RchiOBHm_Chr2g0136791

C-5 cytosine-specific DNA methylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000338
Physical Location & Seq
Forward (+)
54086363 .. 54090268
3906 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50757

Sequence Viewer

Length: 1026 bp
ATGGATGGAAGTAATTCTAGTGTAGATGACAATGATGTTGACTGGAACACAGATGATGAAATCGACAACTTCACTCTCTCGCCTTCTTCGAGTGTGATACATCCTTGTGGAGAAGCTAATGTGCTCTCTTGGGAGGTGAGCTCAGCTCCAACTTCTTCCAATACCAAAGTTCTTGATCAGTTTATAGCGATGGGATTTTCTAAGAAGATGGTTGCCAATGCAATTCAGAAACATGGAGAAGAAAATGCAGATGCAATTTTGGAAATGCTCCTCACAAATTCGGCTCTAGAAAGTTCAACTCATGAACAGAAACAAATGGATTCTGATCACTTCTCAGATTATGTGGAAAGCTTTCTAGATGGTTTTTCAGATATGGATAGCTGTGATGATGAGGAAATCATAAGTCATACATTTGAGGAGGAAAAACTAATGTCCTTGATCACAATGGGGTACACAGAAGAGGAGGCTTCCGTGGCAGTAGAAAGATGTGGTCATAACTCCTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCAATTGGCAAAGGAAGAAGATGCCCTTCTTCCTCTTGAAGAGAAGCCCAGACTAAAACATGTGCACACTGATCCTAAGAAGAAGAGGAAAATTATTGTATCTGATATATCGAAAAGGAAAAGGCAGATGGGGCACATAGACACAGATACTGAGACACTTTGCCTCCCAAATCCAATGATTGGGTTTGGGATTCCAACTGGAGCATGGAAATCTAACAGAATTCTTCCAAAGGCAGCCATAGGGCCTCCATATTTTTATTATGAGAATGTCGCCCTTACTCCTAAAGGGGTTTGGGACACTATATCCAAATTCCTATATGATGTTCCCCCTGAGTTTGTTGATTCAAAACATTTCTGTTCTGCTGCTAGAAAAAGGGGTTACGTCCATAATCTTCCTACTGAGAACAGATTTCCTCTTGTTCCGCTCCCCCCACAAACCATACATGAGGCATTTCCCTTGACAAAAAATGGTGGCCTTCTTGGGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

341

Amino Acids

37.97

Weight (kDa)

