Rw2G031410

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000338
Physical Location & Seq
Forward (+)
51508453 .. 51513800
5348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031410.1

Sequence Viewer

Length: 1974 bp
ATGAGCGGAGTAGCTGCAAAACGTGATGAGGGAGTAGTTGAAATCTGCTGGCATGACATAATGACACGGCATGTCGTTAAGTTTGTTACGCGCACCAGGTGGCAGTCGTTCCTTCCATTTCCGTCAGCAGTATTTGAGCTCGGATTCCGTTACAGACAAGCCTGCCATCGCTTTCTCTTCTGCATGCGTTGGAATTTGGAGAAGTACAAGCGAAGCTTAGAGCTTACAGAGAAATGTACTTCTGGTGTAGAAGACAATGATATTGATTGGAACACAGAAGATGAGCTTGAATTGGACAACTTCACTTTATCTCCTTGTTCTAGTTTGCTACTTACTGGTGGCGAAGCTAATGCCTTCTCTGGGGAGGCGAGCTCATGTCCAAGTTCTTCCAACTCCAAAGTTGTTGATCAGTTTATAGGGATGGGATTTTCTAAGAAGATGGTTGAAAAAGCAATTCAGGAACATGGAGAAGAAAATACAGATGCAATTCTGGAAATGGTCCTCTCAGATTCAGTTCTAGAAAGTTCTTCCCAAGAACTGCAACAAACCGATTCAGATCACTTTTCCTCTGATTATGATGGAAGCTTTTTGGATGACTTTTCAGATTTGGAAAGCTCTGGTGATGAGGAAATCATGAGTCCTACATCCGAGGAGGAAAAGCTACTCATGTCCTTGATCAAAATGGGGTACACAGAAGAGGAGGCTTCCATAGCTATAGAAAGATGTGGTCTTAGCTCTTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCATTTGGCAAAGGCAGAAGATGCCCTTCTTCCTCTAGAACAGAAGCCTGGACTAAAACATTTGCACACTGATTATCCTAAGAAGAAAAGGAAACATTTGGTTGATCAAGTGTCGAAAAGGAAAAGGCAAAATAGGCTCATAGGCGAGGATAATGAGACAGTTCGTCTCCCAAATCCAATGATTGGGTTTGGTATTCCAACTGGACCATGCCAATCTTACAGAACTCTTCCAAAGGCAGCCATAGGACCTCCCTATTTTTATTATGAGAATGTAGCCCTCACTCCTAAAGGGGTTTGGGACACTATATCGAGATTCCTATATGATGTTCCACCTGAATTTGTTGACTCTAAGTACTTCTGTGCTGCTGCTAGAAAAAGAGGTTATGTTCACAATCTTCCAATCGAGAATAGAATCCCTCTCGTTCCACTTCCCCCACAAACCATACATGAGGCATTGCCCATGTCAAGAAAGTGGTGGCCTTCTTGGGACACACGGACAAAGCTAAATTGCTTACAAACTTGCCATTCAAGTGCAAAGTTAACAGAGAGGATCATGAAGGCTCTTGAGGGCTATGATGACATTCCACCAGAGAGAGTCCAGCGGGGTGTAATGCATGAATGCCGTAAGTGGAATCTAGTGTGGGTTGGAAAAAATAGGGTCGCCCCACTTGAGCCTGATGAATTTGAAATGCTTTTGGGGTTTCCAAAGGACCACACCAGGGGAATAAGCAGGACTGATAGATATAAATCACTCGGGAATGCATTTCAGATTGACACCGTCGCATACCACTTCTCTGTTTTGCGAGACATGTTTCCCAATGGCATCAATGTCCTCTCCCTTTTCTCTGGGATAGGTGGGGCGGAAATAGCCCTTCATCGGCTTGGTATCCGTATGAAGAATGTTGTGTCTCTAGAGATTTCAGAAGTTAACAGAAACGTTCTGAGGTGTTGGTGGGAGCAAACAAACCAGAGGGGGAATTTGATTCACCTTCCTGATGTGCGAGAGCTTAATGCTGACCGCTTGGAACAGTTTATGAGTTCATTTGGTGGCTTTGATCTAGTAGTTGGTGGCAGTCCCTGCAACAATCTTACAGGTAGCAATCGATATCATAGAGATGGACTCGAGGGTAAAGAATCTTCCCTATTCTATCATTTCTTTCGTATACTGGACTTGGTTAAGTCTATTATGGCAGGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

