pycom17g02950

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000338
Physical Location & Seq
Reverse (-)
1943970 .. 1950769
6800 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g02950.3

Sequence Viewer

Length: 2196 bp
ATGATTTTGCGTGTTATTTTCCTTCTTATTATCTTCCTTGCATGCAGATATATATTCCACAAGCGCATGCCTCCTGAAGCAGTAGATCTTGTGTCAAGATTGCTTCAGTATTCTCCGAGCCTAAGATTCACTGCTGTAAGTAGTGCCATTTTCCTTCAAAGTATTTATGTGCAAACTGCAAAGGAATGGGGATTTATTCACATCTCTCTCAGGCTTTCTCTCTCTAGATTTCTCTCCACCGAGCTCTTCTCGTTTCCCTGCGAAATTCTGCCGGCCGGGTCTTTTTTTCAGCGGCGGAGCTCCCTTCGCATGCGCCGTACAAGGCCGCAAGGGGGGGTTTTGACGGAGGCGAAGCGCATTGCCGGTGTTAACGAGGGGAGCTTCGGGATCAGTGAGGCCCCTTCTGCGGAGGATGGTGAAGACAGTAATGATTTTGACTGGAATACTGATGATGAGCTGGAAATTGAGAACTTTACGTTGTCTACTTCTTCAAGTTTGACAGTTGGTGGAGAAGCTATTGTGAGATCTGGGGAGTCGAGCTCATCGGCCGGTCCCTCCAATACCAAAGTTCTTGATCACTTTGTGGCAATGGGATTTTCTGAAAAGATGGTTGTCAAAGCAATTCAGGAACATGGAGAAGAAAATACAGATTCAATTCTGGAAACCCTCCTCACATATTCGGCTCTTGAGAGTTCTCCTGAAGAAAAGCAGGAAGTAGAAACGCTCCAGGAAGTAGAACCACAGGAAGTAGAACCGCAGGAAGCTGATTCTGATGACTTCTCTCTTGATGATTACGATGAGAGCTTTCTGGATGATTTTTCAGACTTGGATAGCTGTTCTGATACCGAGGATATCATAAATCCTACATCGGAGAAAGGGAAGATAAGGGCCTTGGTCAATATGGGGTACACAATGGAGGAGGCCTCAATAGCAATCGAAAGATGTGGTACAGACTCCACTGTTGCTGAGTTGACTGATTTTATATGTGCTGCTCAAATGGCACGGGCGGAAGATGCCCTTCTTCCGATTGAAGAGAAGCCTGGATCACATTCATACAAGAAGAGGAAACTTCTCGAGTATGAGATCCTAAAGAAGAAGAAGAGGCAGATGCGGCTTTGTGGAAACCAAGGGGATGATGATGAGACGGTTCATCTCCCAAATCCAATGGTTGGGTTTGGCACCCCAACTGATCCATATGTGCTGACACATAGGACTCTCCCAGAGGCAGCTGTAGGGCCTCCATACTTCTACTATGAGAATGTTGCACTAGCTCCTAAAGGGGTTTGGACCACCATCTCCCGCTTCCTGTATGATGTGCAGCCGGAGTTTGTGGACTCAAAGTACTTTTCTGCTGCTGCAAGGAAGAGAGGTTATGTCCACAACCTCCCCATCCAGAATAGGTTTCCTCTCATGCCACAGCCCCCAGACACCATAGCCAAGGCATTTCCCTTGACAAGGAAATGGTGGCCTTCTTGGGACAATCGGGAAAAGCTAAACTGCTTGCAAACATGTATTGCGAGTGCTAAGTTGACTGAGAGGATCAGGAAGGCTATTGAGCAATTTGATGGGGAACCACCAGAGAGAGTCCAGAAATATGTCCTTGACGAATGCCGCAAGTGGAATCTGGTGTGGGTAGGGAGAAATAAGGTCGCCCCACTTGAGCCTGATGAAGTGGAGATGCTTTTGGGGTTCCCAAAAAACCACACAAGGGGAATAAGCCGGACTGACAGGTACAAGTCACTTGGGAATTCCTTCCAGGTTGACACTGTGGCATATCATCTGTCTGTGCTCAAGGATATGTATCCCAATGGCATCAACGTCCTCTCCCTCTTTACTGGCATTGGAGGGGCAGAAGTAGCCCTTCACCGGCTTGGTGTCCCTCTGAAGAATGTTATATCTGTAGAGATCTCAGAAGTGAACAGAAACGTTGTGAGCAGTTGGTGGGAGCAAACCAACCAGAGAGGGAGGTTGCAGCACGTTGATGACGTGCAGCAACTGAATGGCGATCACCTTGAGCAGTACATGAGCGAGTTTGGCGGATTTGATCTGGTGATTGGTGGGAGCCCGTGCAACAATCTAGCAGGCAGCAATCGACATCATCGTGATGGACTGGAGGGTAAGGATTCGGCCCTTTTTTACGACTTTTTCCGAATATTGGACTTGGTGAAGAACATGATGGGAGGAAGAAACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

