162, 177, 251, 542, 876, 1142, 1418, 1565
StyD4I CCNGG 2 cut(s) 553, 1223
TaaI ACNGT 3 cut(s) 667, 1285, 1536
TaiI ACGT 1 cut(s) 1446
TaqI TCGA 4 cut(s) 620, 815, 909, 1609
TasI AATT 7 cut(s) 26, 131, 482, 842, 1012, 1187, 1483
TatI WGTACW 3 cut(s) 37, 77, 858
TfiI GAWTC 6 cut(s) 284, 819, 918, 1138, 1489, 1640
Tru1I TTAA 3 cut(s) 1434, 1515, 1683
Tru9I TTAA 3 cut(s) 1434, 1515, 1683
TscAI CASTG 1 cut(s) 580
TseI GCWGC 4 cut(s) 503, 743, 869, 872
TspDTI ATGAA 7 cut(s) 627, 1076, 1137, 1200, 1370, 1415, 1536
TspGWI ACGGA 2 cut(s) 1015, 1385
TspRI CASTG 1 cut(s) 580
Tth111I GACNNNGTC 1 cut(s) 1283
Van91I CCANNNNNTGG 1 cut(s) 689
VpaK11BI GGWCC 3 cut(s) 710, 752, 1216
XapI RAATTY 4 cut(s) 26, 842, 1187, 1483
XbaI TCTAGA 3 cut(s) 250, 541, 1417
XceI RCATGY 1 cut(s) 1318
XmiI GTMKAC 1 cut(s) 1669
XmnI GAANNNNTTC 1 cut(s) 79
XspI CTAG 9 cut(s) 18, 162, 177, 251, 542, 876, 1142, 1418, 1565
ZrmI AGTACT 2 cut(s) 79, 860
Zsp2I ATGCAT 2 cut(s) 1122, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.