657

Amino Acids

74.7

Weight (kDa)

5.7

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 534 - 648 2.3e-08 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 956
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 1936
AccII CGCG 1 cut(s) 91
AciI CCGC 4 cut(s) 6, 1375, 1634, 1792
AclI AACGTT 1 cut(s) 1710
AclWI GGATC 1 cut(s) 1331
AcsI RAATTY 4 cut(s) 193, 1109, 1454, 1750
AdeI CACNNNGTG 1 cut(s) 99
AfaI GTAC 4 cut(s) 206, 238, 689, 1127
AfiI CCNNNNNNNGG 4 cut(s) 360, 956, 1492, 1650
AflIII ACRYGT 1 cut(s) 1581
AgsI TTSAA 5 cut(s) 41, 290, 446, 1302, 1460
AhdI GACNNNNNGTC 1 cut(s) 936
AjnI CCWGG 3 cut(s) 95, 820, 1490
Alw21I GWGCWC 2 cut(s) 141, 374
Alw26I GTCTC 4 cut(s) 923, 944, 1572, 1686
AlwI GGATC 1 cut(s) 1331
AlwNI CAGNNNCTG 1 cut(s) 1851
Ama87I CYCGRG 2 cut(s) 1526, 1895
AoxI GGCC 1 cut(s) 1250
ApeKI GCWGC 5 cut(s) 14, 770, 1010, 1136, 1139
ApoI RAATTY 4 cut(s) 193, 1109, 1454, 1750
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 4 cut(s) 499, 977, 1019, 1483
AsuHPI GGTGA 2 cut(s) 632, 1751
AvaI CYCGRG 2 cut(s) 1526, 1895
AvaII GGWCC 4 cut(s) 499, 977, 1019, 1483
BanII GRGCYC 2 cut(s) 141, 374
BarI GAAGNNNNNNTAC 2 cut(s) 1894, 1926
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 2 cut(s) 141, 374
BbvI GCAGC 4 cut(s) 757, 1022, 1123, 1126
BccI CCATC 5 cut(s) 174, 415, 433, 572, 1883
BceAI ACGGC 2 cut(s) 83, 1380
BcgI CGANNNNNNTGC 2 cut(s) 332, 366
BciT130I CCWGG 3 cut(s) 97, 822, 1492
BciVI GTATCC 1 cut(s) 1670
BclI TGATCA 3 cut(s) 406, 675, 877
BcoDI GTCTC 4 cut(s) 923, 944, 1572, 1686
BfaI CTAG 7 cut(s) 321, 518, 809, 1143, 1409, 1685, 1832
BfmI CTRYAG 1 cut(s) 714
BfuI GTATCC 1 cut(s) 1670
BisI GCNGC 5 cut(s) 15, 771, 1011, 1137, 1140
BlsI GCNGC 5 cut(s) 16, 772, 1012, 1138, 1141
BmcAI AGTACT 1 cut(s) 1127
Bme1390I CCNGG 3 cut(s) 97, 822, 1492
Bme18I GGWCC 4 cut(s) 499, 977, 1019, 1483
BmeRI GACNNNNNGTC 1 cut(s) 936
BmeT110I CYCGRG 2 cut(s) 1526, 1895
BmgT120I GGNCC 4 cut(s) 499, 977, 1019, 1483
BmrFI CCNGG 3 cut(s) 97, 822, 1492
BmsI GCATC 3 cut(s) 472, 784, 1605
BpiI GAAGAC 1 cut(s) 258
BplI GAGNNNNNCTC 4 cut(s) 356, 388, 1878, 1910
BpuEI CTTGAG 2 cut(s) 1358, 1463
Bsa29I ATCGAT 1 cut(s) 1876
BsaBI GATNNNNATC 2 cut(s) 555, 1519
BsaJI CCNNGG 2 cut(s) 648, 1491