732

Amino Acids

82.45

Weight (kDa)

5.08

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1178
AccB7I CCANNNNNTGG 1 cut(s) 1169
AccI GTMKAC 1 cut(s) 482
AclI AACGTT 1 cut(s) 1926
AclWI GGATC 5 cut(s) 395, 1051, 1078, 1184, 1547
AcoI YGGCCR 2 cut(s) 273, 546
AcsI RAATTY 2 cut(s) 264, 1747
AcuI CTGAAG 4 cut(s) 89, 96, 720, 1904
AdeI CACNNNGTG 1 cut(s) 583
AfaI GTAC 6 cut(s) 319, 908, 949, 1343, 1733, 2021
AfiI CCNNNNNNNGG 7 cut(s) 332, 406, 1169, 1455, 1708, 1866, 2155
AflIII ACRYGT 1 cut(s) 1508
AgsI TTSAA 4 cut(s) 158, 492, 654, 1031
AjiI CACGTC 1 cut(s) 1987
AjnI CCWGG 3 cut(s) 726, 1039, 1755
Alw21I GWGCWC 4 cut(s) 246, 302, 542, 1791
Alw26I GTCTC 1 cut(s) 1136
AlwI GGATC 5 cut(s) 395, 1051, 1078, 1184, 1547
AlwNI CAGNNNCTG 1 cut(s) 1996
Ama87I CYCGRG 1 cut(s) 1073
AoxI GGCC 9 cut(s) 273, 323, 396, 546, 888, 921, 1235, 1466, 2127
ApeKI GCWGC 8 cut(s) 989, 1226, 1318, 1352, 1355, 1972, 1990, 2085
ApoI RAATTY 2 cut(s) 264, 1747
Asp700I GAANNNNTTC 1 cut(s) 1751
AspLEI GCGC 3 cut(s) 66, 315, 357
AspS9I GGNCC 6 cut(s) 397, 551, 888, 1235, 1287, 2128
AsuC2I CCSGG 1 cut(s) 277
AsuHPI GGTGA 5 cut(s) 428, 1856, 2000, 2062, 2176
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 2 cut(s) 551, 1287
BanI GGYRCC 1 cut(s) 1178
BanII GRGCYC 4 cut(s) 246, 302, 542, 2066
BbsI GAAGAC 1 cut(s) 426
Bbv12I GWGCWC 4 cut(s) 246, 302, 542, 1791
BbvI GCAGC 8 cut(s) 976, 1238, 1330, 1339, 1342, 1984, 2002, 2097
BccI CCATC 7 cut(s) 407, 601, 1301, 1397, 1559, 2099, 2170
BceAI ACGGC 1 cut(s) 300
BciT130I CCWGG 3 cut(s) 728, 1041, 1757
BciVI GTATCC 1 cut(s) 1812
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 1 cut(s) 277
BcoDI GTCTC 1 cut(s) 1136
BfaI CTAG 3 cut(s) 225, 1268, 2078
BfmI CTRYAG 2 cut(s) 1230, 1899
BfuI GTATCC 1 cut(s) 1812
BglII AGATCT 3 cut(s) 85, 524, 1905
BmcAI AGTACT 1 cut(s) 1343
Bme1390I CCNGG 4 cut(s) 277, 728, 1041, 1757
