RLG00000030366

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000338
Physical Location & Seq
Forward (+)
60144116 .. 60145279
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030366

Sequence Viewer

Length: 513 bp
ATGAAGGCTCTTATAGACTATGACAAAGATGAAATCCCACCTGAAAGTGTCCGTCGCTATGTTCTTTATGAATGCCGCAAGTGGAATCTTGTTTGGGTAGGAAAAAAAAAAAAGGTGGCCTCACTTGAGCCTGATGAAGTAGAAATGCTTTTGAGTTTCTCCAGAGACCACACAAGGGGAATAAGCAGGACAGATAGATACAAGTCCCTGGGAAATTCGTTCCAGACAGTTCCATTTGCTTTGGTTTTTTGTTCTGTTCACAAGCTTGATCTAAATAGCTCTTTATCGGCTTGGTATCCCAATGAAGAATGTTGTGTCAGTGGCGATTTCAGAGAGCATTATATCCGTTCCTTTGGGGGGTTTGATCTGGTGATTGGTGGCAGTCCATGTAACAATCTTGTGGGTAGCAACAGACACCATCGGGATGGGCTTCAGGGTAAAGAGTCTTCTCTTTTTTATGACTATTTTCGTATATTGGATTTGGTCAAGGGTATTATGGCAAGATACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

171

Amino Acids

19.66

Weight (kDa)

8.24

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 76
AcsI RAATTY 1 cut(s) 214
AcuI CTGAAG 1 cut(s) 416
AfiI CCNNNNNNNGG 2 cut(s) 175, 357
AjnI CCWGG 1 cut(s) 207
AluBI AGCT 2 cut(s) 265, 279
AluI AGCT 2 cut(s) 265, 279
Alw26I GTCTC 1 cut(s) 159
AoxI GGCC 1 cut(s) 117
ApoI RAATTY 1 cut(s) 214
AsuHPI GGTGA 1 cut(s) 382
BarI GAAGNNNNNNTAC 2 cut(s) 430, 462
BbsI GAAGAC 1 cut(s) 438
BccI CCATC 2 cut(s) 419, 426
BciT130I CCWGG 1 cut(s) 209
BciVI GTATCC 1 cut(s) 306
BcoDI GTCTC 1 cut(s) 159
BfuI GTATCC 1 cut(s) 306
BisI GCNGC 1 cut(s) 76
BlsI GCNGC 1 cut(s) 77
Bme1390I CCNGG 1 cut(s) 209
BmrFI CCNGG 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 438
BpmI CTGGAG 1 cut(s) 145
BpuEI CTTGAG 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 159
BsaJI CCNNGG 2 cut(s) 207, 208
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 357
BseBI CCWGG 1 cut(s) 209
BseDI CCNNGG 2 cut(s) 207, 208
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 2 cut(s) 175, 357
BshFI GGCC 1 cut(s) 119
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 2 cut(s) 175, 357
BsmAI GTCTC 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 1 cut(s) 119
Bso31I GGTCTC 1 cut(s) 159
Bsp143I GATC 2 cut(s) 268, 364
BspACI CCGC 1 cut(s) 76
BspANI GGCC 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 159
BssECI CCNNGG 2 cut(s) 207, 208
BssMI GATC 2 cut(s) 268, 364
Bst2UI CCWGG 1 cut(s) 209
Bst4CI ACNGT 1 cut(s) 229
BstF5I GGATG 1 cut(s) 430
BstKTI GATC 2 cut(s) 271, 367
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 2 cut(s) 268, 364
BstNI CCWGG 1 cut(s) 209
BstSCI CCNGG 1 cut(s) 207
BstV2I GAAGAC 1 cut(s) 438
BstXI CCANNNNNNTGG 1 cut(s) 425
BsuI GTATCC 1 cut(s) 306
BsuRI GGCC 1 cut(s) 119
BtsCI GGATG 1 cut(s) 430
BtsIMutI CAGTG 1 cut(s) 325
CviAII CATG 1 cut(s) 387
CviJI RGCY 7 cut(s) 8, 119, 130, 265, 279, 290, 430
CviKI_1 RGCY 7 cut(s) 8, 119, 130, 265, 279, 290, 430
DpnI GATC 2 cut(s) 270, 366
DpnII GATC 2 cut(s) 268, 364
Eco31I GGTCTC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 207
FaeI CATG 1 cut(s) 390
FaiI YATR 9 cut(s) 14, 21, 60, 69, 342, 388, 459, 473, 497
FaqI GGGAC 1 cut(s) 190
FatI CATG 1 cut(s) 386
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 1 cut(s) 437
Fsp4HI GCNGC 1 cut(s) 76
GluI GCNGC 1 cut(s) 76
GsuI CTGGAG 1 cut(s) 145
HaeIII GGCC 1 cut(s) 119
Hin1II CATG 1 cut(s) 390
HindIII AAGCTT 1 cut(s) 263
HinfI GANTC 2 cut(s) 85, 443
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 1 cut(s) 332
Hpy188III TCNNGA 3 cut(s) 162, 223, 422
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 1 cut(s) 57
HpyCH4III ACNGT 1 cut(s) 229
Hsp92II CATG 1 cut(s) 390
Kzo9I GATC 2 cut(s) 268, 364
LpnPI CCDG 9 cut(s) 54, 144, 172, 175, 194, 221, 236, 353, 419
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 270, 366
MboI GATC 2 cut(s) 268, 364
MboII GAAGA 2 cut(s) 317, 438
MfeI CAATTG 1 cut(s) 508
MluCI AATT 2 cut(s) 214, 508
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 1 cut(s) 130
MslI CAYNNNNRTG 1 cut(s) 423
MspR9I CCNGG 1 cut(s) 209
MunI CAATTG 1 cut(s) 508
Mva1269I GAATGC 1 cut(s) 77
MvaI CCWGG 1 cut(s) 209
NdeII GATC 2 cut(s) 268, 364
NlaIII CATG 1 cut(s) 390
PasI CCCWGGG 1 cut(s) 208
PctI GAATGC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 85
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
Psp6I CCWGG 1 cut(s) 207
PspGI CCWGG 1 cut(s) 207
RseI CAYNNNNRTG 1 cut(s) 423
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 2 cut(s) 268, 364
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 1 cut(s) 209
SetI ASST 4 cut(s) 43, 117, 267, 281
SmiMI CAYNNNNRTG 1 cut(s) 423
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 2 cut(s) 214, 508
SsiI CCGC 1 cut(s) 76
StyD4I CCNGG 1 cut(s) 207
TaaI ACNGT 1 cut(s) 229
TasI AATT 2 cut(s) 214, 508
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 1 cut(s) 85
TscAI CASTG 1 cut(s) 325
TspDTI ATGAA 5 cut(s) 17, 45, 84, 150, 318
TspGWI ACGGA 2 cut(s) 41, 335
TspRI CASTG 1 cut(s) 325
XapI RAATTY 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.