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
Bsc4I CCNNNNNNNGG 4 cut(s) 360, 956, 1492, 1650
Bse1I ACTGG 3 cut(s) 340, 979, 1944
Bse3DI GCAATG 1 cut(s) 1226
Bse8I GATNNNNATC 2 cut(s) 555, 1519
BseBI CCWGG 3 cut(s) 97, 822, 1492
BseCI ATCGAT 1 cut(s) 1876
BseDI CCNNGG 2 cut(s) 648, 1491
BseGI GGATG 3 cut(s) 426, 598, 644
BseJI GATNNNNATC 2 cut(s) 555, 1519
BseLI CCNNNNNNNGG 4 cut(s) 360, 956, 1492, 1650
BseMI GCAATG 1 cut(s) 1226
BseMII CTCAG 2 cut(s) 519, 1706
BseNI ACTGG 3 cut(s) 340, 979, 1944
BseRI GAGGAG 2 cut(s) 665, 713
BseXI GCAGC 4 cut(s) 757, 1022, 1123, 1126
Bsh1236I CGCG 1 cut(s) 91
BshFI GGCC 1 cut(s) 1252
BshVI ATCGAT 1 cut(s) 1876
BsiHKAI GWGCWC 2 cut(s) 141, 374
BsiHKCI CYCGRG 2 cut(s) 1526, 1895
BslFI GGGAC 3 cut(s) 1085, 1274, 1833
BslI CCNNNNNNNGG 4 cut(s) 360, 956, 1492, 1650
BsmAI GTCTC 4 cut(s) 923, 944, 1572, 1686
BsmBI CGTCTC 1 cut(s) 944
BsmFI GGGAC 3 cut(s) 1085, 1274, 1833
BsmI GAATGC 2 cut(s) 1397, 1537
BsnI GGCC 1 cut(s) 1252
BsoBI CYCGRG 2 cut(s) 1526, 1895
Bsp1286I GDGCHC 2 cut(s) 141, 374
Bsp143I GATC 6 cut(s) 406, 556, 675, 877, 1323, 1828
BspACI CCGC 4 cut(s) 6, 1375, 1634, 1792
BspANI GGCC 1 cut(s) 1252
BspCNI CTCAG 2 cut(s) 518, 1707
BspDI ATCGAT 1 cut(s) 1876
BspFNI CGCG 1 cut(s) 91
BspHI TCATGA 2 cut(s) 633, 1326
BspPI GGATC 1 cut(s) 1331
BspQI GCTCTTC 1 cut(s) 742
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 1 cut(s) 1226
BsrI ACTGG 3 cut(s) 340, 979, 1944
BssECI CCNNGG 2 cut(s) 648, 1491
BssMI GATC 6 cut(s) 406, 556, 675, 877, 1323, 1828
BssNAI GTATAC 1 cut(s) 1937
Bst1107I GTATAC 1 cut(s) 1937
Bst2UI CCWGG 3 cut(s) 97, 822, 1492
Bst4CI ACNGT 3 cut(s) 934, 1552, 1803
Bst6I CTCTTC 4 cut(s) 182, 690, 742, 1005
BstAPI GCANNNNNTGC 2 cut(s) 794, 1851
BstC8I GCNNGC 4 cut(s) 50, 163, 185, 370
BstDEI CTNAG 7 cut(s) 217, 432, 505, 731, 852, 1122, 1715
BstF5I GGATG 3 cut(s) 426, 598, 644
BstFNI CGCG 1 cut(s) 91
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 6 cut(s) 409, 559, 678, 880, 1326, 1831
BstMAI GTCTC 4 cut(s) 923, 944, 1572, 1686
BstMBI GATC 6 cut(s) 406, 556, 675, 877, 1323, 1828
BstMWI GCNNNNNNNGC 6 cut(s) 710, 779, 794, 907, 1640, 1851
BstNI CCWGG 3 cut(s) 97, 822, 1492
BstNSI RCATGY 3 cut(s) 74, 187, 1585
BstSCI CCNGG 3 cut(s) 95, 820, 1490