Bme18I GGWCC 2 cut(s) 551, 1287
BmeT110I CYCGRG 1 cut(s) 1073
BmgBI CACGTC 1 cut(s) 1987
BmgT120I GGNCC 6 cut(s) 397, 551, 888, 1235, 1287, 2128
BmiI GGNNCC 6 cut(s) 399, 553, 1180, 1572, 1691, 2063
BmrFI CCNGG 4 cut(s) 277, 728, 1041, 1757
BmsI GCATC 4 cut(s) 1003, 1098, 1668, 1821
BpiI GAAGAC 1 cut(s) 426
BplI GAGNNNNNCTC 6 cut(s) 233, 265, 524, 556, 908, 940
BpmI CTGGAG 2 cut(s) 710, 2132
BpuEI CTTGAG 4 cut(s) 707, 1679, 1775, 2033
BpuMI CCSGG 1 cut(s) 277
BsaBI GATNNNNATC 1 cut(s) 1800
BsaJI CCNNGG 4 cut(s) 846, 891, 1126, 1437
BsaXI ACNNNNNCTCC 2 cut(s) 1808, 1838
Bsc4I CCNNNNNNNGG 7 cut(s) 332, 406, 1169, 1455, 1708, 1866, 2155
Bse118I RCCGGY 4 cut(s) 271, 362, 548, 1866
Bse1I ACTGG 3 cut(s) 443, 1840, 2115
Bse3DI GCAATG 2 cut(s) 357, 594
Bse8I GATNNNNATC 1 cut(s) 1800
BseBI CCWGG 3 cut(s) 728, 1041, 1757
BseDI CCNNGG 4 cut(s) 846, 891, 1126, 1437
BseGI GGATG 4 cut(s) 418, 817, 1138, 1389
BseJI GATNNNNATC 1 cut(s) 1800
BseLI CCNNNNNNNGG 7 cut(s) 332, 406, 1169, 1455, 1708, 1866, 2155
BseMI GCAATG 2 cut(s) 357, 594
BseMII CTCAG 4 cut(s) 223, 957, 1524, 1923
BseNI ACTGG 3 cut(s) 443, 1840, 2115
BseRI GAGGAG 2 cut(s) 659, 932
BseX3I CGGCCG 2 cut(s) 273, 546
BseXI GCAGC 8 cut(s) 976, 1238, 1330, 1339, 1342, 1984, 2002, 2097
BsgI GTGCAG 2 cut(s) 1337, 2009
Bsh1285I CGRYCG 2 cut(s) 276, 549
BshFI GGCC 9 cut(s) 275, 325, 398, 548, 890, 923, 1237, 1468, 2129
BshNI GGYRCC 1 cut(s) 1178
BsiEI CGRYCG 2 cut(s) 276, 549
BsiHKAI GWGCWC 4 cut(s) 246, 302, 542, 1791
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 7 cut(s) 272, 276, 363, 549, 1322, 1720, 1867
BslFI GGGAC 3 cut(s) 537, 1490, 1862
BslI CCNNNNNNNGG 7 cut(s) 332, 406, 1169, 1455, 1708, 1866, 2155
BsmAI GTCTC 1 cut(s) 1136
BsmBI CGTCTC 1 cut(s) 1136
BsmFI GGGAC 3 cut(s) 537, 1490, 1862
BsmI GAATGC 1 cut(s) 1613
BsnI GGCC 9 cut(s) 275, 325, 398, 548, 890, 923, 1237, 1468, 2129
BsoBI CYCGRG 1 cut(s) 1073
Bsp1286I GDGCHC 5 cut(s) 246, 302, 542, 1791, 2066
BspANI GGCC 9 cut(s) 275, 325, 398, 548, 890, 923, 1237, 1468, 2129
BspCNI CTCAG 4 cut(s) 222, 958, 1525, 1922
BspLI GGNNCC 6 cut(s) 399, 553, 1180, 1572, 1691, 2063
BspPI GGATC 5 cut(s) 395, 1051, 1078, 1184, 1547
BspQI GCTCTTC 1 cut(s) 251