4.8

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 716
AccBSI CCGCTC 1 cut(s) 961
AciI CCGC 1 cut(s) 959
AclWI GGATC 1 cut(s) 602
AcsI RAATTY 3 cut(s) 277, 756, 845
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 716
AflIII ACRYGT 1 cut(s) 595
AgsI TTSAA 3 cut(s) 297, 575, 882
AluBI AGCT 5 cut(s) 116, 141, 146, 351, 381
AluI AGCT 5 cut(s) 116, 141, 146, 351, 381
Alw21I GWGCWC 3 cut(s) 126, 143, 603
Alw26I GTCTC 1 cut(s) 683
Alw44I GTGCAC 1 cut(s) 599
AlwI GGATC 1 cut(s) 602
AlwNI CAGNNNCTG 1 cut(s) 686
AoxI GGCC 2 cut(s) 779, 1009
ApaLI GTGCAC 1 cut(s) 599
ApeKI GCWGC 3 cut(s) 533, 770, 899
ApoI RAATTY 3 cut(s) 277, 756, 845
Asp700I GAANNNNTTC 2 cut(s) 13, 351
AspS9I GGNCC 1 cut(s) 779
AsuHPI GGTGA 1 cut(s) 148
BaeGI GKGCMC 2 cut(s) 603, 672
BanII GRGCYC 1 cut(s) 143
Bbv12I GWGCWC 3 cut(s) 126, 143, 603
BbvI GCAGC 3 cut(s) 520, 782, 886
BccI CCATC 4 cut(s) 184, 202, 353, 658
BclI TGATCA 3 cut(s) 175, 325, 438
BcoDI GTCTC 1 cut(s) 683
BfaI CTAG 4 cut(s) 18, 287, 356, 903
BisI GCNGC 3 cut(s) 534, 771, 900
BlpI GCTNAGC 1 cut(s) 142
BlsI GCNGC 3 cut(s) 535, 772, 901
BmgT120I GGNCC 1 cut(s) 779
BmsI GCATC 2 cut(s) 241, 547
BplI GAGNNNNNCTC 4 cut(s) 125, 157, 130, 162
BpmI CTGGAG 1 cut(s) 756
Bpu1102I GCTNAGC 1 cut(s) 142
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 1 cut(s) 471
BsaXI ACNNNNNCTCC 2 cut(s) 684, 714
Bsc4I CCNNNNNNNGG 1 cut(s) 716
Bse1I ACTGG 2 cut(s) 47, 739
Bse8I GATNNNNATC 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 2 cut(s) 10, 100
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMII CTCAG 5 cut(s) 156, 348, 678, 858, 927
BseNI ACTGG 2 cut(s) 47, 739
BseRI GAGGAG 4 cut(s) 260, 431, 476, 490
BseSI GKGCMC 2 cut(s) 603, 672
BseXI GCAGC 3 cut(s) 520, 782, 886
BshFI GGCC 2 cut(s) 781, 1011
BsiHKAI GWGCWC 3 cut(s) 126, 143, 603
BslFI GGGAC 1 cut(s) 845
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 1 cut(s) 683
BsmFI GGGAC 1 cut(s) 845
BsnI GGCC 2 cut(s) 781, 1011
Bsp1286I GDGCHC 4 cut(s) 126, 143, 603, 672
Bsp143I GATC 4 cut(s) 175, 325, 438, 607
Bsp1720I GCTNAGC 1 cut(s) 142
BspACI CCGC 1 cut(s) 959
BspANI GGCC 2 cut(s) 781, 1011
BspCNI CTCAG 5 cut(s) 155, 347, 679, 859, 928
BspHI TCATGA 1 cut(s) 301
BspPI GGATC 1 cut(s) 602
BsrBI CCGCTC 1 cut(s) 961
BsrI ACTGG 2 cut(s) 47, 739
BssECI CCNNGG 1 cut(s) 471
BssMI GATC 4 cut(s) 175, 325, 438, 607
Bst6I CTCTTC 3 cut(s) 453, 570, 614
BstDEI CTNAG 7 cut(s) 142, 201, 334, 612, 687, 867, 936
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 2 cut(s) 10, 100
BstKTI GATC 4 cut(s) 178, 328, 441, 610
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 4 cut(s) 175, 325, 438, 607
BstMWI GCNNNNNNNGC 3 cut(s) 473, 542, 667
BstNSI RCATGY 1 cut(s) 599
BstSLI GKGCMC 2 cut(s) 603, 672
BstV1I GCAGC 3 cut(s) 520, 782, 886
BsuRI GGCC 2 cut(s) 781, 1011
BtgI CCRYGG 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 203
BtsCI GGATG 2 cut(s) 10, 100
BtsIMutI CAGTG 1 cut(s) 603
CaiI CAGNNNCTG 1 cut(s) 686
CciI TCATGA 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 779
Csp6I GTAC 1 cut(s) 451
CviAII CATG 5 cut(s) 233, 302, 596, 741, 980
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 7 cut(s) 142, 201, 334, 612, 687, 867, 936
DpnI GATC 4 cut(s) 177, 327, 440, 609
DpnII GATC 4 cut(s) 175, 325, 438, 607
Eam1104I CTCTTC 3 cut(s) 453, 570, 614
EarI CTCTTC 3 cut(s) 453, 570, 614
Ecl136II GAGCTC 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 143
Eco53kI GAGCTC 1 cut(s) 141