BstSFI CTRYAG 1 cut(s) 714
BstUI CGCG 1 cut(s) 91
BstV1I GCAGC 4 cut(s) 757, 1022, 1123, 1126
BstV2I GAAGAC 1 cut(s) 258
BstZ17I GTATAC 1 cut(s) 1937
Bsu15I ATCGAT 1 cut(s) 1876
BsuI GTATCC 1 cut(s) 1670
BsuRI GGCC 1 cut(s) 1252
BsuTUI ATCGAT 1 cut(s) 1876
BtgZI GCGATG 1 cut(s) 152
BtsCI GGATG 3 cut(s) 426, 598, 644
BtsIMutI CAGTG 1 cut(s) 840
Cac8I GCNNGC 4 cut(s) 50, 163, 185, 370
CaiI CAGNNNCTG 1 cut(s) 1851
CciI TCATGA 2 cut(s) 633, 1326
CfoI GCGC 1 cut(s) 93
Cfr13I GGNCC 4 cut(s) 499, 977, 1019, 1483
ClaI ATCGAT 1 cut(s) 1876
CsiI ACCWGGT 1 cut(s) 95
Csp6I GTAC 4 cut(s) 205, 237, 688, 1126
CviQI GTAC 4 cut(s) 205, 237, 688, 1126
DdeI CTNAG 7 cut(s) 217, 432, 505, 731, 852, 1122, 1715
DpnI GATC 6 cut(s) 408, 558, 677, 879, 1325, 1830
DpnII GATC 6 cut(s) 406, 556, 675, 877, 1323, 1828
DraIII CACNNNGTG 1 cut(s) 99
DriI GACNNNNNGTC 1 cut(s) 936
Eam1104I CTCTTC 4 cut(s) 182, 690, 742, 1005
Eam1105I GACNNNNNGTC 1 cut(s) 936
EarI CTCTTC 4 cut(s) 182, 690, 742, 1005
EciI GGCGGA 1 cut(s) 1649
Ecl136II GAGCTC 2 cut(s) 139, 372
Eco24I GRGCYC 2 cut(s) 141, 374
Eco32I GATATC 1 cut(s) 1880
Eco47I GGWCC 4 cut(s) 499, 977, 1019, 1483
Eco53kI GAGCTC 2 cut(s) 139, 372
Eco88I CYCGRG 2 cut(s) 1526, 1895
EcoICRI GAGCTC 2 cut(s) 139, 372
EcoO109I RGGNCCY 1 cut(s) 1019
EcoRII CCWGG 3 cut(s) 95, 820, 1490
EcoRV GATATC 1 cut(s) 1880
EcoT22I ATGCAT 2 cut(s) 1389, 1537
EcoT38I GRGCYC 2 cut(s) 141, 374
Esp3I CGTCTC 1 cut(s) 944
FalI AAGNNNNNCTT 6 cut(s) 200, 232, 270, 302, 783, 815
FaqI GGGAC 3 cut(s) 1085, 1274, 1833
FauI CCCGC 1 cut(s) 1368
FbaI TGATCA 3 cut(s) 406, 675, 877
FblI GTMKAC 1 cut(s) 1936
Fnu4HI GCNGC 5 cut(s) 15, 771, 1011, 1137, 1140
FokI GGATG 3 cut(s) 433, 605, 631
FriOI GRGCYC 2 cut(s) 141, 374
Fsp4HI GCNGC 5 cut(s) 15, 771, 1011, 1137, 1140
FspBI CTAG 7 cut(s) 321, 518, 809, 1143, 1409, 1685, 1832
GlaI GCGC 1 cut(s) 92
GluI GCNGC 5 cut(s) 15, 771, 1011, 1137, 1140
HaeIII GGCC 1 cut(s) 1252
HhaI GCGC 1 cut(s) 93
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HincII GTYRAC 3 cut(s) 1117, 1314, 1702
HindII GTYRAC 3 cut(s) 1117, 1314, 1702
HindIII AAGCTT 2 cut(s) 214, 583
HpaI GTTAAC 2 cut(s) 1314, 1702
HphI GGTGA 2 cut(s) 632, 1751
Hpy166II GTNNAC 6 cut(s) 690, 1117, 1162, 1314, 1702, 1937