BspT107I GGYRCC 1 cut(s) 1178
BsrDI GCAATG 2 cut(s) 357, 594
BsrFI RCCGGY 4 cut(s) 271, 362, 548, 1866
BsrI ACTGG 3 cut(s) 443, 1840, 2115
BssAI RCCGGY 4 cut(s) 271, 362, 548, 1866
BssECI CCNNGG 4 cut(s) 846, 891, 1126, 1437
BssT1I CCWWGG 3 cut(s) 891, 1126, 1437
Bst2UI CCWGG 3 cut(s) 728, 1041, 1757
Bst4CI ACNGT 5 cut(s) 425, 502, 961, 1147, 1768
Bst6I CTCTTC 5 cut(s) 251, 1026, 1055, 1094, 1358
BstC8I GCNNGC 6 cut(s) 43, 68, 273, 311, 1502, 2083
BstDEI CTNAG 6 cut(s) 122, 209, 966, 1524, 1533, 1909
BstENI CCTNNNNNAGG 1 cut(s) 1453
BstF5I GGATG 4 cut(s) 418, 817, 1138, 1389
BstHHI GCGC 3 cut(s) 66, 315, 357
BstMAI GTCTC 1 cut(s) 1136
BstMCI CGRYCG 2 cut(s) 276, 549
BstMWI GCNNNNNNNGC 8 cut(s) 306, 404, 929, 998, 1013, 1111, 1856, 2034
BstNI CCWGG 3 cut(s) 728, 1041, 1757
BstNSI RCATGY 4 cut(s) 45, 70, 313, 1512
BstSCI CCNGG 4 cut(s) 275, 726, 1039, 1755
BstSFI CTRYAG 2 cut(s) 1230, 1899
BstV1I GCAGC 8 cut(s) 976, 1238, 1330, 1339, 1342, 1984, 2002, 2097
BstV2I GAAGAC 1 cut(s) 426
BstX2I RGATCY 4 cut(s) 85, 524, 1083, 1905
BstYI RGATCY 4 cut(s) 85, 524, 1083, 1905
BstZI CGGCCG 2 cut(s) 273, 546
BsuI GTATCC 1 cut(s) 1812
BsuRI GGCC 9 cut(s) 275, 325, 398, 548, 890, 923, 1237, 1468, 2129
BtrI CACGTC 1 cut(s) 1987
BtsCI GGATG 4 cut(s) 418, 817, 1138, 1389
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 4 cut(s) 129, 397, 957, 1764
Cac8I GCNNGC 6 cut(s) 43, 68, 273, 311, 1502, 2083
CaiI CAGNNNCTG 1 cut(s) 1996
CfoI GCGC 3 cut(s) 66, 315, 357
Cfr10I RCCGGY 4 cut(s) 271, 362, 548, 1866
Cfr13I GGNCC 6 cut(s) 397, 551, 888, 1235, 1287, 2128
Csp6I GTAC 6 cut(s) 318, 907, 948, 1342, 1732, 2020
CviAII CATG 8 cut(s) 42, 67, 310, 632, 1411, 1509, 2023, 2173
CviQI GTAC 6 cut(s) 318, 907, 948, 1342, 1732, 2020
DdeI CTNAG 6 cut(s) 122, 209, 966, 1524, 1533, 1909
DraIII CACNNNGTG 1 cut(s) 583
EaeI YGGCCR 2 cut(s) 273, 546
EagI CGGCCG 2 cut(s) 273, 546
Eam1104I CTCTTC 5 cut(s) 251, 1026, 1055, 1094, 1358
EarI CTCTTC 5 cut(s) 251, 1026, 1055, 1094, 1358
EciI GGCGGA 3 cut(s) 310, 1022, 2052
Ecl136II GAGCTC 3 cut(s) 244, 300, 540
EclXI CGGCCG 2 cut(s) 273, 546
Eco130I CCWWGG 3 cut(s) 891, 1126, 1437
Eco147I AGGCCT 1 cut(s) 923