EcoICRI GAGCTC 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 779
EcoRI GAATTC 1 cut(s) 756
EcoT38I GRGCYC 1 cut(s) 143
FaeI CATG 5 cut(s) 236, 305, 599, 744, 983
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 1 cut(s) 845
FatI CATG 5 cut(s) 232, 301, 595, 740, 979
FbaI TGATCA 3 cut(s) 175, 325, 438
Fnu4HI GCNGC 3 cut(s) 534, 771, 900
FokI GGATG 2 cut(s) 17, 87
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 3 cut(s) 534, 771, 900
FspBI CTAG 4 cut(s) 18, 287, 356, 903
GluI GCNGC 3 cut(s) 534, 771, 900
GsuI CTGGAG 1 cut(s) 756
HaeIII GGCC 2 cut(s) 781, 1011
Hin1II CATG 5 cut(s) 236, 305, 599, 744, 983
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 349
HinfI GANTC 3 cut(s) 320, 727, 878
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 3 cut(s) 40, 453, 601
Hpy188I TCNGA 5 cut(s) 228, 325, 337, 370, 640
Hpy188III TCNNGA 6 cut(s) 173, 287, 302, 356, 509, 572
Hpy8I GTNNAC 3 cut(s) 40, 453, 601
HpyAV CCTTC 3 cut(s) 93, 572, 1022
HpyCH4IV ACGT 1 cut(s) 918
HpyCH4V TGCA 4 cut(s) 221, 248, 254, 601
HpyF10VI GCNNNNNNNGC 3 cut(s) 473, 542, 667
HpyF3I CTNAG 7 cut(s) 142, 201, 334, 612, 687, 867, 936
HpySE526I ACGT 1 cut(s) 918
Hsp92II CATG 5 cut(s) 236, 305, 599, 744, 983
Ksp22I TGATCA 3 cut(s) 175, 325, 438
Kzo9I GATC 4 cut(s) 175, 325, 438, 607
LmnI GCTCC 4 cut(s) 151, 273, 737, 966
LpnPI CCDG 5 cut(s) 28, 494, 598, 720, 879
Lsp1109I GCAGC 3 cut(s) 520, 782, 886
LweI GCATC 2 cut(s) 241, 547
MaeI CTAG 4 cut(s) 18, 287, 356, 903
MaeII ACGT 1 cut(s) 918
MaeIII GTNAC 1 cut(s) 914
MalI GATC 4 cut(s) 177, 327, 440, 609
MbiI CCGCTC 1 cut(s) 961
MboI GATC 4 cut(s) 175, 325, 438, 607
MfeI CAATTG 1 cut(s) 539
MhlI GDGCHC 4 cut(s) 126, 143, 603, 672
MluCI AATT 9 cut(s) 13, 222, 255, 277, 512, 539, 627, 756, 845
MmeI TCCRAC 2 cut(s) 173, 755
MroXI GAANNNNTTC 2 cut(s) 13, 351
MslI CAYNNNNRTG 1 cut(s) 105
MunI CAATTG 1 cut(s) 539
MwoI GCNNNNNNNGC 3 cut(s) 473, 542, 667
NdeII GATC 4 cut(s) 175, 325, 438, 607
NlaIII CATG 5 cut(s) 236, 305, 599, 744, 983
NspI RCATGY 1 cut(s) 599
PagI TCATGA 1 cut(s) 301
PciI ACATGT 1 cut(s) 595
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PdmI GAANNNNTTC 2 cut(s) 13, 351
PfeI GAWTC 3 cut(s) 320, 727, 878
PflMI CCANNNNNTGG 1 cut(s) 716
PkrI GCNGC 3 cut(s) 535, 772, 901
PscI ACATGT 1 cut(s) 595
Psp124BI GAGCTC 1 cut(s) 143
PspPI GGNCC 1 cut(s) 779
PstNI CAGNNNCTG 1 cut(s) 686
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
RseI CAYNNNNRTG 1 cut(s) 105
SacI GAGCTC 1 cut(s) 143
SatI GCNGC 3 cut(s) 534, 771, 900
Sau3AI GATC 4 cut(s) 175, 325, 438, 607
Sau96I GGNCC 1 cut(s) 779
SduI GDGCHC 4 cut(s) 126, 143, 603, 672
SetI ASST 7 cut(s) 118, 138, 143, 148, 353, 383, 921
SfaNI GCATC 2 cut(s) 241, 547
SmiMI CAYNNNNRTG 1 cut(s) 105
Sse9I AATT 9 cut(s) 13, 222, 255, 277, 512, 539, 627, 756, 845
SsiI CCGC 1 cut(s) 959
SspMI CTAG 4 cut(s) 18, 287, 356, 903
SstI GAGCTC 1 cut(s) 143
TaiI ACGT 1 cut(s) 921
TaqI TCGA 3 cut(s) 63, 89, 647
TasI AATT 9 cut(s) 13, 222, 255, 277, 512, 539, 627, 756, 845
TfiI GAWTC 3 cut(s) 320, 727, 878
TscAI CASTG 1 cut(s) 610
TseI GCWGC 3 cut(s) 533, 770, 899
TspDTI ATGAA 2 cut(s) 72, 318
TspGWI ACGGA 1 cut(s) 460
TspRI CASTG 1 cut(s) 610
Van91I CCANNNNNTGG 1 cut(s) 716
VneI GTGCAC 1 cut(s) 599
XapI RAATTY 3 cut(s) 277, 756, 845
XbaI TCTAGA 2 cut(s) 286, 355
XceI RCATGY 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 738
XmnI GAANNNNTTC 2 cut(s) 13, 351
XspI CTAG 4 cut(s) 18, 287, 356, 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.