Hpy188I TCNGA 9 cut(s) 143, 508, 556, 571, 604, 649, 1542, 1696, 1716
Hpy8I GTNNAC 6 cut(s) 690, 1117, 1162, 1314, 1702, 1937
Hpy99I CGWCG 1 cut(s) 1556
HpyAV CCTTC 7 cut(s) 122, 364, 809, 1263, 1324, 1655, 1772
HpyCH4III ACNGT 3 cut(s) 934, 1552, 1803
HpyCH4IV ACGT 2 cut(s) 22, 1710
HpyCH4V TGCA 9 cut(s) 17, 183, 485, 541, 838, 1307, 1387, 1535, 1854
HpyF10VI GCNNNNNNNGC 6 cut(s) 710, 779, 794, 907, 1640, 1851
HpyF3I CTNAG 7 cut(s) 217, 432, 505, 731, 852, 1122, 1715
HpySE526I ACGT 2 cut(s) 22, 1710
HspAI GCGC 1 cut(s) 91
Ksp22I TGATCA 3 cut(s) 406, 675, 877
KspAI GTTAAC 2 cut(s) 1314, 1702
Kzo9I GATC 6 cut(s) 406, 556, 675, 877, 1323, 1828
LguI GCTCTTC 1 cut(s) 742
LmnI GCTCC 1 cut(s) 1729
Lsp1109I GCAGC 4 cut(s) 757, 1022, 1123, 1126
LweI GCATC 3 cut(s) 472, 784, 1605
MabI ACCWGGT 1 cut(s) 95
MaeI CTAG 7 cut(s) 321, 518, 809, 1143, 1409, 1685, 1832
MaeII ACGT 2 cut(s) 22, 1710
MaeIII GTNAC 2 cut(s) 85, 149
MalI GATC 6 cut(s) 408, 558, 677, 879, 1325, 1830
MbiI CCGCTC 1 cut(s) 6
MboI GATC 6 cut(s) 406, 556, 675, 877, 1323, 1828
MhlI GDGCHC 2 cut(s) 141, 374
MluCI AATT 9 cut(s) 193, 290, 453, 486, 749, 1109, 1279, 1454, 1750
MlyI GAGTC 4 cut(s) 646, 1112, 1377, 1887
MmeI TCCRAC 4 cut(s) 170, 414, 995, 1399
Mph1103I ATGCAT 2 cut(s) 1389, 1537
MseI TTAA 5 cut(s) 78, 1313, 1701, 1782, 1950
MslI CAYNNNNRTG 2 cut(s) 1302, 1887
MspA1I CMGCKG 1 cut(s) 1375
MspR9I CCNGG 3 cut(s) 97, 822, 1492
Mva1269I GAATGC 2 cut(s) 1397, 1537
MvaI CCWGG 3 cut(s) 97, 822, 1492
MvnI CGCG 1 cut(s) 91
MwoI GCNNNNNNNGC 6 cut(s) 710, 779, 794, 907, 1640, 1851
NdeII GATC 6 cut(s) 406, 556, 675, 877, 1323, 1828
NsiI ATGCAT 2 cut(s) 1389, 1537
NspI RCATGY 3 cut(s) 74, 187, 1585
PaeI GCATGC 1 cut(s) 187
PaeR7I CTCGAG 1 cut(s) 1895
PagI TCATGA 2 cut(s) 633, 1326
PciI ACATGT 1 cut(s) 1581
PciSI GCTCTTC 1 cut(s) 742
PctI GAATGC 2 cut(s) 1397, 1537
PfeI GAWTC 8 cut(s) 144, 509, 551, 1086, 1185, 1405, 1756, 1907
PflFI GACNNNGTC 1 cut(s) 1550
PflMI CCANNNNNTGG 1 cut(s) 956
PkrI GCNGC 5 cut(s) 16, 772, 1012, 1138, 1141
PleI GAGTC 4 cut(s) 645, 1112, 1376, 1887
PpsI GAGTC 4 cut(s) 645, 1112, 1376, 1887
PpuMI RGGWCCY 1 cut(s) 1019
PscI ACATGT 1 cut(s) 1581
Psp124BI GAGCTC 2 cut(s) 141, 374
Psp1406I AACGTT 1 cut(s) 1710
Psp5II RGGWCCY 1 cut(s) 1019
Psp6I CCWGG 3 cut(s) 95, 820, 1490