Eco24I GRGCYC 4 cut(s) 246, 302, 542, 2066
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 2 cut(s) 551, 1287
Eco52I CGGCCG 2 cut(s) 273, 546
Eco53kI GAGCTC 3 cut(s) 244, 300, 540
Eco57I CTGAAG 4 cut(s) 89, 96, 720, 1904
Eco88I CYCGRG 1 cut(s) 1073
EcoICRI GAGCTC 3 cut(s) 244, 300, 540
EcoNI CCTNNNNNAGG 1 cut(s) 1453
EcoO109I RGGNCCY 3 cut(s) 397, 888, 1235
EcoRI GAATTC 1 cut(s) 1747
EcoRII CCWGG 3 cut(s) 726, 1039, 1755
EcoRV GATATC 1 cut(s) 853
EcoT14I CCWWGG 3 cut(s) 891, 1126, 1437
EcoT38I GRGCYC 4 cut(s) 246, 302, 542, 2066
ErhI CCWWGG 3 cut(s) 891, 1126, 1437
Esp3I CGTCTC 1 cut(s) 1136
FaeI CATG 8 cut(s) 45, 70, 313, 635, 1414, 1512, 2026, 2176
FalI AAGNNNNNCTT 4 cut(s) 1002, 1034, 1845, 1877
FaqI GGGAC 3 cut(s) 537, 1490, 1862
FatI CATG 8 cut(s) 41, 66, 309, 631, 1410, 1508, 2022, 2172
FauI CCCGC 1 cut(s) 1307
FauNDI CATATG 1 cut(s) 1195
FbaI TGATCA 1 cut(s) 574
FblI GTMKAC 1 cut(s) 482
FokI GGATG 4 cut(s) 425, 824, 1145, 1376
FriOI GRGCYC 4 cut(s) 246, 302, 542, 2066
FspBI CTAG 3 cut(s) 225, 1268, 2078
GlaI GCGC 3 cut(s) 65, 314, 356
GsuI CTGGAG 2 cut(s) 710, 2132
HaeIII GGCC 9 cut(s) 275, 325, 398, 548, 890, 923, 1237, 1468, 2129
HapII CCGG 7 cut(s) 272, 276, 363, 549, 1322, 1720, 1867
HhaI GCGC 3 cut(s) 66, 315, 357
Hin1II CATG 8 cut(s) 45, 70, 313, 635, 1414, 1512, 2026, 2176
Hin6I GCGC 3 cut(s) 64, 313, 355
HinP1I GCGC 3 cut(s) 64, 313, 355
HincII GTYRAC 4 cut(s) 370, 972, 1530, 1762
HindII GTYRAC 4 cut(s) 370, 972, 1530, 1762
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 7 cut(s) 272, 276, 363, 549, 1322, 1720, 1867
HphI GGTGA 5 cut(s) 428, 1856, 2000, 2062, 2176
Hpy166II GTNNAC 9 cut(s) 370, 483, 909, 972, 1333, 1378, 1530, 1762, 1918
Hpy8I GTNNAC 9 cut(s) 370, 483, 909, 972, 1333, 1378, 1530, 1762, 1918
HpyAV CCTTC 9 cut(s) 32, 164, 314, 411, 1028, 1479, 1540, 1762, 1871
HpyCH4III ACNGT 5 cut(s) 425, 502, 961, 1147, 1768
HpyCH4IV ACGT 5 cut(s) 476, 1818, 1926, 1977, 1986
HpyF10VI GCNNNNNNNGC 8 cut(s) 306, 404, 929, 998, 1013, 1111, 1856, 2034
HpyF3I CTNAG 6 cut(s) 122, 209, 966, 1524, 1533, 1909
HpySE526I ACGT 5 cut(s) 476, 1818, 1926, 1977, 1986
Hsp92II CATG 8 cut(s) 45, 70, 313, 635, 1414, 1512, 2026, 2176