PspGI CCWGG 3 cut(s) 95, 820, 1490
PspPI GGNCC 4 cut(s) 499, 977, 1019, 1483
PspPPI RGGWCCY 1 cut(s) 1019
PspXI VCTCGAGB 1 cut(s) 1895
PstNI CAGNNNCTG 1 cut(s) 1851
PsyI GACNNNGTC 1 cut(s) 1550
RsaI GTAC 4 cut(s) 206, 238, 689, 1127
RsaNI GTAC 4 cut(s) 205, 237, 688, 1126
RseI CAYNNNNRTG 2 cut(s) 1302, 1887
SacI GAGCTC 2 cut(s) 141, 374
SapI GCTCTTC 1 cut(s) 742
SaqAI TTAA 5 cut(s) 78, 1313, 1701, 1782, 1950
SatI GCNGC 5 cut(s) 15, 771, 1011, 1137, 1140
Sau3AI GATC 6 cut(s) 406, 556, 675, 877, 1323, 1828
Sau96I GGNCC 4 cut(s) 499, 977, 1019, 1483
ScaI AGTACT 1 cut(s) 1127
SchI GAGTC 4 cut(s) 646, 1112, 1377, 1887
ScrFI CCNGG 3 cut(s) 97, 822, 1492
SduI GDGCHC 2 cut(s) 141, 374
SexAI ACCWGGT 1 cut(s) 95
SfaNI GCATC 3 cut(s) 472, 784, 1605
SfcI CTRYAG 1 cut(s) 714
Sfr274I CTCGAG 1 cut(s) 1895
SinI GGWCC 4 cut(s) 499, 977, 1019, 1483
SlaI CTCGAG 1 cut(s) 1895
SmiMI CAYNNNNRTG 2 cut(s) 1302, 1887
SmlI CTYRAG 3 cut(s) 1337, 1442, 1895
SmoI CTYRAG 3 cut(s) 1337, 1442, 1895
SphI GCATGC 1 cut(s) 187
Sse9I AATT 9 cut(s) 193, 290, 453, 486, 749, 1109, 1279, 1454, 1750
SsiI CCGC 4 cut(s) 6, 1375, 1634, 1792
SspMI CTAG 7 cut(s) 321, 518, 809, 1143, 1409, 1685, 1832
SstI GAGCTC 2 cut(s) 141, 374
StyD4I CCNGG 3 cut(s) 95, 820, 1490
TaaI ACNGT 3 cut(s) 934, 1552, 1803
TaiI ACGT 2 cut(s) 25, 1713
TaqI TCGA 5 cut(s) 887, 1082, 1176, 1876, 1896
TasI AATT 9 cut(s) 193, 290, 453, 486, 749, 1109, 1279, 1454, 1750
TatI WGTACW 3 cut(s) 204, 236, 1125
TfiI GAWTC 8 cut(s) 144, 509, 551, 1086, 1185, 1405, 1756, 1907
Tru1I TTAA 5 cut(s) 78, 1313, 1701, 1782, 1950
Tru9I TTAA 5 cut(s) 78, 1313, 1701, 1782, 1950
TscAI CASTG 1 cut(s) 847
TseI GCWGC 5 cut(s) 14, 770, 1010, 1136, 1139
TspDTI ATGAA 6 cut(s) 1343, 1404, 1467, 1637, 1682, 1803
TspGWI ACGGA 4 cut(s) 111, 137, 1282, 1652
TspRI CASTG 1 cut(s) 847
Tth111I GACNNNGTC 1 cut(s) 1550
Van91I CCANNNNNTGG 1 cut(s) 956
VpaK11BI GGWCC 4 cut(s) 499, 977, 1019, 1483
XapI RAATTY 4 cut(s) 193, 1109, 1454, 1750
XbaI TCTAGA 3 cut(s) 517, 808, 1684
XceI RCATGY 3 cut(s) 74, 187, 1585
XhoI CTCGAG 1 cut(s) 1895
XmiI GTMKAC 1 cut(s) 1936
XspI CTAG 7 cut(s) 321, 518, 809, 1143, 1409, 1685, 1832
ZrmI AGTACT 1 cut(s) 1127
Zsp2I ATGCAT 2 cut(s) 1389, 1537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.