HspAI GCGC 3 cut(s) 64, 313, 355
KroI GCCGGC 1 cut(s) 271
KroNI GCCGGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 574
KspAI GTTAAC 1 cut(s) 370
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 7 cut(s) 297, 305, 378, 729, 1276, 1945, 2061
Lsp1109I GCAGC 8 cut(s) 976, 1238, 1330, 1339, 1342, 1984, 2002, 2097
LweI GCATC 4 cut(s) 1003, 1098, 1668, 1821
MaeI CTAG 3 cut(s) 225, 1268, 2078
MaeII ACGT 5 cut(s) 476, 1818, 1926, 1977, 1986
MaeIII GTNAC 1 cut(s) 1737
MfeI CAATTG 1 cut(s) 2191
MflI RGATCY 4 cut(s) 85, 524, 1083, 1905
MhlI GDGCHC 5 cut(s) 246, 302, 542, 1791, 2066
MluCI AATT 7 cut(s) 264, 462, 621, 654, 1559, 1747, 2191
MlyI GAGTC 5 cut(s) 542, 947, 1207, 1328, 1593
MroNI GCCGGC 1 cut(s) 271
MroXI GAANNNNTTC 1 cut(s) 1751
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 3 cut(s) 1980, 2100, 2103
MspA1I CMGCKG 2 cut(s) 292, 1229
MspI CCGG 7 cut(s) 272, 276, 363, 549, 1322, 1720, 1867
MspR9I CCNGG 4 cut(s) 277, 728, 1041, 1757
MunI CAATTG 1 cut(s) 2191
Mva1269I GAATGC 1 cut(s) 1613
MvaI CCWGG 3 cut(s) 728, 1041, 1757
MwoI GCNNNNNNNGC 8 cut(s) 306, 404, 929, 998, 1013, 1111, 1856, 2034
NaeI GCCGGC 1 cut(s) 273
NciI CCSGG 1 cut(s) 277
NdeI CATATG 1 cut(s) 1195
NgoMIV GCCGGC 1 cut(s) 271
NlaIII CATG 8 cut(s) 45, 70, 313, 635, 1414, 1512, 2026, 2176
NlaIV GGNNCC 6 cut(s) 399, 553, 1180, 1572, 1691, 2063
NmuCI GTSAC 1 cut(s) 1737
NspI RCATGY 4 cut(s) 45, 70, 313, 1512
PaeI GCATGC 3 cut(s) 45, 70, 313
PaeR7I CTCGAG 1 cut(s) 1073
PceI AGGCCT 1 cut(s) 923
PciI ACATGT 1 cut(s) 1508
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 3 cut(s) 313, 1983, 2098
PctI GAATGC 1 cut(s) 1613
PdiI GCCGGC 1 cut(s) 273
PdmI GAANNNNTTC 1 cut(s) 1751
PfeI GAWTC 5 cut(s) 126, 650, 767, 1621, 2123
PflMI CCANNNNNTGG 1 cut(s) 1169
PfoI TCCNGGA 1 cut(s) 726
PleI GAGTC 5 cut(s) 541, 947, 1207, 1328, 1592
PpsI GAGTC 5 cut(s) 541, 947, 1207, 1328, 1592
PscI ACATGT 1 cut(s) 1508
Psp124BI GAGCTC 3 cut(s) 246, 302, 542
Psp1406I AACGTT 1 cut(s) 1926
Psp6I CCWGG 3 cut(s) 726, 1039, 1755
PspGI CCWGG 3 cut(s) 726, 1039, 1755
PspN4I GGNNCC 6 cut(s) 399, 553, 1180, 1572, 1691, 2063
PspPI GGNCC 6 cut(s) 397, 551, 888, 1235, 1287, 2128
PstNI CAGNNNCTG 1 cut(s) 1996
PsuI RGATCY 4 cut(s) 85, 524, 1083, 1905
PvuII CAGCTG 1 cut(s) 1229
RsaI GTAC 6 cut(s) 319, 908, 949, 1343, 1733, 2021
RsaNI GTAC 6 cut(s) 318, 907, 948, 1342, 1732, 2020
RseI CAYNNNNRTG 3 cut(s) 1980, 2100, 2103
SacI GAGCTC 3 cut(s) 246, 302, 542
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 369
Sau96I GGNCC 6 cut(s) 397, 551, 888, 1235, 1287, 2128
ScaI AGTACT 1 cut(s) 1343
SchI GAGTC 5 cut(s) 542, 947, 1207, 1328, 1593
ScrFI CCNGG 4 cut(s) 277, 728, 1041, 1757
SduI GDGCHC 5 cut(s) 246, 302, 542, 1791, 2066
SfaNI GCATC 4 cut(s) 1003, 1098, 1668, 1821
SfcI CTRYAG 2 cut(s) 1230, 1899
Sfr274I CTCGAG 1 cut(s) 1073
SinI GGWCC 2 cut(s) 551, 1287
SlaI CTCGAG 1 cut(s) 1073
SmiMI CAYNNNNRTG 3 cut(s) 1980, 2100, 2103
SmlI CTYRAG 5 cut(s) 686, 1073, 1658, 1790, 2012
SmoI CTYRAG 5 cut(s) 686, 1073, 1658, 1790, 2012
SphI GCATGC 3 cut(s) 45, 70, 313
Sse9I AATT 7 cut(s) 264, 462, 621, 654, 1559, 1747, 2191
SseBI AGGCCT 1 cut(s) 923
SspI AATATT 1 cut(s) 2154
SspMI CTAG 3 cut(s) 225, 1268, 2078
SstI GAGCTC 3 cut(s) 246, 302, 542
StuI AGGCCT 1 cut(s) 923
StyD4I CCNGG 4 cut(s) 275, 726, 1039, 1755
StyI CCWWGG 3 cut(s) 891, 1126, 1437
TaaI ACNGT 5 cut(s) 425, 502, 961, 1147, 1768
TaiI ACGT 5 cut(s) 479, 1821, 1929, 1980, 1989
TaqI TCGA 4 cut(s) 536, 936, 1074, 2092
TasI AATT 7 cut(s) 264, 462, 621, 654, 1559, 1747, 2191
TatI WGTACW 2 cut(s) 1341, 2019
TauI GCSGC 4 cut(s) 295, 328, 1114, 1614
TfiI GAWTC 5 cut(s) 126, 650, 767, 1621, 2123
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 4 cut(s) 136, 397, 964, 1771
TseFI GTSAC 1 cut(s) 1737
TseI GCWGC 8 cut(s) 989, 1226, 1318, 1352, 1355, 1972, 1990, 2085
Tsp45I GTSAC 1 cut(s) 1737
TspDTI ATGAA 3 cut(s) 1041, 1139, 1683
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 4 cut(s) 136, 397, 964, 1771
Van91I CCANNNNNTGG 1 cut(s) 1169
VpaK11BI GGWCC 2 cut(s) 551, 1287
XagI CCTNNNNNAGG 1 cut(s) 1453
XapI RAATTY 2 cut(s) 264, 1747
XbaI TCTAGA 1 cut(s) 224
XceI RCATGY 4 cut(s) 45, 70, 313, 1512
XhoI CTCGAG 1 cut(s) 1073
XmiI GTMKAC 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 1751
XspI CTAG 3 cut(s) 225, 1268, 2078
ZrmI AGTACT 1 cut(s) 1343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.